LOCUS       NM_014877              13817 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens helicase with zinc finger (HELZ), transcript variant
            1, mRNA.
ACCESSION   NM_014877 XM_375485
VERSION     NM_014877.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 13817)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 13817)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 13817)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   4  (bases 1 to 13817)
  AUTHORS   Maio,N., Singh,A., Uhrigshardt,H., Saxena,N., Tong,W.H. and
            Rouault,T.A.
  TITLE     Cochaperone binding to LYR motifs confers specificity of iron
            sulfur cluster delivery
  JOURNAL   Cell Metab 19 (3), 445-457 (2014)
   PUBMED   24606901
REFERENCE   5  (bases 1 to 13817)
  AUTHORS   Baltz,A.G., Munschauer,M., Schwanhausser,B., Vasile,A.,
            Murakawa,Y., Schueler,M., Youngs,N., Penfold-Brown,D., Drew,K.,
            Milek,M., Wyler,E., Bonneau,R., Selbach,M., Dieterich,C. and
            Landthaler,M.
  TITLE     The mRNA-bound proteome and its global occupancy profile on
            protein-coding transcripts
  JOURNAL   Mol Cell 46 (5), 674-690 (2012)
   PUBMED   22681889
REFERENCE   6  (bases 1 to 13817)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   7  (bases 1 to 13817)
  AUTHORS   Beausoleil,S.A., Jedrychowski,M., Schwartz,D., Elias,J.E.,
            Villen,J., Li,J., Cohn,M.A., Cantley,L.C. and Gygi,S.P.
  TITLE     Large-scale characterization of HeLa cell nuclear phosphoproteins
  JOURNAL   Proc Natl Acad Sci U S A 101 (33), 12130-12135 (2004)
   PUBMED   15302935
REFERENCE   8  (bases 1 to 13817)
  AUTHORS   Hamamoto,R., Furukawa,Y., Morita,M., Iimura,Y., Silva,F.P., Li,M.,
            Yagyu,R. and Nakamura,Y.
  TITLE     SMYD3 encodes a histone methyltransferase involved in the
            proliferation of cancer cells
  JOURNAL   Nat Cell Biol 6 (8), 731-740 (2004)
   PUBMED   15235609
REFERENCE   9  (bases 1 to 13817)
  AUTHORS   Nagai,H., Yabe,A., Mine,N., Mikami,I., Fujiwara,H., Terada,Y.,
            Hirano,A., Tsuneizumi,M., Yokota,T. and Emi,M.
  TITLE     Down-regulation in human cancers of DRHC, a novel helicase-like
            gene from 17q25.1 that inhibits cell growth
  JOURNAL   Cancer Lett 193 (1), 41-47 (2003)
   PUBMED   12691822
  REMARK    GeneRIF: Results suggest that loss of expression of DRHC may play a
            role in human carcinogenesis.
REFERENCE   10 (bases 1 to 13817)
  AUTHORS   Wagner,D.S., Gan,L. and Klein,W.H.
  TITLE     Identification of a differentially expressed RNA helicase by gene
            trapping
  JOURNAL   Biochem Biophys Res Commun 262 (3), 677-684 (1999)
   PUBMED   10471385
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC007448.14 and AC005544.1.
            
            On Nov 23, 2018 this sequence version replaced NM_014877.3.
            
            Summary: HELZ is a member of the superfamily I class of RNA
            helicases. RNA helicases alter the conformation of RNA by unwinding
            double-stranded regions, thereby altering the biologic activity of
            the RNA molecule and regulating access to other proteins (Wagner et
            al., 1999 [PubMed 10471385]).[supplied by OMIM, Mar 2008].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.239143.1,
                                           SRR1660807.108853.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000358691.10/ ENSP00000351524.5
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-49                AC007448.14        88269-88317
            50-105              AC007448.14        89626-89681
            106-162             AC007448.14        93976-94032
            163-390             AC007448.14        114643-114870
            391-427             AC007448.14        117530-117566
            428-552             AC007448.14        130022-130146
            553-609             AC007448.14        132280-132336
            610-661             AC007448.14        137995-138046
            662-737             AC007448.14        139423-139498
            738-936             AC007448.14        143110-143308
            937-1044            AC007448.14        143769-143876
