LOCUS       NM_001384133            1746 bp    mRNA    linear   PRI 17-MAR-2024
DEFINITION  Homo sapiens hepsin (HPN), transcript variant 4, mRNA.
ACCESSION   NM_001384133
VERSION     NM_001384133.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Belitskin,D., Munne,P., Pant,S.M., Anttila,J.M., Suleymanova,I.,
            Belitskina,K., Kirchhofer,D., Janetka,J., Kasper,T., Jalil,S.,
            Pouwels,J., Tervonen,T.A. and Klefstrom,J.
  TITLE     Hepsin promotes breast tumor growth signaling via the TGFbeta-EGFR
            axis
  JOURNAL   Mol Oncol 18 (3), 547-561 (2024)
   PUBMED   37872868
  REMARK    GeneRIF: Hepsin promotes breast tumor growth signaling via the
            TGFbeta-EGFR axis.
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Bergeron,J.J.M. and Thomas,D.Y.
  TITLE     N-glycosylation mediated folding and quality control in serine
            proteases of the hepsin family
  JOURNAL   FEBS J 290 (16), 3963-3965 (2023)
   PUBMED   37013685
  REMARK    GeneRIF: N-glycosylation mediated folding and quality control in
            serine proteases of the hepsin family.
REFERENCE   3  (bases 1 to 1746)
  AUTHORS   Sun,S., Hu,K., Wang,L., Liu,M., Zhang,Y., Dong,N. and Wu,Q.
  TITLE     Spatial position is a key determinant of N-glycan functionality of
            the scavenger receptor cysteine-rich domain of human hepsin
  JOURNAL   FEBS J 290 (16), 3966-3982 (2023)
   PUBMED   36802168
  REMARK    GeneRIF: Spatial position is a key determinant of N-glycan
            functionality of the scavenger receptor cysteine-rich domain of
            human hepsin.
REFERENCE   4  (bases 1 to 1746)
  AUTHORS   Li,S., Wang,L., Sun,S. and Wu,Q.
  TITLE     Hepsin: a multifunctional transmembrane serine protease in
            pathobiology
  JOURNAL   FEBS J 288 (18), 5252-5264 (2021)
   PUBMED   33300264
  REMARK    GeneRIF: Hepsin: a multifunctional transmembrane serine protease in
            pathobiology.
            Review article
REFERENCE   5  (bases 1 to 1746)
  AUTHORS   Li,Y., Yu,Z., Zhao,X. and Shen,S.H.
  TITLE     Identification and characterization of hepsin/-TM, a
            non-transmembrane hepsin isoform
  JOURNAL   Biochim Biophys Acta 1681 (2-3), 157-165 (2005)
   PUBMED   15627507
REFERENCE   6  (bases 1 to 1746)
  AUTHORS   Somoza,J.R., Ho,J.D., Luong,C., Ghate,M., Sprengeler,P.A.,
            Mortara,K., Shrader,W.D., Sperandio,D., Chan,H., McGrath,M.E. and
            Katz,B.A.
  TITLE     The structure of the extracellular region of human hepsin reveals a
            serine protease domain and a novel scavenger receptor cysteine-rich
            (SRCR) domain
  JOURNAL   Structure 11 (9), 1123-1131 (2003)
   PUBMED   12962630
REFERENCE   7  (bases 1 to 1746)
  AUTHORS   Kazama,Y., Hamamoto,T., Foster,D.C. and Kisiel,W.
  TITLE     Hepsin, a putative membrane-associated serine protease, activates
            human factor VII and initiates a pathway of blood coagulation on
            the cell surface leading to thrombin formation
  JOURNAL   J Biol Chem 270 (1), 66-72 (1995)
   PUBMED   7814421
REFERENCE   8  (bases 1 to 1746)
  AUTHORS   Torres-Rosado,A., O'Shea,K.S., Tsuji,A., Chou,S.H. and Kurachi,K.
  TITLE     Hepsin, a putative cell-surface serine protease, is required for
            mammalian cell growth
  JOURNAL   Proc Natl Acad Sci U S A 90 (15), 7181-7185 (1993)
   PUBMED   8346233
REFERENCE   9  (bases 1 to 1746)
  AUTHORS   Tsuji,A., Torres-Rosado,A., Arai,T., Le Beau,M.M., Lemons,R.S.,
            Chou,S.H. and Kurachi,K.
