LOCUS       NM_015601               4424 bp    mRNA    linear   PRI 06-APR-2024
DEFINITION  Homo sapiens HECT and RLD domain containing E3 ubiquitin protein
            ligase 4 (HERC4), transcript variant 2, mRNA.
ACCESSION   NM_015601
VERSION     NM_015601.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4424)
  AUTHORS   Zhu,Q., Yang,X. and Lv,Y.
  TITLE     HERC4 modulates ovarian cancer cell proliferation by regulating
            SMO-elicited hedgehog signaling
  JOURNAL   Biochim Biophys Acta Gen Subj 1868 (4), 130557 (2024)
   PUBMED   38181892
  REMARK    GeneRIF: HERC4 modulates ovarian cancer cell proliferation by
            regulating SMO-elicited hedgehog signaling.
REFERENCE   2  (bases 1 to 4424)
  AUTHORS   Zhang,Z., Li,M., Lin,P., Ren,Y., He,Y., Wang,S., Xu,Y., Cao,B.,
            Wang,G., Moran,M.F. and Mao,X.
  TITLE     The ubiquitin ligase HERC4 suppresses MafA transcriptional activity
            triggered by GSK3beta in myeloma by atypical K63-linked
            polyubiquitination
  JOURNAL   J Biol Chem 299 (5), 104675 (2023)
   PUBMED   37028761
  REMARK    GeneRIF: The ubiquitin ligase HERC4 suppresses MafA transcriptional
            activity triggered by GSK3beta in myeloma by atypical K63-linked
            polyubiquitination.
REFERENCE   3  (bases 1 to 4424)
  AUTHORS   Xu,Y., Ji,K., Wu,M., Hao,B., Yao,K.T. and Xu,Y.
  TITLE     A miRNA-HERC4 pathway promotes breast tumorigenesis by inactivating
            tumor suppressor LATS1
  JOURNAL   Protein Cell 10 (8), 595-605 (2019)
   PUBMED   30710319
  REMARK    GeneRIF: The authors findings demonstrate that HERC4 is important
            for promoting tumorigenesis.
REFERENCE   4  (bases 1 to 4424)
  AUTHORS   Sun,X., Sun,B., Cui,M. and Zhou,Z.
  TITLE     HERC4 exerts an anti-tumor role through destabilizing the
            oncoprotein Smo
  JOURNAL   Biochem Biophys Res Commun 513 (4), 1013-1018 (2019)
   PUBMED   31010679
  REMARK    GeneRIF: HERC4 is downregulated in NSCLC samples; HERC4 interacts
            with Smo reciprocally in NSCLC cells; knockdown of herc4 activates
            Hh pathway and promotes NSCLC cell proliferation. Taken together,
            our studies have demonstrated that HERC4 acts as a tumor suppressor
            via destabilizing the oncoprotein Smo
REFERENCE   5  (bases 1 to 4424)
  AUTHORS   Yang,G., Hamadeh,I.S., Katz,J., Riva,A., Lakatos,P., Balla,B.,
            Kosa,J., Vaszilko,M., Pelliccioni,G.A., Davis,N., Langaee,T.Y.,
            Moreb,J.S. and Gong,Y.
  TITLE     SIRT1/HERC4 Locus Associated With Bisphosphonate-Induced
            Osteonecrosis of the Jaw: An Exome-Wide Association Analysis
  JOURNAL   J Bone Miner Res 33 (1), 91-98 (2018)
   PUBMED   28856724
  REMARK    GeneRIF: the SIRT1/HERC4 locus on chromosome 10 was identified to
            be associated with intravenous (iv) bisphosphonates induced
            Osteonecrosis of the Jaw.
REFERENCE   6  (bases 1 to 4424)
  AUTHORS   Zeng,W.L., Chen,Y.W., Zhou,H., Zhou,J.Y., Wei,M. and Shi,R.
  TITLE     Expression of HERC4 in lung cancer and its correlation with
            clinicopathological parameters
  JOURNAL   Asian Pac J Cancer Prev 16 (2), 513-517 (2015)
   PUBMED   25684480
  REMARK    GeneRIF: High HERC4 expression is associated with lung cancer.