            1045-1342           AC007448.14        144849-145146
            1343-1610           AC007448.14        154539-154806
            1611-1944           AC007448.14        165669-166002
            1945-2075           AC007448.14        166857-166987
            2076-2255           AC007448.14        172389-172568
            2256-2357           AC007448.14        173103-173204
            2358-2536           AC007448.14        182241-182419
            2537-2655           AC007448.14        183480-183598
            2656-2801           AC007448.14        184751-184896
            2802-2949           AC007448.14        187575-187722
            2950-3133           AC005544.1         3535-3718
            3134-3362           AC005544.1         5451-5679
            3363-3567           AC005544.1         12794-12998
            3568-3619           AC005544.1         17635-17686
            3620-3810           AC005544.1         18489-18679
            3811-4018           AC005544.1         21037-21244
            4019-4098           AC005544.1         27246-27325
            4099-4669           AC005544.1         31963-32533
            4670-4904           AC005544.1         32923-33157
            4905-5421           AC005544.1         33964-34480
            5422-5674           AC005544.1         54568-54820
            5675-13817          AC005544.1         63063-71205
FEATURES             Location/Qualifiers
     source          1..13817
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q24.2"
     gene            1..13817
                     /gene="HELZ"
                     /gene_synonym="DHRC; DRHC; HUMORF5"
                     /note="helicase with zinc finger"
                     /db_xref="GeneID:9931"
                     /db_xref="HGNC:HGNC:16878"
                     /db_xref="MIM:606699"
     exon            1..49
                     /gene="HELZ"
                     /gene_synonym="DHRC; DRHC; HUMORF5"
                     /inference="alignment:Splign:2.1.0"
     exon            50..105
                     /gene="HELZ"
                     /gene_synonym="DHRC; DRHC; HUMORF5"
                     /inference="alignment:Splign:2.1.0"
     exon            106..162
                     /gene="HELZ"
                     /gene_synonym="DHRC; DRHC; HUMORF5"
                     /inference="alignment:Splign:2.1.0"
     exon            163..390
                     /gene="HELZ"
                     /gene_synonym="DHRC; DRHC; HUMORF5"
                     /inference="alignment:Splign:2.1.0"
     CDS             181..6009
                     /gene="HELZ"
                     /gene_synonym="DHRC; DRHC; HUMORF5"
                     /note="isoform 1 is encoded by transcript variant 1;
                     helicase with zinc finger domain; probable helicase with
                     zinc finger domain; down-regulated in human cancers
                     protein"
                     /codon_start=1
                     /product="probable helicase with zinc finger domain
                     isoform 1"
                     /protein_id="NP_055692.3"
                     /db_xref="CCDS:CCDS42374.1"
                     /db_xref="GeneID:9931"
                     /db_xref="HGNC:HGNC:16878"
                     /db_xref="MIM:606699"
                     /translation="MEDRRAEKSCEQACESLKRQDYEMALKHCTEALLSLGQYSMADF
                     TGPCPLEIERIKIESLLYRIASFLQLKNYVQADEDCRHVLGEGLAKGEDAFRAVLCCM
                     QLKGKLQPVSTILAKSLTGESLNGMVTKDLTRLKTLLSETETATSNALSGYHVEDLDE
                     GSCNGWHFRPPPRGITSSEEYTLCKRFLEQGICRYGAQCTSAHSQEELAEWQKRYASR
                     LIKLKQQNENKQLSGSYMETLIEKWMNSLSPEKVLSECIEGVKVEHNPDLSVTVSTKK
                     SHQTWTFALTCKPARMLYRVALLYDAHRPHFSIIAISAGDSTTQVSQEVPENCQEWIG
                     GKMAQNGLDHYVYKVGIAFNTEIFGTFRQTIVFDFGLEPVLMQRVMIDAASTEDLEYL
                     MHAKQQLVTTAKRWDSSSKTIIDFEPNETTDLEKSLLIRYQIPLSADQLFTQSVLDKS
                     LTKSNYQSRLHDLLYIEEIAQYKEISKFNLKVQLQILASFMLTGVSGGAKYAQNGQLF
                     GRFKLTETLSEDTLAGRLVMTKVNAVYLLPVPKQKLVQTQGTKEKVYEATIEEKTKEY
                     IFLRLSRECCEELNLRPDCDTQVELQFQLNRLPLCEMHYALDRIKDNGVLFPDISMTP
