LOCUS       NM_001370285            3472 bp    mRNA    linear   PRI 12-FEB-2025
DEFINITION  Homo sapiens DNA helicase B (HELB), transcript variant 4, mRNA.
ACCESSION   NM_001370285 XM_005269234
VERSION     NM_001370285.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Hormeno,S., Wilkinson,O.J., Aicart-Ramos,C., Kuppa,S., Antony,E.,
            Dillingham,M.S. and Moreno-Herrero,F.
  TITLE     Human HELB is a processive motor protein that catalyzes RPA
            clearance from single-stranded DNA
  JOURNAL   Proc Natl Acad Sci U S A 119 (15), e2112376119 (2022)
   PUBMED   35385349
  REMARK    GeneRIF: Human HELB is a processive motor protein that catalyzes
            RPA clearance from single-stranded DNA.
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   Zafar,M.K., Hazeslip,L., Chauhan,M.Z. and Byrd,A.K.
  TITLE     The Expression of Human DNA Helicase B Is Affected by
            G-Quadruplexes in the Promoter
  JOURNAL   Biochemistry 59 (26), 2401-2409 (2020)
   PUBMED   32478505
  REMARK    GeneRIF: The Expression of Human DNA Helicase B Is Affected by
            G-Quadruplexes in the Promoter.
REFERENCE   3  (bases 1 to 3472)
  AUTHORS   Hazeslip,L., Zafar,M.K., Chauhan,M.Z. and Byrd,A.K.
  TITLE     Genome Maintenance by DNA Helicase B
  JOURNAL   Genes (Basel) 11 (5), 578 (2020)
   PUBMED   32455610
  REMARK    GeneRIF: Genome Maintenance by DNA Helicase B.
            Review article
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3472)
  AUTHORS   Tkac,J., Xu,G., Adhikary,H., Young,J.T.F., Gallo,D.,
            Escribano-Diaz,C., Krietsch,J., Orthwein,A., Munro,M., Sol,W.,
            Al-Hakim,A., Lin,Z.Y., Jonkers,J., Borst,P., Brown,G.W.,
            Gingras,A.C., Rottenberg,S., Masson,J.Y. and Durocher,D.
  TITLE     HELB Is a Feedback Inhibitor of DNA End Resection
  JOURNAL   Mol Cell 61 (3), 405-418 (2016)
   PUBMED   26774285
  REMARK    GeneRIF: Conclude that mammalian DNA end resection triggers its own
            inhibition via the recruitment of HELB.
REFERENCE   5  (bases 1 to 3472)
  AUTHORS   Gerhardt,J., Guler,G.D. and Fanning,E.
  TITLE     Human DNA helicase B interacts with the replication initiation
            protein Cdc45 and facilitates Cdc45 binding onto chromatin
  JOURNAL   Exp Cell Res 334 (2), 283-293 (2015)
   PUBMED   25933514
  REMARK    GeneRIF: The depletion of HDHB from human cells diminishes Cdc45
            association with chromatin, suggesting that HDHB may facilitate
            Cdc45 recruitment at G1/S in human cells.
REFERENCE   6  (bases 1 to 3472)
  AUTHORS   Liu,H., Yan,P. and Fanning,E.
  TITLE     Human DNA helicase B functions in cellular homologous recombination
            and stimulates Rad51-mediated 5'-3' heteroduplex extension in vitro
  JOURNAL   PLoS One 10 (1), e0116852 (2015)
   PUBMED   25617833
  REMARK    GeneRIF: HDHB promotes homologous recombination in vivo and
            stimulates 5'-3' heteroduplex extension during Rad51-mediated
            strand exchange in vitro
            Erratum:[PLoS One. 2018 Oct 10;13(10):e0205766. doi:
            10.1371/journal.pone.0205766.. PMID: 30304027]
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 3472)
  AUTHORS   Guler,G.D., Liu,H., Vaithiyalingam,S., Arnett,D.R., Kremmer,E.,
            Chazin,W.J. and Fanning,E.
