LOCUS       NM_001537               8649 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens heat shock factor binding protein 1 (HSBP1), mRNA.
ACCESSION   NM_001537
VERSION     NM_001537.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8649)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 8649)
  AUTHORS   Zhong,Y., Cao,L., Ma,H., Wang,Q., Wei,P., Yang,J., Mo,Y., Cao,L.,
            Shuai,C. and Peng,S.
  TITLE     Lin28A Regulates Stem-like Properties of Ovarian Cancer Cells by
            Enriching RAN and HSBP1 mRNA and Up-regulating its Protein
            Expression
  JOURNAL   Int J Biol Sci 16 (11), 1941-1953 (2020)
   PUBMED   32398961
  REMARK    GeneRIF: Lin28A Regulates Stem-like Properties of Ovarian Cancer
            Cells by Enriching RAN and HSBP1 mRNA and Up-regulating its Protein
            Expression.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 8649)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 8649)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 8649)
  AUTHORS   Enguix-Riego,M.D.V., Cacicedo,J., Delgado Leon,B.D., Nieto-Guerrero
            Gomez,J.M., Herrero Rivera,D., Perez,M., Praena-Fernandez,J.M.,
            Sanchez Carmona,G., Rivin Del Campo,E., Ortiz Gordillo,M.J. and
            Lopez Guerra,J.L.
  TITLE     The single nucleotide variant rs2868371 associates with the risk of
            mortality in non-small cell lung cancer patients: A multicenter
            prospective validation
  JOURNAL   Radiother Oncol 136, 29-36 (2019)
   PUBMED   31015126
  REMARK    GeneRIF: The functional HSPB1 rs2868371 promoter variant may affect
            lung cancer survival by regulation of HSPB1 expression levels
            through glucocorticoid receptor interaction.
REFERENCE   6  (bases 1 to 8649)
  AUTHORS   Yashin,D.V., Dukhanina,E.A., Kabanova,O.D., Romanova,E.A.,
            Lukyanova,T.I., Tonevitskii,A.G., Raynes,D.A., Gnuchev,N.V.,
            Guerriero,V., Georgiev,G.P. and Sashchenko,L.P.
  TITLE     The heat shock-binding protein (HspBP1) protects cells against the
            cytotoxic action of the Tag7-Hsp70 complex
  JOURNAL   J Biol Chem 286 (12), 10258-10264 (2011)
   PUBMED   21247889
  REMARK    GeneRIF: HspBP1 inhibited the cytotoxic activity of the Tag7-Hsp70
            complex secreted by lymphocytes. HspBP1
REFERENCE   7  (bases 1 to 8649)
  AUTHORS   Liu,X., Xu,L., Liu,Y., Tong,X., Zhu,G., Zhang,X.C., Li,X. and
            Rao,Z.
  TITLE     Crystal structure of the hexamer of human heat shock factor binding
            protein 1
  JOURNAL   Proteins 75 (1), 1-11 (2009)
   PUBMED   18767159
  REMARK    GeneRIF: This study reports the crystal structure of a
            degradation-resistant fragment of HSBP1. A continuous, long-helix
            conformation of HSBP1 assembles into a coiled-coil three-helix
            bundle and subsequently into an elongated, symmetrical hexamer.
REFERENCE   8  (bases 1 to 8649)
  AUTHORS   Tai,L.J., McFall,S.M., Huang,K., Demeler,B., Fox,S.G., Brubaker,K.,
            Radhakrishnan,I. and Morimoto,R.I.
  TITLE     Structure-function analysis of the heat shock factor-binding
            protein reveals a protein composed solely of a highly conserved and
            dynamic coiled-coil trimerization domain
  JOURNAL   J Biol Chem 277 (1), 735-745 (2002)
   PUBMED   11679589
  REMARK    GeneRIF: HSBP likely mediates its function through its
            trimerization domain.
REFERENCE   9  (bases 1 to 8649)
  AUTHORS   Satyal,S.H., Chen,D., Fox,S.G., Kramer,J.M. and Morimoto,R.I.
  TITLE     Negative regulation of the heat shock transcriptional response by
            HSBP1
  JOURNAL   Genes Dev 12 (13), 1962-1974 (1998)
   PUBMED   9649501
REFERENCE   10 (bases 1 to 8649)
  AUTHORS   Morimoto,R.I., Kline,M.P., Bimston,D.N. and Cotto,J.J.
