LOCUS       NM_000859               4530 bp    mRNA    linear   PRI 03-SEP-2024
DEFINITION  Homo sapiens 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR),
            transcript variant 1, mRNA.
ACCESSION   NM_000859
VERSION     NM_000859.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4530)
  AUTHORS   Pierzchlinska,A., Slawek,J., Kwasniak-Butowska,M., Malinowski,D.,
            Komaniecka,N., Mak,M., Czerkawska,A., Kukowka,A. and Bialecka,M.
  TITLE     Genetic Polymorphisms in the HMGCR Gene and Associations with
            Cognitive Decline in Parkinson's Disease Patients
  JOURNAL   Int J Mol Sci 25 (16), 8964 (2024)
   PUBMED   39201649
  REMARK    GeneRIF: Genetic Polymorphisms in the HMGCR Gene and Associations
            with Cognitive Decline in Parkinson's Disease Patients.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4530)
  AUTHORS   Wang,J., Kunze,M., Villoria-Gonzalez,A., Weinhofer,I. and Berger,J.
  TITLE     Peroxisomal Localization of a Truncated HMG-CoA Reductase under Low
            Cholesterol Conditions
  JOURNAL   Biomolecules 14 (2), 244 (2024)
   PUBMED   38397481
  REMARK    GeneRIF: Peroxisomal Localization of a Truncated HMG-CoA Reductase
            under Low Cholesterol Conditions.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4530)
  AUTHORS   Li,Y., Li,J., Tang,X., Xu,J., Liu,R., Jiang,L., Tian,J., Zhang,Y.,
            Wang,D., Sun,K., Xu,B., Zhao,W., Hui,R., Gao,R., Song,L., Yuan,J.
            and Zhao,X.
  TITLE     Association of NPC1L1 and HMGCR gene polymorphisms with coronary
            artery calcification in patients with premature triple-vessel
            coronary disease
  JOURNAL   BMC Med Genomics 17 (1), 22 (2024)
   PUBMED   38233830
  REMARK    GeneRIF: Association of NPC1L1 and HMGCR gene polymorphisms with
            coronary artery calcification in patients with premature
            triple-vessel coronary disease.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4530)
  AUTHORS   Perrone,B., Ruffo,P., Augimeri,G., Sisci,D., Sinicropi,M.S.,
            Tripepi,G., Mammi,C., Bonofiglio,D. and Conforti,F.L.
  TITLE     Association between HMGCR, CRP, and CETP gene polymorphisms and
            metabolic/inflammatory serum profile in healthy adolescents
  JOURNAL   J Transl Med 21 (1), 718 (2023)
   PUBMED   37833739
  REMARK    GeneRIF: Association between HMGCR, CRP, and CETP gene
            polymorphisms and metabolic/inflammatory serum profile in healthy
            adolescents.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4530)
  AUTHORS   Li,J., Tang,X., Xu,J., Liu,R., Jiang,L., Xu,L., Tian,J., Feng,X.,
            Wu,Y., Zhang,Y., Wang,D., Sun,K., Xu,B., Zhao,W., Hui,R., Gao,R.,
            Song,L., Yuan,J. and Zhao,X.
  TITLE     HMGCR gene polymorphism is associated with residual cholesterol
            risk in premature triple-vessel disease patients treated with
            moderate-intensity statins
  JOURNAL   BMC Cardiovasc Disord 23 (1), 317 (2023)
   PUBMED   37355634
  REMARK    GeneRIF: HMGCR gene polymorphism is associated with residual
            cholesterol risk in premature triple-vessel disease patients
            treated with moderate-intensity statins.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4530)
  AUTHORS   Hodge,V.J., Gould,S.J., Subramani,S., Moser,H.W. and Krisans,S.K.
  TITLE     Normal cholesterol synthesis in human cells requires functional
            peroxisomes
  JOURNAL   Biochem Biophys Res Commun 181 (2), 537-541 (1991)
   PUBMED   1755834
REFERENCE   7  (bases 1 to 4530)
  AUTHORS   Ramharack,R., Tam,S.P. and Deeley,R.G.
