LOCUS       NM_153612               3869 bp    mRNA    linear   PRI 07-FEB-2025
DEFINITION  Homo sapiens heparan sulfate-glucosamine 3-sulfotransferase 5
            (HS3ST5), mRNA.
ACCESSION   NM_153612 XM_001723146 XM_001725130 XM_001726359 XM_006715379
VERSION     NM_153612.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Wang,Q., Xiang,B., Deng,W., Wu,J., Li,M., Ma,X., Wang,Y., Jiang,L.,
            McAlonan,G., Chua,S.E., Sham,P.C., Hu,X. and Li,T.
  TITLE     Genome-wide association analysis with gray matter volume as a
            quantitative phenotype in first-episode treatment-naive patients
            with schizophrenia
  JOURNAL   PLoS One 8 (9), e75083 (2013)
   PUBMED   24086445
  REMARK    Erratum:[PLoS One. 2015 Apr 07;10(4):e0122945. doi:
            10.1371/journal.pone.0122945.. PMID: 25848962]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3869)
  AUTHORS   Vieira,A.R., McHenry,T.G., Daack-Hirsch,S., Murray,J.C. and
            Marazita,M.L.
  TITLE     Candidate gene/loci studies in cleft lip/palate and dental
            anomalies finds novel susceptibility genes for clefts
  JOURNAL   Genet Med 10 (9), 668-674 (2008)
   PUBMED   18978678
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   3  (bases 1 to 3869)
  AUTHORS   Chen,J. and Liu,J.
  TITLE     Characterization of the structure of antithrombin-binding heparan
            sulfate generated by heparan sulfate 3-O-sulfotransferase 5
  JOURNAL   Biochim Biophys Acta 1725 (2), 190-200 (2005)
   PUBMED   16099108
  REMARK    GeneRIF: Structural analysis of 3-OST-5 HS(act) revealed that the
            antithrombin-binding site of 3-OST-5 HS(act) is located within a
            domain clustered with N-sulfated glucosamine units.
REFERENCE   4  (bases 1 to 3869)
  AUTHORS   Duncan,M.B., Chen,J., Krise,J.P. and Liu,J.
  TITLE     The biosynthesis of anticoagulant heparan sulfate by the heparan
            sulfate 3-O-sulfotransferase isoform 5
  JOURNAL   Biochim Biophys Acta 1671 (1-3), 34-43 (2004)
   PUBMED   15026143
  REMARK    GeneRIF: Results demonstrate that the human 3-O-sulfotransferase
            isoform 5 gene is capable of synthesizing anticoagulant heparan
            sulfate (HS) in CHO cells and may contribute to the biosynthesis of
            HS in humans.
REFERENCE   5  (bases 1 to 3869)
  AUTHORS   Mochizuki,H., Yoshida,K., Gotoh,M., Sugioka,S., Kikuchi,N.,
            Kwon,Y.D., Tawada,A., Maeyama,K., Inaba,N., Hiruma,T., Kimata,K.
            and Narimatsu,H.
  TITLE     Characterization of a heparan sulfate 3-O-sulfotransferase-5, an
            enzyme synthesizing a tetrasulfated disaccharide
  JOURNAL   J Biol Chem 278 (29), 26780-26787 (2003)
   PUBMED   12740361
  REMARK    GeneRIF: Recombinant 3-OST-5 only exhibited sulfotransferase
            activity toward heparan sulfate and heparin. 3-OST-5 was highly
            expressed in fetal brain, followed by adult brain and spinal cord,
            and at very low or undetectable levels in other tissues.
REFERENCE   6  (bases 1 to 3869)
  AUTHORS   Xia,G., Chen,J., Tiwari,V., Ju,W., Li,J.P., Malmstrom,A., Shukla,D.
            and Liu,J.
  TITLE     Heparan sulfate 3-O-sulfotransferase isoform 5 generates both an
            antithrombin-binding site and an entry receptor for herpes simplex
            virus, type 1
  JOURNAL   J Biol Chem 277 (40), 37912-37919 (2002)
   PUBMED   12138164
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL136446.14, AL500524.10 and AL355498.10.
