LOCUS       NM_020733               9195 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens heart development protein with EGF like domains 1
            (HEG1), mRNA.
ACCESSION   NM_020733 XM_001129251 XM_087386
VERSION     NM_020733.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9195)
  AUTHORS   Tamargo,I.A., Baek,K.I., Xu,C., Kang,D.W., Kim,Y., Andueza,A.,
            Williams,D., Demos,C., Villa-Roel,N., Kumar,S., Park,C., Choi,R.,
            Johnson,J., Chang,S., Kim,P., Tan,S., Jeong,K., Tsuji,S. and Jo,H.
  TITLE     HEG1 Protects Against Atherosclerosis by Regulating Stable
            Flow-Induced KLF2/4 Expression in Endothelial Cells
  JOURNAL   Circulation 149 (15), 1183-1201 (2024)
   PUBMED   38099436
  REMARK    GeneRIF: HEG1 Protects Against Atherosclerosis by Regulating Stable
            Flow-Induced KLF2/4 Expression in Endothelial Cells.
REFERENCE   2  (bases 1 to 9195)
  AUTHORS   Hiroshima,K., Wu,D., Koh,E., Sekine,Y., Ozaki,D., Yusa,T.,
            Nakazawa,T., Tsuji,S., Miyagi,Y., Walts,A.E., Marchevsky,A.M.,
            Husain,A.N. and Imai,K.
  TITLE     Membranous HEG1 expression is a useful marker in the differential
            diagnosis of epithelioid and biphasic malignant mesothelioma versus
            carcinomas
  JOURNAL   Pathol Int 71 (9), 604-613 (2021)
   PUBMED   34240508
  REMARK    GeneRIF: Membranous HEG1 expression is a useful marker in the
            differential diagnosis of epithelioid and biphasic malignant
            mesothelioma versus carcinomas.
REFERENCE   3  (bases 1 to 9195)
  AUTHORS   Chen,P.P., Zhang,Z.S., Wu,J.C., Zheng,J.F. and Lin,F.
  TITLE     LncRNA SNHG12 promotes proliferation and epithelial mesenchymal
            transition in hepatocellular carcinoma through targeting HEG1 via
            miR-516a-5p
  JOURNAL   Cell Signal 84, 109992 (2021)
   PUBMED   33774129
  REMARK    GeneRIF: LncRNA SNHG12 promotes proliferation and epithelial
            mesenchymal transition in hepatocellular carcinoma through
            targeting HEG1 via miR-516a-5p.
REFERENCE   4  (bases 1 to 9195)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 9195)
  AUTHORS   Fujii,T., Itami,H., Uchiyama,T., Morita,K., Nakai,T.,
            Hatakeyama,K., Sugimoto,A., Shimada,K., Tsuji,S. and Ohbayashi,C.
  TITLE     HEG1-responsive microRNA-23b regulates cell proliferation in
            malignant mesothelioma cells
  JOURNAL   Biochem Biophys Res Commun 526 (4), 927-933 (2020)
   PUBMED   32284171
  REMARK    GeneRIF: HEG1-responsive microRNA-23b regulates cell proliferation
            in malignant mesothelioma cells.
REFERENCE   6  (bases 1 to 9195)
  AUTHORS   Zheng,X., Riant,F., Bergametti,F., Myers,C.D., Tang,A.T.,
            Kleaveland,B., Pan,W., Yang,J., Tournier-Lasserve,E. and Kahn,M.L.
  TITLE     Cerebral cavernous malformations arise independent of the heart of
            glass receptor
  JOURNAL   Stroke 45 (5), 1505-1509 (2014)
   PUBMED   24643410
  REMARK    GeneRIF: Human patients with cerebral cavernous malformations who
            lack exonic mutations in krev interaction trapped protein (KRIT)1,
            cerebral cavernous malformation (CCM) protein 2, or programmed cell
            death (PDCD)10 protein do not have mutations in HEG.
