LOCUS       NM_016323               3511 bp    mRNA    linear   PRI 02-MAY-2024
DEFINITION  Homo sapiens HECT and RLD domain containing E3 ubiquitin protein
            ligase 5 (HERC5), mRNA.
ACCESSION   NM_016323
VERSION     NM_016323.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Schneegans,S., Loptien,J., Mojzisch,A., Loreth,D., Kretz,O.,
            Raschdorf,C., Hanssen,A., Gocke,A., Siebels,B., Gunasekaran,K.,
            Ding,Y., Oliveira-Ferrer,L., Brylka,L., Schinke,T., Schluter,H.,
            Paatero,I., Voss,H., Werner,S., Pantel,K. and Wikman,H.
  TITLE     HERC5 downregulation in non-small cell lung cancer is associated
            with altered energy metabolism and metastasis
  JOURNAL   J Exp Clin Cancer Res 43 (1), 110 (2024)
   PUBMED   38605423
  REMARK    GeneRIF: HERC5 downregulation in non-small cell lung cancer is
            associated with altered energy metabolism and metastasis.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Qin,Y., Meng,X., Wang,M., Liang,W., Xu,R., Chen,J., Song,H., Fu,Y.,
            Li,J., Gao,C., Jia,M., Zhao,C. and Zhao,W.
  TITLE     Posttranslational ISGylation of NLRP3 by HERC enzymes facilitates
            inflammasome activation in models of inflammation
  JOURNAL   J Clin Invest 133 (20), e161935 (2023)
   PUBMED   37651190
  REMARK    GeneRIF: Posttranslational ISGylation of NLRP3 by HERC enzymes
            facilitates inflammasome activation in models of inflammation.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3511)
  AUTHORS   Du,X., Sheng,J., Chen,Y., He,S., Yang,Y., Huang,Y., Fu,Y., Lie,L.,
            Han,Z., Zhu,B., Liu,H., Wen,Q., Zhou,X., Zhou,C., Hu,S. and Ma,L.
  TITLE     The E3 ligase HERC5 promotes antimycobacterial responses in
            macrophages by ISGylating the phosphatase PTEN
  JOURNAL   Sci Signal 16 (788), eabm1756 (2023)
   PUBMED   37279284
  REMARK    GeneRIF: The E3 ligase HERC5 promotes antimycobacterial responses
            in macrophages by ISGylating the phosphatase PTEN.
REFERENCE   4  (bases 1 to 3511)
  AUTHORS   Liu,C., Li,L., Hou,G., Lu,Y., Gao,M. and Zhang,L.
  TITLE     HERC5/IFI16/p53 signaling mediates breast cancer cell proliferation
            and migration
  JOURNAL   Life Sci 303, 120692 (2022)
   PUBMED   35671810
  REMARK    GeneRIF: HERC5/IFI16/p53 signaling mediates breast cancer cell
            proliferation and migration.
REFERENCE   5  (bases 1 to 3511)
  AUTHORS   Bawono,R.G., Abe,T., Qu,M., Kuroki,D., Deng,L., Matsui,C., Ryo,A.,
            Suzuki,T., Matsuura,Y., Sugiyama,M., Mizokami,M., Shimotohno,K. and
            Shoji,I.
  TITLE     HERC5 E3 ligase mediates ISGylation of hepatitis B virus X protein
            to promote viral replication
  JOURNAL   J Gen Virol 102 (10) (2021)
   PUBMED   34661519
  REMARK    GeneRIF: HERC5 E3 ligase mediates ISGylation of hepatitis B virus X
            protein to promote viral replication.
REFERENCE   6  (bases 1 to 3511)
  AUTHORS   Wong,J.J., Pung,Y.F., Sze,N.S. and Chin,K.C.
  TITLE     HERC5 is an IFN-induced HECT-type E3 protein ligase that mediates
            type I IFN-induced ISGylation of protein targets
  JOURNAL   Proc Natl Acad Sci U S A 103 (28), 10735-10740 (2006)
   PUBMED   16815975
  REMARK    GeneRIF: HERC5/Ceb1 is involved in the conjugation of ISG15 to
            cellular proteins.