REFERENCE   7  (bases 1 to 4424)
  AUTHORS   Rodriguez,C.I. and Stewart,C.L.
  TITLE     Disruption of the ubiquitin ligase HERC4 causes defects in
            spermatozoon maturation and impaired fertility
  JOURNAL   Dev Biol 312 (2), 501-508 (2007)
   PUBMED   17967448
REFERENCE   8  (bases 1 to 4424)
  AUTHORS   Grupe,A., Li,Y., Rowland,C., Nowotny,P., Hinrichs,A.L., Smemo,S.,
            Kauwe,J.S., Maxwell,T.J., Cherny,S., Doil,L., Tacey,K., van
            Luchene,R., Myers,A., Wavrant-De Vrieze,F., Kaleem,M.,
            Hollingworth,P., Jehu,L., Foy,C., Archer,N., Hamilton,G.,
            Holmans,P., Morris,C.M., Catanese,J., Sninsky,J., White,T.J.,
            Powell,J., Hardy,J., O'Donovan,M., Lovestone,S., Jones,L.,
            Morris,J.C., Thal,L., Owen,M., Williams,J. and Goate,A.
  TITLE     A scan of chromosome 10 identifies a novel locus showing strong
            association with late-onset Alzheimer disease
  JOURNAL   Am J Hum Genet 78 (1), 78-88 (2006)
   PUBMED   16385451
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   9  (bases 1 to 4424)
  AUTHORS   Hochrainer,K., Mayer,H., Baranyi,U., Binder,B., Lipp,J. and
            Kroismayr,R.
  TITLE     The human HERC family of ubiquitin ligases: novel members, genomic
            organization, expression profiling, and evolutionary aspects
  JOURNAL   Genomics 85 (2), 153-164 (2005)
   PUBMED   15676274
  REMARK    GeneRIF: HERC4 ancestor emerged in nematodes and that the family
            expanded throughout evolution. The mRNA expression pattern of the
            HERC4 was found to be diverse in selected tissues and cells
REFERENCE   10 (bases 1 to 4424)
  AUTHORS   Deloukas,P., Earthrowl,M.E., Grafham,D.V., Rubenfield,M.,
            French,L., Steward,C.A., Sims,S.K., Jones,M.C., Searle,S.,
            Scott,C., Howe,K., Hunt,S.E., Andrews,T.D., Gilbert,J.G.,
            Swarbreck,D., Ashurst,J.L., Taylor,A., Battles,J., Bird,C.P.,
            Ainscough,R., Almeida,J.P., Ashwell,R.I., Ambrose,K.D.,
            Babbage,A.K., Bagguley,C.L., Bailey,J., Banerjee,R., Bates,K.,
            Beasley,H., Bray-Allen,S., Brown,A.J., Brown,J.Y., Burford,D.C.,
            Burrill,W., Burton,J., Cahill,P., Camire,D., Carter,N.P.,
            Chapman,J.C., Clark,S.Y., Clarke,G., Clee,C.M., Clegg,S., Corby,N.,
            Coulson,A., Dhami,P., Dutta,I., Dunn,M., Faulkner,L., Frankish,A.,
            Frankland,J.A., Garner,P., Garnett,J., Gribble,S., Griffiths,C.,
            Grocock,R., Gustafson,E., Hammond,S., Harley,J.L., Hart,E.,
            Heath,P.D., Ho,T.P., Hopkins,B., Horne,J., Howden,P.J., Huckle,E.,
            Hynds,C., Johnson,C., Johnson,D., Kana,A., Kay,M., Kimberley,A.M.,
            Kershaw,J.K., Kokkinaki,M., Laird,G.K., Lawlor,S., Lee,H.M.,
            Leongamornlert,D.A., Laird,G., Lloyd,C., Lloyd,D.M., Loveland,J.,
            Lovell,J., McLaren,S., McLay,K.E., McMurray,A.,
            Mashreghi-Mohammadi,M., Matthews,L., Milne,S., Nickerson,T.,
            Nguyen,M., Overton-Larty,E., Palmer,S.A., Pearce,A.V., Peck,A.I.,
            Pelan,S., Phillimore,B., Porter,K., Rice,C.M., Rogosin,A.,
            Ross,M.T., Sarafidou,T., Sehra,H.K., Shownkeen,R., Skuce,C.D.,
            Smith,M., Standring,L., Sycamore,N., Tester,J., Thorpe,A.,
            Torcasso,W., Tracey,A., Tromans,A., Tsolas,J., Wall,M., Walsh,J.,
            Wang,H., Weinstock,K., West,A.P., Willey,D.L., Whitehead,S.L.,
            Wilming,L., Wray,P.W., Young,L., Chen,Y., Lovering,R.C.,
            Moschonas,N.K., Siebert,R., Fechtel,K., Bentley,D., Durbin,R.,
            Hubbard,T., Doucette-Stamm,L., Beck,S., Smith,D.R. and Rogers,J.
