LOCUS       NM_153456               8536 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens heparan sulfate 6-O-sulfotransferase 3 (HS6ST3), mRNA.
ACCESSION   NM_153456 NM_205551 XM_926275 XM_931159 XM_941593 XM_945293
VERSION     NM_153456.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8536)
  AUTHORS   Iravani,O., Bay,B.H. and Yip,G.W.
  TITLE     Silencing HS6ST3 inhibits growth and progression of breast cancer
            cells through suppressing IGF1R and inducing XAF1
  JOURNAL   Exp Cell Res 350 (2), 380-389 (2017)
   PUBMED   28017727
  REMARK    GeneRIF: Silencing HS6ST3 diminished tumor cell growth and
            progression in breast cancer cells.  Silencing HS6ST3
            down-regulated IGF1R and up-regulated XAF1.
REFERENCE   2  (bases 1 to 8536)
  AUTHORS   Cox,A.J., Hugenschmidt,C.E., Raffield,L.M., Langefeld,C.D.,
            Freedman,B.I., Williamson,J.D., Hsu,F.C. and Bowden,D.W.
  TITLE     Heritability and genetic association analysis of cognition in the
            Diabetes Heart Study
  JOURNAL   Neurobiol Aging 35 (8), 1958 (2014)
   PUBMED   24684796
REFERENCE   3  (bases 1 to 8536)
  AUTHORS   Wang,K.S., Wang,L., Liu,X. and Zeng,M.
  TITLE     Association of HS6ST3 gene polymorphisms with obesity and
            triglycerides: gene x gender interaction
  JOURNAL   J Genet 92 (3), 395-402 (2013)
   PUBMED   24371161
  REMARK    GeneRIF: Single-nucleotide polymorphisms within the HS6ST3 gene are
            associated with obesity and triglyceride levels. [Meta-analysis]
REFERENCE   4  (bases 1 to 8536)
  AUTHORS   Berndt,S.I., Gustafsson,S., Magi,R., Ganna,A., Wheeler,E.,
            Feitosa,M.F., Justice,A.E., Monda,K.L., Croteau-Chonka,D.C.,
            Day,F.R., Esko,T., Fall,T., Ferreira,T., Gentilini,D.,
            Jackson,A.U., Luan,J., Randall,J.C., Vedantam,S., Willer,C.J.,
            Winkler,T.W., Wood,A.R., Workalemahu,T., Hu,Y.J., Lee,S.H.,
            Liang,L., Lin,D.Y., Min,J.L., Neale,B.M., Thorleifsson,G., Yang,J.,
            Albrecht,E., Amin,N., Bragg-Gresham,J.L., Cadby,G., den Heijer,M.,
            Eklund,N., Fischer,K., Goel,A., Hottenga,J.J., Huffman,J.E.,
            Jarick,I., Johansson,A., Johnson,T., Kanoni,S., Kleber,M.E.,
            Konig,I.R., Kristiansson,K., Kutalik,Z., Lamina,C., Lecoeur,C.,
            Li,G., Mangino,M., McArdle,W.L., Medina-Gomez,C.,
            Muller-Nurasyid,M., Ngwa,J.S., Nolte,I.M., Paternoster,L.,
            Pechlivanis,S., Perola,M., Peters,M.J., Preuss,M., Rose,L.M.,
            Shi,J., Shungin,D., Smith,A.V., Strawbridge,R.J., Surakka,I.,
            Teumer,A., Trip,M.D., Tyrer,J., Van Vliet-Ostaptchouk,J.V.,
            Vandenput,L., Waite,L.L., Zhao,J.H., Absher,D., Asselbergs,F.W.,
            Atalay,M., Attwood,A.P., Balmforth,A.J., Basart,H., Beilby,J.,
            Bonnycastle,L.L., Brambilla,P., Bruinenberg,M., Campbell,H.,
