LOCUS       NM_005347               3921 bp    mRNA    linear   PRI 15-OCT-2024
DEFINITION  Homo sapiens heat shock protein family A (Hsp70) member 5 (HSPA5),
            mRNA.
ACCESSION   NM_005347
VERSION     NM_005347.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Mafi,S., Dehghani,M., Khalvati,B., Abidi,H., Ghorbani,M.,
            Jalali,P., Whichelo,R., Salehi,Z., Markowska,A., Reyes,A.,
            Pecic,S., Los,M.J., Ghavami,S. and Nikseresht,M.
  TITLE     Targeting PERK and GRP78 in colorectal cancer: Genetic insights and
            novel therapeutic approaches
  JOURNAL   Eur J Pharmacol 982, 176899 (2024)
   PUBMED   39153651
  REMARK    GeneRIF: Targeting PERK and GRP78 in colorectal cancer: Genetic
            insights and novel therapeutic approaches.
REFERENCE   2  (bases 1 to 3921)
  AUTHORS   He,J., Xu,M., Chen,Y. and Wu,S.
  TITLE     Grp78 regulates NLRP3 inflammasome and participates in Sjogren's
            syndrome
  JOURNAL   Int Immunopharmacol 140, 112815 (2024)
   PUBMED   39088921
  REMARK    GeneRIF: Grp78 regulates NLRP3 inflammasome and participates in
            Sjogren's syndrome.
REFERENCE   3  (bases 1 to 3921)
  AUTHORS   Zhao,J., Kang,M., Li,H., Rong,L., Wang,Y., Xue,Y., Yao,Y. and
            Fang,Y.
  TITLE     QRICH1 suppresses pediatric T-cell acute lymphoblastic leukemia by
            inhibiting GRP78
  JOURNAL   Cell Death Dis 15 (9), 646 (2024)
   PUBMED   39227586
  REMARK    GeneRIF: QRICH1 suppresses pediatric T-cell acute lymphoblastic
            leukemia by inhibiting GRP78.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3921)
  AUTHORS   Hrabos,D., Poggiolini,I., Civitelli,L., Galli,E., Esapa,C.,
            Saarma,M., Lindholm,P. and Parkkinen,L.
  TITLE     Unfolded protein response markers Grp78 and eIF2alpha are
            upregulated with increasing alpha-synuclein levels in Lewy body
            disease
  JOURNAL   Neuropathol Appl Neurobiol 50 (4), e12999 (2024)
   PUBMED   39036837
  REMARK    GeneRIF: Unfolded protein response markers Grp78 and eIF2alpha are
            upregulated with increasing alpha-synuclein levels in Lewy body
            disease.
REFERENCE   5  (bases 1 to 3921)
  AUTHORS   Hu,J., Cui,Z., Huang,K., Su,R. and Zhao,S.
  TITLE     Role of the GRP78-c-Src signaling pathway on osteoblast
            differentiation of periodontal ligament fibroblasts induced by
            cyclic mechanical stretch
  JOURNAL   Hua Xi Kou Qiang Yi Xue Za Zhi 42 (3), 304-312 (2024)
   PUBMED   39049649
  REMARK    GeneRIF: Role of the GRP78-c-Src signaling pathway on osteoblast
            differentiation of periodontal ligament fibroblasts induced by
            cyclic mechanical stretch.
REFERENCE   6  (bases 1 to 3921)
  AUTHORS   Chao,C.C. and Lin-Chao,S.
  TITLE     A direct-repeat sequence of the human BiP gene is required for
            A23187-mediated inducibility and an inducible nuclear factor
            binding
  JOURNAL   Nucleic Acids Res 20 (24), 6481-6485 (1992)
   PUBMED   1480470
REFERENCE   7  (bases 1 to 3921)
  AUTHORS   Dawson,S.J. and White,L.A.
  TITLE     Treatment of Haemophilus aphrophilus endocarditis with
            ciprofloxacin
  JOURNAL   J Infect 24 (3), 317-320 (1992)
   PUBMED   1602151
REFERENCE   8  (bases 1 to 3921)
  AUTHORS   Suzuki,C.K., Bonifacino,J.S., Lin,A.Y., Davis,M.M. and
            Klausner,R.D.
  TITLE     Regulating the retention of T-cell receptor alpha chain variants
            within the endoplasmic reticulum: Ca(2+)-dependent association with
            BiP
  JOURNAL   J Cell Biol 114 (2), 189-205 (1991)
   PUBMED   1649196
REFERENCE   9  (bases 1 to 3921)
  AUTHORS   Dana,R.C., Welch,W.J. and Deftos,L.J.
  TITLE     Heat shock proteins bind calcitonin
  JOURNAL   Endocrinology 126 (1), 672-674 (1990)
   PUBMED   2294010
REFERENCE   10 (bases 1 to 3921)
  AUTHORS   Ting,J. and Lee,A.S.
