LOCUS       NM_005114               7160 bp    mRNA    linear   PRI 09-JUN-2024
DEFINITION  Homo sapiens heparan sulfate-glucosamine 3-sulfotransferase 1
            (HS3ST1), mRNA.
ACCESSION   NM_005114
VERSION     NM_005114.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7160)
  AUTHORS   Smits,N.C., Kobayashi,T., Srivastava,P.K., Skopelja,S., Ivy,J.A.,
            Elwood,D.J., Stan,R.V., Tsongalis,G.J., Sellke,F.W., Gross,P.L.,
            Cole,M.D., DeVries,J.T., Kaplan,A.V., Robb,J.F., Williams,S.M. and
            Shworak,N.W.
  TITLE     HS3ST1 genotype regulates antithrombin's inflammomodulatory tone
            and associates with atherosclerosis
  JOURNAL   Matrix Biol 63, 69-90 (2017)
   PUBMED   28126521
  REMARK    GeneRIF: Compared with Hs3st1+/+ mice, Hs3st1-/- mice were more
            susceptible to LPS-induced death due to an increased sensitivity to
            TNF
REFERENCE   2  (bases 1 to 7160)
  AUTHORS   Datta,P., Li,G., Yang,B., Zhao,X., Baik,J.Y., Gemmill,T.R.,
            Sharfstein,S.T. and Linhardt,R.J.
  TITLE     Bioengineered Chinese hamster ovary cells with Golgi-targeted
            3-O-sulfotransferase-1 biosynthesize heparan sulfate with an
            antithrombin-binding site
  JOURNAL   J Biol Chem 288 (52), 37308-37318 (2013)
   PUBMED   24247246
  REMARK    GeneRIF: Golgi-targeted HS3st1 localizes in the Golgi and results
            in the formation of a single type of AT-binding site and high
            anti-factor Xa activity
REFERENCE   3  (bases 1 to 7160)
  AUTHORS   Lambert,J.C., Ibrahim-Verbaas,C.A., Harold,D., Naj,A.C., Sims,R.,
            Bellenguez,C., DeStafano,A.L., Bis,J.C., Beecham,G.W.,
            Grenier-Boley,B., Russo,G., Thorton-Wells,T.A., Jones,N.,
            Smith,A.V., Chouraki,V., Thomas,C., Ikram,M.A., Zelenika,D.,
            Vardarajan,B.N., Kamatani,Y., Lin,C.F., Gerrish,A., Schmidt,H.,
            Kunkle,B., Dunstan,M.L., Ruiz,A., Bihoreau,M.T., Choi,S.H.,
            Reitz,C., Pasquier,F., Cruchaga,C., Craig,D., Amin,N., Berr,C.,
            Lopez,O.L., De Jager,P.L., Deramecourt,V., Johnston,J.A., Evans,D.,
            Lovestone,S., Letenneur,L., Moron,F.J., Rubinsztein,D.C.,
            Eiriksdottir,G., Sleegers,K., Goate,A.M., Fievet,N.,
            Huentelman,M.W., Gill,M., Brown,K., Kamboh,M.I., Keller,L.,
            Barberger-Gateau,P., McGuiness,B., Larson,E.B., Green,R.,
            Myers,A.J., Dufouil,C., Todd,S., Wallon,D., Love,S., Rogaeva,E.,
            Gallacher,J., St George-Hyslop,P., Clarimon,J., Lleo,A., Bayer,A.,
            Tsuang,D.W., Yu,L., Tsolaki,M., Bossu,P., Spalletta,G., Proitsi,P.,
            Collinge,J., Sorbi,S., Sanchez-Garcia,F., Fox,N.C., Hardy,J., Deniz
            Naranjo,M.C., Bosco,P., Clarke,R., Brayne,C., Galimberti,D.,
            Mancuso,M., Matthews,F., Moebus,S., Mecocci,P., Del Zompo,M.,
            Maier,W., Hampel,H., Pilotto,A., Bullido,M., Panza,F., Caffarra,P.,