  TITLE     The heat-shock response: regulation and function of heat-shock
            proteins and molecular chaperones
  JOURNAL   Essays Biochem 32, 17-29 (1997)
   PUBMED   9493008
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC009119.10, BC007515.2,
            AK172732.1 and DB519225.1.
            
            On May 17, 2019 this sequence version replaced NM_001537.3.
            
            Summary: The heat-shock response is elicited by exposure of cells
            to thermal and chemical stress and through the activation of HSFs
            (heat shock factors) results in the elevated expression of
            heat-shock induced genes.  Heat shock factor binding protein 1
            (HSBP1), is a 76-amino-acid protein that binds to heat shock factor
            1(HSF1), which is a transcription factor involved in the HS
            response.  During HS response, HSF1 undergoes conformational
            transition from an inert non-DNA-binding monomer to active
            functional trimers. HSBP1 is nuclear-localized and interacts  with
            the active trimeric state of HSF1 to negatively regulate HSF1
            DNA-binding activity. Overexpression of HSBP1 in mammalian cells
            represses the transactivation activity of HSF1. When overexpressed
            in C.elegans HSBP1 has severe effects on survival of the animals
            after thermal and chemical stress consistent with a role of HSBP1
            as a negative regulator of heat shock response. [provided by
            RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803616.38835.1,
                                           SRR1803613.41892.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000433866.7/ ENSP00000392896.2
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-53                AC009119.10        37477-37529
            54-566              BC007515.2         1-513
            567-1504            AK172732.1         507-1444
            1505-1914           DB519225.1         68-477
            1915-8649           AC009119.10        42502-49236
FEATURES             Location/Qualifiers
     source          1..8649
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16q23.3"
     gene            1..8649
                     /gene="HSBP1"
                     /gene_synonym="NPC-A-13"
                     /note="heat shock factor binding protein 1"
                     /db_xref="GeneID:3281"
                     /db_xref="HGNC:HGNC:5203"
                     /db_xref="MIM:604553"
     exon            1..144
                     /gene="HSBP1"
                     /gene_synonym="NPC-A-13"
                     /inference="alignment:Splign:2.1.0"
     CDS             100..330
                     /gene="HSBP1"
                     /gene_synonym="NPC-A-13"
                     /note="nasopharyngeal carcinoma-associated antigen 13"
                     /codon_start=1
                     /product="heat shock factor-binding protein 1"
                     /protein_id="NP_001528.1"
                     /db_xref="CCDS:CCDS45534.1"
                     /db_xref="GeneID:3281"
                     /db_xref="HGNC:HGNC:5203"
                     /db_xref="MIM:604553"
                     /translation="MAETDPKTVQDLTSVVQTLLQQMQDKFQTMSDQIIGRIDDMSSR
                     IDDLEKNIADLMTQAGVEELESENKIPATQKS"
     exon            145..211
                     /gene="HSBP1"
