LOCUS       NM_014278              10608 bp    mRNA    linear   PRI 16-JUN-2024
DEFINITION  Homo sapiens heat shock protein family A (Hsp70) member 4 like
            (HSPA4L), transcript variant 1, mRNA.
ACCESSION   NM_014278
VERSION     NM_014278.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10608)
  AUTHORS   Zhu,M., Huang,Y., Tang,J., Shao,S., Zhang,L., Zhou,Y., He,S. and
            Wang,Y.
  TITLE     Role of Apg-1 in HSF1 activation and bortezomib sensitivity in
            myeloma cells
  JOURNAL   Exp Hematol 81, 50-59 (2020)
   PUBMED   31899217
  REMARK    GeneRIF: Apg-1 knockdown sensitized myeloma cells to bortezomib
            treatment, which may provide a new approach in multiple myeloma
            treatment.
REFERENCE   2  (bases 1 to 10608)
  AUTHORS   Liu,X., Wang,X. and Liu,F.
  TITLE     Decreased expression of heat shock protein A4L in spermatozoa is
            positively related to poor human sperm quality
  JOURNAL   Mol Reprod Dev 86 (4), 379-386 (2019)
   PUBMED   30637842
  REMARK    GeneRIF: decreased expression of HSPA4L in immature and
            asthenozoospermic spermatozoa was validated by western blot
            analysis. Functional analysis revealed a correlation between HSPA4L
            and sperm motility by Spearman correlation analysis and its
            involvement in sperm-oocyte penetration by the human sperm-hamster
            egg penetration test.
REFERENCE   3  (bases 1 to 10608)
  AUTHORS   Wang,S., Mo,Y., Midorikawa,K., Zhang,Z., Huang,G., Ma,N., Zhao,W.,
            Hiraku,Y., Oikawa,S. and Murata,M.
  TITLE     The potent tumor suppressor miR-497 inhibits cancer phenotypes in
            nasopharyngeal carcinoma by targeting ANLN and HSPA4L
  JOURNAL   Oncotarget 6 (34), 35893-35907 (2015)
   PUBMED   26486082
  REMARK    GeneRIF: Findings indicate that miR-497 is a potent tumor
            suppressor that inhibits cancer phenotypes by targeting ANLN and
            HSPA4L in NPC.
REFERENCE   4  (bases 1 to 10608)
  AUTHORS   Kuo,Y., Ren,S., Lao,U., Edgar,B.A. and Wang,T.
  TITLE     Suppression of polyglutamine protein toxicity by co-expression of a
            heat-shock protein 40 and a heat-shock protein 110
  JOURNAL   Cell Death Dis 4 (10), e833 (2013)
   PUBMED   24091676
  REMARK    GeneRIF: HSP40 and HSP110 function together in protein homeostasis
            control.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 10608)
  AUTHORS   Song,Y., Nagy,M., Ni,W., Tyagi,N.K., Fenton,W.A.,
            Lopez-Giraldez,F., Overton,J.D., Horwich,A.L. and Brady,S.T.
  TITLE     Molecular chaperone Hsp110 rescues a vesicle transport defect
            produced by an ALS-associated mutant SOD1 protein in squid axoplasm
  JOURNAL   Proc Natl Acad Sci U S A 110 (14), 5428-5433 (2013)
   PUBMED   23509252
  REMARK    GeneRIF: Human molecular chaperone Hsp110 rescues a vesicle
            transport defect produced by an ALS-associated mutant SOD1 protein
            in squid axoplasm.
REFERENCE   6  (bases 1 to 10608)
  AUTHORS   Mala,J.G. and Takeuchi,S.
  TITLE     Molecular cloning of OSP94: A significant biomarker protein of
            hypertensive human heart and a member of HSP110 family
  JOURNAL   Mol Biotechnol 42 (2), 175-194 (2009)
   PUBMED   19169850
REFERENCE   7  (bases 1 to 10608)
  AUTHORS   Takahashi,H., Furukawa,T., Yano,T., Sato,N., Takizawa,J.,
            Kurasaki,T., Abe,T., Narita,M., Masuko,M., Koyama,S., Toba,K.,
            Takahashi,M. and Aizawa,Y.