            Chasman,D.I., Chines,P.S., Collins,F.S., Connell,J.M.,
            Cookson,W.O., de Faire,U., de Vegt,F., Dei,M., Dimitriou,M.,
            Edkins,S., Estrada,K., Evans,D.M., Farrall,M., Ferrario,M.M.,
            Ferrieres,J., Franke,L., Frau,F., Gejman,P.V., Grallert,H.,
            Gronberg,H., Gudnason,V., Hall,A.S., Hall,P., Hartikainen,A.L.,
            Hayward,C., Heard-Costa,N.L., Heath,A.C., Hebebrand,J., Homuth,G.,
            Hu,F.B., Hunt,S.E., Hypponen,E., Iribarren,C., Jacobs,K.B.,
            Jansson,J.O., Jula,A., Kahonen,M., Kathiresan,S., Kee,F.,
            Khaw,K.T., Kivimaki,M., Koenig,W., Kraja,A.T., Kumari,M.,
            Kuulasmaa,K., Kuusisto,J., Laitinen,J.H., Lakka,T.A.,
            Langenberg,C., Launer,L.J., Lind,L., Lindstrom,J., Liu,J.,
            Liuzzi,A., Lokki,M.L., Lorentzon,M., Madden,P.A., Magnusson,P.K.,
            Manunta,P., Marek,D., Marz,W., Mateo Leach,I., McKnight,B.,
            Medland,S.E., Mihailov,E., Milani,L., Montgomery,G.W., Mooser,V.,
            Muhleisen,T.W., Munroe,P.B., Musk,A.W., Narisu,N., Navis,G.,
            Nicholson,G., Nohr,E.A., Ong,K.K., Oostra,B.A., Palmer,C.N.,
            Palotie,A., Peden,J.F., Pedersen,N., Peters,A., Polasek,O.,
            Pouta,A., Pramstaller,P.P., Prokopenko,I., Putter,C.,
            Radhakrishnan,A., Raitakari,O., Rendon,A., Rivadeneira,F.,
            Rudan,I., Saaristo,T.E., Sambrook,J.G., Sanders,A.R., Sanna,S.,
            Saramies,J., Schipf,S., Schreiber,S., Schunkert,H., Shin,S.Y.,
            Signorini,S., Sinisalo,J., Skrobek,B., Soranzo,N., Stancakova,A.,
            Stark,K., Stephens,J.C., Stirrups,K., Stolk,R.P., Stumvoll,M.,
            Swift,A.J., Theodoraki,E.V., Thorand,B., Tregouet,D.A., Tremoli,E.,
            Van der Klauw,M.M., van Meurs,J.B., Vermeulen,S.H., Viikari,J.,
            Virtamo,J., Vitart,V., Waeber,G., Wang,Z., Widen,E., Wild,S.H.,
            Willemsen,G., Winkelmann,B.R., Witteman,J.C., Wolffenbuttel,B.H.,
            Wong,A., Wright,A.F., Zillikens,M.C., Amouyel,P., Boehm,B.O.,
            Boerwinkle,E., Boomsma,D.I., Caulfield,M.J., Chanock,S.J.,
            Cupples,L.A., Cusi,D., Dedoussis,G.V., Erdmann,J., Eriksson,J.G.,
            Franks,P.W., Froguel,P., Gieger,C., Gyllensten,U., Hamsten,A.,
            Harris,T.B., Hengstenberg,C., Hicks,A.A., Hingorani,A., Hinney,A.,
            Hofman,A., Hovingh,K.G., Hveem,K., Illig,T., Jarvelin,M.R.,
            Jockel,K.H., Keinanen-Kiukaanniemi,S.M., Kiemeney,L.A., Kuh,D.,
            Laakso,M., Lehtimaki,T., Levinson,D.F., Martin,N.G., Metspalu,A.,
            Morris,A.D., Nieminen,M.S., Njolstad,I., Ohlsson,C.,
            Oldehinkel,A.J., Ouwehand,W.H., Palmer,L.J., Penninx,B., Power,C.,
            Province,M.A., Psaty,B.M., Qi,L., Rauramaa,R., Ridker,P.M.,
            Ripatti,S., Salomaa,V., Samani,N.J., Snieder,H., Sorensen,T.I.,