                     TIPWSPNRQWDEQLDPRLNAKQKEAVLAITTPLAIQLPPVLIIGPYGTGKTFTLAQAV
                     KHILQQQETRILICTHSNSAADLYIKDYLHPYVEAGNPQARPLRVYFRNRWVKTVHPV
                     VHQYCLISSAHSTFQMPQKEDILKHRVVVVTLNTSQYLCQLDLEPGFFTHILLDEAAQ
                     AMECETIMPLALATQNTRIVLAGDHMQLSPFVYSEFARERNLHVSLLDRLYEHYPAEF
                     PCRILLCENYRSHEAIINYTSELFYEGKLMASGKQPAHKDFYPLTFFTARGEDVQEKN
                     STAFYNNAEVFEVVERVEELRRKWPVAWGKLDDGSIGVVTPYADQVFRIRAELRKKRL
                     SDVNVERVLNVQGKQFRVLFLSTVRTRHTCKHKQTPIKKKEQLLEDSTEDLDYGFLSN
                     YKLLNTAITRAQSLVAVVGDPIALCSIGRCRKFWERFIALCHENSSLHGITFEQIKAQ
                     LEALELKKTYVLNPLAPEFIPRALRLQHSGSTNKQQQSPPKGKSLHHTQNDHFQNDGI
                     VQPNPSVLIGNPIRAYTPPPPLGPHPNLGKSPSPVQRIDPHTGTSILYVPAVYGGNVV
                     MSVPLPVPWTGYQGRFAVDPRIITHQAAMAYNMNLLQTHGRGSPIPYGLGHHPPVTIG
                     QPQNQHQEKDQHEQNRNGKSDTNNSGPEINKIRTPEKKPTEPKQVDLESNPQNRSPES
                     RPSVVYPSTKFPRKDNLNPRHINLPLPAPHAQYAIPNRHFHPLPQLPRPPFPIPQQHT
                     LLNQQQNNLPEQPNQIPPQPNQVVQQQSQLNQQPQQPPPQLSPAYQAGPNNAFFNSAV
                     AHRPQSPPAEAVIPEQQPPPMLQEGHSPLRAIAQPGPILPSHLNSFIDENPSGLPIGE
                     ALDRIHGSVALETLRQQQARFQQWSEHHAFLSQGSAPYPHHHHPHLQHLPQPPLGLHQ
                     PPVRADWKLTSSAEDEVETTYSRFQDLIRELSHRDQSETRELAEMPPPQSRLLQYRQV
                     QSRSPPAVPSPPSSTDHSSHFSNFNDNSRDIEVASNPAFPQRLPPQIFNSPFSLPSEH
                     LAPPPLKYLAPDGAWTFANLQQNHLMGPGFPYGLPPLPHRPPQNPFVQIQNHQHAIGQ
                     EPFHPLSSRTVSSSSLPSLEEYEPRGPGRPLYQRRISSSSVQPCSEEVSTPQDSLAQC
                     KELQDHSNQSSFNFSSPESWVNTTSSTPYQNIPCNGSSRTAQPRELIAPPKTVKPPED
                     QLKSENLEVSSSFNYSVLQHLGQFPPLMPNKQIAESANSSSPQSSAGGKPAMSYASAL
                     RAPPKPRPPPEQAKKSSDPLSLFQELSLGSSSGSNGFYSYFK"
     exon            391..427
                     /gene="HELZ"
                     /gene_synonym="DHRC; DRHC; HUMORF5"
                     /inference="alignment:Splign:2.1.0"
     exon            428..552
                     /gene="HELZ"
                     /gene_synonym="DHRC; DRHC; HUMORF5"
                     /inference="alignment:Splign:2.1.0"
     exon            553..609
                     /gene="HELZ"
                     /gene_synonym="DHRC; DRHC; HUMORF5"
                     /inference="alignment:Splign:2.1.0"
     exon            610..661
                     /gene="HELZ"
                     /gene_synonym="DHRC; DRHC; HUMORF5"
                     /inference="alignment:Splign:2.1.0"
     exon            662..737
                     /gene="HELZ"
                     /gene_synonym="DHRC; DRHC; HUMORF5"
                     /inference="alignment:Splign:2.1.0"
     exon            738..936
                     /gene="HELZ"
                     /gene_synonym="DHRC; DRHC; HUMORF5"
                     /inference="alignment:Splign:2.1.0"
     exon            937..1044
                     /gene="HELZ"
                     /gene_synonym="DHRC; DRHC; HUMORF5"
                     /inference="alignment:Splign:2.1.0"
     exon            1045..1342
                     /gene="HELZ"
                     /gene_synonym="DHRC; DRHC; HUMORF5"
                     /inference="alignment:Splign:2.1.0"
     exon            1343..1610
                     /gene="HELZ"
                     /gene_synonym="DHRC; DRHC; HUMORF5"
                     /inference="alignment:Splign:2.1.0"
     exon            1611..1944
                     /gene="HELZ"
                     /gene_synonym="DHRC; DRHC; HUMORF5"
                     /inference="alignment:Splign:2.1.0"
     exon            1945..2075
                     /gene="HELZ"
                     /gene_synonym="DHRC; DRHC; HUMORF5"
                     /inference="alignment:Splign:2.1.0"
     exon            2076..2255
                     /gene="HELZ"
                     /gene_synonym="DHRC; DRHC; HUMORF5"
                     /inference="alignment:Splign:2.1.0"
     exon            2256..2357
                     /gene="HELZ"
                     /gene_synonym="DHRC; DRHC; HUMORF5"
                     /inference="alignment:Splign:2.1.0"
     exon            2358..2536
