LOCUS       NM_001102592            1662 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens HEN methyltransferase 1 (HENMT1), transcript variant
            2, mRNA.
ACCESSION   NM_001102592
VERSION     NM_001102592.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Reyimu,A., Xing,F., Zhou,W., Zheng,Y., Liu,B., Dai,J., Xing,Y. and
            Gao,J.
  TITLE     Screening of potential key genes in esophageal cancer based on RBP
            and expression verification of HENMT1
  JOURNAL   Medicine (Baltimore) 102 (49), e36544 (2023)
   PUBMED   38065897
  REMARK    GeneRIF: Screening of potential key genes in esophageal cancer
            based on RBP and expression verification of HENMT1.
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 1662)
  AUTHORS   Peng,L., Zhang,F., Shang,R., Wang,X., Chen,J., Chou,J.J., Ma,J.,
            Wu,L. and Huang,Y.
  TITLE     Identification of substrates of the small RNA methyltransferase
            Hen1 in mouse spermatogonial stem cells and analysis of its
            methyl-transfer domain
  JOURNAL   J Biol Chem 293 (26), 9981-9994 (2018)
   PUBMED   29703750
  REMARK    GeneRIF: methylation substrates in mouse spermatogonial stem cells
            and the substrate-recognition mechanism of mammalian Hen1
REFERENCE   4  (bases 1 to 1662)
  AUTHORS   Wang,H., Zhu,L., Duan,J., Wang,M., Yin,H., Wang,P. and Ai,S.
  TITLE     Photoelectrochemical biosensor for HEN1 RNA methyltransferase
            detection using peroxidase mimics PtCu NFs and poly(U)
            polymerase-mediated RNA extension
  JOURNAL   Biosens Bioelectron 103, 32-38 (2018)
   PUBMED   29277012
  REMARK    GeneRIF: The proposed PEC bioassay exhibited high selectivity and
            low detection limit of 3.36ng/mL for HENMT1 activity assay.
            Furthermore, the inhibition research indicated that chlorpyrifos
            could inhibit the HENMT1 activity with the IC50 value of 48.32nM.
REFERENCE   5  (bases 1 to 1662)
  AUTHORS   Hempfling,A.L., Lim,S.L., Adelson,D.L., Evans,J., O'Connor,A.E.,
            Qu,Z.P., Kliesch,S., Weidner,W., O'Bryan,M.K. and Bergmann,M.
  TITLE     Expression patterns of HENMT1 and PIWIL1 in human testis:
            implications for transposon expression
  JOURNAL   Reproduction 154 (4), 363-374 (2017)
   PUBMED   28676534
  REMARK    GeneRIF: HENMT1 and PIWI proteins are expressed in a
            germ-cell-specific manner and required for transposon control.
REFERENCE   6  (bases 1 to 1662)
  AUTHORS   Lim,S.L., Qu,Z.P., Kortschak,R.D., Lawrence,D.M., Geoghegan,J.,
            Hempfling,A.L., Bergmann,M., Goodnow,C.C., Ormandy,C.J., Wong,L.,
            Mann,J., Scott,H.S., Jamsai,D., Adelson,D.L. and O'Bryan,M.K.
  TITLE     HENMT1 and piRNA Stability Are Required for Adult Male Germ Cell
            Transposon Repression and to Define the Spermatogenic Program in
            the Mouse
  JOURNAL   PLoS Genet 11 (10), e1005620 (2015)
   PUBMED   26496356
  REMARK    Erratum:[PLoS Genet. 2015 Dec 29;11(12):e1005782. doi:
            10.1371/journal.pgen.1005782.. PMID: 26714033]
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 1662)
  AUTHORS   Simonis,N., Rual,J.F., Lemmens,I., Boxus,M., Hirozane-Kishikawa,T.,
            Gatot,J.S., Dricot,A., Hao,T., Vertommen,D., Legros,S., Daakour,S.,
            Klitgord,N., Martin,M., Willaert,J.F., Dequiedt,F., Navratil,V.,
            Cusick,M.E., Burny,A., Van Lint,C., Hill,D.E., Tavernier,J.,
            Kettmann,R., Vidal,M. and Twizere,J.C.