  TITLE     Human gene encoding the 78,000-dalton glucose-regulated protein and
            its pseudogene: structure, conservation, and regulation
  JOURNAL   DNA 7 (4), 275-286 (1988)
   PUBMED   2840249
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA549454.1, BC020235.1 and
            X87949.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_005347.4.
            
            Summary: The protein encoded by this gene is a member of the heat
            shock protein 70 (HSP70) family. This protein localizes to the
            lumen of the endoplasmic reticulum (ER) where it operates as a
            typical HSP70 chaperone involved in the folding and assembly of
            proteins in the ER and is a master regulator of ER homeostasis.
            During cellular stress, as during viral infection or tumorogenesis,
            this protein interacts with the transmembrane stress sensor
            proteins PERK (protein kinase R-like endoplasmic reticulum kinase),
            IRE1 (inositol-requiring kinase 1), and ATF6 (activating
            transcription factor 6) where it acts as a repressor of the
            unfolded protein response (UPR) and also plays a role in cellular
            apoptosis and senescence. Elevated expression and atypical
            translocation of this protein to the cell surface has been reported
            in viral infections and some types of cancer cells. At the cell
            surface this protein may facilitate viral attachment and entry to
            host cells. This gene is a therapeutic target for the treatment of
            coronavirus diseases and chemoresistant cancers. [provided by
            RefSeq, Jul 2020].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC020235.1, SRR11853565.29163.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA2142348
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            coronavirus related    :: relevant for COVID-19 treatment
            MANE Ensembl match     :: ENST00000324460.7/ ENSP00000324173.6
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-220               DA549454.1         151-370
            221-2401            BC020235.1         208-2388
            2402-2535           X87949.1           2404-2537
            2536-2691           BC020235.1         2523-2678
            2692-3921           BC020235.1         2680-3909
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9q33.3"
     gene            1..3921
                     /gene="HSPA5"
                     /gene_synonym="BIP; GRP78; HEL-S-89n"
                     /note="heat shock protein family A (Hsp70) member 5"
                     /db_xref="GeneID:3309"
                     /db_xref="HGNC:HGNC:5238"
                     /db_xref="MIM:138120"
     exon            1..339
                     /gene="HSPA5"
                     /gene_synonym="BIP; GRP78; HEL-S-89n"
                     /inference="alignment:Splign:2.1.0"
     CDS             218..2182
                     /gene="HSPA5"
                     /gene_synonym="BIP; GRP78; HEL-S-89n"
                     /EC_number="3.6.4.10"
                     /note="78 kDa glucose-regulated protein; immunoglobulin
                     heavy chain-binding protein; endoplasmic reticulum lumenal
                     Ca(2+)-binding protein grp78; HSP70 family protein 5;
                     epididymis secretory sperm binding protein Li 89n; heat
                     shock 70kDa protein 5 (glucose-regulated protein, 78kDa);
                     binding-immunoglobulin protein; heat shock protein family
                     A member 5; heat shock protein 70 family protein 5;
                     endoplasmic reticulum chaperone BiP"
                     /codon_start=1
                     /product="endoplasmic reticulum chaperone BiP precursor"
                     /protein_id="NP_005338.1"
                     /db_xref="CCDS:CCDS6863.1"
                     /db_xref="GeneID:3309"
