LOCUS       NM_002115               3082 bp    mRNA    linear   PRI 06-AUG-2024
DEFINITION  Homo sapiens hexokinase 3 (HK3), mRNA.
ACCESSION   NM_002115
VERSION     NM_002115.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Liang,T., Zhou,X., Wang,Y. and Ma,W.
  TITLE     Glioma hexokinase 3 positively correlates with malignancy and
            macrophage infiltration
  JOURNAL   Metab Brain Dis 39 (5), 719-729 (2024)
   PUBMED   38687460
  REMARK    GeneRIF: Glioma hexokinase 3 positively correlates with malignancy
            and macrophage infiltration.
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Seiler,K., Humbert,M., Minder,P., Mashimo,I., Schlafli,A.M.,
            Krauer,D., Federzoni,E.A., Vu,B., Moresco,J.J., Yates,J.R. 3rd,
            Sadowski,M.C., Radpour,R., Kaufmann,T., Sarry,J.E., Dengjel,J.,
            Tschan,M.P. and Torbett,B.E.
  TITLE     Hexokinase 3 enhances myeloid cell survival via non-glycolytic
            functions
  JOURNAL   Cell Death Dis 13 (5), 448 (2022)
   PUBMED   35538058
  REMARK    GeneRIF: Hexokinase 3 enhances myeloid cell survival via
            non-glycolytic functions.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3082)
  AUTHORS   Xu,W., Liu,W.R., Xu,Y., Tian,X., Anwaier,A., Su,J.Q., Zhu,W.K.,
            Shi,G.H., Wei,G.M., Huang,Y.P., Qu,Y.Y., Zhang,H.L. and Ye,D.W.
  TITLE     Hexokinase 3 dysfunction promotes tumorigenesis and immune escape
            by upregulating monocyte/macrophage infiltration into the clear
            cell renal cell carcinoma microenvironment
  JOURNAL   Int J Biol Sci 17 (9), 2205-2222 (2021)
   PUBMED   34239350
  REMARK    GeneRIF: Hexokinase 3 dysfunction promotes tumorigenesis and immune
            escape by upregulating monocyte/macrophage infiltration into the
            clear cell renal cell carcinoma microenvironment.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3082)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   5  (bases 1 to 3082)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 3082)
  AUTHORS   Furuta,H., Nishi,S., Le Beau,M.M., Fernald,A.A., Yano,H. and
            Bell,G.I.
  TITLE     Sequence of human hexokinase III cDNA and assignment of the human
            hexokinase III gene (HK3) to chromosome band 5q35.2 by fluorescence
            in situ hybridization
  JOURNAL   Genomics 36 (1), 206-209 (1996)
   PUBMED   8812439
REFERENCE   7  (bases 1 to 3082)
  AUTHORS   Palma,F., Agostini,D., Mason,P., Dacha,M., Piccoli,G.,
            Biagiarelli,B., Fiorani,M. and Stocchi,V.
  TITLE     Purification and characterization of the carboxyl-domain of human
            hexokinase type III expressed as fusion protein
  JOURNAL   Mol Cell Biochem 155 (1), 23-29 (1996)
   PUBMED   8717435
REFERENCE   8  (bases 1 to 3082)
  AUTHORS   Reid,S. and Masters,C.
  TITLE     On the developmental properties and tissue interactions of
            hexokinase
  JOURNAL   Mech Ageing Dev 31 (2), 197-212 (1985)
   PUBMED   4058069
REFERENCE   9  (bases 1 to 3082)
  AUTHORS   Rijksen,G., Staal,G.E., Beks,P.J., Streefkerk,M. and Akkerman,J.W.
  TITLE     Compartmentation of hexokinase in human blood cells.
            Characterization of soluble and particulate enzymes
  JOURNAL   Biochim Biophys Acta 719 (3), 431-437 (1982)
   PUBMED   7150652
REFERENCE   10 (bases 1 to 3082)
  AUTHORS   Povey,S., Corney,G. and Harris,H.
  TITLE     Genetically determined polymorphism of a form of hexokinase, HK
            III, found in human leucocytes
  JOURNAL   Ann Hum Genet 38 (4), 407-415 (1975)
   PUBMED   1190733
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC027318.5, BC028129.1,
            BX644742.1 and U51333.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_002115.2.
