LOCUS       NM_005516               2548 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens major histocompatibility complex, class I, E (HLA-E),
            mRNA.
ACCESSION   NM_005516
VERSION     NM_005516.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Jost,S., Lucar,O., Lee,E., Yoder,T., Kroll,K., Sugawara,S.,
            Smith,S., Jones,R., Tweet,G., Werner,A., Tomezsko,P.J., Dugan,H.L.,
            Ghofrani,J., Rascle,P., Altfeld,M., Muller-Trutwin,M., Goepfert,P.
            and Reeves,R.K.
  TITLE     Antigen-specific memory NK cell responses against HIV and influenza
            use the NKG2/HLA-E axis
  JOURNAL   Sci Immunol 8 (90), eadi3974 (2023)
   PUBMED   38064568
  REMARK    GeneRIF: Antigen-specific memory NK cell responses against HIV and
            influenza use the NKG2/HLA-E axis.
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Zheng,H., Guan,X., Meng,X., Tong,Y., Wang,Y., Xie,S., Guo,L. and
            Lu,R.
  TITLE     IFN-gamma in ovarian tumor microenvironment upregulates HLA-E
            expression and predicts a poor prognosis
  JOURNAL   J Ovarian Res 16 (1), 229 (2023)
   PUBMED   38007483
  REMARK    GeneRIF: IFN-gamma in ovarian tumor microenvironment upregulates
            HLA-E expression and predicts a poor prognosis.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2548)
  AUTHORS   Siemaszko,J., Lacina,P., Szymczak,D., Szeremet,A., Majcherek,M.,
            Czyz,A., Sobczyk-Kruszelnicka,M., Fidyk,W., Solarska,I.,
            Nasilowska-Adamska,B., Skowronska,P., Bieniaszewska,M.,
            Tomaszewska,A., Basak,G.W., Giebel,S., Wrobel,T. and
            Bogunia-Kubik,K.
  TITLE     Significance of HLA-E and its two NKG2 receptors in development of
            complications after allogeneic transplantation of hematopoietic
            stem cells
  JOURNAL   Front Immunol 14, 1227897 (2023)
   PUBMED   37901227
  REMARK    GeneRIF: Significance of HLA-E and its two NKG2 receptors in
            development of complications after allogeneic transplantation of
            hematopoietic stem cells.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2548)
  AUTHORS   Castano-Nunez,A.L., Montes-Cano,M.A., Garcia-Lozano,J.R.,
            Ortego-Centeno,N., Garcia-Hernandez,F.J., Espinosa,G.,
            Grana-Gil,G., Sanchez-Burson,J., Julia,M.R., Solans,R., Blanco,R.,
            Barnosi-Marin,A.C., Gomez de la Torre,R., Fanlo,P.,
            Rodriguez-Carballeira,M., Rodriguez-Rodriguez,L., Camps,T.,
            Castaneda,S., Alegre-Sancho,J.J., Martin,J. and
            Gonzalez-Escribano,M.F.
  TITLE     The complex HLA-E-nonapeptide in Behcet disease
  JOURNAL   Front Immunol 14, 1080047 (2023)
   PUBMED   37638008
  REMARK    GeneRIF: The complex HLA-E-nonapeptide in Behcet disease.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2548)
  AUTHORS   Huisman,B.D., Guan,N., Ruckert,T., Garner,L., Singh,N.K.,
            McMichael,A.J., Gillespie,G.M., Romagnani,C. and Birnbaum,M.E.
  TITLE     High-throughput characterization of HLA-E-presented CD94/NKG2x
            ligands reveals peptides which modulate NK cell activation
  JOURNAL   Nat Commun 14 (1), 4809 (2023)
   PUBMED   37558657
  REMARK    GeneRIF: High-throughput characterization of HLA-E-presented
            CD94/NKG2x ligands reveals peptides which modulate NK cell
            activation.
            Erratum:[Nat Commun. 2023 Aug 29;14(1):5264. doi:
            10.1038/s41467-023-40946-y.. PMID: 37644057]
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2548)
  AUTHORS   Ulbrecht,M., Honka,T., Person,S., Johnson,J.P. and Weiss,E.H.
  TITLE     The HLA-E gene encodes two differentially regulated transcripts and
            a cell surface protein
  JOURNAL   J Immunol 149 (9), 2945-2953 (1992)
   PUBMED   1401923
REFERENCE   7  (bases 1 to 2548)
  AUTHORS   Houlihan,J.M., Biro,P.A., Fergar-Payne,A., Simpson,K.L. and
            Holmes,C.H.
  TITLE     Evidence for the expression of non-HLA-A,-B,-C class I genes in the
            human fetal liver
  JOURNAL   J Immunol 149 (2), 668-675 (1992)
   PUBMED   1624808
REFERENCE   8  (bases 1 to 2548)
  AUTHORS   Truong,M.J., Gruart,V., Capron,A., Capron,M. and Tourvieille,B.
