LOCUS       NM_012262               6759 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens heparan sulfate 2-O-sulfotransferase 1 (HS2ST1),
            transcript variant 1, mRNA.
ACCESSION   NM_012262
VERSION     NM_012262.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6759)
  AUTHORS   Schneeberger,P.E., von Elsner,L., Barker,E.L., Meinecke,P.,
            Marquardt,I., Alawi,M., Steindl,K., Joset,P., Rauch,A.,
            Zwijnenburg,P.J.G., Weiss,M.M., Merry,C.L.R. and Kutsche,K.
  TITLE     Bi-allelic Pathogenic Variants in HS2ST1 Cause a Syndrome
            Characterized by Developmental Delay and Corpus Callosum, Skeletal,
            and Renal Abnormalities
  JOURNAL   Am J Hum Genet 107 (6), 1044-1061 (2020)
   PUBMED   33159882
  REMARK    GeneRIF: Bi-allelic Pathogenic Variants in HS2ST1 Cause a Syndrome
            Characterized by Developmental Delay and Corpus Callosum, Skeletal,
            and Renal Abnormalities.
REFERENCE   2  (bases 1 to 6759)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   3  (bases 1 to 6759)
  AUTHORS   Vijaya Kumar,A., Brezillon,S., Untereiner,V., Sockalingum,G.D.,
            Kumar Katakam,S., Mohamed,H.T., Kemper,B., Greve,B., Mohr,B.,
            Ibrahim,S.A., Goycoolea,F.M., Kiesel,L., Pavao,M.S.G., Motta,J.M.
            and Gotte,M.
  TITLE     HS2ST1-dependent signaling pathways determine breast cancer cell
            viability, matrix interactions, and invasive behavior
  JOURNAL   Cancer Sci 111 (8), 2907-2922 (2020)
   PUBMED   32573871
  REMARK    GeneRIF: HS2ST1-dependent signaling pathways determine breast
            cancer cell viability, matrix interactions, and invasive behavior.
REFERENCE   4  (bases 1 to 6759)
  AUTHORS   Prechoux,A., Halimi,C., Simorre,J.P., Lortat-Jacob,H. and Laguri,C.
  TITLE     C5-epimerase and 2-O-sulfotransferase associate in vitro to
            generate contiguous epimerized and 2-O-sulfated heparan sulfate
            domains
  JOURNAL   ACS Chem Biol 10 (4), 1064-1071 (2015)
   PUBMED   25594747
  REMARK    GeneRIF: C5-epimerase and 2-O-sulfotransferase in association
            generate extended domains of consecutive GlcNS-IdoA2S Sequence.
REFERENCE   5  (bases 1 to 6759)
  AUTHORS   Casabonne,D., Reina,O., Benavente,Y., Becker,N., Maynadie,M.,
            Foretova,L., Cocco,P., Gonzalez-Neira,A., Nieters,A., Boffetta,P.,
            Middeldorp,J.M. and de Sanjose,S.
  TITLE     Single nucleotide polymorphisms of matrix metalloproteinase 9
            (MMP9) and tumor protein 73 (TP73) interact with Epstein-Barr virus
            in chronic lymphocytic leukemia: results from the European
            case-control study EpiLymph
  JOURNAL   Haematologica 96 (2), 323-327 (2011)
   PUBMED   21048031
  REMARK    GeneRIF: Observational study of gene-disease association and
            gene-environment interaction. (HuGE Navigator)
REFERENCE   6  (bases 1 to 6759)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   7  (bases 1 to 6759)
  AUTHORS   Xu,D., Song,D., Pedersen,L.C. and Liu,J.
  TITLE     Mutational study of heparan sulfate 2-O-sulfotransferase and
            chondroitin sulfate 2-O-sulfotransferase
  JOURNAL   J Biol Chem 282 (11), 8356-8367 (2007)
   PUBMED   17227754
  REMARK    GeneRIF: analysis of differences and similarities various residues
            play in the biological roles of the HS-2OST and CS-2OST enzymes
REFERENCE   8  (bases 1 to 6759)
  AUTHORS   Aquino,R.S., Landeira-Fernandez,A.M., Valente,A.P., Andrade,L.R.
            and Mourao,P.A.
  TITLE     Occurrence of sulfated galactans in marine angiosperms:
            evolutionary implications
  JOURNAL   Glycobiology 15 (1), 11-20 (2005)
   PUBMED   15317737
REFERENCE   9  (bases 1 to 6759)
  AUTHORS   Rong,J., Habuchi,H., Kimata,K., Lindahl,U. and Kusche-Gullberg,M.
