LOCUS       NM_015382               9445 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens HECT domain E3 ubiquitin protein ligase 1 (HECTD1),
            transcript variant 1, mRNA.
ACCESSION   NM_015382
VERSION     NM_015382.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9445)
  AUTHORS   Oxman,E., Li,H., Wang,H.Y. and Zohn,I.E.
  TITLE     Identification and functional analysis of rare HECTD1 missense
            variants in human neural tube defects
  JOURNAL   Hum Genet 143 (3), 263-277 (2024)
   PUBMED   38451291
  REMARK    GeneRIF: Identification and functional analysis of rare HECTD1
            missense variants in human neural tube defects.
REFERENCE   2  (bases 1 to 9445)
  AUTHORS   Zhang,J., Xin,Y., Ling,X., Liang,H., Zhang,L., Fang,C. and Ma,J.
  TITLE     Nucleoporin 93, a new substrate of the E3 ubiquitin protein ligase
            HECTD1, promotes esophageal squamous cell carcinoma progression
  JOURNAL   Hum Cell 37 (1), 245-257 (2024)
   PUBMED   37993750
  REMARK    GeneRIF: Nucleoporin 93, a new substrate of the E3 ubiquitin
            protein ligase HECTD1, promotes esophageal squamous cell carcinoma
            progression.
REFERENCE   3  (bases 1 to 9445)
  AUTHORS   Vaughan,N., Scholz,N., Lindon,C. and Licchesi,J.D.F.
  TITLE     The E3 ubiquitin ligase HECTD1 contributes to cell proliferation
            through an effect on mitosis
  JOURNAL   Sci Rep 12 (1), 13160 (2022)
   PUBMED   35915203
  REMARK    GeneRIF: The E3 ubiquitin ligase HECTD1 contributes to cell
            proliferation through an effect on mitosis.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 9445)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 9445)
  AUTHORS   Harris,L.D., Le Pen,J., Scholz,N., Mieszczanek,J., Vaughan,N.,
            Davis,S., Berridge,G., Kessler,B.M., Bienz,M. and Licchesi,J.D.F.
  TITLE     The deubiquitinase TRABID stabilizes the K29/K48-specific E3
            ubiquitin ligase HECTD1
  JOURNAL   J Biol Chem 296, 100246 (2021)
   PUBMED   33853758
  REMARK    GeneRIF: The deubiquitinase TRABID stabilizes the K29/K48-specific
            E3 ubiquitin ligase HECTD1.
REFERENCE   6  (bases 1 to 9445)
  AUTHORS   Aleidi,S.M., Yang,A., Sharpe,L.J., Rao,G., Cochran,B.J., Rye,K.A.,
            Kockx,M., Brown,A.J. and Gelissen,I.C.
  TITLE     The E3 ubiquitin ligase, HECTD1, is involved in ABCA1-mediated
            cholesterol export from macrophages
  JOURNAL   Biochim Biophys Acta Mol Cell Biol Lipids 1863 (4), 359-368 (2018)
   PUBMED   29306077
  REMARK    GeneRIF: HECTD1 may be involved in the regulation of ABCA1-mediated
            cholesterol export from unloaded macrophages to apoA-I.
REFERENCE   7  (bases 1 to 9445)
  AUTHORS   Fang,S., Guo,H., Cheng,Y., Zhou,Z., Zhang,W., Han,B., Luo,W.,
            Wang,J., Xie,W. and Chao,J.
  TITLE     circHECTD1 promotes the silica-induced pulmonary
            endothelial-mesenchymal transition via HECTD1
  JOURNAL   Cell Death Dis 9 (3), 396 (2018)
   PUBMED   29540674
  REMARK    GeneRIF: SiO2 concomitantly increased circHECTD1 expression, which,
            in turn, inhibited HECTD1 protein expression.
            Publication Status: Online-Only
REFERENCE   8  (bases 1 to 9445)
  AUTHORS   Shen,X., Jia,Z., D'Alonzo,D., Wang,X., Bruder,E., Emch,F.H., De
            Geyter,C. and Zhang,H.
  TITLE     HECTD1 controls the protein level of IQGAP1 to regulate the
            dynamics of adhesive structures
  JOURNAL   Cell Commun Signal 15 (1), 2 (2017)
   PUBMED   28073378
  REMARK    GeneRIF: Hectd1 regulates the protein level of IQGAP1 through
            ubiquitination.
            Publication Status: Online-Only
REFERENCE   9  (bases 1 to 9445)
  AUTHORS   Helander,S., Montecchio,M., Lemak,A., Fares,C., Almlof,J., Yi,Y.,
            Yee,A., Arrowsmith,C., DhePaganon,S. and Sunnerhagen,M.
