LOCUS       NM_004712               2893 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens hepatocyte growth factor-regulated tyrosine kinase
            substrate (HGS), mRNA.
ACCESSION   NM_004712
VERSION     NM_004712.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Trepte,P., Secker,C., Kostova,S., Maseko,S.B., Choi,S.G.,
            Blavier,J., Minia,I., Ramos,E.S., Cassonnet,P., Golusik,S.,
            Zenkner,M., Beetz,S., Liebich,M.J., Scharek,N., Schutz,A.,
            Sperling,M., Lisurek,M., Wang,Y., Spirohn,K., Hao,T.,
            Calderwood,M.A., Hill,D.E., Landthaler,M., Olivet,J., Twizere,J.C.,
            Vidal,M. and Wanker,E.E.
  TITLE     AI-guided pipeline for protein-protein interaction drug discovery
            identifies a SARS-CoV-2 inhibitor
  JOURNAL   bioRxiv (2023)
   PUBMED   37398436
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Xiao,B.L., Wang,X.L., Xia,H.F., Zhang,L.Z., Wang,K.M., Chen,Z.K.,
            Zhong,Y.H., Jiang,H.G., Zhou,F.X., Wang,W., Chen,G.L. and Chen,G.
  TITLE     HRS Regulates Small Extracellular Vesicle PD-L1 Secretion and Is
            Associated with Anti-PD-1 Treatment Efficacy
  JOURNAL   Cancer Immunol Res 11 (2), 228-240 (2023)
   PUBMED   36484721
  REMARK    GeneRIF: HRS Regulates Small Extracellular Vesicle PD-L1 Secretion
            and Is Associated with Anti-PD-1 Treatment Efficacy.
REFERENCE   3  (bases 1 to 2893)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   4  (bases 1 to 2893)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 2893)
  AUTHORS   Gutmann,D.H., Haipek,C.A., Burke,S.P., Sun,C.X., Scoles,D.R. and
            Pulst,S.M.
  TITLE     The NF2 interactor, hepatocyte growth factor-regulated tyrosine
            kinase substrate (HRS), associates with merlin in the 'open'
            conformation and suppresses cell growth and motility
  JOURNAL   Hum Mol Genet 10 (8), 825-834 (2001)
   PUBMED   11285248
REFERENCE   6  (bases 1 to 2893)
  AUTHORS   Chin,L.S., Raynor,M.C., Wei,X., Chen,H.Q. and Li,L.
  TITLE     Hrs interacts with sorting nexin 1 and regulates degradation of
            epidermal growth factor receptor
  JOURNAL   J Biol Chem 276 (10), 7069-7078 (2001)
   PUBMED   11110793
REFERENCE   7  (bases 1 to 2893)
  AUTHORS   Scoles,D.R., Huynh,D.P., Chen,M.S., Burke,S.P., Gutmann,D.H. and
            Pulst,S.M.
  TITLE     The neurofibromatosis 2 tumor suppressor protein interacts with
            hepatocyte growth factor-regulated tyrosine kinase substrate
  JOURNAL   Hum Mol Genet 9 (11), 1567-1574 (2000)
   PUBMED   10861283
REFERENCE   8  (bases 1 to 2893)
  AUTHORS   Lippincott-Schwartz,J., Roberts,T.H. and Hirschberg,K.
  TITLE     Secretory protein trafficking and organelle dynamics in living
            cells
  JOURNAL   Annu Rev Cell Dev Biol 16, 557-589 (2000)
   PUBMED   11031247
  REMARK    Review article
REFERENCE   9  (bases 1 to 2893)
  AUTHORS   Lu,L., Komada,M. and Kitamura,N.
  TITLE     Human Hrs, a tyrosine kinase substrate in growth factor-stimulated
            cells: cDNA cloning and mapping of the gene to chromosome 17
  JOURNAL   Gene 213 (1-2), 125-132 (1998)
   PUBMED   9630564
REFERENCE   10 (bases 1 to 2893)
  AUTHORS   Asao,H., Sasaki,Y., Arita,T., Tanaka,N., Endo,K., Kasai,H.,
            Takeshita,T., Endo,Y., Fujita,T. and Sugamura,K.