  TITLE     Identification of an overexpressed gene, HSPA4L, the product of
            which can provoke prevalent humoral immune responses in leukemia
            patients
  JOURNAL   Exp Hematol 35 (7), 1091-1099 (2007)
   PUBMED   17588478
REFERENCE   8  (bases 1 to 10608)
  AUTHORS   Colgan,S.P., Pitman,R.S., Nagaishi,T., Mizoguchi,A., Mizoguchi,E.,
            Mayer,L.F., Shao,L., Sartor,R.B., Subjeck,J.R. and Blumberg,R.S.
  TITLE     Intestinal heat shock protein 110 regulates expression of CD1d on
            intestinal epithelial cells
  JOURNAL   J Clin Invest 112 (5), 745-754 (2003)
   PUBMED   12952923
  REMARK    GeneRIF: a novel autocrine pathway of CD1d regulation by Hsp110.
REFERENCE   9  (bases 1 to 10608)
  AUTHORS   Nonoguchi,K., Tokuchi,H., Okuno,H., Watanabe,H., Egawa,H.,
            Saito,K., Ogawa,O. and Fujita,J.
  TITLE     Expression of Apg-1, a member of the Hsp110 family, in the human
            testis and sperm
  JOURNAL   Int J Urol 8 (6), 308-314 (2001)
   PUBMED   11389747
REFERENCE   10 (bases 1 to 10608)
  AUTHORS   Nonoguchi,K., Itoh,K., Xue,J.H., Tokuchi,H., Nishiyama,H.,
            Kaneko,Y., Tatsumi,K., Okuno,H., Tomiwa,K. and Fujita,J.
  TITLE     Cloning of human cDNAs for Apg-1 and Apg-2, members of the Hsp110
            family, and chromosomal assignment of their genes
  JOURNAL   Gene 237 (1), 21-28 (1999)
   PUBMED   10524232
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC040560.1, AC093591.3 and
            BM679019.1.
            
            On Nov 22, 2018 this sequence version replaced NM_014278.3.
            
            Summary: The protein encoded by this gene is heat shock inducible
            and may act as a chaperone. The encoded protein can protect the
            heat-shocked cell against the harmful effects of aggregated
            proteins. This gene is highly expressed in leukemia cells and may
            be a good target for therapeutic intervention. Several transcripts
            encoding different isoforms have been found for this gene.
            [provided by RefSeq, Dec 2015].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660807.69021.1,
                                           SRR1660803.246850.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1968832
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000296464.9/ ENSP00000296464.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3242              BC040560.1         25-3266
            3243-3243           AC093591.3         137070-137070
            3244-3948           BM679019.1         17-721              c
            3949-10608          AC093591.3         137776-144435
FEATURES             Location/Qualifiers
     source          1..10608
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q28.1"
     gene            1..10608
                     /gene="HSPA4L"
                     /gene_synonym="APG-1; APG1; HSPH3; Osp94"
                     /note="heat shock protein family A (Hsp70) member 4 like"
                     /db_xref="GeneID:22824"
                     /db_xref="HGNC:HGNC:17041"
                     /db_xref="MIM:619077"
     exon            1..336
                     /gene="HSPA4L"
                     /gene_synonym="APG-1; APG1; HSPH3; Osp94"
                     /inference="alignment:Splign:2.1.0"