  TITLE     Basic Tilted Helix Bundle - a new protein fold in human
            FKBP25/FKBP3 and HectD1
  JOURNAL   Biochem Biophys Res Commun 447 (1), 26-31 (2014)
   PUBMED   24667607
  REMARK    GeneRIF: our high-resolution structures show a new fold composed of
            five small helices where H3 and H4 are tilted in a novel
            arrangement; we call this fold the Basic Tilted Helix Bundle (BTHB)
            domain.
REFERENCE   10 (bases 1 to 9445)
  AUTHORS   Tran,H., Bustos,D., Yeh,R., Rubinfeld,B., Lam,C., Shriver,S.,
            Zilberleyb,I., Lee,M.W., Phu,L., Sarkar,A.A., Zohn,I.E.,
            Wertz,I.E., Kirkpatrick,D.S. and Polakis,P.
  TITLE     HectD1 E3 ligase modifies adenomatous polyposis coli (APC) with
            polyubiquitin to promote the APC-axin interaction
  JOURNAL   J Biol Chem 288 (6), 3753-3767 (2013)
   PUBMED   23277359
  REMARK    GeneRIF: HectD1 promotes the APC-Axin interaction to negatively
            regulate Wnt signaling.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL136418.4, DA509926.1, DA818448.1, DA440215.1, AY254380.1,
            AB032957.2 and BM977042.1.
            
            On Nov 22, 2018 this sequence version replaced NM_015382.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660803.155868.1,
                                           SRR1803612.230324.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000399332.6/ ENSP00000382269.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-270               AL136418.4         107854-108123
            271-310             DA509926.1         1-40
            311-872             DA818448.1         1-562
            873-1159            DA440215.1         201-487
            1160-2289           AY254380.1         684-1813
            2290-4640           AB032957.2         166-2516
            4641-6878           AY254380.1         4171-6408
            6879-9444           AB032957.2         4755-7320
            9445-9445           BM977042.1         18-18               c
FEATURES             Location/Qualifiers
     source          1..9445
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q12"
     gene            1..9445
                     /gene="HECTD1"
                     /gene_synonym="EULIR"
                     /note="HECT domain E3 ubiquitin protein ligase 1"
                     /db_xref="GeneID:25831"
                     /db_xref="HGNC:HGNC:20157"
                     /db_xref="MIM:618649"
     exon            1..778
                     /gene="HECTD1"
                     /gene_synonym="EULIR"
                     /inference="alignment:Splign:2.1.0"
     exon            779..939
                     /gene="HECTD1"
                     /gene_synonym="EULIR"
                     /inference="alignment:Splign:2.1.0"
     CDS             800..8632
                     /gene="HECTD1"
                     /gene_synonym="EULIR"
                     /EC_number="2.3.2.26"
                     /note="isoform 1 is encoded by transcript variant 1; E3
                     ubiquitin-protein ligase HECTD1; E3 ligase for inhibin
                     receptor; HECT domain-containing protein 1; HECT domain
                     containing E3 ubiquitin protein ligase 1; HECT-type E3
                     ubiquitin transferase HECTD1"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase HECTD1 isoform 1"
                     /protein_id="NP_056197.3"
                     /db_xref="CCDS:CCDS41939.1"
                     /db_xref="GeneID:25831"
                     /db_xref="HGNC:HGNC:20157"
                     /db_xref="MIM:618649"
                     /translation="MADVDPDTLLEWLQMGQGDERDMQLIALEQLCMLLLMSDNVDRC
                     FETCPPRTFLPALCKIFLDESAPDNVLEVTARAITYYLDVSAECTRRIVGVDGAIKAL
