LOCUS       NM_021170                885 bp    mRNA    linear   PRI 01-JUN-2024
DEFINITION  Homo sapiens hes family bHLH transcription factor 4 (HES4),
            transcript variant 2, mRNA.
ACCESSION   NM_021170
VERSION     NM_021170.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 885)
  AUTHORS   De Decker,M., Lavaert,M., Roels,J., Tilleman,L., Vandekerckhove,B.,
            Leclercq,G., Van Nieuwerburgh,F., Van Vlierberghe,P. and Taghon,T.
  TITLE     HES1 and HES4 have non-redundant roles downstream of Notch during
            early human T-cell development
  JOURNAL   Haematologica 106 (1), 130-141 (2021)
   PUBMED   31919081
  REMARK    GeneRIF: HES1 and HES4 have non-redundant roles downstream of Notch
            during early human T-cell development.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 885)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   3  (bases 1 to 885)
  AUTHORS   McManus,M., Kleinerman,E., Yang,Y., Livingston,J.A., Mortus,J.,
            Rivera,R., Zweidler-McKay,P. and Schadler,K.
  TITLE     Hes4: A potential prognostic biomarker for newly diagnosed patients
            with high-grade osteosarcoma
  JOURNAL   Pediatr Blood Cancer 64 (5) (2017)
   PUBMED   27786411
  REMARK    GeneRIF: Hes4 overexpression promotes a more aggressive tumor
            phenotype by preventing osteoblastic differentiation of
            osteosarcoma cells. Hes4 expression may allow for the
            stratification of patients into good or poor responders to
            chemotherapy at diagnosis.
REFERENCE   4  (bases 1 to 885)
  AUTHORS   Cakouros,D., Isenmann,S., Hemming,S.E., Menicanin,D., Camp,E.,
            Zannetinno,A.C. and Gronthos,S.
  TITLE     Novel basic helix-loop-helix transcription factor hes4 antagonizes
            the function of twist-1 to regulate lineage commitment of bone
            marrow stromal/stem cells
  JOURNAL   Stem Cells Dev 24 (11), 1297-1308 (2015)
   PUBMED   25579220
  REMARK    GeneRIF: Overexpression and knockdown studies demonstrated that
            Hes4 promotes osteogenesis resulting in an increase in Runx2,
            osteocalcin, osteopontin, and bone sialoprotein expression in BMSC.
REFERENCE   5  (bases 1 to 885)
  AUTHORS   Bai,G., Cheung,I., Shulha,H.P., Coelho,J.E., Li,P., Dong,X.,
            Jakovcevski,M., Wang,Y., Grigorenko,A., Jiang,Y., Hoss,A.,
            Patel,K., Zheng,M., Rogaev,E., Myers,R.H., Weng,Z., Akbarian,S. and
            Chen,J.F.
  TITLE     Epigenetic dysregulation of hairy and enhancer of split 4 (HES4) is
            associated with striatal degeneration in postmortem Huntington
            brains
  JOURNAL   Hum Mol Genet 24 (5), 1441-1456 (2015)
   PUBMED   25480889
  REMARK    GeneRIF: Epigenetic dysregulation of HES4 could play a critical
            role in modifying Huntington's disease pathogenesis and severity.
REFERENCE   6  (bases 1 to 885)
  AUTHORS   Reina Ortiz,M., Schreiber,F., Benitez,S., Broncano,N., Chico,M.E.,
            Vaca,M., Alexander,N., Lewis,D.J., Dougan,G. and Cooper,P.J.
  TITLE     Effects of chronic ascariasis and trichuriasis on cytokine
            production and gene expression in human blood: a cross-sectional
            study
  JOURNAL   PLoS Negl Trop Dis 5 (6), e1157 (2011)
   PUBMED   21666788
REFERENCE   7  (bases 1 to 885)
  AUTHORS   Vaquerizas,J.M., Kummerfeld,S.K., Teichmann,S.A. and Luscombe,N.M.
  TITLE     A census of human transcription factors: function, expression and
            evolution
  JOURNAL   Nat Rev Genet 10 (4), 252-263 (2009)
   PUBMED   19274049
  REMARK    Review article
REFERENCE   8  (bases 1 to 885)
  AUTHORS   Katoh,M. and Katoh,M.
  TITLE     Integrative genomic analyses on HES/HEY family: Notch-independent
            HES1, HES3 transcription in undifferentiated ES cells, and
            Notch-dependent HES1, HES5, HEY1, HEY2, HEYL transcription in fetal
            tissues, adult tissues, or cancer
  JOURNAL   Int J Oncol 31 (2), 461-466 (2007)
   PUBMED   17611704
REFERENCE   9  (bases 1 to 885)
  AUTHORS   Katoh,M. and Katoh,M.