REFERENCE   7  (bases 1 to 3511)
  AUTHORS   Dastur,A., Beaudenon,S., Kelley,M., Krug,R.M. and Huibregtse,J.M.
  TITLE     Herc5, an interferon-induced HECT E3 enzyme, is required for
            conjugation of ISG15 in human cells
  JOURNAL   J Biol Chem 281 (7), 4334-4338 (2006)
   PUBMED   16407192
REFERENCE   8  (bases 1 to 3511)
  AUTHORS   Hochrainer,K., Mayer,H., Baranyi,U., Binder,B., Lipp,J. and
            Kroismayr,R.
  TITLE     The human HERC family of ubiquitin ligases: novel members, genomic
            organization, expression profiling, and evolutionary aspects
  JOURNAL   Genomics 85 (2), 153-164 (2005)
   PUBMED   15676274
REFERENCE   9  (bases 1 to 3511)
  AUTHORS   Kroismayr,R., Baranyi,U., Stehlik,C., Dorfleutner,A., Binder,B.R.
            and Lipp,J.
  TITLE     HERC5, a HECT E3 ubiquitin ligase tightly regulated in LPS
            activated endothelial cells
  JOURNAL   J Cell Sci 117 (Pt 20), 4749-4756 (2004)
   PUBMED   15331633
  REMARK    GeneRIF: Data show that HERC5, a functionally active HECT ubiquitin
            ligase, exhibits a tightly controlled cytosolic level under
            inflammatory conditions in endothelial cells.
REFERENCE   10 (bases 1 to 3511)
  AUTHORS   Mitsui,K., Nakanishi,M., Ohtsuka,S., Norwood,T.H., Okabayashi,K.,
            Miyamoto,C., Tanaka,K., Yoshimura,A. and Ohtsubo,M.
  TITLE     A novel human gene encoding HECT domain and RCC1-like repeats
            interacts with cyclins and is potentially regulated by the tumor
            suppressor proteins
  JOURNAL   Biochem Biophys Res Commun 266 (1), 115-122 (1999)
   PUBMED   10581175
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AY337518.1, BC140716.1,
            AK302142.1, AC083829.5 and AI336946.1.
            
            On Nov 22, 2018 this sequence version replaced NM_016323.3.
            
            Summary: This gene is a member of the HERC family of ubiquitin
            ligases and encodes a protein with a HECT domain and five RCC1
            repeats. Pro-inflammatory cytokines upregulate expression of this
            gene in endothelial cells. The protein localizes to the cytoplasm
            and perinuclear region and functions as an interferon-induced E3
            protein ligase that mediates ISGylation of protein targets. The
            protein also acts as a modulator of the antiviral immune response.
            The gene lies in a cluster of HERC family genes on chromosome 4.