  TITLE     Human DNA helicase B (HDHB) binds to replication protein A and
            facilitates cellular recovery from replication stress
  JOURNAL   J Biol Chem 287 (9), 6469-6481 (2012)
   PUBMED   22194613
  REMARK    GeneRIF: Replication stress-induced recruitment of HDHB to
            chromatin is independent of checkpoint signaling but correlates
            with the level of replication protein A (RPA) recruited to
            chromatin.
REFERENCE   8  (bases 1 to 3472)
  AUTHORS   Gu,J., Xia,X., Yan,P., Liu,H., Podust,V.N., Reynolds,A.B. and
            Fanning,E.
  TITLE     Cell cycle-dependent regulation of a human DNA helicase that
            localizes in DNA damage foci
  JOURNAL   Mol Biol Cell 15 (7), 3320-3332 (2004)
   PUBMED   15146062
REFERENCE   9  (bases 1 to 3472)
  AUTHORS   Taneja,P., Gu,J., Peng,R., Carrick,R., Uchiumi,F., Ott,R.D.,
            Gustafson,E., Podust,V.N. and Fanning,E.
  TITLE     A dominant-negative mutant of human DNA helicase B blocks the onset
            of chromosomal DNA replication
  JOURNAL   J Biol Chem 277 (43), 40853-40861 (2002)
   PUBMED   12181327
  REMARK    GeneRIF: role of dominant-negative mutant in blocking onset of
            chromosomal DNA replication
REFERENCE   10 (bases 1 to 3472)
  AUTHORS   Tada,S., Kobayashi,T., Omori,A., Kusa,Y., Okumura,N., Kodaira,H.,
            Ishimi,Y., Seki,M. and Enomoto,T.
  TITLE     Molecular cloning of a cDNA encoding mouse DNA helicase B, which
            has homology to Escherichia coli RecD protein, and identification
            of a mutation in the DNA helicase B from tsFT848
            temperature-sensitive DNA replication mutant cells
  JOURNAL   Nucleic Acids Res 29 (18), 3835-3840 (2001)
   PUBMED   11557815
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC078889.20.
            
            On May 4, 2019 this sequence version replaced XM_005269234.2.
            
            Summary: This gene encodes a DNA-dependent ATPase which catalyzes
            the unwinding of DNA necessary for DNA replication, repair,
            recombination, and transcription. This gene is thought to function
            specifically during the S phase entry of the cell cycle.
            Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Feb 2016].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660805.41586.1, AF319995.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000247815.9/ ENSP00000247815.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-298               AC078889.20        68021-68318         c
            299-718             AC078889.20        65661-66080         c
            719-888             AC078889.20        64297-64466         c
            889-1791            AC078889.20        60203-61105         c
            1792-1969           AC078889.20        56648-56825         c
            1970-2111           AC078889.20        55428-55569         c
            2112-2266           AC078889.20        52019-52173         c
            2267-2348           AC078889.20        48782-48863         c
            2349-2408           AC078889.20        48028-48087         c
            2409-2637           AC078889.20        46600-46828         c