            
            Summary: Hexokinases phosphorylate glucose to produce
            glucose-6-phosphate, the first step in most glucose metabolism
            pathways. This gene encodes hexokinase 3. Similar to hexokinases 1
            and 2, this allosteric enzyme is inhibited by its product
            glucose-6-phosphate. [provided by RefSeq, Apr 2009].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U51333.1, SRR14038193.2035666.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            MANE Ensembl match                 :: ENST00000292432.10/
                                                  ENSP00000292432.5
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-14                AC027318.5         42108-42121         c
            15-1518             BC028129.1         1-1504
            1519-1811           BX644742.1         444-736
            1812-3080           BC028129.1         1798-3066
            3081-3082           U51333.1           3049-3050
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q35.2"
     gene            1..3082
                     /gene="HK3"
                     /gene_synonym="HKIII; HXK3"
                     /note="hexokinase 3"
                     /db_xref="GeneID:3101"
                     /db_xref="HGNC:HGNC:4925"
                     /db_xref="MIM:142570"
     exon            1..80
                     /gene="HK3"
                     /gene_synonym="HKIII; HXK3"
                     /inference="alignment:Splign:2.1.0"
     exon            81..202
                     /gene="HK3"
                     /gene_synonym="HKIII; HXK3"
                     /inference="alignment:Splign:2.1.0"
     CDS             107..2878
                     /gene="HK3"
                     /gene_synonym="HKIII; HXK3"
                     /EC_number="2.7.1.1"
                     /note="ATP:D-hexose 6-phosphotransferase; HK III;
                     hexokinase 3 (white cell); hexokinase type III;
                     hexokinase-C"
                     /codon_start=1
                     /product="hexokinase-3"
                     /protein_id="NP_002106.2"
                     /db_xref="CCDS:CCDS4407.1"
                     /db_xref="GeneID:3101"
                     /db_xref="HGNC:HGNC:4925"
                     /db_xref="MIM:142570"
                     /translation="MDSIGSSGLRQGEETLSCSEEGLPGPSDSSELVQECLQQFKVTR
                     AQLQQIQASLLGSMEQALRGQASPAPAVRMLPTYVGSTPHGTEQGDFVVLELGATGAS
                     LRVLWVTLTGIEGHRVEPRSQEFVIPQEVMLGAGQQLFDFAAHCLSEFLDAQPVNKQG
                     LQLGFSFSFPCHQTGLDRSTLISWTKGFRCSGVEGQDVVQLLRDAIRRQGAYNIDVVA
                     VVNDTVGTMMGCEPGVRPCEVGLVVDTGTNACYMEEARHVAVLDEDRGRVCVSVEWGS
                     FSDDGALGPVLTTFDHTLDHESLNPGAQRFEKMIGGLYLGELVRLVLAHLARCGVLFG
                     GCTSPALLSQGSILLEHVAEMEDPSTGAARVHAILQDLGLSPGASDVELVQHVCAAVC
                     TRAAQLCAAALAAVLSCLQHSREQQTLQVAVATGGRVCERHPRFCSVLQGTVMLLAPE
                     CDVSLIPSVDGGGRGVAMVTAVAARLAAHRRLLEETLAPFRLNHDQLAAVQAQMRKAM
                     AKGLRGEASSLRMLPTFVRATPDGSERGDFLALDLGGTNFRVLLVRVTTGVQITSEIY
                     SIPETVAQGSGQQLFDHIVDCIVDFQQKQGLSGQSLPLGFTFSFPCRQLGLDQGILLN
                     WTKGFKASDCEGQDVVSLLREAITRRQAVELNVVAIVNDTVGTMMSCGYEDPRCEIGL
                     IVGTGTNACYMEELRNVAGVPGDSGRMCINMEWGAFGDDGSLAMLSTRFDASVDQASI
                     NPGKQRFEKMISGMYLGEIVRHILLHLTSLGVLFRGQQIQRLQTRDIFKTKFLSEIES
                     DSLALRQVRAILEDLGLPLTSDDALMVLEVCQAVSQRAAQLCGAGVAAVVEKIRENRG