            [provided by RefSeq, Aug 2021].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803616.122484.1, AY337518.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            coronavirus related    :: involved in immune response or antiviral
                                      activity
            MANE Ensembl match     :: ENST00000264350.8/ ENSP00000264350.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-761               AY337518.1         3-763
            762-1642            BC140716.1         657-1537
            1643-2508           AK302142.1         517-1382
            2509-2509           AC083829.5         120842-120842
            2510-3340           BC140716.1         2405-3235
            3341-3511           AI336946.1         29-199              c
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q22.1"
     gene            1..3511
                     /gene="HERC5"
                     /gene_synonym="CEB1; CEBP1"
                     /note="HECT and RLD domain containing E3 ubiquitin protein
                     ligase 5"
                     /db_xref="GeneID:51191"
                     /db_xref="HGNC:HGNC:24368"
                     /db_xref="MIM:608242"
     exon            1..416
                     /gene="HERC5"
                     /gene_synonym="CEB1; CEBP1"
                     /inference="alignment:Splign:2.1.0"
     CDS             152..3226
                     /gene="HERC5"
                     /gene_synonym="CEB1; CEBP1"
                     /note="probable E3 ubiquitin-protein ligase HERC5; HECT
                     domain and RCC1-like domain-containing protein 5;
                     cyclin-E-binding protein 1; hect domain and RLD 5"
                     /codon_start=1
                     /product="E3 ISG15--protein ligase HERC5"
                     /protein_id="NP_057407.2"
                     /db_xref="CCDS:CCDS3630.1"
                     /db_xref="GeneID:51191"
                     /db_xref="HGNC:HGNC:24368"
                     /db_xref="MIM:608242"
                     /translation="MERRSRRKSRRNGRSTAGKAAATQPAKSPGAQLWLFPSAAGLHR
                     ALLRRVEVTRQLCCSPGRLAVLERGGAGVQVHQLLAGSGGARTPKCIKLGKNMKIHSV
                     DQGAEHMLILSSDGKPFEYDNYSMKHLRFESILQEKKIIQITCGDYHSLALSKGGELF
                     AWGQNLHGQLGVGRKFPSTTTPQIVEHLAGVPLAQISAGEAHSMALSMSGNIYSWGKN
                     ECGQLGLGHTESKDDPSLIEGLDNQKVEFVACGGSHSALLTQDGLLFTFGAGKHGQLG
                     HNSTQNELRPCLVAELVGYRVTQIACGRWHTLAYVSDLGKVFSFGSGKDGQLGNGGTR
                     DQLMPLPVKVSSSEELKLESHTSEKELIMIAGGNQSILLWIKKENSYVNLKRTIPTLN
                     EGTVKRWIADVETKRWQSTKREIQEIFSSPACLTGSFLRKRRTTEMMPVYLDLNKARN
                     IFKELTQKDWITNMITTCLKDNLLKRLPFHSPPQEALEIFFLLPECPMMHISNNWESL
                     VVPFAKVVCKMSDQSSLVLEEYWATLQESTFSKLVQMFKTAVICQLDYWDESAEENGN
                     VQALLEMLKKLHRVNQVKCQLPESIFQVDELLHRLNFFVEVCRRYLWKMTVDASENVQ
                     CCVIFSHFPFIFNNLSKIKLLHTDTLLKIESKKHKAYLRSAAIEEERESEFALRPTFD
                     LTVRRNHLIEDVLNQLSQFENEDLRKELWVSFSGEIGYDLGGVKKEFFYCLFAEMIQP
                     EYGMFMYPEGASCMWFPVKPKFEKKRYFFFGVLCGLSLFNCNVANLPFPLALFKKLLD
                     QMPSLEDLKELSPDLGKNLQTLLDDEGDNFEEVFYIHFNVHWDRNDTNLIPNGSSITV
                     NQTNKRDYVSKYINYIFNDSVKAVYEEFRRGFYKMCDEDIIKLFHPEELKDVIVGNTD
                     YDWKTFEKNARYEPGYNSSHPTIVMFWKAFHKLTLEEKKKFLVFLTGTDRLQMKDLNN
                     MKITFCCPESWNERDPIRALTCFSVLFLPKYSTMETVEEALQEAINNNRGFG"
     exon            417..540
                     /gene="HERC5"
                     /gene_synonym="CEB1; CEBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            541..617
                     /gene="HERC5"
                     /gene_synonym="CEB1; CEBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            618..839