                     CNRLVVVELNNRTSRDLAEQCVKVLELICTRESGAVFEAGGLNCVLTFIRDSGHLVHK
                     DTLHSAMAVVSRLCGKMEPQDSSLEICVESLSSLLKHEDHQVSDGALRCFASLADRFT
                     RRGVDPAPLAKHGLTEELLSRMAAAGGTVSGPSSACKPGRSTTGAPSTTADSKLSNQV
                     STIVSLLSTLCRGSPVVTHDLLRSELPDSIESALQGDERCVLDTMRLVDLLLVLLFEG
                     RKALPKSSAGSTGRIPGLRRLDSSGERSHRQLIDCIRSKDTDALIDAIDTGAFEVNFM
                     DDVGQTLLNWASAFGTQEMVEFLCERGADVNRGQRSSSLHYAACFGRPQVAKTLLRHG
                     ANPDLRDEDGKTPLDKARERGHSEVVAILQSPGDWMCPVNKGDDKKKKDTNKDEEECN
                     EPKGDPEMAPIYLKRLLPVFAQTFQQTMLPSIRKASLALIRKMIHFCSEALLKEVCDS
                     DVGHNLPTILVEITATVLDQEDDDDGHLLALQIIRDLVDKGGDIFLDQLARLGVISKV
                     STLAGPSSDDENEEESKPEKEDEPQEDAKELQQGKPYHWRDWSIIRGRDCLYIWSDAA
                     ALELSNGSNGWFRFILDGKLATMYSSGSPEGGSDSSESRSEFLEKLQRARGQVKPSTS
                     SQPILSAPGPTKLTVGNWSLTCLKEGEIAIHNSDGQQATILKEDLPGFVFESNRGTKH
                     SFTAETSLGSEFVTGWTGKRGRKLKSKLEKTKQKVRTMARDLYDDHFKAVESMPRGVV
                     VTLRNIATQLESSWELHTNRQCIESENTWRDLMKTALENLIVLLKDENTISPYEMCSS
                     GLVQALLTVLNNSMDLDMKQDCSQLVERINVFKTAFSENEDDESRPAVALIRKLIAVL
                     ESIERLPLHLYDTPGSTYNLQILTRRLRFRLERAPGETALIDRTGRMLKMEPLATVES
                     LEQYLLKMVAKQWYDFDRSSFVFVRKLREGQNFIFRHQHDFDENGIIYWIGTNAKTAY
                     EWVNPAAYGLVVVTSSEGRNLPYGRLEDILSRDNSALNCHSNDDKNAWFAIDLGLWVI
                     PSAYTLRHARGYGRSALRNWVFQVSKDGQNWTSLYTHVDDCSLNEPGSTATWPLDPPK
                     DEKQGWRHVRIKQMGKNASGQTHYLSLSGFELYGTVNGVCEDQLGKAAKEAEANLRRQ
                     RRLVRSQVLKYMVPGARVIRGLDWKWRDQDGSPQGEGTVTGELHNGWIDVTWDAGGSN
                     SYRMGAEGKFDLKLAPGYDPDTVASPKPVSSTVSGTTQSWSSLVKNNCPDKTSAAAGS
                     SSRKGSSSSVCSVASSSDISLGSTKTERRSEIVMEHSIVSGADVHEPIVVLSSAENVP
                     QTEVGSSSSASTSTLTAETGSENAERKLGPDSSVRTPGESSAISMGIVSVSSPDVSSV
                     SELTNKEAASQRPLSSSASNRLSVSSLLAAGAPMSSSASVPNLSSRETSSLESFVRRV
                     ANIARTNATNNMNLSRSSSDNNTNTLGRNVMSTATSPLMGAQSFPNLTTPGTTSTVTM
                     STSSVTSSSNVATATTVLSVGQSLSNTLTTSLTSTSSESDTGQEAEYSLYDFLDSCRA
                     STLLAELDDDEDLPEPDEEDDENEDDNQEDQEYEEVMILRRPSLQRRAGSRSDVTHHA
                     VTSQLPQVPAGAGSRPIGEQEEEEYETKGGRRRTWDDDYVLKRQFSALVPAFDPRPGR
                     TNVQQTTDLEIPPPGTPHSELLEEVECTPSPRLALTLKVTGLGTTREVELPLTNFRST
                     IFYYVQKLLQLSCNGNVKSDKLRRIWEPTYTIMYREMKDSDKEKENGKMGCWSIEHVE
                     QYLGTDELPKNDLITYLQKNADAAFLRHWKLTGTNKSIRKNRNCSQLIAAYKDFCEHG
                     TKSGLNQGAISTLQSSDILNLTKEQPQAKAGNGQNSCGVEDVLQLLRILYIVASDPYS
                     RISQEDGDEQPQFTFPPDEFTSKKITTKILQQIEEPLALASGALPDWCEQLTSKCPFL
                     IPFETRQLYFTCTAFGASRAIVWLQNRREATVERTRTTSSVRRDDPGEFRVGRLKHER
                     VKVPRGESLMEWAENVMQIHADRKSVLEVEFLGEEGTGLGPTLEFYALVAAEFQRTDL
                     GAWLCDDNFPDDESRHVDLGGGLKPPGYYVQRSCGLFTAPFPQDSDELERITKLFHFL
                     GIFLAKCIQDNRLVDLPISKPFFKLMCMGDIKSNMSKLIYESRGDRDLHCTESQSEAS
                     TEEGHDSLSVGSFEEDSKSEFILDPPKPKPPAWFNGILTWEDFELVNPHRARFLKEIK
                     DLAIKRRQILSNKGLSEDEKNTKLQELVLKNPSGSGPPLSIEDLGLNFQFCPSSRIYG
                     FTAVDLKPSGEDEMITMDNAEEYVDLMFDFCMHTGIQKQMEAFRDGFNKVFPMEKLSS
                     FSHEEVQMILCGNQSPSWAAEDIINYTEPKLGYTRDSPGFLRFVRVLCGMSSDERKAF
                     LQFTTGCSTLPPGGLANLHPRLTVVRKVDATDASYPSVNTCVHYLKLPEYSSEEIMRE
                     RLLAATMEKGFHLN"
     exon            940..1174
                     /gene="HECTD1"
                     /gene_synonym="EULIR"
                     /inference="alignment:Splign:2.1.0"
     exon            1175..1402
                     /gene="HECTD1"
                     /gene_synonym="EULIR"