            2638-2781           AC078889.20        45685-45828         c
            2782-3273           AC078889.20        39166-39657         c
            3274-3472           AC078889.20        32612-32810         c
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q14.3"
     gene            1..3472
                     /gene="HELB"
                     /gene_synonym="DHB; hDHB"
                     /note="DNA helicase B"
                     /db_xref="GeneID:92797"
                     /db_xref="HGNC:HGNC:17196"
                     /db_xref="MIM:614539"
     exon            1..298
                     /gene="HELB"
                     /gene_synonym="DHB; hDHB"
                     /inference="alignment:Splign:2.1.0"
     CDS             112..3375
                     /gene="HELB"
                     /gene_synonym="DHB; hDHB"
                     /EC_number="3.6.4.12"
                     /note="helicase (DNA) B"
                     /codon_start=1
                     /product="DNA helicase B"
                     /protein_id="NP_001357214.1"
                     /db_xref="CCDS:CCDS8976.1"
                     /db_xref="GeneID:92797"
                     /db_xref="HGNC:HGNC:17196"
                     /db_xref="MIM:614539"
                     /translation="MARSSPYLRQLQGPLLPPRDLVEEDDDYLNDDVEEDEESVFIDA
                     EELCSGGVKAGSLPGCLRVSICDENTQETCKVFGRFPITGAWWRVKVQVKPVVGSRSY
                     QYQVQGFPSYFLQSDMSPPNQKHICALFLKECEVSSDDVNKFLTWVKEVSNYKNLNFE
                     NLRETLRTFHKETGRKDQKQPTQNGQEELFLDNEMSLPLENTIPFRNVMTALQFPKIM
                     EFLPVLLPRHFKWIIGSGSKEMLKEIEEILGTHPWKLGFSKITYREWKLLRCEASWIA
                     FCQCESLLQLMTDLEKNALIMYSRLKQICREDGHTYVEVNDLTLTLSNHMSFHAASES
                     LKFLKDIGVVTYEKSCVFPYDLYHAERAIAFSICDLMKKPPWHLCVDVEKVLASIHTT
                     KPENSSDDALNESKPDEVRLENPVDVVDTQDNGDHIWTNGENEINAEISEVQLDQDQV
                     EVPLDRDQVAALEMICSNPVTVISGKGGCGKTTIVSRLFKHIEQLEEREVKKACEDFE
                     QDQNASEEWITFTEQSQLEADKAIEVLLTAPTGKAAGLLRQKTGLHAYTLCQVNYSFY
                     SWTQTMMTTNKPWKFSSVRVLVVDEGSLVSVGIFKSVLNLLCEHSKLSKLIILGDIRQ
                     LPSIEPGNLLKDLFETLKSRNCAIELKTNHRAESQLIVDNATRISRRQFPKFDAELNI
                     SDNPTLPISIQDKTFIFVRLPEEDASSQSSKTNHHSCLYSAVKTLLQENNLQNAKTSQ
                     FIAFRRQDCDLINDCCCKHYTGHLTKDHQSRLVFGIGDKICCTRNAYLSDLLPENISG
                     SQQNNDLDASSEDFSGTLPDFAKNKRDFESNVRLCNGEIFFITNDVTDVTFGKRRSLT
                     INNMAGLEVTVDFKKLMKYCRIKHAWARTIHTFQGSEEQTVVYVVGKAGRQHWQHVYT
                     AVTRGRCRVYVIAEESQLRNAIMKNSFPRKTRLKHFLQSKLSSSGAPPADFPSPRKSS
                     GDSGGPSTPSASPLPVVTDHAMTNDVTWSEASSPDERTLTFAERWQLSSPDGVDTDDD
                     LPKSRASKRTCGVNDDESPSKIFMVGESPQVSSRLQNLRLNNLIPRQLFKPTDNQET"
     exon            299..718
                     /gene="HELB"
                     /gene_synonym="DHB; hDHB"
                     /inference="alignment:Splign:2.1.0"
     exon            719..888
                     /gene="HELB"
                     /gene_synonym="DHB; hDHB"
                     /inference="alignment:Splign:2.1.0"
     exon            889..1791
