LOCUS       NM_001394073            4173 bp    mRNA    linear   PRI 08-APR-2024
DEFINITION  Homo sapiens heparan sulfate 6-O-sulfotransferase 2 (HS6ST2),
            transcript variant 3, mRNA.
ACCESSION   NM_001394073
VERSION     NM_001394073.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Li,S., Shen,H., Wan,Q., Chen,P., Shu,C., Wang,H. and Zhu,W.
  TITLE     Downregulation of HS6ST2 Inhibits Cervical Cancer Cell Migration
            and Invasion in Vivo and in Vitro
  JOURNAL   Discov Med 35 (179), 1147-1159 (2023)
   PUBMED   38058080
  REMARK    GeneRIF: Downregulation of HS6ST2 Inhibits Cervical Cancer Cell
            Migration and Invasion in Vivo and in Vitro.
REFERENCE   2  (bases 1 to 4173)
  AUTHORS   Zhang,Y., Yu,Y., Cao,X. and Chen,P.
  TITLE     Role of lncRNA FAM83H antisense RNA1 (FAM83H-AS1) in the
            progression of non-small cell lung cancer by regulating the
            miR-545-3p/heparan sulfate 6-O-sulfotransferase (HS6ST2) axis
  JOURNAL   Bioengineered 13 (3), 6476-6489 (2022)
   PUBMED   35260044
  REMARK    GeneRIF: Role of lncRNA FAM83H antisense RNA1 (FAM83H-AS1) in the
            progression of non-small cell lung cancer by regulating the
            miR-545-3p/heparan sulfate 6-O-sulfotransferase (HS6ST2) axis.
REFERENCE   3  (bases 1 to 4173)
  AUTHORS   Paganini,L., Hadi,L.A., Chetta,M., Rovina,D., Fontana,L.,
            Colapietro,P., Bonaparte,E., Pezzani,L., Marchisio,P., Tabano,S.M.,
            Costanza,J., Sirchia,S.M., Riboni,L., Milani,D. and Miozzo,M.
  TITLE     A HS6ST2 gene variant associated with X-linked intellectual
            disability and severe myopia in two male twins
  JOURNAL   Clin Genet 95 (3), 368-374 (2019)
   PUBMED   30471091
  REMARK    GeneRIF: we identified a novel c.916G>C variant in the HS6ST2 gene.
            The mutation caused a significantly reduction in HS6ST2
            6-O-sulfotransferase activity and was associated with a previously
            undescribed form of syndromic XLID with severe congenital myopia
REFERENCE   4  (bases 1 to 4173)
  AUTHORS   Guo,Y., Min,Z., Jiang,C., Wang,W., Yan,J., Xu,P., Xu,K., Xu,J.,
            Sun,M., Zhao,Y., Hussain,S., Zhang,R., Wang,Q., Han,Y., Zhang,F.,
            Zhu,W., Li,D., Meng,L., Sun,J. and Lu,S.
  TITLE     Downregulation of HS6ST2 by miR-23b-3p enhances matrix degradation
            through p38 MAPK pathway in osteoarthritis
  JOURNAL   Cell Death Dis 9 (6), 699 (2018)
   PUBMED   29899528
  REMARK    GeneRIF: downregulated HS6ST2 targeted by miR-23b-3p promotes
            matrix degradation by activating p38 MAPK in chondrocytes and OA
            cartilage.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4173)
  AUTHORS   Ushakov,V.S., Tsidulko,A.Y., de La Bourdonnaye,G., Kazanskaya,G.M.,
            Volkov,A.M., Kiselev,R.S., Kobozev,V.V., Kostromskaya,D.V.,
            Gaytan,A.S., Krivoshapkin,A.L., Aidagulova,S.V. and Grigorieva,E.V.
  TITLE     Heparan Sulfate Biosynthetic System Is Inhibited in Human Glioma
            Due to EXT1/2 and HS6ST1/2 Down-Regulation
  JOURNAL   Int J Mol Sci 18 (11), 2301 (2017)
   PUBMED   29104277
  REMARK    GeneRIF: RT-PCR analysis showed that the overall transcriptional
            activity of the main Heparan Sulfate biosynthesis-involved genes
            (EXT1, EXT2, NDST1, NDST2, GLCE, HS2ST1, HS3ST1, HS3ST2, HS6ST1,
            HS6ST2, SULF1, SULF2, HPSE) was decreased by 1.5-2-fold in Grade
            II-III glioma.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4173)
  AUTHORS   Teumer,A., Holtfreter,B., Volker,U., Petersmann,A., Nauck,M.,
            Biffar,R., Volzke,H., Kroemer,H.K., Meisel,P., Homuth,G. and
            Kocher,T.