                     /inference="alignment:Splign:2.1.0"
     exon            1403..1684
                     /gene="HECTD1"
                     /gene_synonym="EULIR"
                     /inference="alignment:Splign:2.1.0"
     exon            1685..1955
                     /gene="HECTD1"
                     /gene_synonym="EULIR"
                     /inference="alignment:Splign:2.1.0"
     exon            1956..2032
                     /gene="HECTD1"
                     /gene_synonym="EULIR"
                     /inference="alignment:Splign:2.1.0"
     exon            2033..2131
                     /gene="HECTD1"
                     /gene_synonym="EULIR"
                     /inference="alignment:Splign:2.1.0"
     exon            2132..2246
                     /gene="HECTD1"
                     /gene_synonym="EULIR"
                     /inference="alignment:Splign:2.1.0"
     exon            2247..2421
                     /gene="HECTD1"
                     /gene_synonym="EULIR"
                     /inference="alignment:Splign:2.1.0"
     exon            2422..2560
                     /gene="HECTD1"
                     /gene_synonym="EULIR"
                     /inference="alignment:Splign:2.1.0"
     exon            2561..2731
                     /gene="HECTD1"
                     /gene_synonym="EULIR"
                     /inference="alignment:Splign:2.1.0"
     exon            2732..2954
                     /gene="HECTD1"
                     /gene_synonym="EULIR"
                     /inference="alignment:Splign:2.1.0"
     exon            2955..3218
                     /gene="HECTD1"
                     /gene_synonym="EULIR"
                     /inference="alignment:Splign:2.1.0"
     exon            3219..3295
                     /gene="HECTD1"
                     /gene_synonym="EULIR"
                     /inference="alignment:Splign:2.1.0"
     exon            3296..3430
                     /gene="HECTD1"
                     /gene_synonym="EULIR"
                     /inference="alignment:Splign:2.1.0"
     exon            3431..3577
                     /gene="HECTD1"
                     /gene_synonym="EULIR"
                     /inference="alignment:Splign:2.1.0"
     exon            3578..3672
                     /gene="HECTD1"
                     /gene_synonym="EULIR"
                     /inference="alignment:Splign:2.1.0"
     exon            3673..3778
                     /gene="HECTD1"
                     /gene_synonym="EULIR"
                     /inference="alignment:Splign:2.1.0"
     exon            3779..3913
                     /gene="HECTD1"
                     /gene_synonym="EULIR"
                     /inference="alignment:Splign:2.1.0"
     exon            3914..4053
                     /gene="HECTD1"
                     /gene_synonym="EULIR"
                     /inference="alignment:Splign:2.1.0"
     exon            4054..4377
                     /gene="HECTD1"
                     /gene_synonym="EULIR"
                     /inference="alignment:Splign:2.1.0"
     exon            4378..4544
                     /gene="HECTD1"
                     /gene_synonym="EULIR"
                     /inference="alignment:Splign:2.1.0"
     exon            4545..4721
                     /gene="HECTD1"
                     /gene_synonym="EULIR"
                     /inference="alignment:Splign:2.1.0"
     exon            4722..5558
                     /gene="HECTD1"
                     /gene_synonym="EULIR"
                     /inference="alignment:Splign:2.1.0"
     exon            5559..5780
                     /gene="HECTD1"
                     /gene_synonym="EULIR"
                     /inference="alignment:Splign:2.1.0"
     exon            5781..5914