                     /gene_synonym="NPC-A-13"
                     /inference="alignment:Splign:2.1.0"
     exon            212..332
                     /gene="HSBP1"
                     /gene_synonym="NPC-A-13"
                     /inference="alignment:Splign:2.1.0"
     exon            333..8649
                     /gene="HSBP1"
                     /gene_synonym="NPC-A-13"
                     /inference="alignment:Splign:2.1.0"
     regulatory      548..553
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HSBP1"
                     /gene_synonym="NPC-A-13"
                     /note="hexamer: ATTAAA"
     regulatory      555..560
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HSBP1"
                     /gene_synonym="NPC-A-13"
                     /note="hexamer: ACTAAA"
     polyA_site      578
                     /gene="HSBP1"
                     /gene_synonym="NPC-A-13"
                     /note="major polyA site"
     polyA_site      581
                     /gene="HSBP1"
                     /gene_synonym="NPC-A-13"
     regulatory      694..699
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HSBP1"
                     /gene_synonym="NPC-A-13"
                     /note="hexamer: AGTAAA"
     polyA_site      707
                     /gene="HSBP1"
                     /gene_synonym="NPC-A-13"
     regulatory      1881..1886
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HSBP1"
                     /gene_synonym="NPC-A-13"
                     /note="hexamer: AATAAA"
     polyA_site      1905
                     /gene="HSBP1"
                     /gene_synonym="NPC-A-13"
     regulatory      3579..3584
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HSBP1"
                     /gene_synonym="NPC-A-13"
                     /note="hexamer: ATTAAA"
     regulatory      3603..3608
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HSBP1"
                     /gene_synonym="NPC-A-13"
                     /note="hexamer: AATAAA"
     polyA_site      3608
                     /gene="HSBP1"
                     /gene_synonym="NPC-A-13"
     polyA_site      3622
                     /gene="HSBP1"
                     /gene_synonym="NPC-A-13"
     regulatory      7451..7456
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HSBP1"
                     /gene_synonym="NPC-A-13"
                     /note="hexamer: AATAAA"
     polyA_site      7482
                     /gene="HSBP1"
                     /gene_synonym="NPC-A-13"
     regulatory      8177..8182
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HSBP1"
                     /gene_synonym="NPC-A-13"
                     /note="hexamer: AATAAA"
     polyA_site      8197
                     /gene="HSBP1"
                     /gene_synonym="NPC-A-13"
ORIGIN      
        1 agtctcgtcg cgagaagcag cggcccgggg cgactgagcg gacaaacgga agtgtaggtt
       61 acggtctgag acatcaccgc caagctgggc atcggggaga tggccgagac tgaccccaag
      121 accgtgcagg acctcacctc ggtggtgcag acactcctgc agcagatgca agataaattt
      181 cagaccatgt ctgaccagat cattgggaga attgatgata tgagtagtcg cattgatgat
      241 ctggaaaaga atatcgcgga cctcatgaca caggctgggg tggaagaact ggaaagtgaa
      301 aacaagatac ctgccacgca aaagagttga aggttgctaa taatttatac tggaatctgg
      361 catttttcca agccaagaga agatcgaatg gctttttgca gctaactact atgtgtagac
      421 aggttttata ttataaagta tgcattctta tcacctagta tatagttagt ttgtagagtg
