LOCUS       NM_016173              16993 bp    mRNA    linear   PRI 22-JAN-2025
DEFINITION  Homo sapiens HemK methyltransferase 1, mitochondrial release
            factors N(5)-glutamine (HEMK1), transcript variant 1, mRNA.
ACCESSION   NM_016173
VERSION     NM_016173.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 16993)
  AUTHORS   Fang,Q., Kimura,Y., Shimazu,T., Suzuki,T., Yamada,A., Dohmae,N.,
            Iwasaki,S. and Shinkai,Y.
  TITLE     Mammalian HEMK1 methylates glutamine residue of the GGQ motif of
            mitochondrial release factors
  JOURNAL   Sci Rep 12 (1), 4104 (2022)
   PUBMED   35260756
  REMARK    GeneRIF: Mammalian HEMK1 methylates glutamine residue of the GGQ
            motif of mitochondrial release factors.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16993)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   3  (bases 1 to 16993)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 16993)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 16993)
  AUTHORS   Hendrickson,S.L., Lautenberger,J.A., Chinn,L.W., Malasky,M.,
            Sezgin,E., Kingsley,L.A., Goedert,J.J., Kirk,G.D., Gomperts,E.D.,
            Buchbinder,S.P., Troyer,J.L. and O'Brien,S.J.
  TITLE     Genetic variants in nuclear-encoded mitochondrial genes influence
            AIDS progression
  JOURNAL   PLoS One 5 (9), e12862 (2010)
   PUBMED   20877624
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 16993)
  AUTHORS   Ishizawa,T., Nozaki,Y., Ueda,T. and Takeuchi,N.
  TITLE     The human mitochondrial translation release factor HMRF1L is
            methylated in the GGQ motif by the methyltransferase HMPrmC
  JOURNAL   Biochem Biophys Res Commun 373 (1), 99-103 (2008)
   PUBMED   18541145
  REMARK    GeneRIF: Human mitochondrial methyltransferase, HMPrmC, which
            methylates the glutamine residue in the GGQ tripeptide motif of
            HMRF1L, was identified.
REFERENCE   7  (bases 1 to 16993)
  AUTHORS   Bujnicki,J.M. and Radlinska,M.
  TITLE     Is the HemK family of putative S-adenosylmethionine-dependent
            methyltransferases a 'missing' zeta subfamily of adenine
            methyltransferases? A hypothesis
  JOURNAL   IUBMB Life 48 (3), 247-249 (1999)
   PUBMED   10690633
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA363235.1, AF172244.1, AK314023.1, BX537562.1, DB101409.1,
            DA404565.1, AC096920.2 and BQ183021.1.
            
            On Jun 2, 2019 this sequence version replaced NM_016173.4.
            
            Transcript Variant: This variant (1) represents the longer
            transcript. Variants 1 and 2 encode the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: HM005638.1, AF172244.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            MANE Ensembl match                 :: ENST00000232854.9/
                                                  ENSP00000232854.4
            RefSeq Select criteria             :: based on manual assertion,
                                                  conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-38                DA363235.1         371-408
            39-625              AF172244.1         9-595
            626-626             AK314023.1         616-616
            627-1600            AF172244.1         597-1570
            1601-2216           BX537562.1         1783-2398
            2217-2226           AF172244.1         2186-2195
            2227-2429           DB101409.1         298-500
            2430-2905           DA404565.1         121-596
            2906-3270           BX537562.1         3088-3452
            3271-4352           AC096920.2         130021-131102       c
            4353-5762           AF172244.1         4323-5732
            5763-5818           BQ183021.1         16-71               c
            5819-16993          AC096920.2         117380-128554       c