                     /gene="HELB"
                     /gene_synonym="DHB; hDHB"
                     /inference="alignment:Splign:2.1.0"
     exon            1792..1969
                     /gene="HELB"
                     /gene_synonym="DHB; hDHB"
                     /inference="alignment:Splign:2.1.0"
     exon            1970..2111
                     /gene="HELB"
                     /gene_synonym="DHB; hDHB"
                     /inference="alignment:Splign:2.1.0"
     exon            2112..2266
                     /gene="HELB"
                     /gene_synonym="DHB; hDHB"
                     /inference="alignment:Splign:2.1.0"
     exon            2267..2348
                     /gene="HELB"
                     /gene_synonym="DHB; hDHB"
                     /inference="alignment:Splign:2.1.0"
     exon            2349..2408
                     /gene="HELB"
                     /gene_synonym="DHB; hDHB"
                     /inference="alignment:Splign:2.1.0"
     exon            2409..2637
                     /gene="HELB"
                     /gene_synonym="DHB; hDHB"
                     /inference="alignment:Splign:2.1.0"
     exon            2638..2781
                     /gene="HELB"
                     /gene_synonym="DHB; hDHB"
                     /inference="alignment:Splign:2.1.0"
     exon            2782..3273
                     /gene="HELB"
                     /gene_synonym="DHB; hDHB"
                     /inference="alignment:Splign:2.1.0"
     exon            3274..3472
                     /gene="HELB"
                     /gene_synonym="DHB; hDHB"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3452..3457
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HELB"
                     /gene_synonym="DHB; hDHB"
                     /note="hexamer: GATAAA"
     polyA_site      3472
                     /gene="HELB"
                     /gene_synonym="DHB; hDHB"
                     /note="major polyA site"
ORIGIN      
        1 aggccgttcc cggaagttga tggccttaca gtcgtagaac tgattggctg atcatgacca
       61 tgcagttagc cagggttttc ccgagttgtt tgggttgagt tcaggagaag catggccagg
      121 tcgagtccgt acctgcgcca acttcaggga cctctgctcc cacccaggga tctggtggag
      181 gaggacgacg actacctaaa cgacgacgtg gaggaggatg aagagtccgt gttcatcgac
      241 gccgaggagc tctgcagtgg gggcgtaaag gctggcagcc tccccgggtg cctccgcgtt
      301 tctatttgtg atgaaaacac acaagagaca tgtaaagtgt ttggacgttt tccgataaca
      361 ggtgcttggt ggagagtgaa ggtacaagta aagcctgtgg tgggatcaag gagctatcaa
      421 tatcaagttc aaggatttcc gtcttacttt ttgcagtctg atatgtcacc accaaatcaa
      481 aaacatatct gtgctctctt tcttaaagag tgtgaggtct ccagtgatga tgttaataaa
      541 tttttaacat gggtaaagga ggtatcaaac tacaaaaacc taaactttga aaatcttagg
      601 gaaacactaa gaactttcca caaggaaact ggaaggaaag atcaaaagca gcctacacag
      661 aatggtcagg aagagttgtt cctagacaat gagatgagtc ttcctctgga aaacacaatt
      721 ccatttagaa atgtaatgac agctttgcag tttccgaaga taatggaatt ccttccagtt
      781 cttctgcctc gacactttaa atggatcata gggtcaggtt ctaaagagat gttgaaagag
      841 atagaagaga ttttaggtac acatccgtgg aaacttggat ttagtaaaat aacctacaga
      901 gagtggaaac tcctgcgatg tgaggcaagt tggatagcat tttgtcagtg tgagtctctt
