LOCUS       NM_001037335           10002 bp    mRNA    linear   PRI 10-FEB-2025
DEFINITION  Homo sapiens helicase with zinc finger 2 (HELZ2), transcript
            variant 1, mRNA.
ACCESSION   NM_001037335
VERSION     NM_001037335.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10002)
  AUTHORS   Huntzinger,E., Sinteff,J., Morlet,B. and Seraphin,B.
  TITLE     HELZ2: a new, interferon-regulated, human 3'-5' exoribonuclease of
            the RNB family is expressed from a non-canonical initiation codon
  JOURNAL   Nucleic Acids Res 51 (17), 9279-9293 (2023)
   PUBMED   37602378
  REMARK    GeneRIF: HELZ2: a new, interferon-regulated, human 3'-5'
            exoribonuclease of the RNB family is expressed from a non-canonical
            initiation codon.
REFERENCE   2  (bases 1 to 10002)
  AUTHORS   Luqman-Fatah,A., Watanabe,Y., Uno,K., Ishikawa,F., Moran,J.V. and
            Miyoshi,T.
  TITLE     The interferon stimulated gene-encoded protein HELZ2 inhibits human
            LINE-1 retrotransposition and LINE-1 RNA-mediated type I interferon
            induction
  JOURNAL   Nat Commun 14 (1), 203 (2023)
   PUBMED   36639706
  REMARK    GeneRIF: The interferon stimulated gene-encoded protein HELZ2
            inhibits human LINE-1 retrotransposition and LINE-1 RNA-mediated
            type I interferon induction.
            Erratum:[Nat Commun. 2023 Jan 30;14(1):493. doi:
            10.1038/s41467-023-36226-4.. PMID: 36717557]
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 10002)
  AUTHORS   Dai,H., Zeng,W., Zeng,W., Yan,M., Jiang,P., Li,Y., Zhang,W. and
            Ke,M.
  TITLE     HELZ2 promotes K63-linked polyubiquitination of c-Myc to induce
            retinoblastoma tumorigenesis
  JOURNAL   Med Oncol 39 (1), 11 (2021)
   PUBMED   34761308
  REMARK    GeneRIF: HELZ2 promotes K63-linked polyubiquitination of c-Myc to
            induce retinoblastoma tumorigenesis.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 10002)
  AUTHORS   Katano-Toki,A., Satoh,T., Tomaru,T., Yoshino,S., Ishizuka,T.,
            Ishii,S., Ozawa,A., Shibusawa,N., Tsuchiya,T., Saito,T.,
            Shimizu,H., Hashimoto,K., Okada,S., Yamada,M. and Mori,M.
  TITLE     THRAP3 interacts with HELZ2 and plays a novel role in adipocyte
            differentiation
  JOURNAL   Mol Endocrinol 27 (5), 769-780 (2013)
   PUBMED   23525231
  REMARK    GeneRIF: Thrap3 could play indispensable roles in terminal
            differentiation of adipocytes by enhancing PPARgamma-mediated gene
            activation cooperatively with Helz2.
REFERENCE   5  (bases 1 to 10002)
  AUTHORS   Baltz,A.G., Munschauer,M., Schwanhausser,B., Vasile,A.,
            Murakawa,Y., Schueler,M., Youngs,N., Penfold-Brown,D., Drew,K.,
            Milek,M., Wyler,E., Bonneau,R., Selbach,M., Dieterich,C. and
            Landthaler,M.
  TITLE     The mRNA-bound proteome and its global occupancy profile on
            protein-coding transcripts
  JOURNAL   Mol Cell 46 (5), 674-690 (2012)
   PUBMED   22681889
REFERENCE   6  (bases 1 to 10002)
  AUTHORS   Fairfax,B.P., Makino,S., Radhakrishnan,J., Plant,K., Leslie,S.,
            Dilthey,A., Ellis,P., Langford,C., Vannberg,F.O. and Knight,J.C.
  TITLE     Genetics of gene expression in primary immune cells identifies cell
            type-specific master regulators and roles of HLA alleles
  JOURNAL   Nat Genet 44 (5), 502-510 (2012)
   PUBMED   22446964
  REMARK    GeneRIF: Genetic polymorphisms in PRIC285 gene is associated with
            autoimmune disease.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 10002)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   8  (bases 1 to 10002)
  AUTHORS   Tomaru,T., Satoh,T., Yoshino,S., Ishizuka,T., Hashimoto,K.,
            Monden,T., Yamada,M. and Mori,M.
  TITLE     Isolation and characterization of a transcriptional cofactor and
            its novel isoform that bind the deoxyribonucleic acid-binding
            domain of peroxisome proliferator-activated receptor-gamma
  JOURNAL   Endocrinology 147 (1), 377-388 (2006)
   PUBMED   16239304
REFERENCE   9  (bases 1 to 10002)
  AUTHORS   Surapureddi,S., Yu,S., Bu,H., Hashimoto,T., Yeldandi,A.V.,
            Kashireddy,P., Cherkaoui-Malki,M., Qi,C., Zhu,Y.J., Rao,M.S. and
            Reddy,J.K.
