LOCUS       NM_001527               9737 bp    mRNA    linear   PRI 01-OCT-2024
DEFINITION  Homo sapiens histone deacetylase 2 (HDAC2), transcript variant 1,
            mRNA.
ACCESSION   NM_001527
VERSION     NM_001527.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9737)
  AUTHORS   Zhan,F., Zhang,R., Qiu,L. and Ren,Y.
  TITLE     ACAP3 negatively regulated by HDAC2 inhibits the malignant
            development of papillary thyroid carcinoma cells
  JOURNAL   Int J Biochem Cell Biol 174, 106635 (2024)
   PUBMED   39098591
  REMARK    GeneRIF: ACAP3 negatively regulated by HDAC2 inhibits the malignant
            development of papillary thyroid carcinoma cells.
REFERENCE   2  (bases 1 to 9737)
  AUTHORS   Wang,M., Liao,J., Wang,J., Xu,M., Cheng,Y., Wei,L. and Huang,A.
  TITLE     HDAC2 promotes autophagy-associated HCC malignant progression by
            transcriptionally activating LAPTM4B
  JOURNAL   Cell Death Dis 15 (8), 593 (2024)
   PUBMED   39147759
  REMARK    GeneRIF: HDAC2 promotes autophagy-associated HCC malignant
            progression by transcriptionally activating LAPTM4B.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 9737)
  AUTHORS   Di Giorgio,E., Dalla,E., Tolotto,V., D'Este,F., Paluvai,H.,
            Ranzino,L. and Brancolini,C.
  TITLE     HDAC4 influences the DNA damage response and counteracts senescence
            by assembling with HDAC1/HDAC2 to control H2BK120 acetylation and
            homology-directed repair
  JOURNAL   Nucleic Acids Res 52 (14), 8218-8240 (2024)
   PUBMED   38874468
  REMARK    GeneRIF: HDAC4 influences the DNA damage response and counteracts
            senescence by assembling with HDAC1/HDAC2 to control H2BK120
            acetylation and homology-directed repair.
REFERENCE   4  (bases 1 to 9737)
  AUTHORS   Maciejewski,K., Giers,M., Oleksiewicz,U. and Czerwinska,P.
  TITLE     The Epigenetic Modifiers HDAC2 and HDAC7 Inversely Associate with
            Cancer Stemness and Immunity in Solid Tumors
  JOURNAL   Int J Mol Sci 25 (14), 7841 (2024)
   PUBMED   39063083
  REMARK    GeneRIF: The Epigenetic Modifiers HDAC2 and HDAC7 Inversely
            Associate with Cancer Stemness and Immunity in Solid Tumors.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 9737)
  AUTHORS   Guan,X., Liu,R., Wang,B., Xiong,R., Cui,L., Liao,Y., Ruan,Y.,
            Fang,L., Lu,X., Yu,X., Su,D., Ma,Y., Dang,T., Chen,Z., Yao,Y.,
            Liu,C. and Zhang,Y.
  TITLE     Inhibition of HDAC2 sensitises antitumour therapy by promoting
            NLRP3/GSDMD-mediated pyroptosis in colorectal cancer
  JOURNAL   Clin Transl Med 14 (6), e1692 (2024)
   PUBMED   38804602
  REMARK    GeneRIF: Inhibition of HDAC2 sensitises antitumour therapy by
            promoting NLRP3/GSDMD-mediated pyroptosis in colorectal cancer.
REFERENCE   6  (bases 1 to 9737)
  AUTHORS   Hassig,C.A., Tong,J.K., Fleischer,T.C., Owa,T., Grable,P.G.,
            Ayer,D.E. and Schreiber,S.L.
  TITLE     A role for histone deacetylase activity in HDAC1-mediated
            transcriptional repression
  JOURNAL   Proc Natl Acad Sci U S A 95 (7), 3519-3524 (1998)
   PUBMED   9520398
REFERENCE   7  (bases 1 to 9737)
  AUTHORS   Yang,W.M., Yao,Y.L., Sun,J.M., Davie,J.R. and Seto,E.