  TITLE     Characterization of three distinct size classes of human
            3-hydroxy-3-methylglutaryl coenzyme A reductase mRNA: expression of
            the transcripts in hepatic and nonhepatic cells
  JOURNAL   DNA Cell Biol 9 (9), 677-690 (1990)
   PUBMED   1979742
REFERENCE   8  (bases 1 to 4530)
  AUTHORS   Clarke,P.R. and Hardie,D.G.
  TITLE     Regulation of HMG-CoA reductase: identification of the site
            phosphorylated by the AMP-activated protein kinase in vitro and in
            intact rat liver
  JOURNAL   EMBO J 9 (8), 2439-2446 (1990)
   PUBMED   2369897
REFERENCE   9  (bases 1 to 4530)
  AUTHORS   Luskey,K.L. and Stevens,B.
  TITLE     Human 3-hydroxy-3-methylglutaryl coenzyme A reductase. Conserved
            domains responsible for catalytic activity and sterol-regulated
            degradation
  JOURNAL   J Biol Chem 260 (18), 10271-10277 (1985)
   PUBMED   2991281
REFERENCE   10 (bases 1 to 4530)
  AUTHORS   Humphries,S.E., Tata,F., Henry,I., Barichard,F., Holm,M., Junien,C.
            and Williamson,R.
  TITLE     The isolation, characterisation, and chromosomal assignment of the
            gene for human 3-hydroxy-3-methylglutaryl coenzyme A reductase,
            (HMG-CoA reductase)
  JOURNAL   Hum Genet 71 (3), 254-258 (1985)
   PUBMED   2998972
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from M62627.1, BC033692.1, M11058.1,
            CN278665.1 and AA648735.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_000859.2.
            
            Summary: HMG-CoA reductase is the rate-limiting enzyme for
            cholesterol synthesis and is regulated via a negative feedback
            mechanism mediated by sterols and non-sterol metabolites derived
            from mevalonate, the product of the reaction catalyzed by
            reductase. Normally in mammalian cells this enzyme is suppressed by
            cholesterol derived from the internalization and degradation of low
            density lipoprotein (LDL) via the LDL receptor. Competitive
            inhibitors of the reductase induce the expression of LDL receptors
            in the liver, which in turn increases the catabolism of plasma LDL
            and lowers the plasma concentration of cholesterol, an important
            determinant of atherosclerosis. Alternatively spliced transcript
            variants encoding different isoforms have been found for this gene.
            [provided by RefSeq, Aug 2008].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longer isoform (1). Variants 1 and 3
            both encode the same isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803616.216274.1,
                                           SRR1803616.157341.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000287936.9/ ENSP00000287936.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-45                M62627.1           301-345
            46-1667             BC033692.1         34-1655
            1668-2877           M11058.1           1614-2823
            2878-3852           BC033692.1         2707-3681
            3853-4524           CN278665.1         5-676               c
            4525-4530           AA648735.1         8-13                c
FEATURES             Location/Qualifiers