  TITLE     Genome-wide association study of chronic periodontitis in a general
            German population
  JOURNAL   J Clin Periodontol 40 (11), 977-985 (2013)
   PUBMED   24024966
REFERENCE   7  (bases 1 to 4173)
  AUTHORS   Song,K., Li,Q., Peng,Y.B., Li,J., Ding,K., Chen,L.J., Shao,C.H.,
            Zhang,L.J. and Li,P.
  TITLE     Silencing of hHS6ST2 inhibits progression of pancreatic cancer
            through inhibition of Notch signalling
  JOURNAL   Biochem J 436 (2), 271-282 (2011)
   PUBMED   21443520
  REMARK    GeneRIF: HS6ST2 knockdown disrupts epithelial-mesenchymal
            transition and suggesets that HS6ST2 potentiates Notch signaling in
            pancreatic cancer cells.
REFERENCE   8  (bases 1 to 4173)
  AUTHORS   Ross,M.T., Grafham,D.V., Coffey,A.J., Scherer,S., McLay,K.,
            Muzny,D., Platzer,M., Howell,G.R., Burrows,C., Bird,C.P.,
            Frankish,A., Lovell,F.L., Howe,K.L., Ashurst,J.L., Fulton,R.S.,
            Sudbrak,R., Wen,G., Jones,M.C., Hurles,M.E., Andrews,T.D.,
            Scott,C.E., Searle,S., Ramser,J., Whittaker,A., Deadman,R.,
            Carter,N.P., Hunt,S.E., Chen,R., Cree,A., Gunaratne,P., Havlak,P.,
            Hodgson,A., Metzker,M.L., Richards,S., Scott,G., Steffen,D.,
            Sodergren,E., Wheeler,D.A., Worley,K.C., Ainscough,R.,
            Ambrose,K.D., Ansari-Lari,M.A., Aradhya,S., Ashwell,R.I.,
            Babbage,A.K., Bagguley,C.L., Ballabio,A., Banerjee,R., Barker,G.E.,
            Barlow,K.F., Barrett,I.P., Bates,K.N., Beare,D.M., Beasley,H.,
            Beasley,O., Beck,A., Bethel,G., Blechschmidt,K., Brady,N.,
            Bray-Allen,S., Bridgeman,A.M., Brown,A.J., Brown,M.J., Bonnin,D.,
            Bruford,E.A., Buhay,C., Burch,P., Burford,D., Burgess,J.,
            Burrill,W., Burton,J., Bye,J.M., Carder,C., Carrel,L., Chako,J.,
            Chapman,J.C., Chavez,D., Chen,E., Chen,G., Chen,Y., Chen,Z.,
            Chinault,C., Ciccodicola,A., Clark,S.Y., Clarke,G., Clee,C.M.,
            Clegg,S., Clerc-Blankenburg,K., Clifford,K., Cobley,V., Cole,C.G.,
            Conquer,J.S., Corby,N., Connor,R.E., David,R., Davies,J., Davis,C.,
            Davis,J., Delgado,O., Deshazo,D., Dhami,P., Ding,Y., Dinh,H.,
            Dodsworth,S., Draper,H., Dugan-Rocha,S., Dunham,A., Dunn,M.,
            Durbin,K.J., Dutta,I., Eades,T., Ellwood,M., Emery-Cohen,A.,
            Errington,H., Evans,K.L., Faulkner,L., Francis,F., Frankland,J.,
            Fraser,A.E., Galgoczy,P., Gilbert,J., Gill,R., Glockner,G.,
            Gregory,S.G., Gribble,S., Griffiths,C., Grocock,R., Gu,Y.,
            Gwilliam,R., Hamilton,C., Hart,E.A., Hawes,A., Heath,P.D.,
            Heitmann,K., Hennig,S., Hernandez,J., Hinzmann,B., Ho,S., Hoffs,M.,
            Howden,P.J., Huckle,E.J., Hume,J., Hunt,P.J., Hunt,A.R.,
            Isherwood,J., Jacob,L., Johnson,D., Jones,S., de Jong,P.J.,
            Joseph,S.S., Keenan,S., Kelly,S., Kershaw,J.K., Khan,Z.,
