LOCUS       NM_003299               2782 bp    mRNA    linear   PRI 20-FEB-2025
DEFINITION  Homo sapiens heat shock protein 90 beta family member 1 (HSP90B1),
            mRNA.
ACCESSION   NM_003299
VERSION     NM_003299.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Ma,M., Dubey,R., Jen,A., Pusapati,G.V., Singal,B., Shishkova,E.,
            Overmyer,K.A., Cormier-Daire,V., Fedry,J., Aravind,L., Coon,J.J.
            and Rohatgi,R.
  TITLE     Regulated N-glycosylation controls chaperone function and receptor
            trafficking
  JOURNAL   Science 386 (6722), 667-672 (2024)
   PUBMED   39509507
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Huang,X., Zhang,W., Yang,N., Zhang,Y., Qin,T., Ruan,H., Zhang,Y.,
            Tian,C., Mo,X., Tang,W., Liu,J. and Zhang,B.
  TITLE     Identification of HSP90B1 in pan-cancer hallmarks to aid
            development of a potential therapeutic target
  JOURNAL   Mol Cancer 23 (1), 19 (2024)
   PUBMED   38243263
  REMARK    GeneRIF: Identification of HSP90B1 in pan-cancer hallmarks to aid
            development of a potential therapeutic target.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2782)
  AUTHORS   Zuehlke,A. and Johnson,J.L.
  TITLE     Hsp90 and co-chaperones twist the functions of diverse client
            proteins
  JOURNAL   Biopolymers 93 (3), 211-217 (2010)
   PUBMED   19697319
  REMARK    Review article
REFERENCE   4  (bases 1 to 2782)
  AUTHORS   Chen,B., Piel,W.H., Gui,L., Bruford,E. and Monteiro,A.
  TITLE     The HSP90 family of genes in the human genome: insights into their
            divergence and evolution
  JOURNAL   Genomics 86 (6), 627-637 (2005)
   PUBMED   16269234
REFERENCE   5  (bases 1 to 2782)
  AUTHORS   Prasadarao,N.V., Srivastava,P.K., Rudrabhatla,R.S., Kim,K.S.,
            Huang,S.H. and Sukumaran,S.K.
  TITLE     Cloning and expression of the Escherichia coli K1 outer membrane
            protein A receptor, a gp96 homologue
  JOURNAL   Infect Immun 71 (4), 1680-1688 (2003)
   PUBMED   12654781
  REMARK    GeneRIF: OmpA of E. coli K1 interacts with a gp96-like molecule on
            HBMECs for invasion (Ecgp: 96-kDa human brain microvascular
            endothelial cell (HBMEC) glycoprotein)
REFERENCE   6  (bases 1 to 2782)
  AUTHORS   Maki,R.G., Eddy,R.L. Jr., Byers,M., Shows,T.B. and Srivastava,P.K.
  TITLE     Mapping of the genes for human endoplasmic reticular heat shock
            protein gp96/grp94
  JOURNAL   Somat Cell Mol Genet 19 (1), 73-81 (1993)
   PUBMED   8460400
REFERENCE   7  (bases 1 to 2782)
  AUTHORS   Schaiff,W.T., Hruska,K.A. Jr., McCourt,D.W., Green,M. and
            Schwartz,B.D.
  TITLE     HLA-DR associates with specific stress proteins and is retained in
            the endoplasmic reticulum in invariant chain negative cells
  JOURNAL   J Exp Med 176 (3), 657-666 (1992)
   PUBMED   1512535
REFERENCE   8  (bases 1 to 2782)
  AUTHORS   Liu,E.S. and Lee,A.S.
  TITLE     Common sets of nuclear factors binding to the conserved promoter
            sequence motif of two coordinately regulated ER protein genes,
            GRP78 and GRP94
  JOURNAL   Nucleic Acids Res 19 (19), 5425-5431 (1991)
   PUBMED   1923827
REFERENCE   9  (bases 1 to 2782)
  AUTHORS   Maki,R.G., Old,L.J. and Srivastava,P.K.
  TITLE     Human homologue of murine tumor rejection antigen gp96:
            5'-regulatory and coding regions and relationship to stress-induced
            proteins
  JOURNAL   Proc Natl Acad Sci U S A 87 (15), 5658-5662 (1990)
   PUBMED   2377606
  REMARK    GeneRIF: a paper describing a pathway in which this gene product
            functions
REFERENCE   10 (bases 1 to 2782)
  AUTHORS   Srivastava,P.K. and Old,L.J.