  TITLE     Identification of a transcriptionally active peroxisome
            proliferator-activated receptor alpha -interacting cofactor complex
            in rat liver and characterization of PRIC285 as a coactivator
  JOURNAL   Proc Natl Acad Sci U S A 99 (18), 11836-11841 (2002)
   PUBMED   12189208
REFERENCE   10 (bases 1 to 10002)
  AUTHORS   Deloukas,P., Matthews,L.H., Ashurst,J., Burton,J., Gilbert,J.G.,
            Jones,M., Stavrides,G., Almeida,J.P., Babbage,A.K., Bagguley,C.L.,
            Bailey,J., Barlow,K.F., Bates,K.N., Beard,L.M., Beare,D.M.,
            Beasley,O.P., Bird,C.P., Blakey,S.E., Bridgeman,A.M., Brown,A.J.,
            Buck,D., Burrill,W., Butler,A.P., Carder,C., Carter,N.P.,
            Chapman,J.C., Clamp,M., Clark,G., Clark,L.N., Clark,S.Y.,
            Clee,C.M., Clegg,S., Cobley,V.E., Collier,R.E., Connor,R.,
            Corby,N.R., Coulson,A., Coville,G.J., Deadman,R., Dhami,P.,
            Dunn,M., Ellington,A.G., Frankland,J.A., Fraser,A., French,L.,
            Garner,P., Grafham,D.V., Griffiths,C., Griffiths,M.N., Gwilliam,R.,
            Hall,R.E., Hammond,S., Harley,J.L., Heath,P.D., Ho,S., Holden,J.L.,
            Howden,P.J., Huckle,E., Hunt,A.R., Hunt,S.E., Jekosch,K.,
            Johnson,C.M., Johnson,D., Kay,M.P., Kimberley,A.M., King,A.,
            Knights,A., Laird,G.K., Lawlor,S., Lehvaslaiho,M.H., Leversha,M.,
            Lloyd,C., Lloyd,D.M., Lovell,J.D., Marsh,V.L., Martin,S.L.,
            McConnachie,L.J., McLay,K., McMurray,A.A., Milne,S., Mistry,D.,
            Moore,M.J., Mullikin,J.C., Nickerson,T., Oliver,K., Parker,A.,
            Patel,R., Pearce,T.A., Peck,A.I., Phillimore,B.J.,
            Prathalingam,S.R., Plumb,R.W., Ramsay,H., Rice,C.M., Ross,M.T.,
            Scott,C.E., Sehra,H.K., Shownkeen,R., Sims,S., Skuce,C.D.,
            Smith,M.L., Soderlund,C., Steward,C.A., Sulston,J.E., Swann,M.,
            Sycamore,N., Taylor,R., Tee,L., Thomas,D.W., Thorpe,A., Tracey,A.,
            Tromans,A.C., Vaudin,M., Wall,M., Wallis,J.M., Whitehead,S.L.,
            Whittaker,P., Willey,D.L., Williams,L., Williams,S.A., Wilming,L.,
            Wray,P.W., Hubbard,T., Durbin,R.M., Bentley,D.R., Beck,S. and
            Rogers,J.
  TITLE     The DNA sequence and comparative analysis of human chromosome 20
  JOURNAL   Nature 414 (6866), 865-871 (2001)
   PUBMED   11780052
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL121829.30, AB201715.1 and
            AK074171.1.
            
            On Aug 17, 2007 this sequence version replaced NM_001037335.1.
            
            Summary: The protein encoded by this gene is a nuclear
            transcriptional co-activator for peroxisome proliferator activated
            receptor alpha. The encoded protein contains a zinc finger and is a
            helicase that appears to be part of the peroxisome proliferator
            activated receptor alpha interacting complex. This gene is a member
            of the DNA2/NAM7 helicase gene family. Alternatively spliced
            transcript variants encoding different isoforms have been found for
            this gene. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            CDS exon combination :: AB201715.1 [ECO:0000331]
            RNAseq introns       :: mixed sample support SAMEA1965299,
                                    SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            MANE Ensembl match        :: ENST00000467148.2/ ENSP00000417401.1
            RefSeq Select criteria    :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-56                AL121829.30        103189-103244       c
            57-822              AL121829.30        101461-102226       c
            823-1478            AB201715.1         1-656
            1479-1980           AL121829.30        98153-98654         c