FEATURES             Location/Qualifiers
     source          1..16993
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p21.31"
     gene            1..16993
                     /gene="HEMK1"
                     /gene_synonym="HEMK; MPRMC; MTQ1"
                     /note="HemK methyltransferase 1, mitochondrial release
                     factors N(5)-glutamine"
                     /db_xref="GeneID:51409"
                     /db_xref="HGNC:HGNC:24923"
                     /db_xref="MIM:618609"
     exon            1..53
                     /gene="HEMK1"
                     /gene_synonym="HEMK; MPRMC; MTQ1"
                     /inference="alignment:Splign:2.1.0"
     exon            54..455
                     /gene="HEMK1"
                     /gene_synonym="HEMK; MPRMC; MTQ1"
                     /inference="alignment:Splign:2.1.0"
     CDS             228..1244
                     /gene="HEMK1"
                     /gene_synonym="HEMK; MPRMC; MTQ1"
                     /EC_number="2.1.1.297"
                     /note="isoform 1 is encoded by transcript variant 1; HEMK
                     homolog 7kb; m.HsaHemKP; testis secretory sperm-binding
                     protein Li 225n; HemK methyltransferase family member 1;
                     MTRF1L release factor glutamine methyltransferase"
                     /codon_start=1
                     /product="MTRF1L release factor glutamine
                     methyltransferase isoform 1"
                     /protein_id="NP_057257.1"
                     /db_xref="CCDS:CCDS2830.1"
                     /db_xref="GeneID:51409"
                     /db_xref="HGNC:HGNC:24923"
                     /db_xref="MIM:618609"
                     /translation="MELWGRMLWALLSGPGRRGSTRGWAFSSWQPQPPLAGLSSAIEL
                     VSHWTGVFEKRGIPEARESSEYIVAHVLGAKTFQSLRPALWTQPLTSQQLQCIRELSS
                     RRLQRMPVQYILGEWDFQGLSLRMVPPVFIPRPETEELVEWVLEEVAQRSHAVGSPGS
                     PLILEVGCGSGAISLSLLSQLPQSRVIAVDKREAAISLTHENAQRLRLQDRIWIIHLD
                     MTSERSWTHLPWGPMDLIVSNPPYVFHQDMEQLAPEIRSYEDPAALDGGEEGMDIITH
                     ILALAPRLLKDSGSIFLEVDPRHPELVSSWLQSRPDLYLNLVAVRRDFCGRPRFLHIR
                     RSGP"
     exon            456..547
                     /gene="HEMK1"
                     /gene_synonym="HEMK; MPRMC; MTQ1"
                     /inference="alignment:Splign:2.1.0"
     exon            548..641
                     /gene="HEMK1"
                     /gene_synonym="HEMK; MPRMC; MTQ1"
                     /inference="alignment:Splign:2.1.0"
     exon            642..776
                     /gene="HEMK1"
                     /gene_synonym="HEMK; MPRMC; MTQ1"
                     /inference="alignment:Splign:2.1.0"
     exon            777..841
                     /gene="HEMK1"
                     /gene_synonym="HEMK; MPRMC; MTQ1"
                     /inference="alignment:Splign:2.1.0"
     exon            842..891
                     /gene="HEMK1"
                     /gene_synonym="HEMK; MPRMC; MTQ1"
                     /inference="alignment:Splign:2.1.0"
     exon            892..997
                     /gene="HEMK1"
                     /gene_synonym="HEMK; MPRMC; MTQ1"
                     /inference="alignment:Splign:2.1.0"
     exon            998..1093
                     /gene="HEMK1"
                     /gene_synonym="HEMK; MPRMC; MTQ1"
                     /inference="alignment:Splign:2.1.0"
     exon            1094..1207
                     /gene="HEMK1"
                     /gene_synonym="HEMK; MPRMC; MTQ1"
                     /inference="alignment:Splign:2.1.0"
     exon            1208..16993
                     /gene="HEMK1"
                     /gene_synonym="HEMK; MPRMC; MTQ1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1479..1484
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HEMK1"
