LOCUS       NM_017902              12927 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens hypoxia inducible factor 1 subunit alpha inhibitor
            (HIF1AN), mRNA.
ACCESSION   NM_017902 NM_032215
VERSION     NM_017902.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 12927)
  AUTHORS   Liu,B., Rui,Y., Li,M. and Huang,L.
  TITLE     Cancer cell-derived exosomes promote NSCLC progression via the
            miR-199b-5p/HIF1AN axis
  JOURNAL   Mol Immunol 174, 32-40 (2024)
   PUBMED   39154583
  REMARK    GeneRIF: Cancer cell-derived exosomes promote NSCLC progression via
            the miR-199b-5p/HIF1AN axis.
REFERENCE   2  (bases 1 to 12927)
  AUTHORS   Li,N., Zhou,Q., Yi,Z., Zhang,H. and Zhou,D.
  TITLE     Ubiquitin protein E3 ligase ASB9 suppresses proliferation and
            promotes apoptosis in human spermatogonial stem cell line by
            inducing HIF1AN degradation
  JOURNAL   Biol Res 56 (1), 4 (2023)
   PUBMED   36683111
  REMARK    GeneRIF: Ubiquitin protein E3 ligase ASB9 suppresses proliferation
            and promotes apoptosis in human spermatogonial stem cell line by
            inducing HIF1AN degradation.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 12927)
  AUTHORS   Volkova,Y.L., Pickel,C., Jucht,A.E., Wenger,R.H. and Scholz,C.C.
  TITLE     The Asparagine Hydroxylase FIH: A Unique Oxygen Sensor
  JOURNAL   Antioxid Redox Signal 37 (13-15), 913-935 (2022)
   PUBMED   35166119
  REMARK    GeneRIF: The Asparagine Hydroxylase FIH: A Unique Oxygen Sensor.
            Review article
REFERENCE   4  (bases 1 to 12927)
  AUTHORS   Leissing,T.M., Hardy,A.P., Chan,H., Wang,Y., Tumber,A.,
            Chowdhury,R., Feng,T., Coleman,M.L., Cockman,M.E., Kramer,H.B.,
            Berridge,G., Fischer,R., Kessler,B.M., Ratcliffe,P.J., Lu,X. and
            Schofield,C.J.
  TITLE     Factor inhibiting HIF can catalyze two asparaginyl hydroxylations
            in VNVN motifs of ankyrin fold proteins
  JOURNAL   J Biol Chem 298 (6), 102020 (2022)
   PUBMED   35537551
  REMARK    GeneRIF: Factor inhibiting HIF can catalyze two asparaginyl
            hydroxylations in VNVN motifs of ankyrin fold proteins.
REFERENCE   5  (bases 1 to 12927)
  AUTHORS   Shi,Z., Li,G., Li,Z., Liu,J. and Tang,Y.
  TITLE     TMEM161B-AS1 suppresses proliferation, invasion and glycolysis by
            targeting miR-23a-3p/HIF1AN signal axis in oesophageal squamous
            cell carcinoma
  JOURNAL   J Cell Mol Med 25 (14), 6535-6549 (2021)
   PUBMED   34046994
  REMARK    GeneRIF: TMEM161B-AS1 suppresses proliferation, invasion and
            glycolysis by targeting miR-23a-3p/HIF1AN signal axis in
            oesophageal squamous cell carcinoma.
            Erratum:[J Cell Mol Med. 2023 Feb;27(4):591-592. doi:
            10.1111/jcmm.17661.. PMID: 36790069]
REFERENCE   6  (bases 1 to 12927)
  AUTHORS   Hewitson,K.S., McNeill,L.A., Riordan,M.V., Tian,Y.M., Bullock,A.N.,
            Welford,R.W., Elkins,J.M., Oldham,N.J., Bhattacharya,S.,
            Gleadle,J.M., Ratcliffe,P.J., Pugh,C.W. and Schofield,C.J.
  TITLE     Hypoxia-inducible factor (HIF) asparagine hydroxylase is identical
            to factor inhibiting HIF (FIH) and is related to the cupin
            structural family
  JOURNAL   J Biol Chem 277 (29), 26351-26355 (2002)
   PUBMED   12042299
REFERENCE   7  (bases 1 to 12927)
  AUTHORS   Lando,D., Peet,D.J., Gorman,J.J., Whelan,D.A., Whitelaw,M.L. and
            Bruick,R.K.
