LOCUS       NM_002157                557 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens heat shock protein family E (Hsp10) member 1 (HSPE1),
            mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_002157
VERSION     NM_002157.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   2  (bases 1 to 557)
  AUTHORS   Wang,J.C. and Chen,L.
  TITLE     Structural basis for the structural dynamics of human mitochondrial
            chaperonin mHsp60
  JOURNAL   Sci Rep 11 (1), 14809 (2021)
   PUBMED   34285302
  REMARK    GeneRIF: Structural basis for the structural dynamics of human
            mitochondrial chaperonin mHsp60.
            Erratum:[Sci Rep. 2021 Aug 24;11(1):17372. doi:
            10.1038/s41598-021-96945-w.. PMID: 34429499]
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 557)
  AUTHORS   Tang,Y., Yang,Y., Luo,J., Liu,S., Zhan,Y., Zang,H., Zheng,H.,
            Zhang,Y., Feng,J., Fan,S. and Wen,Q.
  TITLE     Overexpression of HSP10 correlates with HSP60 and Mcl-1 levels and
            predicts poor prognosis in non-small cell lung cancer patients
  JOURNAL   Cancer Biomark 30 (1), 85-94 (2021)
   PUBMED   32986659
  REMARK    GeneRIF: Overexpression of HSP10 correlates with HSP60 and Mcl-1
            levels and predicts poor prognosis in non-small cell lung cancer
            patients.
REFERENCE   4  (bases 1 to 557)
  AUTHORS   Gomez-Llorente,Y., Jebara,F., Patra,M., Malik,R., Nisemblat,S.,
            Chomsky-Hecht,O., Parnas,A., Azem,A., Hirsch,J.A. and
            Ubarretxena-Belandia,I.
  TITLE     Structural basis for active single and double ring complexes in
            human mitochondrial Hsp60-Hsp10 chaperonin
  JOURNAL   Nat Commun 11 (1), 1916 (2020)
   PUBMED   32317635
  REMARK    GeneRIF: structural basis for active single- and double-ring
            complexes coexisting in the mHsp60-mHsp10 chaperonin reaction cycle
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 557)
  AUTHORS   Hansen,J.J., Bross,P., Westergaard,M., Nielsen,M.N., Eiberg,H.,
            Borglum,A.D., Mogensen,J., Kristiansen,K., Bolund,L. and
            Gregersen,N.
  TITLE     Genomic structure of the human mitochondrial chaperonin genes:
            HSP60 and HSP10 are localised head to head on chromosome 2
            separated by a bidirectional promoter
  JOURNAL   Hum Genet 112 (1), 71-77 (2003)
   PUBMED   12483302
  REMARK    GeneRIF: The genome structure of this gene has been established.
REFERENCE   6  (bases 1 to 557)
  AUTHORS   Agarwal,S.K., Guru,S.C., Heppner,C., Erdos,M.R., Collins,R.M.,
            Park,S.Y., Saggar,S., Chandrasekharappa,S.C., Collins,F.S.,
            Spiegel,A.M., Marx,S.J. and Burns,A.L.
  TITLE     Menin interacts with the AP1 transcription factor JunD and
            represses JunD-activated transcription
  JOURNAL   Cell 96 (1), 143-152 (1999)
   PUBMED   9989505
REFERENCE   7  (bases 1 to 557)
  AUTHORS   Legname,G., Fossati,G., Gromo,G., Monzini,N., Marcucci,F. and
            Modena,D.
  TITLE     Expression in Escherichia coli, purification and functional
            activity of recombinant human chaperonin 10
  JOURNAL   FEBS Lett 361 (2-3), 211-214 (1995)
   PUBMED   7698325
REFERENCE   8  (bases 1 to 557)
  AUTHORS   Chen,J.J., McNealy,D.J., Dalal,S. and Androphy,E.J.
  TITLE     Isolation, sequence analysis and characterization of a cDNA
            encoding human chaperonin 10
  JOURNAL   Biochim Biophys Acta 1219 (1), 189-190 (1994)
   PUBMED   7916212
REFERENCE   9  (bases 1 to 557)
  AUTHORS   Monzini,N., Legname,G., Marcucci,F., Gromo,G. and Modena,D.
  TITLE     Identification and cloning of human chaperonin 10 homologue
  JOURNAL   Biochim Biophys Acta 1218 (3), 478-480 (1994)
   PUBMED   7914093
REFERENCE   10 (bases 1 to 557)
  AUTHORS   Cavanagh,A.C. and Morton,H.
