LOCUS       NM_000189               5626 bp    mRNA    linear   PRI 24-SEP-2024
DEFINITION  Homo sapiens hexokinase 2 (HK2), transcript variant 1, mRNA.
ACCESSION   NM_000189
VERSION     NM_000189.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5626)
  AUTHORS   Song,L., Zhao,X., Chen,J., Yin,H., Tang,H., Li,L., Dong,H., Li,X.,
            Qu,Z., Chu,X. and Guo,M.
  TITLE     RNA-binding protein SORBS2 increases human trophoblast cell
            migration via stabilizing HK2 mRNA in preeclampsia
  JOURNAL   Reproduction 168 (3), e240093 (2024)
   PUBMED   38995729
  REMARK    GeneRIF: RNA-binding protein SORBS2 increases human trophoblast
            cell migration via stabilizing HK2 mRNA in preeclampsia.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5626)
  AUTHORS   Tong,Y., Liu,X., Wu,L., Xiang,Y., Wang,J., Cheng,Y., Zhang,C.,
            Han,B., Wang,L. and Yan,D.
  TITLE     Hexokinase 2 nonmetabolic function-mediated phosphorylation of
            IkappaBalpha enhances pancreatic ductal adenocarcinoma progression
  JOURNAL   Cancer Sci 115 (8), 2673-2685 (2024)
   PUBMED   38801832
  REMARK    GeneRIF: Hexokinase 2 nonmetabolic function-mediated
            phosphorylation of IkappaBalpha enhances pancreatic ductal
            adenocarcinoma progression.
REFERENCE   3  (bases 1 to 5626)
  AUTHORS   Wang,L., Zhang,R.K., Sang,P., Xie,Y.X., Zhang,Y., Zhou,Z.H.,
            Wang,K.K., Zhou,F.M., Ji,X.B., Liu,W.J., Qiu,J.G. and Jiang,B.H.
  TITLE     HK2 and LDHA upregulation mediate hexavalent chromium-induced
            carcinogenesis, cancer development and prognosis through miR-218
            inhibition
  JOURNAL   Ecotoxicol Environ Saf 279, 116500 (2024)
   PUBMED   38795416
  REMARK    GeneRIF: HK2 and LDHA upregulation mediate hexavalent
            chromium-induced carcinogenesis, cancer development and prognosis
            through miR-218 inhibition.
REFERENCE   4  (bases 1 to 5626)
  AUTHORS   Yu,S.K., Yu,T., Wang,Y.M., Sun,A., Liu,J. and Lu,K.H.
  TITLE     CCT6A facilitates lung adenocarcinoma progression and glycolysis
            via STAT1/HK2 axis
  JOURNAL   J Transl Med 22 (1), 460 (2024)
   PUBMED   38750462
  REMARK    GeneRIF: CCT6A facilitates lung adenocarcinoma progression and
            glycolysis via STAT1/HK2 axis.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5626)
  AUTHORS   Yu,L.R., Han,X.Z., Tang,Y.Z., Liu,D., Luo,X.Q., Qiu,X.W., Feng,J.,
            Yuan,W.X. and Ding,J.Y.
  TITLE     Activation of the MEK/ERK Pathway Mediates the Inhibitory Effects
            of Silvestrol on Nasopharyngeal Carcinoma Cells via RAP1A, HK2, and
            GADD45A
  JOURNAL   Front Biosci (Landmark Ed) 29 (4), 160 (2024)
   PUBMED   38682208
  REMARK    GeneRIF: Activation of the MEK/ERK Pathway Mediates the Inhibitory
            Effects of Silvestrol on Nasopharyngeal Carcinoma Cells via RAP1A,
            HK2, and GADD45A.
REFERENCE   6  (bases 1 to 5626)
  AUTHORS   Mathupala,S.P., Rempel,A. and Pedersen,P.L.
