LOCUS       NM_001367233            4431 bp    mRNA    linear   PRI 22-SEP-2024
DEFINITION  Homo sapiens hephaestin (HEPH), transcript variant 6, mRNA.
ACCESSION   NM_001367233 XM_006724722
VERSION     NM_001367233.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4431)
  AUTHORS   Baringer,S.L., Palsa,K., Spiegelman,V.S., Simpson,I.A. and
            Connor,J.R.
  TITLE     Apo- and holo-transferrin differentially interact with hephaestin
            and ferroportin in a novel mechanism of cellular iron release
            regulation
  JOURNAL   J Biomed Sci 30 (1), 36 (2023)
   PUBMED   37277838
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4431)
  AUTHORS   Aslan,E.S., Aydin,H., Tekin,Y.K., Keles,S., White,K.N. and Hekim,N.
  TITLE     Association between iron metabolism and SARS-COV-2 infection,
            determined by ferritin, hephaestin and hypoxia-induced factor-1
            alpha levels in COVID-19 patients
  JOURNAL   Mol Biol Rep 50 (3), 2471-2478 (2023)
   PUBMED   36600108
  REMARK    GeneRIF: Association between iron metabolism and SARS-COV-2
            infection, determined by ferritin, hephaestin and hypoxia-induced
            factor-1 alpha levels in COVID-19 patients.
REFERENCE   3  (bases 1 to 4431)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 4431)
  AUTHORS   Kondaiah,P., Sharp,P.A. and Pullakhandam,R.
  TITLE     Zinc induces iron egress from intestinal Caco-2 cells via induction
            of Hephaestin: A role for PI3K in intestinal iron absorption
  JOURNAL   Biochem Biophys Res Commun 523 (4), 987-992 (2020)
   PUBMED   31973819
  REMARK    GeneRIF: Zinc induces iron egress from intestinal Caco-2 cells via
            induction of Hephaestin: A role for PI3K in intestinal iron
            absorption.
REFERENCE   5  (bases 1 to 4431)
  AUTHORS   Dlouhy,A.C., Bailey,D.K., Steimle,B.L., Parker,H.V. and Kosman,D.J.
  TITLE     Fluorescence resonance energy transfer links membrane ferroportin,
            hephaestin but not ferroportin, amyloid precursor protein complex
            with iron efflux
  JOURNAL   J Biol Chem 294 (11), 4202-4214 (2019)
   PUBMED   30647129
  REMARK    GeneRIF: Iron supplementation increased surface expression of the
            iron-efflux complex, and copper depletion knocked down hephaestin
            (Heph) activity and decreased ferroportin (Fpn) membrane
            localization.
REFERENCE   6  (bases 1 to 4431)
  AUTHORS   Zoller,H., Theurl,I., Koch,R.O., McKie,A.T., Vogel,W. and Weiss,G.
  TITLE     Duodenal cytochrome b and hephaestin expression in patients with
            iron deficiency and hemochromatosis
  JOURNAL   Gastroenterology 125 (3), 746-754 (2003)
   PUBMED   12949720
REFERENCE   7  (bases 1 to 4431)
  AUTHORS   Syed,B.A., Beaumont,N.J., Patel,A., Naylor,C.E., Bayele,H.K.,
            Joannou,C.L., Rowe,P.S., Evans,R.W. and Srai,S.K.
  TITLE     Analysis of the human hephaestin gene and protein: comparative
            modelling of the N-terminus ecto-domain based upon ceruloplasmin
  JOURNAL   Protein Eng 15 (3), 205-214 (2002)
   PUBMED   11932491
  REMARK    GeneRIF: The gene structure, spanning approximately 100 kb, was
            assembled from the cDNA clones and the chromosome X genomic
            sequence data. Modelling supports its role as a membrane-tethered
            ferroxidase.
REFERENCE   8  (bases 1 to 4431)
  AUTHORS   Simovich,M.J., Conrad,M.E., Umbreit,J.N., Moore,E.G.,
            Hainsworth,L.N. and Smith,H.K.
  TITLE     Cellular location of proteins related to iron absorption and
            transport
  JOURNAL   Am J Hematol 69 (3), 164-170 (2002)
   PUBMED   11891802
  REMARK    GeneRIF: location was observed on or near the cell surface
            suggesting it might participate in surface membrane transport of
            iron
REFERENCE   9  (bases 1 to 4431)
  AUTHORS   Schueler,M.G., Higgins,A.W., Nagaraja,R., Tentler,D., Dahl,N.,
            Gustashaw,K. and Willard,H.F.
