LOCUS       NM_024602               3597 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens HECT domain E3 ubiquitin protein ligase 3 (HECTD3),
            mRNA.
ACCESSION   NM_024602 XM_371246
VERSION     NM_024602.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Huang,F., Huang,Z., Wei,Q., Liu,G. and Pu,J.
  TITLE     E3 ubiquitin ligase HECTD3 is a tumor suppressor and mediates the
            polyubiquitination of SLC7A11 to promote ferroptosis in colon
            cancer
  JOURNAL   Exp Cell Res 430 (1), 113697 (2023)
   PUBMED   37422058
  REMARK    GeneRIF: E3 ubiquitin ligase HECTD3 is a tumor suppressor and
            mediates the polyubiquitination of SLC7A11 to promote ferroptosis
            in colon cancer.
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   Zhang,G., Tan,R., Wan,S., Yang,R., Hu,X., Zhao,E., Ding,X.,
            Zhang,J., Li,B., Liang,P. and Cui,H.
  TITLE     HECTD3 regulates the tumourigenesis of glioblastoma by
            polyubiquitinating PARP1 and activating EGFR signalling pathway
  JOURNAL   Br J Cancer 127 (11), 1925-1938 (2022)
   PUBMED   36088509
  REMARK    GeneRIF: HECTD3 regulates the tumourigenesis of glioblastoma by
            polyubiquitinating PARP1 and activating EGFR signalling pathway.
REFERENCE   3  (bases 1 to 3597)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   4  (bases 1 to 3597)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 3597)
  AUTHORS   Jiang,Q., Li,F., Cheng,Z., Kong,Y. and Chen,C.
  TITLE     The role of E3 ubiquitin ligase HECTD3 in cancer and beyond
  JOURNAL   Cell Mol Life Sci 77 (8), 1483-1495 (2020)
   PUBMED   31637449
  REMARK    GeneRIF: This review describes the progress in the recent studies
            of HECTD3 in cancer and other diseases. [review]
            Review article
REFERENCE   6  (bases 1 to 3597)
  AUTHORS   Li,Z., Zhou,L., Prodromou,C., Savic,V. and Pearl,L.H.
  TITLE     HECTD3 Mediates an HSP90-Dependent Degradation Pathway for Protein
            Kinase Clients
  JOURNAL   Cell Rep 19 (12), 2515-2528 (2017)
   PUBMED   28636940
  REMARK    GeneRIF: HECTD3 as a tumor suppressor modulating the activity of
            this important oncogenic signaling pathway.
REFERENCE   7  (bases 1 to 3597)
  AUTHORS   Li,Y., Chen,X., Wang,Z., Zhao,D., Chen,H., Chen,W., Zhou,Z.,
            Zhang,J., Zhang,J., Li,H. and Chen,C.
  TITLE     The HECTD3 E3 ubiquitin ligase suppresses cisplatin-induced
            apoptosis via stabilizing MALT1
  JOURNAL   Neoplasia 15 (1), 39-48 (2013)
   PUBMED   23358872
  REMARK    GeneRIF: Overexpression of MALT1 partially rescues HECTD3
            depletion-induced apoptosis.
REFERENCE   8  (bases 1 to 3597)
  AUTHORS   Yu,J., Lan,J., Zhu,Y., Li,X., Lai,X., Xue,Y., Jin,C. and Huang,H.
  TITLE     The E3 ubiquitin ligase HECTD3 regulates ubiquitination and
            degradation of Tara
  JOURNAL   Biochem Biophys Res Commun 367 (4), 805-812 (2008)
   PUBMED   18194665
  REMARK    GeneRIF: All these findings suggest that HECTD3 may facilitate cell
            cycle progression via regulating ubiquitination and degradation of
            Tara.
REFERENCE   9  (bases 1 to 3597)
  AUTHORS   Gevaert,K., Goethals,M., Martens,L., Van Damme,J., Staes,A.,
            Thomas,G.R. and Vandekerckhove,J.
  TITLE     Exploring proteomes and analyzing protein processing by mass
            spectrometric identification of sorted N-terminal peptides
  JOURNAL   Nat Biotechnol 21 (5), 566-569 (2003)
   PUBMED   12665801
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA332198.1, AK096462.1,
            AK056545.1, AK056873.1 and AL359473.22.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_024602.5.