  TITLE     Identification and characterization of human HES2, HES3, and HES5
            genes in silico
  JOURNAL   Int J Oncol 25 (2), 529-534 (2004)
   PUBMED   15254753
REFERENCE   10 (bases 1 to 885)
  AUTHORS   Bessho,Y., Miyoshi,G., Sakata,R. and Kageyama,R.
  TITLE     Hes7: a bHLH-type repressor gene regulated by Notch and expressed
            in the presomitic mesoderm
  JOURNAL   Genes Cells 6 (2), 175-185 (2001)
   PUBMED   11260262
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BI757407.1, BC012351.1 and DB485006.1.
            
            On Jun 2, 2019 this sequence version replaced NM_021170.3.
            
            Transcript Variant: This variant (2) represents an alternate intron
            and encodes a shorter protein (isoform 2), compared to transcript
            variant 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC012351.1, BU622703.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2144333 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000304952.11/ ENSP00000304595.7
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-7                 BI757407.1         76-82
            8-18                BC012351.1         1-11
            19-232              DB485006.1         17-230
            233-885             BC012351.1         226-878
FEATURES             Location/Qualifiers
     source          1..885
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p36.33"
     gene            1..885
                     /gene="HES4"
                     /gene_synonym="bHLHb42"
                     /note="hes family bHLH transcription factor 4"
                     /db_xref="GeneID:57801"
                     /db_xref="HGNC:HGNC:24149"
                     /db_xref="MIM:608060"
     exon            1..232
                     /gene="HES4"
                     /gene_synonym="bHLHb42"
                     /inference="alignment:Splign:2.1.0"
     CDS             125..790
                     /gene="HES4"
                     /gene_synonym="bHLHb42"
                     /note="isoform 2 is encoded by transcript variant 2; bHLH
                     factor Hes4; transcription factor HES-4; hHES4; class B
                     basic helix-loop-helix protein 42; hairy and enhancer of
                     split 4"
                     /codon_start=1
                     /product="transcription factor HES-4 isoform 2"
                     /protein_id="NP_066993.1"
                     /db_xref="CCDS:CCDS5.1"
                     /db_xref="GeneID:57801"
                     /db_xref="HGNC:HGNC:24149"
                     /db_xref="MIM:608060"
                     /translation="MAADTPGKPSASPMAGAPASASRTPDKPRSAAEHRKSSKPVMEK
                     RRRARINESLAQLKTLILDALRKESSRHSKLEKADILEMTVRHLRSLRRVQVTAALSA
                     DPAVLGKYRAGFHECLAEVNRFLAGCEGVPADVRSRLLGHLAACLRQLGPSRRPASLS
                     PAAPAEAPAPEVYAGRPLLPSLGGPFPLLAPPLLPGLTRALPAAPRAGPQGPGGPWRP
                     WLR"
     exon            233..328
                     /gene="HES4"
                     /gene_synonym="bHLHb42"
                     /inference="alignment:Splign:2.1.0"
     exon            329..416
                     /gene="HES4"
                     /gene_synonym="bHLHb42"
                     /inference="alignment:Splign:2.1.0"
     exon            417..885
                     /gene="HES4"
                     /gene_synonym="bHLHb42"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      885
                     /gene="HES4"
                     /gene_synonym="bHLHb42"
                     /note="major polyA site"
ORIGIN      
        1 gcgggcctgg agccgggatc cgccctaggg gctcggatcg ccgcgcgctc gccgctcgcc
       61 cgccagcccg cccgtggtcc gtggcggcgc gctccacccg gcacggggag gcgcggggcg
      121 caccatggcc gcagacacgc cggggaaacc gagcgcctcg ccgatggcag gagcgccggc
      181 cagcgccagc cggaccccag acaagccccg gagcgcggcc gagcaccgca agtcctccaa
      241 gccggtcatg gagaagcggc gccgagcgcg tattaacgag agcctcgctc agctcaaaac
      301 cctcatcctg gacgccctca gaaaagagag ctcccgccac tcgaagctgg agaaggcgga
      361 catcctggag atgaccgtga gacacctgcg gagcctgcgt cgcgtgcagg tgacggccgc
      421 gctcagcgcc gaccccgccg ttctgggcaa gtaccgcgcc ggcttccacg agtgtctggc
      481 ggaggtgaac cgcttcctgg ccggctgcga gggcgtcccg gccgacgtgc gctcccgcct
      541 gctgggccac ctggcagcct gcctgcgcca gctgggaccc tcccgccgcc cggcctcgct
      601 gtccccggct gcccccgcag aggccccagc gcccgaggtc tacgcgggcc gcccgctgct
      661 gccatcgctc ggcggcccct tccctctgct cgcgccgccg ctgctgccgg gtctgacccg
      721 ggcgctgccc gccgccccca gggcggggcc gcagggcccg ggtgggccct ggaggccgtg
      781 gctgcgctga ggctgtggcc ctgagactgc atcggaggcg gcgccccgtt ctagggccgt
      841 ggcctttgcc gagactgtag cagagaaaac gtatttatta ttcca
//