  TITLE     FIH-1 is an asparaginyl hydroxylase enzyme that regulates the
            transcriptional activity of hypoxia-inducible factor
  JOURNAL   Genes Dev 16 (12), 1466-1471 (2002)
   PUBMED   12080085
  REMARK    GeneRIF: FIH-1 is an asparaginyl hydroxylase enzyme that regulates
            the transcriptional activity of hypoxia-inducible factor
REFERENCE   8  (bases 1 to 12927)
  AUTHORS   Freedman,S.J., Sun,Z.Y., Poy,F., Kung,A.L., Livingston,D.M.,
            Wagner,G. and Eck,M.J.
  TITLE     Structural basis for recruitment of CBP/p300 by hypoxia-inducible
            factor-1 alpha
  JOURNAL   Proc Natl Acad Sci U S A 99 (8), 5367-5372 (2002)
   PUBMED   11959990
  REMARK    GeneRIF: Structural basis for recruitment of CBP/p300 by
            hypoxia-inducible factor-1 alpha
REFERENCE   9  (bases 1 to 12927)
  AUTHORS   Lando,D., Peet,D.J., Whelan,D.A., Gorman,J.J. and Whitelaw,M.L.
  TITLE     Asparagine hydroxylation of the HIF transactivation domain a
            hypoxic switch
  JOURNAL   Science 295 (5556), 858-861 (2002)
   PUBMED   11823643
REFERENCE   10 (bases 1 to 12927)
  AUTHORS   Mahon,P.C., Hirota,K. and Semenza,G.L.
  TITLE     FIH-1: a novel protein that interacts with HIF-1alpha and VHL to
            mediate repression of HIF-1 transcriptional activity
  JOURNAL   Genes Dev 15 (20), 2675-2686 (2001)
   PUBMED   11641274
  REMARK    GeneRIF: Gene name = FIH1Encoded protein (factor inhibiting HIF-1)
            is a co-repressor that interacts with hypoxia-inducible factor 1
            (HIF-1) alpha and the von Hippel-Lindau tumor suppressor protein to
            mediate repression of HIF-1 transcriptional activity.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL133352.12, AF395830.1 and AW015561.1.
            
            On Nov 22, 2018 this sequence version replaced NM_017902.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.170885.1,
                                           SRR1660805.138351.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000299163.7/ ENSP00000299163.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-6                 AL133352.12        72873-72878
            7-2696              AF395830.1         1-2690
            2697-6738           AL133352.12        86698-90739
            6739-6853           AW015561.1         17-131              c
            6854-12927          AL133352.12        90855-96928
FEATURES             Location/Qualifiers
     source          1..12927
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q24.31"
     gene            1..12927
                     /gene="HIF1AN"
                     /gene_synonym="FIH1"
                     /note="hypoxia inducible factor 1 subunit alpha inhibitor"
                     /db_xref="GeneID:55662"
                     /db_xref="HGNC:HGNC:17113"
                     /db_xref="MIM:606615"
     exon            1..193
                     /gene="HIF1AN"
                     /gene_synonym="FIH1"
                     /inference="alignment:Splign:2.1.0"
     CDS             17..1066
                     /gene="HIF1AN"
                     /gene_synonym="FIH1"
                     /EC_number="1.14.11.30"
                     /EC_number="1.14.11.n4"
                     /note="factor inhibiting HIF1; peptide-aspartate
                     beta-dioxygenase; FIH-1; factor inhibiting HIF-1;
                     hypoxia-inducible factor asparagine hydroxylase; hypoxia