            1981-2622           AL121829.30        97363-98004         c
            2623-3406           AL121829.30        95849-96632         c
            3407-3482           AL121829.30        95383-95458         c
            3483-5980           AL121829.30        92739-95236         c
            5981-9028           AK074171.1         515-3562
            9029-10002          AL121829.30        87091-88064         c
FEATURES             Location/Qualifiers
     source          1..10002
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20q13.33"
     gene            1..10002
                     /gene="HELZ2"
                     /gene_synonym="PDIP-1; PRIC285"
                     /note="helicase with zinc finger 2"
                     /db_xref="GeneID:85441"
                     /db_xref="HGNC:HGNC:30021"
                     /db_xref="MIM:611265"
     exon            1..56
                     /gene="HELZ2"
                     /gene_synonym="PDIP-1; PRIC285"
                     /inference="alignment:Splign:2.1.0"
     exon            57..1170
                     /gene="HELZ2"
                     /gene_synonym="PDIP-1; PRIC285"
                     /inference="alignment:Splign:2.1.0"
     CDS             893..8842
                     /gene="HELZ2"
                     /gene_synonym="PDIP-1; PRIC285"
                     /EC_number="3.1.13.1"
                     /EC_number="3.6.4.13"
                     /note="isoform 1 is encoded by transcript variant 1; PPAR
                     gamma DBD-interacting protein 1; PPARgamma-DNA-binding
                     domain-interacting protein1; helicase with zinc finger 2,
                     transcriptional coactivator; peroxisomal
                     proliferator-activated receptor A-interacting complex 285
                     kDa protein; peroxisomal proliferator-activated receptor
                     alpha-interacting cofactor complex, 285 kD subunit;
                     ATP-dependent RNA helicase PRIC285; PRIC complex; PDIP1;
                     ATP-dependent helicase PRIC285; PPAR-alpha-interacting
                     complex protein 285; PPAR-gamma DNA-binding
                     domain-interacting protein 1; peroxisomal
                     proliferator-activated receptor A interacting complex 285;
                     PPARG-DBD-interacting protein 1; helicase with zinc finger
                     domain 2; 3'-5' exoribonuclease HELZ2"
                     /codon_start=1
                     /product="3'-5' exoribonuclease HELZ2 isoform 1"
                     /protein_id="NP_001032412.2"
                     /db_xref="CCDS:CCDS33508.1"
                     /db_xref="GeneID:85441"
                     /db_xref="HGNC:HGNC:30021"
                     /db_xref="MIM:611265"
                     /translation="MAVWEAEQLGGLQRGDLLTPPAPDGDGRTAPLGQPPGAQLYCPA
                     CLVTCHSQEAFENHCASSEHAQMVAFDQALPWEHRSPPPGLSKFELCPKPDLCEYGDA
                     CTKAHSAQELQEWVRRTQAVELRGQAAWQDGLVPYQERLLAEYQRSSSEVLVLAETLD
                     GVRVTCNQPLMYQAQERKTQYSWTFAVHSEEPLLHVALLKQEPGADFSLVAPGLPPGR
                     LYARGERFRVPSSTADFQVGVRVQAASFGTFEQWVVFDFGRRPVLLQKLGLQLGQGRR
                     PGPCRNLALGHPEEMERWHTGNRHVVPGVERTAEQTALMAKYKGPALALEFNRSSVAS
                     GPISPTNYRQRMHQFLYEEEAAQQQLVAKLTLRGQVFLKTALQTPALNMLFAPPGALY
                     AEVPVPSSLMPDTDQGFLLGRAVSTALVAPVPAPDNTVFEVRLERRASSEQALWLLLP
                     ARCCLALGLQPEARLVLEVQFQIDPMTFRLWHQAVDTLPEEQLVVPDLPTCALPRPWS
                     VPPLRRGNRKQELAVALIAGWGPGDGRRVPPLLIYGPFGTGKTYTLAMASLEVIRRPE
                     TKVLICTHTNSAADIYIREYFHSHVSGGHPEATPLRVMYTDRPLSQTDPVTLQYCCLT
                     DDRQAFRPPTRAELARHRVVVTTTSQARELRVPVGFFSHILIDEAAQMLECEALTPLA