            Kioschis,P., Klages,S., Knights,A.J., Kosiura,A., Kovar-Smith,C.,
            Laird,G.K., Langford,C., Lawlor,S., Leversha,M., Lewis,L., Liu,W.,
            Lloyd,C., Lloyd,D.M., Loulseged,H., Loveland,J.E., Lovell,J.D.,
            Lozado,R., Lu,J., Lyne,R., Ma,J., Maheshwari,M., Matthews,L.H.,
            McDowall,J., McLaren,S., McMurray,A., Meidl,P., Meitinger,T.,
            Milne,S., Miner,G., Mistry,S.L., Morgan,M., Morris,S., Muller,I.,
            Mullikin,J.C., Nguyen,N., Nordsiek,G., Nyakatura,G., O'Dell,C.N.,
            Okwuonu,G., Palmer,S., Pandian,R., Parker,D., Parrish,J.,
            Pasternak,S., Patel,D., Pearce,A.V., Pearson,D.M., Pelan,S.E.,
            Perez,L., Porter,K.M., Ramsey,Y., Reichwald,K., Rhodes,S.,
            Ridler,K.A., Schlessinger,D., Schueler,M.G., Sehra,H.K.,
            Shaw-Smith,C., Shen,H., Sheridan,E.M., Shownkeen,R., Skuce,C.D.,
            Smith,M.L., Sotheran,E.C., Steingruber,H.E., Steward,C.A.,
            Storey,R., Swann,R.M., Swarbreck,D., Tabor,P.E., Taudien,S.,
            Taylor,T., Teague,B., Thomas,K., Thorpe,A., Timms,K., Tracey,A.,
            Trevanion,S., Tromans,A.C., d'Urso,M., Verduzco,D., Villasana,D.,
            Waldron,L., Wall,M., Wang,Q., Warren,J., Warry,G.L., Wei,X.,
            West,A., Whitehead,S.L., Whiteley,M.N., Wilkinson,J.E.,
            Willey,D.L., Williams,G., Williams,L., Williamson,A.,
            Williamson,H., Wilming,L., Woodmansey,R.L., Wray,P.W., Yen,J.,
            Zhang,J., Zhou,J., Zoghbi,H., Zorilla,S., Buck,D., Reinhardt,R.,
            Poustka,A., Rosenthal,A., Lehrach,H., Meindl,A., Minx,P.J.,
            Hillier,L.W., Willard,H.F., Wilson,R.K., Waterston,R.H., Rice,C.M.,
            Vaudin,M., Coulson,A., Nelson,D.L., Weinstock,G., Sulston,J.E.,
            Durbin,R., Hubbard,T., Gibbs,R.A., Beck,S., Rogers,J. and
            Bentley,D.R.
  TITLE     The DNA sequence of the human X chromosome
  JOURNAL   Nature 434 (7031), 325-337 (2005)
   PUBMED   15772651
REFERENCE   9  (bases 1 to 4173)
  AUTHORS   Habuchi,H., Miyake,G., Nogami,K., Kuroiwa,A., Matsuda,Y.,
            Kusche-Gullberg,M., Habuchi,O., Tanaka,M. and Kimata,K.
  TITLE     Biosynthesis of heparan sulphate with diverse structures and
            functions: two alternatively spliced forms of human heparan
            sulphate 6-O-sulphotransferase-2 having different expression
            patterns and properties
  JOURNAL   Biochem J 371 (Pt 1), 131-142 (2003)
   PUBMED   12492399
  REMARK    GeneRIF: we have characterized HS6ST-2 and HS6ST-1 human isologues,
            including their chromosomal localizations,HS6STs could also
            transfer sulfate to N -sulfoglucosamine residues located at the
            non-reducing terminal of HS with high affinity.
REFERENCE   10 (bases 1 to 4173)
  AUTHORS   Habuchi,H., Tanaka,M., Habuchi,O., Yoshida,K., Suzuki,H., Ban,K.
            and Kimata,K.