  TITLE     Glucose catabolism in cancer cells. Isolation, sequence, and
            activity of the promoter for type II hexokinase
  JOURNAL   J Biol Chem 270 (28), 16918-16925 (1995)
   PUBMED   7622509
REFERENCE   7  (bases 1 to 5626)
  AUTHORS   Echwald,S.M., Bjorbaek,C., Hansen,T., Clausen,J.O., Vestergaard,H.,
            Zierath,J.R., Printz,R.L., Granner,D.K. and Pedersen,O.
  TITLE     Identification of four amino acid substitutions in hexokinase II
            and studies of relationships to NIDDM, glucose effectiveness, and
            insulin sensitivity
  JOURNAL   Diabetes 44 (3), 347-353 (1995)
   PUBMED   7883123
REFERENCE   8  (bases 1 to 5626)
  AUTHORS   Vidal-Puig,A., Printz,R.L., Stratton,I.M., Granner,D.K. and
            Moller,D.E.
  TITLE     Analysis of the hexokinase II gene in subjects with insulin
            resistance and NIDDM and detection of a Gln142-->His substitution
  JOURNAL   Diabetes 44 (3), 340-346 (1995)
   PUBMED   7883122
REFERENCE   9  (bases 1 to 5626)
  AUTHORS   Laakso,M., Malkki,M. and Deeb,S.S.
  TITLE     Amino acid substitutions in hexokinase II among patients with NIDDM
  JOURNAL   Diabetes 44 (3), 330-334 (1995)
   PUBMED   7883120
REFERENCE   10 (bases 1 to 5626)
  AUTHORS   Shinohara,Y., Yamamoto,K., Kogure,K., Ichihara,J. and Terada,H.
  TITLE     Steady state transcript levels of the type II hexokinase and type 1
            glucose transporter in human tumor cell lines
  JOURNAL   Cancer Lett 82 (1), 27-32 (1994)
   PUBMED   7518342
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC019069.8 and AC104135.5.
            
            On Jul 17, 2019 this sequence version replaced NM_000189.4.
            
            Summary: Hexokinases phosphorylate glucose to produce
            glucose-6-phosphate, the first step in most glucose metabolism
            pathways. This gene encodes hexokinase 2, the predominant form
            found in skeletal muscle. It localizes to the outer membrane of
            mitochondria. Expression of this gene is insulin-responsive, and
            studies in rat suggest that it is involved in the increased rate of
            glycolysis seen in rapidly growing cancer cells. [provided by
            RefSeq, Apr 2009].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC064369.1, SRR11853565.16550.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000290573.7/ ENSP00000290573.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-517               AC019069.8         23482-23998
            518-680             AC019069.8         43648-43810
            681-829             AC019069.8         56991-57139
            830-949             AC019069.8         61655-61774
            950-1045            AC019069.8         62631-62726
            1046-1145           AC019069.8         63199-63298
            1146-1329           AC019069.8         63621-63804
            1330-1393           AC019069.8         66521-66584
            1394-1485           AC104135.5         1-92
            1486-1719           AC104135.5         1459-1692
            1720-2024           AC104135.5         3036-3340
            2025-2173           AC104135.5         4482-4630
            2174-2293           AC104135.5         4891-5010
            2294-2389           AC104135.5         8265-8360