                     /gene="HELZ"
                     /gene_synonym="DHRC; DRHC; HUMORF5"
                     /inference="alignment:Splign:2.1.0"
     exon            2537..2655
                     /gene="HELZ"
                     /gene_synonym="DHRC; DRHC; HUMORF5"
                     /inference="alignment:Splign:2.1.0"
     exon            2656..2801
                     /gene="HELZ"
                     /gene_synonym="DHRC; DRHC; HUMORF5"
                     /inference="alignment:Splign:2.1.0"
     exon            2802..2949
                     /gene="HELZ"
                     /gene_synonym="DHRC; DRHC; HUMORF5"
                     /inference="alignment:Splign:2.1.0"
     exon            2950..3133
                     /gene="HELZ"
                     /gene_synonym="DHRC; DRHC; HUMORF5"
                     /inference="alignment:Splign:2.1.0"
     exon            3134..3362
                     /gene="HELZ"
                     /gene_synonym="DHRC; DRHC; HUMORF5"
                     /inference="alignment:Splign:2.1.0"
     exon            3363..3567
                     /gene="HELZ"
                     /gene_synonym="DHRC; DRHC; HUMORF5"
                     /inference="alignment:Splign:2.1.0"
     exon            3568..3619
                     /gene="HELZ"
                     /gene_synonym="DHRC; DRHC; HUMORF5"
                     /inference="alignment:Splign:2.1.0"
     exon            3620..3810
                     /gene="HELZ"
                     /gene_synonym="DHRC; DRHC; HUMORF5"
                     /inference="alignment:Splign:2.1.0"
     exon            3811..4018
                     /gene="HELZ"
                     /gene_synonym="DHRC; DRHC; HUMORF5"
                     /inference="alignment:Splign:2.1.0"
     exon            4019..4098
                     /gene="HELZ"
                     /gene_synonym="DHRC; DRHC; HUMORF5"
                     /inference="alignment:Splign:2.1.0"
     exon            4099..4669
                     /gene="HELZ"
                     /gene_synonym="DHRC; DRHC; HUMORF5"
                     /inference="alignment:Splign:2.1.0"
     exon            4670..4904
                     /gene="HELZ"
                     /gene_synonym="DHRC; DRHC; HUMORF5"
                     /inference="alignment:Splign:2.1.0"
     exon            4905..5421
                     /gene="HELZ"
                     /gene_synonym="DHRC; DRHC; HUMORF5"
                     /inference="alignment:Splign:2.1.0"
     exon            5422..5674
                     /gene="HELZ"
                     /gene_synonym="DHRC; DRHC; HUMORF5"
                     /inference="alignment:Splign:2.1.0"
     exon            5675..13817
                     /gene="HELZ"
                     /gene_synonym="DHRC; DRHC; HUMORF5"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7014..7019
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HELZ"
                     /gene_synonym="DHRC; DRHC; HUMORF5"
                     /note="hexamer: ATTATA"
     polyA_site      7033
                     /gene="HELZ"
                     /gene_synonym="DHRC; DRHC; HUMORF5"
     regulatory      13797..13802
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HELZ"
                     /gene_synonym="DHRC; DRHC; HUMORF5"
                     /note="hexamer: AATAAA"
     polyA_site      13817
                     /gene="HELZ"
                     /gene_synonym="DHRC; DRHC; HUMORF5"
                     /note="major polyA site"
ORIGIN      
        1 gtggggacgt tgacaagtgg atccaagatg gcgtagaaag taatgacagg atttggatga
       61 agtaatggat cactaatgaa ctacagtgct ggtgagatgt gtaagaaatt ataaagagct
      121 ctgatgggct atttgggtga tacccagtgc agtgaactgc aggatttttg tccctgagtc
      181 atggaagaca gaagagctga aaagtcatgt gaacaagcat gtgaatcact taagaggcag
      241 gactatgaaa tggccctcaa gcactgcaca gaggcccttc tttctcttgg ccagtactcc
      301 atggcagact tcacagggcc ttgtccattg gaaatagaac gcatcaaaat cgagagtctt
      361 ctctacagaa ttgcctcatt tttgcaactg aaaaattatg tgcaagctga tgaagattgt
      421 agacatgtgc tgggagaagg actggccaag ggagaagatg cctttcgggc agtgctttgc
      481 tgcatgcagc tgaaagggaa gctccaacct gtatccacca ttcttgccaa gtcactcaca