            Spector,T.D., Stefansson,K., Tonjes,A., Tuomilehto,J.,
            Uitterlinden,A.G., Uusitupa,M., van der Harst,P., Vollenweider,P.,
            Wallaschofski,H., Wareham,N.J., Watkins,H., Wichmann,H.E.,
            Wilson,J.F., Abecasis,G.R., Assimes,T.L., Barroso,I., Boehnke,M.,
            Borecki,I.B., Deloukas,P., Fox,C.S., Frayling,T., Groop,L.C.,
            Haritunian,T., Heid,I.M., Hunter,D., Kaplan,R.C., Karpe,F.,
            Moffatt,M.F., Mohlke,K.L., O'Connell,J.R., Pawitan,Y., Schadt,E.E.,
            Schlessinger,D., Steinthorsdottir,V., Strachan,D.P.,
            Thorsteinsdottir,U., van Duijn,C.M., Visscher,P.M., Di Blasio,A.M.,
            Hirschhorn,J.N., Lindgren,C.M., Morris,A.P., Meyre,D., Scherag,A.,
            McCarthy,M.I., Speliotes,E.K., North,K.E., Loos,R.J. and
            Ingelsson,E.
  TITLE     Genome-wide meta-analysis identifies 11 new loci for anthropometric
            traits and provides insights into genetic architecture
  JOURNAL   Nat Genet 45 (5), 501-512 (2013)
   PUBMED   23563607
REFERENCE   5  (bases 1 to 8536)
  AUTHORS   Huang,Y.C., Lin,J.M., Lin,H.J., Chen,C.C., Chen,S.Y., Tsai,C.H. and
            Tsai,F.J.
  TITLE     Genome-wide association study of diabetic retinopathy in a
            Taiwanese population
  JOURNAL   Ophthalmology 118 (4), 642-648 (2011)
   PUBMED   21310492
REFERENCE   6  (bases 1 to 8536)
  AUTHORS   Jiang,Z., Michal,J.J., Wu,X.L., Pan,Z. and MacNeil,M.D.
  TITLE     The heparan and heparin metabolism pathway is involved in
            regulation of fatty acid composition
  JOURNAL   Int J Biol Sci 7 (5), 659-663 (2011)
   PUBMED   21647334
REFERENCE   7  (bases 1 to 8536)
  AUTHORS   Nagai,N., Habuchi,H., Esko,J.D. and Kimata,K.
  TITLE     Stem domains of heparan sulfate 6-O-sulfotransferase are required
            for Golgi localization, oligomer formation and enzyme activity
  JOURNAL   J Cell Sci 117 (Pt 15), 3331-3341 (2004)
   PUBMED   15226404
REFERENCE   8  (bases 1 to 8536)
  AUTHORS   Christian,S.L., McDonough,J., Liu Cy,C.Y., Shaikh,S., Vlamakis,V.,
            Badner,J.A., Chakravarti,A. and Gershon,E.S.
  TITLE     An evaluation of the assembly of an approximately 15-Mb region on
            human chromosome 13q32-q33 linked to bipolar disorder and
            schizophrenia
  JOURNAL   Genomics 79 (5), 635-656 (2002)
   PUBMED   11991713
REFERENCE   9  (bases 1 to 8536)
  AUTHORS   Habuchi,H., Tanaka,M., Habuchi,O., Yoshida,K., Suzuki,H., Ban,K.
            and Kimata,K.
  TITLE     The occurrence of three isoforms of heparan sulfate
            6-O-sulfotransferase having different specificities for hexuronic
            acid adjacent to the targeted N-sulfoglucosamine
  JOURNAL   J Biol Chem 275 (4), 2859-2868 (2000)
   PUBMED   10644753
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL158192.15, AL138816.12 and AL353581.14.