  TITLE     The DNA sequence and comparative analysis of human chromosome 10
  JOURNAL   Nature 429 (6990), 375-381 (2004)
   PUBMED   15164054
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DB459623.1, BC039600.1 and BC018114.1.
            
            On Jun 2, 2019 this sequence version replaced NM_015601.3.
            
            Summary: HERC4 belongs to the HERC family of ubiquitin ligases, all
            of which contain a HECT domain and at least 1 RCC1 (MIM
            179710)-like domain (RLD). The 350-amino acid HECT domain is
            predicted to catalyze the formation of a thioester with ubiquitin
            before transferring it to a substrate, and the RLD is predicted to
            act as a guanine nucleotide exchange factor for small G proteins
            (Hochrainer et al., 2005 [PubMed 15676274]).[supplied by OMIM, Mar
            2008].
            
            Transcript Variant: This variant (2) lacks an alternate in-frame
            exon compared to variant 1. The resulting isoform (b) has the same
            N- and C-termini but is shorter compared to isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC039600.1, AK292809.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000373700.9/ ENSP00000362804.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-27                DB459623.1         64-90
            28-3840             BC039600.1         1-3813
            3841-4424           BC018114.1         1860-2443
FEATURES             Location/Qualifiers
     source          1..4424
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q21.3"
     gene            1..4424
                     /gene="HERC4"
                     /note="HECT and RLD domain containing E3 ubiquitin protein
                     ligase 4"
                     /db_xref="GeneID:26091"
                     /db_xref="HGNC:HGNC:24521"
                     /db_xref="MIM:609248"
     exon            1..140
                     /gene="HERC4"
                     /inference="alignment:Splign:2.1.0"
     exon            141..170
                     /gene="HERC4"
                     /inference="alignment:Splign:2.1.0"
     exon            171..474
                     /gene="HERC4"
                     /inference="alignment:Splign:2.1.0"
     CDS             249..3398
                     /gene="HERC4"
                     /EC_number="2.3.2.26"
                     /note="isoform b is encoded by transcript variant 2;
                     probable E3 ubiquitin-protein ligase HERC4; HECT domain
                     and RCC1-like domain-containing protein 4; hect domain and
                     RLD 4; HECT-type E3 ubiquitin transferase HERC4"
                     /codon_start=1
                     /product="probable E3 ubiquitin-protein ligase HERC4
                     isoform b"
                     /protein_id="NP_056416.2"
                     /db_xref="CCDS:CCDS7274.1"
                     /db_xref="GeneID:26091"
                     /db_xref="HGNC:HGNC:24521"
                     /db_xref="MIM:609248"
                     /translation="MLCWGNASFGQLGLGGIDEEIVLEPRKSDFFINKRVRDVGCGLR
                     HTVFVLDDGTVYTCGCNDLGQLGHEKSRKKPEQVVALDAQNIVAVSCGEAHTLALNDK
                     GQVYAWGLDSDGQLGLVGSEECIRVPRNIKSLSDIQIVQVACGYYHSLALSKASEVFC