  TITLE     Expression of heparan sulphate L-iduronyl 2-O-sulphotransferase in
            human kidney 293 cells results in increased D-glucuronyl
            2-O-sulphation
  JOURNAL   Biochem J 346 Pt 2 (Pt 2), 463-468 (2000)
   PUBMED   10677367
REFERENCE   10 (bases 1 to 6759)
  AUTHORS   Muramatsu,T.
  TITLE     Essential roles of carbohydrate signals in development, immune
            response and tissue functions, as revealed by gene targeting
  JOURNAL   J Biochem 127 (2), 171-176 (2000)
   PUBMED   10731680
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL121989.12, BC025990.1,
            AB007917.1, AC093155.2 and BF431947.1.
            
            On Nov 22, 2018 this sequence version replaced NM_012262.3.
            
            Summary: Heparan sulfate biosynthetic enzymes are key components in
            generating a myriad of distinct heparan sulfate fine structures
            that carry out multiple biologic activities. This gene encodes a
            member of the heparan sulfate biosynthetic enzyme family that
            transfers sulfate to the 2 position of the iduronic acid residue of
            heparan sulfate. The disruption of this gene resulted in no kidney
            formation in knockout embryonic mice, indicating that the absence
            of this enzyme may interfere with the signaling required for kidney
            formation. Two alternatively spliced transcript variants that
            encode different proteins have been found for this gene. [provided
            by RefSeq, Aug 2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and it encodes the longer protein (isoform 1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            transcript and genomic sequence to make the sequence consistent
            with the reference genome assembly. The genomic coordinates used
            for the transcript record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660805.17875.1, AB007917.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000370550.10/ ENSP00000359581.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-17                AL121989.12        82078-82094
            18-120              BC025990.1         6-108
            121-1246            AB007917.1         1-1126
            1247-6629           AC093155.2         162239-167621
            6630-6759           BF431947.1         29-158              c
FEATURES             Location/Qualifiers
     source          1..6759
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p22.3"
     gene            1..6759
                     /gene="HS2ST1"
                     /gene_synonym="dJ604K5.2; NFSRA"
                     /note="heparan sulfate 2-O-sulfotransferase 1"
                     /db_xref="GeneID:9653"
                     /db_xref="HGNC:HGNC:5193"
                     /db_xref="MIM:604844"
     exon            1..526
                     /gene="HS2ST1"
                     /gene_synonym="dJ604K5.2; NFSRA"
                     /inference="alignment:Splign:2.1.0"
     CDS             403..1473
                     /gene="HS2ST1"
                     /gene_synonym="dJ604K5.2; NFSRA"
                     /EC_number="2.8.2.-"
                     /note="isoform 1 is encoded by transcript variant 1; 2OST;
                     2-O-sulfotransferase"
                     /codon_start=1
                     /product="heparan sulfate 2-O-sulfotransferase 1 isoform
                     1"
                     /protein_id="NP_036394.1"
                     /db_xref="CCDS:CCDS711.1"
                     /db_xref="GeneID:9653"
                     /db_xref="HGNC:HGNC:5193"