      481 atttcccccc agtttcttga acatggtatc ttcacatctt ggaccttggt cagttgtgct
      541 attcattatt aaacactaaa actttggcgg ttcttgcata acattgtcag attttttagt
      601 gtatttctgt gaagtcattt tttttcttgt cattcctttt gtagtagttg ctgtttggat
      661 aaaagttgat gtgtgatttt ttattaaaca aatagtaaac ccttcaatta tagttagtct
      721 tggtgaagta agatgtttgt agactttaga gttctttaat tcttggcaca acgtgactgt
      781 tgagctaaca ccaaatagtg tgttggcaat acttttcaaa tggctgaaaa cacctaaaaa
      841 ttgttcattc agaaatatct gtcactgctc tgttgccaaa actcagaata gaacttagac
      901 gtatgtctga gtccctgaga tcacatgcta aagtcgatga aaagtaacca ctgccactgt
      961 cttgtgtcag aacttttaca gtacagaaaa taacagaata gccttctgta atgaggcgtt
     1021 tgttagagtt ttgcatgaga ttctaatact tcagtaggac cctacctacg tggttcatct
     1081 acaatggtta ccataaaaaa tctggcagga ttttaaaact caatcagtct ttcctttgag
     1141 ctagtgactt gaaaagaaag agagaaggaa aagagaccat attaagtcca tgccagttgc
     1201 ttggctagaa tatgatcaac gacttgtagt agactcaagt ttttaaaaaa cactatttta
     1261 cttaaactgt ttcttatcta aattcttgca gagtgtcaat gttatcattg attatagaag
     1321 acagggataa tacctttatc tctggccact caaaaatgca gtgccaggag tgctaaacct
     1381 agaggccaat actgatgacc tggaaggtga tccatatgat tgtcaccaca aagtgctttt
     1441 acacaaaaac ttgaaaattt gaaaaacatg atttttttaa gtttctcatc tcaccagtct
     1501 tggtgtttat attgcaaatc tatcaaagta agaaataatt tgtgctgtat acaaattaca
     1561 tggggaacat aaaggagtga gatccttctg tgataaaatg aattcaccac tctggttacc
     1621 caactacaga acctcctttg atcaggccag taggttgtga tgcaggctgg agcccccgaa
     1681 tgccccacac acactgcagc attgaccaga ccatccgaaa cctgcgtccc tggtgatgtt
     1741 ctcaagcctc ggaagtggca aatggaaatg atatggccgg ttgcggttgt aggagagttg
     1801 tgacttaggc aggagtcgac ctcctcaagt aatggaacga tttcaaaggc aggctgccct
     1861 gaccaaaaat atctgccatg aataaaggtg cctgaaatcc tgctatgaag cttcctttgt
     1921 gtcaaaaatc tgtttggttt tcagatggaa atgcagaaat cacattagag tatcaactaa
     1981 aacctctgga cggattaagt acaagtagcc caaggcaaag caggagaggg ggtgttgggg
     2041 gaggttgcct gctggaaggg gaagctgagg agcgggagga gggcttgcca agactggcat
     2101 tgcaggagac ttgtctcagg tgtgttccca gaactcttgc ccctggagat gctgcaggaa
     2161 ggaaggggtt atggtcactt gggtttagac gacattctgt gaacctcatg aagaaattag
     2221 catattaaag actctgaaag tagagcagag aactcagctt taacctgctc acctgctagt
     2281 tttcgaggtc tcgctctgtt gcccaggctg gagtgcagtg gcacagtcat agctcacttg
     2341 gagcctgaag ctcctgggct caggcagtcc tcctgcctca gcctcccaag tagctaatat
     2401 tacaggttcg tgccaccgca cctgggtctt gctgtgttgc ccaggctggt ctagaactcc
     2461 taggctcaag cagtcctgtc tcagcctccc aaagtgctgg gattacaggc tggagccacc
     2521 atgcctgtcc aagggtcttt tttatactat atgaagtatt ccttctccag ctattcttgt
     2581 tcgtttacca gccagtgaag attctcttga gggctgaaca atacagatga gtatgggggc
     2641 cctcgcacca aggaaaacag cgttacattt aattaagtgc cctgtctcaa accccaaggc
     2701 cccacagctt cttcatttgg cccctagttc atcctgcttt cctttgagcc acaggatttt
     2761 aaaatatttc aatcttattt tcatatcaat tttttctttg tttcactaca agtacagtca
     2821 ttgtttaacc ctatcaggac taacacaata tttcacgtaa ctaagagtta atttgccctt
     2881 gtgagtcctt ggaatttaag ccccacacac acacacttag agcactgttg ctatgggaac
     2941 atagagtcct ccaaacaaaa gtgaactttt tcaacatgct gtttgtaaat ggaagccttc
     3001 ccaagcagac actgtgttcc tcttttattt tgtattttgc acattatata ttttgattag
     3061 attaaaatgg aggaatttgc aggatgtgtt gtcatatcta gccctgaaag ttaactggtt
     3121 acagttacca aatgttcaca gctagcgcca ttgaaagcat tgactgtaaa ccgctgtact
     3181 gtgctaagcc ttttgtttgc actgtttggc ttaatcctca caacaacact gagaggtttt