            Nacmias,B., Gilbert,J.R., Mayhaus,M., Lannefelt,L., Hakonarson,H.,
            Pichler,S., Carrasquillo,M.M., Ingelsson,M., Beekly,D., Alvarez,V.,
            Zou,F., Valladares,O., Younkin,S.G., Coto,E., Hamilton-Nelson,K.L.,
            Gu,W., Razquin,C., Pastor,P., Mateo,I., Owen,M.J., Faber,K.M.,
            Jonsson,P.V., Combarros,O., O'Donovan,M.C., Cantwell,L.B.,
            Soininen,H., Blacker,D., Mead,S., Mosley,T.H. Jr., Bennett,D.A.,
            Harris,T.B., Fratiglioni,L., Holmes,C., de Bruijn,R.F.,
            Passmore,P., Montine,T.J., Bettens,K., Rotter,J.I., Brice,A.,
            Morgan,K., Foroud,T.M., Kukull,W.A., Hannequin,D., Powell,J.F.,
            Nalls,M.A., Ritchie,K., Lunetta,K.L., Kauwe,J.S., Boerwinkle,E.,
            Riemenschneider,M., Boada,M., Hiltuenen,M., Martin,E.R.,
            Schmidt,R., Rujescu,D., Wang,L.S., Dartigues,J.F., Mayeux,R.,
            Tzourio,C., Hofman,A., Nothen,M.M., Graff,C., Psaty,B.M., Jones,L.,
            Haines,J.L., Holmans,P.A., Lathrop,M., Pericak-Vance,M.A.,
            Launer,L.J., Farrer,L.A., van Duijn,C.M., Van Broeckhoven,C.,
            Moskvina,V., Seshadri,S., Williams,J., Schellenberg,G.D. and
            Amouyel,P.
  CONSRTM   European Alzheimer's Disease Initiative (EADI); Genetic and
            Environmental Risk in Alzheimer's Disease; Alzheimer's Disease
            Genetic Consortium; Cohorts for Heart and Aging Research in Genomic
            Epidemiology
  TITLE     Meta-analysis of 74,046 individuals identifies 11 new
            susceptibility loci for Alzheimer's disease
  JOURNAL   Nat Genet 45 (12), 1452-1458 (2013)
   PUBMED   24162737
REFERENCE   4  (bases 1 to 7160)
  AUTHORS   Kawaguchi,T., Sumida,Y., Umemura,A., Matsuo,K., Takahashi,M.,
            Takamura,T., Yasui,K., Saibara,T., Hashimoto,E., Kawanaka,M.,
            Watanabe,S., Kawata,S., Imai,Y., Kokubo,M., Shima,T., Park,H.,
            Tanaka,H., Tajima,K., Yamada,R., Matsuda,F. and Okanoue,T.
  CONSRTM   Japan Study Group of Nonalcoholic Fatty Liver Disease
  TITLE     Genetic polymorphisms of the human PNPLA3 gene are strongly
            associated with severity of non-alcoholic fatty liver disease in
            Japanese
  JOURNAL   PLoS One 7 (6), e38322 (2012)
   PUBMED   22719876
REFERENCE   5  (bases 1 to 7160)
  AUTHORS   Edavettal,S.C., Carrick,K., Shah,R.R., Pedersen,L.C., Tropsha,A.,
            Pope,R.M. and Liu,J.
  TITLE     A conformational change in heparan sulfate 3-O-sulfotransferase-1
            is induced by binding to heparan sulfate
  JOURNAL   Biochemistry 43 (16), 4680-4688 (2004)
   PUBMED   15096036
  REMARK    GeneRIF: In this paper a conformational change is described that
            occurs in heparan sulfate 3-O-sulfotransferase-1 upon binding to
            heparan sulfate.
REFERENCE   6  (bases 1 to 7160)
  AUTHORS   Liu,J., Shworak,N.W., Sinay,P., Schwartz,J.J., Zhang,L.,
            Fritze,L.M. and Rosenberg,R.D.