            2390-2489           AC104135.5         9065-9164
            2490-2673           AC104135.5         9261-9444
            2674-2829           AC104135.5         10674-10829
            2830-3063           AC104135.5         12016-12249
            3064-5626           AC104135.5         13568-16130
FEATURES             Location/Qualifiers
     source          1..5626
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p12"
     gene            1..5626
                     /gene="HK2"
                     /gene_synonym="HKII; HXK2"
                     /note="hexokinase 2"
                     /db_xref="GeneID:3099"
                     /db_xref="HGNC:HGNC:4923"
                     /db_xref="MIM:601125"
     exon            1..517
                     /gene="HK2"
                     /gene_synonym="HKII; HXK2"
                     /inference="alignment:Splign:2.1.0"
     CDS             455..3208
                     /gene="HK2"
                     /gene_synonym="HKII; HXK2"
                     /EC_number="2.7.1.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     hexokinase-2, muscle; hexokinase type II; muscle form
                     hexokinase; hexokinase-B"
                     /codon_start=1
                     /product="hexokinase-2 isoform 1"
                     /protein_id="NP_000180.2"
                     /db_xref="CCDS:CCDS1956.1"
                     /db_xref="GeneID:3099"
                     /db_xref="HGNC:HGNC:4923"
                     /db_xref="MIM:601125"
                     /translation="MIASHLLAYFFTELNHDQVQKVDQYLYHMRLSDETLLEISKRFR
                     KEMEKGLGATTHPTAAVKMLPTFVRSTPDGTEHGEFLALDLGGTNFRVLWVKVTDNGL
                     QKVEMENQIYAIPEDIMRGSGTQLFDHIAECLANFMDKLQIKDKKLPLGFTFSFPCHQ
                     TKLDESFLVSWTKGFKSSGVEGRDVVALIRKAIQRRGDFDIDIVAVVNDTVGTMMTCG
                     YDDHNCEIGLIVGTGSNACYMEEMRHIDMVEGDEGRMCINMEWGAFGDDGSLNDIRTE
                     FDQEIDMGSLNPGKQLFEKMISGMYMGELVRLILVKMAKEELLFGGKLSPELLNTGRF
                     ETKDISDIEGEKDGIRKAREVLMRLGLDPTQEDCVATHRICQIVSTRSASLCAATLAA
                     VLQRIKENKGEERLRSTIGVDGSVYKKHPHFAKRLHKTVRRLVPGCDVRFLRSEDGSG
                     KGAAMVTAVAYRLADQHRARQKTLEHLQLSHDQLLEVKRRMKVEMERGLSKETHASAP
                     VKMLPTYVCATPDGTEKGDFLALDLGGTNFRVLLVRVRNGKWGGVEMHNKIYAIPQEV
                     MHGTGDELFDHIVQCIADFLEYMGMKGVSLPLGFTFSFPCQQNSLDESILLKWTKGFK
                     ASGCEGEDVVTLLKEAIHRREEFDLDVVAVVNDTVGTMMTCGFEDPHCEVGLIVGTGS
                     NACYMEEMRNVELVEGEEGRMCVNMEWGAFGDNGCLDDFRTEFDVAVDELSLNPGKQR
                     FEKMISGMYLGEIVRNILIDFTKRGLLFRGRISERLKTRGIFETKFLSQIESDCLALL
                     QVRAILQHLGLESTCDDSIIVKEVCTVVARRAAQLCGAGMAAVVDRIRENRGLDALKV
                     TVGVDGTLYKLHPHFAKVMHETVKDLAPKCDVSFLQSEDGSGKGAALITAVACRIREA
                     GQR"
     exon            518..680
                     /gene="HK2"
                     /gene_synonym="HKII; HXK2"
                     /inference="alignment:Splign:2.1.0"
     exon            681..829
                     /gene="HK2"
                     /gene_synonym="HKII; HXK2"
                     /inference="alignment:Splign:2.1.0"
     exon            830..949
                     /gene="HK2"
                     /gene_synonym="HKII; HXK2"
                     /inference="alignment:Splign:2.1.0"
