LOCUS       NM_006597               2264 bp    mRNA    linear   PRI 01-AUG-2024
DEFINITION  Homo sapiens heat shock protein family A (Hsp70) member 8 (HSPA8),
            transcript variant 1, mRNA.
ACCESSION   NM_006597
VERSION     NM_006597.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Chaari,A., Saikia,N., Paul,P., Yousef,M., Ding,F. and Ladjimi,M.
  TITLE     Experimental and computational investigation of the effect of Hsc70
            structural variants on inhibiting amylin aggregation
  JOURNAL   Biophys Chem 309, 107235 (2024)
   PUBMED   38608617
  REMARK    GeneRIF: Experimental and computational investigation of the effect
            of Hsc70 structural variants on inhibiting amylin aggregation.
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Sahu,W., Bai,T., Das,A., Mukherjee,S., Prusty,A., Mallick,N.R.,
            Elangovan,S. and Reddy,K.S.
  TITLE     Plasmodium falciparum J-dot localized J domain protein A8iJp
            modulates the chaperone activity of human HSPA8
  JOURNAL   FEBS Lett 598 (7), 818-836 (2024)
   PUBMED   38418371
  REMARK    GeneRIF: Plasmodium falciparum J-dot localized J domain protein
            A8iJp modulates the chaperone activity of human HSPA8.
REFERENCE   3  (bases 1 to 2264)
  AUTHORS   Kou,X., Yang,X., Zhao,Z. and Li,L.
  TITLE     HSPA8-mediated stability of the CLPP protein regulates
            mitochondrial autophagy in cisplatin-resistant ovarian cancer cells
  JOURNAL   Acta Biochim Biophys Sin (Shanghai) 56 (3), 356-365 (2024)
   PUBMED   38419499
  REMARK    GeneRIF: HSPA8-mediated stability of the CLPP protein regulates
            mitochondrial autophagy in cisplatin-resistant ovarian cancer
            cells.
REFERENCE   4  (bases 1 to 2264)
  AUTHORS   Zhang,C., Wang,Y., Wu,G., Sun,N., Bai,H., Li,X., Han,S., Zhou,H.,
            Qi,R. and Zhang,J.
  TITLE     RPL35A promotes the progression of cholangiocarcinoma by mediating
            HSPA8 ubiquitination
  JOURNAL   Biol Direct 19 (1), 16 (2024)
   PUBMED   38395908
  REMARK    GeneRIF: RPL35A promotes the progression of cholangiocarcinoma by
            mediating HSPA8 ubiquitination.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2264)
  AUTHORS   Rafiq,S., Mungure,I., Banz,Y., Niklaus,N.J., Kaufmann,T.,
            Muller,S., Jacquel,A., Robert,G., Auberger,P., Torbett,B.E.,
            Muller,S., Tschan,M.P. and Humbert,M.
  TITLE     HSPA8 Chaperone Complex Drives Chaperone-Mediated Autophagy
            Regulation in Acute Promyelocytic Leukemia Cell Differentiation
  JOURNAL   Pharmacology 109 (4), 216-230 (2024)
   PUBMED   38569476
  REMARK    GeneRIF: HSPA8 Chaperone Complex Drives Chaperone-Mediated
            Autophagy Regulation in Acute Promyelocytic Leukemia Cell
            Differentiation.
REFERENCE   6  (bases 1 to 2264)
  AUTHORS   Rasmussen,H.H., van Damme,J., Puype,M., Gesser,B., Celis,J.E. and
            Vandekerckhove,J.
  TITLE     Microsequences of 145 proteins recorded in the two-dimensional gel
            protein database of normal human epidermal keratinocytes
  JOURNAL   Electrophoresis 13 (12), 960-969 (1992)
   PUBMED   1286667
REFERENCE   7  (bases 1 to 2264)
  AUTHORS   Hattori,H., Liu,Y.C., Tohnai,I., Ueda,M., Kaneda,T., Kobayashi,T.,
            Tanabe,K. and Ohtsuka,K.
  TITLE     Intracellular localization and partial amino acid sequence of a
            stress-inducible 40-kDa protein in HeLa cells
  JOURNAL   Cell Struct Funct 17 (1), 77-86 (1992)
   PUBMED   1586970
REFERENCE   8  (bases 1 to 2264)
  AUTHORS   DeLuca-Flaherty,C., McKay,D.B., Parham,P. and Hill,B.L.
