LOCUS       NM_014500               2694 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens HIV-1 Tat specific factor 1 (HTATSF1), transcript
            variant 2, mRNA.
ACCESSION   NM_014500
VERSION     NM_014500.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Yang,F., Bian,T., Zhan,X., Chen,Z., Xing,Z., Larsen,N.A., Zhang,X.
            and Shi,Y.
  TITLE     Mechanisms of the RNA helicases DDX42 and DDX46 in human U2 snRNP
            assembly
  JOURNAL   Nat Commun 14 (1), 897 (2023)
   PUBMED   36797247
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Zhao,J., Tian,S., Guo,Q., Bao,K., Yu,G., Wang,X., Shen,X.,
            Zhang,J., Chen,J., Yang,Y., Liu,L., Li,X., Hao,J., Yang,N., Liu,Z.,
            Ai,D., Yang,J., Zhu,Y., Yao,Z., Ma,S., Zhang,K. and Shi,L.
  TITLE     A PARylation-phosphorylation cascade promotes TOPBP1 loading and
            RPA-RAD51 exchange in homologous recombination
  JOURNAL   Mol Cell 82 (14), 2571-2587 (2022)
   PUBMED   35597237
REFERENCE   3  (bases 1 to 2694)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   4  (bases 1 to 2694)
  AUTHORS   Tholen,J., Razew,M., Weis,F. and Galej,W.P.
  TITLE     Structural basis of branch site recognition by the human
            spliceosome
  JOURNAL   Science 375 (6576), 50-57 (2022)
   PUBMED   34822310
REFERENCE   5  (bases 1 to 2694)
  AUTHORS   Zhang,Z., Will,C.L., Bertram,K., Dybkov,O., Hartmuth,K.,
            Agafonov,D.E., Hofele,R., Urlaub,H., Kastner,B., Luhrmann,R. and
            Stark,H.
  TITLE     Molecular architecture of the human 17S U2 snRNP
  JOURNAL   Nature 583 (7815), 310-313 (2020)
   PUBMED   32494006
REFERENCE   6  (bases 1 to 2694)
  AUTHORS   Parada,C.A. and Roeder,R.G.
  TITLE     A novel RNA polymerase II-containing complex potentiates
            Tat-enhanced HIV-1 transcription
  JOURNAL   EMBO J 18 (13), 3688-3701 (1999)
   PUBMED   10393184
REFERENCE   7  (bases 1 to 2694)
  AUTHORS   Li,X.Y. and Green,M.R.
  TITLE     The HIV-1 Tat cellular coactivator Tat-SF1 is a general
            transcription elongation factor
  JOURNAL   Genes Dev 12 (19), 2992-2996 (1998)
   PUBMED   9765201
REFERENCE   8  (bases 1 to 2694)
  AUTHORS   Yan,D., Perriman,R., Igel,H., Howe,K.J., Neville,M. and Ares,M. Jr.
  TITLE     CUS2, a yeast homolog of human Tat-SF1, rescues function of
            misfolded U2 through an unusual RNA recognition motif
  JOURNAL   Mol Cell Biol 18 (9), 5000-5009 (1998)
   PUBMED   9710584
REFERENCE   9  (bases 1 to 2694)
  AUTHORS   Zhou,Q., Chen,D., Pierstorff,E. and Luo,K.
  TITLE     Transcription elongation factor P-TEFb mediates Tat activation of
            HIV-1 transcription at multiple stages
  JOURNAL   EMBO J 17 (13), 3681-3691 (1998)
   PUBMED   9649438
REFERENCE   10 (bases 1 to 2694)
  AUTHORS   Zhou,Q. and Sharp,P.A.
  TITLE     Tat-SF1: cofactor for stimulation of transcriptional elongation by
            HIV-1 Tat
  JOURNAL   Science 274 (5287), 605-610 (1996)
   PUBMED   8849451
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC009896.2 and BU633070.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_014500.4.
            
            Summary: The protein encoded by this gene functions as a cofactor
            for the stimulation of transcriptional elongation by HIV-1 Tat,
            which binds to the HIV-1 promoter through Tat-TAR interaction. This
            protein may also serve as a dual-function factor to couple
            transcription and splicing and to facilitate their reciprocal
            activation. Alternatively spliced transcript variants have been
            found for this gene.[provided by RefSeq, Sep 2009].
            
            Transcript Variant: This variant (2) is missing a 5' non-coding
            exon, and therefore, has a different 5' UTR compared to variant 1.