REFERENCE   7  (bases 1 to 9195)
  AUTHORS   Gingras,A.R., Puzon-McLaughlin,W. and Ginsberg,M.H.
  TITLE     The structure of the ternary complex of Krev interaction trapped 1
            (KRIT1) bound to both the Rap1 GTPase and the heart of glass (HEG1)
            cytoplasmic tail
  JOURNAL   J Biol Chem 288 (33), 23639-23649 (2013)
   PUBMED   23814056
  REMARK    GeneRIF: These data show that HEG1 can recruit the Rap1-KRIT
            complex to the plasma membrane.
REFERENCE   8  (bases 1 to 9195)
  AUTHORS   Gingras,A.R., Liu,J.J. and Ginsberg,M.H.
  TITLE     Structural basis of the junctional anchorage of the cerebral
            cavernous malformations complex
  JOURNAL   J Cell Biol 199 (1), 39-48 (2012)
   PUBMED   23007647
REFERENCE   9  (bases 1 to 9195)
  AUTHORS   Kleaveland,B., Zheng,X., Liu,J.J., Blum,Y., Tung,J.J., Zou,Z.,
            Sweeney,S.M., Chen,M., Guo,L., Lu,M.M., Zhou,D., Kitajewski,J.,
            Affolter,M., Ginsberg,M.H. and Kahn,M.L.
  TITLE     Regulation of cardiovascular development and integrity by the heart
            of glass-cerebral cavernous malformation protein pathway
  JOURNAL   Nat Med 15 (2), 169-176 (2009)
   PUBMED   19151727
  REMARK    Erratum:[Nat Med. 2009 May;15(5):584. Sweeney, Shawn M [added]]
REFERENCE   10 (bases 1 to 9195)
  AUTHORS   Mably,J.D., Mohideen,M.A., Burns,C.G., Chen,J.N. and Fishman,M.C.
  TITLE     heart of glass regulates the concentric growth of the heart in
            zebrafish
  JOURNAL   Curr Biol 13 (24), 2138-2147 (2003)
   PUBMED   14680629
  REMARK    GeneRIF: Identification of the zebrafish heart of glass (heg) gene.
            Description of HEG's role in the regulation of concentric heart
            growth in zebrafish.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC117488.11, DR003209.1, AB033063.2, AC092983.13 and AC026342.34.
            
            On Nov 23, 2018 this sequence version replaced NM_020733.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMEA1965299, SAMEA1966682
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000311127.9/ ENSP00000311502.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-39                AC117488.11        14546-14584         c
            40-498              DR003209.1         8-466
            499-3044            AB033063.2         55-2600
            3045-3045           AC092983.13        32146-32146         c
            3046-3222           AB033063.2         2602-2778
            3223-3223           AC092983.13        29287-29287         c
            3224-4957           AB033063.2         2780-4513
            4958-9195           AC026342.34        12553-16790
FEATURES             Location/Qualifiers
     source          1..9195
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q21.2"
     gene            1..9195
                     /gene="HEG1"
                     /gene_synonym="HEG; MST112; MSTP112"
                     /note="heart development protein with EGF like domains 1"