  TITLE     Isolation and characterization of cDNAs corresponding to an
            additional member of the human histone deacetylase gene family
  JOURNAL   J Biol Chem 272 (44), 28001-28007 (1997)
   PUBMED   9346952
REFERENCE   8  (bases 1 to 9737)
  AUTHORS   Zhang,Y., Iratni,R., Erdjument-Bromage,H., Tempst,P. and
            Reinberg,D.
  TITLE     Histone deacetylases and SAP18, a novel polypeptide, are components
            of a human Sin3 complex
  JOURNAL   Cell 89 (3), 357-364 (1997)
   PUBMED   9150135
REFERENCE   9  (bases 1 to 9737)
  AUTHORS   Laherty,C.D., Yang,W.M., Sun,J.M., Davie,J.R., Seto,E. and
            Eisenman,R.N.
  TITLE     Histone deacetylases associated with the mSin3 corepressor mediate
            mad transcriptional repression
  JOURNAL   Cell 89 (3), 349-356 (1997)
   PUBMED   9150134
  REMARK    GeneRIF: mSin3A interacts with HDAC2 via the HDAC-interacting
            domain (HID) of mSin3A.
REFERENCE   10 (bases 1 to 9737)
  AUTHORS   Yang,W.M., Inouye,C., Zeng,Y., Bearss,D. and Seto,E.
  TITLE     Transcriptional repression by YY1 is mediated by interaction with a
            mammalian homolog of the yeast global regulator RPD3
  JOURNAL   Proc Natl Acad Sci U S A 93 (23), 12845-12850 (1996)
   PUBMED   8917507
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK296856.1, BC031055.1 and
            FO393415.1.
            
            On Nov 24, 2018 this sequence version replaced NM_001527.3.
            
            Summary: This gene product belongs to the histone deacetylase
            family. Histone deacetylases act via the formation of large
            multiprotein complexes, and are responsible for the deacetylation
            of lysine residues at the N-terminal regions of core histones (H2A,
            H2B, H3 and H4). This protein forms transcriptional repressor
            complexes by associating with many different proteins, including
            YY1, a mammalian zinc-finger transcription factor. Thus, it plays
            an important role in transcriptional regulation, cell cycle
            progression and developmental events. Alternative splicing results
            in multiple transcript variants. [provided by RefSeq, Apr 2010].
            
            Transcript Variant: This variant (1) encodes the functional
            protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038192.896268.1,
                                           SRR14038196.2566080.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000519065.6/ ENSP00000430432.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-982               AK296856.1         48-1029
            983-1182            BC031055.1         994-1193
            1183-1996           AK296856.1         1230-2043
            1997-9737           FO393415.1         160176-167916       c
FEATURES             Location/Qualifiers
     source          1..9737
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q21"
     gene            1..9737
                     /gene="HDAC2"
                     /gene_synonym="HD2; KDAC2; RPD3; YAF1"
                     /note="histone deacetylase 2"
                     /db_xref="GeneID:3066"
                     /db_xref="HGNC:HGNC:4853"
                     /db_xref="MIM:605164"
     exon            1..292
                     /gene="HDAC2"
                     /gene_synonym="HD2; KDAC2; RPD3; YAF1"
                     /inference="alignment:Splign:2.1.0"
     CDS             241..1707
                     /gene="HDAC2"
                     /gene_synonym="HD2; KDAC2; RPD3; YAF1"
