LOCUS       NM_182765               4862 bp    mRNA    linear   PRI 28-AUG-2024
DEFINITION  Homo sapiens HECT domain E3 ubiquitin protein ligase 2 (HECTD2),
            transcript variant 1, mRNA.
ACCESSION   NM_182765
VERSION     NM_182765.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Lv,D., Chen,Y., Tang,L., Tian,Y., Ren,D., Jian,N. and Shen,T.
  TITLE     HECTD2/TNFAIP1 Axis Regulating the p38/JNK Pathway to Promote an
            Inflammatory Response in Renal Cell Carcinoma Cells
  JOURNAL   In Vivo 38 (3), 1094-1103 (2024)
   PUBMED   38688591
  REMARK    GeneRIF: HECTD2/TNFAIP1 Axis Regulating the p38/JNK Pathway to
            Promote an Inflammatory Response in Renal Cell Carcinoma Cells.
REFERENCE   2  (bases 1 to 4862)
  AUTHORS   Ryu,T.Y., Kim,K., Han,T.S., Lee,M.O., Lee,J., Choi,J., Jung,K.B.,
            Jeong,E.J., An,D.M., Jung,C.R., Lim,J.H., Jung,J., Park,K.,
            Lee,M.S., Kim,M.Y., Oh,S.J., Hur,K., Hamamoto,R., Park,D.S.,
            Kim,D.S., Son,M.Y. and Cho,H.S.
  TITLE     Human gut-microbiome-derived propionate coordinates proteasomal
            degradation via HECTD2 upregulation to target EHMT2 in colorectal
            cancer
  JOURNAL   ISME J 16 (5), 1205-1221 (2022)
   PUBMED   34972816
  REMARK    GeneRIF: Human gut-microbiome-derived propionate coordinates
            proteasomal degradation via HECTD2 upregulation to target EHMT2 in
            colorectal cancer.
REFERENCE   3  (bases 1 to 4862)
  AUTHORS   Ottina,E., Panova,V., Doglio,L., Kazachenka,A., Cornish,G.,
            Kirkpatrick,J., Attig,J., Young,G.R., Litchfield,K., Lesluyes,T.,
            Van Loo,P., Swanton,C., MacRae,J., Tuting,T. and Kassiotis,G.
  TITLE     E3 ubiquitin ligase HECTD2 mediates melanoma progression and immune
            evasion
  JOURNAL   Oncogene 40 (37), 5567-5578 (2021)
   PUBMED   34145398
  REMARK    GeneRIF: E3 ubiquitin ligase HECTD2 mediates melanoma progression
            and immune evasion.
REFERENCE   4  (bases 1 to 4862)
  AUTHORS   Sun,T., Wang,X., He,H.H., Sweeney,C.J., Liu,S.X., Brown,M.,
            Balk,S., Lee,G.S. and Kantoff,P.W.
  TITLE     MiR-221 promotes the development of androgen independence in
            prostate cancer cells via downregulation of HECTD2 and RAB1A
  JOURNAL   Oncogene 33 (21), 2790-2800 (2014)
   PUBMED   23770851
  REMARK    GeneRIF: A major biological consequence of upregulation of miR-221
            is reprogramming of androgen receptor signaling via downregulation
            of HECTD2 and RAB1A.
REFERENCE   5  (bases 1 to 4862)
  AUTHORS   Jeong,B.H., Lee,K.H., Lee,Y.J., Yun,J., Park,Y.J., Cho,H.J.,
            Kim,Y.H., Cho,Y.S., Choi,E.K., Carp,R.I. and Kim,Y.S.
  TITLE     Absence of association between two HECTD2 polymorphisms and
            sporadic Creutzfeldt-Jakob disease
  JOURNAL   Dement Geriatr Cogn Disord 31 (2), 146-151 (2011)
   PUBMED   21335971
  REMARK    GeneRIF: HECTD2 polymorphisms are not associated with genetic
            susceptibility to sporadic Creutzfeldt-Jakob disease in a Korean
            population
REFERENCE   6  (bases 1 to 4862)
  AUTHORS   Ebstein,F., Lange,N., Urban,S., Seifert,U., Kruger,E. and
            Kloetzel,P.M.