  TITLE     Identification of a human homologue of the murine tumor rejection
            antigen GP96
  JOURNAL   Cancer Res 49 (6), 1341-1343 (1989)
   PUBMED   2924290
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC078819.24, X15187.1 and
            CA419931.1.
            
            On Nov 23, 2018 this sequence version replaced NM_003299.2.
            
            Summary: This gene encodes a member of a family of adenosine
            triphosphate(ATP)-metabolizing molecular chaperones with roles in
            stabilizing and folding other proteins. The encoded protein is
            localized to melanosomes and the endoplasmic reticulum. Expression
            of this protein is associated with a variety of pathogenic states,
            including tumor formation. There is a microRNA gene located within
            the 5' exon of this gene. There are pseudogenes for this gene on
            chromosomes 1 and 15. [provided by RefSeq, Aug 2012].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: GQ900941.1, AJ890084.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000299767.10/ ENSP00000299767.4
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1                 AC078819.24        6982-6982
            2-2781              X15187.1           1-2780
            2782-2782           CA419931.1         22-22               c
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q23.3"
     gene            1..2782
                     /gene="HSP90B1"
                     /gene_synonym="ECGP; GP96; GRP94; HEL-S-125m; HEL35; TRA1"
                     /note="heat shock protein 90 beta family member 1"
                     /db_xref="GeneID:7184"
                     /db_xref="HGNC:HGNC:12028"
                     /db_xref="MIM:191175"
     exon            1..155
                     /gene="HSP90B1"
                     /gene_synonym="ECGP; GP96; GRP94; HEL-S-125m; HEL35; TRA1"
                     /inference="alignment:Splign:2.1.0"
     CDS             107..2518
                     /gene="HSP90B1"
                     /gene_synonym="ECGP; GP96; GRP94; HEL-S-125m; HEL35; TRA1"
                     /note="tumor rejection antigen (gp96) 1; endothelial cell
                     (HBMEC) glycoprotein; endoplasmin; 94 kDa
                     glucose-regulated protein; heat shock protein 90 kDa beta
                     member 1; tumor rejection antigen 1; stress-inducible
                     tumor rejection antigen gp96; heat shock protein 90kDa
                     beta family member 1; epididymis luminal protein 35;
                     epididymis secretory sperm binding protein Li 125m; heat
                     shock protein 90kDa beta (Grp94), member 1; heat shock
                     protein family C member 4"
                     /codon_start=1
                     /product="endoplasmin precursor"
                     /protein_id="NP_003290.1"
                     /db_xref="CCDS:CCDS9094.1"
                     /db_xref="GeneID:7184"
                     /db_xref="HGNC:HGNC:12028"
                     /db_xref="MIM:191175"
                     /translation="MRALWVLGLCCVLLTFGSVRADDEVDVDGTVEEDLGKSREGSRT
                     DDEVVQREEEAIQLDGLNASQIRELREKSEKFAFQAEVNRMMKLIINSLYKNKEIFLR
                     ELISNASDALDKIRLISLTDENALSGNEELTVKIKCDKEKNLLHVTDTGVGMTREELV
                     KNLGTIAKSGTSEFLNKMTEAQEDGQSTSELIGQFGVGFYSAFLVADKVIVTSKHNND
                     TQHIWESDSNEFSVIADPRGNTLGRGTTITLVLKEEASDYLELDTIKNLVKKYSQFIN
                     FPIYVWSSKTETVEEPMEEEEAAKEEKEESDDEAAVEEEEEEKKPKTKKVEKTVWDWE
                     LMNDIKPIWQRPSKEVEEDEYKAFYKSFSKESDDPMAYIHFTAEGEVTFKSILFVPTS
                     APRGLFDEYGSKKSDYIKLYVRRVFITDDFHDMMPKYLNFVKGVVDSDDLPLNVSRET
                     LQQHKLLKVIRKKLVRKTLDMIKKIADDKYNDTFWKEFGTNIKLGVIEDHSNRTRLAK