            
            Summary: The protein encoded by this gene transfers ubiquitin from
            an E2 ubiquitin-conjugating enzyme to targeted substrates, leading
            to the degradation of those substrates. The encoded protein has
            been shown to transfer ubiquitin to TRIOBP to facilitate cell cycle
            progression, and to STX8. [provided by RefSeq, Dec 2012].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853558.984.1,
                                           SRR11853559.1198.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000372172.5/ ENSP00000361245.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-333               DA332198.1         33-365
            334-793             AK096462.1         334-793
            794-2436            AK056545.1         176-1818
            2437-3185           AK056873.1         1362-2110
            3186-3597           AL359473.22        8370-8781           c
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p34.1"
     gene            1..3597
                     /gene="HECTD3"
                     /note="HECT domain E3 ubiquitin protein ligase 3"
                     /db_xref="GeneID:79654"
                     /db_xref="HGNC:HGNC:26117"
                     /db_xref="MIM:618638"
     exon            1..436
                     /gene="HECTD3"
                     /inference="alignment:Splign:2.1.0"
     CDS             68..2653
                     /gene="HECTD3"
                     /EC_number="2.3.2.26"
                     /note="HECT domain containing E3 ubiquitin protein ligase
                     3; HECT-type E3 ubiquitin transferase HECTD3"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase HECTD3"
                     /protein_id="NP_078878.3"
                     /db_xref="CCDS:CCDS41318.1"
                     /db_xref="GeneID:79654"
                     /db_xref="HGNC:HGNC:26117"
                     /db_xref="MIM:618638"
                     /translation="MAGPGPGAVLESPRQLLGRVRFLAEAARSLRAGRPLPAALAFVP
                     REVLYKLYKDPAGPSRVLLPVWEAEGLGLRVGAAGPAPGTGSGPLRAARDSIELRRGA
                     CVRTTGEELCNGHGLWVKLTKEQLAEHLGDCGLQEGWLLVCRPAEGGARLVPIDTPNH
                     LQRQQQLFGVDYRPVLRWEQVVDLTYSHRLGSRPQPAEAYAEAVQRLLYVPPTWTYEC
                     DEDLIHFLYDHLGKEDENLGSVKQYVESIDVSSYTEEFNVSCLTDSNADTYWESDGSQ
                     CQHWVRLTMKKGTIVKKLLLTVDTTDDNFMPKRVVVYGGEGDNLKKLSDVSIDETLIG
                     DVCVLEDMTVHLPIIEIRIVECRDDGIDVRLRGVKIKSSRQRELGLNADLFQPTSLVR
                     YPRLEGTDPEVLYRRAVLLQRFIKILDSVLHHLVPAWDHTLGTFSEIKQVKQFLLLSR
                     QRPGLVAQCLRDSESSKPSFMPRLYINRRLAMEHRACPSRDPACKNAVFTQVYEGLKP
                     SDKYEKPLDYRWPMRYDQWWECKFIAEGIIDQGGGFRDSLADMSEELCPSSADTPVPL
                     PFFVRTANQGNGTGEARDMYVPNPSCRDFAKYEWIGQLMGAALRGKEFLVLALPGFVW
                     KQLSGEEVSWSKDFPAVDSVLVKLLEVMEGMDKETFEFKFGKELTFTTVLSDQQVVEL
                     IPGGAGIVVGYGDRSRFIQLVQKARLEESKEQVAAMQAGLLKVVPQAVLDLLTWQELE
                     KKVCGDPEVTVDALRKLTRFEDFEPSDSRVQYFWEALNNFTNEDRSRFLRFVTGRSRL
                     PARIYIYPDKLGYETTDALPESSTCSSTLFLPHYASAKVCEEKLRYAAYNCVAIDTDM
                     SPWEE"
     exon            437..597
                     /gene="HECTD3"
                     /inference="alignment:Splign:2.1.0"
     exon            598..690
                     /gene="HECTD3"
                     /inference="alignment:Splign:2.1.0"
     exon            691..826
                     /gene="HECTD3"