                     LEELAVSVGVDGTLYKLHPRFSSLVAATVRELAPRCVVTFLQSEDGSGKGAALVTAVA
                     CRLAQLTRV"
     exon            203..365
                     /gene="HK3"
                     /gene_synonym="HKIII; HXK3"
                     /inference="alignment:Splign:2.1.0"
     exon            366..520
                     /gene="HK3"
                     /gene_synonym="HKIII; HXK3"
                     /inference="alignment:Splign:2.1.0"
     exon            521..640
                     /gene="HK3"
                     /gene_synonym="HKIII; HXK3"
                     /inference="alignment:Splign:2.1.0"
     exon            641..736
                     /gene="HK3"
                     /gene_synonym="HKIII; HXK3"
                     /inference="alignment:Splign:2.1.0"
     exon            737..836
                     /gene="HK3"
                     /gene_synonym="HKIII; HXK3"
                     /inference="alignment:Splign:2.1.0"
     exon            837..1020
                     /gene="HK3"
                     /gene_synonym="HKIII; HXK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1021..1176
                     /gene="HK3"
                     /gene_synonym="HKIII; HXK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1177..1410
                     /gene="HK3"
                     /gene_synonym="HKIII; HXK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1411..1706
                     /gene="HK3"
                     /gene_synonym="HKIII; HXK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1707..1843
                     /gene="HK3"
                     /gene_synonym="HKIII; HXK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1844..1963
                     /gene="HK3"
                     /gene_synonym="HKIII; HXK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1964..2059
                     /gene="HK3"
                     /gene_synonym="HKIII; HXK3"
                     /inference="alignment:Splign:2.1.0"
     exon            2060..2159
                     /gene="HK3"
                     /gene_synonym="HKIII; HXK3"
                     /inference="alignment:Splign:2.1.0"
     exon            2160..2343
                     /gene="HK3"
                     /gene_synonym="HKIII; HXK3"
                     /inference="alignment:Splign:2.1.0"
     exon            2344..2499
                     /gene="HK3"
                     /gene_synonym="HKIII; HXK3"
                     /inference="alignment:Splign:2.1.0"
     exon            2500..2733
                     /gene="HK3"
                     /gene_synonym="HKIII; HXK3"
                     /inference="alignment:Splign:2.1.0"
     exon            2734..3082
                     /gene="HK3"
                     /gene_synonym="HKIII; HXK3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3058..3063
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HK3"
                     /gene_synonym="HKIII; HXK3"
                     /note="hexamer: AATATA"
     polyA_site      3082
                     /gene="HK3"
                     /gene_synonym="HKIII; HXK3"
                     /note="major polyA site"
ORIGIN      
        1 gcttttggtc attggggtgc ctcatattgc cagacaagag ctcagacctg aggagagtga
       61 ctagcttctc tgtgtcccag gtggccacct tccactgtgg aagctcatgg actccattgg
      121 gtcttcaggg ttgcggcagg gggaagaaac cctgagttgc tctgaggagg gcttgcccgg
      181 gccctcagac agctcagagc tggtgcagga gtgcctgcag cagttcaagg tgacaagggc
      241 acagctacag cagatccaag ccagcctctt gggttccatg gagcaggcgc tgaggggaca