                     /gene="HECTD1"
                     /gene_synonym="EULIR"
                     /inference="alignment:Splign:2.1.0"
     exon            5915..6037
                     /gene="HECTD1"
                     /gene_synonym="EULIR"
                     /inference="alignment:Splign:2.1.0"
     exon            6038..6194
                     /gene="HECTD1"
                     /gene_synonym="EULIR"
                     /inference="alignment:Splign:2.1.0"
     exon            6195..6417
                     /gene="HECTD1"
                     /gene_synonym="EULIR"
                     /inference="alignment:Splign:2.1.0"
     exon            6418..6472
                     /gene="HECTD1"
                     /gene_synonym="EULIR"
                     /inference="alignment:Splign:2.1.0"
     exon            6473..6655
                     /gene="HECTD1"
                     /gene_synonym="EULIR"
                     /inference="alignment:Splign:2.1.0"
     exon            6656..6863
                     /gene="HECTD1"
                     /gene_synonym="EULIR"
                     /inference="alignment:Splign:2.1.0"
     exon            6864..6949
                     /gene="HECTD1"
                     /gene_synonym="EULIR"
                     /inference="alignment:Splign:2.1.0"
     exon            6950..7077
                     /gene="HECTD1"
                     /gene_synonym="EULIR"
                     /inference="alignment:Splign:2.1.0"
     exon            7078..7279
                     /gene="HECTD1"
                     /gene_synonym="EULIR"
                     /inference="alignment:Splign:2.1.0"
     exon            7280..7417
                     /gene="HECTD1"
                     /gene_synonym="EULIR"
                     /inference="alignment:Splign:2.1.0"
     exon            7418..8025
                     /gene="HECTD1"
                     /gene_synonym="EULIR"
                     /inference="alignment:Splign:2.1.0"
     exon            8026..8104
                     /gene="HECTD1"
                     /gene_synonym="EULIR"
                     /inference="alignment:Splign:2.1.0"
     exon            8105..8198
                     /gene="HECTD1"
                     /gene_synonym="EULIR"
                     /inference="alignment:Splign:2.1.0"
     exon            8199..8349
                     /gene="HECTD1"
                     /gene_synonym="EULIR"
                     /inference="alignment:Splign:2.1.0"
     exon            8350..8494
                     /gene="HECTD1"
                     /gene_synonym="EULIR"
                     /inference="alignment:Splign:2.1.0"
     exon            8495..9445
                     /gene="HECTD1"
                     /gene_synonym="EULIR"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9426..9431
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HECTD1"
                     /gene_synonym="EULIR"
                     /note="hexamer: ATTAAA"
     polyA_site      9445
                     /gene="HECTD1"
                     /gene_synonym="EULIR"
                     /note="major polyA site"
ORIGIN      
        1 gcctccgccg ggctcggctg ccctcggcag cttccccgcc tggctctccc tcgaccctcc
       61 tcccctcccc ctcccggttc gcgatggact gggcctcccc gccgcgccgc cgccgccgtc
      121 gcccgcagca ccgcgcgggc ctctgagttg cagggcctga ggagccgcgt gctggggact
      181 ggacgtccgc tggctgctgc cgcacgcgag aacctctcgc ttccctgtca ccaggtcctc
      241 gcggaggaag gggagggacg aggcgcggct tttcctgtgc cgcctgcccg gttcgacctg
      301 cgcggcctgc ggcttcttgg gaactctagg gccgcggccg ggcctggctc tgccggcggc
      361 ctgttgggag ctggatcgga gcggtttgga acgacaagcc cgagtaagag cctgcgggaa
      421 ggggaggcgg gagcgcctcg gaccgtcgga agggccctgc gtgggggcgc ggatcccgga