                     inducible factor 1 alpha subunit inhibitor"
                     /codon_start=1
                     /product="hypoxia-inducible factor 1-alpha inhibitor"
                     /protein_id="NP_060372.2"
                     /db_xref="CCDS:CCDS7498.1"
                     /db_xref="GeneID:55662"
                     /db_xref="HGNC:HGNC:17113"
                     /db_xref="MIM:606615"
                     /translation="MAATAAEAVASGSGEPREEAGALGPAWDESQLRSYSFPTRPIPR
                     LSQSDPRAEELIENEEPVVLTDTNLVYPALKWDLEYLQENIGNGDFSVYSASTHKFLY
                     YDEKKMANFQNFKPRSNREEMKFHEFVEKLQDIQQRGGEERLYLQQTLNDTVGRKIVM
                     DFLGFNWNWINKQQGKRGWGQLTSNLLLIGMEGNVTPAHYDEQQNFFAQIKGYKRCIL
                     FPPDQFECLYPYPVHHPCDRQSQVDFDNPDYERFPNFQNVVGYETVVGPGDVLYIPMY
                     WWHHIESLLNGGITITVNFWYKGAPTPKRIEYPLKAHQKVAIMRNIEKMLGEALGNPQ
                     EVGPLLNTMIKGRYN"
     exon            194..444
                     /gene="HIF1AN"
                     /gene_synonym="FIH1"
                     /inference="alignment:Splign:2.1.0"
     exon            445..593
                     /gene="HIF1AN"
                     /gene_synonym="FIH1"
                     /inference="alignment:Splign:2.1.0"
     exon            594..739
                     /gene="HIF1AN"
                     /gene_synonym="FIH1"
                     /inference="alignment:Splign:2.1.0"
     exon            740..846
                     /gene="HIF1AN"
                     /gene_synonym="FIH1"
                     /inference="alignment:Splign:2.1.0"
     exon            847..910
                     /gene="HIF1AN"
                     /gene_synonym="FIH1"
                     /inference="alignment:Splign:2.1.0"
     exon            911..1021
                     /gene="HIF1AN"
                     /gene_synonym="FIH1"
                     /inference="alignment:Splign:2.1.0"
     exon            1022..12927
                     /gene="HIF1AN"
                     /gene_synonym="FIH1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1752..1757
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HIF1AN"
                     /gene_synonym="FIH1"
                     /note="hexamer: CATAAA"
     polyA_site      1777
                     /gene="HIF1AN"
                     /gene_synonym="FIH1"
                     /note="major polyA site"
     regulatory      2910..2915
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HIF1AN"
                     /gene_synonym="FIH1"
                     /note="hexamer: AATATA"
     polyA_site      2935
                     /gene="HIF1AN"
                     /gene_synonym="FIH1"
                     /note="major polyA site"
     regulatory      6821..6826
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HIF1AN"
                     /gene_synonym="FIH1"
                     /note="hexamer: AATAAA"
     polyA_site      6847
                     /gene="HIF1AN"
                     /gene_synonym="FIH1"
                     /note="major polyA site"
     regulatory      10326..10331
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HIF1AN"
                     /gene_synonym="FIH1"
                     /note="hexamer: TATAAA"
     polyA_site      10354
                     /gene="HIF1AN"
                     /gene_synonym="FIH1"
     regulatory      11924..11929
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HIF1AN"