  TITLE     Hepsin, a cell membrane-associated protease. Characterization,
            tissue distribution, and gene localization
  JOURNAL   J Biol Chem 266 (25), 16948-16953 (1991)
   PUBMED   1885621
REFERENCE   10 (bases 1 to 1746)
  AUTHORS   Leytus,S.P., Loeb,K.R., Hagen,F.S., Kurachi,K. and Davie,E.W.
  TITLE     A novel trypsin-like serine protease (hepsin) with a putative
            transmembrane domain expressed by human liver and hepatoma cells
  JOURNAL   Biochemistry 27 (3), 1067-1074 (1988)
   PUBMED   2835076
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC020907.6.
            
            Summary: This gene encodes a type II transmembrane serine protease
            that may be involved in diverse cellular functions, including blood
            coagulation and the maintenance of cell morphology. Expression of
            the encoded protein is associated with the growth and progression
            of cancers, particularly prostate cancer. The protein is cleaved
            into a catalytic serine protease chain and a non-catalytic
            scavenger receptor cysteine-rich chain, which associate via a
            single disulfide bond. Alternative splicing results in multiple
            transcript variants. [provided by RefSeq, Jan 2013].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC025716.1, AK315655.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968832, SAMEA1970526
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000672452.2/ ENSP00000500664.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-152               AC020907.6         55224-55375
            153-222             AC020907.6         55956-56025
            223-324             AC020907.6         62793-62894
            325-366             AC020907.6         62978-63019
            367-496             AC020907.6         73176-73305
            497-619             AC020907.6         73377-73499
            620-660             AC020907.6         73632-73672
            661-826             AC020907.6         73850-74015
            827-1017            AC020907.6         74130-74320
            1018-1113           AC020907.6         78753-78848
            1114-1256           AC020907.6         79042-79184
            1257-1421           AC020907.6         79371-79535
            1422-1746           AC020907.6         79752-80076
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.11"
     gene            1..1746
                     /gene="HPN"
                     /gene_synonym="TMPRSS1"
                     /note="hepsin"
                     /db_xref="GeneID:3249"
                     /db_xref="HGNC:HGNC:5155"
                     /db_xref="MIM:142440"
     exon            1..152
                     /gene="HPN"
                     /gene_synonym="TMPRSS1"
                     /inference="alignment:Splign:2.1.0"
     exon            153..222
                     /gene="HPN"
                     /gene_synonym="TMPRSS1"
                     /inference="alignment:Splign:2.1.0"
     CDS             207..1460
                     /gene="HPN"
                     /gene_synonym="TMPRSS1"
                     /EC_number="3.4.21.106"
                     /note="transmembrane protease serine 1; testicular tissue
                     protein Li 85; serine protease hepsin"
                     /codon_start=1
                     /product="serine protease hepsin precursor"
                     /protein_id="NP_001371062.1"
                     /db_xref="CCDS:CCDS32993.1"
                     /db_xref="GeneID:3249"
                     /db_xref="HGNC:HGNC:5155"
                     /db_xref="MIM:142440"
                     /translation="MAQKEGGRTVPCCSRPKVAALTAGTLLLLTAIGAASWAIVAVLL
                     RSDQEPLYPVQVSSADARLMVFDKTEGTWRLLCSSRSNARVAGLSCEEMGFLRALTHS
                     ELDVRTAGANGTSGFFCVDEGRLPHTQRLLEVISVCDCPRGRFLAAICQDCGRRKLPV
                     DRIVGGRDTSLGRWPWQVSLRYDGAHLCGGSLLSGDWVLTAAHCFPERNRVLSRWRVF
                     AGAVAQASPHGLQLGVQAVVYHGGYLPFRDPNSEENSNDIALVHLSSPLPLTEYIQPV
                     CLPAAGQALVDGKICTVTGWGNTQYYGQQAGVLQEARVPIISNDVCNGADFYGNQIKP
                     KMFCAGYPEGGIDACQGDSGGPFVCEDSISRTPRWRLCGIVSWGTGCALAQKPGVYTK
                     VSDFREWIFQAIKTHSEASGMVTQL"
     exon            223..324