      961 ctccagctga tgactgattt ggagaagaat gcattaataa tgtattccag actgaagcag
     1021 atatgtagag aagatgggca cacatatgtt gaagtgaatg acttaacttt gacattgtca
     1081 aatcatatgt catttcatgc tgcttcagag tctctgaagt ttttgaagga tattggtgtg
     1141 gtgacatatg agaagtcctg tgtcttccct tatgaccttt accatgctga aagagccatc
     1201 gccttttcaa tttgtgacct gatgaagaaa cctccttggc atttatgtgt cgatgtcgaa
     1261 aaggtgcttg cctctattca caccacaaaa cctgagaatt caagcgatga tgcattgaat
     1321 gagagcaaac ctgatgaagt aagattagaa aatcctgtgg atgttgtgga cacacaggac
     1381 aatggtgacc atatttggac taatggtgaa aatgaaatta atgcagaaat aagtgaagtt
     1441 cagctggatc aggatcaggt tgaagttcca ctggatcggg atcaggtggc tgctttggaa
     1501 atgatttgct ccaatcctgt gacagtcata agtgggaaag gtggatgtgg gaagaccaca
     1561 atcgttagcc gtctttttaa gcatatagag cagttggaag aaagagaagt aaaaaaagcc
     1621 tgtgaagatt ttgaacaaga ccagaatgct tcagaagaat ggattacctt tactgagcaa
     1681 agtcaactag aggcggacaa ggctatagaa gttttgctca cagcacctac agggaaagca
     1741 gctggcttac taagacagaa aactggtctt catgcctaca cactgtgtca ggtcaattat
     1801 agcttctatt catggactca aacaatgatg accacaaaca aaccatggaa attttcttcg
     1861 gttagagttc tggttgtgga tgaagggagt ttggtatctg taggaatctt caaatcggtc
     1921 ttaaatttat tgtgtgagca ctccaaactt tctaagctta ttatccttgg tgacattaga
     1981 cagttaccca gtattgaacc tggtaacttg ctgaaagatc tttttgagac tcttaagtca
     2041 agaaattgtg ctattgagct aaagacaaac catagagcag aatctcagct cattgtggac
     2101 aatgctacaa gaatctcaag acgccaattt ccaaaatttg atgcagaact aaatatctct
     2161 gataatccaa cattacccat ctcaattcaa gataagacat ttatttttgt caggctccca
     2221 gaagaggatg ccagttctca gtcatctaaa actaatcatc actcttgttt atattctgca
     2281 gttaaaactt tactacaaga aaataactta caaaatgcaa aaacatcaca atttattgca
     2341 tttagaaggc aagactgtga tctaattaat gactgctgct gcaaacacta cacaggccac
     2401 ctcaccaaag accatcagag tagacttgtt tttggaattg gtgataaaat ttgttgtacc
     2461 aggaatgcat acctctcaga cttactacct gaaaatatct ctggaagtca gcaaaataat
     2521 gatctagatg ccagtagtga agacttttct ggtacgcttc ctgattttgc taaaaataag
     2581 cgtgactttg aaagtaacgt tcgactgtgc aatggagaga tatttttcat aacaaatgat
     2641 gtaactgatg taacttttgg aaagagaaga tctttgacca ttaataatat ggctggcctg
     2701 gaagtaactg tggattttaa gaaactaatg aaatattgtc gcataaaaca tgcatgggca
     2761 agaactattc acacttttca ggggtccgag gagcaaacag ttgtctatgt ggtggggaag
     2821 gcgggccgcc agcactggca gcatgtctac accgccgtga ccaggggccg ctgccgagtg
     2881 tatgtgattg cagaggagtc tcagctccgg aatgccatta tgaaaaacag ttttcctaga
     2941 aaaactcgtt tgaaacattt cttgcaaagt aagctctcct ctagcggcgc acctccagca
     3001 gattttccgt ccccacggaa gagctctgga gacagtggag gacccagcac accgtcagca
     3061 tctccactcc ctgtagtcac agaccacgcc atgacaaatg atgtcacctg gagcgaggcc
     3121 tcttcgcctg atgagaggac actcaccttt gctgaaagat ggcaattatc ttcacctgat
     3181 ggagtagata cagatgatga tttaccaaaa tcgcgagcat ccaaaagaac ctgtggtgtg
     3241 aatgatgatg aaagtccaag caaaattttt atggtgggag aatctccaca agtgtcttcc
     3301 agacttcaga atttgagact gaataattta attcccaggc aacttttcaa gcccaccgat
     3361 aatcaagaaa cttagtttta tttcaaattg ttccgagtaa ctatgttttt ctattggaga
     3421 caaaatgaac atcgtaacgt caaagtacca agataaaaaa agtttcctat aa
//