      541 ggagagtccc tgaatgggat ggtaacaaag gatttgacaa gactaaaaac acttctctca
      601 gaaacagaga cagcaactag taacgccctc tctggatatc acgtggaaga cttagatgag
      661 gggtcttgta atggttggca tttccgccca ccacctaggg gaatcacaag cagcgaggaa
      721 tatactttgt gtaaaagatt tttagaacaa ggaatctgta ggtatggtgc ccagtgtact
      781 tcagcacatt cccaggaaga actagcagaa tggcagaaaa gatatgcttc acggctgata
      841 aaattgaaac agcaaaatga gaataaacag ctctcaggca gttacatgga aaccttgata
      901 gaaaagtgga tgaattcatt gtctcctgag aaagtgctta gtgaatgtat agaaggagta
      961 aaggtagagc acaatcctga cctgtcagtt actgtcagca ccaaaaaatc ccaccagaca
     1021 tggacctttg ctctcacttg taagcctgca agaatgctgt atcgtgtagc attgctttat
     1081 gatgctcatc gtcctcattt tagtatcatt gcaatatctg ccggagatag tactacccag
     1141 gtatcacaag aagtcccaga aaactgtcaa gaatggatag gaggaaagat ggcccaaaat
     1201 ggattagatc attacgtgta taaagtcggg atagcattta acacagaaat atttggaact
     1261 tttcgccaaa ccatagtttt cgactttgga ttggaaccag tactcatgca aagagtaatg
     1321 attgatgcag cttctacaga agatctcgaa tacctgatgc atgcaaaaca gcagctagta
     1381 accacagcta aacgttggga ttcttcctct aagactatta tagattttga acctaatgaa
     1441 actactgatt tggagaagag ccttcttatc agataccaaa ttcccctctc tgctgaccag
     1501 ctatttactc agtccgtttt agacaaatca ttgaccaaga gcaactatca gtcacggtta
     1561 catgaccttc tttatattga ggagatagcc cagtataaag aaatcagcaa gttcaacctt
     1621 aaagtgcaat tgcagattct ggcaagcttc atgctcactg gtgtttctgg aggtgcaaag
     1681 tatgctcaga atggacaact ttttggtcgc tttaagctta ctgaaacact ttctgaagat
     1741 actttggctg gacgactggt gatgaccaaa gtcaatgctg tttatttatt accagtccct
     1801 aaacagaagt tagtacagac ccagggaacc aaagagaagg tttatgaagc tactattgaa
     1861 gaaaaaacaa aggaatatat atttttaagg ctatctaggg aatgctgtga agaacttaat
     1921 cttcggcctg actgtgacac acaggttgaa cttcagtttc aattaaatcg attacccctc
     1981 tgtgaaatgc actatgcact agacaggatc aaggacaatg gggttttgtt tccagacatc
     2041 agtatgactc ccaccatacc atggagtcct aacagacaat gggatgaaca gttggatcct
     2101 cgactaaatg caaaacagaa agaggctgtt ctggccatta ccactccact tgcaatccag
     2161 ctgccgcctg tgcttatcat cggaccctat gggacaggca aaacgttcac tctagctcag
     2221 gctgtcaaac atattctgca gcaacaggag actaggattc tcatttgcac ccattctaat
     2281 agtgctgctg atctctacat aaaggattat ttacatccat atgtagaagc aggcaatccc
     2341 caggcaagac ctctcagggt atatttcaga aatcgctggg taaagactgt ccacccagtt
     2401 gtgcatcagt actgtttgat ctcaagcgca cattccacct ttcagatgcc ccagaaagaa
     2461 gatattctta aacatcgagt ggtggttgtt accttgaata cttcccagta cctctgtcag
     2521 ttggaccttg aacctgggtt ttttacacac attctattag atgaagctgc ccaggccatg
     2581 gagtgtgaaa ccattatgcc tctagcatta gcaactcaaa acactcggat tgtcttggct
     2641 ggtgatcaca tgcagctcag tccttttgtt tacagcgagt ttgccaggga gagaaacctt
     2701 cacgtttcat tacttgaccg actctatgag cattaccctg ctgagttccc atgtaggatt
     2761 ctcctgtgtg agaactaccg ctcccatgaa gctatcatca attatacctc tgagcttttc
     2821 tatgagggca aactgatggc cagtgggaag cagccagcac acaaagattt ctacccacta
     2881 actttcttta cagcacgagg agaagatgta caagaaaaaa atagcacagc tttttataat
     2941 aatgcagagg tgtttgaagt ggtggaacgt gtagaagagt taagaaggaa gtggccagta
     3001 gcgtggggga agttagatga tggcagtatt ggtgtggtga ctccatatgc tgatcaagtg
     3061 tttagaatac gtgctgaact tcgaaaaaag agattatctg atgttaatgt agaaagggtg
     3121 ctaaatgttc aaggaaagca attcagagtt ttgtttctta gcacagtacg tacaagacat
     3181 acttgtaaac ataaacagac accaattaaa aagaaagagc aacttctgga agattccaca
     3241 gaggacttag attatggttt tttatctaac tacaagcttc tcaatactgc catcacaaga
     3301 gcacaatccc tggttgctgt ggtgggtgat cccattgctc tgtgctctat tggaagatgc
     3361 aggaaatttt gggaacggtt tattgccctg tgtcatgaaa acagtagcct acatggaatc
     3421 acttttgaac agatcaaagc ccagttagag gctttagaac taaagaagac atatgtgttg
     3481 aatccgctgg cacctgaatt tatcccccgg gctctaagac tgcagcattc aggaagtacc
     3541 aacaaacagc agcaatcacc acccaagggg aaaagtcttc atcataccca gaatgatcac
     3601 ttccagaatg atggaattgt tcagcccaat ccttctgtac ttattggcaa tcctattaga
     3661 gcatatactc ctccaccccc tcttggacct cacccaaatt tgggaaaatc tccaagccct
     3721 gttcaaagaa tagatcctca cactgggaca agtattcttt atgtacctgc tgtctatgga
     3781 gggaatgtag ttatgtcggt gcctttacct gtaccatgga caggatacca gggtaggttt
     3841 gcagttgatc ctcgaattat tacacatcag gcagcaatgg cctataacat gaacctatta