                     /gene="HPN"
                     /gene_synonym="TMPRSS1"
                     /inference="alignment:Splign:2.1.0"
     exon            325..366
                     /gene="HPN"
                     /gene_synonym="TMPRSS1"
                     /inference="alignment:Splign:2.1.0"
     exon            367..496
                     /gene="HPN"
                     /gene_synonym="TMPRSS1"
                     /inference="alignment:Splign:2.1.0"
     exon            497..619
                     /gene="HPN"
                     /gene_synonym="TMPRSS1"
                     /inference="alignment:Splign:2.1.0"
     exon            620..660
                     /gene="HPN"
                     /gene_synonym="TMPRSS1"
                     /inference="alignment:Splign:2.1.0"
     exon            661..826
                     /gene="HPN"
                     /gene_synonym="TMPRSS1"
                     /inference="alignment:Splign:2.1.0"
     exon            827..1017
                     /gene="HPN"
                     /gene_synonym="TMPRSS1"
                     /inference="alignment:Splign:2.1.0"
     exon            1018..1113
                     /gene="HPN"
                     /gene_synonym="TMPRSS1"
                     /inference="alignment:Splign:2.1.0"
     exon            1114..1256
                     /gene="HPN"
                     /gene_synonym="TMPRSS1"
                     /inference="alignment:Splign:2.1.0"
     exon            1257..1421
                     /gene="HPN"
                     /gene_synonym="TMPRSS1"
                     /inference="alignment:Splign:2.1.0"
     exon            1422..1746
                     /gene="HPN"
                     /gene_synonym="TMPRSS1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1726..1731
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HPN"
                     /gene_synonym="TMPRSS1"
                     /note="hexamer: AATAAA"
     polyA_site      1746
                     /gene="HPN"
                     /gene_synonym="TMPRSS1"
                     /note="major polyA site"
ORIGIN      
        1 ggtgaggcag cctggcctag caggccccac gccaccgcct ctgcctccag gccgcccgct
       61 gctgcggggc caccatgctc ctgcccaggc ctggagactg acccgacccc ggcactacct
      121 cgaggctccg cccccacctg ctggacccca gggtcccacc ctggcccagg aggtcagcca
      181 gggaatcatt aacaagaggc agtgacatgg cgcagaagga gggtggccgg actgtgccat
      241 gctgctccag acccaaggtg gcagctctca ctgcggggac cctgctactt ctgacagcca
      301 tcggggcggc atcctgggcc attgtggctg ttctcctcag gagtgaccag gagccgctgt
      361 acccagtgca ggtcagctct gcggacgctc ggctcatggt ctttgacaag acggaaggga
      421 cgtggcggct gctgtgctcc tcgcgctcca acgccagggt agccggactc agctgcgagg
      481 agatgggctt cctcagggca ctgacccact ccgagctgga cgtgcgaacg gcgggcgcca
      541 atggcacgtc gggcttcttc tgtgtggacg aggggaggct gccccacacc cagaggctgc
      601 tggaggtcat ctccgtgtgt gattgcccca gaggccgttt cttggccgcc atctgccaag
      661 actgtggccg caggaagctg cccgtggacc gcatcgtggg aggccgggac accagcttgg
      721 gccggtggcc gtggcaagtc agccttcgct atgatggagc acacctctgt gggggatccc
      781 tgctctccgg ggactgggtg ctgacagccg cccactgctt cccggagcgg aaccgggtcc
      841 tgtcccgatg gcgagtgttt gccggtgccg tggcccaggc ctctccccac ggtctgcagc
      901 tgggggtgca ggctgtggtc taccacgggg gctatcttcc ctttcgggac cccaacagcg
      961 aggagaacag caacgatatt gccctggtcc acctctccag tcccctgccc ctcacagaat
     1021 acatccagcc tgtgtgcctc ccagctgccg gccaggccct ggtggatggc aagatctgta
     1081 ccgtgacggg ctggggcaac acgcagtact atggccaaca ggccggggta ctccaggagg
     1141 ctcgagtccc cataatcagc aatgatgtct gcaatggcgc tgacttctat ggaaaccaga
     1201 tcaagcccaa gatgttctgt gctggctacc ccgagggtgg cattgatgcc tgccagggcg
     1261 acagcggtgg tccctttgtg tgtgaggaca gcatctctcg gacgccacgt tggcggctgt
     1321 gtggcattgt gagttggggc actggctgtg ccctggccca gaagccaggc gtctacacca
     1381 aagtcagtga cttccgggag tggatcttcc aggccataaa gactcactcc gaagccagcg
     1441 gcatggtgac ccagctctga ccggtggctt ctcgctgcgc agcctccagg gcccgaggtg
     1501 atcccggtgg tgggatccac gctgggccta ggatgggacg tttttcttct tgggcccggt
     1561 ccacaggtcc aaggacaccc tccctccagg gtcctctctt ccacagtggc gggcccactc
     1621 agccccgaga ccacccaacc tcaccctcct gacccccatg taaatattgt tctgctgtct
     1681 gggactcctg tctaggtgcc cctgatgacg ggatgctctt taaataataa agatggtttt
     1741 gattaa
//