                     YASHGTRLVLAGDHMQVTPRLFSVARARAAEHTLLHRLFLCYQQETHEVARQSRLVFH
                     ENYRCTDAIVSFISRHFYVAKGNPIHARGKVPPHPRHYPLMFCHVAGSPDRDMSMASW
                     LNLAEIAQVVEKVQEAYNTWPSCWGGREQRCICVVSHGAQVSALRQELRRRDLGQVSV
                     GSFEILPGRQFRVVVLSTVHTCQSLLSPGALAPEFFTDARVLNTVLTRAQSQLVVVGD
                     AVALCSFGACGKLWESFIRECVERHSVCPEGLSMEQVEQGVAQRRRWPPRGTQAGAAG
                     NWEAAPEPVGDLAEEQAAVVTAMVKAEPGDEALSPASRDITATTAQTEAAAAPAGDAV
                     KEDVVPGACAAGAAAAAGVESTEAEDAEADFWPWDGELNADDAILRELLDESQKVMVT
                     VGEDGLLDTVARPESLQQARLYENLPPAALRKLLHAEPERYRHCSFVPETFERASAIP
                     LDDASSGPIQVRGRLDCGMAFAGDEVLVQLLSGDKAPEGRLRGRVLGVLKRKRHELAF
                     VCRMDTWDPRIMVPINGSVTKIFVAELKDPSQVPIYSLRKGRLQRVGLERLTAEARHS
                     RLFWVQIVLWRQGFYYPLGIVREVLPEASTWEQGLRILGLEYSLRVPPSDQATITKVL
                     QKYHTELGRVAGRREDCRAFLTFTVDPQGACNLDDALSVRDLGPRCEVAVHITDVASF
                     VPRDGVLDVEARRQGAAFYAPGREPVPMLPASLCQDVLSLLPGRDRLAISLFLTMEKA
                     SGQLKSLRFAPSVVQSDRQLSYEEAEEVIRQHPGAGRELPARLDSVDACVVAACYFSR
                     LLRRHRLRSDCFYEQPDEDGTLGFRAAHIMVKEYMIQFNRLVAEFLVGSECTRTVTPL
                     RWQPAPRSQQLKALCEKHGDRVPLSLHLGHHLHGGGGSPPDTRLHLLASLWKQVQFAA
                     RTQDYEQMVDLVTTDDMHPFLAPAGRDLRKALERSAFGRCARGHQQQGGHYSLQVDWY
                     TWATSPIRRYLDVVLQRQILLALGHGGSAYSARDIDGLCQAFSLQHALAQSYQRRARS
                     LHLAVQLKAQPLDKLGFVVDVEAGSRCFRLLFPSNRETLPDPCPVPYGSLQLAEHPHA
                     LAGRPGLRLLWRRRVYSAQGSSPPLPLPGTVPDPHTLAVETALWKQLLELVELQRWPE
                     AAALIQEKGEASQRRELVQVQRSHCGHFLEVARELGSGDTLQVQLGTSLQHGFLVPSP
                     QLWTVAPGFSLCLEHVERPGDCFSGRVYRAPRDRYRDVDEYACVWEPFCALESATGAV
                     AENDSVTLQHLSVSWEASRTPQGQLQGAFRLEAAFLEENCADINFSCCYLCIRLEGLP
                     APTASPRPGPSSLGPGLNVDPGTYTWVAHGQTQDWDQERRADRQEAPRRVHLFVHHMG
                     MEKVPEEVLRPGTLFTVELLPKQLPDLRKEEAVRGLEEASPLVTSIALGRPVPQPLCR
                     VIPSRFLERQTYNIPGGRHKLNPSQNVAVREALEKPFTVIQGPPGTGKTIVGLHIVFW
                     FHKSNQEQVQPGGPPRGEKRLGGPCILYCGPSNKSVDVLAGLLLRRMELKPLRVYSEQ
                     AEASEFPVPRVGSRKLLRKSPREGRPNQSLRSITLHHRIRQAPNPYSSEIKAFDTRLQ
                     RGELFSREDLVWYKKVLWEARKFELDRHEVILCTCSCAASASLKILDVRQILVDEAGM
                     ATEPETLIPLVQFPQAEKVVLLGDHKQLRPVVKNERLQNLGLDRSLFERYHEDAHMLD
                     TQYRMHEGICAFPSVAFYKSKLKTWQGLRRPPSVLGHAGKESCPVIFGHVQGHERSLL
                     VSTDEGNENSKANLEEVAEVVRITKQLTLGRTVEPQDIAVLTPYNAQASEISKALRRE
                     GIAGVAVSSITKSQGSEWRYVLVSTVRTCAKSDLDQRPTKSWLKKFLGFVVDPNQVNV
                     AVTRAQEGLCLIGDHLLLRCCPLWRSLLDFCEAQQTLVPAGQVRVCRRPTMPS"
     exon            1171..1354
                     /gene="HELZ2"
                     /gene_synonym="PDIP-1; PRIC285"
                     /inference="alignment:Splign:2.1.0"
     exon            1355..1462
                     /gene="HELZ2"
                     /gene_synonym="PDIP-1; PRIC285"
                     /inference="alignment:Splign:2.1.0"
     exon            1463..1980
                     /gene="HELZ2"
                     /gene_synonym="PDIP-1; PRIC285"
                     /inference="alignment:Splign:2.1.0"
     exon            1981..2622
                     /gene="HELZ2"
                     /gene_synonym="PDIP-1; PRIC285"
                     /inference="alignment:Splign:2.1.0"
     exon            2623..3406
                     /gene="HELZ2"
                     /gene_synonym="PDIP-1; PRIC285"
                     /inference="alignment:Splign:2.1.0"
     exon            3407..3482
                     /gene="HELZ2"
                     /gene_synonym="PDIP-1; PRIC285"
                     /inference="alignment:Splign:2.1.0"
     exon            3483..7194
                     /gene="HELZ2"