                     /db_xref="GeneID:57493"
                     /db_xref="HGNC:HGNC:29227"
                     /db_xref="MIM:614182"
     exon            1..423
                     /gene="HEG1"
                     /gene_synonym="HEG; MST112; MSTP112"
                     /inference="alignment:Splign:2.1.0"
     CDS             108..4253
                     /gene="HEG1"
                     /gene_synonym="HEG; MST112; MSTP112"
                     /note="protein HEG homolog 1; heart of glass"
                     /codon_start=1
                     /product="protein HEG homolog 1 precursor"
                     /protein_id="NP_065784.1"
                     /db_xref="CCDS:CCDS46898.1"
                     /db_xref="GeneID:57493"
                     /db_xref="HGNC:HGNC:29227"
                     /db_xref="MIM:614182"
                     /translation="MASPRASRWPPPLLLLLLPLLLLPPAAPGTRDPPPSPARRALSL
                     APLAGAGLELQLERRPEREPPPTPPRERRGPATPGPSYRAPEPGAATQRGPSGRAPRG
                     GSADAAWKHWPESNTEAHVENITFYQNQEDFSTVSSKEGVMVQTSGKSHAASDAPENL
                     TLLAETADARGRSGSSSRTNFTILPVGYSLEIATALTSQSGNLASESLHLPSSSSEFD
                     ERIAAFQTKSGTASEMGTERAMGLSEEWTVHSQEATTSAWSPSFLPALEMGELTTPSR
                     KRNSSGPDLSWLHFYRTAASSPLLDLSSSSESTEKLNNSTGLQSSSVSQTKTMHVATV
                     FTDGGPRTLRSLTVSLGPVSKTEGFPKDSRIATTSSSVLLSPSAVESRRNSRVTGNPG
                     DEEFIEPSTENEFGLTSLRWQNDSPTFGEHQLASSSEVQNGSPMSQTETVSRSVAPMR
                     GGEITAHWLLTNSTTSADVTGSSASYPEGVNASVLTQFSDSTVQSGGSHTALGDRSYS
                     ESSSTSSSESLNSSAPRGERSIAGISYGQVRGTAIEQRTSSDHTDHTYLSSTFTKGER
                     ALLSITDNSSSSDIVESSTSYIKISNSSHSEYSSFFHAQTERSNISSYDGEYAQPSTE
                     SPVLHTSNLPSYTPTINMPNTSVVLDTDAEFVSDSSSSSSSSSSSSSSGPPLPLPSVS
                     QSHHLFSSILPSTRASVHLLKSTSDASTPWSSSPSPLPVSLTTSTSAPLSVSQTTLPQ
                     SSSTPVLPRARETPVTSFQTSTMTSFMTMLHSSQTADLKSQSTPHQEKVITESKSPSL
                     VSLPTESTKAVTTNSPLPPSLTESSTEQTLPATSTNLAQMSPTFTTTILKTSQPLMTT
                     PGTLSSTASLVTGPIAVQTTAGKQLSLTHPEILVPQISTEGGISTERNRVIVDATTGL
                     IPLTSVPTSAKEMTTKLGVTAEYSPASRSLGTSPSPQTTVVSTAEDLAPKSATFAVQS
                     STQSPTTVSSSASVNSCAVNPCLHNGECVADNTSRGYHCRCPPSWQGDDCSVDVNECL
                     SNPCPSTAMCNNTQGSFICKCPVGYQLEKGICNLVRTFVTEFKLKRTFLNTTVEKHSD
                     LQEVENEITKTLNMCFSALPSYIRSTVHASRESNAVVISLQTTFSLASNVTLFDLADR
                     MQKCVNSCKSSAEVCQLLGSQRRIFRAGSLCKRKSPECDKDTSICTDLDGVALCQCKS
                     GYFQFNKMDHSCRACEDGYRLENETCMSCPFGLGGLNCGNPYQLITVVIAAAGGGLLL
                     ILGIALIVTCCRKNKNDISKLIFKSGDFQMSPYAEYPKNPRSQEWGREAIEMHENGST
                     KNLLQMTDVYYSPTSVRNPELERNGLYPAYTGLPGSRHSCIFPGQYNPSFISDESRRR
                     DYF"
     exon            424..717
                     /gene="HEG1"
                     /gene_synonym="HEG; MST112; MSTP112"