                     WGQNKYGQLGLGTDCKKQTSPQLLKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNK
                     FGQLGLNDENDRYVPNLLKSLRSQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGH
                     NSTSHEINPRKVFELMGSIVTEIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSN
                     RKSPFTVKGNWYPYNGQCLPDIDSEEYFCVKRIFSGGDQSFSHYSSPQNCGPPDDFRC
                     PNPTKQIWTVNEALIQKWLSYPSGRFPVEIANEIDGTFSSSGCLNGSFLAVSNDDHYR
                     TGTRFSGVDMNAARLLFHKLIQPDHPQISQQVAASLEKNLIPKLTSSLPDVEALRFYL
                     TLPECPLMSDSNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIVELFKEV
                     VVHLLKLYKIGIPPSERRIFNSFLHTALKVLEILHRVNEKMGQIIQYDKFYIHEVQEL
                     IDIRNDYINWVQQQAYGMLADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAH
                     RQNVSSLFLPVIESVNPCLILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAV
                     DAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDSDLFHLIGVICGLAI
                     YNCTIVDLHFPLALYKKLLKKKPSLDDLKELMPDVGRSMQQLLDYPEDDIEETFCLNF
                     TITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHK
                     VCGGKVLLLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELP
                     LEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKE
                     TLRSKLIQAIDHNEGFSLI"
     exon            475..634
                     /gene="HERC4"
                     /inference="alignment:Splign:2.1.0"
     exon            635..711
                     /gene="HERC4"
                     /inference="alignment:Splign:2.1.0"
     exon            712..933
                     /gene="HERC4"
                     /inference="alignment:Splign:2.1.0"
     exon            934..1025
                     /gene="HERC4"
                     /inference="alignment:Splign:2.1.0"
     exon            1026..1156
                     /gene="HERC4"
                     /inference="alignment:Splign:2.1.0"
     exon            1157..1317
                     /gene="HERC4"
                     /inference="alignment:Splign:2.1.0"
     exon            1318..1394
                     /gene="HERC4"
                     /inference="alignment:Splign:2.1.0"
     exon            1395..1519
                     /gene="HERC4"
                     /inference="alignment:Splign:2.1.0"
     exon            1520..1579
                     /gene="HERC4"
                     /inference="alignment:Splign:2.1.0"
     exon            1580..1691
                     /gene="HERC4"
                     /inference="alignment:Splign:2.1.0"
     exon            1692..1881
                     /gene="HERC4"
                     /inference="alignment:Splign:2.1.0"
     exon            1882..2054
                     /gene="HERC4"
                     /inference="alignment:Splign:2.1.0"
     exon            2055..2174
                     /gene="HERC4"
                     /inference="alignment:Splign:2.1.0"
     exon            2175..2273
                     /gene="HERC4"
                     /inference="alignment:Splign:2.1.0"
     exon            2274..2441
                     /gene="HERC4"
                     /inference="alignment:Splign:2.1.0"
     exon            2442..2585
                     /gene="HERC4"
                     /inference="alignment:Splign:2.1.0"
     exon            2586..2752
                     /gene="HERC4"