                     /db_xref="MIM:604844"
                     /translation="MGLLRIMMPPKLQLLAVVAFAVAMLFLENQIQKLEESRSKLERA
                     IARHEVREIEQRHTMDGPRQDATLDEEEDMVIIYNRVPKTASTSFTNIAYDLCAKNKY
                     HVLHINTTKNNPVMSLQDQVRFVKNITSWKEMKPGFYHGHVSYLDFAKFGVKKKPIYI
                     NVIRDPIERLVSYYYFLRFGDDYRPGLRRRKQGDKKTFDECVAEGGSDCAPEKLWLQI
                     PFFCGHSSECWNVGSRWAMDQAKYNLINEYFLVGVTEELEDFIMLLEAALPRFFRGAT
                     ELYRTGKKSHLRKTTEKKLPTKQTIAKLQQSDIWKMENEFYEFALEQFQFIRAHAVRE
                     KDGDLYILAQNFFYEKIYPKSN"
     exon            527..765
                     /gene="HS2ST1"
                     /gene_synonym="dJ604K5.2; NFSRA"
                     /inference="alignment:Splign:2.1.0"
     exon            766..851
                     /gene="HS2ST1"
                     /gene_synonym="dJ604K5.2; NFSRA"
                     /inference="alignment:Splign:2.1.0"
     exon            852..990
                     /gene="HS2ST1"
                     /gene_synonym="dJ604K5.2; NFSRA"
                     /inference="alignment:Splign:2.1.0"
     exon            991..1088
                     /gene="HS2ST1"
                     /gene_synonym="dJ604K5.2; NFSRA"
                     /inference="alignment:Splign:2.1.0"
     exon            1089..1246
                     /gene="HS2ST1"
                     /gene_synonym="dJ604K5.2; NFSRA"
                     /inference="alignment:Splign:2.1.0"
     exon            1247..6759
                     /gene="HS2ST1"
                     /gene_synonym="dJ604K5.2; NFSRA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5939..5944
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HS2ST1"
                     /gene_synonym="dJ604K5.2; NFSRA"
                     /note="hexamer: AATAAA"
     polyA_site      5962
                     /gene="HS2ST1"
                     /gene_synonym="dJ604K5.2; NFSRA"
                     /note="major polyA site"
     regulatory      6752..6757
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HS2ST1"
                     /gene_synonym="dJ604K5.2; NFSRA"
                     /note="hexamer: TATAAA"
     polyA_site      6759
                     /gene="HS2ST1"
                     /gene_synonym="dJ604K5.2; NFSRA"
ORIGIN      
        1 gcagccgcag cgccggtgga ggggcgcgcg gccgcgagca aaggagggag ggaaggaagg
       61 aagagaggga ggcgggcaag caggcgggcg cgggggtcgg ggactgaggc agtagaggga
      121 ggcgagagcc cggcagccgc ttcgcgctgt ttgctgcgcg ggcttttgga gggggcggcc
      181 gtttagtcgg ctgaggagaa gcggacacca gcggcgttgg tgatagcgcc tgggggaggg
      241 ggactggaga ggcgagaagg ggggtcgctg cggtggttct ctcgctgtcg ctctctcttt
      301 gcctcgctcc cggctcggcg ggctcctccc ggcgtctctc tcgcctccgg ggtcccgctc
      361 cccgcccccc gcggtatgtc ttgatcccga gcagcgggtt tcatggggct cctcaggatt
      421 atgatgccgc ccaagttgca gctgctggcg gtggtggcct tcgcggtggc gatgctcttc
      481 ttggaaaacc agatccagaa actggaggag tcccgctcga agctagaaag ggctattgca
      541 agacacgaag tccgagaaat tgagcagcga catacaatgg atggccctcg gcaagatgcc
      601 actttagatg aggaagagga catggtgatc atttataaca gagttcccaa aacggcaagc
      661 acttcattta ccaatatcgc ctatgacctg tgtgcaaaga ataaatacca tgtccttcat
      721 atcaacacta ccaaaaataa tccagtgatg tcattgcaag atcaggtgcg ctttgtaaag
      781 aatataactt cctggaaaga gatgaaacca ggattttatc atggacacgt ttcttacttg
      841 gattttgcaa aatttggtgt gaagaagaaa ccaatttaca ttaatgtcat aagggatcct
      901 attgagaggc tagtttctta ttattacttt ctgagatttg gagatgatta tagaccaggg
      961 ttacggagac gaaaacaagg agacaaaaag acctttgatg aatgtgtagc agaaggtggc