                     /gene_synonym="HEMK; MPRMC; MTQ1"
                     /note="hexamer: AGTAAA"
     polyA_site      1503
                     /gene="HEMK1"
                     /gene_synonym="HEMK; MPRMC; MTQ1"
                     /note="major polyA site"
     regulatory      5627..5632
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HEMK1"
                     /gene_synonym="HEMK; MPRMC; MTQ1"
                     /note="hexamer: AATATA"
     polyA_site      5648
                     /gene="HEMK1"
                     /gene_synonym="HEMK; MPRMC; MTQ1"
     regulatory      5743..5748
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HEMK1"
                     /gene_synonym="HEMK; MPRMC; MTQ1"
                     /note="hexamer: GATAAA"
     polyA_site      5762
                     /gene="HEMK1"
                     /gene_synonym="HEMK; MPRMC; MTQ1"
     regulatory      9850..9855
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HEMK1"
                     /gene_synonym="HEMK; MPRMC; MTQ1"
                     /note="hexamer: GATAAA"
     polyA_site      9877
                     /gene="HEMK1"
                     /gene_synonym="HEMK; MPRMC; MTQ1"
     regulatory      13283..13288
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HEMK1"
                     /gene_synonym="HEMK; MPRMC; MTQ1"
                     /note="hexamer: AATAAA"
     polyA_site      13308
                     /gene="HEMK1"
                     /gene_synonym="HEMK; MPRMC; MTQ1"
     regulatory      16976..16981
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HEMK1"
                     /gene_synonym="HEMK; MPRMC; MTQ1"
                     /note="hexamer: AATAAA"
     polyA_site      16993
                     /gene="HEMK1"
                     /gene_synonym="HEMK; MPRMC; MTQ1"
ORIGIN      
        1 agttcggggc agcttctccg gctggtgggt gggtggggca gcctttcagg cagggtggca
       61 accaactata tctgaggacc agagccattt tggggcacca gagcttgtga cctctccatc
      121 tccacccagc tgggtccagg ggccactctc agcactcacc tcagcagctg acatcataaa
      181 gcagacttgg gaacctggaa gcactctgga gaacctttcc ctgagacatg gagctttggg
      241 gccgaatgct gtgggccctc ctgtctggcc cagggaggag gggaagtacc cggggctggg
      301 ccttcagctc atggcaaccc caaccacctc tggctgggtt atccagtgcc atagaactgg
      361 tcagccactg gactggggtc tttgagaaga ggggtatccc tgaggcccgg gaatccagtg
      421 agtacatcgt ggctcatgtc cttggagcca aaacatttca gagcctgagg ccggcacttt
      481 ggacccagcc cttgacctct cagcaactac agtgtatccg ggagctgagt agccgtcgat
      541 tgcagaggat gccggtgcag tacatccttg gagagtggga cttccagggg ctcagcctaa
      601 ggatggtgcc cccagtgttt attcctcggc cagaaacaga ggaactggtt gagtgggtgc
      661 tggaagaggt ggcccagagg tcccatgctg tgggatcccc aggcagcccc ctcattctgg
      721 aggtgggctg cggatcagga gccatctccc tcagcctgct gagccagctc ccccagagcc
      781 gagtcattgc tgtggataag cgggaagctg ctatctctct gacccatgag aatgctcaga
      841 ggcttcggtt gcaggacagg atttggatca tccacctcga catgacctca gaaaggagct
      901 ggacacacct gccctggggc cccatggacc tgattgtcag caaccctccc tacgtcttcc
      961 accaggacat ggagcagctg gcccctgaga tccgcagcta tgaagacccc gcggccctgg
     1021 atggtgggga ggagggcatg gacatcatta cccacattct ggccttggca ccccggctcc
     1081 tgaaagactc tggtagtatc ttcttagaag tggacccaag gcacccggag cttgtcagca
     1141 gctggcttca gagccggcct gacctgtacc ttaatcttgt ggctgtgcgc agggacttct
     1201 gtgggaggcc ccggttcctg catatccgga ggtctgggcc atagcatggc tgccctgtgg
     1261 atgccttgtc agtgccgcca gcctgaccag aggggaggtg gatggcactt tccagagccc
     1321 aggttcttat ggcatttccc agggttctgt gatttcccca tgctctgcat ttctaggata
     1381 tttctaggac acctggattg gctccatcac atcagagtgg ctgagggcag ttgctctgtg
     1441 ttggtgaaat tgctgtgggg gtatcggggg atatggccag taaagtattg agagactaac
     1501 aaatggtgac ctaatgtttt gtccatgact tgcaggtccc ctgaccccct tactcccagg
     1561 tagcactggg gcaagggttt ccttctgccc cagcagggct ggccgtcagt cccctgcttg