  TITLE     Expression of heparan sulfate D-glucosaminyl 3-O-sulfotransferase
            isoforms reveals novel substrate specificities
  JOURNAL   J Biol Chem 274 (8), 5185-5192 (1999)
   PUBMED   9988768
REFERENCE   7  (bases 1 to 7160)
  AUTHORS   Shworak,N.W., Liu,J., Petros,L.M., Zhang,L., Kobayashi,M.,
            Copeland,N.G., Jenkins,N.A. and Rosenberg,R.D.
  TITLE     Multiple isoforms of heparan sulfate D-glucosaminyl
            3-O-sulfotransferase. Isolation, characterization, and expression
            of human cdnas and identification of distinct genomic loci
  JOURNAL   J Biol Chem 274 (8), 5170-5184 (1999)
   PUBMED   9988767
REFERENCE   8  (bases 1 to 7160)
  AUTHORS   Shworak,N.W., Liu,J., Fritze,L.M., Schwartz,J.J., Zhang,L.,
            Logeart,D. and Rosenberg,R.D.
  TITLE     Molecular cloning and expression of mouse and human cDNAs encoding
            heparan sulfate D-glucosaminyl 3-O-sulfotransferase
  JOURNAL   J Biol Chem 272 (44), 28008-28019 (1997)
   PUBMED   9346953
REFERENCE   9  (bases 1 to 7160)
  AUTHORS   Liu,J., Shworak,N.W., Fritze,L.M., Edelberg,J.M. and Rosenberg,R.D.
  TITLE     Purification of heparan sulfate D-glucosaminyl 3-O-sulfotransferase
  JOURNAL   J Biol Chem 271 (43), 27072-27082 (1996)
   PUBMED   8900198
REFERENCE   10 (bases 1 to 7160)
  AUTHORS   Razi,N. and Lindahl,U.
  TITLE     Biosynthesis of heparin/heparan sulfate. The D-glucosaminyl
            3-O-sulfotransferase reaction: target and inhibitor saccharides
  JOURNAL   J Biol Chem 270 (19), 11267-11275 (1995)
   PUBMED   7744762
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC006230.11.
            
            On Aug 31, 2019 this sequence version replaced NM_005114.3.
            
            Summary: Heparan sulfate biosynthetic enzymes are key components in
            generating a myriad of distinct heparan sulfate fine structures
            that carry out multiple biologic activities. The enzyme encoded by
            this gene is a member of the heparan sulfate biosynthetic enzyme
            family. It possesses both heparan sulfate glucosaminyl
            3-O-sulfotransferase activity, anticoagulant heparan sulfate
            conversion activity, and is a rate limiting enzyme for synthesis of
            anticoagulant heparan. This enzyme is an intraluminal Golgi
            resident protein. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14372080.4427446.1,
                                           SRR11853565.21955.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2148093 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000002596.6/ ENSP00000002596.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-196               AC006230.11        131058-131253       c
            197-7160            AC006230.11        95509-102472        c
FEATURES             Location/Qualifiers
     source          1..7160
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4p15.33"
     gene            1..7160
                     /gene="HS3ST1"
                     /gene_synonym="3OST; 3OST1"