            
            On Jul 8, 2020 this sequence version replaced NM_153456.3.
            
            Summary: Heparan sulfate (HS) sulfotransferases, such as HS6ST3,
            modify HS to generate structures required for interactions between
            HS and a variety of proteins. These interactions are implicated in
            proliferation and differentiation, adhesion, migration,
            inflammation, blood coagulation, and other diverse processes
            (Habuchi et al., 2000 [PubMed 10644753]).[supplied by OMIM, Mar
            2008].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK126947.1, CR749677.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968189, SAMEA1970526
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000376705.4/ ENSP00000365895.2
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1463              AL158192.15        78738-80200
            1464-4800           AL138816.12        139084-142420
            4801-8536           AL353581.14        101-3836
FEATURES             Location/Qualifiers
     source          1..8536
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="13"
                     /map="13q32.1"
     gene            1..8536
                     /gene="HS6ST3"
                     /gene_synonym="HS6ST-3"
                     /note="heparan sulfate 6-O-sulfotransferase 3"
                     /db_xref="GeneID:266722"
                     /db_xref="HGNC:HGNC:19134"
                     /db_xref="MIM:609401"
     exon            1..1463
                     /gene="HS6ST3"
                     /gene_synonym="HS6ST-3"
                     /inference="alignment:Splign:2.1.0"
     CDS             757..2172
                     /gene="HS6ST3"
                     /gene_synonym="HS6ST-3"
                     /EC_number="2.8.2.-"
                     /codon_start=1
                     /product="heparan-sulfate 6-O-sulfotransferase 3"
                     /protein_id="NP_703157.2"
                     /db_xref="CCDS:CCDS9481.1"
                     /db_xref="GeneID:266722"
                     /db_xref="HGNC:HGNC:19134"
                     /db_xref="MIM:609401"
                     /translation="MDERFNKWLLTPVLTLLFVVIMYQYVSPSCTSSCTNFGEQPRAG
                     EAGPPAVPGPARRAQAPPEEWERRPQLPPPPRGPPEGPRGAAAPEEEDEEPGDPREGE
                     EEEEEDEPDPEAPENGSLPRFVPRFNFSLKDLTRFVDFNIKGRDVIVFLHIQKTGGTT
                     FGRHLVKNIRLEQPCSCKAGQKKCTCHRPGKKETWLFSRFSTGWSCGLHADWTELTNC
                     VPAIMEKKDCPRNHSHTRNFYYITMLRDPVSRYLSEWKHVQRGATWKTSLHMCDGRSP
                     TPDELPTCYPGDDWSGVSLREFMDCTYNLANNRQVRMLADLSLVGCYNLTFMNESERN
                     TILLQSAKNNLKNMAFFGLTEFQRKTQFLFERTFNLKFISPFTQFNITRASNVEINEG
                     ARQRIEDLNFLDMQLYEYAKDLFQQRYHHTKQLEHQRDRQKRREERRLQREHRDHQWP
                     KEDGAAEGTVTEDYNSQVVRW"