     1621 gtagtggtgt gggggtgcag tgtggaggaa ggcacgtgag tcctcactcc tggccttgga
     1681 taccatgggt cctggcatag agcagctcac tcccagggat tgattagtcc tccactgccc
     1741 tgggtgcatg cgtacacaat tccctggcca agcctggctc gagcacagga agctcatctg
     1801 cgttttggct caaggatgac tgcctgcttt ctggagggga gggtctggag gtctttgctg
     1861 cacagttcct gggtcgcaca tccacgttca tttaactgaa ggcttgagcc agtgaggggt
     1921 gtttcctttt tatccccata gcttttagct aaaacatccc tcccgagttg accccctggg
     1981 gtttcaaata acccatgtgt ccctggttgg ggctggggag agtgagaagc tgagatactg
     2041 ggcacagggt tgtggcctcc accccagctc tggtctgtgc agactcatgg ccaccaggag
     2101 gcctgcagat ccagccttcc tgtcaacagc gacaggaaat ctctaggttg gtgagtgctg
     2161 gtgatgtgag cctacatcag ggtgggtcct aagaaacatg gcaaaccagg ctgtctcatt
     2221 ccactagact gccccctgcc accctggcac ttcccagggc ctggcagtat ggtctgatgg
     2281 gcagtatggt ccaataggca gcatcctctg ctgcagctgg gagagctgag ttccagggct
     2341 gtgtcctgca gtgggacctt gggcaactcc tttccctatg agaagctggc tcttctgagt
     2401 ccagggccaa cgccaactgg caacctcttt actcttagtc aagtggaatg tgcatgctgg
     2461 catctgaatg tccattcgcc aggcatggag agcaagagaa ggtatgtact gcctgaggtc
     2521 acatgacagt gaccaagtgg agacagtaag ttagatccct ccctttgggg agcctatatt
     2581 gctggagtca tacccagcct aagtgttgcc ctgcactatg gctggaggac acatttggta
     2641 gaggtcacac tgcagctccc agtgccccag tgtcctgccc tgtgcccagc cccagctgca
     2701 tggactctga gctgcccctg gcttccttta aggaggctgc tccagaagga acctgggtgg
     2761 ggagggcgaa gggggtgcac aaccagggca aggctcccca cttccttagt cccccatgct
     2821 cacagacctt tgcctgctaa ggtcctcacc agtattgccc tttctgtctt tctccttgtg
     2881 ccctttggct cttgctgtct tcagcagcat ctcagggtag ctgccctgac ctcggagcag
     2941 tctgtcgccc ccctacacct cagccagtcc tggcttccct gatggtctct ccctcctggc
     3001 ctcaggccca ttcctgagga agggccttgg cgagcttgtg gatgttgcac cagaagagag
     3061 tgcagtgttg gagagtgaca ctgtcggggc agctggggcc acaagcagga gccggcctcg
     3121 ggcacaactt tctgcccaga aaaatgtgca gcttgactct gctgaggaaa aggtccaagc
     3181 caagaggact ggcaggcggg gcctcaagcc tgcagccact ggcttgattg ggccctggac
     3241 gttgagccca gatgttggag ccacaccagc ctggatttca atcccagaat ctgcccctca
     3301 cgaggatgtg accttgggca gatgacttca cctcactcag ccttggcttc taaggctgag
     3361 aaatgggact taatgcttta ttttatagga tgcatgtgag gagcccatgg gatgtgcctg
     3421 gcttggcaca ttgtggcatt tttccttgcc ttcctcggag ggcagacaca gggaggaagg
     3481 acccagtgcc ctcaggcgtc catctgatgc atgggaccaa cataaggcag gcagggatac
     3541 aaggcagtct ggaaagaagg gaaggcagga gtttcagtct tgggctcttg actcctcact
     3601 gttgtctaga gatggagcca gcaggctggt agcctggcag cctacatctc ccctcagcct
     3661 ctcctcacta tggccccagt gccttgaggc ccaggccagg gcagccagtg gctctagctc
     3721 agggaaagcc aggcccacct gccctatccc ctcccttgct cctgaggcca aagccagaga
     3781 ctcgaacagc ctccccacca ccaccagcat atgtcaagga gcacttgcag gcagaatggg
     3841 aggaggacat ggagctgatg gagtccaggc tgtgcaagcc cctgaggtct tgagagatgt
     3901 gcccactgcc cgtgcagcct ccttcagcca gagcccagag catagacagg agtgtaggag
     3961 tccctgtttg atgtactctg ggagagtaat tctatctcct cttctgatag ttggggaaac
     4021 tgaggccttg tctcacagtt ggatgctttt cccagttgtc agtgggtttc tccatgggtc
     4081 tcatacagct gccttattga aataggcccc gaacccccta aatgcaaaaa atactctttt
     4141 ttgctccttt acccccacct ggaccctggg ctattggctg ctcccaatcc ttgccccaaa
     4201 cacttagctg gctccccatg acttaagtgt gttctcttgt gtcctatgga atccagttct
     4261 gaagaggtgg gggaggacaa ctgtgggaaa agccctgggg gcccctccca aggccccatc
     4321 agtgctctga gtaggctgtc atcagaacaa agggctccac tgctgacaag gtttgagaac
     4381 tgctggcttg aggtgagaac ccctttaacc tctgcgggac agcatgtctt tccctatcca
     4441 ccttcgattc ttttctcttt tttttcttca ttggctcctt cttagtggat tctcttctct
     4501 actgccctgg gcttcagcct ttgtgcagta ctctcgatgc cctgaacaca caccttccct
     4561 ttgcccaggc ggtgcaaaca atccacttct tcaagctcca acacaaatgc tgcctccttt
     4621 aggatgcctg ctctgtgctc tccctgcctc ccctagccca tacctctgct ggcaccttct
     4681 gtaccatgcc ttcagaaacc ttcttatccc cctcatctct ggggccccct gtggatctgg