                     /EC_number="3.5.1.98"
                     /note="transcriptional regulator homolog RPD3;
                     YY1-associated factor 1; protein deacylase HDAC2"
                     /codon_start=1
                     /product="histone deacetylase 2"
                     /protein_id="NP_001518.3"
                     /db_xref="CCDS:CCDS43493.2"
                     /db_xref="GeneID:3066"
                     /db_xref="HGNC:HGNC:4853"
                     /db_xref="MIM:605164"
                     /translation="MAYSQGGGKKKVCYYYDGDIGNYYYGQGHPMKPHRIRMTHNLLL
                     NYGLYRKMEIYRPHKATAEEMTKYHSDEYIKFLRSIRPDNMSEYSKQMQRFNVGEDCP
                     VFDGLFEFCQLSTGGSVAGAVKLNRQQTDMAVNWAGGLHHAKKSEASGFCYVNDIVLA
                     ILELLKYHQRVLYIDIDIHHGDGVEEAFYTTDRVMTVSFHKYGEYFPGTGDLRDIGAG
                     KGKYYAVNFPMRDGIDDESYGQIFKPIISKVMEMYQPSAVVLQCGADSLSGDRLGCFN
                     LTVKGHAKCVEVVKTFNLPLLMLGGGGYTIRNVARCWTYETAVALDCEIPNELPYNDY
                     FEYFGPDFKLHISPSNMTNQNTPEYMEKIKQRLFENLRMLPHAPGVQMQAIPEDAVHE
                     DSGDEDGEDPDKRISIRASDKRIACDEEFSDSEDEGEGGRRNVADHKKGAKKARIEED
                     KKETEDKKTDVKEEDKSKDNSGEKTDTKGTKSEQLSNP"
     exon            293..405
                     /gene="HDAC2"
                     /gene_synonym="HD2; KDAC2; RPD3; YAF1"
                     /inference="alignment:Splign:2.1.0"
     exon            406..523
                     /gene="HDAC2"
                     /gene_synonym="HD2; KDAC2; RPD3; YAF1"
                     /inference="alignment:Splign:2.1.0"
     exon            524..598
                     /gene="HDAC2"
                     /gene_synonym="HD2; KDAC2; RPD3; YAF1"
                     /inference="alignment:Splign:2.1.0"
     exon            599..737
                     /gene="HDAC2"
                     /gene_synonym="HD2; KDAC2; RPD3; YAF1"
                     /inference="alignment:Splign:2.1.0"
     exon            738..879
                     /gene="HDAC2"
                     /gene_synonym="HD2; KDAC2; RPD3; YAF1"
                     /inference="alignment:Splign:2.1.0"
     exon            880..972
                     /gene="HDAC2"
                     /gene_synonym="HD2; KDAC2; RPD3; YAF1"
                     /inference="alignment:Splign:2.1.0"
     exon            973..1081
                     /gene="HDAC2"
                     /gene_synonym="HD2; KDAC2; RPD3; YAF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1082..1222
                     /gene="HDAC2"
                     /gene_synonym="HD2; KDAC2; RPD3; YAF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1223..1331
                     /gene="HDAC2"
                     /gene_synonym="HD2; KDAC2; RPD3; YAF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1332..1462
                     /gene="HDAC2"
                     /gene_synonym="HD2; KDAC2; RPD3; YAF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1463..1618
                     /gene="HDAC2"
                     /gene_synonym="HD2; KDAC2; RPD3; YAF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1619..1676
                     /gene="HDAC2"
                     /gene_synonym="HD2; KDAC2; RPD3; YAF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1677..9737
                     /gene="HDAC2"
                     /gene_synonym="HD2; KDAC2; RPD3; YAF1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2029..2034
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HDAC2"
                     /gene_synonym="HD2; KDAC2; RPD3; YAF1"
                     /note="hexamer: ATTAAA"
     polyA_site      2048