     1021 tcagactgtg ctccagagaa gctctggctt caaatcccgt tcttctgtgg ccatagctcc
     1081 gaatgctgga atgtgggaag caggtgggct atggatcaag ccaagtataa cctaattaat
     1141 gaatattttc tggtgggagt tactgaagaa cttgaagatt ttatcatgtt attggaggca
     1201 gcattgcccc ggtttttcag gggtgctact gaactctatc gcacaggaaa gaaatctcat
     1261 cttaggaaaa ccacagagaa gaaactcccc actaaacaaa ccattgcaaa actacagcaa
     1321 tctgatattt ggaaaatgga gaatgagttc tatgaatttg cactagagca gttccaattc
     1381 atcagagccc atgccgttcg agaaaaagat ggagacctct acatcctcgc acaaaacttt
     1441 ttctatgaaa agatttaccc taagtcgaac tgagtataag gtgtgactat tggattcttg
     1501 aactaaaatt tgaccctgtc ttcacctttg ttctcagctc cacagtctgg attgctgaca
     1561 gtaggtgtat atgacaattt gtattgagcc aaattaggaa acagacagta acgtcaagga
     1621 agtagatact ggctggcatt gtcagtgttc taagtttcag gcatttttat ttttcctggc
     1681 taaacgttgg tgaaagttat aacctcctgc ctgggagaaa atatacatca cctaaaatga
     1741 acttatggca ggtctaatca aaaggctaaa tacaatttca gaaaaggttc tgatactctt
     1801 gtttttgata aagcattttt tcaactaacc atgaattaag atgagtccat ttgcctcttc
     1861 tgccttcact gagggtttgg gttatacacc tctactgaat tgtgttaata actgtttggc
     1921 agtgtgtact ttgtttttgt gagtcatgtc tcatgaaatt tattggaatg tttaatcata
     1981 tttgctaaga aatgtttctg ctgtagttgg atttgcccat atttatgtag gtggttttaa
     2041 ttttttaaat ggtgattagt gttaaaaatc aatttaaatc atgactaata tggtaaaaag
     2101 ataaagcatc aaagcagtat ttctcattcc tgcctcctca atatctaata ctgggaagat
     2161 acttcaaaga atattgagat tgtctgaagt tttagttaag attttcacac attaatatca
     2221 aaaaagtaag tttagtattt gtttctccat gggttatttg taaagctgta aactgagata
     2281 tcggtgactc cgtattatga ctccattagt gagctgtggt atgggtagga ttttcctact
     2341 tcttctgtac ttttacctgt agactatttt tactaaggtg ctttataatg tgttttaaag
     2401 cattgcattt acaaaacaag gaaaatgctg taaatattgc atattttatg tatttggacc
     2461 aaaaggttac aagtaattag acaaaagtgg ttttgcacca attttatgtc aagtaaaacc
     2521 atcagaccta ctgttcttgt atttctcatt taactttact gttaagacat cactgaaatg
     2581 aacttcagta agctttcaat tttgatacac agttcattat tcataacttg aggcagtaat
     2641 tacagtggaa tgagtactgg acaaggagtc aaaaaacttg atttcaggtc ctagctctag
     2701 cacttacagc tgtgtgatct tgggcaagtc acttaacctc tctttgcctc aatttcctca
     2761 tcttgaaatg aggataataa tacctgctgt acctacctca cagggctgtt gtgaggatta
     2821 aatgagatgg catgtgaaag cactttgaaa attgtaaagc gctatgtaaa tgtaaggtat
     2881 tatagaaaca tctttaacat atagtttcat accattcatt ttttaacaaa gaaagggaaa
     2941 agtctgcttg taagctggtt gaaaaagtta atcttgatat aaatttgtgt ttgataaata
     3001 tcctctcagt gttttatctt ccatgtttca acaactattg aaatatgaaa tgcctgtgaa
     3061 ctcttaaagc ttcatgagca gctgcttgag ttcaggaagt tcactgttag aaataggctt
     3121 tgttagctga ctagggtcag ggaaactttt ctcttcaaat ttgaaagctg tttctgtttt
     3181 cattttacat tattattcag aaatggtagc tattctatac ctatggttta agtaaatatt
     3241 tctgaataag gcttcaccat actgtaagca ttttaggtag attgccttaa aggttatggg
     3301 agggcatgag ggaacacttc ttatgagaaa acatttataa acaaaagaaa catttataaa
     3361 ctaaagaaaa actaaaagaa tgacagaaca atcatcttag caccctttcc tcacaataat
     3421 ataaaaatat taaaagaaca taggcaggct ttttttaaat ttggcttttt tctttccttt
     3481 tttcaaattg acttttatag gtatttcctg aaagtgtata caaattattt cctcgcccaa
     3541 aataaagcac cacttcaagg tgtggtttga cattacatgc taatgaacaa acccagtatg
     3601 caagttattc ttgcaccaca tgctcaaatc ttcttgaggt gcattaactc ttttaggtaa
     3661 ctagagcagt acttggtgaa ctagatcagg aggtcagtaa actttctgtg gaagggccag
     3721 agagtaaata ttttaggctt tgcagcccat acggtctctg tcacagctag tcaaccctgc
     3781 cattttacca caaaagcagc aatagacatt atgtaaacaa atgagcacag ttatgttcca
     3841 ataaaacttt atttacaaaa acagatgaca tcccagatgc agaccatggg caaccaacca