  TITLE     Large-insert clone/STS contigs in Xq11-q12, spanning deletions in
            patients with androgen insensitivity and mental retardation
  JOURNAL   Genomics 66 (1), 104-109 (2000)
   PUBMED   10843811
REFERENCE   10 (bases 1 to 4431)
  AUTHORS   Vulpe,C.D., Kuo,Y.M., Murphy,T.L., Cowley,L., Askwith,C.,
            Libina,N., Gitschier,J. and Anderson,G.J.
  TITLE     Hephaestin, a ceruloplasmin homologue implicated in intestinal iron
            transport, is defective in the sla mouse
  JOURNAL   Nat Genet 21 (2), 195-199 (1999)
   PUBMED   9988272
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL030998.1, KF459144.1 and
            AL157698.8.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Apr 13, 2021 this sequence version replaced NM_001367233.2.
            
            Summary: This gene encodes a member of the multicopper oxidase
            protein family. The encoded protein is involved in the transport of
            dietary iron from epithelial cells of the intestinal lumen into the
            circulatory system, and may be involved in copper transport and
            homeostasis. In mouse, defects in this gene can lead to severe
            microcytic anemia. Alternative splicing results in multiple
            transcript variants. [provided by RefSeq, Aug 2013].
            
            Transcript Variant: This variant (6), as well as variants 5 and 7,
            encodes isoform e.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR18074969.2401397.1,
                                           SRR1803616.155480.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            MANE Ensembl match               :: ENST00000343002.7/
                                                ENSP00000343939.2
            RefSeq Select criteria           :: based on manual assertion,
                                                conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-224               AL030998.1         87387-87610         c
            225-404             AL030998.1         81120-81299         c
            405-649             AL030998.1         79258-79502         c
            650-741             AL030998.1         78177-78268         c
            742-862             KF459144.1         1-121
            863-1045            AL030998.1         63316-63498         c
            1046-1300           AL030998.1         61919-62173         c
            1301-1469           AL030998.1         59559-59727         c
            1470-1606           AL030998.1         58219-58355         c
            1607-1738           AL030998.1         56628-56759         c
            1739-1950           AL030998.1         53963-54174         c
            1951-2101           AL030998.1         52829-52979         c
            2102-2314           AL030998.1         51105-51317         c
            2315-2528           AL030998.1         48280-48493         c
            2529-2668           AL030998.1         44523-44662         c
            2669-2800           AL030998.1         43611-43742         c
            2801-2907           AL157698.8         3279-3385
            2908-3133           AL157698.8         4349-4574
            3134-3273           AL157698.8         7084-7223
            3274-3436           AL157698.8         8344-8506
            3437-3481           AL157698.8         11888-11932
            3482-4431           AL157698.8         14684-15633
FEATURES             Location/Qualifiers
     source          1..4431
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq12"
     gene            1..4431
                     /gene="HEPH"
                     /gene_synonym="CPL; Hp"
                     /note="hephaestin"
                     /db_xref="GeneID:9843"
                     /db_xref="HGNC:HGNC:4866"
                     /db_xref="MIM:300167"
     exon            1..224