                     /gene="HDAC2"
                     /gene_synonym="HD2; KDAC2; RPD3; YAF1"
                     /note="major polyA site"
     regulatory      3933..3938
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HDAC2"
                     /gene_synonym="HD2; KDAC2; RPD3; YAF1"
                     /note="hexamer: AATAAA"
     polyA_site      3953
                     /gene="HDAC2"
                     /gene_synonym="HD2; KDAC2; RPD3; YAF1"
     regulatory      6584..6589
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HDAC2"
                     /gene_synonym="HD2; KDAC2; RPD3; YAF1"
                     /note="hexamer: ATTAAA"
     regulatory      6598..6603
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HDAC2"
                     /gene_synonym="HD2; KDAC2; RPD3; YAF1"
                     /note="hexamer: ATTAAA"
     polyA_site      6609
                     /gene="HDAC2"
                     /gene_synonym="HD2; KDAC2; RPD3; YAF1"
     polyA_site      6618
                     /gene="HDAC2"
                     /gene_synonym="HD2; KDAC2; RPD3; YAF1"
     regulatory      8056..8061
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HDAC2"
                     /gene_synonym="HD2; KDAC2; RPD3; YAF1"
                     /note="hexamer: AGTAAA"
     polyA_site      8088
                     /gene="HDAC2"
                     /gene_synonym="HD2; KDAC2; RPD3; YAF1"
     regulatory      8176..8181
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HDAC2"
                     /gene_synonym="HD2; KDAC2; RPD3; YAF1"
                     /note="hexamer: AATAAA"
     polyA_site      8201
                     /gene="HDAC2"
                     /gene_synonym="HD2; KDAC2; RPD3; YAF1"
     regulatory      9719..9724
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HDAC2"
                     /gene_synonym="HD2; KDAC2; RPD3; YAF1"
                     /note="hexamer: AATAAA"
     polyA_site      9737
                     /gene="HDAC2"
                     /gene_synonym="HD2; KDAC2; RPD3; YAF1"
ORIGIN      
        1 cccctcctcg cgagttggtg ccgctgccac ctccgattcc gagctttcgg cacctctgcc
       61 gggtggtacc gagccttccc ggcgccccct cctctcctcc caccggcctg cccttccccg
      121 cgggactatc gcccccacgt ttccctcagc ccttttctct cccggccgag ccgcggcggc
      181 agcagcagca gcagcagcag caggaggagg agcccggtgg cggcggtggc cggggagccc
      241 atggcgtaca gtcaaggagg cggcaaaaaa aaagtctgct actactacga cggtgatatt
      301 ggaaattatt attatggaca gggtcatccc atgaagcctc atagaatccg catgacccat
      361 aacttgctgt taaattatgg cttatacaga aaaatggaaa tatataggcc ccataaagcc
      421 actgccgaag aaatgacaaa atatcacagt gatgagtata tcaaatttct acggtcaata
      481 agaccagata acatgtctga gtatagtaag cagatgcaga gatttaatgt tggagaagat
      541 tgtccagtgt ttgatggact ctttgagttt tgtcagctct caactggcgg ttcagttgct
      601 ggagctgtga agttaaaccg acaacagact gatatggctg ttaattgggc tggaggatta
      661 catcatgcta agaaatcaga agcatcagga ttctgttacg ttaatgatat tgtgcttgcc
      721 atccttgaat tactaaagta tcatcagaga gtcttatata ttgatataga tattcatcat
      781 ggtgatggtg ttgaagaagc tttttataca acagatcgtg taatgacggt atcattccat
      841 aaatatgggg aatactttcc tggcacagga gacttgaggg atattggtgc tggaaaaggc
      901 aaatactatg ctgtcaattt tccaatgaga gatggtatag atgatgagtc atatgggcag
      961 atatttaagc ctattatctc aaaggtgatg gagatgtatc aacctagtgc tgtggtatta
     1021 cagtgtggtg cagactcatt atctggtgat agactgggtt gtttcaatct aacagtcaaa
     1081 ggtcatgcta aatgtgtaga agttgtaaaa acttttaact taccattact gatgcttgga
     1141 ggaggtggct acacaatccg taatgttgct cgatgttgga catatgagac tgcagttgcc