     CDS             230..2749
                     /gene="HSPA4L"
                     /gene_synonym="APG-1; APG1; HSPH3; Osp94"
                     /note="isoform 1 is encoded by transcript variant 1; heat
                     shock protein (hsp110 family); heat shock 70 kDa protein
                     4L; testis tissue sperm-binding protein Li 64n; heat shock
                     70 kDa protein 4-like protein; osmotic stress protein 94;
                     heat shock 70-related protein APG-1; heat shock 70kDa
                     protein 4-like; HSPA4-like protein; heat-shock protein
                     family A member 4-like protein; heat shock protein family
                     H member 3"
                     /codon_start=1
                     /product="heat shock 70 kDa protein 4L isoform 1"
                     /protein_id="NP_055093.2"
                     /db_xref="CCDS:CCDS3734.1"
                     /db_xref="GeneID:22824"
                     /db_xref="HGNC:HGNC:17041"
                     /db_xref="MIM:619077"
                     /translation="MSVVGIDLGFLNCYIAVARSGGIETIANEYSDRCTPACISLGSR
                     TRAIGNAAKSQIVTNVRNTIHGFKKLHGRSFDDPIVQTERIRLPYELQKMPNGSAGVK
                     VRYLEEERPFAIEQVTGMLLAKLKETSENALKKPVADCVISIPSFFTDAERRSVMAAA
                     QVAGLNCLRLMNETTAVALAYGIYKQDLPPLDEKPRNVVFIDMGHSAYQVLVCAFNKG
                     KLKVLATTFDPYLGGRNFDEALVDYFCDEFKTKYKINVKENSRALLRLYQECEKLKKL
                     MSANASDLPLNIECFMNDLDVSSKMNRAQFEQLCASLLARVEPPLKAVMEQANLQRED
                     ISSIEIVGGATRIPAVKEQITKFFLKDISTTLNADEAVARGCALQCAILSPAFKVREF
                     SITDLVPYSITLRWKTSFEDGSGECEVFCKNHPAPFSKVITFHKKEPFELEAFYTNLH
                     EVPYPDARIGSFTIQNVFPQSDGDSSKVKVKVRVNIHGIFSVASASVIEKQNLEGDHS
                     DAPMETETSFKNENKDNMDKMQVDQEEGHQKCHAEHTPEEEIDHTGAKTKSAVSDKQD
                     RLNQTLKKGKVKSIDLPIQSSLCRQLGQDLLNSYIENEGKMIMQDKLEKERNDAKNAV
                     EEYVYDFRDRLGTVYEKFITPEDLSKLSAVLEDTENWLYEDGEDQPKQVYVDKLQELK
                     KYGQPIQMKYMEHEERPKALNDLGKKIQLVMKVIEAYRNKDERYDHLDPTEMEKVEKC
                     ISDAMSWLNSKMNAQNKLSLTQDPVVKVSEIVAKSKELDNFCNPIIYKPKPKAEVPED
                     KPKANSEHNGPMDGQSGTETKSDSTKDSSQHTKSSGEMEVD"
     exon            337..394
                     /gene="HSPA4L"
                     /gene_synonym="APG-1; APG1; HSPH3; Osp94"
                     /inference="alignment:Splign:2.1.0"
     exon            395..535
                     /gene="HSPA4L"
                     /gene_synonym="APG-1; APG1; HSPH3; Osp94"
                     /inference="alignment:Splign:2.1.0"
     exon            536..658
                     /gene="HSPA4L"
                     /gene_synonym="APG-1; APG1; HSPH3; Osp94"
                     /inference="alignment:Splign:2.1.0"
     exon            659..758
                     /gene="HSPA4L"
                     /gene_synonym="APG-1; APG1; HSPH3; Osp94"
                     /inference="alignment:Splign:2.1.0"
     exon            759..892
                     /gene="HSPA4L"
                     /gene_synonym="APG-1; APG1; HSPH3; Osp94"
                     /inference="alignment:Splign:2.1.0"
     exon            893..1137
                     /gene="HSPA4L"
                     /gene_synonym="APG-1; APG1; HSPH3; Osp94"