     exon            950..1045
                     /gene="HK2"
                     /gene_synonym="HKII; HXK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1046..1145
                     /gene="HK2"
                     /gene_synonym="HKII; HXK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1146..1329
                     /gene="HK2"
                     /gene_synonym="HKII; HXK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1330..1485
                     /gene="HK2"
                     /gene_synonym="HKII; HXK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1486..1719
                     /gene="HK2"
                     /gene_synonym="HKII; HXK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1720..2024
                     /gene="HK2"
                     /gene_synonym="HKII; HXK2"
                     /inference="alignment:Splign:2.1.0"
     exon            2025..2173
                     /gene="HK2"
                     /gene_synonym="HKII; HXK2"
                     /inference="alignment:Splign:2.1.0"
     exon            2174..2293
                     /gene="HK2"
                     /gene_synonym="HKII; HXK2"
                     /inference="alignment:Splign:2.1.0"
     exon            2294..2389
                     /gene="HK2"
                     /gene_synonym="HKII; HXK2"
                     /inference="alignment:Splign:2.1.0"
     exon            2390..2489
                     /gene="HK2"
                     /gene_synonym="HKII; HXK2"
                     /inference="alignment:Splign:2.1.0"
     exon            2490..2673
                     /gene="HK2"
                     /gene_synonym="HKII; HXK2"
                     /inference="alignment:Splign:2.1.0"
     exon            2674..2829
                     /gene="HK2"
                     /gene_synonym="HKII; HXK2"
                     /inference="alignment:Splign:2.1.0"
     exon            2830..3063
                     /gene="HK2"
                     /gene_synonym="HKII; HXK2"
                     /inference="alignment:Splign:2.1.0"
     exon            3064..5626
                     /gene="HK2"
                     /gene_synonym="HKII; HXK2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5385..5390
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HK2"
                     /gene_synonym="HKII; HXK2"
                     /note="hexamer: AATAAA"
     polyA_site      5414
                     /gene="HK2"
                     /gene_synonym="HKII; HXK2"
     regulatory      5595..5600
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HK2"
                     /gene_synonym="HKII; HXK2"
                     /note="hexamer: AATAAA"
     polyA_site      5626
                     /gene="HK2"
                     /gene_synonym="HKII; HXK2"
                     /note="major polyA site"
ORIGIN      
        1 acattgttgc atgaaactcc ggcgcaggag tcccgggctg ccgctggcaa catcgtgtca
       61 cccagctaag aaaatccgcg ggcccgagcc acgcgcctgt gaatcggaga ggtcccactg
      121 cccgagtgga gccgggctga gattcttctc aagttgagcc tcagtgatcc tgtggccgaa
      181 gttagcgcct tgacgtggga caaccggaca cgtcgccagg agagaactga ggcgccttct
      241 agcagttgtg acgccaaaat cacgtctccg gagacccgcg ccctccgcca gccgggcgca
      301 ccctcgccgg tagccttctt tgtgcgccgt ccggactccc agctcccggc ccggcagccg