     4741 catacccaag ttcagtaaat gtctatcagt aagctgatgg tacatgcatt ttctagaata
     4801 gagctgggac ttcccatgtg gcccacatct gacctggcag cccatgtatt ccggtcatta
     4861 gggatgggaa gccatgagga cctggccttc tgcccgaccc aggcagccat tcaagttgag
     4921 caatggccac ttcgaagact caagtgcacc tgatccctgc gcaacagcca caccaggaga
     4981 acaggctgtc cttggcggca gtaggagcag gcgccaggtt tcctggagct cttggcttca
     5041 gccagccccc agccagagtc ctggctagga cagtgacctg atctcctcct catgaccttc
     5101 tgccctggac aagccccctg aactggattt gggactgtca aagcaactct acccctgctc
     5161 tggtaggctg aacagtgacc ccccaaaatg gcagtgtctt aatcacctaa accttaacat
     5221 gtgactatat taccttcaca tagcaaaatg gactttgcag atgtgattaa ggatcttgag
     5281 atggaaggag tatcctggat ttttcaggta aactgagtat aatcacaagg gcctctgtaa
     5341 aggaggcagg agtgtcagag tgacggaaga aaatgtatgt aacaatggaa gcagaggtca
     5401 gagtgatgca attgctggag gaagagccat gagccgagga atgcagacag cctcttctcc
     5461 tctggggcct caagaagaat gcagtcctgc caataccttg attttaagcc ctgtgaaact
     5521 gatttcagat tgctgacctc cagaacagta agatcataaa tttgtgttgt tttcacatgt
     5581 gtgaaaacac atgtgtgata atttgttaca gcagccacgg gaaacgaata tagattgtgg
     5641 tgcccaaatt agagtgctgc tgtaacacac gcctactgat tgaagtggct ttggaattgc
     5701 aacgtggaaa tgggcagagg ctggaagaat tttgagagtc atgataaatt gccttaacca
     5761 cctctcttct gataggtgat gtggccaggg gaactcttcc tcaaccttca gacctaaact
     5821 gcccaggccc cccctttttt tactcatatt tggagagagg tggccctttc ccagagtgag
     5881 aagactgaaa gactgaccag catgggtccc agaagggagt gggtgaggaa tgggactggg
     5941 atgtggggag aaagtgcaag gctggcctcc atggcccatc tccagagact gggtccagag
     6001 aggacctcag agactgggac caagcctgtt cctgctctcc tgtggagtta tgtgactggc
     6061 ccaggacccc ttgggtgtgt gaggatgagc aggggccctg tactctctgg gttgcaggta
     6121 ctgcatctgt gaaatgagca cagcagcccc tgactttgag ctaatgggcc tggcccctcc
     6181 atgtaagcca taggcctgag cagagctgtg tcaccattct gttcctatgg tggagtgggg
     6241 ctatccctgg ggttctcaac ctcctggttc ctaaaactga attggccaac tggtgccctc
     6301 agccctgtcc ctgtccaagc tgggactagg acctcccttt ccaggggtac taaaggactc
     6361 tggagacacc atcccaccca gccaaggctt ggggctcctg aagccagtcc taacccagga
     6421 gccacatgga gagctactgg gaggccagac atgggcctca tgctgggttt cagagctgag
     6481 aagctggggg acagagagca tggacacctg ggttcaaggg tcagcttggc cccaaatcac
     6541 ctggatacgc tttggatgaa gccaggaggg cctgtttata ctggaagggg tggatcatgg
     6601 caagacttat attcaatccc tgatcttttt tcccagagag ggatggggct tacttgaagt
     6661 aacacagcac ttgactcctg actcccagta tgatgctctc atctcctatt gcttccaaag
     6721 gcctggggtt agttaattaa gtcaattagg tggtgctcat atggctgtcc atgtagctac
     6781 taaacccagg gattctggcc cataaagccc tctgggccag gtttatgggc aggtaagagt
     6841 cacattaggc caactgtagc cctgggtgtg cagacagatc tgagtcccat ctgaagctct
     6901 ggtagctgtg ggaggagagc aggatttccc ccgcttgcgg aatgagtggg tgccttcccc
     6961 tctgaggtaa ttggctgtac agcccccagc ctctgccccc cagggccctc ctgaggtgtc
     7021 tggaaggacc tttgggatct ttggcctcat aaccagaaat cagaagcttc aaattctaga
     7081 ggcccctttt cttccaaaga ctacctctca cacatacagc agtcctgcag gaaaatggtc
     7141 aggaaaggct aattttacag ctcacaaagt gggaaaggca gggaagccta cctcagcttc
     7201 atggaaagcc ctgaactggg ctgacctcca cctgccccag ctatgcctaa agggggaggg
     7261 tgagacagct tagagtagcc atgccccgtg gcaggcaggc ctggctaagt ggcccccttc
     7321 ccaggggctg gggtcttccc caaacactct acctctcaga gcagaggatg tcaccttatt
     7381 gctccctctt gtactaggtc tgcggccctc ttctggggca tgtggctggg gaaatggtgg
     7441 gatcagatat cccctactga gcccagggag cagattgttg gtgcctgaag cgctcacaga
     7501 gatccctgtg gtacagatga ggaaactgag gcttagcagg acaggagcag ggctggggca
     7561 cgagccaggt ctcttctctg gctcacagct gccagtcctt cctttgccac ctgaagaccc
     7621 cccaccccac ctggctttcc aggaacaggc ctgcctgcct ggagaggcgt ccaagtccct
     7681 ctgctgttcc tggaagtcag tgtgaggctg agctacgtga ccaagcaaag caaactagga
     7741 ctacaggtgt gaacaaccct gcctggctaa tttttaattt tttttttttt ttagaaacgg