                     /inference="alignment:Splign:2.1.0"
     exon            1138..1214
                     /gene="HSPA4L"
                     /gene_synonym="APG-1; APG1; HSPH3; Osp94"
                     /inference="alignment:Splign:2.1.0"
     exon            1215..1366
                     /gene="HSPA4L"
                     /gene_synonym="APG-1; APG1; HSPH3; Osp94"
                     /inference="alignment:Splign:2.1.0"
     exon            1367..1473
                     /gene="HSPA4L"
                     /gene_synonym="APG-1; APG1; HSPH3; Osp94"
                     /inference="alignment:Splign:2.1.0"
     exon            1474..1607
                     /gene="HSPA4L"
                     /gene_synonym="APG-1; APG1; HSPH3; Osp94"
                     /inference="alignment:Splign:2.1.0"
     exon            1608..1807
                     /gene="HSPA4L"
                     /gene_synonym="APG-1; APG1; HSPH3; Osp94"
                     /inference="alignment:Splign:2.1.0"
     exon            1808..1903
                     /gene="HSPA4L"
                     /gene_synonym="APG-1; APG1; HSPH3; Osp94"
                     /inference="alignment:Splign:2.1.0"
     exon            1904..2041
                     /gene="HSPA4L"
                     /gene_synonym="APG-1; APG1; HSPH3; Osp94"
                     /inference="alignment:Splign:2.1.0"
     exon            2042..2167
                     /gene="HSPA4L"
                     /gene_synonym="APG-1; APG1; HSPH3; Osp94"
                     /inference="alignment:Splign:2.1.0"
     exon            2168..2275
                     /gene="HSPA4L"
                     /gene_synonym="APG-1; APG1; HSPH3; Osp94"
                     /inference="alignment:Splign:2.1.0"
     exon            2276..2395
                     /gene="HSPA4L"
                     /gene_synonym="APG-1; APG1; HSPH3; Osp94"
                     /inference="alignment:Splign:2.1.0"
     exon            2396..2557
                     /gene="HSPA4L"
                     /gene_synonym="APG-1; APG1; HSPH3; Osp94"
                     /inference="alignment:Splign:2.1.0"
     exon            2558..10608
                     /gene="HSPA4L"
                     /gene_synonym="APG-1; APG1; HSPH3; Osp94"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3230..3235
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HSPA4L"
                     /gene_synonym="APG-1; APG1; HSPH3; Osp94"
                     /note="hexamer: AATAAG"
     polyA_site      3250
                     /gene="HSPA4L"
                     /gene_synonym="APG-1; APG1; HSPH3; Osp94"
                     /note="major polyA site"
     regulatory      10575..10580
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HSPA4L"
                     /gene_synonym="APG-1; APG1; HSPH3; Osp94"
                     /note="hexamer: AATAAA"
     polyA_site      10608
                     /gene="HSPA4L"
                     /gene_synonym="APG-1; APG1; HSPH3; Osp94"
                     /note="major polyA site"
ORIGIN      
        1 agtagggaaa gacccaggct gcgggacgcg gtgcaggctg cggcgctgac ggcctctgct
       61 ccttccgcgg gtttccgact ccctgcccta gattttctgc ttagcgactt ggggtcccct
      121 ctcgtttgct tctggtagga gtcgcaatcc cagcagcaat agcccagaag aggacacggt