      481 cgcccggcga gtggcggaaa gcgagacccc ggcgccgagt gaggtcggcc aggctgctgc
      541 cgacttcccc gctggcccct ttgttcccct cccggggccc tgccggcggc ggggcccggt
      601 tgcccgcgcc agagcctcag gccggctcct tttgcccttg agaagggttc gctgccaggc
      661 cttcgagttc cgggcgcagc tcccgggaga gccggcgccc tgcctggccc ggcggcctcc
      721 tctcgggcac gaaggcttgc tgctgcttct ccagaacttc ccttcctcgt agacacagca
      781 aagagacttt taaaaaacca tggcagatgt ggacccagat acattgctgg aatggctaca
      841 gatgggacag ggagatgaaa gggacatgca actaatagcc cttgaacagc tatgcatgct
      901 gcttttgatg tctgacaacg tggatcgttg ttttgaaaca tgtcctcctc gcactttctt
      961 accagccctt tgcaaaattt ttcttgatga aagtgctcca gacaatgtat tagaggtgac
     1021 agcccgtgcc ataacatact acctggatgt atctgcggaa tgtacccgaa ggattgttgg
     1081 ggtagatgga gctataaaag cactttgtaa tcgtttggtt gtagttgaac ttaacaacag
     1141 gactagcaga gacttagccg aacagtgtgt aaaggtatta gaactgatat gtactcgtga
     1201 gtcaggagca gtctttgagg ctggtggttt gaattgtgtg cttaccttca ttcgtgacag
     1261 tggacatcta gttcataaag acaccttgca ctctgctatg gctgtggtat caagactctg
     1321 tggcaaaatg gagcctcaag attcttcttt agaaatttgt gtagaatctc tgtctagttt
     1381 attaaagcat gaagatcatc aggtttcaga tggagctctg cgatgctttg catcactggc
     1441 tgaccgattt acccgtcgtg gtgttgaccc agctccatta gccaagcatg gattaactga
     1501 ggagctgtta tctcgaatgg ctgctgctgg tggtactgtt tcaggaccat catcagcatg
     1561 caaaccaggt cgcagcacca caggagctcc atccaccact gcagattcca aattgagtaa
     1621 tcaggtgtca acaattgtaa gtctgctctc aacactttgc agaggctctc cggtagtaac
     1681 acatgatctt ctgaggtcgg agcttccaga ttcaattgaa agtgcattgc agggtgatga
     1741 aagatgtgtg cttgatacta tgcgtttggt tgaccttctc ttggtgctat tatttgaagg
     1801 acgaaaagct ttgccaaagt ctagtgctgg atctacaggc agaatcccag gactccggag
     1861 attagatagt tctggggagc gctcacatcg gcagcttata gattgtattc gaagtaaaga
     1921 taccgatgca cttatagatg caattgacac aggagccttt gaagtaaatt ttatggatga
     1981 tgtaggtcag actctattaa actgggcctc tgcttttgga actcaggaaa tggtagaatt
     2041 tctttgtgag agaggtgcag atgttaatag aggtcaaagg tcatcatcat tacattatgc
     2101 tgcatgtttt ggaagacctc aagtagcaaa gactctgtta cggcatggtg caaatccaga
     2161 tctgagagat gaagatggga aaactccatt agataaagct cgagaaaggg gccatagtga
     2221 agtggtagct attcttcagt ctccaggtga ttggatgtgt ccagttaata aaggagatga
     2281 taagaaaaag aaagatacaa acaaagatga agaagaatgt aatgagccca aaggagatcc
     2341 ggaaatggca cccatatact tgaaaaggtt attgccagtg tttgcacaaa catttcagca
     2401 aactatgctg ccttcaataa ggaaagcaag tcttgctcta attcgaaaaa tgattcattt
     2461 ttgctctgaa gcactgttaa aagaagtttg tgattctgat gttggtcaca atttgcctac
     2521 aatactagtg gaaatcactg caactgtcct ggatcaagag gatgatgatg atggccactt
     2581 gctggctttg cagatcataa gggatttagt agataaaggt ggtgatatat ttttggatca
     2641 gctagccaga cttggtgtaa ttagcaaagt gtcaacgttg gcaggtcctt cctctgatga
     2701 tgagaatgaa gaggaatcaa aaccagaaaa agaagatgaa ccacaggaag atgctaaaga
     2761 attgcaacaa ggtaaaccat atcattggag agactggtca atcattaggg gaagggactg
     2821 cttatatatt tggagtgatg cagcagcctt ggaattatct aatggcagta atggatggtt
     2881 cagatttatc ttggatggaa aacttgccac catgtattca agtggtagtc cggaaggtgg
     2941 atctgacagt tcagaaagcc gaagtgaatt cttagagaag ttacaaagag ctcgaggcca
     3001 agtaaagcca tctacttcaa gtcaacctat actgtcagca ccaggaccca ctaaacttac
     3061 tgtaggaaat tggtcactga catgtttgaa agaaggagaa attgctattc ataattcaga
     3121 tggtcagcaa gctacaatat tgaaagaaga tttacctggt tttgtatttg aatctaatag
     3181 aggaaccaaa cattcattta ctgcagaaac ttccctgggt tcagaatttg tgactggctg
     3241 gactggcaaa agaggcagaa aactgaaatc taagttagaa aaaacaaagc aaaaggtacg
     3301 aactatggct cgagatttat acgatgacca ttttaaagct gttgaaagca tgcctcgtgg
     3361 agtagtggta acactcagaa acatagcaac tcagttagag tcatcttggg aacttcatac
     3421 aaatagacaa tgtattgaga gtgagaacac ttggagagat ttaatgaaga cagctttaga