                     /inference="alignment:Splign:2.1.0"
     exon            718..1020
                     /gene="HEG1"
                     /gene_synonym="HEG; MST112; MSTP112"
                     /inference="alignment:Splign:2.1.0"
     exon            1021..1359
                     /gene="HEG1"
                     /gene_synonym="HEG; MST112; MSTP112"
                     /inference="alignment:Splign:2.1.0"
     exon            1360..1695
                     /gene="HEG1"
                     /gene_synonym="HEG; MST112; MSTP112"
                     /inference="alignment:Splign:2.1.0"
     exon            1696..3063
                     /gene="HEG1"
                     /gene_synonym="HEG; MST112; MSTP112"
                     /inference="alignment:Splign:2.1.0"
     exon            3064..3180
                     /gene="HEG1"
                     /gene_synonym="HEG; MST112; MSTP112"
                     /inference="alignment:Splign:2.1.0"
     exon            3181..3300
                     /gene="HEG1"
                     /gene_synonym="HEG; MST112; MSTP112"
                     /inference="alignment:Splign:2.1.0"
     exon            3301..3404
                     /gene="HEG1"
                     /gene_synonym="HEG; MST112; MSTP112"
                     /inference="alignment:Splign:2.1.0"
     exon            3405..3463
                     /gene="HEG1"
                     /gene_synonym="HEG; MST112; MSTP112"
                     /inference="alignment:Splign:2.1.0"
     exon            3464..3624
                     /gene="HEG1"
                     /gene_synonym="HEG; MST112; MSTP112"
                     /inference="alignment:Splign:2.1.0"
     exon            3625..3759
                     /gene="HEG1"
                     /gene_synonym="HEG; MST112; MSTP112"
                     /inference="alignment:Splign:2.1.0"
     exon            3760..3802
                     /gene="HEG1"
                     /gene_synonym="HEG; MST112; MSTP112"
                     /inference="alignment:Splign:2.1.0"
     exon            3803..3840
                     /gene="HEG1"
                     /gene_synonym="HEG; MST112; MSTP112"
                     /inference="alignment:Splign:2.1.0"
     exon            3841..3928
                     /gene="HEG1"
                     /gene_synonym="HEG; MST112; MSTP112"
                     /inference="alignment:Splign:2.1.0"
     exon            3929..4103
                     /gene="HEG1"
                     /gene_synonym="HEG; MST112; MSTP112"
                     /inference="alignment:Splign:2.1.0"
     exon            4104..9195
                     /gene="HEG1"
                     /gene_synonym="HEG; MST112; MSTP112"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9174..9179
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HEG1"
                     /gene_synonym="HEG; MST112; MSTP112"
                     /note="hexamer: AATAAA"
     polyA_site      9195