                     /note="heparan sulfate-glucosamine 3-sulfotransferase 1"
                     /db_xref="GeneID:9957"
                     /db_xref="HGNC:HGNC:5194"
                     /db_xref="MIM:603244"
     exon            1..196
                     /gene="HS3ST1"
                     /gene_synonym="3OST; 3OST1"
                     /inference="alignment:Splign:2.1.0"
     exon            197..7160
                     /gene="HS3ST1"
                     /gene_synonym="3OST; 3OST1"
                     /inference="alignment:Splign:2.1.0"
     CDS             305..1228
                     /gene="HS3ST1"
                     /gene_synonym="3OST; 3OST1"
                     /EC_number="2.8.2.23"
                     /note="heparin-glucosamine 3-O-sulfotransferase; 3-OST-1;
                     h3-OST-1; heparan sulfate 3-O-sulfotransferase 1; heparan
                     sulfate D-glucosaminyl 3-O-sulfotransferase 1; heparan
                     sulfate (glucosamine) 3-O-sulfotransferase 1"
                     /codon_start=1
                     /product="heparan sulfate glucosamine 3-O-sulfotransferase
                     1 precursor"
                     /protein_id="NP_005105.1"
                     /db_xref="CCDS:CCDS3408.1"
                     /db_xref="GeneID:9957"
                     /db_xref="HGNC:HGNC:5194"
                     /db_xref="MIM:603244"
                     /translation="MAALLLGAVLLVAQPQLVPSRPAELGQQELLRKAGTLQDDVRDG
                     VAPNGSAQQLPQTIIIGVRKGGTRALLEMLSLHPDVAAAENEVHFFDWEEHYSHGLGW
                     YLSQMPFSWPHQLTVEKTPAYFTSPKVPERVYSMNPSIRLLLILRDPSERVLSDYTQV
                     FYNHMQKHKPYPSIEEFLVRDGRLNVDYKALNRSLYHVHMQNWLRFFPLRHIHIVDGD
                     RLIRDPFPEIQKVERFLKLSPQINASNFYFNKTKGFYCLRDSGRDRCLHESKGRAHPQ
                     VDPKLLNKLHEYFHEPNKKFFELVGRTFDWH"
     regulatory      1534..1539
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HS3ST1"
                     /gene_synonym="3OST; 3OST1"
                     /note="hexamer: AATATA"
     polyA_site      1562
                     /gene="HS3ST1"
                     /gene_synonym="3OST; 3OST1"
                     /note="major polyA site"
     regulatory      1918..1923
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HS3ST1"
                     /gene_synonym="3OST; 3OST1"
                     /note="hexamer: AATAAA"
     polyA_site      1946
                     /gene="HS3ST1"
                     /gene_synonym="3OST; 3OST1"
                     /note="major polyA site"
     regulatory      6893..6898
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HS3ST1"
                     /gene_synonym="3OST; 3OST1"
                     /note="hexamer: ATTAAA"
     polyA_site      6915
                     /gene="HS3ST1"
                     /gene_synonym="3OST; 3OST1"
     regulatory      7137..7142
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HS3ST1"
                     /gene_synonym="3OST; 3OST1"
                     /note="hexamer: GATAAA"
     polyA_site      7160