     source          1..4530
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q13.3"
     gene            1..4530
                     /gene="HMGCR"
                     /gene_synonym="LDLCQ3; LGMDR28; MYPLG"
                     /note="3-hydroxy-3-methylglutaryl-CoA reductase"
                     /db_xref="GeneID:3156"
                     /db_xref="HGNC:HGNC:5006"
                     /db_xref="MIM:142910"
     exon            1..81
                     /gene="HMGCR"
                     /gene_synonym="LDLCQ3; LGMDR28; MYPLG"
                     /inference="alignment:Splign:2.1.0"
     exon            82..269
                     /gene="HMGCR"
                     /gene_synonym="LDLCQ3; LGMDR28; MYPLG"
                     /inference="alignment:Splign:2.1.0"
     CDS             105..2771
                     /gene="HMGCR"
                     /gene_synonym="LDLCQ3; LGMDR28; MYPLG"
                     /EC_number="1.1.1.34"
                     /note="isoform 1 is encoded by transcript variant 1;
                     3-hydroxy-3-methylglutaryl-Coenzyme A reductase;
                     hydroxymethylglutaryl-CoA reductase;
                     3-hydroxy-3-methylglutaryl CoA reductase (NADPH); HMG-CoA
                     reductase"
                     /codon_start=1
                     /product="3-hydroxy-3-methylglutaryl-Coenzyme A reductase
                     isoform 1"
                     /protein_id="NP_000850.1"
                     /db_xref="CCDS:CCDS4027.1"
                     /db_xref="GeneID:3156"
                     /db_xref="HGNC:HGNC:5006"
                     /db_xref="MIM:142910"
                     /translation="MLSRLFRMHGLFVASHPWEVIVGTVTLTICMMSMNMFTGNNKIC
                     GWNYECPKFEEDVLSSDIIILTITRCIAILYIYFQFQNLRQLGSKYILGIAGLFTIFS
                     SFVFSTVVIHFLDKELTGLNEALPFFLLLIDLSRASTLAKFALSSNSQDEVRENIARG
                     MAILGPTFTLDALVECLVIGVGTMSGVRQLEIMCCFGCMSVLANYFVFMTFFPACVSL
                     VLELSRESREGRPIWQLSHFARVLEEEENKPNPVTQRVKMIMSLGLVLVHAHSRWIAD
                     PSPQNSTADTSKVSLGLDENVSKRIEPSVSLWQFYLSKMISMDIEQVITLSLALLLAV
                     KYIFFEQTETESTLSLKNPITSPVVTQKKVPDNCCRREPMLVRNNQKCDSVEEETGIN
                     RERKVEVIKPLVAETDTPNRATFVVGNSSLLDTSSVLVTQEPEIELPREPRPNEECLQ
                     ILGNAEKGAKFLSDAEIIQLVNAKHIPAYKLETLMETHERGVSIRRQLLSKKLSEPSS
                     LQYLPYRDYNYSLVMGACCENVIGYMPIPVGVAGPLCLDEKEFQVPMATTEGCLVAST
                     NRGCRAIGLGGGASSRVLADGMTRGPVVRLPRACDSAEVKAWLETSEGFAVIKEAFDS
                     TSRFARLQKLHTSIAGRNLYIRFQSRSGDAMGMNMISKGTEKALSKLHEYFPEMQILA
                     VSGNYCTDKKPAAINWIEGRGKSVVCEAVIPAKVVREVLKTTTEAMIEVNINKNLVGS
                     AMAGSIGGYNAHAANIVTAIYIACGQDAAQNVGSSNCITLMEASGPTNEDLYISCTMP
                     SIEIGTVGGGTNLLPQQACLQMLGVQGACKDNPGENARQLARIVCGTVMAGELSLMAA
                     LAAGHLVKSHMIHNRSKINLQDLQGACTKKTA"
     exon            270..381
                     /gene="HMGCR"
                     /gene_synonym="LDLCQ3; LGMDR28; MYPLG"
                     /inference="alignment:Splign:2.1.0"
     exon            382..469
                     /gene="HMGCR"
                     /gene_synonym="LDLCQ3; LGMDR28; MYPLG"
                     /inference="alignment:Splign:2.1.0"