  TITLE     The occurrence of three isoforms of heparan sulfate
            6-O-sulfotransferase having different specificities for hexuronic
            acid adjacent to the targeted N-sulfoglucosamine
  JOURNAL   J Biol Chem 275 (4), 2859-2868 (2000)
   PUBMED   10644753
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from Z81365.2 and Z86064.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            Summary: Heparan sulfate proteoglycans are ubiquitous components of
            the cell surface, extracellular matrix, and basement membranes, and
            interact with various ligands to influence cell growth,
            differentiation, adhesion, and migration. This gene encodes a
            member of the heparan sulfate (HS) sulfotransferase gene family,
            which catalyze the transfer of sulfate to HS. Different family
            members and isoforms are thought to synthesize heparan sulfates
            with tissue-specific structures and functions. Multiple transcript
            variants encoding different isoforms have been found for this gene.
            [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038193.4396219.1,
                                           SRR14038194.1888256.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2145743, SAMEA2159080
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000370833.7/ ENSP00000359870.3
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-455               Z81365.2           4774-5228           c
            456-974             Z81365.2           3407-3925           c
            975-1007            Z86064.2           113784-113816       c
            1008-1094           Z86064.2           74435-74521         c
            1095-4173           Z86064.2           31337-34415         c
FEATURES             Location/Qualifiers
     source          1..4173
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq26.2"
     gene            1..4173
                     /gene="HS6ST2"
                     /gene_synonym="MRXSPM"
                     /note="heparan sulfate 6-O-sulfotransferase 2"
                     /db_xref="GeneID:90161"
                     /db_xref="HGNC:HGNC:19133"
                     /db_xref="MIM:300545"
     exon            1..455
                     /gene="HS6ST2"
                     /gene_synonym="MRXSPM"
                     /inference="alignment:Splign:2.1.0"
     CDS             28..1965
                     /gene="HS6ST2"
                     /gene_synonym="MRXSPM"
                     /EC_number="2.8.2.-"
                     /note="isoform L is encoded by transcript variant 3;
                     HS6ST-2"
                     /codon_start=1
                     /product="heparan-sulfate 6-O-sulfotransferase 2 isoform
                     L"
                     /protein_id="NP_001381002.1"
                     /db_xref="CCDS:CCDS48170.1"
                     /db_xref="GeneID:90161"
                     /db_xref="HGNC:HGNC:19133"
                     /db_xref="MIM:300545"
                     /translation="MALPACAVREFEPPRQPERGAPVRTTCPRRHSRVEAELAASRPG
                     SVAASVRAGPPRGVSHGFHTRPLLDKPRKASSSLAGAACAPLFALLSRGRRRRMHVLR
                     RRWDLGSLCRALLTRGLAALGHSLKHVLGAIFSKIFGPMASVGNMDEKSNKLLLALVM
                     LFLFAVIVLQYVCPGTECQLLRLQAFSSPVPDPYRSEDESSARFVPRYNFTRGDLLRK
                     VDFDIKGDDLIVFLHIQKTGGTTFGRHLVRNIQLEQPCECRVGQKKCTCHRPGKRETW