                     /gene="HEPH"
                     /gene_synonym="CPL; Hp"
                     /inference="alignment:Splign:2.1.0"
     exon            225..404
                     /gene="HEPH"
                     /gene_synonym="CPL; Hp"
                     /inference="alignment:Splign:2.1.0"
     CDS             238..3714
                     /gene="HEPH"
                     /gene_synonym="CPL; Hp"
                     /EC_number="1.16.3.1"
                     /note="isoform a precursor is encoded by transcript
                     variant 6"
                     /codon_start=1
                     /product="hephaestin isoform a precursor"
                     /protein_id="NP_001354162.2"
                     /db_xref="CCDS:CCDS14384.4"
                     /db_xref="GeneID:9843"
                     /db_xref="HGNC:HGNC:4866"
                     /db_xref="MIM:300167"
                     /translation="MESGHLLWALLFMQSLWPQLTDGATRVYYLGIRDVQWNYAPKGR
                     NVITNQPLDSDIVASSFLKSDKNRIGGTYKKTIYKEYKDDSYTDEVAQPAWLGFLGPV
                     LQAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSLYPDGSSGPLKADDSVPPGGS
                     HIYNWTIPEGHAPTDADPACLTWIYHSHVDAPRDIATGLIGPLITCKRGALDGNSPPQ
                     RQDVDHDFFLLFSVVDENLSWHLNENIATYCSDPASVDKEDETFQESNRMHAINGFVF
                     GNLPELNMCAQKRVAWHLFGMGNEIDVHTAFFHGQMLTTRGHHTDVANIFPATFVTAE
                     MVPWEPGTWLISCQVNSHFRDGMQALYKVKSCSMAPPVDLLTGKVRQYFIEAHEIQWD
                     YGPMGHDGSTGKNLREPGSISDKFFQKSSSRIGGTYWKVRYEAFQDETFQEKMHLEED
                     RHLGILGPVIRAEVGDTIQVVFYNRASQPFSMQPHGVFYEKDYEGTVYNDGSSYPGLV
                     AKPFEKVTYRWTVPPHAGPTAQDPACLTWMYFSAADPIRDTNSGLVGPLLVCRAGALG
                     ADGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDFRLLSEDIEGFQDSNRMHAIN
                     GFLFSNLPRLDMCKGDTVAWHLLGLGTETDVHGVMFQGNTVQLQGMRKGAAMLFPHTF
                     VMAIMQPDNLGTFEIYCQAGSHREAGMRAIYNVSQCPGHQATPRQRYQAARIYYIMAE
                     EVEWDYCPDRSWEREWHNQSEKDSYGYIFLSNKDGLLGSRYKKAVFREYTDGTFRIPR
                     PRTGPEEHLGILGPLIKGEVGDILTVVFKNNASRPYSVHAHGVLESTTVWPLAAEPGE
                     VVTYQWNIPERSGPGPNDSACVSWIYYSAVDPIKDMYSGLVGPLAICQKGILEPHGGR
                     SDMDREFALLFLIFDENKSWYLEENVATHGSQDPGSINLQDETFLESNKMHAINGKLY
                     ANLRGLTMYQGERVAWYMLAMGQDVDLHTIHFHAESFLYRNGENYRADVVDLFPGTFE
                     VVEMVASNPGTWLMHCHVTDHVHAGMETLFTVFSRTEHLSPLTVITKETEKAVPPRDI
                     EEGNVKMLGMQIPIKNVEMLASVLVAISVTLLLVVLALGGVVWYQHRQRKLRRNRRSI
                     LDDSFKLLSFKQ"
     exon            405..649
                     /gene="HEPH"
                     /gene_synonym="CPL; Hp"
                     /inference="alignment:Splign:2.1.0"
     exon            650..862
                     /gene="HEPH"
                     /gene_synonym="CPL; Hp"
                     /inference="alignment:Splign:2.1.0"
     exon            863..1045
                     /gene="HEPH"
                     /gene_synonym="CPL; Hp"
                     /inference="alignment:Splign:2.1.0"
     exon            1046..1300
                     /gene="HEPH"
                     /gene_synonym="CPL; Hp"
                     /inference="alignment:Splign:2.1.0"
     exon            1301..1469
                     /gene="HEPH"
                     /gene_synonym="CPL; Hp"
                     /inference="alignment:Splign:2.1.0"
     exon            1470..1606
                     /gene="HEPH"
                     /gene_synonym="CPL; Hp"
                     /inference="alignment:Splign:2.1.0"
     exon            1607..1738
                     /gene="HEPH"
                     /gene_synonym="CPL; Hp"
                     /inference="alignment:Splign:2.1.0"
     exon            1739..1950
                     /gene="HEPH"
                     /gene_synonym="CPL; Hp"
                     /inference="alignment:Splign:2.1.0"