                     /gene_synonym="FIH1"
                     /note="hexamer: ACTAAA"
     polyA_site      11963
                     /gene="HIF1AN"
                     /gene_synonym="FIH1"
     regulatory      12248..12253
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HIF1AN"
                     /gene_synonym="FIH1"
                     /note="hexamer: ATTAAA"
     polyA_site      12275
                     /gene="HIF1AN"
                     /gene_synonym="FIH1"
     regulatory      12900..12905
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HIF1AN"
                     /gene_synonym="FIH1"
                     /note="hexamer: ATTAAA"
     polyA_site      12927
                     /gene="HIF1AN"
                     /gene_synonym="FIH1"
ORIGIN      
        1 gtccctggcg gcggagatgg cggcgacagc ggcggaggct gtggcctctg gctctggaga
       61 gccccgggag gaggctggag ccctcggccc cgcctgggat gaatcccagt tgcgcagtta
      121 tagcttcccg actaggccca ttccgcgtct gagtcagagc gacccccggg cagaggagct
      181 tattgagaat gaggagcctg tggtgctgac cgacacaaat cttgtgtatc ctgccctgaa
      241 atgggacctt gaatacctgc aagagaatat tggcaatgga gacttctctg tgtacagtgc
      301 cagcacccac aagttcttgt actatgatga gaagaagatg gccaatttcc agaactttaa
      361 gccgaggtcc aacagggaag aaatgaaatt tcatgagttc gttgagaaac tgcaggatat
      421 acagcagcga ggaggggaag agaggttgta tctgcagcaa acgctcaatg acactgtggg
      481 caggaagatt gtcatggact tcttaggttt taactggaac tggattaata agcaacaggg
      541 aaagcgtggc tgggggcagc ttacctctaa cctgctgctc attggcatgg aaggaaatgt
      601 gacacctgct cactatgatg agcagcagaa cttttttgct cagataaaag gttacaaacg
      661 atgcatctta ttccctccgg atcagttcga gtgcctctac ccataccctg ttcatcaccc
      721 atgtgacaga cagagccagg tggactttga caatcccgac tacgagaggt tccctaattt
      781 ccaaaatgtg gttggttacg aaacagtggt tggccctggt gatgttcttt acatcccaat
      841 gtactggtgg catcacatag agtcattact aaatgggggg attaccatca ctgtgaactt
      901 ctggtataag ggggctccca cccctaagag aattgaatat cctctcaaag ctcatcagaa
      961 agtggccata atgagaaaca ttgagaagat gcttggagag gccttgggga acccacaaga
     1021 ggtggggccc ttgttgaaca caatgatcaa gggccgatac aactagcctg ccaggggtca
     1081 aggcctcctg ccaggtgact gctatcccgt ccacaccgct tcattgatga ggacaggaga
     1141 ctccaagcgc tagtattgca cgctgcactt aatggactgg actcttgcca tggcccagga
     1201 gtcaggtgtt tggagcgagg cagggcagtt ggcactccac tcctatttgg agggacttca
     1261 tacccttgcc tcttgtgccc cagcaccttc tctctctgcc ccccgcctaa agtcctgcat
     1321 tcagtgtgtg gagtcccagc ttttggttgt catcatgtct gtgtgtatgt tagtctgtca
     1381 acttcggaat gtgtgcgtgt gtgtgcatgc acacgcatgt atgtatctgt tccctgttcc
     1441 ttctgggtca ggctgtcact tccggctctc agccctatct cctgcaacct cagtgcctca
     1501 gcctgagaga gagatgagat gctcttggac tccccactgc atctgggctg cagggccaga
     1561 gctagtctga ccattaggtc agtctgcctc ctgacagttt ttgcgtagtc aagctctagg
     1621 cggtatggga atggctaccg ggactctaat ggggtgaaag agaggggagg cttgcctttg
     1681 agagcctata tagccttcct gtgagagagg attagatagg gttccaactg ggcctacaag
     1741 ctcaagccat acataaaagg accttgggac ataagaacca atgattgtgc ataagttcta
     1801 aattagagac acatatagtt tctctctttc agcaccagct cttgccccta tgctgggtac
     1861 caagggagtt ctcctagctg tggcttctct aggttctagg ggtgcaagcc tctgtgtgtt
     1921 tgtttgtgtg tgtctgtgtg tgcgtatcca cactaggggt gcaagcctct gggtgtgtgt
     1981 gtgtgtgtgc gtgcgtgtgt gtgtgtgtgt ccgtgtgtgt gtgtgtgtgt gtccacactg
     2041 gccagcctcc ctacttacca aggttctcca ctgcttacct tttccagtgg gacagtacag