     7801 ggtcttgcta tgttgccagg gctggcacat ggggttttta gttgtcccca cattacaaag
     7861 ttgaggacac caaggctcag cagggtgcag ttgcaggtca ggttgcatag ctggacagga
     7921 agggaggatg aggctgccat tggtgtccat ttgactatca gagacgctga ggccagcata
     7981 actaggtcct gggctgtccc ctgcagagct tagcgcagaa gttacctggg cttgtggacc
     8041 cttggagcag gagcaagggt ttccagggct gtccctagag ggctttctag aagacctgca
     8101 gctgctcctg ggtgcatctc ctgaggtata gggccagggc ctcagtctct caagacctac
     8161 tttgtgtcag gcaccaggga gatgatacca agtggagggt cctatagaat atggtgcatc
     8221 ctgcgccaag tacttccagg attatcttac tcaaccttca tgacatcctc agagatggag
     8281 gctgtgaacc ctctcgttat actgacgaag aaacaaactg gggctaagaa acttgctcat
     8341 aggctggctg agctgtgatt ctggcatagg ctgagtgacc tggagctgga ggccttagtc
     8401 atcacaccag gcttgaggag gggcggatag gacaggaata taccaggcag tagggacagt
     8461 ctggacaaaa gcctggagtt ctgcagacat gcgggtaggg atatgtaggg gtgaggtggt
     8521 gccgagaggt ccccttggct gagctctgaa tgcatttctg gggaccaggg gcctaaagag
     8581 ctttttgagc tgagttacag ggttagagaa gcattgaggc tgctctttct ggctgccagg
     8641 aaaaaggggc tggggcagga ggctgcaact tgtccagggt ctggaggtgg ctggggtctg
     8701 tagcagtggc atcagatatt ctggaacaga actgactcac aaccattgct gagggtttgg
     8761 atgtgagatg gccaggacca cagtgggccc agggatctga ttgggacctg ggggaaatgg
     8821 ggccaccctg agttggagac tggggtgctg gggggaacag tcggggagct tgacagtgta
     8881 ggatgccttc agaatatccg agaagcagca ttggggcgtc tggagatgca aatgtgacca
     8941 tgtggggaca cagtccaaag gcaacccttg aggtagagtt gtgaagacca ctctcctaga
     9001 cctgagtgct ggcgacaaca gtaccgggca tctgcagtgt gtaaactagt gccaggtgct
     9061 gcacacacgt ggtcataaat ccatctgttg gcacatcagg gatcccttcc ttggcccact
     9121 gtatggatga agggactgag gcttaacatg cccaaggtca cacagccagc agctggccat
     9181 gctggagttt gaacctagtc ctctgacatc ccagtcagga gaggcgtgag caggctgccg
     9241 aggccaaatg cagcccaggg cctcaacccc ccagaggggc ctcaggagcc agttaggtgc
     9301 cccacagcca acgtgggctc cagggtgaag gggaaagtga tgaatcaaac agaaagcagc
     9361 agacgcagac tgagctctct ccctgtcggc ccgggtgcca gattttcacg cccacagcaa
     9421 aggggccagg cagagcgcta ggtgtggggc aggagagggc agcgaaagtg gctgccactg
     9481 aacccagact ctgatgtact ccggatatgg gtggcgtgga ctgacagaca cccagcccag
     9541 ttccagactg aaagttgagt ctgtctcagc atgtgaggat atgaagtttg ccttgtcatg
     9601 gggagcattt caggtcccta ggcctgtgag gttctgggat gaggcaccag atggatgtgt
     9661 ctaaaaaccc ttgttgaagg cactccacag ctcaataact gaccattgct cactactctc
     9721 ttggcccgca ctgaaagcca ctgtttcttc atcatttcac tcagcccgcc tgtctcagcc
     9781 ccccagaact ttggagctca ttctttcaaa gtacatttca tgtgcaactg aaatacatat
     9841 ttttaaaaag ataaaatgag catctagtta gcaaaaatgt attttgcaag tgcaaattct
     9901 ttcttagcat acagtccatc agggcgaaga gcaatgaaca caaatgtttg aactgggggc
     9961 tgggatggta gttgtagtct tatgttggtc tcaatgtcca ttttctttca gtgtttgatt
    10021 ttggtagcac aggacaaagt gaccgctgat tctcacagat aattgtttac aaatgctagc
    10081 actgtgtctg aagcccacct tgcagtctct ttgattgaca gggacagcaa gagaagcctt
    10141 atctcaagct cagcacaaac cctgttctct gagcacatag gaaggattct aatgtgtcat
    10201 aatttggtgc tgtgcccatt tgagtctgtg tgtggttttt aaaattgggt tttttttttt
    10261 tcaataatta aaaatatgag ctgggcatgg tggcacatgc ctgtggtccc acctactcgg
    10321 gaggctgagg caggaggatt acttgagcac aagagtttaa gacagcagtg agctataata
    10381 gcgccactgc actccagcct ggacatcaga gtcagaccct gtctctaaaa aaataatttt
    10441 aggccgggcg cggtggctca cgcctgtaat cccagcactc tgggaggctg aggcaggcag
    10501 atcacctgag gtcaggagtt cgaggccagc ttggccaaca tggtgaaaat ccgtctgtac
    10561 taaaaataca aaaattagcc gggtgtggtg gctcacgcct gtaatcccag ctacttgaga
    10621 ggctgaggca ggagaatcgc ttgaaaccga gaggcagatg ttgcagtgag ccaagatcac
    10681 gccattgtac tcccacccag gcaacaagag caaaactctg tctcaaaaat aataataata
    10741 ggccaggcgc agtagctcac gtttgtaatc ccagcacttt gggaggccaa ggatcacaag
    10801 gttaggagtt caagaccagc ctggccaaca cagtgaaacc ccgtctacta aaaatacaaa
