LOCUS       NM_001528               2069 bp    mRNA    linear   PRI 05-DEC-2024
DEFINITION  Homo sapiens HGF activator (HGFAC), transcript variant 2, mRNA.
ACCESSION   NM_001528
VERSION     NM_001528.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Zou,Q. and Cao,S.
  TITLE     miR-4270 suppresses hepatocellular carcinoma progression by
            inhibiting DNMT3A-mediated methylation of HGFAC promoter
  JOURNAL   PeerJ 11, e16566 (2023)
   PUBMED   38077422
  REMARK    GeneRIF: miR-4270 suppresses hepatocellular carcinoma progression
            by inhibiting DNMT3A-mediated methylation of HGFAC promoter.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Sargsyan,A., Doridot,L., Hannou,S.A., Tong,W., Srinivasan,H.,
            Ivison,R., Monn,R., Kou,H.H., Haldeman,J.M., Arlotto,M.,
            White,P.J., Grimsrud,P.A., Astapova,I., Tsai,L.T. and Herman,M.A.
  TITLE     HGFAC is a ChREBP-regulated hepatokine that enhances glucose and
            lipid homeostasis
  JOURNAL   JCI Insight 8 (1), e153740 (2023)
   PUBMED   36413406
  REMARK    GeneRIF: HGFAC is a ChREBP-regulated hepatokine that enhances
            glucose and lipid homeostasis.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2069)
  AUTHORS   Tokarski,M., Cierzniak,A. and Baczynska,D.
  TITLE     Role of hypoxia on microRNA-dependant regulation of HGFA - HGF -
            c-Met signalling pathway in human progenitor and mature endothelial
            cells
  JOURNAL   Int J Biochem Cell Biol 152, 106310 (2022)
   PUBMED   36182093
  REMARK    GeneRIF: Role of hypoxia on microRNA-dependant regulation of HGFA -
            HGF - c-Met signalling pathway in human progenitor and mature
            endothelial cells.
REFERENCE   4  (bases 1 to 2069)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 2069)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 2069)
  AUTHORS   Miyazawa,K., Shimomura,T. and Kitamura,N.
  TITLE     Activation of hepatocyte growth factor in the injured tissues is
            mediated by hepatocyte growth factor activator
  JOURNAL   J Biol Chem 271 (7), 3615-3618 (1996)
   PUBMED   8631970
REFERENCE   7  (bases 1 to 2069)
  AUTHORS   Shimomura,T., Kondo,J., Ochiai,M., Naka,D., Miyazawa,K.,
            Morimoto,Y. and Kitamura,N.
  TITLE     Activation of the zymogen of hepatocyte growth factor activator by
            thrombin
  JOURNAL   J Biol Chem 268 (30), 22927-22932 (1993)
   PUBMED   8226803
REFERENCE   8  (bases 1 to 2069)
  AUTHORS   Miyazawa,K., Shimomura,T., Kitamura,A., Kondo,J., Morimoto,Y. and
            Kitamura,N.
  TITLE     Molecular cloning and sequence analysis of the cDNA for a human
            serine protease reponsible for activation of hepatocyte growth
            factor. Structural similarity of the protease precursor to blood
            coagulation factor XII
  JOURNAL   J Biol Chem 268 (14), 10024-10028 (1993)
   PUBMED   7683665
REFERENCE   9  (bases 1 to 2069)
  AUTHORS   Shimomura,T., Ochiai,M., Kondo,J. and Morimoto,Y.
  TITLE     A novel protease obtained from FBS-containing culture supernatant,
            that processes single chain form hepatocyte growth factor to two
            chain form in serum-free culture
  JOURNAL   Cytotechnology 8 (3), 219-229 (1992)
   PUBMED   1368819
REFERENCE   10 (bases 1 to 2069)
  AUTHORS   Nakamura,T.
  TITLE     Structure and function of hepatocyte growth factor
  JOURNAL   Prog Growth Factor Res 3 (1), 67-85 (1991)
   PUBMED   1838014
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC112190.1 and D14012.1.
            
            On Jun 2, 2019 this sequence version replaced NM_001528.3.
            
            Summary: This gene encodes a member of the peptidase S1 protein
            family. The encoded protein is first synthesized as an inactive
            single-chain precursor before being activated to a heterodimeric
            form by endoproteolytic processing. It acts as serine protease that
            converts hepatocyte growth factor to the active form. Alternative
            splicing results in multiple transcript variants. [provided by
            RefSeq, Jul 2014].