  TITLE     Maturation of human dendritic cells is accompanied by functional
            remodelling of the ubiquitin-proteasome system
  JOURNAL   Int J Biochem Cell Biol 41 (5), 1205-1215 (2009)
   PUBMED   19028597
REFERENCE   7  (bases 1 to 4862)
  AUTHORS   Grupe,A., Li,Y., Rowland,C., Nowotny,P., Hinrichs,A.L., Smemo,S.,
            Kauwe,J.S., Maxwell,T.J., Cherny,S., Doil,L., Tacey,K., van
            Luchene,R., Myers,A., Wavrant-De Vrieze,F., Kaleem,M.,
            Hollingworth,P., Jehu,L., Foy,C., Archer,N., Hamilton,G.,
            Holmans,P., Morris,C.M., Catanese,J., Sninsky,J., White,T.J.,
            Powell,J., Hardy,J., O'Donovan,M., Lovestone,S., Jones,L.,
            Morris,J.C., Thal,L., Owen,M., Williams,J. and Goate,A.
  TITLE     A scan of chromosome 10 identifies a novel locus showing strong
            association with late-onset Alzheimer disease
  JOURNAL   Am J Hum Genet 78 (1), 78-88 (2006)
   PUBMED   16385451
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   8  (bases 1 to 4862)
  AUTHORS   Deloukas,P., Earthrowl,M.E., Grafham,D.V., Rubenfield,M.,
            French,L., Steward,C.A., Sims,S.K., Jones,M.C., Searle,S.,
            Scott,C., Howe,K., Hunt,S.E., Andrews,T.D., Gilbert,J.G.,
            Swarbreck,D., Ashurst,J.L., Taylor,A., Battles,J., Bird,C.P.,
            Ainscough,R., Almeida,J.P., Ashwell,R.I., Ambrose,K.D.,
            Babbage,A.K., Bagguley,C.L., Bailey,J., Banerjee,R., Bates,K.,
            Beasley,H., Bray-Allen,S., Brown,A.J., Brown,J.Y., Burford,D.C.,
            Burrill,W., Burton,J., Cahill,P., Camire,D., Carter,N.P.,
            Chapman,J.C., Clark,S.Y., Clarke,G., Clee,C.M., Clegg,S., Corby,N.,
            Coulson,A., Dhami,P., Dutta,I., Dunn,M., Faulkner,L., Frankish,A.,
            Frankland,J.A., Garner,P., Garnett,J., Gribble,S., Griffiths,C.,
            Grocock,R., Gustafson,E., Hammond,S., Harley,J.L., Hart,E.,
            Heath,P.D., Ho,T.P., Hopkins,B., Horne,J., Howden,P.J., Huckle,E.,
            Hynds,C., Johnson,C., Johnson,D., Kana,A., Kay,M., Kimberley,A.M.,
            Kershaw,J.K., Kokkinaki,M., Laird,G.K., Lawlor,S., Lee,H.M.,
            Leongamornlert,D.A., Laird,G., Lloyd,C., Lloyd,D.M., Loveland,J.,
            Lovell,J., McLaren,S., McLay,K.E., McMurray,A.,
            Mashreghi-Mohammadi,M., Matthews,L., Milne,S., Nickerson,T.,
            Nguyen,M., Overton-Larty,E., Palmer,S.A., Pearce,A.V., Peck,A.I.,
            Pelan,S., Phillimore,B., Porter,K., Rice,C.M., Rogosin,A.,
            Ross,M.T., Sarafidou,T., Sehra,H.K., Shownkeen,R., Skuce,C.D.,
            Smith,M., Standring,L., Sycamore,N., Tester,J., Thorpe,A.,
            Torcasso,W., Tracey,A., Tromans,A., Tsolas,J., Wall,M., Walsh,J.,
            Wang,H., Weinstock,K., West,A.P., Willey,D.L., Whitehead,S.L.,
            Wilming,L., Wray,P.W., Young,L., Chen,Y., Lovering,R.C.,
            Moschonas,N.K., Siebert,R., Fechtel,K., Bentley,D., Durbin,R.,
            Hubbard,T., Doucette-Stamm,L., Beck,S., Smith,D.R. and Rogers,J.