     3241 catttgatga gtcatattta aagaaaagga acccaagacg gggagtcagg ggcacaaggt
     3301 cacagctggt aatgatgacc ctggaatgtc ctcacagctg cacacctgca ccgctcacaa
     3361 acacagctga ccgtctcaca gctgcacacc tgcaccgctc acgagcacag ctgaccgtct
     3421 cacagctgca cacctgcact gctcacgaac acagctgatc gtctgcctct tcgtgtgagc
     3481 tgagctccga atctcagctg tccaggctgt ctggaaatca tcagccccac tggctggatc
     3541 tcaaaattta ctttcaaaag gctgatggag tgttttcaat taaacatgac ctaaaattaa
     3601 gtaataaaca cactggatgt gagtttttat ttggaattta aactattcag agctggtaga
     3661 aaatgtgtgc tcctggatca gtgccttttt tctcctaaat cagttgagaa caacaacaga
     3721 aacaaatgac attcctcaaa catttgccca gagcacaacc acggggaata tgaaccacag
     3781 gttttcacat tggttttcca tgcctatacc tgtcctgtct cccacaaaaa ttattgcttg
     3841 aggcccaacc cgggggataa aaagacaccc ccccgccact ccgtgaccta ttagcttacc
     3901 atcctattgg atttcactgt tttttgtttt gtggtgtttt ttttttattg caacgaagac
     3961 ttttaaatgg actcatgaag aagccatcac ctggaatctt aatcttgcat taattttaag
     4021 tgtctgattt tagctgcagc cacttgagaa cttttcaatg aatgaaaatg agggagggga
     4081 gatgcaagtc actgcccaaa aacaaaacca gaggaattgt gattgaacat tgcacttatc
     4141 aaccctacaa attaatatgg aaataggaaa agggtttgtt tcaacaagtc aggatgacag
     4201 tattttaaaa aagaaaaaaa ggccagcctg ggcaatatac tgagactcca tctctacaaa
     4261 aaagtagaaa aattagccca gagtgtggtg gcacacatct gtgctcccag ctactcagga
     4321 ggctgaggtg ggaggatcat ttgagcccag gaagtcgagg ttgcagtgag ctgtgatcgg
     4381 acgactccac tccagccggg gtgacagagc aagaccctgt ctcaaaaaaa aaaaaaaagg
     4441 gcggatccta acgcttggtc ctcacacctg aggctctcac taagaggtgg ctggaagctc
     4501 ctcagaccgc tgcctagggt tcgagtgatc ctgaaagctg tacaacatca gcctctgtgg
     4561 gatccctgag aatgatgaaa tgtaatatgt ggctgtctca tcaacagagc ttcattttac
     4621 caaagcatat gtagacacca catacatgaa ctacatacct atcgtgtaga tgctgccctg
     4681 cctcataaga acactcttga ctgtcctgct tcacaataat aatcttcgca ataatgatct
     4741 aataatggtc aaattctgtg tctctaccgt agaaaacaga atttgaccat ttagtcacgt
     4801 tggtcttgca aaatgtccac cttccaaaag agaacagtat tgcttcccaa taattaagaa
     4861 tagtctggat tcaggatcca gcagcttaga agcgtttagc taccaatagc aaaaatcagc
     4921 ccttttaaca atggtgggat gtattatttc acatggcaag aagtcccaag ggagggcagc
     4981 tctggggcga gtaaattctg cacctcagta aaactaagag caagacccgg ccgggcgcag
     5041 tggctcatgc ctgtaatccc agcactttgg gaggccgagg tgggtggatc acctgaggtc
     5101 aggagttcta aaccagcctg gccaacatgg cgaaaccccc gtctctacta aaaatacaaa
     5161 aattagccag gcgtggtggt gggcgcttgt aatcccagtt gctcgggagg ctgagggagg
     5221 aggatcgctt gaactcagga ggtggaagtt gcagtgagcc gagatcgtgc cactgcactc
     5281 cagcctgggt aacagagcga gactccatct caaaaaataa aaaacaaaaa aataaataaa
     5341 aaataaaaag acccaacccc agatttgttc cacctgggtg catccggtca gagaatgcct
     5401 ttcttcttcc cttttgtctc ctaagagtga agaaaatgtt tctttgaagg tgtttccacc
     5461 ccactcctgt gcctaaaatg atcctggcaa acaggacccg catgataggt tgagccatgt
     5521 ggttcttgtt tttttgttgt cacaactcag gttgggggtg ttactggcat caagagtgta
     5581 gaaaccaggg atgctgcgtc aacaccctac catgcacagg acaaccctca ccccaaagag
     5641 tggcacagcc ccaagtgtca acagtgctga ggttgaggaa tcccagctta acccattcat
     5701 gagaccacct gagcctggtg taacggttct gctgatagtt tccggaccct cagaggtgga
     5761 gacataagag aacaatacca gggttccatc agaaaggaaa tcagaatggc tatcgggtgg
     5821 acaacctaca gtgtttgcta cagaccttct agatgtaact tctagattat aaacactggt
     5881 aacactggag atggttaaat cggaattagc tgactgtcaa ccttggtggc acattagaat