                     /gene_synonym="PDIP-1; PRIC285"
                     /inference="alignment:Splign:2.1.0"
     exon            7195..7289
                     /gene="HELZ2"
                     /gene_synonym="PDIP-1; PRIC285"
                     /inference="alignment:Splign:2.1.0"
     exon            7290..7421
                     /gene="HELZ2"
                     /gene_synonym="PDIP-1; PRIC285"
                     /inference="alignment:Splign:2.1.0"
     exon            7422..7583
                     /gene="HELZ2"
                     /gene_synonym="PDIP-1; PRIC285"
                     /inference="alignment:Splign:2.1.0"
     exon            7584..7728
                     /gene="HELZ2"
                     /gene_synonym="PDIP-1; PRIC285"
                     /inference="alignment:Splign:2.1.0"
     exon            7729..7845
                     /gene="HELZ2"
                     /gene_synonym="PDIP-1; PRIC285"
                     /inference="alignment:Splign:2.1.0"
     exon            7846..8038
                     /gene="HELZ2"
                     /gene_synonym="PDIP-1; PRIC285"
                     /inference="alignment:Splign:2.1.0"
     exon            8039..8173
                     /gene="HELZ2"
                     /gene_synonym="PDIP-1; PRIC285"
                     /inference="alignment:Splign:2.1.0"
     exon            8174..8392
                     /gene="HELZ2"
                     /gene_synonym="PDIP-1; PRIC285"
                     /inference="alignment:Splign:2.1.0"
     exon            8393..8549
                     /gene="HELZ2"
                     /gene_synonym="PDIP-1; PRIC285"
                     /inference="alignment:Splign:2.1.0"
     exon            8550..8717
                     /gene="HELZ2"
                     /gene_synonym="PDIP-1; PRIC285"
                     /inference="alignment:Splign:2.1.0"
     exon            8718..10002
                     /gene="HELZ2"
                     /gene_synonym="PDIP-1; PRIC285"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9975..9980
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HELZ2"
                     /gene_synonym="PDIP-1; PRIC285"
                     /note="hexamer: GATAAA"
     polyA_site      9981
                     /gene="HELZ2"
                     /gene_synonym="PDIP-1; PRIC285"
                     /note="major polyA site"
     polyA_site      10002
                     /gene="HELZ2"
                     /gene_synonym="PDIP-1; PRIC285"
                     /note="major polyA site"
ORIGIN      
        1 agaatcgaaa ctgagagctc ctgggcaggc tcggcagggc aggcagctcc aggagggctt
       61 cgaaccgtgg ccaacagttc cagtggactg cgtggacccg tgagctcagg agcctcagac
      121 gcctccctgg agagccaagc tggtgttcga ggtggcgcct ccagggtcca ccctgctgcc
      181 caacagcccc gcggccacca gagggccgtc cctggcccgg ctgtgtgccc tggtggacct
      241 gtgtctgggc tgctcccgct gcacccagcg gctcaatgaa agcacctacg tcctccgtag
      301 ggtggagcat gactgctccc gcgagatcct gctggcccgc tttaagcagg ccaccaagag
      361 caaggtctgg cgcgtggtgg gctgccggcc caccttccca aggcccctgt gctaccaagt
      421 ctgccactac tacagccctg ggctcggctg ccggcgccac cgaaaccggt gcacctttgc
      481 ccgcagtcgc gaggaggccc tggtctggac cttcgagcgt cagcacaacc tccagcgcct
      541 atggctgaag gcggaggtgc agggcagcgg ggcccaggga ggggcaggcc gggcggccga
      601 cgccatcctt acggagtttg gcggccgctt cgagctgctt tgctccctct gcttcaggcg
      661 ctgtccccca tgcatctgtc gcgtggaccc ccaggggcag tgccctgagc acggagcctg
      721 cccctccctc ctggcccacg tgagcgccga gggccgccgc aagcaacagt ttgtggtggt
      781 gaggccgcgg ccccgggccg gccagcctcc tgcctactgc aggtttgtgg ggcgtgggca
      841 gccgtgctgg cgtggggagt cccgctgcca gtttgcacac agcgccgtgg agatggctgt
      901 gtgggaggcc gagcagctgg gtggcctcca gcggggggac ctgctcacac cccctgcccc