  TITLE     Hrs is associated with STAM, a signal-transducing adaptor molecule.
            Its suppressive effect on cytokine-induced cell growth
  JOURNAL   J Biol Chem 272 (52), 32785-32791 (1997)
   PUBMED   9407053
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC003565.1 and AI365104.1.
            
            On Nov 22, 2018 this sequence version replaced NM_004712.4.
            
            Summary: The protein encoded by this gene regulates endosomal
            sorting and plays a critical role in the recycling and degradation
            of membrane receptors. The encoded protein sorts monoubiquitinated
            membrane proteins into the multivesicular body, targeting these
            proteins for lysosome-dependent degradation. [provided by RefSeq,
            Dec 2010].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660803.27635.1, D84064.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000329138.9/ ENSP00000331201.4
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2890              BC003565.1         22-2911
            2891-2893           AI365104.1         15-17               c
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q25.3"
     gene            1..2893
                     /gene="HGS"
                     /gene_synonym="HRS"
                     /note="hepatocyte growth factor-regulated tyrosine kinase
                     substrate"
                     /db_xref="GeneID:9146"
                     /db_xref="HGNC:HGNC:4897"
                     /db_xref="MIM:604375"
     exon            1..93
                     /gene="HGS"
                     /gene_synonym="HRS"
                     /inference="alignment:Splign:2.1.0"
     CDS             57..2390
                     /gene="HGS"
                     /gene_synonym="HRS"
                     /note="human growth factor-regulated tyrosine kinase
                     substrate; protein pp110"
                     /codon_start=1
                     /product="hepatocyte growth factor-regulated tyrosine
                     kinase substrate"
                     /protein_id="NP_004703.1"
                     /db_xref="CCDS:CCDS11784.1"
                     /db_xref="GeneID:9146"
                     /db_xref="HGNC:HGNC:4897"
                     /db_xref="MIM:604375"
                     /translation="MGRGSGTFERLLDKATSQLLLETDWESILQICDLIRQGDTQAKY
                     AVNSIKKKVNDKNPHVALYALEVMESVVKNCGQTVHDEVANKQTMEELKDLLKRQVEV
                     NVRNKILYLIQAWAHAFRNEPKYKVVQDTYQIMKVEGHVFPEFKESDAMFAAERAPDW
                     VDAEECHRCRVQFGVMTRKHHCRACGQIFCGKCSSKYSTIPKFGIEKEVRVCEPCYEQ
                     LNRKAEGKATSTTELPPEYLTSPLSQQSQLPPKRDETALQEEEELQLALALSQSEAEE
                     KERLRQKSTYTSYPKAEPMPSASSAPPASSLYSSPVNSSAPLAEDIDPELARYLNRNY
                     WEKKQEEARKSPTPSAPVPLTEPAAQPGEGHAAPTNVVENPLPETDSQPIPPSGGPFS
                     EPQFHNGESEESHEQFLKALQNAVTTFVNRMKSNHMRGRSITNDSAVLSLFQSINGMH
                     PQLLELLNQLDERRLYYEGLQDKLAQIRDARGALSALREEHREKLRRAAEEAERQRQI
                     QLAQKLEIMRQKKQEYLEVQRQLAIQRLQEQEKERQMRLEQQKQTVQMRAQMPAFPLP
                     YAQLQAMPAAGGVLYQPSGPASFPSTFSPAGSVEGSPMHGVYMSQPAPAAGPYPSMPS
                     TAADPSMVSAYMYPAGATGAQAAPQAQAGPTASPAYSSYQPTPTAGYQNVASQAPQSL
                     PAISQPPQSSTMGYMGSQSVSMGYQPYNMQNLMTTLPSQDASLPPQQPYIAGQQPMYQ
                     QMAPSGGPPQQQPPVAQQPQAQGPPAQGSEAQLISFD"
     exon            94..178
                     /gene="HGS"
                     /gene_synonym="HRS"