  TITLE     Uncoating protein (hsc70) binds a conformationally labile domain of
            clathrin light chain LCa to stimulate ATP hydrolysis
  JOURNAL   Cell 62 (5), 875-887 (1990)
   PUBMED   1975516
REFERENCE   9  (bases 1 to 2264)
  AUTHORS   Lim,M.Y., Davis,N., Zhang,J.Y. and Bose,H.R. Jr.
  TITLE     The v-rel oncogene product is complexed with cellular proteins
            including its proto-oncogene product and heat shock protein 70
  JOURNAL   Virology 175 (1), 149-160 (1990)
   PUBMED   2155506
REFERENCE   10 (bases 1 to 2264)
  AUTHORS   Chiang,H.L., Terlecky,S.R., Plant,C.P. and Dice,J.F.
  TITLE     A role for a 70-kilodalton heat shock protein in lysosomal
            degradation of intracellular proteins
  JOURNAL   Science 246 (4928), 382-385 (1989)
   PUBMED   2799391
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC395565.1, BC019816.2,
            AK222628.1 and AP000926.6.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_006597.5.
            
            Summary: This gene encodes a member of the heat shock protein 70
            family, which contains both heat-inducible and constitutively
            expressed members. This protein belongs to the latter group, which
            are also referred to as heat-shock cognate proteins. It functions
            as a chaperone, and binds to nascent polypeptides to facilitate
            correct folding. It also functions as an ATPase in the disassembly
            of clathrin-coated vesicles during transport of membrane components
            through the cell. Alternatively spliced transcript variants
            encoding different isoforms have been found for this gene.
            [provided by RefSeq, Aug 2011].
            
            Transcript Variant: This variant (1) represents the predominant
            transcript, and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.1011269.1,
                                           SRR3476690.494591.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2157437 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000534624.6/ ENSP00000432083.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-20                DC395565.1         133-152
            21-2256             BC019816.2         1-2236
            2257-2261           AK222628.1         1795-1799
            2262-2264           AP000926.6         35400-35402         c
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q24.1"
     gene            1..2264
                     /gene="HSPA8"
                     /gene_synonym="HEL-33; HEL-S-72p; HSC54; HSC70; HSC71;
                     HSP71; HSP73; HSPA10; LAP-1; LAP1; NIP71"
                     /note="heat shock protein family A (Hsp70) member 8"
                     /db_xref="GeneID:3312"
                     /db_xref="HGNC:HGNC:5241"
                     /db_xref="MIM:600816"
     exon            1..73
                     /gene="HSPA8"
                     /gene_synonym="HEL-33; HEL-S-72p; HSC54; HSC70; HSC71;
                     HSP71; HSP73; HSPA10; LAP-1; LAP1; NIP71"
                     /inference="alignment:Splign:2.1.0"
     exon            74..283
                     /gene="HSPA8"
                     /gene_synonym="HEL-33; HEL-S-72p; HSC54; HSC70; HSC71;
                     HSP71; HSP73; HSPA10; LAP-1; LAP1; NIP71"
                     /inference="alignment:Splign:2.1.0"
     CDS             79..2019
                     /gene="HSPA8"
                     /gene_synonym="HEL-33; HEL-S-72p; HSC54; HSC70; HSC71;
                     HSP71; HSP73; HSPA10; LAP-1; LAP1; NIP71"