  TITLE     The DNA sequence and comparative analysis of human chromosome 10
  JOURNAL   Nature 429 (6990), 375-381 (2004)
   PUBMED   15164054
REFERENCE   9  (bases 1 to 4862)
  AUTHORS   Kumar,S., Connor,J.R., Dodds,R.A., Halsey,W., Van Horn,M., Mao,J.,
            Sathe,G., Mui,P., Agarwal,P., Badger,A.M., Lee,J.C., Gowen,M. and
            Lark,M.W.
  TITLE     Identification and initial characterization of 5000 expressed
            sequenced tags (ESTs) each from adult human normal and
            osteoarthritic cartilage cDNA libraries
  JOURNAL   Osteoarthritis Cartilage 9 (7), 641-653 (2001)
   PUBMED   11597177
REFERENCE   10 (bases 1 to 4862)
  AUTHORS   Robertson,N.G., Khetarpal,U., Gutierrez-Espeleta,G.A., Bieber,F.R.
            and Morton,C.C.
  TITLE     Isolation of novel and known genes from a human fetal cochlear cDNA
            library using subtractive hybridization and differential screening
  JOURNAL   Genomics 23 (1), 42-50 (1994)
   PUBMED   7829101
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC023902.7 and AL161798.23.
            
            On May 21, 2020 this sequence version replaced NM_182765.5.
            
            Transcript Variant: This variant (1) lacks an alternate in-frame
            exon in the 5' coding region compared to variant 3. It encodes
            isoform a which is shorter compared to isoform c.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC040187.1, SRR1660807.136853.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968189, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000298068.10/ ENSP00000298068.5
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-233               AC023902.7         165028-165260
            234-363             AC023902.7         179965-180094
            364-502             AL161798.23        29621-29759
            503-605             AL161798.23        30448-30550
            606-695             AL161798.23        31289-31378
            696-760             AL161798.23        47395-47459
            761-806             AL161798.23        50288-50333
            807-916             AL161798.23        52161-52270
            917-1065            AL161798.23        53701-53849
            1066-1189           AL161798.23        54374-54497
            1190-1286           AL161798.23        56876-56972
            1287-1394           AL161798.23        60394-60501
            1395-1527           AL161798.23        61546-61678
            1528-1616           AL161798.23        62614-62702
            1617-1775           AL161798.23        65408-65566
            1776-1850           AL161798.23        67302-67376
            1851-1938           AL161798.23        68066-68153
            1939-2045           AL161798.23        68238-68344
            2046-2161           AL161798.23        69696-69811
            2162-2305           AL161798.23        70385-70528
            2306-4862           AL161798.23        81458-84014
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q23.32"
     gene            1..4862
                     /gene="HECTD2"
                     /note="HECT domain E3 ubiquitin protein ligase 2"
                     /db_xref="GeneID:143279"
                     /db_xref="HGNC:HGNC:26736"
                     /db_xref="MIM:620876"
     exon            1..233
                     /gene="HECTD2"
                     /inference="alignment:Splign:2.1.0"
     CDS             96..2426
                     /gene="HECTD2"
                     /EC_number="2.3.2.26"