                     /inference="alignment:Splign:2.1.0"
     exon            2753..2819
                     /gene="HERC4"
                     /inference="alignment:Splign:2.1.0"
     exon            2820..2902
                     /gene="HERC4"
                     /inference="alignment:Splign:2.1.0"
     exon            2903..3086
                     /gene="HERC4"
                     /inference="alignment:Splign:2.1.0"
     exon            3087..3189
                     /gene="HERC4"
                     /inference="alignment:Splign:2.1.0"
     exon            3190..4424
                     /gene="HERC4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3820..3825
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HERC4"
                     /note="hexamer: AATAAA"
     polyA_site      3845
                     /gene="HERC4"
                     /note="major polyA site"
     regulatory      4396..4401
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HERC4"
                     /note="hexamer: AATAAA"
     polyA_site      4424
                     /gene="HERC4"
ORIGIN      
        1 agtctcttcc ccaactctcc ccacccctgg tgctctccgc cccgcttgcc tcccgttctt
       61 ccccgcccgc tccatccggg tcgctgacgg ctgtctctgg actggacagc agtggcgtcc
      121 gcttttctct aaggaacccg ataagaactc cattttctgt tgatgtggag acatagggta
      181 cctccaactt tccggggttt cagaagagaa ttttttattg aaactggaag accaaaataa
      241 ttacaagcat gttgtgctgg ggaaatgcat cctttgggca gctaggtttg ggtggaattg
      301 atgaagaaat tgtactagag cccagaaaaa gtgacttctt tataaataaa agggtccgag
      361 atgtaggatg tggactcaga catactgtgt ttgttctgga tgatggaaca gtgtacacat
      421 gtggatgtaa tgatctagga cagctaggtc atgaaaaatc cagaaagaaa ccagagcagg
      481 ttgttgccct ggatgcccaa aatattgtag ctgtttcatg tggagaagct catacgttag
      541 cgctaaatga caaaggccag gtgtatgctt ggggtctcga ttctgatgga cagcttggcc
      601 tggtaggatc agaggaatgc atcagagtac ccagaaatat taaaagtttg tcagatatcc
      661 agattgtaca ggttgcttgt ggttactatc attcacttgc actttctaaa gcaagtgaag
      721 tcttctgttg gggacagaat aaatatggcc aattgggttt aggtactgac tgtaaaaagc
      781 aaacttcacc gcagctgctt aagtctttgc ttggaatccc tttcatgcaa gttgcagcag
      841 gaggagccca tagttttgta ctcacccttt ctggagctat ctttggatgg ggacgcaaca
      901 agtttggtca gctaggtctt aatgatgaaa atgataggta tgttcctaat ttactaaagt
      961 cactaagatc tcagaaaata gtttatattt gttgtggaga agatcatact gctgctctaa
     1021 ccaaggaagg tggagtgttt acttttggag ctggagggta tggtcagttg ggccataatt
     1081 ctaccagtca tgaaataaac ccaaggaaag tttttgaact tatgggaagc attgtcactg
     1141 agattgcttg tggacggcag cacacttctg cttttgttcc ttcatcagga cgaatttact
     1201 cttttgggct tggtggtaat gggcagctgg gaaccggttc aacaagcaac aggaaaagcc
     1261 cctttactgt aaaaggaaat tggtacccct ataatgggca gtgtctacca gatattgatt
     1321 ctgaagaata tttctgtgta aaaagaattt tctcaggggg agatcaaagc ttttcacatt
     1381 actctagtcc ccagaactgt gggccaccag atgacttcag atgtcccaat ccgacaaagc
     1441 agatctggac agtgaatgaa gctctaattc agaaatggct gagctatcct tctggaaggt
     1501 ttcctgtgga gatagccaat gagatagatg gaacgttttc ttcctctggt tgcctaaatg
     1561 gaagtttttt agctgttagc aatgatgatc actatagaac aggtaccaga ttttcagggg
     1621 ttgatatgaa tgctgctagg cttttattcc acaaacttat acaacctgat catccgcaga
     1681 tatctcagca ggtggcagct agtttggaaa agaatcttat tcctaaactg actagctcct
     1741 tacctgatgt tgaagcattg aggttttatc ttactctacc agaatgtccc ctgatgagtg