                     /inference="alignment:Splign:2.1.0"
     exon            827..942
                     /gene="HECTD3"
                     /inference="alignment:Splign:2.1.0"
     exon            943..1056
                     /gene="HECTD3"
                     /inference="alignment:Splign:2.1.0"
     exon            1057..1139
                     /gene="HECTD3"
                     /inference="alignment:Splign:2.1.0"
     exon            1140..1305
                     /gene="HECTD3"
                     /inference="alignment:Splign:2.1.0"
     exon            1306..1387
                     /gene="HECTD3"
                     /inference="alignment:Splign:2.1.0"
     exon            1388..1570
                     /gene="HECTD3"
                     /inference="alignment:Splign:2.1.0"
     exon            1571..1623
                     /gene="HECTD3"
                     /inference="alignment:Splign:2.1.0"
     exon            1624..1688
                     /gene="HECTD3"
                     /inference="alignment:Splign:2.1.0"
     exon            1689..1792
                     /gene="HECTD3"
                     /inference="alignment:Splign:2.1.0"
     exon            1793..1912
                     /gene="HECTD3"
                     /inference="alignment:Splign:2.1.0"
     exon            1913..2002
                     /gene="HECTD3"
                     /inference="alignment:Splign:2.1.0"
     exon            2003..2209
                     /gene="HECTD3"
                     /inference="alignment:Splign:2.1.0"
     exon            2210..2339
                     /gene="HECTD3"
                     /inference="alignment:Splign:2.1.0"
     exon            2340..2414
                     /gene="HECTD3"
                     /inference="alignment:Splign:2.1.0"
     exon            2415..2496
                     /gene="HECTD3"
                     /inference="alignment:Splign:2.1.0"
     exon            2497..2568
                     /gene="HECTD3"
                     /inference="alignment:Splign:2.1.0"
     exon            2569..3597
                     /gene="HECTD3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3580..3585
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HECTD3"
                     /note="hexamer: AATAAA"
     polyA_site      3597
                     /gene="HECTD3"
                     /note="major polyA site"
ORIGIN      
        1 gtccgcggtc gcgccagctg ttccccgggc agggtcgcct ctaggtgctc acctccgcca
       61 cttcgccatg gcgggtcctg gcccgggcgc ggtgctggag tccccccggc agctgctggg
      121 ccgcgtgcgc ttcttggcag aggcagcgcg gagcctccgc gccgggcggc cgctgccagc
      181 agcgctggct ttcgtgccgc gagaggtgct ctacaagctt tacaaggacc cagcgggacc
      241 gtcgcgcgtg cttctgccgg tgtgggaggc agagggcctg gggctgcgtg tgggcgccgc
      301 aggcccagcc cccggtaccg gctccgggcc cctccgcgcc gcccgcgaca gcattgagct
      361 ccggcgcggc gcctgcgtgc gcaccacggg cgaggagctg tgcaatggcc acgggctctg
      421 ggtgaagctg acaaaggagc agctggcaga gcacctgggc gactgcgggc tgcaggaagg
      481 ctggctgctg gtgtgccgcc cggcggaggg cggagcccgc ctggtaccca tcgacactcc
      541 caaccacctc cagcggcagc agcagctctt tggcgtggat tatcggccgg tgctcaggtg
      601 ggaacaggtg gtggacctga catactcaca tcgcctggga tcgagacctc agccggcaga
      661 ggcatacgca gaagctgtac aaaggctact ctatgtaccc ccgacatgga cctacgagtg
      721 cgacgaggac ctgatccact tcttgtatga ccacctgggc aaggaggatg agaacctggg
      781 tagcgtgaag cagtatgtgg agagcataga cgtttcctcc tacacggagg agttcaacgt