                     /gene="HS3ST1"
                     /gene_synonym="3OST; 3OST1"
ORIGIN      
        1 ctctgcgccc tggcagccag gagtcgccgc cacgacagcc gggtctcagt gggtgcctgc
       61 gccttctccc cgcccgcctg ccccgggcca tccagaaact tgctctaccc gccgcgggtg
      121 ctcggcagtg ctgcccatgg cccagcctag gagcctattt agggcgccgg acgggctgga
      181 cagaggcgcg gctcagtaat tgaaggcctg aaacgcccat gtgccactga ctaggaggct
      241 tccctgctgc ggcacttcat gacccagcgg cgcgcggccc agtgaagcca ccgtggtgtc
      301 cagcatggcc gcgctgctcc tgggcgcggt gctgctggtg gcccagcccc agctagtgcc
      361 ttcccgcccc gccgagctag gccagcagga gcttctgcgg aaagcgggga ccctccagga
      421 tgacgtccgc gatggcgtgg ccccaaacgg ctctgcccag cagttgccgc agaccatcat
      481 catcggcgtg cgcaagggcg gcacgcgcgc actgctggag atgctcagcc tgcaccccga
      541 cgtggcggcc gcggagaacg aggtccactt cttcgactgg gaggagcatt acagccacgg
      601 cttgggctgg tacctcagcc agatgccctt ctcctggcca caccagctca cagtggagaa
      661 gacccccgcg tatttcacgt cgcccaaagt gcctgagcga gtctacagca tgaacccgtc
      721 catccggctg ctgctcatcc tgcgagaccc gtcggagcgc gtgctatctg actacaccca
      781 agtgttctac aaccacatgc agaagcacaa gccctacccg tccatcgagg agttcctggt
      841 gcgcgatggc aggctcaatg tggactacaa ggccctcaac cgcagcctct accacgtgca
      901 catgcagaac tggctgcgct ttttcccgct gcgccacatc cacattgtgg acggcgaccg
      961 cctcatcagg gaccccttcc ctgagatcca aaaggtcgag aggttcctaa agctgtcgcc
     1021 gcagatcaat gcttcgaact tctactttaa caaaaccaag ggcttttact gcctgcggga
     1081 cagcggccgg gaccgctgct tacatgagtc caaaggccgg gcgcaccccc aagtcgatcc
     1141 caaactactc aataaactgc acgaatattt tcatgagcca aataagaagt tcttcgagct
     1201 tgttggcaga acatttgact ggcactgatt tgcaataagc taagctcaga aactttccta
     1261 ctgtaagttc tggtgtacat ctgaggggaa aaagaatttt aaaaaagcat ttaaggtata
     1321 atttatttgt aaaatccata aagtacttct gtacagtatt agattcacaa ttgccatata
     1381 tactagttat atttttctac ttgttaaatg gagggcattt tgtattgttt ttcatggttg
     1441 ttaacattgt gtaatatgtc tctatatgaa ggaactaaac tatttcactg aaaaaaaaaa
     1501 aaagattttt tctggagacg ctatgttttt ttgaatataa ttaacttgcc cccaactcaa
     1561 aatagctgtc tgtgttgcaa tcattgcaaa atctaaattc ttttgttact taaaaaaaca
     1621 tgtttttcat ggctattaca atcctctctc tcccctctcc tttctgcatt gctcttttta
     1681 atttttaatg tctcattgtg atcatcagat ttatttttac ttggattgta agatttatct
     1741 cctcggcgat tccttgtcat ttttggaggt cacagtttta ctcattccca tgtgtaaact
     1801 tcaagtaaac aaagcaatat tcaggatgga gaagcagtta gtgactggct agaggtctct
     1861 tgggatccat cttgacctca aagaatattt gcacatgctt ctgatctcag cagttataat
     1921 aaagaggatt aaacccttgg ccttcagaat gatcttggac ctggatactt tgccccaggt
     1981 ggcagctggc ctccagaaag ccaattagaa tagaaaagag aaactgcgaa atctatgcgt
     2041 gtcttcatcc tgtcggttct gtgaaaggct gactaaaagg gtagtagtct tttgcttagt
     2101 ctgaaagtct gcttacttcc tgaaagacac attggcatta tagacatata ttccatatgc
     2161 actgagacat aaatatgtgt atgtttcaaa atcaactcta aatttggagt cctgaatgtg
     2221 aaagaaaaag taagttccca agtgttgaag ttaaaaagag aaaagggcag agttgaaggt
     2281 agcacctaag agaaccactg tacctgcccc cagaggagtg gctggagttc agttttactt