      961 tgatggcgac gggcgcacgg ccccccttgg ccagccccct ggggcccagc tgtactgccc
     1021 ggcctgcttg gtcacctgcc actctcagga ggccttcgag aaccactgcg catcctcgga
     1081 gcacgcacag atggtggcct tcgaccaggc cctgccctgg gagcaccgtt ccccaccccc
     1141 gggactctcc aagttcgagc tctgcccaaa gcctgacctc tgtgagtatg gggacgcctg
     1201 caccaaggca cactcagcac aggagctgca ggagtgggtc cggcgcacgc aggctgtgga
     1261 gctgcggggg caggcggcct ggcaggacgg gctggtgccc taccaggagc ggctgctggc
     1321 cgagtaccag cgcagcagca gtgaggtcct tgtgctggca gagacccttg atggagtgcg
     1381 tgtcacctgc aaccagcccc tgatgtacca ggcccaggag aggaagaccc agtacagctg
     1441 gacgtttgcc gtccactctg aggagcccct gctacacgtg gccctgctga agcaggagcc
     1501 aggagccgac ttctctctgg tggctcccgg cctcccgcca ggccggctct acgcacgggg
     1561 tgagcgcttc cgtgtgccca gctccactgc cgacttccag gtgggagtgc gtgtgcaggc
     1621 tgcctcgttc ggcacctttg agcaatgggt ggtcttcgac tttggccgcc ggccggtgct
     1681 gctacaaaag ctggggctgc agctgggcca ggggcgtcgc ccaggaccct gcaggaatct
     1741 ggcgctcggc caccctgagg agatggagcg ctggcacact ggcaaccgcc acgtggtgcc
     1801 tggcgtggag cggacggccg agcagacggc cctgatggcc aagtacaagg gccctgccct
     1861 ggccctggag ttcaaccgca gcagcgtggc ctcgggcccc atctcaccaa ccaactatcg
     1921 gcagaggatg caccagtttc tctatgagga ggaggcggct cagcagcagc tggtggccaa
     1981 gctgaccctg cggggccagg tgttcctgaa gacggcattg cagacgccag cgctgaacat
     2041 gctcttcgcg cctccgggag cactgtacgc agaggtcccc gtcccctcct ccctgatgcc
     2101 agacacagac cagggcttcc tgctgggccg ggcggtcagc acagccctgg tggcccctgt
     2161 acctgcaccc gacaatacgg tgttcgaggt gcggctggag aggcgggcca gctcagagca
     2221 ggcgctgtgg ctgctgcttc cggcccgctg ctgcttggcc ctggggctgc agcctgaggc
     2281 ccgcctggtc ctggaggtgc agttccagat tgacccgatg accttccgcc tctggcacca
     2341 ggcagtggac acactgcctg aggagcagct ggtggtgccc gacttgccca cctgcgccct
     2401 gcccagacct tggtctgtcc cacccttgcg gcgtggcaac cgcaagcagg agctggccgt
     2461 ggcgctcatc gcgggctggg gccctgggga tgggaggcgt gtccccccgc tactcatcta
     2521 tggccccttt ggcaccggca agacctacac gctggccatg gcctccctgg aggtcatccg
     2581 gaggcctgaa accaaggtgc tcatctgcac acacaccaac agtgccgccg acatctacat
     2641 ccgggagtat ttccacagcc acgtcagcgg cggccacccc gaggccactc ctctccgtgt
     2701 gatgtacacg gaccggccgc tgagccagac ggacccagtc acgctgcagt actgttgcct
     2761 gaccgacgac cgccaggctt tccgcccgcc cacacgggca gagctggcgc ggcaccgcgt
     2821 ggtggtcacc accacctccc aggcccgtga gctcagggtg ccggtcggct tcttctccca
     2881 cattctcatc gatgaggcgg cccagatgct ggagtgcgag gccctcaccc cgctggccta
     2941 tgcctcgcac ggcacccgcc tcgtgctggc gggcgaccac atgcaggtca caccccggct
     3001 gttcagtgtg gccagggccc gggcggccga gcacacgctg ctgcaccgcc tcttcctgtg
     3061 ctaccagcag gagactcacg aggtggcgcg gcagagccgc ctggtcttcc acgagaacta
     3121 ccgctgcacg gacgccattg tcagcttcat ctcgcggcac ttctacgtgg ccaagggcaa
     3181 ccccatccac gccaggggca aggttccgcc ccacccccgg cactacccgc tcatgttctg
     3241 ccacgtggcg ggcagcccag accgggacat gtccatggcg tcctggctga atctggctga
     3301 gattgcgcag gtcgtcgaga aggtgcagga ggcctacaac acctggccca gctgctgggg
     3361 cggccgcgag cagaggtgca tctgtgtcgt ttcccacggt gcccaggtca gtgcactgag
     3421 gcaggagctg aggaggcggg acctaggcca ggtgtctgtc ggcagttttg agatcctgcc
     3481 agggcggcag ttccgggtcg tggtgctcag cactgtgcac acctgccaga gcctgctcag
     3541 ccctggggca ctggcccctg agttcttcac cgacgcccgc gtgctcaaca ccgtcctgac
     3601 ccgcgcccag tcccagctgg tggtagtggg ggacgccgtg gccctctgct ccttcggggc
     3661 ctgcggcaag ctctgggaga gcttcatccg tgagtgcgtg gagcggcaca gtgtctgccc
     3721 cgagggcctg tccatggagc aggtcgagca gggtgtggcg cagagacggc gctggcctcc
     3781 ccgaggcaca caggctgggg cagcggggaa ctgggaggct gccccagagc cagtagggga
     3841 cctggccgag gagcaggcgg ctgtggtgac ggccatggtg aaggcagagc cgggagatga
     3901 ggctctgagc ccagcatccc gtgacatcac ggcaaccaca gcgcagacgg aggctgcggc
     3961 agcaccagca ggagacgcag tgaaggaaga cgtggtgccc ggggcctgtg cggcaggagc