     exon            470..554
                     /gene="HMGCR"
                     /gene_synonym="LDLCQ3; LGMDR28; MYPLG"
                     /inference="alignment:Splign:2.1.0"
     exon            555..660
                     /gene="HMGCR"
                     /gene_synonym="LDLCQ3; LGMDR28; MYPLG"
                     /inference="alignment:Splign:2.1.0"
     exon            661..767
                     /gene="HMGCR"
                     /gene_synonym="LDLCQ3; LGMDR28; MYPLG"
                     /inference="alignment:Splign:2.1.0"
     exon            768..884
                     /gene="HMGCR"
                     /gene_synonym="LDLCQ3; LGMDR28; MYPLG"
                     /inference="alignment:Splign:2.1.0"
     exon            885..1045
                     /gene="HMGCR"
                     /gene_synonym="LDLCQ3; LGMDR28; MYPLG"
                     /inference="alignment:Splign:2.1.0"
     exon            1046..1293
                     /gene="HMGCR"
                     /gene_synonym="LDLCQ3; LGMDR28; MYPLG"
                     /inference="alignment:Splign:2.1.0"
     exon            1294..1472
                     /gene="HMGCR"
                     /gene_synonym="LDLCQ3; LGMDR28; MYPLG"
                     /inference="alignment:Splign:2.1.0"
     exon            1473..1667
                     /gene="HMGCR"
                     /gene_synonym="LDLCQ3; LGMDR28; MYPLG"
                     /inference="alignment:Splign:2.1.0"
     exon            1668..1826
                     /gene="HMGCR"
                     /gene_synonym="LDLCQ3; LGMDR28; MYPLG"
                     /inference="alignment:Splign:2.1.0"
     exon            1827..1984
                     /gene="HMGCR"
                     /gene_synonym="LDLCQ3; LGMDR28; MYPLG"
                     /inference="alignment:Splign:2.1.0"
     exon            1985..2090
                     /gene="HMGCR"
                     /gene_synonym="LDLCQ3; LGMDR28; MYPLG"
                     /inference="alignment:Splign:2.1.0"
     exon            2091..2261
                     /gene="HMGCR"
                     /gene_synonym="LDLCQ3; LGMDR28; MYPLG"
                     /inference="alignment:Splign:2.1.0"
     exon            2262..2402
                     /gene="HMGCR"
                     /gene_synonym="LDLCQ3; LGMDR28; MYPLG"
                     /inference="alignment:Splign:2.1.0"
     exon            2403..2561
                     /gene="HMGCR"
                     /gene_synonym="LDLCQ3; LGMDR28; MYPLG"
                     /inference="alignment:Splign:2.1.0"
     exon            2562..2716
                     /gene="HMGCR"
                     /gene_synonym="LDLCQ3; LGMDR28; MYPLG"
                     /inference="alignment:Splign:2.1.0"
     exon            2717..4530
                     /gene="HMGCR"
                     /gene_synonym="LDLCQ3; LGMDR28; MYPLG"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3485..3490
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HMGCR"
                     /gene_synonym="LDLCQ3; LGMDR28; MYPLG"
                     /note="hexamer: TATAAA"
     polyA_site      3504
                     /gene="HMGCR"
                     /gene_synonym="LDLCQ3; LGMDR28; MYPLG"