                     /note="isoform a is encoded by transcript variant 1;
                     probable E3 ubiquitin-protein ligase HECTD2; HECT
                     domain-containing protein 2; HECT domain containing E3
                     ubiquitin protein ligase 2; HECT-type E3 ubiquitin
                     transferase HECTD2"
                     /codon_start=1
                     /product="probable E3 ubiquitin-protein ligase HECTD2
                     isoform a"
                     /protein_id="NP_877497.4"
                     /db_xref="CCDS:CCDS7414.1"
                     /db_xref="GeneID:143279"
                     /db_xref="HGNC:HGNC:26736"
                     /db_xref="MIM:620876"
                     /translation="MSEAVRVPSPATPLVVAAPAPEERKGKESEREKLPPIVSAGAGA
                     TAGLDRGAKGQISTFSSFISAVSPKKEAAENRSSPAHLVFPNIKNVREPPPICLDVRQ
                     KQRTSMDASSSEMKAPVLPEPILPIQPKTVKDFQEDVEKVKSSGDWKAVHDFYLTTFD
                     SFPELNAAFKKDATASFNTIEDSGINAKFVNAVYDTLLNTPQDVQKTVLKGIINSLLR
                     EWKGPRTKDDLRAYFILLQNPQFNNTSTYVIYAHLLRQIATLVEADHHFLVHWFKKLS
                     QKRFKQLVERLLQFISLRLFPAKPEEFPPITKCSWWIPSAAKVLALLNTANNLVHPPL
                     IPYTDFYNSTLDHIDLMEEYHTWQNFGNSHRFSFCQYPFVISVAAKKIIIQRDSEQQM
                     INIARQSLVDKVSRRQRPDMNILFLNMKVRRTHLVSDSLDELTRKRADLKKKLKVTFV
                     GEAGLDMGGLTKEWFLLLIRQIFHPDYGMFTYHKDSHCHWFSSFKCDNYSEFRLVGIL
                     MGLAVYNSITLDIRFPPCCYKKLLSPPIIPSDQNIPVGICNVTVDDLCQIMPELAHGL
                     SELLSHEGNVEEDFYSTFQVFQEEFGIIKSYNLKPGGDKISVTNQNRKEYVQLYTDFL
                     LNKSIYKQFAAFYYGFHSVCASNALMLLRPEEVEILVCGSPDLDMHALQRSTQYDGYA
                     KTDLTIKYFWDVVLGFPLDLQKKLLHFTTGSDRVPVGGMADLNFKISKNETSTNCLPV
                     AHTCFNQLCLPPYKSKKDLKQKLIIGISNSEGFGLE"
     exon            234..363
                     /gene="HECTD2"
                     /inference="alignment:Splign:2.1.0"
     exon            364..502
                     /gene="HECTD2"
                     /inference="alignment:Splign:2.1.0"
     exon            503..605
                     /gene="HECTD2"
                     /inference="alignment:Splign:2.1.0"
     exon            606..695
                     /gene="HECTD2"
                     /inference="alignment:Splign:2.1.0"
     exon            696..760
                     /gene="HECTD2"
                     /inference="alignment:Splign:2.1.0"
     exon            761..806
                     /gene="HECTD2"
                     /inference="alignment:Splign:2.1.0"
     exon            807..916
                     /gene="HECTD2"
                     /inference="alignment:Splign:2.1.0"
     exon            917..1065
                     /gene="HECTD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1066..1189
                     /gene="HECTD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1190..1286
                     /gene="HECTD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1287..1394
                     /gene="HECTD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1395..1527
                     /gene="HECTD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1528..1616
                     /gene="HECTD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1617..1775
                     /gene="HECTD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1776..1850
                     /gene="HECTD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1851..1938
                     /gene="HECTD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1939..2045