     4021 ggctgctgca gcgggcgtgg agtccacgga ggctgaggat gcagaggctg acttctggcc
     4081 gtgggacggg gagctcaacg ctgacgacgc catcctacgg gagcttctgg acgagagcca
     4141 gaaggtgatg gtgaccgtcg gggaggacgg gctgctggac actgtcgcca ggcccgagtc
     4201 cctgcagcag gcccggctgt acgagaacct gcccccggct gcgctacgga agctgctgca
     4261 cgcggagccc gagcggtacc gccactgctc tttcgtgcca gagaccttcg agcgggcgtc
     4321 agccatcccg ctggacgatg cctcctcggg ccccatccag gtcaggggcc gcctggactg
     4381 tgggatggcc ttcgccgggg atgaggtgct ggtgcagctc ctttcgggag acaaggcgcc
     4441 cgaggggcgg cttcggggcc gcgtgctggg cgtgctgaag aggaagaggc acgagctggc
     4501 gtttgtgtgc cgcatggaca cgtgggaccc gcgcatcatg gtccccatca atggctccgt
     4561 gaccaagatc ttcgtggccg agctgaagga cccatcgcag gtccccatct acagcctccg
     4621 gaagggccgg ctgcagcgtg tggggcttga gaggctcacc gccgaggccc ggcacagccg
     4681 gctcttctgg gtccaaattg tcctgtggcg gcaaggcttc tactacccgc tgggcatcgt
     4741 ccgggaggtg ctgcctgagg ccagcacctg ggagcagggc ctccgcatcc tcggcctgga
     4801 gtacagcttg agggtgcccc cgtcggacca ggccaccatc accaaggtgc tgcagaaata
     4861 ccacacggag cttggccggg ttgccggccg ccgagaggac tgccgcgcct tcttgacctt
     4921 cactgtggac ccccagggcg cctgcaacct cgatgatgcc ctcagtgtcc gagacctggg
     4981 tcccaggtgc gaggtggctg tgcacatcac tgatgtggcc agcttcgtgc ccagggacgg
     5041 ggtgctggac gtggaggcgc gaaggcaggg cgctgcgttc tatgcccccg gcagggagcc
     5101 agtgcccatg ctgccggcca gcctctgcca ggacgtcctc agcctcctgc ctggccggga
     5161 ccgcctggcc atctccctgt tcctcaccat ggagaaggcc agtggccagc tgaagagcct
     5221 gcgctttgca ccctccgtgg tccagtctga ccgccagctg tcctacgagg aggcggagga
     5281 ggtgatcagg cagcacccgg gtgccggccg tgagctgccg gcccgcctgg actccgtgga
     5341 cgcctgcgtc gtggccgcgt gctacttctc tcggctgctg cgccggcacc gcctgcggtc
     5401 cgactgcttc tatgagcagc cggacgagga cggcaccctg ggcttccgcg cggcccacat
     5461 catggtgaag gagtacatga ttcagtttaa taggctcgtg gctgagttcc tggtgggcag
     5521 cgagtgcacg cggacggtca cgcctctgcg gtggcagcca gcaccccgca gccagcagct
     5581 caaggccctg tgtgagaagc atggggaccg ggtgcccctg tcactgcacc tcggccacca
     5641 cctgcacggc ggcgggggca gtccccccga cacgcggctg cacctcctgg cctccctctg
     5701 gaagcaggtc cagtttgctg cccgcaccca ggactacgaa cagatggtgg acttggtcac
     5761 cacggacgac atgcacccat tcctggctcc tgcaggccgc gacctccgca aggccctgga
     5821 gcgctcggcg ttcggccgct gcgcccgggg ccaccagcag cagggcggcc actactcgct
     5881 gcaggtggac tggtacacgt gggccacctc gcccatccgc aggtacctgg acgtggtgtt
     5941 gcagcggcag atcctgctgg cgctgggcca tgggggctct gcctactctg ccagggacat
     6001 cgatgggctc tgccaggcct tcagcctcca gcacgcactt gcccagagct atcagcggcg
     6061 ggcgcgcagc ctgcacctgg ccgtgcagct caaggcccag cctctggaca agctgggctt
     6121 cgtggtggac gtggaggcgg gctcccgctg cttccggctg ctcttcccca gcaaccggga
     6181 gacgctgcct gacccctgcc ccgtccccta cggctccctg cagctggccg agcaccccca
     6241 cgccctggca ggccggccgg gcctgcggct cctgtggcgg cgccgtgtct actcagcgca
     6301 gggatccagc ccgcccctgc cactgcctgg cactgtgccg gacccacaca ccctggccgt
     6361 ggagacggcc ctgtggaagc agctgctgga gctggtggag ctgcagcgct ggccggaggc
     6421 ggctgctctc atccaggaga agggcgaggc gtcccagcgg cgggagctgg tgcaggtgca
     6481 gcggagccac tgtggccatt tcctggaggt ggcccgggag ctgggcagtg gggacaccct
     6541 gcaggtgcag ctcggcacca gcctgcagca cggcttcctg gtaccgagcc ctcagctctg
     6601 gacggtggca ccgggcttca gcctctgcct ggagcacgtg gagcggcccg gagactgctt
     6661 ctcaggccgt gtgtaccggg ccccgaggga ccggtaccgc gacgtggatg agtacgcctg
     6721 cgtgtgggaa ccattctgcg ccctggagtc ggccaccggc gcggttgccg agaatgactc
     6781 cgtcacactt cagcacctga gtgtctcctg ggaggcgtca cggacgccgc aggggcagct
     6841 gcagggcgcc ttccgcctgg aggccgcctt cctcgaggag aactgtgccg acatcaactt
     6901 cagctgctgc tacctctgca tccggctcga ggggctgccg gctcccacgg ccagcccacg
     6961 ccctgggccc agcagcctcg gccctggcct gaatgttgac cccggcacgt atacctgggt