     2101 tgtgagcccc cgggaagtac tgcctgacct atcctaagct tttacacttg gattttagcc
     2161 atcatatgtt ggccaggtct cactgcagcc tgcccgaggc taactggcta gagcctccag
     2221 gccctatgat gctccctgcc caggccatat cctttattcc tgctgagctt cctggctgaa
     2281 tagatgaaat ggggtcaagc ccaggcagct cattcactac ctgtgatcca cctcagggca
     2341 cgggcaaaca cataggcttg cgtcttaaag ccagctcctc tgccagaccc cgttgtaatg
     2401 tgccacaaca ccctcaatag tcagggcaac tggtggagca tggaagtcga atttcctttt
     2461 ctgttaggag ctactcctgg gaacccctct cagggctgca gcttacaggt gggcagctgt
     2521 gattgcacaa cttgaagggc catcattcac atctattcag tgggagtggg gtccctggga
     2581 ttgggcagtg tggtggccct gtgtctcctc acctctgctc ctgtcttcat caccttctct
     2641 ctggaaggga agaggagttg gaaggtctct ggttttcttt tctttttttt tttttttgcc
     2701 aaaggtttac ttccagcatc tgagctctgg ctctcacccc tgaagctcag ttatagtgca
     2761 ctgatgaact gagaggatgc gtgtggatgt gtgtgcatgc ctgagtgcgt tttttgggga
     2821 ggggtgttta tttttagtac cccattctgg ggttctctga tgcagtgtgg atgtgaagat
     2881 atggtacctt ctcaagtgta gctctttcaa atatagtcaa tgctgggaaa tgtgattgca
     2941 gtgatctcta tctctccact tcttttggga aagaggagca caggagcgga ggaaaaactt
     3001 ggccacagtc acacttggaa agatagtaga ttattttcgt tctcctcagc aggtctgctg
     3061 tattcctcgc tcagcgctca aagatgtggg tgatgtgtca aggaggcatg cactcagctg
     3121 gtccttgctg agttatttgc tgacacaggt ctcactaagg tggctgaggg gtgggaggga
     3181 gaaggctaat cttggaacct tcacaggatt ggccttgatc cttgggtaac acaagagtga
     3241 ttcctgattt ttagacacct aatatgtgcc tagtgctttt ctctttgttc ctcactatcc
     3301 tgcaaggtag gtattctcac ttacagatga ataaatgggc tcagagagat taggtgatta
     3361 gtgacaatca gttgtaactt agcactgtta ggctgcaaac cccttgctcc ttcctttcca
     3421 ccaaactatg ttgattttcc ttccttacct ccctccttgt accacaggtt ctgtcctcaa
     3481 gatatcaccc cactgggcag tatctgaagg cagacccagc ccaagatggt gcagaatata
     3541 cagctcaggg tgaaagttct tctgggctcc tgctaacaaa gtcttggaaa aactgcctgg
     3601 gaagccatat tatgaattag tgctagaaga agctgtgggg ggaactgagt tctgtgtcta
     3661 attgcattac ttatgtgtcc ttgaatagga cccataactc ctcccggcat gcttttgcat
     3721 ctatagaact gaagctgtag ttctagatgg cactaagggt cttggttcat attcagctgc
     3781 tgcttccccc caaggaaaag atgaggtttt tccccttcga ttaccttttg agggatgttt
     3841 aggctcaaat tctgcttgcc acagaggaaa catttctgct gtagttgttt acctccatcc
     3901 tagctggcag tgtgggacac acacatacag caagtgaagg tgggggtttg ggcctgtata
     3961 gaggtcagct acgctgcagg ctcatctatt cacaccagca ggtacagaga aaactagtgt
     4021 tttgtaaagt caaatatttg ttgggggttg gagttctggg gttggaggag gggctgttct
     4081 gggcatcagt tgagcagatg cccaggatgc ctgggggaga ccagcttccc ctacaaatca
     4141 gagctctaaa ttacaaggtt tttaccaacg tgaacagttg ggggaagtcg tctgctctca
     4201 tttgcgtaat ggtttctgtc actggtgatt agacacagga tgaaggaaaa gaaatttgac
     4261 aattaggaat gagcgatcat taatctgaat ctgttaggag acagagaggg gaaggatcct
     4321 tatcagtggg ccagcccagt atggggagac actccctccc tctgccccca gagactcctg
     4381 ggctacatcc tctttcagta ttgccacaag tgggcagagc ttgtatttct taacaaagat
     4441 tagggaccca gttgccagcc tgagatggat ataggaacag acatctttgg gcatgagcca
     4501 acaaagatag aaatagatga gtgtcaggta caagctataa ggcaaagaat aacagtggaa
     4561 aagttttgac atgtgtttgc catttgtgga aaatgttttt gtaatagtca acacccctca
     4621 tagcccacct gtgacaagta cttgttacat tccagcactg tagggcgtga ggaaaatctg
     4681 gtccttatca aatcccagga gcttctgctt agttggggaa gaaattacat gaagcaacca
     4741 gaggttataa ggccacactt gtatatcgtg caccctgtgt ggacaagatt agggactgtt