                     /gene="HERC5"
                     /gene_synonym="CEB1; CEBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            840..931
                     /gene="HERC5"
                     /gene_synonym="CEB1; CEBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            932..1062
                     /gene="HERC5"
                     /gene_synonym="CEB1; CEBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1063..1208
                     /gene="HERC5"
                     /gene_synonym="CEB1; CEBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1209..1285
                     /gene="HERC5"
                     /gene_synonym="CEB1; CEBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1286..1389
                     /gene="HERC5"
                     /gene_synonym="CEB1; CEBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1390..1449
                     /gene="HERC5"
                     /gene_synonym="CEB1; CEBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1450..1543
                     /gene="HERC5"
                     /gene_synonym="CEB1; CEBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1544..1733
                     /gene="HERC5"
                     /gene_synonym="CEB1; CEBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1734..1888
                     /gene="HERC5"
                     /gene_synonym="CEB1; CEBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1889..2002
                     /gene="HERC5"
                     /gene_synonym="CEB1; CEBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2003..2113
                     /gene="HERC5"
                     /gene_synonym="CEB1; CEBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2114..2284
                     /gene="HERC5"
                     /gene_synonym="CEB1; CEBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2285..2428
                     /gene="HERC5"
                     /gene_synonym="CEB1; CEBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2429..2595
                     /gene="HERC5"
                     /gene_synonym="CEB1; CEBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2596..2662
                     /gene="HERC5"
                     /gene_synonym="CEB1; CEBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2663..2733
                     /gene="HERC5"
                     /gene_synonym="CEB1; CEBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2734..2917
                     /gene="HERC5"
                     /gene_synonym="CEB1; CEBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2918..3020
                     /gene="HERC5"
                     /gene_synonym="CEB1; CEBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3021..3511
                     /gene="HERC5"
                     /gene_synonym="CEB1; CEBP1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3490..3495
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HERC5"
                     /gene_synonym="CEB1; CEBP1"
                     /note="hexamer: AATAAA"
     polyA_site      3511
                     /gene="HERC5"
                     /gene_synonym="CEB1; CEBP1"
                     /note="major polyA site"
ORIGIN      
        1 agtagctgag gctgcggttc cccgacgcca cgcagctgcg cgcagctggt tcccgctctg
       61 cagcgcaacg cctgaggcag tgggcgcgct cagtcccggg accaggcgtt ctctcctctc
      121 gcctctgggc ctgggacccc gcaaagcggc gatggagcgg aggtcgcgga ggaagtcgcg
      181 gcgcaacggg cgctcgaccg cgggcaaggc cgccgcgacc cagcccgcga agtctccggg
      241 cgcacagctc tggctctttc ccagcgccgc gggcctccac cgcgcgctgc tccggagggt
      301 ggaggtgacg cgccaactct gctgctcgcc ggggcgcctc gcggtcttgg aacgcggcgg
      361 ggcgggcgtc caggttcacc agctgctcgc cgggagcggc ggcgcccgga cgccgaaatg
      421 cattaaatta ggaaaaaaca tgaagataca ttccgtggac caaggagcag agcacatgct