     7021 ggcccacggg cagacgcagg actgggacca ggagcgccgg gcagaccggc aggaggctcc
     7081 cagacgggtg cacctcttcg tccaccacat gggcatggag aaggttccgg aagaggtgct
     7141 gaggccgggc accctgttca ccgttgagct gctgcccaag cagcttcctg atctccgcaa
     7201 ggaggaagcc gtgcgtggac tagaggaggc gtccccgctg gtcaccagca tcgcactggg
     7261 ccggcctgtc ccgcagcccc tctgcagagt gatccccagc aggttcctgg agcggcagac
     7321 ctacaacatc cccggaggcc gccacaagct gaaccccagc cagaacgtgg cggtcaggga
     7381 ggctctggag aagcctttca cggtcattca gggcccacca ggtacaggga agacgatcgt
     7441 gggcctccac atcgtattct ggtttcataa atcaaaccag gagcaggtgc agcccggagg
     7501 ccccccccgt ggggagaagc ggctgggggg tccctgcatc ttgtactgcg gcccctccaa
     7561 caagtcggtg gatgtcctgg caggactgct cctgagaagg atggagctga agcccctccg
     7621 tgtgtacagt gagcaggctg aggccagcga gttcccagtg ccgcgtgtgg gcagcaggaa
     7681 gctgctcagg aagagccccc gggaggggag gccgaaccag agcctcagga gcatcaccct
     7741 gcaccaccgg atccggcagg cccccaaccc ttactcgtcg gaaatcaagg cctttgacac
     7801 ccggctgcag agaggggagc tcttctccag ggaggacctg gtctggtaca agaaggtctt
     7861 gtgggaggct cggaagttcg agctggaccg gcatgaggtc atcctctgca cctgctcctg
     7921 tgcagcctct gccagcctca aaatcctgga cgtgaggcag atccttgttg acgaggcagg
     7981 catggccacg gaacctgaaa ccctcatccc cctggtgcag ttcccacagg ccgagaaggt
     8041 ggttcttctc ggagaccaca agcagctgcg gcctgtggtc aagaatgagc ggctgcaaaa
     8101 cctgggtctg gaccggtctc tgttcgagcg gtaccacgag gacgcacata tgctggacac
     8161 tcagtaccgc atgcatgagg gcatctgtgc cttcccctct gtggcgttct acaagagcaa
     8221 gctgaagacg tggcagggcc tgaggaggcc gcccagtgtc ctgggccacg ctggcaagga
     8281 gagctgccct gtcatctttg gccacgtgca gggccacgag cggagcctgc tggtgtccac
     8341 ggacgaaggg aatgagaact ccaaggccaa cctggaggag gtggctgagg tggtccgtat
     8401 caccaagcag ctgaccctgg ggaggaccgt agagccccag gacatcgccg tcctcacgcc
     8461 ctacaacgcg caggcctctg agatcagcaa ggcccttcgg cgagagggca tcgccggggt
     8521 ggccgtgtcc tccatcacca agagccaggg gagcgagtgg cgctatgtgc tggtgagcac
     8581 cgtccgcacc tgtgccaaga gcgacctgga ccagcggccc accaagagct ggctcaagaa
     8641 gtttctgggc ttcgttgtgg accccaacca agtgaatgtg gctgtcacgc gggcccagga
     8701 ggggctctgc ctgatcggag accacctcct tctgcgctgc tgccccctct ggcgtagcct
     8761 cctggacttc tgcgaggctc agcagaccct cgtgcctgcc ggccaggtgc gcgtctgcag
     8821 gaggccaact atgccttcct gaagagccct ctccacctgc aaggtgccag gactgggagg
     8881 gaaagtccag ggccccccca ccctccccac caccctccgc catcagcctg cctgggccag
     8941 cctgcctcct ggcctcagga tctccctggg gcaggaagtg ggtggacttg gaggaggaac
     9001 ggcaagggcc ggtccccaca ctcttgctcc tcctgggcat ctggggacag gaacacctct
     9061 cagggtggcc aagaggggct tccttgggac cagtgcaagg aagttctgct gtccctggga
     9121 agtctcagaa ccaggaagga aaccctgtgg aggctgccct ttgacctttg gacgccacct
     9181 gtaatcactc tggatggtgt ctgtggagtt caggtttatt ttctatttaa atgaactaaa
     9241 agccagcatc ctgccgggcg tggtggctca cgcccataat ccagcacttt gggaagccga
     9301 ggcgggcaga tcacctgagg tcaggagttc gagaccatcc tggccaacat ggtgaaaccc
     9361 cgtctccact aaaaatacaa aattagctgg gcgtggtggc gggcgcctgt aatcccagct
     9421 acttgggagg ctgaggcagg agaatcgctt gactccagga ggcgcaggct gcagtgagct
     9481 ctgatggtac cactgcactc cagcctgggt gacagagcga gaccctgtct taaaaaaaaa
     9541 aacaaaacaa aaccagcatc tgttactgga acagagacct cagcccaagc tcaggacaag
     9601 gaggcctccc tgggagaggg ggcctttctc ccaccatcac cgcctgtgtc cttcaggggc
     9661 tgctggaggg cagcccaaag ccaggggccc accccgcccc accccacccc accccaccct
     9721 ctgaaatgtg aaaagcctgc actcttcctg tgggctgcgc agtgggctcg ggggttgggg
     9781 ggtgccgggg cgatgcttcc accctggact gagtgttcgg ggcagcctgc tcggctgtat
     9841 gtttctcgca cgctcacccg cttcagtgcg gctctgtccc tgctgccgcc tccagtcctg
     9901 agggaggggc ttgccccacc ttctacattc caatttttat atctttgaat tatgtgatta
     9961 gatattaatt taatgataaa accactctga aagctcttct ca
//