     3481 aaacctaatt gtacttttga aggatgaaaa cacaatttca ccatatgaaa tgtgtagcag
     3541 tggcttggta caagcacttc ttactgtgtt aaacaatagc atggatttgg atatgaaaca
     3601 agattgtagt caactggtag aaagaataaa tgtttttaaa actgccttta gtgaaaatga
     3661 agatgatgaa agtcgaccag cagttgcgtt aattcgaaag ttaatagctg tactagaatc
     3721 tattgaacgt ctacctctcc atttgtatga tacaccagga tccacatata acctccagat
     3781 acttacaagg agattacgat ttcggttgga acgtgcacct ggtgaaactg cattgattga
     3841 caggactggc agaatgttga agatggaacc tttggctaca gttgaatctc tggaacagta
     3901 ccttttgaaa atggtagcaa aacagtggta tgattttgac cgatcttcat ttgtttttgt
     3961 tcgaaaatta agagaaggac aaaattttat atttcggcac cagcatgatt ttgatgaaaa
     4021 tggaatcatt tactggattg gaacaaatgc aaaaactgct tatgaatggg taaatccagc
     4081 tgcctatgga cttgtagtag taacgtcatc agaaggaaga aatctacctt atggccgctt
     4141 agaagacata ctaagtcgtg ataattcagc tttaaattgt catagcaatg atgataagaa
     4201 tgcctggttt gccatagatc tgggtctctg ggtgatacca tcagcatata cacttcgtca
     4261 tgctcgtggt tatggaaggt ctgcactgag aaattgggtt ttccaggtat ccaaagatgg
     4321 acagaactgg acttctttgt atacccatgt tgatgactgc agtctcaatg aaccagggtc
     4381 aactgcaact tggcctcttg atccaccaaa ggatgagaaa caagggtgga gacatgtgag
     4441 aattaaacag atggggaaaa atgccagtgg acaaacacac tacctctcat tatctggatt
     4501 cgaactttat ggcactgtaa atggagtatg tgaagatcag ctagggaaag cagctaaaga
     4561 agcagaagct aatcttagac ggcagagacg tctagtacgt tcccaggttc tgaaatacat
     4621 ggttccagga gctcgtgtta tcagaggcct ggattggaaa tggcgagatc aggatggcag
     4681 cccacaggga gaaggcactg tcacaggaga actacacaat ggctggattg atgtcacctg
     4741 ggatgctggt ggctcaaact cttaccgtat gggcgcagaa ggaaaatttg acctcaagct
     4801 tgcaccaggg tacgaccctg atacagtggc atcacccaaa cctgtttcat ccactgtttc
     4861 aggcacaacg caatcatgga gcagcttggt gaaaaacaac tgtccagaca agacatctgc
     4921 tgctgcaggc tcctcaagta gaaaaggaag cagcagttct gtgtgtagcg tggccagtag
     4981 cagcgacatc agcttgggtt cgaccaaaac ggaacggaga tcagaaattg taatggaaca
     5041 cagtatagtt tcaggagctg atgtccatga accaattgtt gttctttcat ctgctgaaaa
     5101 cgtccctcaa acagaagtag ggtcatcttc cagtgcaagc accagcacct taacagcgga
     5161 aacgggaagt gaaaatgctg aaaggaagtt aggccctgat agttctgttc gtactcctgg
     5221 ggagtctagt gcaatatcca tgggaattgt cagtgttagt tctcctgatg ttagttcagt
     5281 atctgaatta actaataaag aagcagcttc acaacgacct cttagctctt cagcaagtaa
     5341 cagactgtca gtgagttctt tgttggctgc tggggcccct atgagctcta gtgcaagtgt
     5401 acctaacctg tcctcaagag aaacatctag cttggagagt tttgtaagga gagtggcaaa
     5461 catagcacgg actaatgcca cgaacaacat gaatctaagc cgaagcagca gtgataacaa
     5521 cactaatact ttggggagga atgtgatgag cacagcaact tctcctctta tgggtgctca
     5581 gagtttccct aatttgacca cacctggtac tacatcaaca gtgactatgt caacatccag
     5641 tgttactagc agcagcaatg tagctacagc aacaacagtt ttatcagttg gtcaatcttt
     5701 aagtaacact ttaaccacca gcctcacatc aacttccagt gagagtgaca caggtcagga
     5761 agcagaatat tccttatatg atttccttga tagctgccgt gccagtactc tattggctga
     5821 gctcgatgat gatgaggact tacctgagcc agatgaagaa gatgatgaga atgaagatga
     5881 caatcaggag gaccaagaat acgaggaggt tatgattctg agacgcccat ccctgcaacg
     5941 tcgagctggc tcccgctctg atgtaacgca tcatgctgtt acctcgcagc taccacaggt
     6001 acctgctgga gcagggagcc gacctattgg ggagcaggaa gaagaagagt acgaaactaa
     6061 aggaggacgc cggagaacat gggatgatga ttatgtgcta aagagacagt tttctgcatt
     6121 ggttcctgct tttgatccta gacctggtcg tactaatgtc cagcagacaa ctgatctaga
     6181 aataccaccc ccagggaccc ctcattcaga gctcttggaa gaagtcgaat gtactccgtc
     6241 acctcgatta gctctcactt tgaaagtaac aggtcttgga acgactcgtg aagttgaatt
     6301 accactcacc aatttcagat caaccatctt ttactatgta caaaaattgc ttcaattgtc
     6361 ctgtaatggc aatgtgaaat cagataaact taggcgtatt tgggagccca catacacaat
     6421 catgtacaga gaaatgaagg attctgataa agaaaaggaa aatggaaaaa tgggttgctg