     exon            1464..8536
                     /gene="HS6ST3"
                     /gene_synonym="HS6ST-3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8510..8515
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HS6ST3"
                     /gene_synonym="HS6ST-3"
                     /note="hexamer: ATTAAA"
     polyA_site      8536
                     /gene="HS6ST3"
                     /gene_synonym="HS6ST-3"
                     /note="major polyA site"
ORIGIN      
        1 acaccggctg gcgggttgtg gcggtgccag cctgtgtgtg cgagtgtgtc tgcgtgcctg
       61 cgcctgggcg gacagcccgg agcggcagtg cgactcgccg ggagaggcgt ctccgcagag
      121 gcgcctcgcc cgctgccgct gcgctgcggg ggccgctctg caaacttgcg gcgagcgccg
      181 tcagccctcg cggctccaca gccccgcgac ctgccggcaa aggcgccggg cgcgcgtgcc
      241 gggcgcggtg cccgcggccg gccggggcgg cgggagcggg ccgcgccttc gcccttggcc
      301 ggcgccctgg cgagcctcat gcagccccgg cgctcgcggc cgcagctacc cggctgcccg
      361 gctgcgcgcg gcaggtccag gacccgaacc ccgctcccca gcgcctgagc gcctgcaagc
      421 cgccggcggg atgccgcgcg tcgcctgaga gagccgcgcc ggggcgggag cagggagcgg
      481 gccggcccgg gcgcacccgg gagcgcaggg cgccccacgc cgcagccgca gccgagcgcg
      541 ccggcgcccg cctcccccgc ccggctcgca ggccccggct cctcaagccc cgagggccgc
      601 ggggccgcca gccagccaca cgcctcggcg tcaggggcat ggaggaacgg cgggctccga
      661 gccgcgcccc ggagtccgcg aaacttccga gcgggcgccc gtccgccctg ccgccgccgc
      721 cgccgccgct tcgcctgccg gcctgagagc gggaccatgg atgaaaggtt caacaagtgg
      781 ctgctgacgc cggtgctcac tctcctcttc gtggtcatca tgtaccagta cgtgtccccc
      841 tcctgcacca gctcctgcac caacttcggg gagcagcccc gcgcggggga ggccggcccg
      901 cccgccgtcc cgggtcccgc ccgccgggct caggcgccgc cggaggagtg ggagcggcgg
      961 ccccagttgc ccccgccgcc ccgggggccc cccgagggac ctcggggggc cgcggcgccg
     1021 gaggaggagg acgaggagcc cggagacccc cgggaggggg aggaagagga ggaggaagac
     1081 gagccggacc ccgaggcccc ggaaaacggc tccctgcccc gattcgtgcc gcgcttcaac
     1141 ttcagcctga aggacctgac ccgcttcgtg gatttcaaca tcaaagggcg cgacgtgatc
     1201 gtgttcctcc acatccagaa gacggggggc accactttcg gccggcacct ggtgaagaac
     1261 atccggctgg agcagccttg tagctgcaaa gcgggtcaga agaagtgcac ctgccaccgg
     1321 cctggcaaga aggagacgtg gctcttctcc cgcttctcca ccggctggag ctgcgggctg
     1381 cacgccgact ggacggagct caccaactgc gtgccggcca tcatggagaa gaaggactgt
     1441 ccccgcaacc acagccacac caggaatttc tattacatca caatgttacg ggatccagtg
     1501 tcacgttacc tgagcgagtg gaaacatgtc cagagagggg ccacttggaa aacctctctt
     1561 catatgtgtg atggaagaag ccccacccca gatgagctgc ctacctgcta ccctggggat
     1621 gactggtctg gggtcagctt gcgggagttt atggattgca cctacaacct ggctaacaat
     1681 cgccaggtgc gcatgctggc tgacctcagc ctggtgggct gctataactt gactttcatg
     1741 aacgagagtg aaagaaacac catcctgttg cagagtgcaa agaacaacct gaagaacatg
     1801 gccttctttg ggctcactga gttccagagg aagacacagt ttctctttga gagaacattc