                     /EC_number="3.6.4.10"
                     /note="isoform 1 is encoded by transcript variant 1; heat
                     shock 70kd protein 10; constitutive heat shock protein 70;
                     lipopolysaccharide-associated protein 1; LPS-associated
                     protein 1; heat shock cognate protein 54; heat shock
                     cognate 71 kDa protein; N-myristoyltransferase inhibitor
                     protein 71; epididymis luminal protein 33; epididymis
                     secretory sperm binding protein Li 72p; heat shock 70kDa
                     protein 8; heat shock protein family A member 8"
                     /codon_start=1
                     /product="heat shock cognate 71 kDa protein isoform 1"
                     /protein_id="NP_006588.1"
                     /db_xref="CCDS:CCDS8440.1"
                     /db_xref="GeneID:3312"
                     /db_xref="HGNC:HGNC:5241"
                     /db_xref="MIM:600816"
                     /translation="MSKGPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAF
                     TDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFDDAVVQSDMKHWPFMVVNDAGRPK
                     VQVEYKGETKSFYPEEVSSMVLTKMKEIAEAYLGKTVTNAVVTVPAYFNDSQRQATKD
                     AGTIAGLNVLRIINEPTAAAIAYGLDKKVGAERNVLIFDLGGGTFDVSILTIEDGIFE
                     VKSTAGDTHLGGEDFDNRMVNHFIAEFKRKHKKDISENKRAVRRLRTACERAKRTLSS
                     STQASIEIDSLYEGIDFYTSITRARFEELNADLFRGTLDPVEKALRDAKLDKSQIHDI
                     VLVGGSTRIPKIQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAILSGDKSENVQDLL
                     LLDVTPLSLGIETAGGVMTVLIKRNTTIPTKQTQTFTTYSDNQPGVLIQVYEGERAMT
                     KDNNLLGKFELTGIPPAPRGVPQIEVTFDIDANGILNVSAVDKSTGKENKITITNDKG
                     RLSKEDIERMVQEAEKYKAEDEKQRDKVSSKNSLESYAFNMKATVEDEKLQGKINDED
                     KQKILDKCNEIINWLDKNQTAEKEEFEHQQKELEKVCNPIITKLYQSAGGMPGGMPGG
                     FPGGGAPPSGGASSGPTIEEVD"
     exon            284..489
                     /gene="HSPA8"
                     /gene_synonym="HEL-33; HEL-S-72p; HSC54; HSC70; HSC71;
                     HSP71; HSP73; HSPA10; LAP-1; LAP1; NIP71"
                     /inference="alignment:Splign:2.1.0"
     exon            490..642
                     /gene="HSPA8"
                     /gene_synonym="HEL-33; HEL-S-72p; HSC54; HSC70; HSC71;
                     HSP71; HSP73; HSPA10; LAP-1; LAP1; NIP71"
                     /inference="alignment:Splign:2.1.0"
     exon            643..1198
                     /gene="HSPA8"
                     /gene_synonym="HEL-33; HEL-S-72p; HSC54; HSC70; HSC71;
                     HSP71; HSP73; HSPA10; LAP-1; LAP1; NIP71"
                     /inference="alignment:Splign:2.1.0"
     exon            1199..1401
                     /gene="HSPA8"
                     /gene_synonym="HEL-33; HEL-S-72p; HSC54; HSC70; HSC71;
                     HSP71; HSP73; HSPA10; LAP-1; LAP1; NIP71"
                     /inference="alignment:Splign:2.1.0"
     exon            1402..1600
                     /gene="HSPA8"
                     /gene_synonym="HEL-33; HEL-S-72p; HSC54; HSC70; HSC71;
                     HSP71; HSP73; HSPA10; LAP-1; LAP1; NIP71"
                     /inference="alignment:Splign:2.1.0"
     exon            1601..1833
                     /gene="HSPA8"
                     /gene_synonym="HEL-33; HEL-S-72p; HSC54; HSC70; HSC71;
                     HSP71; HSP73; HSPA10; LAP-1; LAP1; NIP71"
                     /inference="alignment:Splign:2.1.0"
     exon            1834..2264