  TITLE     Host-pathogen interactome mapping for HTLV-1 and -2 retroviruses
  JOURNAL   Retrovirology 9, 26 (2012)
   PUBMED   22458338
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 1662)
  AUTHORS   Kimbrell,D.A. and Beutler,B.
  TITLE     The evolution and genetics of innate immunity
  JOURNAL   Nat Rev Genet 2 (4), 256-267 (2001)
   PUBMED   11283698
  REMARK    Review article
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BP378034.1, BC088366.1 and DB510959.1.
            
            On Nov 23, 2018 this sequence version replaced NM_001102592.1.
            
            Transcript Variant: This variant (2) differs in the 5' UTR,
            compared to variant 1. Variants 1 and 2 encode the same protein.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.263397.1,
                                           SRR3476690.175568.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000651461.1/ ENSP00000499017.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-51                BP378034.1         30-80
            52-1654             BC088366.1         1-1603
            1655-1662           DB510959.1         294-301
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p13.3"
     gene            1..1662
                     /gene="HENMT1"
                     /gene_synonym="C1orf59; HEN1"
                     /note="HEN methyltransferase 1"
                     /db_xref="GeneID:113802"
                     /db_xref="HGNC:HGNC:26400"
                     /db_xref="MIM:612178"
     exon            1..131
                     /gene="HENMT1"
                     /gene_synonym="C1orf59; HEN1"
                     /inference="alignment:Splign:2.1.0"
     exon            132..230
                     /gene="HENMT1"
                     /gene_synonym="C1orf59; HEN1"
                     /inference="alignment:Splign:2.1.0"
     CDS             210..1391
                     /gene="HENMT1"
                     /gene_synonym="C1orf59; HEN1"
                     /EC_number="2.1.1.386"
                     /note="hua enhancer 1 homolog 1; HEN1 methyltransferase
                     homolog 1"
                     /codon_start=1
                     /product="small RNA 2'-O-methyltransferase"
                     /protein_id="NP_001096062.1"
                     /db_xref="CCDS:CCDS787.1"
                     /db_xref="GeneID:113802"
                     /db_xref="HGNC:HGNC:26400"
                     /db_xref="MIM:612178"
                     /translation="MEENNLQCSSVVDGNFEEVPRETAIQFKPPLYRQRYQFVKNLVD
                     QHEPKKVADLGCGDTSLLRLLKVNPCIELLVGVDINEDKLRWRGDSLAPFLGDFLKPR
                     DLNLTITLYHGSVVERDSRLLGFDLITCIELIEHLDSGDLARFPEVVFGYLSPSMIVI
                     STPNSEFNPLFPSVTLRDSDHKFEWTRMEFQTWALYVANRYDYSVEFTGVGEPPAGAE
                     NVGYCTQIGIFRKNGGKATESCLSEQHDQHVYKAVFTTSYPSLQQERFFKLVLVNEVS
                     QQVESLRVSHLPRRKEQAGERGDKPKDIGGSKAPVPCFGPVFTEVEKAKIENSPTPFC
                     VGDKFFVPLQRLLAYPKLNRLCANEEMMRSVIADSIPLSSDGSAVVADLRNYFDEQFE
                     F"
     exon            231..359
                     /gene="HENMT1"
                     /gene_synonym="C1orf59; HEN1"
                     /inference="alignment:Splign:2.1.0"
     exon            360..472