     3901 ttgcactggc caaatcatta tttatggaga aatcctcttt gtgtctctac tctagatgcc
     3961 taaaagagtt tatatacttc taaaagctcc taacttatat ccaaagaatt gctttctgat
     4021 tcgtgtagtc tctcccacag attcataaac ttttatgact tatattgttt ccaggtgggc
     4081 atggtttatt tcccagttta acagttcaga ataggggcat ttattttatc atattttagg
     4141 gtgggttagg agtatccttt ctggagactg agaaaggggt gtatttaatt ccatcaggtc
     4201 cagtacagta ctaggagtca taatacttta taatcaatta aataaataga accactgaga
     4261 caataatgta tttttttaaa gtggcaaatg tggttttctt ttttcagcct ttgcgctttt
     4321 tcagtatttt gaccataggg agataatttt tttataatac aaaagtaacc acttggaatt
     4381 ttaaagataa tgttatgtgt gtatgtgaaa tatatataca tatatatata tatgtttatt
     4441 tcctaaaaga agaaaagata cctttctgtt caacttgtat caactcctct tttctaattg
     4501 ctgtgaaatg gcaactgttg ataaattatt gtgattgttt taaaatctaa tgggaagtaa
     4561 aatatatttt gattttaccc agcttaatct gtaaagtagc acttaaatat atctgatagc
     4621 aacacttaag atattgcatg gggattactt tcctatcatc catatgcatt tgtgcaactt
     4681 caaacatatt gggtgcttct gaattcctga tgattggatt taagctattg aaaattggat
     4741 aatttaaact taatgatttt tataattttc tgatcttaaa atttggttaa tgcctataat
     4801 ctgttgcttt ttctcaatat gtgtcctatt ggaaattcct caaatcgttg gtgccatcag
     4861 tgatttacaa acaatatttt gatattgcag atgacttgct tactgtattt gcattgttag
     4921 aaaacagttt gtagacaatg attctttttt aataaaatca aataattcta aaagtgctag
     4981 agaatttaac taaaagctgg ttcccaaatg catagctggc attttaattt aaattcaaat
     5041 ctacatagag aacatctgtg taaatcatct aactggattt ttccattggt cattcccaaa
     5101 cacacctatg gtcctagaat ccttaagaga agcaccctgt aaccttttat gtggtttgcc
     5161 tttaagaggc ccaggtgctt ctcctttatg atttgagttg gcctcttcat aaattagtgc
     5221 tgtttacttt cagaggaagc agagaagttg ctgttatgtt tttgcatccg tttaccctat
     5281 gcaaagttgc tgtatgatgc caactaaact actctttagg cagccttctg aggagaaaag
     5341 caaccctgtt tcaaatccac tgccaattca gctcctctgg agtggagctt tctgatttct
     5401 tggagcagga attttagaga ttgaaatgaa tgatcattta gtcagattta tcctgtaatt
     5461 tcatgcagct ttgtggcctt tgcagtacta tttataaaat ggaccctgat ggtgatgaac
     5521 tctttagaac gcattactgt taagcctgtg ttgagacatt gatgctgtct atctcatttt
     5581 ttagacagtt tttgtagctt tctattgaga gtcaggtatg tgagcatctc tgaagcagtg
     5641 ttgaatgtaa ttttcggaaa catggattgt gtattttgac ttttatttta taaatacaca
     5701 gctcaacagt gccttttttt tccctcatag tcctgttgga agatgctcac tactttctct
     5761 cttctctctc cctgccctcc cccactccat tcagttgatt catttatgca aattctgttt
     5821 ccaacttgaa accattttgt cacatctgtt ggagagataa tcactccttt tccttaacat
     5881 tctgccagct ttctgatgtt gaagtgtttc agttgactac ctgatgcaaa agctataaaa
     5941 taaacagtgg gaaggggaaa aattggtgtc ctgttttaat attttctttt gtagccttga
     6001 cactgatgga cattttccaa gctgactcag tgttcagtgt caacttaact ctcagatagt
     6061 gttgccatca agaaagcatg caacatcatt ggtttctaat gattttatgg cttgtgacaa
     6121 tattttatct ggactgacat gcctctgctg cttttgcttt gtacttcatt gctggtaata
     6181 aaatttcaga tggaaaactt acaaaatata tacttaatta gaagaaaaaa atagagaaag
     6241 ggctattaga attaaaaaaa tttgaaagta acttaatcta acatttatgg cacagtttgg
     6301 acatatccat aatttttttt gggaacacac atttctgatt ttttttttcc cccttaaaga
     6361 agaaagtctc aattccattg attttcaatt cttagccact ggctcattgc tttgagcaat
     6421 gcttgattga ttctatttat attatatgat attgggttga taaaatacca gttcaatgat
     6481 gagttttctt aacagaattt ggtttgtact tgcagtggct gaacaaagag catggcttga
     6541 gaatcaaagg gatctgcatt tagcaatgtg atgtcagtaa atggacataa caggattgtt
     6601 gtaaaggttg ggcatgatgt atgcaaagta ctggccaggg tagactaata actgatggca
     6661 tttatatgct gtgctggaat attgttacca agctgatgtg ccgttctcac cctgcagaat
     6721 actggttttg tcatttcata aatgatattt ttataaata
//