                     /gene="HECTD2"
                     /inference="alignment:Splign:2.1.0"
     exon            2046..2161
                     /gene="HECTD2"
                     /inference="alignment:Splign:2.1.0"
     exon            2162..2305
                     /gene="HECTD2"
                     /inference="alignment:Splign:2.1.0"
     exon            2306..4862
                     /gene="HECTD2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4783..4788
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HECTD2"
                     /note="hexamer: ATTAAA"
     polyA_site      4800
                     /gene="HECTD2"
                     /note="major polyA site"
     regulatory      4840..4845
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HECTD2"
                     /note="hexamer: ATTAAA"
     polyA_site      4862
                     /gene="HECTD2"
ORIGIN      
        1 agagccctct cgcggccgcg gcggcagcag cagcgccagc cccagcaaca ctgaggccgc
       61 cgccgccgcc tggcgctccc gccgcccggc ccgacatgag tgaggcggtt cgggtaccct
      121 cgcccgccac tccgctggtg gtggcggcgc ccgcgcctga ggagaggaaa gggaaggagt
      181 cagagcgcga gaagctgccg cccatcgtat cggcgggcgc cggcgcgacc gcgggtttgg
      241 acagaggagc caaaggccaa atttccactt tcagcagttt tatttcagct gttagcccga
      301 agaaagaagc tgctgaaaac agaagttcac ctgcacatct tgttttccct aacatcaaga
      361 atgtgagaga accaccacca atttgccttg atgttagaca aaaacagcgt acatctatgg
      421 atgcatcatc atccgaaatg aaggccccag tccttccaga acctattctt cctatccagc
      481 ccaaaactgt gaaagacttt caggaagatg tagaaaaagt taagtcatca ggagattgga
      541 aagcagtaca tgatttttat ctaacaacgt ttgattcttt cccagaatta aatgctgcat
      601 ttaagaaaga tgccactgcc tcatttaaca ccattgaaga ctctgggatt aatgctaaat
      661 ttgtgaatgc tgtgtatgat accttactta atactcctca agacgttcag aagacagtat
      721 taaagggaat cattaacagc ctgttacgag aatggaaagg tccacgaaca aaagatgatc
      781 ttagagcata ttttatactt ttacagaatc ctcagtttaa taacacatct acgtatgtca
      841 tctatgctca cttgctacga cagatagcta ccttagtgga agctgaccat catttcctag
      901 ttcactggtt taaaaaattg tcccagaaga ggttcaaaca attggtagag agattgctgc
      961 aatttatttc tttacgcctg tttcctgcaa agcctgaaga atttccacct ataacaaagt
     1021 gttcctggtg gattccatca gccgctaaag tgttggcttt acttaatact gcaaacaatt
     1081 tagttcaccc tccccttatt ccttatactg atttctataa ttctacattg gatcacattg
     1141 atctcatgga agaatatcat acttggcaaa actttggaaa ctctcacagg ttttccttct
     1201 gtcagtaccc attcgttatt tctgtagctg caaaaaaaat cattattcag agagactcag
     1261 agcaacagat gataaacatc gcaaggcaaa gtctggtgga taaagtatct cgaagacaga
     1321 gacctgatat gaatatatta tttctaaata tgaaagtaag acggacacat ctggttagcg
     1381 attcacttga tgagctaacc cggaaaagag ccgatttgaa aaagaagtta aaagttacat
     1441 ttgtagggga agctggtttg gatatgggtg gtttgactaa agaatggttc cttcttctaa
     1501 ttcgccaaat ttttcatcca gattatggca tgtttacata tcacaaggat tcacactgcc
     1561 attggtttag cagctttaaa tgtgataact attctgaatt ccgattggtt ggaattctta
     1621 tgggactagc tgtttataac agcatcacct tggatattcg tttccctccc tgctgttaca
     1681 agaaattatt gagccctccc atcattccta gtgatcaaaa tataccagta ggcatctgca
     1741 atgttaccgt ggacgactta tgtcaaatta tgcctgagtt ggcccatgga ttaagtgaac
     1801 tcttatcaca tgaaggcaat gttgaagaag atttctattc aacatttcag gtttttcaag
     1861 aagaatttgg aataatcaag tcctataatt taaagcccgg tggtgataaa atttcagtta
     1921 ccaatcaaaa tagaaaagaa tatgtacagc tttatactga cttccttctg aacaaatcca
     1981 tctataagca gtttgctgca ttttattatg gatttcatag tgtgtgtgct tcaaatgccc
     2041 taatgctgct tcgtccagaa gaggttgaaa ttttggtctg tggaagtcct gacctggata