            
            On or before Sep 21, 2020 this sequence version replaced
            XM_006715379.2, NM_153612.3.
            
            Summary: HS3ST5 belongs to a group of heparan sulfate
            3-O-sulfotransferases (EC 2.8.2.23) that transfer sulfate from
            3-prime-phosphoadenosine 5-prime phosphosulfate (PAPS) to heparan
            sulfate and heparin (Mochizuki et al., 2003 [PubMed
            12740361]).[supplied by OMIM, Mar 2008].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853564.23839.1,
                                           SRR14038196.1158985.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2154665, SAMN03267774
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000312719.10/ ENSP00000427888.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-829               AL136446.14        106053-106881       c
            830-1023            AL500524.10        32888-33081         c
            1024-1135           AL355498.10        138103-138214       c
            1136-1274           AL355498.10        32491-32629         c
            1275-3869           AL355498.10        25348-27942         c
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q21-q22.1"
     gene            1..3869
                     /gene="HS3ST5"
                     /gene_synonym="3-OST-5; 3OST5; HS3OST5; NBLA04021"
                     /note="heparan sulfate-glucosamine 3-sulfotransferase 5"
                     /db_xref="GeneID:222537"
                     /db_xref="HGNC:HGNC:19419"
                     /db_xref="MIM:609407"
     exon            1..829
                     /gene="HS3ST5"
                     /gene_synonym="3-OST-5; 3OST5; HS3OST5; NBLA04021"
                     /inference="alignment:Splign:2.1.0"
     exon            830..1023
                     /gene="HS3ST5"
                     /gene_synonym="3-OST-5; 3OST5; HS3OST5; NBLA04021"
                     /inference="alignment:Splign:2.1.0"
     exon            1024..1135
                     /gene="HS3ST5"
                     /gene_synonym="3-OST-5; 3OST5; HS3OST5; NBLA04021"
                     /inference="alignment:Splign:2.1.0"
     exon            1136..1274
                     /gene="HS3ST5"
                     /gene_synonym="3-OST-5; 3OST5; HS3OST5; NBLA04021"
                     /inference="alignment:Splign:2.1.0"
     CDS             1168..2208
                     /gene="HS3ST5"
                     /gene_synonym="3-OST-5; 3OST5; HS3OST5; NBLA04021"
                     /EC_number="2.8.2.23"
                     /note="heparan sulfate 3-OST-5; h3-OST-5; heparan sulfate
                     3-O-sulfotransferase 5; heparan sulfate D-glucosaminyl
                     3-O-sulfotransferase 5; heparan sulfate (glucosamine)
                     3-O-sulfotransferase 5"
                     /codon_start=1
                     /product="heparan sulfate glucosamine 3-O-sulfotransferase
                     5"
                     /protein_id="NP_705840.2"
                     /db_xref="CCDS:CCDS34517.1"
                     /db_xref="GeneID:222537"
                     /db_xref="HGNC:HGNC:19419"
                     /db_xref="MIM:609407"