     1861 aacctcaagt tcatctcccc cttcacacag ttcaacatca cgcgggcttc taacgtggag
     1921 atcaacgagg gtgcccgcca acgcattgag gatctaaact tcctggacat gcagctttac
     1981 gagtatgcaa aagatctctt ccagcagcgc taccaccaca ccaagcagct agagcaccag
     2041 agggaccgcc agaagcggcg ggaggagcgg aggctgcagc gagagcacag ggaccaccag
     2101 tggcccaaag aagatggggc tgcagaaggg actgtcaccg aggactacaa cagccaggtg
     2161 gtgagatggt gacctcctgc cctctcctct ctcaggaggg ggagggtgag caggcacatt
     2221 gactttctgt tgaggtacct tggagaagct gagccattct gaggacatct ggctgtgtgt
     2281 gcttgatttg gacatcttct tccttctttg tcttcatttt tatccagctg gagattatcc
     2341 gtcttgttct tttttttctt gacattttgc aattggtgat attaagtagg gtaggagtgc
     2401 atcccatata ggccatttta gaaggccaag gagagccaca ccgaaaaagg agacagttcc
     2461 tgtgatctcc tttgcaggag catagaatag tttgggtacc aggaacccac agaggcacac
     2521 atgaaaagcc aaattatggc ttggatgttc tgctgaaact ggtctatgtc atactgtctc
     2581 ctgttatgag aatatcagtt ggtataaaga gagagaaaga gaaaaacatt tcagccctta
     2641 gatgaggtct tacaccaacc cccacttggc tgttggctgt catcttgaac tctatttgaa
     2701 tgtgacttaa atcacaagta acgtgttttg ttgttgcagt tgttttgaaa caaaattatc
     2761 ctattattga ccattgctag agacccacat cctacaaaat cctgacacca taaccttaag
     2821 ccatgccttt ccttccatct tttagggaac ggggagtgga tccaggacag gggaggttgt
     2881 aacccctgag aaatgaacat tggtaggagc atttaagaga aaacttgctt atttgctaat
     2941 gcctaaaggg gtctcacttt accaattgtt actttcaatg tgaggaatga atgataaatt
     3001 aagagaaaaa aatatcagga aggtcaatat atgcctaaca tttctaaact ggccacataa
     3061 ccttagattt ttaaaggaat atttcagagt ttaatctttt tggagaagtt atgttcttta
     3121 atcgggtact accagtcttg aaattttgcc accacagaaa gaaagatgtt tttaaaagct
     3181 tggcccaaag aataggaact tagctagcat gtatacaaaa tatatttgag gttactaaat
     3241 gaataaaaac tcggaattag gccaggagac atggaatacc attcatggga tggttatcac
     3301 aggagtttag aaagactctg cctcccagca tcttgaatat ctccagcctg ttcactgttt
     3361 tcttcctaca tgatctttac ctgttgttag actgtggcca cagccagtaa atcaggccag
     3421 agtgtctcaa actggacaag aaatgtgctg cattgccgag ttcttatttt caccttttca
     3481 taatcccaac tacaataaca agaattttat tgactaataa gggaatatgt atggaagaag
     3541 gaagagaaag cctttgtcta gcatttatta ggaggatcag agtgagtggg aagtttcaca
     3601 acggattgat ctgatcaatc ccttcaagga gctgaaggca aaatgtgtaa aacccctaaa
     3661 tcagattgaa aagcctattt cattgatatc caacatgttt gatgtttaag ctagctttac
     3721 ctaagccact tctcagcctc cagaatactc tctctggggt tgtgagtcag tcagcccaca
     3781 agcattcaca gagtgcttac ctgccccgag ggaagtccag cagctccacc agatagagat
     3841 ctggagatgc ttctctcact gtgcttggct tcatctgggg tgcaagggaa ggcattatcg
     3901 ggtggctccc ttgctccagg aactgaacga ggcccttttc ataccttaaa tatattctgc
     3961 acaacaaatg ggtattgtta gtctccattt catagatgaa aatagtgaag cccacagaaa
     4021 ataagtagct cctccacagg ttgtaagcta ggtctagctg actgcaaagg tcaagttctc