     3901 cagacacatg gacgaggatc tcctattcct tatggccttg gacatcaccc acctgtcacc
     3961 ataggccagc cacaaaatca gcatcaggag aaggatcaac atgagcaaaa tcgaaatggt
     4021 aaaagtgata caaataattc cggacctgaa attaataaga ttcgaacacc agagaaaaag
     4081 ccaacagaac caaaacaggt tgatttggaa tcaaatccac agaacagaag tcctgaatca
     4141 cgtcctagtg ttgtttatcc cagtaccaaa tttcctcgca aagataatct caacccaaga
     4201 cacataaatc ttccccttcc tgctccccac gcacagtatg caatccctaa tcgccacttt
     4261 catccccttc cccagctacc aagaccaccc tttccaattc cacagcagca caccttgtta
     4321 aatcagcagc agaataattt gcctgaacaa ccaaatcaga taccacctca gccaaatcag
     4381 gtagtccagc agcaaagtca gttgaatcag cagcctcagc agccacctcc tcagctttct
     4441 cctgcatatc aggcgggacc caacaatgct ttttttaata gtgcagttgc tcatcggcca
     4501 cagtctcctc ctgcagaagc tgtaattccg gagcagcagc cccctcccat gctgcaagaa
     4561 ggccacagtc ctctgagagc cattgcacaa cccggcccca ttcttccttc acatctgaat
     4621 agcttcattg atgagaaccc ctcgggatta cctatagggg aggctttaga tcgtatacat
     4681 gggagtgtcg ctctggaaac attaaggcag cagcaggcac ggttccagca gtggagcgag
     4741 catcatgcct ttctcagtca gggcagcgct ccatacccac accatcacca tcctcacctc
     4801 cagcatcttc ctcagccgcc cctgggatta catcagccgc cagtgagggc agactggaag
     4861 ctcaccagca gtgccgaaga tgaagtggag accacatact caaggtttca agacttaatc
     4921 agagaactgt ctcatcgtga tcaaagtgaa acacgggaac tagctgaaat gccaccacct
     4981 caatcaagac ttttgcaata tagacaagta cagagtagaa gcccaccagc agtcccatct
     5041 cccccatcca gtacagacca cagtagccac ttttctaact ttaatgataa cagcagagac
     5101 attgaagtag ccagcaaccc agcatttcca cagcgcctcc caccccagat attcaactca
     5161 cctttctcgt tgccatctga acaccttgcc cctcctccct tgaaatacct ggcacctgat
     5221 ggagcatgga cttttgctaa cttgcaacag aatcacctaa tggggccagg ttttccctat
     5281 ggcctacctc cattgcctca caggccaccg cagaaccctt ttgtacaaat acagaatcat
     5341 caacatgcta ttggtcaaga gccatttcac ccattgtcat ctcgaacagt atcttcttct
     5401 tcgctcccta gcttagaaga gtatgagccc agaggacctg gtcggccctt gtaccaaaga
     5461 agaatctcat ctagctcagt tcaaccttgt tctgaagaag taagcactcc tcaagacagt
     5521 ctggctcagt gtaaagagct tcaggaccac agtaaccaat cttctttcaa cttttcatcc
     5581 ccggagtcct gggtaaacac cacctcatct actccttatc agaacattcc gtgcaatgga
     5641 tccagcagga cagctcagcc cagagagttg atagcgccac ccaagactgt caaaccccct
     5701 gaggatcaac tgaagtcgga gaacctcgag gtgtccagtt ccttcaacta cagtgtgctg
     5761 cagcatcttg gccagtttcc accccttatg cctaacaagc agatcgcgga gtcggccaat
     5821 agcagtagcc cccagagctc tgcggggggc aagcccgcca tgtcctatgc cagcgctctg
     5881 cgggcccctc caaagcccag gccccctcct gagcaggcca agaagagtag cgaccctctg
     5941 tctctcttcc aggaactgag cctagggagc tcatctggca gcaatggctt ttactcatat
     6001 tttaaataat cacttttttt ttccctcaag ggagaatgtt ttaatttctg tttgtatcag
     6061 tagaattaag gtagttggac ttcatctata gatgcacagt tccctttgtt ttaatattaa
     6121 atatgttctc acttaattgc tttgctgcta gacttgcaac taattttttt aaagtatatt
     6181 ccattatttt gcatttttga tgtgtcaaaa ctttgacagc ttttatgtag aataaaaaaa
     6241 tttttaaatt tgtgtattgt tacatatgtt tgcatcaagc tagcagccaa gaggttaatt
     6301 gtgcaactat aaaaaaaaaa aaaaaaagaa aagtttgtct aaaatgtatt taaaaagaaa
     6361 aaaaaattgt aagtagcatt tacatttatt caataatatt accacatgct gggtttctgt
     6421 accagaaatg gccagttgat gtacaaatgt atgttatttt tgcttaaatt catttaaatt
     6481 ttttttaaaa taaaggggca gcatcttcta aataagtttt atcagctttt tttttttttt
     6541 tggtgacacg atgctgcatt ctaaaacttt tatagtttta gactatgtat gatgtgttgt
     6601 tgtaatctcc atccaccata ggatagagtt aaaggcattc tttgcaggca tattttgtat
     6661 gccactgttt tttgaaatgt gaaaaaaacg ttggcatact tagttatttt ttggacaagc
     6721 tacacttcaa gcttgttttt aatgttaatt tgatagtgtg tgtttttttt tttaaacaaa
     6781 tcaagaagct gaaaaatttt taggattgtt ggtgcttagt agacttgata actattttaa
     6841 aaatgcgtga atttagtaaa attctttttt tctcactatg catgtgtaaa tgtttgtaac
     6901 ctggcttccc tcttctccag tggtataaag aattgtgccg ctttaaggtt tgggtttttt
     6961 tctctccagt aaaaattttg gtaccttatt tgatgtttac attaaaatgt gacattatac
     7021 gtggcaattc aaatattttg ctagctgtct tgtttgagga acaccattaa agaagaaata
     7081 taatgtttgt caaaaatgta tcaaaattca tcagaatctg gggcagaaaa ctacccttgt
     7141 ttttcatgtc tttcaattct tctaaaatac cccttattga acttgtacac aatataaaat