                     /translation="MLFKQQAWLRQKLLVLGSLAVGSLLYLVARVGSLDRLQPICPIE
                     GRLGGARTQAEFPLRALQFKRGLLHEFRKGNASKEQVRLHDLVQQLPKAIIIGVRKGG
                     TRALLEMLNLHPAVVKASQEIHFFDNDENYGKGIEWYRKKMPFSYPQQITIEKSPAYF
                     ITEEVPERIYKMNSSIKLLIIVREPTTRAISDYTQVLEGKERKNKTYYKFEKLAIDPN
                     TCEVNTKYKAVRTSIYTKHLERWLKYFPIEQFHVVDGDRLITEPLPELQLVEKFLNLP
                     PRISQYNLYFNATRGFYCLRFNIIFNKCLAGSKGRIHPEVDPSVITKLRKFFHPFNQK
                     FYQITGRTLNWP"
     exon            1275..3869
                     /gene="HS3ST5"
                     /gene_synonym="3-OST-5; 3OST5; HS3OST5; NBLA04021"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2990..2995
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HS3ST5"
                     /gene_synonym="3-OST-5; 3OST5; HS3OST5; NBLA04021"
                     /note="hexamer: ATTAAA"
     polyA_site      3011
                     /gene="HS3ST5"
                     /gene_synonym="3-OST-5; 3OST5; HS3OST5; NBLA04021"
                     /note="major polyA site"
     regulatory      3848..3853
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HS3ST5"
                     /gene_synonym="3-OST-5; 3OST5; HS3OST5; NBLA04021"
                     /note="hexamer: AATAAA"
     polyA_site      3869
                     /gene="HS3ST5"
                     /gene_synonym="3-OST-5; 3OST5; HS3OST5; NBLA04021"
                     /note="major polyA site"
ORIGIN      
        1 actctgcagc tggtcgccag ggacccggtc cctcctgcgc gcccaagtga tggaggggaa
       61 gagattcgct tagacacaca cacgcgcaca cagctgggca cgtctccggg aggcagctgc
      121 cggctcgggc gctctggggg ccgggcggcg gccgtgacag cgccagccgc ggggagcggg
      181 gcgcccgcac agcttggcac cggaggctcg aggacgcagg aagggaagat cccagtcaca
      241 ctgagtgttg ctcttttggg cggattggga gggagcgggc tgcccgagga gctgtcaccg
      301 aggacgcccg aagtctcgcc ctctgcgcgc agggtccggg gctgcgccgg tgccctcgtg
      361 gggcaaggag gatgcgcacc tccccccgca gagcggctgc ggtctccggc actgcgcgga
      421 ctccggctcc tgcggccgcc cgtgccgtgc ccgcgccacg ggagcgcgcc ctgccgcacc
      481 tggttccagc ctctcgggga agtgcaggaa acgcgcccta aactccacta cttctcgccc
      541 gcctgccccc ccgcccggct ccccgcctca gacctcgccg ccgccgccgc cgccgccgcc
      601 gcctgcgtgt ggggcccgct cgcgtcttac tcacacgcgc cacgcacagc cctgttggtt
      661 tgctctcatt cacgccgact cgctctccgc cgagcctagc ccgggcatcc gaaactactg
      721 gaccaactgc cgtccggacc gcagcagcgg ccgggtgacc cccacgcgac ccgccgccga
      781 cccccgcgcc ctgaggctcc cgcccgcgcc gccaccgccg gggaccacga gataatcaga
      841 tactcactgt cttcagcatt ggtgtcttct attctcaacg gcatttctgg tggccacccc
      901 aggagtctcc ctgacctgtt ttaaaaccct tggaaattaa ggagagaatc cactacatat
      961 attcctttga aagtttccac catatatgtt caaaaattgc tgaatgagca caaggatttg
     1021 aaggtgctaa agcaagggat aaaccaaaag gacagccagt aggtgaggag aggccatgga
     1081 atacccgcca acaagataat catcgctgga ataataggga cctgtctgaa tgtaggctgc
     1141 agcagtcctg aaggttgatg gagggccatg ctattcaaac agcaggcgtg gctgagacag
     1201 aagctcctgg tgctgggaag ccttgccgtt gggagtctcc tgtatctagt cgccagagtt
     1261 gggagcttgg ataggctaca acccatttgc cccattgaag gtcgactggg tggagcccgc
     1321 actcaggctg aattcccact tcgcgccctg cagtttaagc gtggcctgct gcacgagttc