     2341 ctgcccctga gcagccagct tgttggagat ggtcacataa acccttctta tcctcagttt
     2401 tttcatgcat tcttcatgca gtcagccttt tacagaatta aacccattgg attttaataa
     2461 aaataaaaca ttcattacag taaaagagaa aatacaaaca agcaaaataa accggcttcc
     2521 tgacatggtt tataccgcag gaaaatgaca gaaatggacg ttattaatca tctcctacac
     2581 ttaagaactc attgaaattt ctccaagcaa ccccaggatg cagatattat tatggaaatt
     2641 tcaaagagga ggaattaggc ccagagaaca tgagaaactt gcccaaggtc acattgacta
     2701 taaactcagg cttagaggct actcagagcc aaagcacagg ttatttatgt atttagtttt
     2761 tacccaactc tttctgaact caaaaggaaa agcccagact cagagccagt tcttcctgac
     2821 ctgacttaac atttcttccc aggtgccaaa tatcttccat cctaagaggt gtgtcccaga
     2881 tacaggaagg gcaagaagaa gcctgcattt ttctcagcag tgttatcagg gaagtgagtc
     2941 accttggtac gtttcagcag ctccagcctg acctgacctt agcatggcct atgccccacc
     3001 ccatatcatg aacagagagc agagaggatg ccttcctctt tagaatccat tgaagttgtc
     3061 attaagccat catctccccc agaggaagta gcagaaatat ttacagcagg cccctggtgg
     3121 acatggtact tttcaatagg cagagagcct ccctctgcag cacatcgttg gatcttgctt
     3181 ttaccatact ccttgaagag ctgaagaaat aagagggccc agcaaggtta agtcaagttt
     3241 ccaagcctgt tcaggaatat ggtgactgaa aggaaactgc aatgagagtc tctgactgaa
     3301 gaagatgctc tttgcaaact gcctgtaaat tcctgtcccc tgacagggat ggtctggtag
     3361 aagcatgaac tgtggatgca agaggcagcg gggaggagtg gaggaagaca ggaccagcac
     3421 atggaatata atctagctgc tgggactggc atagtcgatg gaggcggtgg tccagtgtaa
     3481 aatgatcagt gaatgtaaat tcaatatgtt ttcatttggg ggtgcagagt cgtggctaaa
     3541 ggaatgttca aatgagtcta ccatgagcaa gggatcagag gtcctagtgg cactatctgt
     3601 tcattcattt ttggtacaac cccagccagt cattgagaag gtacactgtg taagaaacca
     3661 tcactccaga cttacccaag gtatttattg ctagatctca ccaagcactg tcaaattcaa
     3721 aggaggccct gtagtagacc ctgggctgtc cttacagggc caaaatgcag tgactcagga
     3781 gcctaatagc acccggtccc tccaaaacat gccttctacc aattccatta acagagacag
     3841 gtgtcagtgg aaggggtggg tctcctgtcc agaccacccc ggcccgggag cagcctgggg
     3901 gatctaggtg ggtccttcca gagaggcaag tgagaacaac tgaaccaagc ctcagctatt
     3961 agatttggaa tattcacaca cttgacactc cagagtcaga acagcccctt ccctttccct
     4021 tcctcctgga atcagatagg gttgaaatcc ttttaacacc atttggttat cagctcacaa
     4081 cctgaaggtt tgcaagttta ggaaaagtga catgcctctg agcacagggt gctttactcc
     4141 ttctgtgctt ggaggcttac ccaactgaaa ggagatggag ggcaggagag gccgatgcct
     4201 agcgtgcctg ctgcttacag gggtggtgtc acaggtcacc attattcatt aaaattttac
     4261 ttttttgatc ccctaccaca tgctcaacat ggtattgagt cctcaagaat ataaaaagaa
     4321 aatacaacag gaaccagtag tcattaactg tttgttctgg actttaataa aagtacagag
     4381 ggtcttggag tacaaaggca aagacattta gctgtgtata gaatgggtgg gggaatgtgt
     4441 tccatggttt ggcattcagt tcagcaacat tcctgagaga aatcaactag cccccttata
     4501 gagaagagga aactgagatt taaaaagtgg tcctggccgg gtgtagtggc tcacgcctgt
     4561 aatcccagca ctttgggagg ccaagacagg tgaatcacga ggtcaggagt tcaagaccag
     4621 cctgaccaac atggtgaaac cccatctcta ctaaaaatac aaaaatgtgc caggcatggt
     4681 ggcgagtgcc tgtaatccca gctactcagg acactgaggc aagagaacca cttgaacctg