      181 tcccgtaccg aagggttcag taccagcagc ccgaccatca cgcggcggga tgtctgtggt
      241 tggcattgac ctcggctttc tcaactgcta cattgctgtc gcgagaagtg gcggcatcga
      301 gaccatcgcc aatgagtaca gcgacaggtg taccccggcc tgtatatcat tgggatcaag
      361 aactcgagcc attggaaatg cagcaaagag ccagatagtc acgaacgtaa gaaatacaat
      421 tcatggcttc aaaaagcttc atgggcgatc atttgatgat cccattgtgc aaactgaaag
      481 gatcaggctt ccctatgaac tgcagaaaat gcctaatgga agtgcaggag ttaaggtgcg
      541 gtacttagag gaagagagac cttttgcaat tgagcaagtt actggaatgc tgttagccaa
      601 gcttaaagag acttcagaaa atgctttgaa gaaaccagtg gctgactgtg tgatttcaat
      661 tcctagcttt tttactgatg ccgagagaag atctgtgatg gctgcagccc aggttgcagg
      721 cttaaattgt ttaaggttga tgaatgaaac tactgcagtt gcactggcgt atggaattta
      781 taaacaggat cttcccccat tagatgagaa accaagaaat gtagtattta ttgatatggg
      841 acattctgcc tatcaggtct tggtttgtgc ttttaacaaa ggaaaactta aagtcttggc
      901 tactaccttt gatccatatt tgggtggcag gaactttgat gaggctttag tagactactt
      961 ctgtgatgag ttcaagacca aatataagat aaatgtgaaa gaaaactctc gggccttgtt
     1021 gcgtttatat caggaatgtg aaaaactaaa gaagctaatg agtgcaaatg catcagatct
     1081 tccattgaac attgagtgtt tcatgaatga ccttgatgtt tctagtaaaa tgaacagggc
     1141 tcaatttgaa caactgtgtg cttccctttt ggccagggtt gaaccacctt taaaagcagt
     1201 aatggaacaa gctaacttac aacgtgaaga cattagtagt atagaaattg taggaggagc
     1261 aacacgaatt cctgcagtga aagaacaaat cactaaattc tttcttaaag acataagtac
     1321 cacattaaat gctgatgaag ctgttgcaag aggatgtgcg ttacagtgtg cgattctctc
     1381 accagcattt aaagtgcgtg aattttccat aacagacctt gttccctatt caatcacatt
     1441 aaggtggaag acctcttttg aagatggaag tggggaatgt gaagttttct gtaagaacca
     1501 tcctgcccca ttctcaaaag tcattacttt ccacaagaag gaaccatttg aactagaagc
     1561 attttatact aatttacatg aagtgcctta tcctgatgca agaattggga gcttcactat
     1621 tcagaatgtt tttccacagt ctgatggtga tagttccaaa gtgaaggtta aagttcgtgt
     1681 taacatccat ggaatcttca gtgtggctag cgcatcagta attgagaagc aaaatttgga
     1741 aggcgatcac agtgatgctc caatggagac agaaacttca tttaaaaatg aaaacaaaga
     1801 taatatggat aaaatgcagg ttgatcaaga agaagggcat caaaaatgtc atgctgaaca
     1861 cactccagaa gaggaaattg atcatacagg agccaaaaca aagtcagctg tctcagacaa
     1921 acaagaccga ttaaatcaga cacttaaaaa aggaaaagtc aaaagtattg atctaccgat
     1981 ccagagtagc ctatgtagac aactaggcca agatcttctc aacagctaca ttgaaaatga
     2041 ggggaagatg atcatgcaag ataagttaga gaaagaaaga aatgatgcta agaatgccgt
     2101 tgaagaatat gtatatgatt ttagagacag gctgggcact gtctatgaaa aattcatcac
     2161 tccagaagac ttgagtaaac tgtctgcagt attggaagac acagaaaatt ggctttatga
     2221 agacggagag gaccaaccta aacaagttta tgtggataag cttcaagaac taaagaaata
     2281 cggccagcct attcaaatga agtacatgga gcatgaagag agaccaaaag ccttaaatga
     2341 cttgggaaaa aagatccaac ttgtcatgaa agtgatagaa gcttatagaa acaaggatga
     2401 aagatatgat catctggatc ctactgaaat ggaaaaggtt gaaaaatgta tcagtgatgc
     2461 catgagttgg ctgaatagta agatgaatgc acagaacaaa ctaagtctca ctcaagatcc
     2521 tgtggtaaaa gtttcagaaa tagtagcaaa gtcaaaggaa ctggataatt tctgtaaccc
     2581 catcatttac aagcccaaac caaaagcaga agttcctgaa gacaaaccaa aagctaatag
     2641 tgaacacaat ggcccaatgg atggacagag tggaactgaa actaaatcag attcaacaaa
     2701 agacagctca cagcatacta aatcctctgg agagatggaa gtggactaag tcttaatttt
     2761 accttcacat taattcaaac cgtgcaagta accacggggt ccatctttta catctggtac