     7201 ttaaatagcc atcttaaata ttctaccatc taaaaattgc tttgaatgaa ttttggtaaa
     7261 atccaaagga agatgtttac cctcttgaaa cttatggtct tgtcacacat ttaaagcaat
     7321 atatgtcctt attttcctga ccttgtaata ctacttttat atttcagagg tatctcgaaa
     7381 ctacaatata aacttttaaa aaaaaataag atgaggctgg ggaacctttc gaatgaaccc
     7441 aatcttcctg atgatccatt caaaaacagg tttttggaag accactttcg ataggcaatc
     7501 ctataatcaa agtacatttt taaaatcagg ttgtaaaatg gcaaaggtaa agaaaaatta
     7561 ctgacatttc ttctatgatt ggagtttagt ttatgttggc cagctacaac cccttttaat
     7621 cctcagacag tttgggataa aagagtgcct ttggttaatg gttagcaagc tccaggataa
     7681 gcgctgcgtg cctcttgtat tcagctctac ctttctgcta agagtgcggt tcatttgtgt
     7741 gtggctttgc gttccaggct gtgggtctct gtgtaaggtg gcagacatag acttgtaaac
     7801 tgcagacgtg ttaatggtta gcataaatca cagctggaaa actgagccgg caagttacag
     7861 caccactctt tctctgtatg atgtcagatc accacattta gcaatgtcaa attttactca
     7921 acattcttta gtaggaagaa aagaagaaaa agttttgcca cgtagctatg tgcttttaag
     7981 tctgtgacaa aactcaagac atatccgaat gaaaattaga tctctctgat gtgaggatgt
     8041 tatggaaaat aattttccca tcagtgatta tccacccccc agcccccata tacaggacta
     8101 caacgcaagc tagataaatg ttcaaaagca atctgataat atgaagacag aattgtcaag
     8161 tcagggcttt cagttgaccc cttgtcatga ggttattgcc taattttctg ttttctatac
     8221 ttgaatcttt aaaaaatatc agtttttaaa ttatttatat ttatatttag ttaagggtca
     8281 tggggtcgtg tatgcacccc atcaccttat ataaatatgt tgatgatgga agctttggat
     8341 aaggaagcta ttaagttgtc atgacatcct tgttctaagc agttatttct aaaaactaaa
     8401 aataatagct acttattggc ccaccgctct gctgtgagta tatgagtact tctcattcct
     8461 tatttccata gccagtcaag gattctaacg ctgcttccat ctgatactct tcttagattc
     8521 tgcatcaaat gcttattttg acttacaaca agcttactct tcctcagcat tacataatgc
     8581 catctttcca accatgcttt aaagaacctg ctctttacgc atagtatctt ataacatttt
     8641 gaacacgtct tgtgtaagaa tcattgcaag tttccatcaa aatgaggcgt gtcttctgag
     8701 tacagctgat gttctttggg tattacttga gtagtagtag caagtaccca taaatcagat
     8761 ttatgaaaca taacttatat gctaggttca acccttatat atgcatatat atgtatagta
     8821 tatgtatata aaattttttt ccagaaaact tgatgatagc tctcagataa taccaagtat
     8881 taccagcttc catttcttca gagcagattt ttttctaagt gaggttaatt gttcacataa
     8941 tgtcttttta catctttgtc ctttgtatca ctctgaagtt ttctttttct tgccagaata
     9001 tttgtttcac tttttaattt cagtgctact tgagattttc ttttttttta acctgaaaga
     9061 cgatattttg gatgatagag tttgtaaagt gagccttcat gctgaaaaaa ttcttttcat
     9121 gatctaaaaa tggtcttttg ttacgatttg aagaacctca ttccgtgtaa catggatatt
     9181 ttcttgtgtg tctttaaagg gggttttggt tgtcatttgg gcatatgctt tgccaaattg
     9241 ttatagcaga actttgtttt tgattaatca gtagttaatt attgaccatt taaactaaca
     9301 tgaccttaat tttcctttcc tcacacagat gtgctttcta tccaggtcat tttttacacg
     9361 tgaaataaca aactgctttg tgtagatttt tttttttttt aaatcatcag attttagaat
     9421 aagtttaggt ttaatgtaaa catcaactgg tcttttcagt tcagagaact gcagaataag
     9481 taaaataagc agaattccca ctgaataagc attacctaat taaacatagc aacatctaag
     9541 accaatttat gaaaatactc tcctttttta tggaggcatt ggagctgctg agaaagctaa
     9601 tgaatttccc aactttggga taattaatat atagtattgt gttgctctag tgtagttgat
     9661 tttatgtgtt taatattttt catgtcaaga gcaaagctgc agacgtaaaa gatgtttctc
     9721 tagccccctt agctagagcg agccaccttt gttaagagtc agggtgtgaa gaacaatttc
     9781 aagataaatt ttagtatttc aaccattgtt attaagaaaa ttagacttac tgaaatactc
     9841 atttaaaaac agacaccttc tatagcattg ttttatagtg gagttatttt taaaaaggga
     9901 atcccagaaa gaatctataa aaatacaagg ttaaaacttt aaatgtattg aaagatacaa
     9961 attggtgctt tcaaatgtca tgtaagctag ccattgtgtt tttttttttc cctccagctc
    10021 ctcaatggta gtaatttgtc ttttatgatt taccgtacat tatttcttgc tcaccatccc
    10081 cttaacatat gaaaatcctt aatttctact gccgcgctat atgtcttcct gaatctcaag
    10141 gtataaatgt cttaatggat ctgatttgtc tccactcttt gccatctgat ccacattcaa
    10201 accgcctaga aattagaaag aataggtaat gttctatcta cgatttcttg ttcaaaatat
    10261 cacgtagtgg tgccaatcac ttacaaagac accatctcca tcttgaaaga gtaactggca
    10321 tgttagtctt gaaaatgctc attatgatat gctcctcaca tttcccatct gcttgaggat
    10381 ggacatttat tagctgagac tttaaagctg ctaaattcac actttctact ttcatacctt
    10441 tttaatcagt aatgtcaaga ccattgttgc aaggagtgaa tcttactaaa cacatcctgt