                     /db_xref="HGNC:HGNC:5238"
                     /db_xref="MIM:138120"
                     /translation="MKLSLVAAMLLLLSAARAEEEDKKEDVGTVVGIDLGTTYSCVGV
                     FKNGRVEIIANDQGNRITPSYVAFTPEGERLIGDAAKNQLTSNPENTVFDAKRLIGRT
                     WNDPSVQQDIKFLPFKVVEKKTKPYIQVDIGGGQTKTFAPEEISAMVLTKMKETAEAY
                     LGKKVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVMRIINEPTAAAIAYGLDKREGEK
                     NILVFDLGGGTFDVSLLTIDNGVFEVVATNGDTHLGGEDFDQRVMEHFIKLYKKKTGK
                     DVRKDNRAVQKLRREVEKAKRALSSQHQARIEIESFYEGEDFSETLTRAKFEELNMDL
                     FRSTMKPVQKVLEDSDLKKSDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDE
                     AVAYGAAVQAGVLSGDQDTGDLVLLDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQI
                     FSTASDNQPTVTIKVYEGERPLTKDNHLLGTFDLTGIPPAPRGVPQIEVTFEIDVNGI
                     LRVTAEDKGTGNKNKITITNDQNRLTPEEIERMVNDAEKFAEEDKKLKERIDTRNELE
                     SYAYSLKNQIGDKEKLGGKLSSEDKETMEKAVEEKIEWLESHQDADIEDFKAKKKELE
                     EIVQPIISKLYGSAGPPPTGEEDTAEKDEL"
     exon            340..571
                     /gene="HSPA5"
                     /gene_synonym="BIP; GRP78; HEL-S-89n"
                     /inference="alignment:Splign:2.1.0"
     exon            572..709
                     /gene="HSPA5"
                     /gene_synonym="BIP; GRP78; HEL-S-89n"
                     /inference="alignment:Splign:2.1.0"
     exon            710..822
                     /gene="HSPA5"
                     /gene_synonym="BIP; GRP78; HEL-S-89n"
                     /inference="alignment:Splign:2.1.0"
     exon            823..1213
                     /gene="HSPA5"
                     /gene_synonym="BIP; GRP78; HEL-S-89n"
                     /inference="alignment:Splign:2.1.0"
     exon            1214..1451
                     /gene="HSPA5"
                     /gene_synonym="BIP; GRP78; HEL-S-89n"
                     /inference="alignment:Splign:2.1.0"
     exon            1452..1619
                     /gene="HSPA5"
                     /gene_synonym="BIP; GRP78; HEL-S-89n"
                     /inference="alignment:Splign:2.1.0"
     exon            1620..3921
                     /gene="HSPA5"
                     /gene_synonym="BIP; GRP78; HEL-S-89n"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2509..2514
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HSPA5"
                     /gene_synonym="BIP; GRP78; HEL-S-89n"
                     /note="hexamer: AATAAA"
     polyA_site      2535
                     /gene="HSPA5"
                     /gene_synonym="BIP; GRP78; HEL-S-89n"
                     /note="major polyA site"
     regulatory      3902..3907
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HSPA5"
                     /gene_synonym="BIP; GRP78; HEL-S-89n"
                     /note="hexamer: ATTAAA"
     polyA_site      3921
                     /gene="HSPA5"
                     /gene_synonym="BIP; GRP78; HEL-S-89n"
ORIGIN      
        1 gacgccggcc aagacagcac agacagattg acctattggg gtgtttcgcg agtgtgagag
       61 ggaagcgccg cggcctgtat ttctagacct gcccttcgcc tggttcgtgg cgccttgtga
      121 ccccgggccc ctgccgcctg caagtcggaa attgcgctgt gctcctgtgc tacggcctgt
      181 ggctggactg cctgctgctg cccaactggc tggcaagatg aagctctccc tggtggccgc
      241 gatgctgctg ctgctcagcg cggcgcgggc cgaggaggag gacaagaagg aggacgtggg
      301 cacggtggtc ggcatcgacc tggggaccac ctactcctgc gtcggcgtgt tcaagaacgg
      361 ccgcgtggag atcatcgcca acgatcaggg caaccgcatc acgccgtcct atgtcgcctt
      421 cactcctgaa ggggaacgtc tgattggcga tgccgccaag aaccagctca cctccaaccc
      481 cgagaacacg gtctttgacg ccaagcggct catcggccgc acgtggaatg acccgtctgt
      541 gcagcaggac atcaagttct tgccgttcaa ggtggttgaa aagaaaacta aaccatacat
      601 tcaagttgat attggaggtg ggcaaacaaa gacatttgct cctgaagaaa tttctgccat
      661 ggttctcact aaaatgaaag aaaccgctga ggcttatttg ggaaagaagg ttacccatgc
      721 agttgttact gtaccagcct attttaatga tgcccaacgc caagcaacca aagacgctgg