            Variants 1 and 2 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.836266.1,
                                           SRR1660807.5254.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000218364.5/ ENSP00000218364.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2679              BC009896.2         81-2759
            2680-2694           BU633070.1         15-29               c
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq26.3"
     gene            1..2694
                     /gene="HTATSF1"
                     /gene_synonym="dJ196E23.2; TAT-SF1; TATSF1"
                     /note="HIV-1 Tat specific factor 1"
                     /db_xref="GeneID:27336"
                     /db_xref="HGNC:HGNC:5276"
                     /db_xref="MIM:300346"
     exon            1..279
                     /gene="HTATSF1"
                     /gene_synonym="dJ196E23.2; TAT-SF1; TATSF1"
                     /inference="alignment:Splign:2.1.0"
     CDS             94..2361
                     /gene="HTATSF1"
                     /gene_synonym="dJ196E23.2; TAT-SF1; TATSF1"
                     /note="HIV TAT specific factor 1; cofactor required for
                     Tat activation of HIV-1 transcription; HIV Tat-specific
                     factor 1"
                     /codon_start=1
                     /product="17S U2 SnRNP complex component HTATSF1"
                     /protein_id="NP_055315.2"
                     /db_xref="CCDS:CCDS14657.1"
                     /db_xref="GeneID:27336"
                     /db_xref="HGNC:HGNC:5276"
                     /db_xref="MIM:300346"
                     /translation="MSGTNLDGNDEFDEQLRMQELYGDGKDGDTQTDAGGEPDSLGQQ
                     PTDTPYEWDLDKKAWFPKITEDFIATYQANYGFSNDGASSSTANVEDVHARTAEEPPQ
                     EKAPEPTDARKKGEKRKAESGWFHVEEDRNTNVYVSGLPPDITVDEFIQLMSKFGIIM
                     RDPQTEEFKVKLYKDNQGNLKGDGLCCYLKRESVELALKLLDEDEIRGYKLHVEVAKF
                     QLKGEYDASKKKKKCKDYKKKLSMQQKQLDWRPERRAGPSRMRHERVVIIKNMFHPMD
                     FEDDPLVLNEIREDLRVECSKFGQIRKLLLFDRHPDGVASVSFRDPEEADYCIQTLDG
                     RWFGGRQITAQAWDGTTDYQVEETSREREERLRGWEAFLNAPEANRGLRRSDSVSASE
                     RAGPSRARHFSEHPSTSKMNAQETATGMAFEEPIDEKKFEKTEDGGEFEEGASENNAK
                     ESSPEKEAEEGCPEKESEEGCPKRGFEGSCSQKESEEGNPVRGSEEDSPKKESKKKTL
                     KNDCEENGLAKESEDDLNKESEEEVGPTKESEEDDSEKESDEDCSEKQSEDGSEREFE
                     ENGLEKDLDEEGSEKELHENVLDKELEENDSENSEFEDDGSEKVLDEEGSEREFDEDS
                     DEKEEEEDTYEKVFDDESDEKEDEEYADEKGLEAADKKAEEGDADEKLFEESDDKEDE
                     DADGKEVEDADEKLFEDDDSNEKLFDEEEDSSEKLFDDSDERGTLGGFGSVEEGPLST
                     GSSFILSSDDDDDDI"
     exon            280..460
                     /gene="HTATSF1"
                     /gene_synonym="dJ196E23.2; TAT-SF1; TATSF1"
                     /inference="alignment:Splign:2.1.0"
     exon            461..508
                     /gene="HTATSF1"
                     /gene_synonym="dJ196E23.2; TAT-SF1; TATSF1"
                     /inference="alignment:Splign:2.1.0"
     exon            509..663
                     /gene="HTATSF1"
                     /gene_synonym="dJ196E23.2; TAT-SF1; TATSF1"
                     /inference="alignment:Splign:2.1.0"
     exon            664..827
                     /gene="HTATSF1"
                     /gene_synonym="dJ196E23.2; TAT-SF1; TATSF1"
                     /inference="alignment:Splign:2.1.0"
     exon            828..927
                     /gene="HTATSF1"
                     /gene_synonym="dJ196E23.2; TAT-SF1; TATSF1"
                     /inference="alignment:Splign:2.1.0"
     exon            928..1017
                     /gene="HTATSF1"
                     /gene_synonym="dJ196E23.2; TAT-SF1; TATSF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1018..1155
                     /gene="HTATSF1"
                     /gene_synonym="dJ196E23.2; TAT-SF1; TATSF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1156..2694