    10501 tattacttta aactagaata tgagattcaa gagatttaaa aacaatttta atgtaacgtt
    10561 taaaattttt gaaccaattg agaatgtcta ctttggagtg aagtttaagg aaacagtcac
    10621 cagagtttct aaaaattttt tggagggagg tacctaatag aagaatctag acttagaaat
    10681 gtgaactgtg gttaaatacc aaacccagtt ccgcagtggt cgtctaaagc gtctaaaact
    10741 ggagtgttaa gaaaacccag agacattcat atagtttcag ttatcagagt attagaccag
    10801 actttatttg caatttgttc aacagagaga ttttattttg atacagatat taagcataaa
    10861 gcatagtaag ttttatataa ttctatgagt tcattaaaca atattatgaa attttcatta
    10921 tgttcgtggg gagtcttttt aactgtttct acctcatata tgaggtctct tctgtatttt
    10981 actttgcata ctagcatgtg attgcctgcc ttagaactat gtagaggagg ggaaatcgat
    11041 attacctttt aagaaatttt tgattttatg aagtgttttc attaatactt ttttaatgtt
    11101 tgagaagtaa aggggaaagg gtggcaaatt acataggttg caacacagag tagaagtcac
    11161 atagtactat ttacagatct gtcctgtttt aagatttgag aattgtgaac aggatggagc
    11221 aatggttctc aaacttcagc cagtatcaga attgcctcga ggtcttgtta aaattcagat
    11281 agctggaccc acccccagca tttctgattt tggtctggag cctgatgatt tgcatttcca
    11341 acaagtttcc aggcgatgct gatgctgcca gtccaggaac cacactttga aaatcactgg
    11401 gtctgattct cgcagtttga aagtctcaga gaccttcaga gtctgaacgt cttttcaacc
    11461 atgtatttat ttactcagtg taaaaaactg atctctggag acatttttat aaatctggtg
    11521 ccatgactgg aacatttgtg gagaaaggtc tccatggagc gacaggccct ttggattggc
    11581 caccacccct cctcactctc acccctgctg gggtctgcac tgctcttccg cagtagggtg
    11641 aaggagctct ttggttttag ggtgagtttg cttttgtgta cgctgctgcc tggacatcag
    11701 tctggaagct gtgcatttgt ttggtgtgct tagggtcaat cttgatccac atgcatggaa
    11761 gtgagaatcc tggaaagaag ccatcagaat gccatgagag tgcacctagc atgtatcgag
    11821 gcactaagtg caggagtccc tgtcctaaga tggcagtgac atggagcatc gaaaaccaca
    11881 gataatcctt aaataataaa ataatagttt tgtttttttt tcttcggggc agagtctcgc
    11941 tctgttgccc tggctggagt acagtggcat gatctcggct cactgcaacc tccgcctccc
    12001 ggcttcaagt gattctcctg cctcagcctc ccaagtagtt gggactacag gcatgcgcca
    12061 ccacgcccag ctaatttttg tattttttag tagagacggg gtttcaccgt gttggccagg
    12121 atggtctcca tctcttgacc tcatgatctg cccgcctcgg gctcccaaag tgttgggatt
    12181 acaggcatga gccactgcgc ctggcaaatg ttttagtttt tatggttatt attatttttt
    12241 ttttttgtgg gggagggtgg tgagggcaaa ggatttgttt ttcaaattat gtgtttctca
    12301 ggttaacaaa gttattttag attctctgcc atgccagcag gtgttagaga gagcctgaag
    12361 tgttacatat ggcagaaaat aaatgtctct gattactttg ctacctttaa aaaaatctat
    12421 atgtgtttgc aaaacagcct agggggatct accacctaca cagcatgaat tattcataag
    12481 tcataggtgc acatgtatga gcaagttatt tttgagaaag aaactgccta taatataata
    12541 aacctgtcag gtctttgggt attgtttaat ttgtgtgttg ttgctgtttt atctggactg
    12601 catcttgggg ggtactgggc tctgcttctg gtttttagtt tgcctttgaa tttactgagc
    12661 tactagaaag acaaaaaaaa gctattttta tggtaataga atacagaatg acttaggtgt
    12721 aaaagtacac gtgaagacca tcgtagaata tacagctttg gagttttctt aagacacagt
    12781 ccttcctact gaaaaggctt gctcaccttt ggagaggatt gtttatattt tcagcctggg
    12841 ttcctttatg cagtcccaac ttagagggga gcttcggttt atttacactt tgctttgcac
    12901 gataacttgt atgcgaagac ctctttatat ctttcagtag gtcagagggc tctttgtcct
    12961 ggttgaagtc ccaaagtgtc agtgcagctg agaatgtttc tcagcctccc cctttacaga
    13021 ggaagggtcc tggattttca taaatccaga ccatgcctgc ttcctataaa tccatgtata
    13081 tgacattgta ctgtatctga tttctgtgcc agtcatctga cctgattctt taaaccccat
    13141 aaaggtactg gctatatgta gtagttgtat cctaggtaaa tatggtgatt aaaatacttc
    13201 tcttctaaat tataaagata atgagttttg cattttatta aagggtcctg ttattagtga
    13261 cacaggattt ttttttaatg cttcatacat atagttctta tctaaacttg ttcagctaac
    13321 ttcatagatc agttacttag caaattcata ctgagtctta ttttgggaag tcttcctgag
    13381 ggaagctgta caaaactgag gtggtcaggt tcatctcttc aaagtaccag ttatacagcc
    13441 atctaaatag gcataactta tggcaaaatt ataacaattt tttttctttg cagtaaatga
    13501 tacctcatct aagaggctct aatacctaaa gagtttatcc ttaaaagtaa aagtgacttt
    13561 gtaccataaa ataaccccaa agccactctc tagggttttt attttcttct ttctttgtct
    13621 ttatcttttc taacttagtt ttggaattac gttagctact ttggttccat agaacaagtt
    13681 ttcaacttgg ggtctacagt tcctacagtg tgctcattgt ctcagtgaag ttcttttgtt
    13741 ttcaaagatt catggtaaca aaatgtattt tactgctaca actaaaatgt atttataata
    13801 aaatgccttt ttaataa
//