     4081 tttactccgt caccatatcc atatggaata agggctcgtc ctacctaaag cttcctgcca
     4141 ataatgcaag aagccagctg caatttccca gaagcatttg tctctaaccc atgtgggtag
     4201 cccgaccatg acagcaccac gctaacagtg acccagagcc atactgcctt gggtcagtcc
     4261 agccccctcc acatcactga gccatcaatg agctgagaga tcaatgactt tttaggcagc
     4321 tgtgtttttt aagccatata caagagtaag ttaaaagggg ccaagtaaat gttgcttgtt
     4381 atttggtaga gattaggctc acaaaatatt tctccatttc cagtgatttg ttcctaaggt
     4441 agaagggaag gaggattgca gctgggatta atgttcgtct ctctctctct ctctctctag
     4501 cttctcatct cattgttagg atctaatgtt ctgcctagaa tagcaggact gcaaattatc
     4561 cttctgcctg cccctgtgac acacacacac acacacacac acacacacaa acaatccagt
     4621 gttttgtcat gtggaaaatc aaaacaagtt agaaagcatt cagattgttt cttttaaact
     4681 cactttaaaa ttttggcaag gaattcatgc ataactgaga cttggaggca gcccctgctc
     4741 acttcacgct gttccttagg cacctcggga ttggtatcaa aggcttccac tgccttctgc
     4801 tgaaggcatg atgtatgcgg ctccacttga gaccaggtat caggatgttc agagggagcc
     4861 agctctttat gttggcccca ggccagtact aagcaaatta aaaagaccga catgggcttc
     4921 ctccctcata tcatttcatt ctaaagcagc aagcttgtca tacccatggg gtcctatgtt
     4981 gatagaaatg gagaggtagg gatacagttt attctccaaa aatggactgc ccacttccat
     5041 gctgagtgat gctcagggaa gtgatgcccg cagaaggcgt ctgggcctaa ggatccatgg
     5101 aagtcaggag cattaaatcc caaaccaaat caactccaga tatatcagtg tcaaaagccc
     5161 aagaaaagac aaaaaagaaa aaaacaatcc ccaggggttc tatgacccta ttgactccta
     5221 cagttctttc ccttctcttg gcaatggaag ctccagtacc cagattggag attaggatga
     5281 gacaactttg tgtatatgtg cacgtgtgtg gtgtgtgtgt gttttattaa ggactaagat
     5341 acacacttat ctattctgtc tccttctagc ttttaggcag cctactcttg gaaactggag
     5401 gaaactgccc tgagaattat tagaatgcta ataatagtaa ttggtaacat taacaatcag
     5461 tgttagacaa cagtgaggca tatcatgtgt ttttattaaa aatgataaag atgtgtttct
     5521 tctcatctaa aggaggtaac tttgtgataa ttagtttgat taaaagtttg aagtatgagg
     5581 actccttgtt atgcctttaa ggtaattttt aaaaaaaatc tgagtagaga tagtactaat
     5641 attggcaaga aggtccccag gatagaccta gcaaggagat aagaatagat cattaaaaaa
     5701 taaatataaa tggattggat agaattccca ttcagctgtt ttaacacaga aattcaaaat
     5761 agtgacttaa catggaagct atttctctct tacttaaagg caaactggtt ttacagctct
     5821 ggtgtgtgat gttaccagac gcctaggccc tctcctgttg ctctgtcatc ctaatgtgct
     5881 gcatccacct catggaccaa gatggctgcc acagctcctg ccatcacatt tacatttaag
     5941 atagtagaga ggaagtggag ggaagttgca catattgctt ccagtcacat ctcatgggtt
     6001 aaaacctaga catttgccac atctagctgc aaagaaggct agggaatgtc tttattattg
     6061 attgtcatgt gccttgataa gcataggagg ttctgttatt aaagaaacaa tgtgaaataa
     6121 tgtggaaatt aatactgata aagactatca gtatctgata caaaagggaa ccaaacgata
     6181 tttaagtgct ttttaatgtc acttacttca tcatatgttt tgcgaaagat aagactaaaa
     6241 ttcatcactt tgtgtagcat ttaatggttt tcagaacatt tttgcatgtg ctgtctcatt