     1381 cggaagggca acgcttccaa ggagcaggtt cgcctccatg acctggtcca gcagctcccc
     1441 aaggccatta tcattggggt gaggaaagga ggcacaaggg ccctgcttga aatgctgaac
     1501 ctacatccgg cagtagtcaa agcctctcaa gaaatccact tttttgataa tgatgagaat
     1561 tatggtaagg gcattgagtg gtataggaaa aagatgcctt tttcctaccc tcagcaaatc
     1621 acaattgaaa agagcccagc atattttatc acagaggagg ttccagaaag gatttacaaa
     1681 atgaactcat ccatcaagtt gttgatcatt gtcagggagc caaccacaag agctatttct
     1741 gattatactc aggtgctaga ggggaaggag aggaagaaca aaacttatta caagtttgag
     1801 aagctggcca tagaccctaa tacatgcgaa gtgaacacaa aatacaaagc agtaagaacc
     1861 agcatctaca ccaaacatct ggaaaggtgg ttgaaatact ttccaattga gcaatttcat
     1921 gtcgtcgatg gagatcgcct catcacggaa cctctgccag aacttcagct cgtggagaag
     1981 ttcctaaatc tgcctccaag gataagtcaa tacaatttat acttcaatgc taccagaggg
     2041 ttttactgct tgcggtttaa tattatcttt aataagtgcc tggcgggcag caaggggcgc
     2101 attcatccag aggtggaccc ctctgtcatt actaaattgc gcaaattctt tcatcctttt
     2161 aatcaaaaat tttaccagat cactgggagg acattgaact ggccctaaaa taatatgtca
     2221 tacaacacta tgtgttgtgc ctggagacac acaatgtctc ctgtagatta aaatatgcac
     2281 ttttcctagg cagagctatc caagtcattt ttccatgtat atttgtacat acgcagtgtg
     2341 tgaccaaata taagatcagt tctttttcta ctgaaaattt acgaaaaaaa aaaaattgct
     2401 gtctgcatag tcgcatcttt taagctattt acaaaagaga agaggtggtg gtattggggg
     2461 aaagtgactt cagctattct caaagagtta gtcttccttt gattcagaat ttgtcacccg
     2521 ccattttcat agatttaagc caaaagataa atgtgtgaaa atgtaccaat ggctgcgaag
     2581 cttcaggaag tagaggatcc agtgatgcat tttttttttc ctaagggaaa gctggctctt
     2641 taattcagat gctgaattgg tgccatgaaa acagaaaatg ctattttctt attatttaaa
     2701 agaacgtctt atctcataaa attgacattg ttccaaagtt cttgtggtga ttttgcacta
     2761 ttgttttctc gtatggacca tggtgtcact tgtagcatgt caatcacaca ttggaaagtc
     2821 aagtcctttt acttccatgt tgtatgtcaa cagagagaaa tgtcatgtac ataatgtata
     2881 ttgttgtaaa tactggtttc acactaagta attctatttt gtaaactgaa tatggctatt
     2941 taatttattg tgaaaattaa atttattgtg gtatttaaaa atggaatgga ttaaaattac
     3001 tctatgtgca attttttttt tttttactca ttttgtttta cgtgccccct gctggcttcc
     3061 aaaatggaag ctgtttacgt gcatatgaga gcacttggaa agatgtgctt ccctgctgga
     3121 tttctgtacc ccagtgaaaa tgtatttatg aagtgaggtt gagtatatta aaaaagaaaa
     3181 acctcaacca tctggaaatc aagtataata gccacctcaa agaaccctag tgctgctctg
     3241 ctacaacttt gtaacaatta atttactcgc agttgctgct gctcaggaag agagacaagg
     3301 aatattttaa cagaatcaag gcatagaaga atcaccattt tatttgaacc tctaatcaga
     3361 gtcagaccag tagagaaatt aaataagatt agaaaactct gtactgaaag ctgctgatgc
     3421 ttcaaaaatg aaaacaagat ctcacaactc tccctgttag ttgaaaatat atcaatttgc
     3481 tctgaaagga ttcagctgcc tagtgttgcc attactaaca taaacatatg gctcatattt
     3541 ccatccagag aaattaatgc taaattggtg cctcgctaac atcagataca ctgtattatg
     3601 cttaaatata ttcagtaaaa tgtggaaagg ggtattaaca acgacaacaa aaagatggat
     3661 tttttttttc tcacaatcac agttgctaat ccagtgggag atgtttgaga gagttttgtt
     3721 caacatcaca gtgagagtgc ctagggaaat cagaaaatta caatggattc ccctttgatt
     3781 gtaataagtg ttgattttct ccatgagttg tttatcctgt ctagtgattt gatggtgaac
     3841 ttttctaaat aaatagccct ttcccctcc
//