                     /gene="HEG1"
                     /gene_synonym="HEG; MST112; MSTP112"
                     /note="major polyA site"
ORIGIN      
        1 actccgctca cttccccggc cccgcgcggc ccccgccgcc cgctgcccgc tgcccgctgc
       61 cctcgccccg cgcgccgggc atgtgagcgc gggcgggcgc cgtcaccatg gcctcgccgc
      121 gcgcctcgcg gtggccgccg ccgctcctgc tgctgttgct gccgctgctg ctgctgccgc
      181 cggcggcccc cgggacgcgg gacccgccgc cttccccggc tcgccgcgcg ctgagcctgg
      241 cgcccctcgc gggagcgggg ctggagctgc agctggagcg ccgcccggag cgcgagccgc
      301 cgcccacgcc gccccgggag cgccgcgggc ccgcgacccc cggccccagc tacagggccc
      361 ctgagccagg cgccgcgaca cagcggggac cctccggccg ggcccccaga ggcgggagcg
      421 cggatgctgc ctggaaacat tggccagaaa gtaacactga ggcccatgta gaaaacatca
      481 ccttctatca gaatcaagag gacttttcaa cagtgtcctc caaagagggc gtgatggttc
      541 agacctctgg gaagagccat gctgcttcgg atgctccaga aaacctcact ctactcgctg
      601 aaacagcaga tgctagagga aggagcggct cttcaagtag aacaaacttc accattttgc
      661 ctgttgggta ctcactggag atagcaacag ctctgacttc ccagagtggc aacttagcct
      721 cagaaagtct tcacctgcca tccagcagtt cagagttcga tgaaagaatt gccgcttttc
      781 aaacaaagag tggaacagcc tcggagatgg gaacagagag ggcgatgggg ctgtcagaag
      841 aatggactgt gcacagccaa gaggccacca cttcggcttg gagcccgtcc tttcttcctg
      901 ctttggagat gggagagctg accacgcctt ctaggaagag aaattcctca ggaccagatc
      961 tctcctggct gcatttctac aggacagcag cttcctctcc tctcttagac ctttcctcat
     1021 cttctgaaag tacagagaag cttaacaact ccactggcct ccagagctcc tcagtcagtc
     1081 aaacaaagac aatgcatgtt gccaccgtgt tcactgatgg tggcccgaga acgctgcgat
     1141 ctttgacggt cagtctggga cctgtgagca agacagaagg cttccccaag gactccagaa
     1201 ttgccacgac ttcatcctca gtccttcttt caccctctgc agtggaatcg agaagaaaca
     1261 gtagagtaac tgggaatcca ggggatgagg aattcattga accatccaca gaaaatgaat
     1321 ttggacttac gtctttgcgt tggcaaaatg attccccaac ctttggagaa catcagcttg
     1381 ccagcagctc tgaggtgcaa aatggaagtc ccatgtctca gactgagact gtgtctaggt
     1441 cagtcgcacc catgagaggt ggagagatca ctgcacactg gctcttgacc aacagcacaa
     1501 catctgcaga tgtgacagga agctctgctt catatcctga aggtgtgaat gcttcagtgt
     1561 tgacccagtt ctcagactct actgtacagt ctggaggaag tcacacagca ttgggagata
     1621 ggagttattc agagtcttca tctacatctt cctcggaaag cttgaattca tcagcaccac
     1681 gtggagaacg ttcgatcgct gggattagct acggtcaagt gcgtggcaca gctattgaac
     1741 aaaggacttc cagcgaccac acagaccaca cctacctgtc atctactttc accaaaggag
     1801 aacgggcgtt actgtccatt acagataaca gttcatcctc agacattgtg gagagctcaa
     1861 cttcttatat taaaatctca aactcttcac attcagagta ttcctccttt tttcatgctc
     1921 agactgagag aagtaacatc tcatcctatg acggggaata tgctcagcct tctactgagt
     1981 cgccagttct gcatacatcc aaccttccgt cctacacacc caccattaat atgccgaaca
     2041 cttcggttgt tctggacact gatgctgagt ttgttagtga ctcctcctcc tcctcttcct
     2101 cctcctcctc ttcttcttct tcagggcctc ctttgcctct gccctctgtg tcacaatccc
     2161 accatttatt ttcatcaatt ttaccatcaa ccagggcctc tgtgcatcta ctaaagtcta