  TITLE     Cloning and expression of a cDNA encoding a non-classical MHC class
            I antigen (HLA-E) in eosinophils from hypereosinophilic patients
  JOURNAL   J Immunol 148 (2), 627-632 (1992)
   PUBMED   1530866
REFERENCE   9  (bases 1 to 2548)
  AUTHORS   Wei,X.H. and Orr,H.T.
  TITLE     Differential expression of HLA-E, HLA-F, and HLA-G transcripts in
            human tissue
  JOURNAL   Hum Immunol 29 (2), 131-142 (1990)
   PUBMED   2249951
REFERENCE   10 (bases 1 to 2548)
  AUTHORS   Ohya,K., Kondo,K. and Mizuno,S.
  TITLE     Polymorphism in the human class I MHC locus HLA-E in Japanese
  JOURNAL   Immunogenetics 32 (3), 205-209 (1990)
   PUBMED   1977695
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK292391.1, BC002578.2,
            X56841.1 and CA439575.1.
            
            On Nov 22, 2018 this sequence version replaced NM_005516.5.
            
            Summary: HLA-E belongs to the HLA class I heavy chain paralogues.
            This class I molecule is a heterodimer consisting of a heavy chain
            and a light chain (beta-2 microglobulin). The heavy chain is
            anchored in the membrane. HLA-E binds a restricted subset of
            peptides derived from the leader peptides of other class I
            molecules. The heavy chain is approximately 45 kDa and its gene
            contains 8 exons. Exon one encodes the leader peptide, exons 2 and
            3 encode the alpha1 and alpha2 domains, which both bind the
            peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the
            transmembrane region, and exons 6 and 7 encode the cytoplasmic
            tail. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660803.96282.1,
                                           SRR1803611.256956.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000376630.5/ ENSP00000365817.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-488               AK292391.1         56-543
            489-1661            BC002578.2         480-1652
            1662-2214           X56841.1           925-1477
            2215-2548           CA439575.1         29-362              c
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6p22.1"
     gene            1..2548
                     /gene="HLA-E"
                     /gene_synonym="HLA-6.2; QA1"
                     /note="major histocompatibility complex, class I, E"
                     /db_xref="GeneID:3133"
                     /db_xref="HGNC:HGNC:4962"
                     /db_xref="MIM:143010"
     exon            1..87
                     /gene="HLA-E"
                     /gene_synonym="HLA-6.2; QA1"
                     /inference="alignment:Splign:2.1.0"
     CDS             24..1100
                     /gene="HLA-E"
                     /gene_synonym="HLA-6.2; QA1"
                     /note="HLA class I histocompatibility antigen, alpha chain
                     E; MHC class I antigen E; MHC class Ib antigen;
                     non-classical MHC class I antigen; nonclassical MHC class
                     I antigen"
                     /codon_start=1
                     /product="HLA class I histocompatibility antigen, alpha
                     chain E precursor"
                     /protein_id="NP_005507.3"
                     /db_xref="CCDS:CCDS34379.1"
                     /db_xref="GeneID:3133"
                     /db_xref="HGNC:HGNC:4962"
                     /db_xref="MIM:143010"
                     /translation="MVDGTLLLLLSEALALTQTWAGSHSLKYFHTSVSRPGRGEPRFI
                     SVGYVDDTQFVRFDNDAASPRMVPRAPWMEQEGSEYWDRETRSARDTAQIFRVNLRTL
                     RGYYNQSEAGSHTLQWMHGCELGPDGRFLRGYEQFAYDGKDYLTLNEDLRSWTAVDTA
                     AQISEQKSNDASEAEHQRAYLEDTCVEWLHKYLEKGKETLLHLEPPKTHVTHHPISDH
                     EATLRCWALGFYPAEITLTWQQDGEGHTQDTELVETRPAGDGTFQKWAAVVVPSGEEQ
                     RYTCHVQHEGLPEPVTLRWKPASQPTIPIVGIIAGLVLLGSVVSGAVVAAVIWRKKSS
                     GGKGGSYSKAEWSDSAQGSESHSL"
     exon            88..357
                     /gene="HLA-E"
                     /gene_synonym="HLA-6.2; QA1"
                     /inference="alignment:Splign:2.1.0"
     exon            358..633
                     /gene="HLA-E"
                     /gene_synonym="HLA-6.2; QA1"
                     /inference="alignment:Splign:2.1.0"