     4801 gagagaggag gaaaccagta gagagcaaag ctctacccag gctccttgta agcctctggg
     4861 ctcccccgag agggcctcgc tactctacgc ttcctagcaa cgttgatgtc cccacaaccc
     4921 cacatcagtg cagctgtggc tgtgtggagg ggctctgagg cctctgaggc cagatgtgta
     4981 aacagtgctg aggttcagta ataggatgag tcttcaggtg tggagcagcc caccttggct
     5041 cttcccatgt ctctgtgtta cttctcatat tctgctgtcc tttcaaactt caaggacagt
     5101 attaatttat actagtattt cttcctcagt tttgtgactt gaatgcagtg agtgccttag
     5161 aggatccaag gatgaaggaa tgcgggttgg tggttctctc tttcagaatg ggaacttccc
     5221 aaaaatgggg ctgcgtctcg cctctcagta ggttccctac ctctgggtct tccacccttc
     5281 aaaatctggt acagaattta gcaggggctg cagggaatga ccctcaggga ccagtttcac
     5341 ccagatgggg tagatgatga ggtataccta gaagtagctc agggctccag agtctggcct
     5401 ttcagctggt gtcttactcc cctttgagac cttcagctgc ttgtcttcca ctgcctgcat
     5461 acttctctga tgtcttctca aactgttggc tctaaaggga tgttccagcc tccctctcca
     5521 gagctgcctg cagtttccct tattcagctg tctcctgcct agtctctcca gcggcctcct
     5581 taccaagacc ctttttccta aacagttgtc cttccctcag aaactccatg gctgcaagtg
     5641 tgaatagact tgtctttagg atccctctct ctggtcatcc ctgatcatct tagggccgtc
     5701 tcccttttct gatttttgac agctctgttt attttgccca tggctagtcc tcttgttttg
     5761 tgtctgctgt gtgttcttgc ccttccattg tcagttctgt acatcctggt tcagcttgtc
     5821 tcactgtggt agccccttct gcttgtcttc agccatcttg gtttaatcct cataggtcat
     5881 ggatgaatgg agccttgctg agcactttga gggcatttct gttgggcaca acctcagaaa
     5941 cgggaagtct tgaggcctgg gggcgtgtgg tcagtgaggc ctccagaggt gggttagggg
     6001 tgggcaccta caagggccag ttgaggggag aggtgcaggg attgctgagg agactttgtg
     6061 agaatgatgt caggcccggg aagctggtgt ggaagaactg agctgtcaga aggagatacc
     6121 aatatcccct ttgcagggtg gtgatgggca tcaaacatga cgtggaacat gtccccttca
     6181 tggagggttt ccatctaaat gcatctgtgt gccttaggga tcagtagttc aggggtggtg
     6241 gacttttttg agtgtctcct aatttctctg aggtgtcttt ggctctgtgt caccaaatgt
     6301 gcctgacctc tgcatcccag tggttgctga aagggaaaca gtatattgcg tagtcagatg
     6361 tcaggagtag agatcttaat gagaaaggcc ctggcagctg gagagatgag aggacctagt
     6421 ggtcagctta tacccagact atctctccac tcctccaaga gtttgagtcc catagctgat
     6481 gaaagaattt acatatgggc tcccccctgc tttcccactg aaaatctatt ctcatatttt
     6541 gggaaggagc atatgtcctt taactttcaa tggtaaacaa tctaggatag tgtttaaagg
     6601 catggatttt ggagtcacat atagttggtt tgaatttcta gcttcactac ttaccagctg
     6661 ggtttgacct tggttgattt acttaacctc tctgagcctc agtttcctca actataataa
     6721 catgggtgga gggaggcatg ttgaaaatat ctacctcagg gttgttggaa taactgaaac
     6781 aatgtttgta aagctttagc acagtgcctg gcaagcactt aataaatggc tgtggtggtg
     6841 gtggttatat ttattatgtg atttattcag aaacttggtt tcagtgatct ctaaacagat
     6901 ttccagtggt tctttctctc tcaagaaaag catagggaaa aacgagaaac accttagata
     6961 aaccatattg agttgagggt agacagaaga gtctagtacc taaactcacc ctagaggaag
     7021 agaaacagga agtgtcttat gcctcttctg cctgggcagc cagactttac tgctgtacta
     7081 ccagcccagg gccttgggtg agaaggtttc aggtctgagc tccccttgca ctgtgtgaga
     7141 ggccgtggag ccgaatgagt ccttattata cctggagaag ctgttgtctc ctcacaccat
     7201 gaaatgtaat agcagtcaaa tcaggcccct cttgattcaa ttttagtcca tgcctttcca
     7261 tgtatttaaa ttatataagt gttggccagg tgtggtggct cacacctgta atcccagcac
     7321 ttcgggaggc tgaggcagga ggatcacttg agcccaggag ttcgaaccaa ccttgggcag
     7381 catagtgaga ccccttctct taataatttt ttttgaaaat aattttttaa aaattacaaa
     7441 tatggaccag acacctggga ggattacctc acgcctgtaa tcccagcact ttgggaggct