     2821 acacaacaga cgctcagttg ttcttaacca cttttgtcat ttgttttttg gagtagtttt
     2881 gaaaagtgtt ttatattgag tgcacttctg ttcatttcca ttgctgctta tatgcagtgt
     2941 tagccgaatt agatttacaa gacaatctaa gctttccgga taattttata tatcaaacat
     3001 acaggatgga tacatagttg gcaacagtct accttattta aagcttctac tgggataaac
     3061 ctcaattcct ttattcagga aaggatactt tattgcatta ttgttgcaga agcatagatt
     3121 taattgcatc tttattttga aaaacaaatg aaaattgatg gggtttaaag ctacagaggc
     3181 actgaccttt ttctagttat tgtattgcta caatttaaat attaaaacaa ataagagctt
     3241 tctcaacaaa ttagtccatc tcattgatgt accacagaaa tccaaagctt tgctgtcatc
     3301 acataaatga attaaatcac atgtgaaagg gaacacaccc aattttatgt taaggtgaaa
     3361 ccagcagttt gcttctttta ttagtaatgt ggattgatgt tttctcagag taactcgttg
     3421 tcttttcgct tgaaattttt gatcttgtcc tgaagactag ctgctgctaa aacaatgcta
     3481 gcctaaagat acaggagtct ctgacaaaat gactttcatg ttaaggggaa atgctgttta
     3541 tctactcaga catgcatctg tgatgttcat agccttgttt cagttataat agttgtcttg
     3601 tttttttctt aattgatttt gttaataata ccttaacaca gggtaggaag aagtggcaca
     3661 gtgtattgca ttatatacat ttatcattga tttacctaat gttaccttgt agattaaaca
     3721 ctattaagtg gtaatacttg aaaaggagca cttataccat aagtcttagg aaataatgtt
     3781 tgtaataaac ttaactatgt tacatatcaa caggcaaaaa tatagaatgt tacatagtgt
     3841 tgtgtgatta aattatagtt cctgctgtta acattacctt ttgaaacctt ggctccagtt
     3901 ttggtgcttc ttatacaaat taaatgcgaa aagggactgt aaacttaaga tgtattttaa
     3961 ggactttatc tgtgcttcat cacccaactt gttcacattg ttaatttctg tccagagacc
     4021 tgaaactgct taagtatggg aacagactga aatggtgttt caacctgcaa gagacagaag
     4081 catagacaag tatatgagcc cttatgtcct taatgccaat caactagatt gacctaggtt
     4141 aaaatttcaa aaggctcatt ttctcccaaa gctaaatctt tatattagga acaattaaat
     4201 attgacttaa gttgtaaaaa ttgagagtac aatatcttta gggatagtca ttatttctca
     4261 caactagttc tctacgagag attttgtttc tacaatgtag tcagcactta atggagagta
     4321 tggagtagtg cattttgcag ggatttttat tccgtctttt gaggggaaga atctcttttt
     4381 tgcattaaaa aatcatataa acttaatata tttttaaact ctcctagtct ttaaactttc
     4441 tcttaattct cttaaaatga aataggtata tccaataggt gatgaaatac attttttaca
     4501 aacatagatg ctttttattt gtatgttgaa tgtacagatt catttcaaga aaagcatgaa
     4561 gataatgaaa gcaaatatgt aacaatacaa gtagggactt tagagcttct atttgggtta
     4621 aaacatccac tcacttggtt aggagctatg cctcagaaac ttcctgggag taaatatggg
     4681 aacaactttt ctattaatac atctaaattt tctagtatat cctgacattt tccattcctg
     4741 ctctgaagtc acttttagtt ttgtgtttta gtcagatagt accactaatt tttgtaatta
     4801 tataacactt catttaataa cttatataca tccaaatata tatacttttt ctacccaatt
     4861 attcaactta aaagatttca aaaacgattt agccttataa tgtatttatt tatttaattc
     4921 attaatgaaa gagagtgctc tctttaatgc agctttgggt agacagtcta attcagtcca
     4981 attttaatct gcattaaaat atgcaatgct agacaaagat tttcaaaata aaaagctatt
     5041 atttcagaaa ttattttaaa agatgcatag taaaacagtg ttttcctatg gtaagcaaat
     5101 aagaatgaag tttgtaggga atatttggaa tgattaatat aaattagtat actttggctc
     5161 tgtggaaaag tgtaaattgt ggttttagaa accaactctt gatagttgta ctgtgattat
     5221 aaaaaacaat tgttaaataa tataatggaa gtttgtgata attccttagc agtaatgtaa
     5281 aattattaag ttttggccag gcgctgtggc tcacgcctgt aatcctagca ctttgggagg
     5341 ccaaggcagg cagatcacct gaggtcagga gttcaagacc agcctggcca acatggtgaa