                     /gene="HSPA8"
                     /gene_synonym="HEL-33; HEL-S-72p; HSC54; HSC70; HSC71;
                     HSP71; HSP73; HSPA10; LAP-1; LAP1; NIP71"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2234..2239
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HSPA8"
                     /gene_synonym="HEL-33; HEL-S-72p; HSC54; HSC70; HSC71;
                     HSP71; HSP73; HSPA10; LAP-1; LAP1; NIP71"
                     /note="hexamer: AATAAA"
     polyA_site      2264
                     /gene="HSPA8"
                     /gene_synonym="HEL-33; HEL-S-72p; HSC54; HSC70; HSC71;
                     HSP71; HSP73; HSPA10; LAP-1; LAP1; NIP71"
                     /note="major polyA site"
ORIGIN      
        1 ctcattgaac tcgcctgcag ctcttgggtt ttttgtggct tccttcgtta ttggagccag
       61 gcctacaccc cagcaaccat gtccaaggga cctgcagttg gtattgatct tggcaccacc
      121 tactcttgtg tgggtgtttt ccagcacgga aaagtcgaga taattgccaa tgatcaggga
      181 aaccgaacca ctccaagcta tgtcgccttt acggacactg aacggttgat cggtgatgcc
      241 gcaaagaatc aagttgcaat gaaccccacc aacacagttt ttgatgccaa acgtctgatt
      301 ggacgcagat ttgatgatgc tgttgtccag tctgatatga aacattggcc ctttatggtg
      361 gtgaatgatg ctggcaggcc caaggtccaa gtagaataca agggagagac caaaagcttc
      421 tatccagagg aggtgtcttc tatggttctg acaaagatga aggaaattgc agaagcctac
      481 cttgggaaga ctgttaccaa tgctgtggtc acagtgccag cttactttaa tgactctcag
      541 cgtcaggcta ccaaagatgc tggaactatt gctggtctca atgtacttag aattattaat
      601 gagccaactg ctgctgctat tgcttacggc ttagacaaaa aggttggagc agaaagaaac
      661 gtgctcatct ttgacctggg aggtggcact tttgatgtgt caatcctcac tattgaggat
      721 ggaatctttg aggtcaagtc tacagctgga gacacccact tgggtggaga agattttgac
      781 aaccgaatgg tcaaccattt tattgctgag tttaagcgca agcataagaa ggacatcagt
      841 gagaacaaga gagctgtaag acgcctccgt actgcttgtg aacgtgctaa gcgtaccctc
      901 tcttccagca cccaggccag tattgagatc gattctctct atgaaggaat cgacttctat
      961 acctccatta cccgtgcccg atttgaagaa ctgaatgctg acctgttccg tggcaccctg
     1021 gacccagtag agaaagccct tcgagatgcc aaactagaca agtcacagat tcatgatatt
     1081 gtcctggttg gtggttctac tcgtatcccc aagattcaga agcttctcca agacttcttc
     1141 aatggaaaag aactgaataa gagcatcaac cctgatgaag ctgttgctta tggtgcagct
     1201 gtccaggcag ccatcttgtc tggagacaag tctgagaatg ttcaagattt gctgctcttg
     1261 gatgtcactc ctctttccct tggtattgaa actgctggtg gagtcatgac tgtcctcatc
     1321 aagcgtaata ccaccattcc taccaagcag acacagacct tcactaccta ttctgacaac
     1381 cagcctggtg tgcttattca ggtttatgaa ggcgagcgtg ccatgacaaa ggataacaac
     1441 ctgcttggca agtttgaact cacaggcata cctcctgcac cccgaggtgt tcctcagatt
     1501 gaagtcactt ttgacattga tgccaatggt atactcaatg tctctgctgt ggacaagagt
     1561 acgggaaaag agaacaagat tactatcact aatgacaagg gccgtttgag caaggaagac
     1621 attgaacgta tggtccagga agctgagaag tacaaagctg aagatgagaa gcagagggac
     1681 aaggtgtcat ccaagaattc acttgagtcc tatgccttca acatgaaagc aactgttgaa
     1741 gatgagaaac ttcaaggcaa gattaacgat gaggacaaac agaagattct ggacaagtgt
     1801 aatgaaatta tcaactggct tgataagaat cagactgctg agaaggaaga atttgaacat
     1861 caacagaaag agctggagaa agtttgcaac cccatcatca ccaagctgta ccagagtgca
     1921 ggaggcatgc caggaggaat gcctggggga tttcctggtg gtggagctcc tccctctggt
     1981 ggtgcttcct cagggcccac cattgaagag gttgattaag ccaaccaagt gtagatgtag
     2041 cattgttcca cacatttaaa acatttgaag gacctaaatt cgtagcaaat tctgtggcag
     2101 ttttaaaaag ttaagctgct atagtaagtt actgggcatt ctcaatactt gaatatggaa
     2161 catatgcaca ggggaaggaa ataacattgc actttataaa cactgtattg taagtggaaa
     2221 atgcaatgtc ttaaataaaa ctatttaaaa ttggcaccat acaa
//