     7501 gaggtgggtg gatcacctga ggtcaggagt ttgagactat cctggccaac atggtgaaac
     7561 cctgtctcta ctaaaaatac aaaaattagc cagatgtggt ggtgggtacc tgtaatccca
     7621 gctactcggg aggctgagac aggagaatca cttgaacctg ggaggcggag gttgcagtga
     7681 gctgagatca cgtcattgca ctccagcctg ggtgactaca gtgagactcc ctctcaaaaa
     7741 aaaaaaaaaa aaattatgag tatgttaaga ttgttctagg ggagaagggc aatcaaaatt
     7801 cagaaaaata tgagtgggat gttaatagct accatttgtt gaacacttct gttccaggca
     7861 ctgtattagt tcgtccccat gtggtgctct cagatcagta ggggagacag gcaggcagtt
     7921 agaattatgg tgtatagagg agaagcattg cactatgtcc agttttttat ggtaggcttc
     7981 ctgcagaaag tgataagtcc aagctgatat gtggaagatg aataattaac cagaggttgg
     8041 gggatttccc ccttagcttc tctttcttcc agtccagcag tccaaaacaa acactgcttc
     8101 ccaatgccta agggttttgt cgtaccttac tccagggaat gggtgagaca gtagatccca
     8161 agaagaaaac cctttggtta gtcacagaaa tctgaaagtc ttggttgctg gctcagattt
     8221 gtcctggagt ggacgtagat tggacttgct tatttgtagc cctccaccgt tctcatttgt
     8281 gttctggcct ctggagccac cctgccagac cctacttgga agaaatgttg aatcaagcta
     8341 ctcagacttc agcctgagat gtattatggc tggctggaat tctcagccct ttccacatca
     8401 tgatcccatt aatctcttgg gtctatcccc acgcccagcc acctctcctc ctcactataa
     8461 gccaagtctc cctgcagttc tctataaata ctcagatcct cttgcctcag accctggtcc
     8521 ctagctttgt gggtgaagaa gatacctctt tagcttgtgc caacccatcc ctctgggcta
     8581 caggccattc tgtagtcacc ttgcagtcac gtctttcctt ctagataact ggctctgggc
     8641 cttggaaacc ttctctttgg ctctagctaa ttttgctgtt ttattccgtt tacttaaaac
     8701 ctattcactt ggtcattgag gtcagaacta tcaggaaagc tgtgctcctt aacccatcat
     8761 ctaagctaag cttgggccag gtttttatta cctcttgaat atatatagtg caatatagtg
     8821 tagtggttga gagtggagtc tgcatacctg gatctgccat tctgcatgac tttgggcaaa
     8881 tggacaaact gcccgaaact tgacttgttt atatgtatgg ggaagacatc atttacattg
     8941 tcagggtaag gcttagcaga atgaccggtc catagcaagg gctcagtctt tctgtgtctc
     9001 tcagtcttta cctctcttcc ccatctagct ttctgtctaa gttcctgttt ctagtctcag
     9061 ggcatatcta gttgtcagct cctgagcaga gcagaggtat aagtgtcaac tcatgcttgt
     9121 gttaccagtt cctacccctg tcagtaacct ctgatgctta caaggatact atcacctatg
     9181 ccatcagtgg ggaatgacct tccaaaaata tttttaacaa tactaaaaat acctgaaaca
     9241 tactgggtga aaataagaca ttggagctgc tgctccccaa ggagataaaa ggacagttcc
     9301 taaagaggca ttgaaggttt gtatgagcca gcagcacagt cagaaggcaa gaaggagacc
     9361 ctgggatgtg cacaaggatg caggagacga gattgtcaca gaggctgtca tgctagtaag
     9421 agtcagaggt tactgatggg tagagattgt cagagtctgc cttgctagtc tagaggtagg
     9481 accatggatt taagaacgtt gcctccaagt ttttgaattg tgaatttttg atcatatttg
     9541 aacaaaaccc cacctacagt ctgcatggtc attgttctca caagggtttg tgtgatgcac
     9601 tgacaagaac agaggctttg gaggtgactc ctgggtttga atcaccattt gccactagct
     9661 aattctaacc ttaggtaagt cagtgtctct gggtctcaac ctcttcctct gtgaggggta
     9721 ggaaatagca cataacttgt agcattgtta taagggctcg tgataatgtt tttaaaacac
     9781 ctggctcaag cactcaggaa aatgttttat tatgaagacc aagtgtctct gaaaagtgtt
     9841 tccttagtgt ctggagagac tacagcagca aggcttttct gatcctactc tgactctcaa
     9901 gataatggtg cttgaagtga aatcacagaa gagatgactg gggcaggaaa agcaagatgg
     9961 aaaagataaa gttgtggaag ggactgaaag tccatgaagt gctgggctgg ggacagaaag
    10021 ggtttaatag ggcagggtct tgaggaacag gaacagagac cttggttctg ggttgccatc
    10081 tgattcccag aagagtaaag gaaatggggc aggaggatgt ctgtggagga cacagtgtgg
    10141 ctgagtggag agaacacaag cttctggcca gtgaacctaa gtgagaatgt cagtggactt