     5401 accctgtctc tactaaaaat acaaaaaagt agccgggcgt ggtggcatgt ccctgtaatc
     5461 tcagctaccc gggaggctca ggcaggagaa tcgctggaac ccaggaggca gaggctgccg
     5521 ttagctgaga tcgtgctcct gcactccagc ctgggcaaca gaacaagact ccgtctcaaa
     5581 aaaaaaacaa aaattcttcc aaaaaactag aaagagcaca gcaaagaatt tggctattga
     5641 aataacagat aaacttgatt ttgaaaagga taacaaggct gggcacagtg actcacacct
     5701 gtaatcctag cactttgaga ggctaagaag ggaggatcac ttgaacccag gagttcaaga
     5761 ccagcctgag aaacatagtg agaccctgtc tctaaaaaaa taaaaactta gcaaggcatg
     5821 gtggtgcaca cctgtagtcc tagctactca ggaggctgag gtggaggatc acttaagcct
     5881 gagaggtcaa ggttgcaatg agccataatc ataccactgc actccagcct gggcaacaga
     5941 gtgaggccct gtctcaaaca cataaaaggc agtggcccac gcctgtaatc ccagcacttt
     6001 gggaggccaa ggcgggcaga tcacgaggtc aggagatcaa gaccatcctg gctaacacgg
     6061 tgaaaccccg tctctactaa aaatacaaaa aattagctgg gcatggtggc gggcgccggt
     6121 agtcccagct actcaggagg ctgaggcagg agaatggtgt gaacccagga ggcggaggtt
     6181 gcagtgagcc gagatcgcgc cactgcactc cagcctgggc aacagagcaa gactccatct
     6241 caaaaacaaa acaaaacaaa aacataaaag gaaaacaaga catttactaa gaagagctaa
     6301 aggaaagaat taaaggtttc ttatgctttt ttgggggggg gtgtcaattt ttagtatatt
     6361 aagagctaaa agaagtaaag gtttcttaga ctttttttgg gagggaggcg tcaattttta
     6421 gtatatttgt gattaattcc atgatagaag tctttaaaga gaattttggg gctcaataat
     6481 ttaacataat gtcagtgtta aagcacttaa tccaaattag cccatcttta actgttttga
     6541 aaaatctata tttattaaag gtagtagaca tttgaatata gtaatcaaat taactgattt
     6601 tgttttcacc ctaaatacat acaaccattc ctccactttc ataaatttga caaataataa
     6661 gcctctccca ttaggatttt ctgttttata gtaaatagtt ggtatttggt agtccacaat
     6721 taaagtcatt gcattaattt tacaaattat agatttagaa atgattctta agttattggt
     6781 aaaatataac tttctcagtt tttaaaagtt tacaaaattt ttttttttgt tttgagacag
     6841 agtatggctt tgttacccag gctggattgc aatggggtga tctaggctca ctgcagcctc
     6901 caccacctga gttcaagcga tttttctgcc tcaacctctc ctgagtagct gggactacag
     6961 gtgcatgaca ccacacccag ctaatttttg tatttttata gagatggagt tttgccatgt
     7021 tggccagcct ggtcttgaac tcttgaactc ctgacctcag gtaatccacc cacctcagac
     7081 tcccaaagtg ctgggattac aggcgtgagc caccataccc ggccaaaaat ttttgctttt
     7141 aaagttaatg ttattttgtg gttaaacttc taaagttttg aactgtaagc agaattttgt
     7201 tttgtgatat tttattgtat aaactgttaa tgagaggcac agctaattgt acatatcaat
     7261 atacatttaa aatctggtaa cactggttaa atagcccttg atgacttttc atgtggcatg
     7321 agagggatat gcttataaag cttaattctg atattatcct cttactacct acagtatgtt
     7381 ttgcaaaaat cagtccactt agcaaactaa tctttgtaaa gcagtcagtt tcagaagata
     7441 ctttttatca aaaaagatgg caggtttaac attatacctt ttggtttttg cccaacattt
     7501 gatttaatct aaagcaagaa tataaaataa ttttaagaag catataattt cttttgataa
     7561 aaagtaacaa aaatttaatg cagatcaaag accaaggctt gtaaccaaaa caagcaaaaa
     7621 gaaactttag ctgtttaact atcacctctc taatttaaaa tgcatgaaaa ttaatacttt
     7681 gtttttgttt tttttttgga aacagtctca ctctgtcacc caggctggag ggcacaatct
     7741 ctgctcactg caacctccac ctcctgggct caagcctcaa gcaattctcc tgcctcagcc
     7801 tcccaagtag ctgggattac aggaacccac caccacgccc agctaatttt tgtattttta
     7861 gtagatgggg tttcatcatg ttggccaggc tggttttgaa ctcctgactt caaatgatcc
     7921 acccacatcc acctgccaga gtgctgagat tacaggcgtg agccaccgca cctggccaat