                     LLRFQSSHHPTDITSLDQYVERMKEKQDKIYFMAGSSRKEAESSPFVERLLKKGYEVI
                     YLTEPVDEYCIQALPEFDGKRFQNVAKEGVKFDESEKTKESREAVEKEFEPLLNWMKD
                     KALKDKIEKAVVSQRLTESPCALVASQYGWSGNMERIMKAQAYQTGKDISTNYYASQK
                     KTFEINPRHPLIRDMLRRIKEDEDDKTVLDLAVVLFETATLRSGYLLPDTKAYGDRIE
                     RMLRLSLNIDPDAKVEEEPEEEPEETAEDTTEDTEQDEDEEMDVGTDEEEETAKESTA
                     EKDEL"
     exon            156..258
                     /gene="HSP90B1"
                     /gene_synonym="ECGP; GP96; GRP94; HEL-S-125m; HEL35; TRA1"
                     /inference="alignment:Splign:2.1.0"
     exon            259..400
                     /gene="HSP90B1"
                     /gene_synonym="ECGP; GP96; GRP94; HEL-S-125m; HEL35; TRA1"
                     /inference="alignment:Splign:2.1.0"
     exon            401..517
                     /gene="HSP90B1"
                     /gene_synonym="ECGP; GP96; GRP94; HEL-S-125m; HEL35; TRA1"
                     /inference="alignment:Splign:2.1.0"
     exon            518..849
                     /gene="HSP90B1"
                     /gene_synonym="ECGP; GP96; GRP94; HEL-S-125m; HEL35; TRA1"
                     /inference="alignment:Splign:2.1.0"
     exon            850..961
                     /gene="HSP90B1"
                     /gene_synonym="ECGP; GP96; GRP94; HEL-S-125m; HEL35; TRA1"
                     /inference="alignment:Splign:2.1.0"
     exon            962..1081
                     /gene="HSP90B1"
                     /gene_synonym="ECGP; GP96; GRP94; HEL-S-125m; HEL35; TRA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1082..1198
                     /gene="HSP90B1"
                     /gene_synonym="ECGP; GP96; GRP94; HEL-S-125m; HEL35; TRA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1199..1336
                     /gene="HSP90B1"
                     /gene_synonym="ECGP; GP96; GRP94; HEL-S-125m; HEL35; TRA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1337..1414
                     /gene="HSP90B1"
                     /gene_synonym="ECGP; GP96; GRP94; HEL-S-125m; HEL35; TRA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1415..1480
                     /gene="HSP90B1"
                     /gene_synonym="ECGP; GP96; GRP94; HEL-S-125m; HEL35; TRA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1481..1750
                     /gene="HSP90B1"
                     /gene_synonym="ECGP; GP96; GRP94; HEL-S-125m; HEL35; TRA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1751..1996
                     /gene="HSP90B1"
                     /gene_synonym="ECGP; GP96; GRP94; HEL-S-125m; HEL35; TRA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1997..2133
                     /gene="HSP90B1"
                     /gene_synonym="ECGP; GP96; GRP94; HEL-S-125m; HEL35; TRA1"
                     /inference="alignment:Splign:2.1.0"
     exon            2134..2212
                     /gene="HSP90B1"
                     /gene_synonym="ECGP; GP96; GRP94; HEL-S-125m; HEL35; TRA1"
                     /inference="alignment:Splign:2.1.0"
     exon            2213..2368
                     /gene="HSP90B1"
                     /gene_synonym="ECGP; GP96; GRP94; HEL-S-125m; HEL35; TRA1"
                     /inference="alignment:Splign:2.1.0"
     exon            2369..2488
                     /gene="HSP90B1"
                     /gene_synonym="ECGP; GP96; GRP94; HEL-S-125m; HEL35; TRA1"
                     /inference="alignment:Splign:2.1.0"
     exon            2489..2782
                     /gene="HSP90B1"
                     /gene_synonym="ECGP; GP96; GRP94; HEL-S-125m; HEL35; TRA1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2763..2768
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HSP90B1"