     exon            1951..2101
                     /gene="HEPH"
                     /gene_synonym="CPL; Hp"
                     /inference="alignment:Splign:2.1.0"
     exon            2102..2314
                     /gene="HEPH"
                     /gene_synonym="CPL; Hp"
                     /inference="alignment:Splign:2.1.0"
     exon            2315..2528
                     /gene="HEPH"
                     /gene_synonym="CPL; Hp"
                     /inference="alignment:Splign:2.1.0"
     exon            2529..2668
                     /gene="HEPH"
                     /gene_synonym="CPL; Hp"
                     /inference="alignment:Splign:2.1.0"
     exon            2669..2800
                     /gene="HEPH"
                     /gene_synonym="CPL; Hp"
                     /inference="alignment:Splign:2.1.0"
     exon            2801..2907
                     /gene="HEPH"
                     /gene_synonym="CPL; Hp"
                     /inference="alignment:Splign:2.1.0"
     exon            2908..3133
                     /gene="HEPH"
                     /gene_synonym="CPL; Hp"
                     /inference="alignment:Splign:2.1.0"
     exon            3134..3273
                     /gene="HEPH"
                     /gene_synonym="CPL; Hp"
                     /inference="alignment:Splign:2.1.0"
     exon            3274..3436
                     /gene="HEPH"
                     /gene_synonym="CPL; Hp"
                     /inference="alignment:Splign:2.1.0"
     exon            3437..3481
                     /gene="HEPH"
                     /gene_synonym="CPL; Hp"
                     /inference="alignment:Splign:2.1.0"
     exon            3482..4431
                     /gene="HEPH"
                     /gene_synonym="CPL; Hp"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4405..4410
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HEPH"
                     /gene_synonym="CPL; Hp"
                     /note="hexamer: ATTAAA"
     polyA_site      4431
                     /gene="HEPH"
                     /gene_synonym="CPL; Hp"
                     /note="major polyA site"
ORIGIN      
        1 attccagtag ttttgtttct ggaaaagagg gcacccagcc cttccccctc cctcatcctc
       61 ccatcccagt aaaccctgcc aaattggaat cctggactta atttaggaga aaggccctgt
      121 aaccaagata ctgactgaac atggctggcg gactcaggct ggggtctgca gtgcagcatt
      181 aatgggccgc tgacatgaat atggagtagt tttctctagc aaagagtaat gtgggccatg
      241 gagtcaggcc acctcctctg ggctctgctg ttcatgcagt ccttgtggcc tcaactgact
      301 gatggagcca ctcgagtcta ctacctgggc atccgggatg tgcagtggaa ctatgctccc
      361 aagggaagaa atgtcatcac gaaccagcct ctggacagtg acatagtggc ttccagcttc
      421 ttaaagtctg acaagaaccg gataggggga acctacaaga agaccatcta taaagaatac
      481 aaggatgact catacacaga tgaagtggcc cagcctgcct ggttgggctt cctggggcca
      541 gtgttgcagg ctgaagtggg ggatgtcatt cttattcacc tgaagaattt tgccactcgt
      601 ccctatacca tccaccctca tggtgtcttc tacgagaagg actctgaagg ttccctatac
      661 ccagatggct cctctgggcc actgaaagct gatgactctg ttcccccggg gggcagccat
      721 atctacaact ggaccattcc agaaggccat gcacccaccg atgctgaccc agcgtgcctc
      781 acctggatct accattctca tgtagatgct ccacgagaca ttgcaactgg cctaattggg
      841 cctctcatca cctgtaaaag aggagccctg gatgggaact cccctcctca acgccaggat
      901 gtagaccatg atttcttcct cctcttcagt gtggtagatg agaacctcag ctggcatctc
      961 aatgagaaca ttgccactta ctgctcagat cctgcttcag tggacaaaga agatgagaca
     1021 tttcaggaga gcaataggat gcatgcaatc aatggctttg tttttgggaa tttacctgag
     1081 ctgaacatgt gtgcacagaa acgtgtggcc tggcacttgt ttggcatggg caatgaaatt
     1141 gatgtccaca cagcattttt ccatggacag atgctgacta cccgtggaca ccacactgat
     1201 gtggctaaca tctttccagc cacctttgtg actgctgaga tggtgccctg ggaacctggt
     1261 acctggttaa ttagctgcca agtgaacagt cactttcgag atggcatgca ggcactctac
     1321 aaggtcaagt cttgctccat ggcccctcct gtggacctgc tcacaggcaa agttcgacag