     7981 gttcttttat atactcatgt tttctaacaa aacagctttg gatttataat gctgccagat
     8041 gtcattacaa ttttttttct aaattcaaag gtaattattc taaacaataa aagggcacct
     8101 ggaattttca gaacattaaa aaactgaaac cacttagaag aaaagaagaa acctaaatgt
     8161 ctgtggaagt gatcagtatt aagtgggtga tggctctttc atcttcctta tccctaaaga
     8221 tacttccaac atattgttag agtaagtatt aaaagaaaaa gtaggtttgc tgaaaaacat
     8281 tactaaatga cttggagctc tccagtcaaa gaaacaaagc taattgaaat aatgtacatt
     8341 tggttcagtc tgtcaggcaa gtacaaatcc ataacaaaaa aaattaatag tatgctcttt
     8401 gctttggaat ctctaattta cggtcatgcc ctaagtcaca ggatgattat tagggaaatg
     8461 tcattgacac ctgaaaatta cctatcctgt aaaaagactg gtgaaattca acatgtttta
     8521 aataatttaa ttgcattata gcatataaac tataaatgaa aatgatcttt gtctttcctt
     8581 tatcttgctt tatcctctta tccaattcta gactcttaaa taatcactgt atttttctca
     8641 agtatttgtt attttttatt atcctttaga tctttttgtt catggctctt taaaaatagt
     8701 tttactgata atccaatcac ttggatagtt aattttatta ctttttgttt cctttttaat
     8761 atttattctt cccgttaagg gttttttggc tataaattcg tacttgataa gctatgttat
     8821 ttctttgtgt aatttaatag tagcattctg gagtagattc tttttttgta ttctggcctt
     8881 ggaatacttt aattccggac caaaagaggt caattttgtg agtattgcct attaagtaaa
     8941 caacatccct catttttaat ccaaatatca atttgacaga ataacaaggg tcagaaattc
     9001 aaaatagctg ttagacacct ttgattcttt gctgctctct ggttatgtat gtgtgttgtg
     9061 catcttcctt gttgttttag agtttcctaa attttttgct tctggggact tgagtatttt
     9121 taaatgttct taaaattata tgaatctacc cagaatgtag atgtcaaaat gtaattagag
     9181 gccccgtgca gtggctcacg cctgtaatcc cagcactttg ggaggccgag gcaggtggat
     9241 cgcctgagct caggagttca agaccagcct gggcaacatg gcaaaacccc gtctctacta
     9301 aaaatacaaa aaattagctg ggtgtggtgg tgtgtgcctg tagtcccagc tactcgggag
     9361 gctgaggcag gagaatcgct tgaaccctgg cgaaacccca tttctaaaat agaaaaattt
     9421 agccgggtgt ggtggtgtgt gcctatagtc ccagctattc gggaggctga ggcaagagaa
     9481 ttgcttgaac ccgggaggtg gaggtcgcag tgagctgaga tcctgccact gcactccaac
     9541 ctgggggtaa cagagcgaga ctctgtctca aaaaaaaaaa aaagtgtaat tagaaatgga
     9601 aatctaggta aaggaagctt taaaatgttg tattttttcc ctgaaataaa cttttatgat
     9661 ttaatgtctt ggaaaaagac tcacatgaaa agtggctttt atatttggta ggataaagcc
     9721 attgtccttt aataccatag caaatagaag caatttttaa tgaggttact gaattgttta
     9781 gtattcctaa tatggcatcc aggtcatctt ttcttttctc caaccattat taaaataact
     9841 tgacactttg ggaggccaag ctgggcagat cacctgaggt caggagtttg agaccagcct
     9901 ggccaacatg gtgaaacccc atctctacta aaaaaaatac aaaaattagc ggggccatgg
     9961 tgacgtgcac ctgtaatccc agctactcgg gaggctgagg tgggagaata gcttgaacct
    10021 gggaggcaga ggttgtggtg agccaagatc acgccactgc actccagcct gggtgacaga
    10081 gcaagactcc atctcaagaa aaaaataaat aaataataat ttgtgtatgt gatgactgac
    10141 tctagtcatt atggaaaata acttttggca gtttagttcc taatgttaac aattcctctt
    10201 tttaagagag gtactacatt tgatttctca atttctcagt ttgttttcaa tacaaacagc
    10261 aaccactgaa atgcagaaaa tggtaatcaa gtgtgatgtt tctataaaaa taattgagat
    10321 tcacatgtaa tagacccttt agctttaatg agcagacatg agaatggttt atgatcttaa
    10381 gttggtaaca taggaatatg tatttgtcaa ttttagttca caaacatatc cataagaatg
    10441 tttacacatg gcagtatcaa agcatataaa atattttcta tgtaagtaaa ttgcatcttt
    10501 atgctagtga acaaagtagc aataaatgtt catttttgga tggcagtaca cattgttaac
    10561 aatcgtgtaa ttataataaa tatgtctacc tactccttga accatgaa
//