      781 aactattgct ggcctaaatg ttatgaggat catcaacgag cctacggcag ctgctattgc
      841 ttatggcctg gataagaggg agggggagaa gaacatcctg gtgtttgacc tgggtggcgg
      901 aaccttcgat gtgtctcttc tcaccattga caatggtgtc ttcgaagttg tggccactaa
      961 tggagatact catctgggtg gagaagactt tgaccagcgt gtcatggaac acttcatcaa
     1021 actgtacaaa aagaagacgg gcaaagatgt caggaaagac aatagagctg tgcagaaact
     1081 ccggcgcgag gtagaaaagg ccaaacgggc cctgtcttct cagcatcaag caagaattga
     1141 aattgagtcc ttctatgaag gagaagactt ttctgagacc ctgactcggg ccaaatttga
     1201 agagctcaac atggatctgt tccggtctac tatgaagccc gtccagaaag tgttggaaga
     1261 ttctgatttg aagaagtctg atattgatga aattgttctt gttggtggct cgactcgaat
     1321 tccaaagatt cagcaactgg ttaaagagtt cttcaatggc aaggaaccat cccgtggcat
     1381 aaacccagat gaagctgtag cgtatggtgc tgctgtccag gctggtgtgc tctctggtga
     1441 tcaagataca ggtgacctgg tactgcttga tgtatgtccc cttacacttg gtattgaaac
     1501 tgtgggaggt gtcatgacca aactgattcc aaggaacaca gtggtgccta ccaagaagtc
     1561 tcagatcttt tctacagctt ctgataatca accaactgtt acaatcaagg tctatgaagg
     1621 tgaaagaccc ctgacaaaag acaatcatct tctgggtaca tttgatctga ctggaattcc
     1681 tcctgctcct cgtggggtcc cacagattga agtcaccttt gagatagatg tgaatggtat
     1741 tcttcgagtg acagctgaag acaagggtac agggaacaaa aataagatca caatcaccaa
     1801 tgaccagaat cgcctgacac ctgaagaaat cgaaaggatg gttaatgatg ctgagaagtt
     1861 tgctgaggaa gacaaaaagc tcaaggagcg cattgatact agaaatgagt tggaaagcta
     1921 tgcctattct ctaaagaatc agattggaga taaagaaaag ctgggaggta aactttcctc
     1981 tgaagataag gagaccatgg aaaaagctgt agaagaaaag attgaatggc tggaaagcca
     2041 ccaagatgct gacattgaag acttcaaagc taagaagaag gaactggaag aaattgttca
     2101 accaattatc agcaaactct atggaagtgc aggccctccc ccaactggtg aagaggatac
     2161 agcagaaaaa gatgagttgt agacactgat ctgctagtgc tgtaatattg taaatactgg
     2221 actcaggaac ttttgttagg aaaaaattga aagaacttaa gtctcgaatg taattggaat
     2281 cttcacctca gagtggagtt gaaactgcta tagcctaagc ggctgtttac tgcttttcat
     2341 tagcagttgc tcacatgtct ttgggtgggg gggagaagaa gaattggcca tcttaaaaag
     2401 cgggtaaaaa acctgggtta gggtgtgtgt tcaccttcaa aatgttctat ttaacaactg
     2461 ggtcatgtgc atctggtgta ggaagttttt tctaccataa gtgacaccaa taaatgtttg
     2521 ttatttacac tggtctaatg tttgtgagaa gcttctaatt agatcaatta cttattttag
     2581 gaaatttaag actagatact cgtgtgtggg gtgaggggag ggagtatttg gtatgttggg
     2641 ataaggaaac acttctattt aatgcttcca gggatttttt tttttttttt taaccctcct
     2701 gggcccaagt gatccttcca cctcagtctc ccagctaatt gagaccacag gcttgttacc
     2761 accatgctcg gcttttgcat taatctaaga aaaggggaga gaagttaatc cacatcttta
     2821 ctcaggcaag gggcatttca cagtgcccaa gagtggggtt ttcttgaaca tacttggttt
     2881 cctatttccc cttatctttc taaaactgcc tttctggtgg ctttttttaa aattattact
     2941 aatgatgctt ttatagctgc ttggattctc tgagaaatga tggggagtga gtgatcactg
     3001 gtattaactt tatacacttg gatttcattt gtaactttag gatgtaaagg tatattgtga
     3061 accctagctg tgtcagaatc tccatccctg aaatttctca ttagtggtac tggggtggga
     3121 tcttggatgg tgacattgaa actacactaa atcccctcac tatgaatggg ttgttaaagg
     3181 caatggtttg tgtcaaaact ggtttaggat tacttagatt gtgttcctga agaaaagagt
     3241 ccaggtaaat ggtatgatca ataaaggaca ggctggtgct aacataaaat ccaatattgt
     3301 aatcctagca ctttgggagg ccaaggcggg tggatcacaa ggtcaagaga tagagaccat
     3361 ctttgccaac atggtgaaac tccatctcta ctgaaaatac aaaaattagc tgggcgtggt
     3421 agtgcaagct gaaggctgag gcaggagaat cactcgaacc cgggaggcag aggttgcagt
     3481 gagccgagat cacaccactg tactccagcc cggcactcca gcctggcgac aagagtgaga
     3541 ctccacctca aaaaaaaaaa aaagaatcca atactgccca aggataggta ttttatagat
     3601 gggcaactgg ctgaaaggtt aattctctag ggctagtaga actggatccc aacaccaaac
     3661 tcttaattag acctaggcct cagctgcact gcccgaaaag catttgggca gaccctgagc
     3721 agaatactgg tctcaggcca agcccaatac agccattaaa gatgacctac agtgctgtgt
     3781 accctggggc aatagggtta aatggtagtt agcaactagg gctagtcttc ccttacctca
     3841 aaggctctca ctaccgtgga ccacctagtc tgtaactctt tctgaggagc tgttactgaa
     3901 tattaaaaag atagacttca a
//