      361 agccccagca caaagcagtc ggaccgcgcc gcccgcctcc cctctcgcgt ctccgcctcg
      421 gtttcccaac tctgcgccgt cgggccgcgg caggatgatt gcctcgcatc tgcttgccta
      481 cttcttcacg gagctcaacc atgaccaagt gcagaaggtt gaccagtatc tctaccacat
      541 gcgcctctct gatgagaccc tcttggagat ctctaagcgg ttccgcaagg agatggagaa
      601 agggcttgga gccaccactc accctactgc agcagtgaag atgctgccca cctttgtgag
      661 gtccactcca gatgggacag aacacggaga gttcctggct ctggatcttg gagggaccaa
      721 cttccgtgtg ctttgggtga aagtaacgga caatgggctc cagaaggtgg agatggagaa
      781 tcagatctat gccatccctg aggacatcat gcgaggcagt ggcacccagc tgtttgacca
      841 cattgccgaa tgcctggcta acttcatgga taagctacaa atcaaagaca agaagctccc
      901 actgggtttt accttctcgt tcccctgcca ccagactaaa ctagacgaga gtttcctggt
      961 ctcatggacc aagggattca agtccagtgg agtggaaggc agagacgttg tggctctgat
     1021 ccggaaggcc atccagagga gaggggactt tgatatcgac attgtggctg tggtgaatga
     1081 cacagttggg accatgatga cctgtggtta tgatgaccac aactgtgaga ttggtctcat
     1141 tgtgggcacg ggcagcaacg cctgctacat ggaagagatg cgccacatcg acatggtgga
     1201 aggcgatgag gggcggatgt gtatcaatat ggagtggggg gccttcgggg acgatggctc
     1261 gctcaacgac attcgcactg agtttgacca ggagattgac atgggctcac tgaacccggg
     1321 aaagcaactg tttgagaaga tgatcagtgg gatgtacatg ggggagctgg tgaggcttat
     1381 cctggtgaag atggccaagg aggagctgct ctttgggggg aagctcagcc cagagcttct
     1441 caacaccggt cgctttgaga ccaaagacat ctcagacatt gaaggggaga aggatggcat
     1501 ccggaaggcc cgtgaggtcc tgatgcggtt gggcctggac ccgactcagg aggactgcgt
     1561 ggccactcac cggatctgcc agatcgtgtc cacacgctcc gccagcctgt gcgcagccac
     1621 cctggccgcc gtgctgcagc gcatcaagga gaacaaaggc gaggagcggc tgcgctctac
     1681 tattggggtc gacggttccg tctacaagaa acacccccat tttgccaagc gtctacataa
     1741 gaccgtgcgg cggctggtgc ccggctgcga tgtccgcttc ctccgctccg aggatggcag
     1801 tggcaaaggt gcagccatgg tgacagcagt ggcttaccgg ctggccgatc aacaccgtgc
     1861 ccgccagaag acattagagc atctgcagct gagccatgac cagctgctgg aggtcaagag
     1921 gaggatgaag gtagaaatgg agcgaggtct gagcaaggag actcatgcca gtgcccccgt
     1981 caagatgctg cccacctacg tgtgtgctac cccggacggc acagagaaag gggacttctt
     2041 ggccttggac cttggaggaa caaatttccg ggtcctgctg gtccgtgttc ggaatgggaa
     2101 gtggggtgga gtggagatgc acaacaagat ctacgccatc ccgcaggagg tcatgcacgg
     2161 caccggggac gagctctttg accacattgt ccagtgcatc gcggacttcc tcgagtacat
     2221 gggcatgaag ggcgtgtccc tgcctctggg ttttaccttc tccttcccct gccagcagaa
     2281 cagcctggac gagagcatcc tcctcaagtg gacaaaaggc ttcaaggcat ctggctgcga
     2341 gggcgaggac gtggtgaccc tgctgaagga agcgatccac cggcgagagg agtttgacct
     2401 ggatgtggtt gctgtggtga acgacacagt cggaactatg atgacctgtg gctttgaaga
     2461 ccctcactgt gaagttggcc tcattgttgg cacgggcagc aatgcctgct acatggagga
     2521 gatgcgcaac gtggaactgg tggaaggaga agaggggcgg atgtgtgtga acatggaatg
     2581 gggggccttc ggggacaatg gatgcctaga tgacttccgc acagaatttg atgtggctgt
     2641 ggatgagctt tcactcaacc ccggcaagca gaggttcgag aaaatgatca gtggaatgta
     2701 cctgggtgag attgtccgta acattctcat cgatttcacc aagcgtggac tactcttccg
     2761 aggccgcatc tcagagcggc tcaagacaag gggcatcttt gaaaccaagt tcttgtctca
     2821 gattgagagt gactgcctgg ccctgctgca agtccgagcc atcctgcaac acttagggct
     2881 tgagagcacc tgtgacgaca gcatcattgt taaggaggtg tgcactgtgg tggcccggcg
     2941 ggcagcccag ctctgtggcg caggcatggc cgctgtggtg gacaggatac gagaaaaccg