     5941 cacctgggga gcagttcaaa ggcccaaaac tgggcagcac cacagaaccc tcacatcaga
     6001 atccccatgg gtgggcccag acagcagtgt tcatcttaga ctccagtgtg cagtcgaggt
     6061 cgagaactgc attcaagaag gcctgtctac tgcgaaccgt ccgatgatgt aattcaccaa
     6121 gttccatggg attccgccac gcccgtgtca acccagctga cacgtccaac ttggacaatc
     6181 agggctttga cccccgttac ctggcattca attcaatcca tgtgtcacgc atgccccaac
     6241 aatttgtgtc ccaactgttt gaatcaaagg atttctgatt ccctgatggg tgatgactca
     6301 gtaaccggat tcagcttcag atctgcctga catttttcct gcctgtcctg ggccaggtat
     6361 ggcgctagct gctttcacct gtatcatctc attcactgct cagaggctct cgaaagctgt
     6421 tgacattatc aaaaagtttt ccagctggaa ttcatctccc tctaatcagc cttcaaaacc
     6481 tccatttgta aaccattgct gtagttttat tacctcttac cttggtcttt acaagttgct
     6541 gtcaaaaccc agtaagtgtt tacagaaaga aactttacat attaaatatc ttaaaggtct
     6601 tttatgcaga atatgcagtt aattttgatg ggctatggat aatcatcaga agagccattt
     6661 ctggaaatgt aaataatatg gctgtttctt ttaacagtgc attctcctga aaacataaga
     6721 ccatttgact gattctgctc cagaatctta ttgaggcaaa ggactggacc gaattattca
     6781 tggaacagaa gcctaggact ggtaagtcca ggaaatgctg caaaacaatg ccctcaaaag
     6841 aaatcagcct ttccctttga taggtatctc tcctgcaagc caggctcagg tgcgcctcaa
     6901 ttagaacctg tgttcgctgg ggccataatt aagaatctta aagggaaaga atgttttgaa
     6961 agccatctgt caaaagtacg cagtcttgct tgtagaattt tagaggttca tggttaccgt
     7021 gggtcggcac tgggtctgtg agccctcatc cccagggctc ggtttcctat cagaatcctt
     7081 gtctggaagg ttgattatat cagatgcgca ttttgccagc atcctcagaa ggagatgcga
     7141 ggaagattct tttcctgggc cactctcatt ttctcaaact tggacaagtc ttaacaataa
     7201 tctgtgaccc aactttgtct tccccatgat gaaaaacaga ccccatagaa actctctggc
     7261 acaaaaactc tggaatcact gaccccagac attgttccct gccatccact ctgccagaca
     7321 tttcttggat ttattcaagc tgacttgtat tccagaacaa ttgtggacaa gatttgctag
     7381 taatgcttcc aacaagttac cgtttagcaa gttcagaact tgaagaatcc cttttatcct
     7441 aacgctgact aataaactgg tgagaactgt ttgaaatacc cataagtcaa tggaggtcgt
     7501 aatttgcttt tatgctgtat taaacaccac tgagatgtta aggtctttct tgggaaccac
     7561 cgtgatgttc tcttctggac tcttcgtaac cccttcagca gacgaccctg tatctggtct
     7621 tggttctgaa aggtttgcag cggcacctaa gtcactgaag ttctggcaga attcattctc
     7681 ctgctctcta ctgggacttg ttttccataa ttggtctgag aacaacagat ttttttcttt
     7741 tttgcctcgg atttcaacaa aacatacatg gaaagaatat tttcttcctt ggagacattt
     7801 cccatagggc agtggttctt aaaatacgtt cctgaaccgg cagcatcaga atcccctggg
     7861 aacgtgctgg aaatgtagat ccccaggccc tacctagccc tactgaatca gagaccctgg
     7921 gagggaggtc cagcagcctg ggttttaaca catcctccag gcgattctga gagtgagaga
     7981 gccagggcct tcgggcatca accttggggc gagggtgttg ggttgcccaa caccctttag
     8041 tctaatggct ttagggtcaa atatgtctaa accaagaagc cccatggggc actgattagc
     8101 atgcaggtcc ccaggccccc tttttgtaga tcctgattca gaatatctgg gacgggggct
     8161 ggagattcat atttttaata aattccctca tttattattt ttgtgtctta tttcccatac
     8221 acaaaaacac tgcgtagaga aatgggagtg tgggcgatgc tcccatgctg ggagtctcag
     8281 gcctgggcta gtcctaaaat aattgaaaag gaattgattc ttaattaccc agaacgtcca
     8341 cgctctagga cctagtgtga cctcttctgc cttctgaacc gaggagtcca cccctaagcc
     8401 cgaggaactg agcgtgaaga gccctgacgc catgaagact tgaatcactt taaagcaatt
     8461 tgcttcttat taaaatgtac ttcagtaaaa aatcatttca actcttaagc cacaaaagga
     8521 tatccaatca tttaaagctt cctgagtgtt tcggttgatt aaaaagcttt taaagagcat
     8581 agtaaatatt ttaccagaag gataagttat tcagtatgga gattatttat taaaaactag
     8641 taatttatt
//