    10861 aattagctgg gtgtggtggt ggacacctgt aatcccagct actcgggagg ctgaagcagg
    10921 agaattgctt gaacccggga ggcagagatt gcagtgagcc aagatcatgc cactgcactc
    10981 cagcctgggc gacagagcta gactccatct caaaaaaaaa aatttaaaaa taataatttt
    11041 aaaaaaatgt tttaaaaatg aaagaaaagg aagaagggag gaggaagcca gaaagaaagg
    11101 ggccgtgcag ggtggctcac acttgtaatc ccagcacttt gggaggctga agcaggcaga
    11161 tcacccgagc tcaggagttc aagaccagcc tggccaacat ggtgaaaccc tgtctctact
    11221 aaaaatacaa aaattagctg ggcatggtgg ctcatgcctg taattccagc tactcgggag
    11281 gctgaggcag gagaatcgct tgaacccgga aggcggaggt tgcagtgagc tgagatcacc
    11341 tcactaccct ctagcgtggg cgacagcaag actgtttcaa aaaaaaaaaa gaggaagagt
    11401 gaaaggatgg acgaaaggaa ctcctgtggg gtggggcatc tggggcactt gatgccccac
    11461 gtgaccattc ccttcccgga ggtctgcccc aactgctcca gcccccatag cctgaggctc
    11521 ccggcacctc ccacccagca tctgtcacag caaatcaatc tgggccacat gctcctgaaa
    11581 ggtccttggg agccccacca ggctccagtg tggttgcccc aagtactcca tgagtagaca
    11641 tgggctaggg acttacccag gcccttggtg gtgcctccaa gggtggaaag actgggggac
    11701 cctgtgcccc tgaaagccca gagtttcact gtttctgggt accttcattt ctgggaacta
    11761 actgtgcttt ggaaagagag aaatgagaag caaaacccta gggccccagt atcacccctc
    11821 ctttctgtct tccttccgct tagtcacagc caagagctca gtacctcagc ctcccttagg
    11881 gccactaggt ggggcagaat acaaggtcag gcgaggacac agcatctggg acgagtcctt
    11941 tcctgccaac agcctccgtg aggggtccta ggattgctga gggctgagac caccttccca
    12001 aagttgggga aggggtaact gaggctgtca tctggcctgt ctcacctgtc ttacctgcag
    12061 gcccatcatt cctgcacagt catttattag gcaccaactg taagccaggc ccaggaggtt
    12121 ggatggttca ggtggggccc tggccttgca gaactcatgg acttgcacat agactcctcc
    12181 tgacaggccc ctcatgagta gctgggtcct tacgattttt ctggtcagca gtggggagcc
    12241 gtgggaggca ttaggctctg gggaagccaa gcaacatcac atgtacacag catagagact
    12301 cacacaccct tgcacaggca cccacacgta cgtgtatgtt catgggcgtg ggccttgagc
    12361 accgggtccc gtgagccact gcacctggcc aactgggtcc atgtccataa tcataggtgt
    12421 ccacgcaggg cctcccttgg atgggggagc tccaggctct ggaaaccaag ccagggttgc
    12481 atgcatgcac gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtttgtgt gtgtgtggtg
    12541 ttactctgcc tcagaggccc aaccactgcc tccaagtcat gggagcgcag agcccatgta
    12601 agctcaaaca ctgccccatg tcatagaaaa gagggccaaa gatggggctg ggcgtggtgg
    12661 ctcactcctg taatcctagc actttgggag gctgagtcgg gtggatcacg aggtcaggag
    12721 atcgagacca tcctgactaa cacggtgaaa ccctgtctct actaaaaata caaaaaaatt
    12781 agctgggcgt agtggcgggt gcctataatc ccagctactc aggaggctga ggcaggagaa
    12841 tggcctgaac ccgggaggcg gagcttgccg tgagcggaga ttgcaccact gcactccagc
    12901 ctggacgaca gagtgagact ccgcatcaga aaaaaaaaaa aaagaaagaa aagagagcca
    12961 aagctcagag agggcccatg acttgcccag tgtcacacag caaggtgagg caggtctggg
    13021 agggaaatga accagacctt atccacatcc ctgacctttt tggcttcatg cttggagctt
    13081 ggcaccccct gagaaccaca ctccgttgac ctctgggaaa tggaggctgg tgattctccc
    13141 agcaaacccg cctgggaact aaccacaggc tcctcccagc tttcactaac caaccccagg
    13201 atttccaata ccggtgcttt gcttagctgt actgggcacc tggaactcat tctccctgcc
    13261 accctccctc cctgattcct tgaataaaat tcagccttcc aaattttact caaatgcttc
    13321 tactttctct gaccctctgg aactttcccc tgttgccagc ctccctccag catctgtccc
    13381 ctcattccca cctcctgttt acttccctac aaaagccaaa aattgacccc tagagcctga
    13441 gactctggat ggaggatctt cgtagctggg gccacaccca ggccagccca gcccttcaga
    13501 ctctgccagt cacagtgggg tgggccctgg agctggaaga ggaaacagag cctctgccag
    13561 agtcaccacc tgcccccaac cctacttctc ctccgaaggc ccaacaaggt cctgtgcgcc
    13621 ctccaagggc agaggaggct gctggctgtg gctgtgggag gagacctgga gcactgtgcc
    13681 cgaggcaaag ctggccccgt ggagtttgct attccagctt ttatttactc ctctgtaccc
    13741 tgagcaccat caactgggtg ccaggcctgt gcctatattt ggcagggtga ggggtggggg
    13801 ccagacaaat tgtagaggag cctcaggtct ttggggtgtt gcccctccac tacatggccc
    13861 cagaccaagt tctctgccag tttgcagagc tcttcccttc cacttggtct tgaggagggg