     3001 tgggctggac gctctcaaag tgacagtggg tgtggatggg accctctaca agctacatcc
     3061 tcactttgcc aaagtcatgc atgagacagt gaaggacctg gctccgaaat gtgatgtgtc
     3121 tttcctgcag tcagaggatg gcagcgggaa gggggcggcg ctcatcactg ctgtggcctg
     3181 ccgcatccgt gaggctggac agcgatagaa cccctgaaat cggaagggac ttcctctttc
     3241 tctccttctt ccctgtttta aattataaga tgtcatcccc ttgtgtcaga gacagacccc
     3301 ttggcttttg cttggcagag aggaccccac tggactgggt tttgtctctg catctcattg
     3361 tagagcttgg tggctgagct tggccctatt aagataaata gagttccaaa taaggatttg
     3421 ttcacatgca tcataaccat tcccattggt tctcctaaaa catgaaaatt atctccctta
     3481 gtaatccccc ttgccaaatt ccatgtccct gtataattct acaggatggg gacactaatg
     3541 aagatacggt tgcttcacct tggagcctga acatgacatt tctaagtggg gtgcatcccc
     3601 cagcactgat gttgttactg attctcctgt cagagatctg ggaggtctcc actgaggatg
     3661 tgagcctgat tatcctatag gcagacgtgg ggagggtgga ggggtgacag tggaggaaaa
     3721 tccatggata tccacgcagc agcccctctt taacctcatc tacaagcatt tgccctgtgg
     3781 attccagcat ttgccattcc tggaatcaag gaatcctgag tctgggcaat gaaaccaaag
     3841 ccaggagttg acgcatcctg cagttgggcc agctgtcgca tctcagcggg gcgcacatgt
     3901 tatccacaag caatggacct ttggggaagg gggagttttt agtttgtttt acaaattttt
     3961 cctgcaaaag tggaatcact gtattttcat tttaatttat atttgaaatt ttatttagtt
     4021 cttgagtaga tctgcttctt catcttgaca tgtaatgaat ggtcagttgt acgtaatgta
     4081 tttatatgtt aatttgttat gtatatagat gtgcaagtct tgtcagaatt ggcctcagtg
     4141 tagttaaagg gcagaagggg aagatactga ctagtcatag aaatacctca ttcgcctgtg
     4201 ggaagagaag ggaagcctct tcagggtgag tgaatggcaa agcggttgct tctggctcct
     4261 ccttcccctg tggtcttgga agtgtgtgga aggcagggac agagatggag gccgagccaa
     4321 tagactgaag agaccacagc aattggctcc tccatctaga gattttcttg gcagtattcc
     4381 atgggatgtt aagcaaagga aaccaaagga atcgtttcaa atggactcat ggcttagaaa
     4441 tctttattct tagggcagtc agtagtattc taaagctttc tgacaagata aaggaagtca
     4501 ccaaaatttc tttttttaaa ttgtatctaa tcctcaacaa caaaccaaaa cagaacaatt
     4561 aaacagccaa ataaaacctc agggacaaca tttttggtgt atttgagccc tcccagcaag
     4621 tttcaccttg ggtttgtatt ttaaatgttt tacaagaatt gtccatgtgc ttccctaggc
     4681 tgagctggca ttggtctgct gacctgtttt tgtgtttttc ttttttttat acacaacatt
     4741 tatttcaaac tattgggagg gatgagagtg gcttaaaaac ttccatccct acttttcaag
     4801 agtgcagttg attctgaatc tgaaagcccg cctctgtcct aaaatacaaa caagcacaga
     4861 cattaaacct ggatactata tgataaagag ggatgtaact attgaattgg atacaaggat
     4921 cagaatggaa agaaactcac gatgaaattg aacctggttt ttgtatattt atcaaacttg
     4981 tgctgagaat agtgtctgat tatacgactt ttaagcaaag ttgggtgtaa ttaggtgaaa
     5041 acagcccagg tcctcccggg agcacagagg ggctaggggc tggtccttct cgtttgctct
     5101 agtcttgctt tgctgtctgg tgtagctcct ctgctgctcc catctgcact aattgaccca
     5161 aaacgtgggt atttcctgct acacaaaagc caaaaggttt catgtagatt ttagttcact
     5221 aaagggtgcc cacaaaatag agattaattt taacttaaat tttaagcttg aagattaggt
     5281 actatctgtg aagttacact tttttttttt tttttaaagg tagagatgtg tgtgtgtgta
     5341 ggtattaaag atgtgttgtt ggtttccaaa aaggaacact ggaaaataaa ttttgaatgt
     5401 ttatgttctc agaatcaggt tgacagtccc ttgctgacat ggctttgctt tgtgtaaata
     5461 cagtggatct caatcttcgg ggtgtgatga atagcgaatc atctcaaatc cttgagcact
     5521 cagtctagtg aagatgttgt cattatgtac aatacataac tagtttaatt aactatgtga
     5581 tgttaactat tattaataaa ttttaacatt ttccaaaata atcaaa
//