            
            Transcript Variant: This variant (2) lacks an in-frame exon in the
            central coding region, compared to variant 1. The encoded isoform
            (2) is shorter, compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: D14012.1, BC112190.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2145122, SAMEA2155590
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000382774.8/ ENSP00000372224.4
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-31                BC112190.1         36-66
            32-2067             D14012.1           1-2036
            2068-2069           BC112190.1         2103-2104
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4p16.3"
     gene            1..2069
                     /gene="HGFAC"
                     /gene_synonym="HGFA"
                     /note="HGF activator"
                     /db_xref="GeneID:3083"
                     /db_xref="HGNC:HGNC:4894"
                     /db_xref="MIM:604552"
     exon            1..151
                     /gene="HGFAC"
                     /gene_synonym="HGFA"
                     /inference="alignment:Splign:2.1.0"
     CDS             35..2002
                     /gene="HGFAC"
                     /gene_synonym="HGFA"
                     /EC_number="3.4.21.-"
                     /note="isoform 2 preproprotein is encoded by transcript
                     variant 2; hepatocyte growth factor activator"
                     /codon_start=1
                     /product="hepatocyte growth factor activator isoform 2
                     preproprotein"
                     /protein_id="NP_001519.1"
                     /db_xref="CCDS:CCDS3369.1"
                     /db_xref="GeneID:3083"
                     /db_xref="HGNC:HGNC:4894"
                     /db_xref="MIM:604552"
                     /translation="MGRWAWVPSPWPPPGLGPFLLLLLLLLLLPRGFQPQPGGNRTES
                     PEPNATATPAIPTILVTSVTSETPATSAPEAEGPQSGGLPPPPRAVPSSSSPQAQALT
                     EDGRPCRFPFRYGGRMLHACTSEGSAHRKWCATTHNYDRDRAWGYCVEATPPPGGPAA
                     LDPCASGPCLNGGSCSNTQDPQSYHCSCPRAFTGKDCGTEKCFDETRYEYLEGGDRWA
                     RVRQGHVEQCECFGGRTWCEGTRHTACLSSPCLNGGTCHLIVATGTTVCACPPGFAGR
                     LCNIEPDERCFLGNGTGYRGVASTSASGLSCLAWNSDLLYQELHVDSVGAAALLGLGP
                     HAYCRNPDNDERPWCYVVKDSALSWEYCRLEACESLTRVQLSPDLLATLPEPASPGRQ
                     ACGRRHKKRTFLRPRIIGGSSSLPGSHPWLAAIYIGDSFCAGSLVHTCWVVSAAHCFS
                     HSPPRDSVSVVLGQHFFNRTTDVTQTFGIEKYIPYTLYSVFNPSDHDLVLIRLKKKGD
                     RCATRSQFVQPICLPEPGSTFPAGHKCQIAGWGHLDENVSGYSSSLREALVPLVADHK
                     CSSPEVYGADISPNMLCAGYFDCKSDACQGDSGGPLACEKNGVAYLYGIISWGDGCGR
                     LHKPGVYTRVANYVDWINDRIRPPRRLVAPS"
     exon            152..332
                     /gene="HGFAC"
                     /gene_synonym="HGFA"
                     /inference="alignment:Splign:2.1.0"
     exon            333..429
                     /gene="HGFAC"
                     /gene_synonym="HGFA"
                     /inference="alignment:Splign:2.1.0"
     exon            430..509
                     /gene="HGFAC"
                     /gene_synonym="HGFA"
                     /inference="alignment:Splign:2.1.0"
     exon            510..632
                     /gene="HGFAC"
                     /gene_synonym="HGFA"
                     /inference="alignment:Splign:2.1.0"
     exon            633..764
                     /gene="HGFAC"
                     /gene_synonym="HGFA"
                     /inference="alignment:Splign:2.1.0"
     exon            765..875
                     /gene="HGFAC"
                     /gene_synonym="HGFA"
                     /inference="alignment:Splign:2.1.0"
     exon            876..1050
                     /gene="HGFAC"
                     /gene_synonym="HGFA"
                     /inference="alignment:Splign:2.1.0"