                     /inference="alignment:Splign:2.1.0"
     exon            179..254
                     /gene="HGS"
                     /gene_synonym="HRS"
                     /inference="alignment:Splign:2.1.0"
     exon            255..347
                     /gene="HGS"
                     /gene_synonym="HRS"
                     /inference="alignment:Splign:2.1.0"
     exon            348..471
                     /gene="HGS"
                     /gene_synonym="HRS"
                     /inference="alignment:Splign:2.1.0"
     exon            472..524
                     /gene="HGS"
                     /gene_synonym="HRS"
                     /inference="alignment:Splign:2.1.0"
     exon            525..593
                     /gene="HGS"
                     /gene_synonym="HRS"
                     /inference="alignment:Splign:2.1.0"
     exon            594..718
                     /gene="HGS"
                     /gene_synonym="HRS"
                     /inference="alignment:Splign:2.1.0"
     exon            719..797
                     /gene="HGS"
                     /gene_synonym="HRS"
                     /inference="alignment:Splign:2.1.0"
     exon            798..896
                     /gene="HGS"
                     /gene_synonym="HRS"
                     /inference="alignment:Splign:2.1.0"
     exon            897..992
                     /gene="HGS"
                     /gene_synonym="HRS"
                     /inference="alignment:Splign:2.1.0"
     exon            993..1031
                     /gene="HGS"
                     /gene_synonym="HRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1032..1175
                     /gene="HGS"
                     /gene_synonym="HRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1176..1235
                     /gene="HGS"
                     /gene_synonym="HRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1236..1449
                     /gene="HGS"
                     /gene_synonym="HRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1450..1622
                     /gene="HGS"
                     /gene_synonym="HRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1623..1763
                     /gene="HGS"
                     /gene_synonym="HRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1764..1938
                     /gene="HGS"
                     /gene_synonym="HRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1939..2072
                     /gene="HGS"
                     /gene_synonym="HRS"
                     /inference="alignment:Splign:2.1.0"
     exon            2073..2192
                     /gene="HGS"
                     /gene_synonym="HRS"
                     /inference="alignment:Splign:2.1.0"
     exon            2193..2279
                     /gene="HGS"
                     /gene_synonym="HRS"
                     /inference="alignment:Splign:2.1.0"
     exon            2280..2893
                     /gene="HGS"
                     /gene_synonym="HRS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2871..2876
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HGS"
                     /gene_synonym="HRS"
                     /note="hexamer: AATAAA"
     polyA_site      2893
                     /gene="HGS"
                     /gene_synonym="HRS"
                     /note="major polyA site"
ORIGIN      
        1 gcgccagctc gtagcagggg agcgcccgcg gcgtcgggtt tgggctggag gtcgccatgg
       61 ggcgaggcag cggcaccttc gagcgtctcc tagacaaggc gaccagccag ctcctgttgg