                     /gene="HTATSF1"
                     /gene_synonym="dJ196E23.2; TAT-SF1; TATSF1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2659..2664
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HTATSF1"
                     /gene_synonym="dJ196E23.2; TAT-SF1; TATSF1"
                     /note="hexamer: AATAAA"
     polyA_site      2694
                     /gene="HTATSF1"
                     /gene_synonym="dJ196E23.2; TAT-SF1; TATSF1"
                     /note="major polyA site"
ORIGIN      
        1 agagcgcggt tgacctccct ttctctgctc agctccagcg tcatttcggc ctcttagttc
       61 ttctgaaccc tgctcctgag ctaggtagga aacatgagcg gcaccaactt ggatgggaac
      121 gatgagtttg atgagcagtt gcgaatgcaa gaattgtacg gagacggcaa ggatggtgac
      181 acccagaccg atgccggcgg agaacccgat tctctcgggc agcagccgac ggacactccc
      241 tacgagtggg acctggacaa aaaggcttgg ttccccaaga ttactgaaga tttcattgct
      301 acatatcagg ccaattatgg cttctctaac gatggcgcat ctagttctac cgcaaatgtt
      361 gaagatgtcc atgctaggac tgcagaggaa cctccacaag aaaaagcccc ggaacccact
      421 gatgccagaa agaagggaga aaaaagaaag gctgagtcag gatggtttca tgttgaagaa
      481 gacagaaata caaatgtata cgtgtctggt ttgcctccag atattacagt ggatgaattt
      541 atacaactta tgtccaagtt tggcattatt atgagagatc ctcagacaga agaatttaag
      601 gtcaaacttt acaaagataa tcaaggaaat cttaaaggag acggtctttg ctgttatttg
      661 aaaagagaat ctgtggaact tgcattaaaa cttttggatg aagatgaaat tagaggctac
      721 aaattacatg ttgaggtggc aaagtttcaa ctgaagggag aatatgatgc ctcaaagaag
      781 aagaagaagt gcaaagacta taagaagaag ctgtctatgc aacaaaagca gttggattgg
      841 agacctgaga ggcgagccgg accatcccgg atgcgccatg agcgagttgt catcatcaag
      901 aatatgtttc atcctatgga ttttgaggat gatccgttgg tgctgaatga gatcagagaa
      961 gaccttcgag tagagtgttc gaagtttgga caaattagga aactccttct ctttgatagg
     1021 cacccagatg gtgtggcctc tgtgtccttt cgggatccag aggaagctga ttattgtatt
     1081 cagactctcg atggaagatg gtttggtggc cgtcaaatca ctgcccaggc atgggatggg
     1141 actacagatt atcaggtgga ggaaacctca agagaaaggg aggaaaggct gagaggatgg
     1201 gaggctttcc tcaatgctcc tgaggccaac agaggcctta ggcgttcaga ttctgtctct
     1261 gcttccgaaa gggcagggcc ttctagagca aggcattttt cagagcaccc cagcacatct
     1321 aaaatgaatg ctcaagaaac tgcaactgga atggcgtttg aagaacctat agatgagaag
     1381 aagtttgaaa agacagaaga tgggggagaa tttgaagaag gtgcttctga aaacaatgct
     1441 aaggaaagta gccccgaaaa agaggctgaa gaaggctgcc ctgaaaaaga atctgaagag
     1501 ggctgcccca aaagagggtt tgaaggcagc tgctcccaaa aagagtctga agaaggcaat
     1561 cccgtaagag gatctgaaga ggatagtcct aaaaaagagt ctaaaaagaa gacactcaaa
     1621 aatgattgtg aagagaatgg ccttgcaaag gaatctgaag atgacctcaa caaggagtct
     1681 gaagaggagg ttggccccac aaaagagtcc gaagaagatg actcagagaa agagtctgat
     1741 gaagactgct ctgaaaaaca gtctgaagat ggctccgaaa gagaatttga agaaaatggt
     1801 ctcgagaaag atttggacga ggaaggttct gaaaaggagc ttcatgaaaa tgttcttgac
     1861 aaagagttag aagaaaatga ctctgaaaac tccgaatttg aagatgacgg ctctgaaaaa
     1921 gtgttagatg aggaaggctc tgagagagag tttgacgaag attcagatga aaaggaagaa
     1981 gaggaggata catatgaaaa agtatttgat gatgagtctg atgagaaaga ggatgaagaa
     2041 tatgcagatg aaaaggggct tgaagctgct gataaaaagg cggaagaagg tgatgcagat
     2101 gaaaagctgt ttgaagagtc agatgacaag gaagatgaag atgcagatgg aaaggaagtt
     2161 gaagatgctg acgaaaagtt gttcgaagat gatgattcca atgagaagtt gtttgatgag
     2221 gaggaagatt ccagtgagaa gttgtttgac gattctgatg agagggggac tttgggtggt
     2281 tttgggagtg ttgaagaagg gcccctatcc actggcagca gctttattct cagtagcgat
     2341 gatgatgacg atgatattta atcccttaaa cttgcttttt agggagagtc ctccatctac
     2401 atttgcctgt gcttcagggt aattactagt agtgttacat gaacatgtgc atagtggtag
     2461 gatgccatca gattaaagca ttgaagtgtt tcattgttac ctgtacctaa tggttttaaa
     2521 tatatgttaa ttgattgttt agttaaaatg tcatagttac aatgcaagta aactggatac
     2581 ttgttctttt gtcagatttg ttaaatgcat gcagaataat atttttaaga gtattgattg
     2641 aagtttgtga tattcatcaa taaaaatgag ttgataatat gcagaaactg aaaa
//