     exon            634..909
                     /gene="HLA-E"
                     /gene_synonym="HLA-6.2; QA1"
                     /inference="alignment:Splign:2.1.0"
     exon            910..1026
                     /gene="HLA-E"
                     /gene_synonym="HLA-6.2; QA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1027..1059
                     /gene="HLA-E"
                     /gene_synonym="HLA-6.2; QA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1060..1102
                     /gene="HLA-E"
                     /gene_synonym="HLA-6.2; QA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1103..2548
                     /gene="HLA-E"
                     /gene_synonym="HLA-6.2; QA1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2525..2530
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HLA-E"
                     /gene_synonym="HLA-6.2; QA1"
                     /note="hexamer: AATAAA"
     polyA_site      2548
                     /gene="HLA-E"
                     /gene_synonym="HLA-6.2; QA1"
                     /note="major polyA site"
ORIGIN      
        1 ctcaggactc agaggctggg atcatggtag atggaaccct ccttttactc ctctcggagg
       61 ccctggccct tacccagacc tgggcgggct cccactcctt gaagtatttc cacacttccg
      121 tgtcccggcc cggccgcggg gagccccgct tcatctctgt gggctacgtg gacgacaccc
      181 agttcgtgcg cttcgacaac gacgccgcga gtccgaggat ggtgccgcgg gcgccgtgga
      241 tggagcagga ggggtcagag tattgggacc gggagacacg gagcgccagg gacaccgcac
      301 agattttccg agtgaatctg cggacgctgc gcggctacta caatcagagc gaggccgggt
      361 ctcacaccct gcagtggatg catggctgcg agctggggcc cgacgggcgc ttcctccgcg
      421 ggtatgaaca gttcgcctac gacggcaagg attatctcac cctgaatgag gacctgcgct
      481 cctggaccgc ggtggacacg gcggctcaga tctccgagca aaagtcaaat gatgcctctg
      541 aggcggagca ccagagagcc tacctggaag acacatgcgt ggagtggctc cacaaatacc
      601 tggagaaggg gaaggagacg ctgcttcacc tggagccccc aaagacacac gtgactcacc
      661 accccatctc tgaccatgag gccaccctga ggtgctgggc cctgggcttc taccctgcgg
      721 agatcacact gacctggcag caggatgggg agggccatac ccaggacacg gagctcgtgg
      781 agaccaggcc tgcaggggat ggaaccttcc agaagtgggc agctgtggtg gtgccttctg
      841 gagaggagca gagatacacg tgccatgtgc agcatgaggg gctacccgag cccgtcaccc
      901 tgagatggaa gccggcttcc cagcccacca tccccatcgt gggcatcatt gctggcctgg
      961 ttctccttgg atctgtggtc tctggagctg tggttgctgc tgtgatatgg aggaagaaga
     1021 gctcaggtgg aaaaggaggg agctactcta aggctgagtg gagcgacagt gcccaggggt
     1081 ctgagtctca cagcttgtaa agcctgagac agctgccttg tgtgcgactg agatgcacag
     1141 ctgccttgtg tgcgactgag atgcaggatt tcctcacgcc tcccctatgt gtcttagggg
     1201 actctggctt ctctttttgc aagggcctct gaatctgtct gtgtccctgt tagcacaatg
     1261 tgaggaggta gagaaacagt ccacctctgt gtctaccatg acccccttcc tcacactgac
     1321 ctgtgttcct tccctgttct cttttctatt aaaaataaga acctgggcag agtgcggcag
     1381 ctcatgcctg taatcccagc acttagggag gccgaggagg gcagatcacg aggtcaggag
     1441 atcgaaacca tcctggctaa cacggtgaaa ccccgtctct actaaaaaat acaaaaaatt
     1501 agctgggcgc agaggcacgg gcctgtagtc ccagctactc aggaggcgga ggcaggagaa
     1561 tggcgtcaac ccgggaggcg gaggttgcag tgagccagga ttgtgcgact gcactccagc
     1621 ctgggtgaca gggtgaaacg ccatctcaaa aaataaaaat tgaaaaataa aaaaagaacc
     1681 tggatctcaa tttaattttt catattcttg caatgaaatg gacttgagga agctaagatc
     1741 atagctagaa atacagataa ttccacagca catctctagc aaatttagcc tattcctatt
     1801 ctctagccta ttccttacca cctgtaatct tgaccatata ccttggagtt gaatattgtt
     1861 ttcatactgc tgtggtttga atgttccctc caacactcat gttgagactt aatccctaat
     1921 gtggcaatac tgaaaggtgg ggcctttgag atgtgattgg atcgtaaggc tgtgccttca
     1981 ttcatgggtt aatggattaa tgggttatca caggaatggg actggtggct ttataagaag
     2041 aggaaaagag aactgagcta gcatgcccag cccacagaga gcctccacta gagtgatgct
     2101 aagtggaaat gtgaggtgca gctgccacag agggccccca ccagggaaat gtctagtgtc
     2161 tagtggatcc aggccacagg agagagtgcc ttgtggagcg ctgggagcag gacctgacca
     2221 ccaccaggac cccagaactg tggagtcagt ggcagcatgc agcgccccct tgggaaagct
     2281 ttaggcacca gcctgcaacc cattcgagca gccacgtagg ctgcacccag caaagccaca
     2341 ggcacggggc tacctgaggc cttgggggcc caatccctgc tccagtgtgt ccgtgaggca
     2401 gcacacgaag tcaaaagaga ttattctctt cccacagata ccttttctct cccatgaccc
     2461 tttaacagca tctgcttcat tcccctcacc ttcccaggct gatctgaggt aaactttgaa
     2521 gtaaaataaa agctgtgttt gagcatca
//