     4741 gcaggtggag gttgcagggg gccgagatga tgccactgca ctccagcctg ggcaacagag
     4801 caagactcca tctcaaaaaa aaaaaaaaaa aaaaaagata aattaataat aaaaaaataa
     4861 aaaagtaatc ctgacttcaa gtcatttttt tcagaagaaa ggattcttgc tcactccagg
     4921 gaaatagtcc agaaatggaa aatgttgaca tcacaaatca caaaagaagg tgaaaaatat
     4981 cagagaccct ctccagaatg tcatttctca gaaatattaa gaaatagaga atcttcccag
     5041 aggtcgcatg tcagggcagg tcacttaact tgtgggccac agagaccctg accttcctgt
     5101 gggaaggctt catggaggaa ttgccataaa gctggggcac gcacgaatgg cagtagtgtc
     5161 accagagcag ggcattctga acaagggaag cttgagacat gaagacagtg gtcacgggga
     5221 gaggctgggg gtagagggta gaggtaggca agagatttgt ctgagtggca tgtcaaatgt
     5281 gttaaactca atgagaagct actgctttcc aaggctggag tgttggaagc ttacttagca
     5341 ggcacccatg tgaatttggc tcctgtgcag gtgtatctgt tgcataaaaa tagctatcat
     5401 ttgttgcaca cctgccatgt gcttggtatt ttaaatatat tgcttctaac ctatataaca
     5461 accctaggaa gtagaaaatg ggctactaat tttacagaaa aataaactca gaaattaaag
     5521 ggcttgccca tgttgataga gccattactt cttacaaatg aaattttgtc tccgatgtct
     5581 ctgctccttt tcagtccatc tatttgtggg taagtggagg aagatataaa gcatatgcat
     5641 ctgtctatta ggcagagcct gtaagggaaa ttatgggcat ctaggatttg gagttggaca
     5701 gttagggtga tatcaagatt accttcaagc aaagcagtgg ctgggatttg atgcaacagg
     5761 gacgtgataa aacatgctcc taaccggcag cagctcattt cggaagactg agcctgagtt
     5821 aaaggccaga gcaacataac agagttctcc aaacagcaca cagtgagaga aggccaaagc
     5881 taaagaggac atcatcggat ttagctgctt cactaaagtg ttttgatgta gaaaatgacc
     5941 ttggtgcttt agttatccct gatgtcaaga cctttactct attttgccca aatttctcag
     6001 ttagataaca gcaaagagag agagccagaa gacctgggtg tgcatcctag ctgtaccaat
     6061 cactgaccat gggatcttag attcagactc tgagaacctc catttccttc tccataaaaa
     6121 tgggcagata tgtactgtac tctgttgggg gaaaactcca actcaatgag ataaagtagg
     6181 taaaaactat aaacccagtg catatgttac ttagtggtta aaaacatttg ggactttagg
     6241 agacagacca aaactggttt tgaaccagtc ggcagaaatg cgctgccttt tttcctcctc
     6301 tgcttcccag ctatgtgact gtagctattg ctgttaatgc tccatccaga gctcatttac
     6361 cagggaagtg ctctcaaccc cagctgccac aaagattgtc ttctgtacta ttttctgaag
     6421 gatttctctt agttggcagg agttttctta cctgaagatg cctgagaaat gttgtccacc
     6481 ccactcccct gctttcctag ggttagggca tgactaatga ttgactgacc catgtgggat
     6541 ggggcatggg ggtagtaggg taacagtcca ccctcattgc ctccaggcag gacaacctct
     6601 ggtgcaactc atgctcagag ctcctcgtga gatcaggctg aggctagact tctcctgaaa
     6661 ctgtactttt acttatttct tccgaaactt catccttttc ccacattacc ttactcattt
     6721 cttctgctac cctattctca gtaaatgtgt ttcccaagaa aacaggaatc ccccctcaga
     6781 ctttgcttct agaccatcca ctctaaagtc ttaaccttgc atgcatgttt tcttctctct
     6841 gtattttaca tgtataacgt gatcaaccca cggagttatt agaattcttc caattaaatg
     6901 agatatagca gagaatgagt ctattttatt ggctagcaaa tagcaaagtg ttaaattcaa
     6961 taatggctga aattgttacc aagtagtagg ctcttttatt agcttatcgg actaccaaaa
     7021 tagtataatg catcaatatc aaacttaaat ttcaacttca taaaacaatt ctgggcccaa
     7081 caaatatgaa ggtattgctc catcttatgt agcaacatct tgtatttcta ttgtttgata
     7141 aatattccac ttagaactaa
//