     2101 tgcatgctct gcagagaagt actcagtatg atggctatgc aaaaacggac ttaactataa
     2161 aatacttttg ggatgttgtg cttggatttc ctcttgatct tcaaaagaag ttgctacatt
     2221 ttactacagg gagtgacaga gtacctgtag gagggatggc tgatttgaac tttaaaatct
     2281 caaagaatga aacttctact aactgcttac ctgtggccca tacctgcttc aatcaacttt
     2341 gccttccccc ctacaagagc aaaaaggatc tgaaacagaa attgatcatt ggaatttcaa
     2401 attcagaagg ttttggactt gagtaaccta gaagacttga aatataatct tttatatgta
     2461 gcattcactt ccctcttact gtgcctttag ccttttcatg tttctgtctc aaaacacttt
     2521 gacaaagctc accaacttta aaatattaag tttttaaaaa atcaaatatg aagtatgttc
     2581 agcaaaggca taattttcta aaaacacaca cagaaatcgc ttgagtgagg aaaagtccac
     2641 atggcagaga caggtatggt ccagttcctc ttttatatag cactttatca ttctccacaa
     2701 gtagtttgtc acaggcattc cactgggaaa gcaaagtaca gtgaagaatg aatttatggt
     2761 atagttgaac ccagtggcag attgtacact gctagcactg ctagcacaca gtagcaaagc
     2821 aaacagctac attatcttta acataaggca tgtagccata ttttcatata gtggtataca
     2881 acacagtatc attgcaaatg cagtttacag ggatttttga tatactggct tgggacctat
     2941 aagattcttc tcagcagttg ctgaaaagca tgtaaataac tacataatag cctgagaata
     3001 atgggatttt gatagtgcca tttattgtta aagatttatt tttacaaagt aaattcaggg
     3061 ttttagatgt gtacacagct tttacaaatc aataaagatg attgtacaag agtattttca
     3121 gactaattgg attcaaggtt atattctttt aagtgttgtt agtctgcatg cacattggca
     3181 gtaaactagt tacttgtgta ctctgtaata tttgtataat gatcatttct tcttaaggct
     3241 caagatatta gaaaagaaat cagacctaaa taatcattga tgaagtatgt cagttacaag
     3301 cacaaaagaa tataactgct tttataaatc ttttgaatca tgtacaagac ttatatctac
     3361 attttttgat caccagttgt accaaaacac taatttttga aaaagatagg ttaaagtatt
     3421 tgacaaaagt gaatacaata ataacacttg tgtcaaaatg atatagctta gtataaaaat
     3481 aaaattcaac gttttggcca aaatgtttgt ttttcactgg attctgaata taataaattc
     3541 aaagtaccct ttttttagag tttctaataa agtagtatgt aaaaagaaac tgcattttaa
     3601 gtcagacagt aaatgttttt tttaatttac agcaaatgaa gcagttttat tagcatgtta
     3661 caaatattgc caaaccaaga cttctctacc atactttaac agctttcctt cagaaaactc
     3721 tgaagttata tttgcaataa taatgaaacc agcatttagt accagcatat ggttcatatg
     3781 caaataatac tttccccaaa atctttctgg gctaggctat tttcttgcta tgcttcctgc
     3841 acccaaccag cagaattctt ccagggtgga ggaactaatc agcttgttct ccaacagttc
     3901 tgaagccaac ccacaaatct ttgaggaaaa ggggtcactt tgtttactat tctcttggag
     3961 atggaagtga ttctgctctt ccatttgggt atattatttc aagggtattt cttcttaaaa
     4021 atcttggaaa gcctaagaga gttaaaggca tcctggtttt gtttcatttt gctctgtttt
     4081 aaatccatat gcaaataaaa ctgcttctct cttacactgc ttgaactaga aaatcagctt
     4141 tttaatgtag gttaccactt ataagcacaa aagaaacatg tataagaaac cagatttaag
     4201 tgttttaaat aaaatgtaaa cagtttttat ttggtagaga tgactcatgg aaaaattgtg
     4261 ttggcttggt ctgcatgttt aatgatgtgc ttgtatatcg attagctgtg tcacttttaa
     4321 ataagaagtc cacacaagca agccaaattt ttagatgacg aagtccataa ataactagag
     4381 aatttttgtt atctgttgtt aagttgaaat gtataatcat ttatcactaa attgcacatt
     4441 gcctttattt atttgtgctc tgtttttggt ttacagtgta ataatacctc atttaaaaaa
     4501 taaaaaccac tactgttaca ttttattaat ttaaaaagct agaaaattca tgtagttact
     4561 ttttttacat atataatctg ttaatgaatt attgattttt gtatctgcca cagtaaatta
     4621 aagcattaca cagtatttat cagtattttt taaacatcct gtcctttttt aaaatctttg
     4681 cttagtcagt catatttttg tctgtatgat tagaagtttt tacgtccttc cttttttgta
     4741 caaatctgta ttgtattaat ttctggatgc aatttttcaa atattaaaat tatacagtca
     4801 gtcaggcttc agtttatttt ttgaacactt gggcaattaa ttaaagccat atgtttaaaa
     4861 ta
//