      481 gattctctca tcagatggaa aaccatttga gtatgacaac tatagcatga aacatctaag
      541 gtttgaaagc attttacaag aaaaaaaaat aattcagatc acatgtggag attaccattc
      601 tcttgcactc tcaaaaggtg gtgagctttt tgcctgggga cagaacctgc atgggcagct
      661 tggagttgga aggaaatttc cctcaaccac cacaccacag attgtggagc acctcgcagg
      721 agtacccttg gctcagattt ctgccggaga agcccacagc atggccttat ccatgtctgg
      781 caacatttat tcatggggaa aaaatgaatg tggacaacta ggcctgggcc acactgagag
      841 taaagatgat ccatccctta ttgaaggact agacaatcag aaagttgaat ttgtcgcttg
      901 tggtggctct cacagtgccc tactcacaca ggatgggctg ctgtttactt tcggtgctgg
      961 aaaacatggg caacttggtc ataattcaac acagaatgag ctaagaccct gtttggtggc
     1021 tgagcttgtt gggtatagag tgactcagat agcatgtgga aggtggcaca cacttgccta
     1081 tgtttctgat ttgggaaagg tcttttcctt tggttctgga aaagatggac aactgggaaa
     1141 tggtggaaca cgtgaccagc tgatgccgct tccagtgaaa gtatcatcaa gtgaagaact
     1201 caaacttgaa agccatacct cagaaaagga gttaataatg attgctggag ggaatcaaag
     1261 cattttgctc tggataaaga aagagaattc atatgttaat ctgaagagga caattcctac
     1321 tctgaatgaa gggactgtaa agagatggat tgctgatgtg gagactaaac ggtggcagag
     1381 cacaaaaagg gaaatccaag agatattttc atctcctgct tgtctaactg gaagtttttt
     1441 aaggaaaaga agaactacag aaatgatgcc tgtttatttg gacttaaata aagcaagaaa
     1501 catcttcaag gagttaaccc aaaaggactg gattactaac atgataacca cctgcctcaa
     1561 agataatctg ctcaaaagac ttccatttca ttctccaccc caagaagctt tagaaatttt
     1621 cttccttctc ccagaatgtc ctatgatgca tatttccaac aactgggaga gccttgtggt
     1681 tccatttgca aaggttgttt gtaaaatgag tgaccagtct tcactggttc tggaagagta
     1741 ttgggcaact ctgcaagaat ccactttcag caaactggtc cagatgttta aaacagccgt
     1801 catatgccag ttggattact gggatgaaag tgctgaggag aatggtaatg ttcaagctct
     1861 cctagaaatg ttgaagaagc tgcacagggt aaaccaggtg aaatgtcaac tacctgaaag
     1921 tattttccaa gtagacgaac tcttgcaccg tctcaatttt tttgtagaag tatgcagaag
     1981 gtacttgtgg aaaatgactg tggacgcttc agaaaatgta caatgctgcg tcatattcag
     2041 tcactttcca tttatcttta ataatctgtc gaaaattaaa ctactacata cagacacact
     2101 tttaaaaata gagagtaaaa aacataaagc ttatcttagg tcggcagcaa ttgaggaaga
     2161 aagagagtct gaattcgctt tgaggcccac gtttgatcta acagtcagaa ggaatcactt
     2221 gattgaggat gttttgaatc agctaagtca atttgagaat gaagacctga ggaaagagtt
     2281 atgggtttca tttagtggag aaattgggta tgacctcgga ggagtcaaga aagagttctt
     2341 ctactgtctg tttgcagaga tgatccagcc ggaatatggg atgttcatgt atcctgaagg
     2401 ggcttcctgc atgtggtttc ctgtcaagcc taaatttgag aagaaaagat acttcttttt
     2461 tggggttcta tgtggacttt ccctgttcaa ttgcaatgtt gccaaccttc ctttcccact
     2521 ggcactgttt aagaaacttt tggaccaaat gccatcattg gaagacttga aagaactcag
     2581 tcctgatttg ggaaagaatt tgcaaacact tctggatgat gaaggtgata actttgagga
     2641 agtattttac atccatttta atgtgcactg ggacagaaac gacacaaact taattcctaa
     2701 tggaagtagc ataactgtca accagactaa caagagagac tatgtttcta agtatatcaa
     2761 ttacattttc aacgactctg taaaggcggt ttatgaagaa tttcggagag gattttataa
     2821 aatgtgcgac gaagacatta tcaaattatt ccaccccgaa gaactgaagg atgtgattgt
     2881 tggaaataca gattatgatt ggaaaacatt tgaaaagaat gcacgttatg aaccaggata
     2941 taacagttca catcccacca tagtgatgtt ttggaaggct ttccacaaat tgactctgga
     3001 agaaaagaaa aaattccttg tatttcttac aggaactgac agactacaaa tgaaagattt
     3061 aaataatatg aaaataacat tttgctgtcc tgaaagttgg aatgaaagag accctataag
     3121 agcactgaca tgtttcagtg tcctcttcct ccctaaatat tctacaatgg aaacagttga
     3181 agaagcgctt caagaagcca tcaacaacaa cagaggattt ggctgaccag cttgcttgtc
     3241 caacagcctt attttgttgt tgttatcgtt gttgttgttg ttgttgttgt tgtttctcta
     3301 ctttgttttg ttttaggctt ttagcagcct gaagccatgg tttttcattt ctgtctctag
     3361 tgataagcag gaaagaggga tgaagaagag ggtttactgg ccggttagaa cccgtgactg
     3421 tattctctcc cttggatacc cctatgccta catcatattc cttacctctt ttgggaaata
     3481 tttttcaaaa ataaaataac cgaaaaatta a
//