     2221 cctctgatgc atccacacca tggtcttcct caccatcacc tttaccagta tccttaacga
     2281 catctacatc tgccccactt tctgtctcac aaacaacctt gccacagtca tcttctaccc
     2341 ctgtcctgcc cagggcaagg gagactcctg tgacttcatt tcagacatca acaatgacat
     2401 cattcatgac aatgctccat agtagtcaaa ctgcagacct taagagccag agcaccccac
     2461 accaagagaa agtcattaca gaatcaaagt caccaagcct ggtgtctctg cccacagagt
     2521 ccaccaaagc tgtaacaaca aactctcctt tgcctccatc cttaacagag tcctccacag
     2581 agcaaaccct tccagccaca agcaccaact tagcacaaat gtctccaact ttcacaacta
     2641 ccattctgaa gacctctcag cctcttatga ccactcctgg caccctgtca agcacagcat
     2701 ctctggtcac tggccctata gccgtacaga ctacagctgg aaaacagctc tcgctgaccc
     2761 atcctgaaat actagttcct caaatctcaa cagaaggtgg catcagcaca gaaaggaacc
     2821 gagtgattgt ggatgctacc actggattga tccctttgac cagtgtaccc acatcagcaa
     2881 aagaaatgac cacaaagctt ggcgttacag cagagtacag cccagcttca cgttccctcg
     2941 gaacatctcc ttctccccaa accacagttg tttccacggc tgaagacttg gctcccaaat
     3001 ctgccacctt tgctgttcag agcagcacac agtcaccaac aacagtgtcc tcttcagcct
     3061 cagtcaacag ctgtgctgtg aacccttgtc ttcacaatgg cgaatgcgtc gcagacaaca
     3121 ccagccgtgg ctaccactgc aggtgcccgc cttcctggca aggggatgat tgcagtgtgg
     3181 atgtgaatga gtgcctgtcg aacccctgcc catccacagc catgtgcaac aatactcagg
     3241 gatcctttat ctgcaaatgc ccggttgggt accagttgga aaaagggata tgcaatttgg
     3301 ttagaacctt cgtgacagag tttaaattaa agagaacttt tcttaataca actgtggaaa
     3361 aacattcaga cctacaagaa gttgaaaatg agatcaccaa aacgttaaat atgtgttttt
     3421 cagcgttacc tagttacatc cgatctacag ttcacgcctc tagggagtcc aacgcggtgg
     3481 tgatctcact gcaaacaacc ttttccctgg cctccaatgt gacgctattt gacctggctg
     3541 ataggatgca gaaatgtgtc aactcctgca agtcctctgc tgaggtctgc cagctcttgg
     3601 gatctcagag gcggatcttt agagcgggca gcttgtgcaa gcggaagagt cccgaatgtg
     3661 acaaagacac ctccatctgc actgacctgg acggcgttgc cctgtgccag tgcaagtcgg
     3721 gatactttca gttcaacaag atggaccact cctgccgagc atgtgaagat ggatataggc
     3781 ttgaaaatga aacctgcatg agttgcccat ttggccttgg tggtctcaac tgtggaaacc
     3841 cctatcagct tatcactgtg gtgatcgcag ccgcgggagg tgggctcctg ctcatcctag
     3901 gcatcgcact gattgttacc tgttgcagaa agaataaaaa tgacataagc aaactcatct
     3961 tcaaaagtgg agatttccaa atgtccccgt atgctgaata ccccaaaaat cctcgctcac
     4021 aagaatgggg ccgagaagct attgaaatgc atgagaatgg aagtaccaaa aacctcctcc
     4081 agatgacgga tgtgtactac tcgcctacaa gtgtaaggaa tccagaactt gaacgaaacg
     4141 gactctaccc ggcctacact ggactgccag gatcacggca ttcttgcatt ttccccggac
     4201 agtataaccc gtctttcatc agtgatgaaa gcagaagaag agactacttt taagtccagg
     4261 agagagaggg actcattgct ctgagccagt cacctgggac ctctgctcag aggaccgcac
     4321 caggaggctg cgcccaggat ttgtcgggag ccacgctgag tggcaagcag gaagagggac
     4381 aggcatgcgg ggcgtgacca cagtggagga gacaggtgga tgtggaacca caggctgctc
     4441 attcagcacc tttgttgtta ctgtgaacgt gaatgtgggc cagtatcaag agagtctctc
     4501 tgagtgactg caccatggca ctggcaccag ggcgactatt agccagggca gaccactaga