    13921 ccagagaggc tgcctttacc ccatgagctc tgaaccctct cactatctgg ctgtcccctc
    13981 attctctcac cacccactcc cactcccgat gggagcctca ggcctggctt aggaaccctg
    14041 cccacagctg tactaatgcc cttggctttg ctatagggaa agaacacagc cactccacac
    14101 tctgggacag ccagcttgcg tccatcccct agtgcagagg gctagtttag ggaaggacat
    14161 taggaccctg actgtgcacc tgactcaggg gccccggaga agatttggtg ggctaagggt
    14221 gggcaggaga atacaactca cagccaagcg gaaggtggct ttcagctccc cccttctcat
    14281 tttttcttac tgtggctgca ccagggctgt gcccctcatg gaaggttcac tttcctgctt
    14341 atctagtctc agatgttaca gtttttcttg ttatgtatta tacagagaga tggcctaact
    14401 ggttcttgct gctgcatggg caagccatta atttacaggg gtttatctta taactggaca
    14461 cctttttgtt ttctcttaca agatgtgctt gttgttgctt ctcttggatt ttctacattg
    14521 acattttacc actgaaaata atgacaatga caaatttgtc ttttcctttc tttctttttt
    14581 ttttttttga gacaaagtgc cactctgttg cccagactgg agtgcagtgg cgcgatctcc
    14641 caggttcaag cgattctctt ggctcagcct cccgagtagc tgggattaca ggcgtccacc
    14701 accatgccag gctaattttt tttttgtatt ttcagtagag acggggtttt gccatgttgg
    14761 ccaggctggt ctcgaactcc tgacctcaag tgatctgcca cctcggcctc ccaaaatgct
    14821 gggattacag gcctgagcca ccacacccgg cctgtctttt cctttttaat acttatggct
    14881 cttatttatt ttccttgttt aatagcttgg ctgagacctt caatacaata ttgaaaaact
    14941 gtaacgacta gagcatttgt atattgtttc tcacttgggg tgcctctggt ccctccctgt
    15001 caagtgcact tgctattgat tttggataga tggccctttt ctacttaaaa atggttcagt
    15061 ctagttctgg ctgactctga ttttttgttt attttagagg cttttttttt catctttcat
    15121 tcatgcaaca gaccttcagt gagctgattg ccaggactac actgtcatac tgtggtacca
    15181 aagaaaaatt gacacggtcc ctactcataa ggagttcatg gcctggtgga ggatacaggc
    15241 aggcaaatag atccttatcc ttaggccatg gggcatgcat tcagtcacca tctgctggca
    15301 ctgtgccagg cacaaggaat gggcactatt caggcaggcc cccatcacag tctggtgggg
    15361 tccaagcagt tcacatcccc tctcactcaa gttcccacag agtagttgca atggtgagct
    15421 gaggttttct ctgctgcaga ggccaggtgg gtaagtaacc aaaagtgcct gagagctaat
    15481 cccctggagc agcctgaaac caaggaagac agagagttgg tagacacatg cccagctccc
    15541 tcaccccttg ggtgggacaa ccgtggcttg tgttttctgc cagctcccaa ctgaagactg
    15601 agcctcagtt gcctacagaa ggaacccact cataaaccca cctacctcct ttggcttcct
    15661 ttccttccct gtctcacccc ctctcccatt ttatatcaat aagtgtgaag gctgagattt
    15721 tatccgactt tctttttttt tttttgagac agagtctcat tctgttgcca ggctggagtg
    15781 cagtggcgtg atcttggctt actgcaacct ctgcctccca ggttcaaacg attctcctgc
    15841 ctcaggctcc cgagtactgg gactacaggt gcgcaccacc acacccagct attttgtatt
    15901 tttagtagaa acagggtttc accatgttgg ccaggatggt ctcaatctct tgacctcatg
    15961 gtctgcccac ctcggcctcc caaagtgctg ggattacagg agtgagccac cgcgcccggc
    16021 ttatccgact ttgaagcaaa caagttagtc tatcatagtc tatcatattc tcaaggatca
    16081 tggcagaagg cacagaactc tgggttcaga aacagacaac agattattgt ttacagtaac
    16141 ggcagtagac agagtaaaag cattttcttg caccacttcc ctgaaccgca gttcctacag
    16201 agtgatgtga tgatggcccg gtgatgcctg catatgcatt gggttgcact acggtggaga
    16261 aactgcatgc tgaatttagg ggacccacgt cttttataat aagctgcaag caaacctgcc
    16321 caggtgggag atgttatctt tagtatacgg gacaataaaa tctgcctttt gctctggagg
    16381 gagatactac ctctatcttc taaggttact cactataata atcacccttg taaagattat
    16441 ttagaacaag ggcaaccagt gcctctactg gcaagtgtgc agaaatagga gagaaccatg
    16501 gggaattgtc tctcaatacc ttactggtgc ttcttggcat tacttcccaa ataatctatt
    16561 tgcatttgga tgcttgtctc agagtggttt ctgcaggaaa aaacccaaga tagagagaca
    16621 acactatata ggcatcgatg tatttctggg gtgactctct ttcctccaga cctctcctgc
    16681 aaacaacgca ggggagccac gcaaaggaag aggtttccac acctgggatt gatactgctg
    16741 ggactccagg gcaggtgctc taaaggactc ttagaatttc atttttaatg tttaatattt
    16801 ggtccatcta gaatttatgg tggaaggtgt gaggtagacg ttgcactttt ttttccttcc
    16861 aaattgctcc aatatcactt actggaaatt gtgctttgtc tgttaccact ctcttctaat
    16921 ctgcctgtgg cctgttcctt agagtgtatc acaaacatac aagtgatcaa tttccaataa
    16981 attgattaaa gaa
//