                     LFSRFSTGWSCGLHADWTELTSCVPSVVDGKRDARLRPSRWRIFQILDAASKDKRGSP
                     NTNAGANSPSSTKTRNTSKSGKNFHYITILRDPVSRYLSEWRHVQRGATWKASLHVCD
                     GRPPTSEELPSCYTGDDWSGCPLKEFMDCPYNLANNRQVRMLSDLTLVGCYNLSVMPE
                     KQRNKVLLESAKSNLKHMAFFGLTEFQRKTQYLFEKTFNMNFISPFTQYNTTRASSVE
                     INEEIQKRIEGLNFLDMELYSYAKDLFLQRYQFMRQKEHQEARRKRQEQRKFLKGRLL
                     QTHFQSQGQGQSQNPNQNQSQNPNPNANQNLTQNLMQNLTQSLSQKENRESPKQNSGK
                     EQNDNTSNGTNDYIGSVEKWR"
     exon            456..974
                     /gene="HS6ST2"
                     /gene_synonym="MRXSPM"
                     /inference="alignment:Splign:2.1.0"
     exon            975..1007
                     /gene="HS6ST2"
                     /gene_synonym="MRXSPM"
                     /inference="alignment:Splign:2.1.0"
     exon            1008..1094
                     /gene="HS6ST2"
                     /gene_synonym="MRXSPM"
                     /inference="alignment:Splign:2.1.0"
     exon            1095..4173
                     /gene="HS6ST2"
                     /gene_synonym="MRXSPM"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4156..4161
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HS6ST2"
                     /gene_synonym="MRXSPM"
                     /note="hexamer: AATAAA"
     polyA_site      4173
                     /gene="HS6ST2"
                     /gene_synonym="MRXSPM"
                     /note="major polyA site"
ORIGIN      
        1 agtaccctga gttgcctgaa ggggggaatg gcactgcctg cgtgtgcagt ccgggagttc
       61 gagccgccgc ggcaaccgga gcgaggagcg cccgtccgca ccacctgtcc ccgccggcat
      121 tccagagtag aggccgaatt ggcagcgagc cggcccgggt cggtcgccgc ctcagttcgc
      181 gcgggccctc ctaggggtgt gtctcacgga ttccacaccc ggccgctcct ggacaagccc
      241 cgaaaggcgt cttcttccct ggcgggagcc gcgtgcgccc cgcttttcgc gctgctgtcc
      301 cggggccgcc gcaggcggat gcacgtcctc aggcgacgct gggacctggg ctccctctgc
      361 cgggccctgc tcactcgggg cctggccgcc ctgggccact cgctgaagca cgtgctcggt
      421 gcgatcttct ccaagatttt cggccccatg gccagcgtcg ggaacatgga tgagaaatcc
      481 aacaagctgc tgctagcttt ggtgatgctc ttcctatttg ccgtgatcgt cctccaatac
      541 gtgtgccccg gcacagaatg ccagctcctc cgcctgcagg cgttcagctc cccggtgccg
      601 gacccgtacc gctcggagga tgagagctcc gccaggttcg tgccccgcta caatttcacc
      661 cgcggcgacc tcctgcgcaa ggtagacttc gacatcaagg gcgatgacct gatcgtgttc
      721 ctgcacatcc agaagaccgg gggcaccact ttcggccgcc acttggtgcg taacatccag
      781 ctggagcagc cgtgcgagtg ccgcgtgggt cagaagaaat gcacttgcca ccggccgggt
      841 aagcgggaaa cctggctctt ctccaggttc tccacgggct ggagctgcgg gttgcacgcc
      901 gactggaccg agctcaccag ctgtgtgccc tccgtggtgg acggcaagcg cgacgccagg
      961 ctgagaccgt ccaggtggag gatttttcag attctagatg cagcaagtaa ggataaacgg
     1021 ggttctccaa acactaacgc aggcgccaac tctccgtcat ccacaaagac ccggaacaca
     1081 tctaagagtg ggaagaactt ccactacatc accatcctcc gagacccagt gtcccggtac
     1141 ttgagtgagt ggaggcatgt ccagagaggg gcaacatgga aagcatccct gcatgtctgc
     1201 gatggaaggc ctccaacctc cgaagagctg cccagctgct acactggcga tgactggtct
     1261 ggctgccccc tcaaagagtt tatggactgt ccctacaatc tagccaacaa ccgccaggtg
     1321 cgcatgctct ccgacctgac cctggtaggc tgctacaacc tctctgtcat gcctgaaaag
     1381 caaagaaaca aggtccttct ggaaagtgcc aagtcaaatc tgaagcacat ggcgttcttc
     1441 ggcctcactg agtttcagcg gaagacccaa tatctgtttg agaaaacctt caacatgaac