  TITLE     The purification of early-pregnancy factor to homogeneity from
            human platelets and identification as chaperonin 10
  JOURNAL   Eur J Biochem 222 (2), 551-560 (1994)
   PUBMED   7912672
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC023518.2 and BG188453.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_002157.2.
            
            Summary: This gene encodes a major heat shock protein which
            functions as a chaperonin. Its structure consists of a heptameric
            ring which binds to another heat shock protein in order to form a
            symmetric, functional heterodimer which enhances protein folding in
            an ATP-dependent manner. This gene and its co-chaperonin, HSPD1,
            are arranged in a head-to-head orientation on chromosome 2.
            Naturally occurring read-through transcription occurs between this
            locus and the neighboring locus MOBKL3.[provided by RefSeq, Feb
            2011].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1163655.102397.1,
                                           SRR1163655.433354.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            MANE Ensembl match                 :: ENST00000233893.10/
                                                  ENSP00000233893.5
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-491               BC023518.2         57-547
            492-557             BG188453.1         435-500
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q33.1"
     gene            1..557
                     /gene="HSPE1"
                     /gene_synonym="CPN10; EPF; GROES; HSP10"
                     /note="heat shock protein family E (Hsp10) member 1"
                     /db_xref="GeneID:3336"
                     /db_xref="HGNC:HGNC:5269"
                     /db_xref="MIM:600141"
     exon            1..61
                     /gene="HSPE1"
                     /gene_synonym="CPN10; EPF; GROES; HSP10"
                     /inference="alignment:Splign:2.1.0"
     CDS             59..367
                     /gene="HSPE1"
                     /gene_synonym="CPN10; EPF; GROES; HSP10"
                     /note="heat shock 10kD protein 1 (chaperonin 10); 10 kDa
                     chaperonin; early-pregnancy factor; epididymis secretory
                     sperm binding protein; heat shock 10kDa protein 1; heat
                     shock protein family E member 1"
                     /codon_start=1
                     /product="10 kDa heat shock protein, mitochondrial"
                     /protein_id="NP_002148.1"
                     /db_xref="CCDS:CCDS2320.1"
                     /db_xref="GeneID:3336"
                     /db_xref="HGNC:HGNC:5269"
                     /db_xref="MIM:600141"
                     /translation="MAGQAFRKFLPLFDRVLVERSAAETVTKGGIMLPEKSQGKVLQA
                     TVVAVGSGSKGKGGEIQPVSVKVGDKVLLPEYGGTKVVLDDKDYFLFRDGDILGKYVD
                     "
     exon            62..226
                     /gene="HSPE1"
                     /gene_synonym="CPN10; EPF; GROES; HSP10"
                     /inference="alignment:Splign:2.1.0"
     exon            227..316
                     /gene="HSPE1"
                     /gene_synonym="CPN10; EPF; GROES; HSP10"
                     /inference="alignment:Splign:2.1.0"
     exon            317..557
                     /gene="HSPE1"
                     /gene_synonym="CPN10; EPF; GROES; HSP10"
                     /inference="alignment:Splign:2.1.0"
     regulatory      467..472
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HSPE1"
                     /gene_synonym="CPN10; EPF; GROES; HSP10"
                     /note="hexamer: AATAAA"
     polyA_site      491
                     /gene="HSPE1"
                     /gene_synonym="CPN10; EPF; GROES; HSP10"
                     /note="major polyA site"
     regulatory      539..544
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HSPE1"
                     /gene_synonym="CPN10; EPF; GROES; HSP10"
                     /note="hexamer: AATAAA"
     polyA_site      557
                     /gene="HSPE1"
                     /gene_synonym="CPN10; EPF; GROES; HSP10"
ORIGIN      
        1 gcgagtctct ttgcggcgct acactagagc agagtacgag tctgaggcgg agggagtaat
       61 ggcaggacaa gcgtttagaa agtttcttcc actctttgac cgagtattgg ttgaaaggag
      121 tgctgctgaa actgtaacca aaggaggcat tatgcttcca gaaaaatctc aaggaaaagt
      181 attgcaagca acagtagtcg ctgttggatc gggttctaaa ggaaagggtg gagagattca
      241 accagttagc gtgaaagttg gagataaagt tcttctccca gaatatggag gcaccaaagt
      301 agttctagat gacaaggatt atttcctatt tagagatggt gacattcttg gaaagtacgt
      361 agactgaaat aagtcactat tgaaatggca tcaacatgat gctgcccatt ccactgaagt
      421 tctgaaatct ttcgtcatgt aaataatttc catatttctc ttttataata aactaatgat
      481 aactaatgac atccagtgtc tccaaaattg tttccttgta ctgatataaa cacttccaaa
      541 taaaaatatg taaatga
//