    10201 tgcacaggct gggtgccttt gggggtgttc atctcgctat gcttcggtcc cctcatttaa
    10261 aaaaattggt taataatatg atgacctgcc aatgtgtgtg gaaatactat gtaaactgag
    10321 cactatataa atgctggttg ttgagatatt gggagtgggg gtttcgggag attttgtttg
    10381 tttgtttttt tgagatggag ttttcgctct tgttgcccag gctggagtgc agtggcgtga
    10441 tcttggctca ctgcaacctc cacctcctcc aggttcaagc aattctgcct cagcctcccg
    10501 agtagctggg actcaggcgt gcgccaccat gcctggctaa ttttgtattt ttagtagaga
    10561 tggggtttct ccatgttggt caggctggtc tcgaactccc gacctcaggt gatctgcctg
    10621 ccttggcctc ccaaagtgct gggattacag gtgcgagcta ccacacctgg ccctggattt
    10681 cttaataggt cctcttatgt ggctattgag aatgaatgag acaactcatt aactaactaa
    10741 gttaattcag aacttagtgc ctagtaagtg ctcagtaaat gttagtttct tttcctccat
    10801 ttcttcttag gaagcatcgt ttacccagca taaacaggag gactcaactg tgcatgtctt
    10861 agtgtgaaca agtaacatca gatttccctt ttctgaggag agatcagttc tgtaagaggg
    10921 agcatgagtg tgaatgggga agaatcatgg agaagggagc aggactccac agtactggag
    10981 atggagtagg gacctctggg aatcgttggc atgggggtgt ggtgcagaaa gggtgatgct
    11041 aagaggtgag agtctcaaga aagaagatgg gtgttctaga ggggaaagct aaacgatgat
    11101 aggcaatgag gagcggagga caagaccctg aactaagggt gaggaggcca gcattctgtc
    11161 aggagaacct taggaattgt gaccagaagt caaagttgtt acagcaatcc tgcattcaac
    11221 caggtttcat ctgaaaaact taagagttgc taggcacagt cgtgcacact tgtaatccca
    11281 acactttggg aggcttgagt caggaggaag cttgaggcca ggagtttgag atcagcctag
    11341 gcagcatagt gacaccccat ctcttgaaaa aacaaacact cttaagagca gtggttcacc
    11401 tagaaataaa aagcagaaat catcaagatg agaagacatt ttgtgttttg acagccaagg
    11461 aaatttgcca ctgagacaag gtatctgaca gcctagatgg gattctagac ttctgtgaga
    11521 aagtgcagtt aggccctgaa tagcgtcaaa ctcagggatg tgagctgact gactgccttt
    11581 ttatgtaatg gaggccatgt gatagggctt tatgaggcca caaatagcac tttctgggtc
    11641 ctttcacagt ctagtcagtg gcccaaccaa catatctggt tgttctgcaa tctcagtcat
    11701 ctgagaagat aacctccaaa atacatgcag tggcatcagg tgtttccttg gtaggtgtgc
    11761 atcttttaat gatggaggac ttaggatgaa gaacaggtgt gtttaaaaac atcaaacccc
    11821 cacatcatta ctatttgctg taaggatcta tgcagtttgt attttaagtc cttgacatat
    11881 ggaagagcat ttgtgagagt aacaggtttg ctttgtaatg ccaactaaaa agtgtgtatt
    11941 tatactcgaa ttttaagttg aaattttgct aagtttcttt tttttttttt tgagacggag
    12001 tctcactctg ttgcccaggc tggagtacag tggtacaatc attgctaagt ctttctgtag
    12061 ttttagaaca tttagaacaa cttaagattt cctgtattta taaacttaat ttattggaaa
    12121 tataagtact tgatcaggtt ttgtcttggt cagataaatt aagtacttaa ggaaatcaaa
    12181 tacattaatc atataaatgc catttaagtg tttaaaggag tttagttaaa tgtaagttgg
    12241 atcaaacatt aaactttttt gttttataag caaaataata agatttgtta gcagaactta
    12301 aaagcttaga aaagagagga ttttggatat gtaactttga agaggtgaat agtttgctct
    12361 tttctgagac agggcattgc ttttcgacgc aggctggagt gcagtggggc aagcatggct
    12421 cactgcagcc tcaacctcct ggactcaagt gatcctcctg cctcagcctc ccatgtagct
    12481 gggatcacag gcacatgcca ccacacctgg ctaatttttt attttttgta gagacagggg
    12541 cctcacttca ttgcccaggc ggatctcgaa cacctgggct caagcagtcc tgcctcagcc
    12601 tcccaaagtt ctgggattac aggcataagc cactgtgcct ggccttgaat atatttttta
    12661 accaaagtaa tttatgaata taaattgttt ctatataacc caaagtcact ctcattgaca
    12721 ttaagaaact cagagagtgg gttctaaatg taatattacc acacacccaa gtcacttagc
    12781 ctctacaagt accacgtaac tagcagccgt taaaagatgg ccaccgctag tccagtcggc
    12841 cacagacatg gttttgtttg gccaatacaa gtttttgaaa gttttaaaaa attgacaaca
    12901 ttaaagatca gaaacttgac ctaaaaa
//