     6481 gtctatagag catgtggagc agtaccttgg cactgatgaa ttaccaaaga atgacttgat
     6541 aacctacctg cagaagaatg cagacgctgc tttcctgcgc cactggaaat taactggcac
     6601 taataaaagt attaggaaaa acagaaattg ttctcagctc atagctgcat ataaggattt
     6661 ttgtgagcat ggaacaaagt ctgggttaaa ccagggggcc atttctactc ttcaaagtag
     6721 tgatattctt aatttaacaa aagaacaacc tcaggccaaa gcaggcaatg gacagaactc
     6781 ttgtggagta gaagatgtcc ttcagcttct gcgtattcta tatatagttg caagtgaccc
     6841 ttattcaaga atatcccagg aagatggtga tgaacagcct cagtttactt ttccaccaga
     6901 tgaattcact agcaaaaaaa ttacaacaaa aatattacag cagattgagg aaccattggc
     6961 actggcaagt ggggctctgc cagactggtg tgaacaatta accagcaaat gtccttttct
     7021 aataccattt gaaactagac agctttattt cacatgtaca gcatttggcg cctcaagagc
     7081 aatagtatgg ttacagaacc gacgtgaagc cactgtggag cgaacgagaa ccacaagcag
     7141 tgttaggcga gatgaccctg gagagtttcg agttggtcgt ctcaagcatg aaagagtaaa
     7201 agttccacgt ggcgagtcac tgatggaatg ggctgagaat gtcatgcaaa tacatgcaga
     7261 tcggaaatca gttcttgagg ttgaattttt aggagaagaa ggaactggct tgggacccac
     7321 attagagttt tatgctctgg tggcagcaga attccagaga actgacttgg gagcttggct
     7381 ttgtgatgat aattttccag atgatgaatc tcgtcacgtt gatcttggag gtggattgaa
     7441 acctcctgga tattatgtgc agaggtcatg tggactgttc acagcaccat ttccacagga
     7501 tagtgatgag cttgaaagga tcacgaaact gtttcatttc cttggaattt tcttggccaa
     7561 atgcattcaa gacaatagac ttgtggactt acctatttct aaaccttttt ttaaacttat
     7621 gtgtatgggt gacattaaaa gcaatatgag taaactgatt tatgagtcac gaggtgatag
     7681 agacttacac tgtactgaaa gtcagtctga agcttctaca gaagaaggtc atgattcact
     7741 ctcggtagga agctttgaag aggattcaaa atcagaattt attcttgatc cccctaaacc
     7801 aaaaccccca gcttggttta atggaatttt gacttgggaa gactttgaat tagtaaaccc
     7861 acacagagcc agatttttaa aagaaattaa agaccttgct atcaagaggc gccaaatttt
     7921 aagcaacaaa ggtctttctg aagatgagaa gaacacaaaa ttacaggaac tagtgctgaa
     7981 gaatccatca ggttctgggc ctccacttag catagaggat ttaggtttaa atttccagtt
     8041 ttgcccttcc tcaagaatat atggttttac agctgtggat ctcaagccaa gtggtgaaga
     8101 tgagatgata acaatggata atgcagaaga atatgtggat ttgatgtttg acttttgtat
     8161 gcatacgggt attcagaaac aaatggaagc ctttagagat gggtttaata aagtttttcc
     8221 aatggagaaa ttaagttcct tcagccatga agaagtccaa atgattcttt gtggaaacca
     8281 gtcaccatcc tgggcagcag aggatattat caattacact gaacctaagc tgggttatac
     8341 acgtgacagc cctggtttcc tgaggtttgt gagggtttta tgtggcatgt cttctgatga
     8401 aaggaaagca ttcttgcagt ttaccactgg ttgttcaact ctacccccag gtggactggc
     8461 taacctgcat cccaggctca cggttgtacg caaggttgat gctactgatg caagctatcc
     8521 atcagtcaat acatgtgtgc attaccttaa gttgcctgaa tattcttccg aggagatcat
     8581 gagagagcgc ctgctagctg ctacaatgga gaaaggcttt catctcaatt gagctttgaa
     8641 gtgcaatggg agacatcaga gactttaaaa atactagtga agcctcttgt gtttgtgtgc
     8701 agagaagtat atgatccacc atgctaatga cacttgcctt tttttccacc attaaggctt
     8761 taagaacatg tggaataagt tttttagctg ctaatgacaa aacaaatcct gtaactaccc
     8821 agccagcaag tatatagcac agaacactgt gttactttac aagggcttat gtgactggaa
     8881 taaggtggtc ccacttgact gttccaaaga gcagcttctc agatcttcag tgttcactgg
     8941 taaatttcta acagtgtatt tgtgtaaagt ttgtcatttc atactccata cactacagtt
     9001 gctgtcactg atccctgttt tgctggcttt taagctactt ggtcaaaaat cctgcttcct
     9061 taaaacatag agaattaatg agcatctcaa gctttttctt ttccttttta atgatgcctg
     9121 cactatcaag agtattctag tgttctctct ttgtttggca tataatcatg caccaaactt
     9181 tttatttctt taaggtggga gtatattttt atttcctaaa tgccatacta tgaagatcaa
     9241 agtcttaagt gtgtttgcag ctcaaaaata aagatgtatt aaggggggaa aacctggtct
     9301 aagtgcaagg cacacttaca gcgagtttta ctttcggttg tattttcttt gtatattata
     9361 aacatttatt taacttgttg ccgtttgaag taaaaaattt ccaaaatgta tgctcaacaa
     9421 taatcattaa aatgtttgca gcgta
//