     6301 tggtcctcat tacattcttg tgtgctaaat aggacctcta ttcattacac cacttgctag
     6361 tttgggatgg agagtcagat atgttgaaag atttcccaac gactctaatt tatgagcaag
     6421 aaagttgctt agagccccag ccttttgact tcatagtctt tccgactact ttatgcagct
     6481 cagcatcagt actgggattg ggaactgcaa gaatatgaaa tgtaaatctt tattgattta
     6541 tatccttcct tacctttttt gacatggaaa atgccaaaga cactggtcaa gtttactctc
     6601 agctccctac ctccagtgag ggggcctctt attaggaatg aaatatccaa gagatgggcg
     6661 gtgctgcagg ggagaccaaa actcagagga ggcattcaaa cacttctagc aggttgtcta
     6721 ctccatccaa ctcaaaacct attcaatatg aaggccgagg gaatgtgctc acaactccct
     6781 attaagggaa atcaatataa acccgccccc accaaccaaa aaaaaaaaaa gaaagaaaac
     6841 tctccctttt taatgtcttc ttccccaggc tcctggtact ttgtcagcaa catactgctt
     6901 tcattcttaa aggtttcatt ggctgttaaa tgagtccctt tgcatactca ctggagagag
     6961 gaaatgcttt aagttgtcct aagataacat ctgcacacag agagtttatc tgattcctta
     7021 tcaaccatga tctctatctg aggtcctgta tagtgggatc atttagagat gggggttagg
     7081 gagagtagga gtaactctag agaaaattgt ggctcctaaa ggaggcagat ctttccaagg
     7141 tggaaaggta atgaattaaa tatatgacat gtaatactga aaaaatagaa aaatgactaa
     7201 gatacagtct ctgtcctcta gaactagggc agcctggaaa catatggact ataattattt
     7261 tagctattta aaagaaatga atgaagagga tcttgagaca ggaccagagc attggaatgg
     7321 ttgtagggac tgaaagctga gttggaagga tcagatcaaa gagaaagctg ggttggactt
     7381 acacaagaat accctgatct gatcgcagct gtgactccaa ggttttcctt tcccatagtt
     7441 gctgtgaccc cattggtcac cagagcaact gcatgtcttc aggctgggta ttagggctgg
     7501 ctgggtctga tgattctacc cattttgtgc catctgagga gcatgcgagg gttctttctc
     7561 caaggcactt tcggccttcc ttcttctccc cttccgccat ctccactgga cataagccaa
     7621 tgcctgctct cttctcacca cattcacctg gacaaatctg aactaattcc ttgagacttc
     7681 aaattagccc ttctgagcaa gtgggcaggt tctgaaacct gtgtgttttg tagcctaatg
     7741 aattgatgga atgcacatga acccatcttc acagcatgtg aaagaaccaa cctgcctggc
     7801 agaggtgcag aaatttccag aggactggcc tagctataaa caccagcaac atctgcaaca
     7861 caaaagccct tgaacttcta ggcaaggact catgctaacc tagcagtact ccagattgag
     7921 aacgcagatg gacaggtgtc tcttgagcct gtgttccaag atgtctgtgg aaaggattgc
     7981 aactgtcatc ctttggcaca ctcagtggaa cctgtgtgag ctacatgttc tgctgcatta
     8041 tgggaactca tcaaagggcc agattgacat cttgccagaa aaaccagtgt tttgaggagc
     8101 aaaagttttg tgtacttcag taattgtcac atgtgtcttc cacctccttg acatttattt
     8161 gttaatcata tgatcatttt tttttcactt tccttttttt ctttctcaga agtacttctg
     8221 gggctttgat tgcattattt ctcttttaat gtatcatttc agtgttggaa agaaaaatct
     8281 ggatctttat gtggggacct attttacatg tagatagtta ttcagtagat agtaaattat
     8341 ttctcttaag agatatttcc ttttatttct gtacaataat atgtatgaac tcagttacta
     8401 ggggactgta ttgtgacatt atcaaccttt attgctattt aaaaatgata gtttgtagaa
     8461 ctgaagattt ccattgatgt gaagcacaat ttaatgaata agttaaaata ttaaactgtt
     8521 gtgatgtcaa gagcaa
//