                     /note="major polyA site"
     regulatory      4131..4136
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HMGCR"
                     /gene_synonym="LDLCQ3; LGMDR28; MYPLG"
                     /note="hexamer: AATAAA"
     polyA_site      4158
                     /gene="HMGCR"
                     /gene_synonym="LDLCQ3; LGMDR28; MYPLG"
                     /note="major polyA site"
     regulatory      4508..4513
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HMGCR"
                     /gene_synonym="LDLCQ3; LGMDR28; MYPLG"
                     /note="hexamer: AATGAA"
     polyA_site      4530
                     /gene="HMGCR"
                     /gene_synonym="LDLCQ3; LGMDR28; MYPLG"
                     /note="major polyA site"
ORIGIN      
        1 ccttccgctc cgcgactgcg ttaactggag ccaggctgag cgtcggcgcc ggggttcggt
       61 ggcctctagt gagatctgga ggatccaagg attctgtagc tacaatgttg tcaagacttt
      121 ttcgaatgca tggcctcttt gtggcctccc atccctggga agtcatagtg gggacagtga
      181 cactgaccat ctgcatgatg tccatgaaca tgtttactgg taacaataag atctgtggtt
      241 ggaattatga atgtccaaag tttgaagagg atgttttgag cagtgacatt ataattctga
      301 caataacacg atgcatagcc atcctgtata tttacttcca gttccagaat ttacgtcaac
      361 ttggatcaaa atatattttg ggtattgctg gccttttcac aattttctca agttttgtat
      421 tcagtacagt tgtcattcac ttcttagaca aagaattgac aggcttgaat gaagctttgc
      481 cctttttcct acttttgatt gacctttcca gagcaagcac attagcaaag tttgccctca
      541 gttccaactc acaggatgaa gtaagggaaa atattgctcg tggaatggca attttaggtc
      601 ctacgtttac cctcgatgct cttgttgaat gtcttgtgat tggagttggt accatgtcag
      661 gggtacgtca gcttgaaatt atgtgctgct ttggctgcat gtcagttctt gccaactact
      721 tcgtgttcat gactttcttc ccagcttgtg tgtccttggt attagagctt tctcgggaaa
      781 gccgcgaggg tcgtccaatt tggcagctca gccattttgc ccgagtttta gaagaagaag
      841 aaaataagcc gaatcctgta actcagaggg tcaagatgat tatgtctcta ggcttggttc
      901 ttgttcatgc tcacagtcgc tggatagctg atccttctcc tcaaaacagt acagcagata
      961 cttctaaggt ttcattagga ctggatgaaa atgtgtccaa gagaattgaa ccaagtgttt
     1021 ccctctggca gttttatctc tctaaaatga tcagcatgga tattgaacaa gttattaccc
     1081 taagtttagc tctccttctg gctgtcaagt acatcttctt tgaacaaaca gagacagaat
     1141 ctacactctc attaaaaaac cctatcacat ctcctgtagt gacacaaaag aaagtcccag
     1201 acaattgttg tagacgtgaa cctatgctgg tcagaaataa ccagaaatgt gattcagtag
     1261 aggaagagac agggataaac cgagaaagaa aagttgaggt tataaaaccc ttagtggctg
     1321 aaacagatac cccaaacaga gctacatttg tggttggtaa ctcctcctta ctcgatactt
     1381 catcagtact ggtgacacag gaacctgaaa ttgaacttcc cagggaacct cggcctaatg
     1441 aagaatgtct acagatactt gggaatgcag agaaaggtgc aaaattcctt agtgatgctg
     1501 agatcatcca gttagtcaat gctaagcata tcccagccta caagttggaa actctgatgg
     1561 aaactcatga gcgtggtgta tctattcgcc gacagttact ttccaagaag ctttcagaac
     1621 cttcttctct ccagtaccta ccttacaggg attataatta ctccttggtg atgggagctt
     1681 gttgtgagaa tgttattgga tatatgccca tccctgttgg agtggcagga cccctttgct
     1741 tagatgaaaa agaatttcag gttccaatgg caacaacaga aggttgtctt gtggccagca
     1801 ccaatagagg ctgcagagca ataggtcttg gtggaggtgc cagcagccga gtccttgcag
     1861 atgggatgac tcgtggccca gttgtgcgtc ttccacgtgc ttgtgactct gcagaagtga
     1921 aagcctggct cgaaacatct gaagggttcg cagtgataaa ggaggcattt gacagcacta