     1801 attccaacaa tttcacaaca atagcaattc cctttggtac agctcttgtg aacctagaaa
     1861 aggcaccact gaaagtactt gaaaactggt ggtcagtact tgaacctcca ctattcctca
     1921 agatagtaga actttttaag gaagttgtgg tacatctttt gaaactctac aagatcggta
     1981 ttcccccttc tgaaagaaga attttcaaca gttttcttca tactgcatta aaggttttag
     2041 aaatactaca tagggtaaat gagaaaatgg gacagattat acagtatgat aaattttata
     2101 tacatgaagt acaagaattg atagacataa gaaatgatta tatcaactgg gtccaacagc
     2161 aggcctatgg aatgttggca gatatccctg ttacaatctg tacatatcca tttgtatttg
     2221 atgcccaagc aaaaactact ctgttacaga ccgatgcagt cttacagatg cagatggcta
     2281 ttgatcaggc ccacaggcag aatgtctcct ctctttttct cccagtgatt gaatctgtga
     2341 atccctgctt aattctagtg gtgcgtagag aaaatattgt aggagatgca atggaagtcc
     2401 ttaggaaaac aaagaacata gattacaaga agccactcaa ggttatattt gttggagaag
     2461 atgctgtgga tgcaggaggg gtgcgcaaag aatttttctt gctcatcatg agggaattat
     2521 tggatcctaa atacggcatg tttaggtatt atgaagattc caggctcatt tggttttctg
     2581 ataagacatt tgaagacagt gatttgttcc atttgattgg tgttatctgt ggcttagcaa
     2641 tttataattg taccattgtg gacctccatt ttcctttggc tttatataag aaactactga
     2701 aaaagaagcc atccttggat gatttgaaag aactaatgcc tgatgttggg agaagcatgc
     2761 aacagttact ggattatcca gaagatgaca tagaggaaac attttgtctt aattttacga
     2821 tcacagttga aaactttggt gcaacagaag tgaaagagct ggttctaaat ggtgcagaca
     2881 cagctgttaa caaacaaaat cggcaagagt ttgtcgatgc ttatgtggat tacatattca
     2941 ataaatcagt ggcttcctta tttgatgctt ttcatgcggg ctttcataag gtctgtggag
     3001 gaaaagtcct tctgctcttt cagcctaatg aactacaagc aatggtcatt ggaaatacaa
     3061 attatgattg gaaggaactg gaaaagaata cagaatacaa aggggaatat tgggcagaac
     3121 atcctacgat aaaaattttt tgggaagtat ttcacgaatt accattggaa aagaagaaac
     3181 agtttctgtt atttttgaca ggtagtgatc gcattcctat tcttggtatg aagagtctga
     3241 aactagtcat ccagtccaca ggaggtggtg aggagtatct cccagtttcc catacttgtt
     3301 ttaatcttct ggatcttcca aaatatacag aaaaagaaac tctacgctct aaactgatcc
     3361 aagctattga tcacaatgaa ggcttcagtt taatataact ttggagttat aactattcag
     3421 tttagtgcaa aagcattaaa ctatttgtgt ttttcttgtg gtgatgaatt cagcaaggtg
     3481 acagaggtac tattataatt cttacttgca gaatgttcaa tctacgagtg ttcatggaag
     3541 ccaaaaaata ttaaaggaaa atgaacaaac tgttaatatt attgtacaga accatggatt
     3601 ttttttgacc atcttctaat aaacatagca agtattatga atacattaaa gttttactaa
     3661 catgaatttt aagagtttgc atatttcaga aatgatctgg tgtgagtgca tggaaatatt
     3721 gcttaatttt tcttcaatca ttgagtgaaa aacctttaac tttggcctgc aatagtcatt
     3781 tgattatttt ttcattttgt aaataatgtt aagttttgta ataaaatagt tatgttctga
     3841 taccagtaca gtttctatgg ttgtaattga acttgaacag acttttaaag gttaaaaatt
     3901 atgatttaaa tcttactctg agactctata aaaagaaaaa aaaggtagca tggtggaaac
     3961 actatctttt cttttttgct agaataagtg tctttgtgca aacctaaatc acagataggg
     4021 aatatactac ataacctgca gttcttttct ttgtgaatcc tgacacactg atagatgggg
     4081 gattgtcgat cagagaactt attaatattt agtactggaa gaactctgtc tccacagttg
     4141 ccagtaataa aaagaaacat tggctactat gagcaccaat cactgggtta tagctttcaa
     4201 aattattgat gctgcagtgc tttagagcta tttccttgaa tttaagaaac aaatcttaac
     4261 agttttatgg tgctgatgct tagttgtctc atgccattaa attgtaaaag tgagttgatg
     4321 caatacatgt gactttctgc tataatgcaa atatttattt tttaaattta ttttaaaatg
     4381 ccgtgaaaac tgtttaataa agatttattg ttttaatatt taaa
//