                     /gene_synonym="ECGP; GP96; GRP94; HEL-S-125m; HEL35; TRA1"
                     /note="hexamer: AATAAA"
     polyA_site      2782
                     /gene="HSP90B1"
                     /gene_synonym="ECGP; GP96; GRP94; HEL-S-125m; HEL35; TRA1"
                     /note="major polyA site"
ORIGIN      
        1 agtgggcgga ccgcgcggct ggaggtgtga ggatccgaac ccaggggtgg ggggtggagg
       61 cggctcctgc gatcgaaggg gacttgagac tcaccggccg cacgccatga gggccctgtg
      121 ggtgctgggc ctctgctgcg tcctgctgac cttcgggtcg gtcagagctg acgatgaagt
      181 tgatgtggat ggtacagtag aagaggatct gggtaaaagt agagaaggat caaggacgga
      241 tgatgaagta gtacagagag aggaagaagc tattcagttg gatggattaa atgcatcaca
      301 aataagagaa cttagagaga agtcggaaaa gtttgccttc caagccgaag ttaacagaat
      361 gatgaaactt atcatcaatt cattgtataa aaataaagag attttcctga gagaactgat
      421 ttcaaatgct tctgatgctt tagataagat aaggctaata tcactgactg atgaaaatgc
      481 tctttctgga aatgaggaac taacagtcaa aattaagtgt gataaggaga agaacctgct
      541 gcatgtcaca gacaccggtg taggaatgac cagagaagag ttggttaaaa accttggtac
      601 catagccaaa tctgggacaa gcgagttttt aaacaaaatg actgaagcac aggaagatgg
      661 ccagtcaact tctgaattga ttggccagtt tggtgtcggt ttctattccg ccttccttgt
      721 agcagataag gttattgtca cttcaaaaca caacaacgat acccagcaca tctgggagtc
      781 tgactccaat gaattttctg taattgctga cccaagagga aacactctag gacggggaac
      841 gacaattacc cttgtcttaa aagaagaagc atctgattac cttgaattgg atacaattaa
      901 aaatctcgtc aaaaaatatt cacagttcat aaactttcct atttatgtat ggagcagcaa
      961 gactgaaact gttgaggagc ccatggagga agaagaagca gccaaagaag agaaagaaga
     1021 atctgatgat gaagctgcag tagaggaaga agaagaagaa aagaaaccaa agactaaaaa
     1081 agttgaaaaa actgtctggg actgggaact tatgaatgat atcaaaccaa tatggcagag
     1141 accatcaaaa gaagtagaag aagatgaata caaagctttc tacaaatcat tttcaaagga
     1201 aagtgatgac cccatggctt atattcactt tactgctgaa ggggaagtta ccttcaaatc
     1261 aattttattt gtacccacat ctgctccacg tggtctgttt gacgaatatg gatctaaaaa
     1321 gagcgattac attaagctct atgtgcgccg tgtattcatc acagacgact tccatgatat
     1381 gatgcctaaa tacctcaatt ttgtcaaggg tgtggtggac tcagatgatc tccccttgaa
     1441 tgtttcccgc gagactcttc agcaacataa actgcttaag gtgattagga agaagcttgt
     1501 tcgtaaaacg ctggacatga tcaagaagat tgctgatgat aaatacaatg atactttttg
     1561 gaaagaattt ggtaccaaca tcaagcttgg tgtgattgaa gaccactcga atcgaacacg
     1621 tcttgctaaa cttcttaggt tccagtcttc tcatcatcca actgacatta ctagcctaga
     1681 ccagtatgtg gaaagaatga aggaaaaaca agacaaaatc tacttcatgg ctgggtccag
     1741 cagaaaagag gctgaatctt ctccatttgt tgagcgactt ctgaaaaagg gctatgaagt
     1801 tatttacctc acagaacctg tggatgaata ctgtattcag gcccttcccg aatttgatgg
     1861 gaagaggttc cagaatgttg ccaaggaagg agtgaagttc gatgaaagtg agaaaactaa
     1921 ggagagtcgt gaagcagttg agaaagaatt tgagcctctg ctgaattgga tgaaagataa
     1981 agcccttaag gacaagattg aaaaggctgt ggtgtctcag cgcctgacag aatctccgtg
     2041 tgctttggtg gccagccagt acggatggtc tggcaacatg gagagaatca tgaaagcaca
     2101 agcgtaccaa acgggcaagg acatctctac aaattactat gcgagtcaga agaaaacatt
     2161 tgaaattaat cccagacacc cgctgatcag agacatgctt cgacgaatta aggaagatga
     2221 agatgataaa acagttttgg atcttgctgt ggttttgttt gaaacagcaa cgcttcggtc
     2281 agggtatctt ttaccagaca ctaaagcata tggagataga atagaaagaa tgcttcgcct
     2341 cagtttgaac attgaccctg atgcaaaggt ggaagaagag cccgaagaag aacctgaaga
     2401 gacagcagaa gacacaacag aagacacaga gcaagacgaa gatgaagaaa tggatgtggg
     2461 aacagatgaa gaagaagaaa cagcaaagga atctacagct gaaaaagatg aattgtaaat
     2521 tatactctca ccatttggat cctgtgtgga gagggaatgt gaaatttaca tcatttcttt
     2581 ttgggagaga cttgttttgg atgcccccta atccccttct cccctgcact gtaaaatgtg
     2641 ggattatggg tcacaggaaa aagtgggttt tttagttgaa ttttttttaa cattcctcat
     2701 gaatgtaaat ttgtactatt taactgacta ttcttgatgt aaaatcttgt catgtgtata
     2761 aaaataaaaa agatcccaaa ta
//