      841 gtcctgcctg acagacagca atgccgatac ctactgggag agcgatgggt cccagtgcca
      901 acactgggta cggcttacta tgaagaaggg caccattgtc aagaagctgc tactcacagt
      961 ggataccaca gatgacaact ttatgccaaa gcgggtggtg gtctatgggg gtgaagggga
     1021 caacctgaag aagctgagtg acgtgagcat tgacgagacc ctcatcgggg atgtctgtgt
     1081 cctggaggac atgaccgtcc acctcccgat catcgagatc cgcatcgtgg agtgccgaga
     1141 tgatgggatt gatgttcgtc tccgaggggt caagatcaag tcatctagac agcgggaact
     1201 agggttgaat gcagacctgt tccagccaac tagtctggtg cgatatccac gcctagaagg
     1261 caccgaccct gaagtactgt accgcagagc tgtcctcctg cagagattca tcaagatcct
     1321 cgatagtgtc ctgcaccacc tggtacctgc ctgggaccac acactgggca ccttcagtga
     1381 gattaagcaa gtgaagcagt tcctactgct gtcccgccag cggccaggcc tggtggctca
     1441 gtgcctgcgt gactctgaga gcagcaagcc cagcttcatg ccacgcctat acatcaaccg
     1501 ccgtcttgcc atggaacacc gtgcctgccc ctctcgagac cctgcctgca agaatgcagt
     1561 cttcacccag gtatatgaag gcctcaagcc ctctgacaaa tatgaaaagc ccctggacta
     1621 caggtggccc atgcgctatg accagtggtg ggagtgtaaa tttattgcag aaggcatcat
     1681 tgaccaaggg ggtggtttcc gggacagcct ggcagatatg tcagaagagc tgtgccctag
     1741 ctcagcggat acccccgtgc ccctgccctt ctttgtacgc acagccaacc agggcaatgg
     1801 cactggtgag gctcgggaca tgtatgtacc caacccctcc tgccgagact ttgccaagta
     1861 tgaatggatc ggacagctga tgggggctgc ccttcggggt aaggagttcc tggtcctggc
     1921 cctgcctggt tttgtgtgga agcagctttc tggtgaggag gtgagctgga gcaaggactt
     1981 cccagctgtg gactctgtgc tggtgaagct cctggaagtg atggaaggaa tggacaagga
     2041 gacgtttgag ttcaagtttg ggaaggaact aacattcacc actgtactga gtgaccaaca
     2101 ggtggtggag ctgatccctg ggggtgcagg catcgtcgtg ggatatgggg accgttctcg
     2161 tttcatccaa ctggtccaga aggcacggct agaggagagc aaggagcagg tggcagctat
     2221 gcaggcaggt ctgctgaagg tggtaccaca ggctgtgctg gacttgctga cctggcaaga
     2281 gttggagaag aaagtgtgtg gggatccaga ggtcactgtg gatgctctgc gcaagctcac
     2341 ccggtttgag gacttcgagc catctgactc gcgggtgcag tatttctggg aggcactgaa
     2401 caacttcacc aacgaggacc ggagccgctt cctgcgcttt gtcacgggcc gcagtcgcct
     2461 gccagcacgg atctacatct acccagacaa gctgggctac gagaccacag acgcgctgcc
     2521 cgagtcttcc acttgctcca gcaccctctt cctgccacac tatgccagtg ccaaggtatg
     2581 cgaggagaag ctccgctatg cggcctacaa ctgcgtggcc atcgacactg acatgagccc
     2641 ttgggaggag tgaggcgtgc cgccggctgt gggaccagca agactgcacg tgtccctctt
     2701 ggccttgccc agggcgaaga caccttccct gccctggttt ggctgacgtg ctcagcaaaa
     2761 ccccatgtgc cctgctcctg tgtgcagttg gggtaggggc agctggcatg gtcaggtaac
     2821 actagtggcc cagccccgca gacccacaag ccctacccgt gctggggctt gcttcccgag
     2881 gtatttcacc tcttaagagg gaatcttcca caagcccagc acaagctgcc aggcctgagc
     2941 tacttgaagg gggccatcta ggtccccaac ccatggactt tgcctccatt ttcagctccg
     3001 ccttttttct cctattttct ctctggcttt cttcagccat gactcacaac taaaaacata
     3061 aaacactgga ggttagtgga ggcccctccc caagcaggga gcctgggatg ggcagggagt
     3121 gatagccaaa ctccttggtc acctgctcca agaaggaagc agtagctgag cacctgccct
     3181 cacattctgc tcttttcccc tctccctcca taccagagat gtggtgagct ctgttcttct
     3241 accaacccag tctcaacaca caaagtgcca ccaccttccc tgactcagaa cccacatcca
     3301 ctcaatgtga actctactac cacgacctcc ccatattcct cacttctcca tcacctccag
     3361 cctgactccc tgtctgccct ttcaccccca agattttgca caggttaagg ccagttatgg
     3421 cctttttgaa atctgtaata gctccccttt ccccaactct aaagcctaga ccttaaacct
     3481 gttcctagag ctatgcacac ccctgcccca gtttaccgtt cctccctcag ggcctccgtg
     3541 acactccatg aaaagaagtt cttgcatacc ggaaagttga ataaatggat gaattca
//