     1981 gcagatttgc acgtctacag aaacttcata caagtatagc tggacgcaac ctttatatcc
     2041 gtttccagtc caggtcaggg gatgccatgg ggatgaacat gatttcaaag ggtacagaga
     2101 aagcactttc aaaacttcac gagtatttcc ctgaaatgca gattctagcc gttagtggta
     2161 actattgtac tgacaagaaa cctgctgcta taaattggat agagggaaga ggaaaatctg
     2221 ttgtttgtga agctgtcatt ccagccaagg ttgtcagaga agtattaaag actaccacag
     2281 aggctatgat tgaggtcaac attaacaaga atttagtggg ctctgccatg gctgggagca
     2341 taggaggcta caacgcccat gcagcaaaca ttgtcaccgc catctacatt gcctgtggac
     2401 aggatgcagc acagaatgtt ggtagttcaa actgtattac tttaatggaa gcaagtggtc
     2461 ccacaaatga agatttatat atcagctgca ccatgccatc tatagagata ggaacggtgg
     2521 gtggtgggac caacctacta cctcagcaag cctgtttgca gatgctaggt gttcaaggag
     2581 catgcaaaga taatcctggg gaaaatgccc ggcagcttgc ccgaattgtg tgtgggaccg
     2641 taatggctgg ggaattgtca cttatggcag cattggcagc aggacatctt gtcaaaagtc
     2701 acatgattca caacaggtcg aagatcaatt tacaagacct ccaaggagct tgcaccaaga
     2761 agacagcctg aatagcccga cagttctgaa ctggaacatg ggcattgggt tctaaaggac
     2821 taacataaaa tctgtgaatt aaaaaagctc aatgcattgt cttgtggagg atgaatagat
     2881 gtgatcactg agacagccac ttggtttttg gctctttcag agaggtctca ggttctttcc
     2941 atgcagactc ctcagatctg aacacagttt agtgctttac atgctgtgct ctttgaagag
     3001 atttcaacaa gaatattgta tgttaaagca tcagagatgg taatctacag ctcacctctg
     3061 aaggcaaata taagctggga aaaaagtttt gatgaaattc ttgaagttca tggtgatcag
     3121 tgcaattgac cttctccctc actcctgcca gttgaaaatg gatttttaaa ttatactgta
     3181 gctgatgaaa ctcctgattt tgtagttaat ttattaagtc tgggatgtag aacttcaaga
     3241 agtaagagct aagttctaag ttcatgtttg taaattaata cttcatttgg tgctggtcta
     3301 ttttgatttt ggggggtaat cagcattatt cttcagaagg ggacctgttt tcttcaaggg
     3361 aagaaacact cttattccca aactacagaa taatgtgtta aacatgctaa atagttctat
     3421 caggaaaaca aatcactgta tttatctccg caggctattt gttcagagag gccttttgtt
     3481 taaatataaa tgtttaaata taaatgtttg tctggattgg ctataacatg tctttcagca
     3541 ttaggctttt aagaaacaca gggttttgta ttctttacta aagatatcag agctcttaat
     3601 gttgcttaga tgagggtgac tgtcaagtac aagcaagact gggaccttag aaatcattgt
     3661 agaaacacag ttttgaaaga aaaataccat gtctctaagc caactttaat tgcttaaaag
     3721 acatttttat ttagttgaaa aatctagttt tttttgtaaa ctgtatcaaa tctgtatatg
     3781 ttgtaataaa acttatgcta gtttattgga agtgttcaag aaataaaaat caacttgtgt
     3841 actgataaaa tactctagcc tgggccagag aagataatgt tctttaatgt tgtccaggaa
     3901 accctggctt gcttgccgag cctaatgaaa gggaaagtca gctttcagag ccagtgaagg
     3961 agccacgtga atggccctag aactgtgcct agttcctgtg gccaggaggt tggtgactga
     4021 aacattcaca cagggctctt tgatggaccc acgaacgctc ttagctttct cagggggtca
     4081 gcagagttat tgaatcttaa ttttttttaa tgtacaagtt ttgtataaat aataaagaac
     4141 tccttatttt gtattacatc taatgcttca agtgttgctc ttggaaagct gatgatgtct
     4201 cttgtagaag atggactctg aaaaacattc caggaaacca tggcagcatg gagagcctct
     4261 tagtgattgt gtctgcattg ttattgtgga agatttacct tttctgttgt acgtaaagct
     4321 taaattgctt ttgttgtgac tttttagcca gtgacttttt ctgagctttt catggaagtg
     4381 gcagtgaaaa atatgttgag tgttcatttt agtgactgta attaatatct tgctggatta
     4441 atgttttgta caattactaa attgtataca ttttgttata gaatactttt ttctagtttc
     4501 agtaaataat gaaaaggaag ttaataccaa
//