     1381 tacttcattg aggcccatga gattcaatgg gactatggcc cgatggggca tgatgggagt
     1441 actgggaaga atttgagaga gccaggcagt atctcagata agtttttcca gaagagctcc
     1501 agccgaattg ggggcactta ctggaaagtg cgatatgaag cctttcaaga tgagacattc
     1561 caagagaaga tgcatttgga ggaagatagg catcttggaa tcctggggcc agtgatccgg
     1621 gctgaggtgg gtgacaccat tcaggtggtc ttctacaacc gtgcctccca gccattcagc
     1681 atgcagcccc atggggtctt ttatgagaaa gactatgaag gcactgtgta caatgatggc
     1741 tcatcttacc ctggcttggt tgccaagccc tttgagaaag taacataccg ctggacagtc
     1801 ccccctcatg ccggtcccac tgctcaggat cctgcttgtc tcacttggat gtacttctct
     1861 gctgcagatc ccataagaga cacaaattct ggcctggtgg gcccgctgct ggtgtgcagg
     1921 gctggtgcct tgggtgcaga tggcaagcag aaaggggtgg ataaagaatt ctttcttctc
     1981 ttcactgtgt tggatgagaa caagagctgg tacagcaatg ccaatcaagc agctgctatg
     2041 ttggatttcc gactgctttc agaggatatt gagggcttcc aagactccaa tcggatgcat
     2101 gccattaatg ggtttctgtt ctctaacctg cccaggctgg acatgtgcaa gggtgacaca
     2161 gtggcctggc acctgctcgg cctgggcaca gagactgatg tgcatggagt catgttccag
     2221 ggcaacactg tgcagcttca gggcatgagg aagggtgcag ctatgctctt tcctcatacc
     2281 tttgtcatgg ccatcatgca gcctgacaac cttgggacat ttgagattta ttgccaggca
     2341 ggcagccatc gagaagcagg gatgagggca atctataatg tctcccagtg tcctggccac
     2401 caagccaccc ctcgccaacg ctaccaagct gcaagaatct actatatcat ggcagaagaa
     2461 gtagagtggg actattgccc tgaccggagc tgggaacggg aatggcacaa ccagtctgag
     2521 aaggacagtt atggttacat tttcctgagc aacaaggatg ggctcctggg ttccagatac
     2581 aagaaagctg tattcaggga atacactgat ggtacattca ggatccctcg gccaaggact
     2641 ggaccagaag aacacttggg aatcttgggt ccacttatca aaggtgaagt tggtgatatc
     2701 ctgactgtgg tattcaagaa taatgccagc cgcccctact ctgtgcatgc tcatggagtg
     2761 ctagaatcta ctactgtctg gccactggct gctgagcctg gtgaggtggt cacttatcag
     2821 tggaacatcc cagagaggtc tggccctggg cccaatgact ctgcttgtgt ttcctggatc
     2881 tattattctg cagtggatcc catcaaggac atgtatagtg gcctggtggg gcccttggct
     2941 atctgccaaa agggcatcct ggagccccat ggaggacgga gtgacatgga tcgggaattt
     3001 gcattgttgt tcttgatttt tgatgaaaat aagtcttggt atttggagga aaatgtggca
     3061 acccatgggt cccaggatcc aggcagtatt aacctacagg atgaaacttt cttggagagc
     3121 aataaaatgc atgcaatcaa tgggaaactc tatgccaacc ttaggggtct taccatgtac
     3181 caaggagaac gagtggcctg gtacatgctg gccatgggcc aagatgtgga tctacacacc
     3241 atccactttc atgcagagag cttcctctat cggaatggcg agaactaccg ggcagatgtg
     3301 gtggatctgt tcccagggac ttttgaggtt gtggagatgg tggccagcaa ccctgggaca
     3361 tggctgatgc actgccatgt gactgaccat gtccatgctg gcatggagac cctcttcact
     3421 gttttttctc gaacagaaca cttaagccct ctcaccgtca tcaccaaaga gactgaaaaa
     3481 gcagtgcccc ccagagacat tgaagaaggc aatgtgaaga tgctgggcat gcagatcccc
     3541 ataaagaatg ttgagatgct ggcctctgtt ttggttgcca ttagtgtcac ccttctgctc
     3601 gttgttctgg ctcttggtgg agtggtttgg taccaacatc gacagagaaa gctacgacgc
     3661 aataggaggt ccatcctgga tgacagcttc aagcttctgt ctttcaaaca gtaacatctg
     3721 gagcctggag atatcctcag gaagcacatc tgtagtgcac tcccagcagg ccatggacta
     3781 gtcactaacc ccacactcaa aggggcatgg gtggtggaga agcagaagga gcaatcaagc
     3841 ttatctggat atttctttct ttatttattt tacatggaaa taatatgatt tcactttttc
     3901 tttagtttct ttgctctacg tgggcacctg gcactaaggg agtaccttat tatcctacat
     3961 cgcaaatttc aacagctaca ttatatttcc ttctgacact tggaaggtat tgaaatttct
     4021 agaaatgtat ccttctcaca aagtagagac caagagaaaa actcattgat tgggtttcta
     4081 cttctttcaa ggactcagga aatttcactt tgaactgagg ccaagtgagc tgttaagata
     4141 acccacactt aaactaaagg ctaagaatat aggcttgatg ggaaattgaa ggtaggctga
     4201 gtattgggaa tccaaattga attttgattc tccttggcag tgaactactt tgaagaagtg
     4261 gtcaatgggt tgttgctgcc atgagcatgt acaacctctg gagctagaag ctcctcagga
     4321 aagccagttc tccaagttct taacctgtgg cactgaaagg aatgttgagt tacctcttca
     4381 tgttttagac agcaaaccct atccattaaa gtacttgtta gaacactgaa a
//