      301 ggccagccct gcccctgcgg tccggatgct gcctacatac gtggggtcca ccccacatgg
      361 cactgagcaa ggagacttcg tggtgctgga gctgggggcc acaggggcct cactgcgtgt
      421 tttgtgggtg actctaactg gcattgaggg gcatagggtg gagcccagaa gccaggagtt
      481 tgtgatcccc caagaggtga tgctgggtgc tggccagcag ctctttgact ttgctgccca
      541 ctgcctgtct gagttcctgg atgcgcagcc tgtgaacaaa cagggtctgc agcttggctt
      601 cagcttctct ttcccttgtc accagacggg cttggacagg agcaccctca tttcctggac
      661 caaaggtttt aggtgcagtg gtgtggaagg ccaggatgtg gtccagctgc tgagagatgc
      721 cattcggagg cagggggcct acaacatcga cgtggttgct gtggtgaacg acacagtggg
      781 caccatgatg ggctgtgagc cgggggtcag gccgtgtgag gttgggctag ttgtagacac
      841 gggcaccaac gcgtgttaca tggaggaggc acggcatgtg gcagtgctgg acgaagaccg
      901 gggccgcgtc tgcgtcagcg tcgagtgggg ctccttcagc gatgatgggg cgctgggacc
      961 agtgctgacc accttcgacc ataccctgga ccatgagtcc ctgaatcctg gtgctcagag
     1021 gtttgagaag atgatcggag gcctgtacct gggtgagctg gtgcggctgg tgctggctca
     1081 cttggcccgg tgtggggtcc tctttggtgg ctgcacctcc cctgccctgc tgagccaagg
     1141 cagcatcctc ctggaacacg tggctgagat ggaggacccc tctactgggg cagcccgtgt
     1201 ccatgctatc ctgcaggact tgggcctgag ccctggggct tcggatgttg agcttgtgca
     1261 gcacgtctgt gcggccgtgt gcacgcgggc tgcccagctc tgtgctgccg ccctggccgc
     1321 tgttctctcc tgcctccagc acagccggga gcaacaaaca ctccaggttg ctgtggccac
     1381 cggaggccga gtgtgtgagc ggcaccccag gttctgcagc gtcctgcagg ggacagtgat
     1441 gctcctggcc ccggaatgcg atgtctcctt aatcccctct gtggatggtg gtggccgggg
     1501 agtggcgatg gtgactgccg tggctgcccg tctggctgcc caccggcgcc tgctggagga
     1561 gaccctggcc ccattccggt tgaaccatga tcaactggct gcggttcagg cacagatgcg
     1621 gaaggccatg gccaaggggc tccgagggga ggcctcctcc cttcgcatgc tgcccacttt
     1681 cgtccgggcc acccctgacg gcagcgagcg aggggatttc ctggccctgg acctcggggg
     1741 cacgaacttc cgtgtcctcc tggtacgtgt gaccacaggc gtgcagatca ccagcgagat
     1801 ctactccatt cccgagactg tggcccaggg ttctgggcag cagctctttg accacatcgt
     1861 ggactgcatc gtggacttcc agcagaagca gggcctgagc gggcagagcc tcccactggg
     1921 ttttaccttc tccttcccat gtaggcagct tggcctagac cagggcatcc tcctgaactg
     1981 gaccaagggt ttcaaggcat cagactgcga gggccaagat gtcgtgagtc tgttgcggga
     2041 agccatcact cgcagacagg cagtggagct gaatgtggtt gccattgtca atgacacggt
     2101 ggggaccatg atgtcctgtg gctatgagga cccccgttgc gagataggcc tcattgtcgg
     2161 aaccggcacc aatgcctgct acatggagga gctccggaat gtggcgggcg tgcctgggga
     2221 ctcaggccgc atgtgcatca acatggagtg gggcgccttt ggggacgatg gctctctggc
     2281 catgctcagc acccgctttg atgcaagtgt ggaccaggcg tccatcaacc ccggcaagca
     2341 gaggtttgaa aagatgatca gcggcatgta cctgggggag atcgtccgcc acatcctttt
     2401 acatttaacc agccttggcg ttctcttccg gggccagcag atccagcgcc ttcagaccag
     2461 ggacatcttc aagaccaagt tcctctctga gatcgaaagt gacagcctgg ccctgcggca
     2521 ggtccgagcc atcctagagg atctggggct acccctgacc tcagatgacg ccctgatggt
     2581 gctagaggtg tgccaggctg tgtcccagag ggctgcccag ctctgtgggg cgggtgtagc
     2641 tgccgtggtg gagaagatcc gggagaaccg gggcctggaa gagctggcag tgtctgtggg
     2701 ggtggatgga acgctctaca agctgcaccc gcgcttctcc agcctggtgg cggccacagt
     2761 gcgggagctg gcccctcgct gtgtggtcac gttcctgcag tcagaggatg ggtccggcaa
     2821 aggtgcggcc ctggtcaccg ctgttgcctg ccgccttgcg cagttgactc gtgtctgagg
     2881 aaacctccag gctgaggagg tctccgccgc agccttgctg gagccgggtc ggggtctgcc
     2941 tgtttcccag ccaggcccag ccacccagga ctcctgggac atcccatgtg tgacccctct
     3001 gcggccattt ggccttgctc cctggctttc cctgagagaa gtagcactca ggttagcaat
     3061 atatatatat aatttattta ca
//