     1501 tttatttcgc catttaccca gtataatacc actagggcct ctagtgtaga gatcaatgag
     1561 gaaattcaaa agcgtattga gggactgaat tttctggata tggagttgta cagctatgcc
     1621 aaagaccttt ttttgcagag gtatcagttt atgaggcaga aagagcatca ggaggccagg
     1681 cgaaagcgtc aggaacaacg caaatttctg aagggaaggc tccttcagac ccatttccag
     1741 agccagggtc agggccagag ccagaatccg aatcagaatc agagtcagaa cccaaatccg
     1801 aatgccaatc agaacctgac tcagaatctg atgcagaatc tgactcagag tttgagccag
     1861 aaggagaacc gggaaagccc gaagcagaac tcaggcaagg agcagaatga taacaccagc
     1921 aatggcacca acgactacat aggcagtgta gagaaatggc gttaaatggc tcaaaaaggc
     1981 ctgtacatac ttctcccaaa gcgccactga aaagatggca tagcttaaaa gatgaaagtg
     2041 tccaaacaca tcctgcttcc ttcattgggg aagttttaaa aaaaagttta gatgttgcct
     2101 ttacagttgc ctttcaattc agtgttatac tgtgtgtagg taaaacaaat ctcaatatgg
     2161 aattaaattg tctttttggg gttggactaa atatgaaatc cgaaagccaa accagactca
     2221 ccagaaattg ctgtttagat attttaagaa gttcttaaat tagttatgga gacaaagtga
     2281 aaacataaaa tgtgaccatt taacttatgg ctaagaaatg gactttaaat tattcatgat
     2341 acactgttaa aacccaatct tggaatcaaa tattttttcc aggggtgaga ataagtataa
     2401 acataaagca actaaaatga aacataaaac cttttatttt cttctgattt taacaaggaa
     2461 tctatttaaa tagaataaca actgatggtg aatcttaccg agctgtagaa aataaaaaat
     2521 tcctctccaa acatgggtag ttttatgtca aaatattggc ttttcaagaa caggactcat
     2581 atcttgatat ttaagagatg tttaaaattt taaacttttt ctaccttcta ctgtttaaag
     2641 gttttacaca gggtgtatct cacattaaac aaaacacctt tttttcaaaa tgaaatacca
     2701 atgtaaagat ctaatttcca ggcgctttca gggcactgta atttcaacaa tactggaatc
     2761 attttggcgc tgcttctcat tcattttaag gcttctctga attgtgctca ttccaaatta
     2821 acccatgtat agaatctttc ttcatcatct aaatggtgtg ttgctgaagt tattgtggta
     2881 tataatcctg gattaaagtc aggactttct ttctatagaa ttattttatc aatatttgtg
     2941 tagtgaggtc ctaatcaaga aacttgaaca ggataatgtg tctaaaaata ttgctgaagt
     3001 tattcttgat cagatagaat ttaacctaaa atgtgaactc ttacttactt tttgtacatc
     3061 tctattgtaa tgtttccagt tgggccaact atttctagcc ctttagttct ttttcatgtt
     3121 gacagatttg tagacaatta caaaagccaa aaaaggataa aataagcatt aggaaggcta
     3181 gccagttatt caatagcacc ttgataatgt gggcaaaccc ttctacaaag tttttgtttt
     3241 gaaaagagca aaagtaaaaa gtgttctgta gtgaggttac atatcaggaa catatgagtt
     3301 gagcagagta atttggtagt gtaagtcgat gcaaaaatac ttctatggta cctattacaa
     3361 gctgaccaag ataaaaagaa gtatgtaacc cactgtgctg tcaagcatca tgattccggg
     3421 cagttttcat tttactgtgg attttgctat acttatgcaa ggacttacac taacctcctg
     3481 gctgtaaatc aaacaaggtg actgaaagtg gcatttatgt tttcacaagg gacatgctta
     3541 gaagtgtgac cataaagtca catcttgtac aatctgctca tgtgtttcat ctgtagtaat
     3601 atagccacaa actggacaag aagctgggta tttgggttta aaatagattt tcttgactct
     3661 gagcttgtat cagtatttct agcttttgtt ttgtctgaca gtattgcagt cttgaaaatc
     3721 ctgagagcta aaaaactgaa aggaattttt ccacaccttg aagtcacagt gttttagagt
     3781 aaaactattt tgtgtacaat tgtgttttta aaatagttag tagatcacta tggtatttca
     3841 attttcttta gttttaattg aaaatgtttg cttttaaaac tgataggtat tgttggaaag
     3901 caggatgaag cctgagccag tggaaaagct tgttacagaa aaaacatttt gtgttattgc
     3961 tgtggtgtgc atgatttgca aagattaagt gcattttctc tgtctatact gattattgta
     4021 tatagaggat gttataaata tacatataca tttttgccat tatgtaaatc ccatgatttc
     4081 aactgtaaac atctgtccat tggtgtagct ttacaaacca ttcactgatt ttgtgtaatt
     4141 taacaataga tatgaaataa agtttaaatt aca
//