     4561 cttcagtgca gggacctggt tttcccttcg tttgcacttt agtaaattgg gtgggaggtt
     4621 tccttttgga tctgttttga gactgttcca gaaagaaggc ttcctttccc gagacacttc
     4681 cataggcagc aatttggtga ttcatttgca gcaaaatact ggcttgttaa ttattttcct
     4741 gcccagcgcc tgcgtgctaa acaacagatg aggatgagcg taccactgaa gtctgaagat
     4801 gtcgccattg aacggacagt gttttcatat gtttctaggt tgtcttatgc tacagtttcc
     4861 aagccagccc ccacagtgag gaaatgtgtg aggcaccgca cacaactgca atgtgttttt
     4921 taagtcaagg tgacacatgt atttaagatt ttttttttaa atctctttgc agttaaatct
     4981 cactttttca aacaagcctg gatcagggca aaacaactta tatttggttt tagctggagg
     5041 ctcagcaggc agattgcagg caggggggca cttttcatcc atgagggccc agcctggggc
     5101 ctgggactcg atcaccattg tggaggccag aggcagctgc gtatggagga gaaatgtcaa
     5161 actgaacgca ggtttcacca ctctaggaaa gcagcttgtt gagcccctgc agctggatgt
     5221 ggttagaggg atgggctgaa taggcaggtt agatttcctg catcaacagt gctttgggaa
     5281 gctgtgtgga ttcctgagga agaacaggga gccgagatgg agccacacat gagtttgctc
     5341 accggctact gcagcacttt gtacccagaa tctcatgtcc acaaacccca tgtaaacttt
     5401 caaccactca aagctgttta ttcggctgaa gaaataactt ttttttctca cccagtcatt
     5461 tgtacctctt catatggcta tgtcgcaccc tccagaaacg tggttatact tccagtcagt
     5521 gtgggagaac tgaagacttc cggttggtcg aggaactgag ggttgacctt cgggaaggaa
     5581 gttccactca tcttatttat tatgcctgtg atgtgggtcc tgccagggag acatccagta
     5641 ctcggtgtct ttaattgcca cctggggaac tgtgtttatt ggccttcttt ggggcatcct
     5701 ggttttggat gaagtgaggg gaatacagag gtaaaagaat tgtctccacc ctgaagcggg
     5761 gagtcccgct tcacatttct ggaaatggtg cagccactgg ggacagttct gccccgggca
     5821 tggttgtttc ttcaaggtcc tctaaatata atccctattc ttacataatc cttggccctg
     5881 atggttttaa gcaagaactc ctgtgtccca tggtctccac cactcaccat caccctgctg
     5941 tagcaagagt cctagtcagg ggaggtgcat tttagtagtt acattgcact tatccatgag
     6001 ataaataaaa ggagaactgt ttttatcagt ggaggctaac ctaaaatttc aaagtgtcgc
     6061 ctttttgaaa tcttgggcct ctctctctgt agaaccaatg gccctttgtg gctcacggcc
     6121 tcgcacctaa ctggagagtt ctgagctcct gcagctcacc tgagcccaca gactaggctt
     6181 cttggctcct tccgcagcat gcctgctcac ccccagaacc cgcagctgtg ggaagagcca
     6241 tgtagggagg ctattcccag gcatacactt ccactgcctt cagctgacgt cacagctgac
     6301 aaatcatctc ctctatcgga gccagaagac ttcagctcca caaaatgaag tgttctgtcc
     6361 tgaaaacatt cttgggaaga atcccaacat cgagaaaacg gtgtcctgtg agttccaaca
     6421 atgcttcttg ttcatgggtt tcttccgtat ggagtggatt aagagtgttt tattttgttg
     6481 ttctaactga gaaaaaaagg aggcacccac aaggttgagg tcacacagtc tccacagttt
     6541 ccaggaggcg tttgggggtg gggaaggcac ctccagagca tgaggctcta aggggacatg
     6601 agtaaagcat gtctgtgacc cagtgaggaa gggagaggcc agctgcactc ctgcacgggg
     6661 ttcctagctg cagaagggtc ccgcctaggc cgaggggaaa cacctgatag cagaagaggc
     6721 ctggatgcac acctggcacg ccgaggctct ccgcccagac acagtgctcc atgtcagccc
     6781 ctgcacctgg ggtgtgtgat tcacgtgcac agatgccaca atcctgcacc aatatcccac
     6841 agatggggga aggtgagagg aaggggcaag tgatgtgtaa ctgctcaaga gatgcttaaa