                     /gene="HENMT1"
                     /gene_synonym="C1orf59; HEN1"
                     /inference="alignment:Splign:2.1.0"
     exon            473..607
                     /gene="HENMT1"
                     /gene_synonym="C1orf59; HEN1"
                     /inference="alignment:Splign:2.1.0"
     exon            608..787
                     /gene="HENMT1"
                     /gene_synonym="C1orf59; HEN1"
                     /inference="alignment:Splign:2.1.0"
     exon            788..965
                     /gene="HENMT1"
                     /gene_synonym="C1orf59; HEN1"
                     /inference="alignment:Splign:2.1.0"
     exon            966..1662
                     /gene="HENMT1"
                     /gene_synonym="C1orf59; HEN1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1632..1637
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HENMT1"
                     /gene_synonym="C1orf59; HEN1"
                     /note="hexamer: AATAAA"
     polyA_site      1662
                     /gene="HENMT1"
                     /gene_synonym="C1orf59; HEN1"
                     /note="major polyA site"
ORIGIN      
        1 gggcgtcagg gcggcgctga gttgaggacg ctggcggctc cgcgacgagc tttgtttttt
       61 cggttgggca tgctgcttac cgcaggatga tggaagcgac ggcccgtaca aagacagaag
      121 cgttggtttc actgagtcag tgctgattct ctcaaataga gcttgaagga taaatcttca
      181 tttttgtttc aacaaaactt cgaaacaaaa tggaagaaaa taatctacag tgcagtagtg
      241 tggttgacgg taattttgaa gaagttccca gggagacggc aattcagttt aaacctccac
      301 tatacagaca gcggtaccag ttcgttaaaa atttagtgga tcaacatgag cctaagaagg
      361 ttgcagacct gggatgtggt gatacttcac tcttaaggct gctaaaagtc aatccatgca
      421 ttgaattgct tgttggagta gatattaatg aggataaatt acgatggaga ggggattcgt
      481 tagctccttt cctgggggat tttctgaaac ctcgggatct gaatttgacc atcacattgt
      541 atcatggctc cgttgtggag agagactctc gtttgcttgg atttgacttg ataacgtgta
      601 ttgaattaat agaacatttg gattcaggtg atctggccag atttcctgaa gtggtatttg
      661 ggtacctgtc tccatccatg attgtcatca gcacaccaaa ctctgaattc aatcccctgt
      721 ttccatcagt gaccttaaga gattcagatc ataaatttga gtggaccaga atggagtttc
      781 agacctgggc tttatatgtg gcaaatcgct atgattactc tgtggagttt actggtgtcg
      841 gggaaccacc agctggagct gagaatgttg gatactgtac ccagatagga atcttccgga
      901 aaaatggagg aaaggcaaca gaatcatgtc tttcagagca gcatgatcag catgtttata
      961 aagctgtttt taccacctca tacccaagct tacagcagga aaggttcttt aaacttgtgt
     1021 tggttaatga ggtgtcccaa caagtggaaa gcttaagagt gagccacctg ccaaggcgga
     1081 aagaacaggc tggggaacgg ggtgataagc ccaaagacat tggtggctca aaggcccctg
     1141 tcccatgctt tggaccagtc ttcacagagg ttgagaaggc caagatagag aactctccca
     1201 cacccttctg tgttggagat aaatttttcg tacctctgca gagactcctt gcgtatccca
     1261 agttgaaccg cttatgtgct aatgaagaga tgatgagatc agtcattgct gactcaattc
     1321 ctctgagcag tgatggttct gcagtggtgg ctgacctgcg taattatttt gatgaacagt
     1381 ttgagttttg aaccatgttt atttcctgaa atttcagggt ctcagcgata gttgtgctca
     1441 cttagaattt agtttttttt gtgtaatcct aattcaagta atgtttttaa agtttcactg
     1501 caaaagtcta tgttccaagc cattggacag acctgcttga gatatggcca gactgcagtg
     1561 agccctgaga aagatatgag ggtttaaaac gggtgctttc ctttgatttt ggactttttt
     1621 gttttctcaa gaataaagaa gttggatgtg gtaatatgtt aa
//