      121 agacagattg ggagtccatt ttgcagatct gcgacctgat ccgccaaggg gacacacaag
      181 caaaatatgc tgtgaattcc atcaagaaga aagtcaacga caagaaccca cacgtcgcct
      241 tgtatgccct ggaggtcatg gaatctgtgg taaagaactg tggccagaca gttcatgatg
      301 aggtggccaa caagcagacc atggaggagc tgaaggacct gctgaagaga caagtggagg
      361 taaacgtccg taacaagatc ctgtacctga tccaggcctg ggcgcatgcc ttccggaacg
      421 agcccaagta caaggtggtc caggacacct accagatcat gaaggtggag gggcacgtct
      481 ttccagaatt caaagagagc gatgccatgt ttgctgccga gagagcccca gactgggtgg
      541 acgctgagga atgccaccgc tgcagggtgc agttcggggt gatgacccgt aagcaccact
      601 gccgggcgtg tgggcagata ttctgtggaa agtgttcttc caagtactcc accatcccca
      661 agtttggcat cgagaaggag gtgcgcgtgt gtgagccctg ctacgagcag ctgaacagga
      721 aagcggaggg aaaggccact tccaccactg agctgccccc cgagtacctg accagccccc
      781 tgtctcagca gtcccagctg ccccccaaga gggacgagac ggccctgcag gaggaggagg
      841 agctgcagct ggccctggcg ctgtcacagt cagaggcgga ggagaaggag aggctgagac
      901 agaagtccac gtacacttcg taccccaagg cggagcccat gccctcggcc tcctcagcgc
      961 cccccgccag cagcctgtac tcttcacctg tgaactcgtc ggcgcctctg gctgaggaca
     1021 tcgaccctga gctcgcacgg tatctcaacc ggaactactg ggagaagaag caggaggagg
     1081 ctcgcaagag ccccacgcca tctgcgcccg tgcccctgac ggagccggct gcacagcctg
     1141 gggaagggca cgcagccccc accaacgtgg tggagaaccc cctcccggag acagactctc
     1201 agcccattcc tccctctggt ggccccttta gtgagccaca gttccacaat ggcgagtctg
     1261 aggagagcca cgagcagttc ctgaaggcgc tgcagaacgc cgtcaccacc ttcgtgaacc
     1321 gcatgaagag taaccacatg cggggccgca gcatcaccaa tgactcggcc gtgctctcac
     1381 tcttccagtc catcaacggc atgcacccgc agctgctgga gctgctcaac cagctggacg
     1441 agcgcaggct gtactatgag gggctgcagg acaagctggc acagatccgc gatgcccggg
     1501 gggcgctgag tgccctgcgc gaagagcacc gggagaagct tcgccgggca gccgaggagg
     1561 cagagcgcca gcgccagatc cagctggccc agaagctgga gataatgcgg cagaagaagc
     1621 aggagtacct ggaggtgcag aggcagctgg ccatccagcg cctgcaggag caggagaagg
     1681 agcggcagat gcggctggag cagcagaagc agacggtcca gatgcgcgcg cagatgcccg
     1741 ccttccccct gccctacgcc cagctccagg ccatgcccgc agccggaggt gtgctctacc
     1801 agccctcggg accagccagc ttccccagca ccttcagccc tgccggctcg gtggagggct
     1861 ccccaatgca cggcgtgtac atgagccagc cggcccctgc cgctggcccc taccccagca
     1921 tgcccagcac tgcggctgat cccagcatgg tgagtgccta catgtaccca gcaggggcca
     1981 ctggggcgca ggcggccccc caggcccagg ccggacccac cgccagcccc gcttactcat
     2041 cctaccagcc tactcccaca gcgggctacc agaacgtggc ctcccaggcc ccacagagcc
     2101 tcccggccat ctctcagcct ccgcagtcca gcaccatggg ctacatgggg agccagtcag
     2161 tctccatggg ctaccagcct tacaacatgc agaatctcat gaccaccctc ccaagccagg
     2221 atgcgtctct gccaccccag cagccctaca tcgcggggca gcagcccatg taccagcaga
     2281 tggcaccctc tggcggtccc ccccagcagc agccccccgt ggcccagcaa ccgcaggcac
     2341 aggggccgcc ggcacagggc agcgaggccc agctcatttc attcgactga cccaggccat
     2401 gctcacgtcc ggagtaacac tacatacagt tcacctgaaa cgcctcgtct ctaactgccg
     2461 tcgtcctgcc tccctgtcct ctactgccgg tagtgtccct tctctgcgag tgaggggggg
     2521 ccttcacccc aagcccacct cccttgtcct cagcctactg cagtccctga gttagtctct
     2581 gctttctttc cccagggctg ggccatgggg agggaaggac tttctcccag gggaagcccc
     2641 cagccctgtg ggtcatggtc tgtgagaggt ggcaggaatg gggaccctca ccccccaagc
     2701 agcctgtgcc ctctggccgc actgtgagct ggctgtggtg tctgggtgtg gcctggggct
     2761 ccctctgcag gggcctctct cggcagccac agccaagggt ggaggcttca ggtctccagc
     2821 ttctctgctt ctcagctgcc atctccagtg ccccagaatg gtacagcgat aataaaatgt
     2881 atttcagaaa gca
//