     exon            1051..1136
                     /gene="HGFAC"
                     /gene_synonym="HGFA"
                     /inference="alignment:Splign:2.1.0"
     exon            1137..1389
                     /gene="HGFAC"
                     /gene_synonym="HGFA"
                     /inference="alignment:Splign:2.1.0"
     exon            1390..1529
                     /gene="HGFAC"
                     /gene_synonym="HGFA"
                     /inference="alignment:Splign:2.1.0"
     exon            1530..1670
                     /gene="HGFAC"
                     /gene_synonym="HGFA"
                     /inference="alignment:Splign:2.1.0"
     exon            1671..1819
                     /gene="HGFAC"
                     /gene_synonym="HGFA"
                     /inference="alignment:Splign:2.1.0"
     exon            1820..2069
                     /gene="HGFAC"
                     /gene_synonym="HGFA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2050..2055
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HGFAC"
                     /gene_synonym="HGFA"
                     /note="hexamer: AATAAA"
     polyA_site      2069
                     /gene="HGFAC"
                     /gene_synonym="HGFA"
                     /note="major polyA site"
ORIGIN      
        1 gcctcccact gcccctcagg ccagctcagg agccatgggg cgctgggcct gggtccccag
       61 cccctggccc ccaccggggc tgggcccctt cctcctcctc ctcctgctgc tgctgctgct
      121 gccacggggg ttccagcccc agcctggcgg gaaccgtacg gagtccccag aacctaatgc
      181 cacagcgacc cctgcgatcc ccactatcct ggtgacctct gtgacctctg agaccccagc
      241 aacaagtgct ccagaggcag agggacccca aagtgggggg ctcccgcccc cgcccagggc
      301 agttccctcg agcagtagcc cccaggccca agcactcacc gaggacggga ggccctgcag
      361 gttccccttc cgctacgggg gccgcatgct gcatgcctgc acttcggagg gcagtgcaca
      421 caggaagtgg tgtgccacaa ctcacaacta cgaccgggac agggcctggg gctactgtgt
      481 ggaggccacc ccgcctccag ggggcccagc tgccctggat ccctgtgcct ccggcccctg
      541 cctcaatgga ggctcctgct ccaataccca ggacccccag tcctatcact gcagctgccc
      601 ccgggccttc accggcaagg actgcggcac agagaaatgc tttgatgaga cccgctacga
      661 gtacctggag gggggcgacc gctgggcccg cgtgcgccag ggccacgtgg aacagtgcga
      721 gtgcttcggg ggccggacct ggtgcgaagg cacccgacat acagcttgtc tgagcagccc
      781 ttgcctgaac gggggcacct gccacctgat cgtggccacc gggaccaccg tgtgtgcctg
      841 cccaccaggc ttcgctggac ggctctgcaa catcgagcct gatgagcgct gcttcttggg
      901 gaacggcact gggtaccgtg gcgtggccag cacctcagcc tcgggcctca gctgcctggc
      961 ctggaactcc gatctgctct accaggagct gcacgtggac tccgtgggcg ccgcggccct
     1021 gctgggcctg ggcccccatg cctactgccg gaatccggac aatgacgaga ggccctggtg
     1081 ctacgtggtg aaggacagcg cgctctcctg ggagtactgc cgcctggagg cctgcgaatc
     1141 cctcaccaga gtccaactgt caccggatct cctggcgacc ctgcctgagc cagcctcccc
     1201 ggggcgccag gcctgcggca ggaggcacaa gaagaggacg ttcctgcggc cacgtatcat
     1261 cggcggctcc tcctcgctgc ccggctcgca cccctggctg gccgccatct acatcgggga
     1321 cagcttctgc gccgggagcc tggtccacac ctgctgggtg gtgtcggccg cccactgctt
     1381 ctcccacagc ccccccaggg acagcgtctc cgtggtgctg ggccagcact tcttcaaccg
     1441 cacgacggac gtgacgcaga ccttcggcat cgagaagtac atcccgtaca ccctgtactc
     1501 ggtgttcaac cccagcgacc acgacctcgt cctgatccgg ctgaagaaga aaggggaccg
     1561 ctgtgccaca cgctcgcagt tcgtgcagcc catctgcctg cccgagcccg gcagcacctt
     1621 ccccgcagga cacaagtgcc agattgcggg ctggggccac ttggatgaga acgtgagcgg
     1681 ctactccagc tccctgcggg aggccctggt ccccctggtc gccgaccaca agtgcagcag
     1741 ccctgaggtc tacggcgccg acatcagccc caacatgctc tgtgccggct acttcgactg
     1801 caagtccgac gcctgccagg gggactcagg ggggcccctg gcctgcgaga agaacggcgt
     1861 ggcttacctc tacggcatca tcagctgggg tgacggctgc gggcggctcc acaagccggg
     1921 ggtctacacc cgcgtggcca actatgtgga ctggatcaac gaccggatac ggcctcccag
     1981 gcggcttgtg gctccctcct gaccctccag cgggacaccc tggttcccac cattccctgc
     2041 cttgctgaca ataaagatat ttccaagaa
//