     6901 cctccataga gaggagccgg gcgcaggggc atctgtgtgt cccgtcacac actgcagcag
     6961 ggaagggtgg ctggctggct ccctggcatc agtggtttgg tttaagctcc agagggtctt
     7021 attgccattg tcttttcctc tgccccttga gccagcctaa ggccctggag tctgtttctt
     7081 taggcggatg aactgacatg ctcctaccat gaccaggctc tgggcaaggc tcctcacagt
     7141 atccttgaga ggtgggcatg gaagtgccca tttctcaggt acagaaacct tcagagagga
     7201 taaatagctt gccctgtaga agcaggactg aaacccttgt ctgcctgact cccccagcta
     7261 ctctgcccac tgtagccccc tgccttactg tcctggcaca cccctcacca tcctgtatac
     7321 cttaaatatc aaagagggca agagagaaag ggctttaaag ataagttatt tttttaagga
     7381 accttaatat tatttttaag aagtaaccaa attagtgacg tgaaatgcaa aaaaaaaaaa
     7441 aaaaaaatgc tgactaccct tttgaaaatg tgctttcaga ttgtttttta tatgtaattc
     7501 ttagacactt gtcattaaga aaatagtggc tggcttgtgc tcagcaagaa gcacactggc
     7561 acgtggcttt ggtataggaa gtggaaggca aggacctggg tttctgacaa gtgcagtcag
     7621 acatacacat ccatctgaga gctgcttgcc ttgttcccgt ggtaaggcca tggctcccac
     7681 atactggaag catatttttg tgaactgttt tcagaattgg gaacatattc agtggaatgt
     7741 cttaaacgct gacacatctt cctcctttga ggggaagtat attttcagaa gcagctaaga
     7801 catttagagc caaaattgct gataggtggt tcagtcaagt tggctgatgg catctgaggt
     7861 caaaaactaa gatgtgacga ttgagttctt aaagaggtaa ctcttaaagt gggcccaagg
     7921 gagaaggcag catctttgga ctcctaggct ggatggctac ccaagaatat gccattgaag
     7981 gatgacacct attcagatgt gaaagttccc agagcaggca cctttgggtc acccaccacg
     8041 tgtggctttt tggtgcctgc atgaaactgt gttgccccct ggtgagatgc ccgggtgtcc
     8101 tgtaagccga tttaactctg cctgaggaag aggagctgtc cactccagtt gcccttggct
     8161 aagtttagcc taacacacag ggttttgacc catagttcta aaatacacaa attttgagac
     8221 tacagcactt ctttggaaag aggaagaatg caaagttcag tatttcaata ctttgtattt
     8281 tacttgaaat tacccttagt agcatctttt ttttcctgtc tgaaagcttt tgtgtggatg
     8341 agaagggaca tttcatttcc tcccttaaca aagtgtcatt ctgaggttct catgtgtgtt
     8401 tttggaaata gagatactgg ttttgtagag tttgcctttg ggtatgtttt ctttttttct
     8461 taaatctcca aggaagagaa ctgactaaaa tagtaggaac atgaaagtat taaatgccaa
     8521 ttaatttgtt gtagtaaagt atcttcatta gcgttatact ccatcatatc tggtgtaaac
     8581 tgctcacaga aaaccctatg aaaccaaagg gggaccattc aggtctaaaa agcgacaggt
     8641 ccgagactgg gtctgtcacc tgggcatttt caaagaggac attttgaaga atttgcatat
     8701 tcagattttt aaaatgcact taacatactt cattacagat ttcttgggta gggaggatgg
     8761 gataggccag ggatgggatg gagtcagttc tgcctgggaa actaatccga atcatttacc
     8821 tttctgtatt aaccttggcc tgtcctaaaa agagaacgac tgtttcatca tgagttgctc
     8881 tgagttttgt taatgtttgt gttggtggat tgacggttaa atgaagcatt tagctggaat
     8941 atgaactttg ggagttttca tgttgtcctg gatttctctt tgtaaacctt taaaccttag
     9001 cccctggttg attgtgttaa acccattatg agaatgttat ttaaagttgt attataattg
     9061 caacctccac taattattca gtactgtagc agcaaagtat tatttgtaag aatttggtta
     9121 tttttatact tatatccact ctaagtctgg cgttctagtc agtaaaatga tgcaataaaa
     9181 ttaatatcat tttca
//