     1201 cttgattgtg agattcccaa tgagttgcca tataatgatt actttgagta ttttggacca
     1261 gacttcaaac tgcatattag tccttcaaac atgacaaacc agaacactcc agaatatatg
     1321 gaaaagataa aacagcgttt gtttgaaaat ttgcgcatgt tacctcatgc acctggtgtc
     1381 cagatgcaag ctattccaga agatgctgtt catgaagaca gtggagatga agatggagaa
     1441 gatccagaca agagaatttc tattcgagca tcagacaagc ggatagcttg tgatgaagaa
     1501 ttctcagatt ctgaggatga aggagaagga ggtcgaagaa atgtggctga tcataagaaa
     1561 ggagcaaaga aagctagaat tgaagaagat aagaaagaaa cagaggacaa aaaaacagac
     1621 gttaaggaag aagataaatc caaggacaac agtggtgaaa aaacagatac caaaggaacc
     1681 aaatcagaac agctcagcaa cccctgaatt tgacagtctc accaatttca gaaaatcatt
     1741 aaaaagaaaa tattgaaagg aaaatgtttt ctttttgaag acttctggct tcattttata
     1801 ctactttggc atggactgta tttattttca aatggctttt tcgtttttgt ttttcttggc
     1861 aagttttatt gtgagttttt ctaattatga agcaaaattt cttttctcca ccatgcttta
     1921 tgtgatagta tttaaaattg atgtgagtta ttatgtcaaa aaaactgatc tattaaagaa
     1981 gtaattggcc tttctgagct gatttttcca tcttttgtaa ttatctttat taaaaaattg
     2041 tacttggatt atcttttgtc tgtttattac tacaatatga agtcttgttt cagtggctaa
     2101 tgacatcatt tctgtagact tacaatacac tctaggtgaa agataatgat tacagcttga
     2161 aagataacta tttgctgttt ctttgggaag agtatttata gtaattatta cttatctttg
     2221 caatagaaat tctaccacct tgccctctat agcttagcca gttagtatca gtgaagatta
     2281 acatcccatt acaatttatg aaataataca gactctgcaa ttgagatgta ggagttcttt
     2341 gagttgaccc aaagattctc aaaattgaaa tggaaattct gaattgaaag aagaaactga
     2401 ccagaacttg tattgaccag acttgcctat agtatattgc tggtttaaaa tggaacctgc
     2461 agacaaaacc tgtttctttt actgcattta catggcatcc aggttccatt attatttacg
     2521 tgacatccag gtttctaact caaggaaata aacacaactg atttatcatt cagcaactac
     2581 ttattgtgtg tctgcccatt ttaagactgt aggagtgtaa ctgaatataa tggaaaaatg
     2641 ctttcactca gagcttacac tcagagctta cattctagta gcaggaagca gacaaatggg
     2701 ggtaactcct gctactaaga tgtgcaaaga agacaaaaca ttttaggaac ttgccaaaat
     2761 cagtgaaatc tccctttttg tcaggcccac attgattctt ttgagattaa aaattacaga
     2821 atgccagaag ataattcagt caaaagtatt tctcttcagt gcagtaaaat attaaaagaa
     2881 aaaatatttc tctacaagcc tcttaaatgt ttcagacatt cacaatagca cctagacttt
     2941 tgtaatgaac agctgtgcac ccaccccgac ttaacaaata ttttactttt gctatatttg
     3001 cttcggtgtt tttttcttaa aatacaccga aattgaagct ctctttgtgt actttgtcgt
     3061 ttgtaatcct ccctccctcc cagccttaag aagtaatcac cattgtgatt attttatcca
     3121 tgtttttgtg aatattttac ccatgttttt gtacttttgc tacatatttg ttatcaatct
     3181 gtaaaccttt atgacattag gaactaagaa acttagtccc ttcgttaggg ggataatgaa
     3241 atgtatttag tgtttgtgaa acatagatgg tatgtatttg gacaattctg taactttgct
     3301 ttttttattt ttatttttcc atagcttatt ggggaacagg tggtgtttgg ttacatgatt
     3361 aagttcttta gtggtgattt gtgggatttt ggtggaccca tcacccaagc agtgtacact
     3421 gcaccctatt tgtaatcttt tatccctcgc ccccctccca ccatgcctcc cgtctaccat
     3481 gatgatcctg ttttaaataa gaaaatacca tttcgcaggc tccagatgtt ctggcatcct
     3541 ccctgtggat ttcccagtgc ctgcagctca caggacaaca ggggctgtgg tagagtcacc
     3601 tatgagatcc tggagtagtg gatggaggag atggaacagt gaagacggaa actgagctca
     3661 gtatccgggt gccaggagac aaaggccctt tgcttttttt catttaatat tctgatctac
     3721 ccctgttgac acatgttaag tatagttcat tttgactgct atgtattatg ttccattgtg
     3781 tgaacatact gaaattgtac acttcaatac tatactggat ctccttgggt gtatttaaga
     3841 ggttttgttt ttctaagtag ttggttatat acaactaaaa cctcaagaga actatctaaa
     3901 gcaatttcag caaggtgatt tggtacagca ttaataaaca gaaatcagta acacttagtg
     3961 accaagtctg ttggaagaac aaagaccccc atttgtaata acaaaatttt tagaaataat
     4021 atgtaaagaa gctatggttc ttgtgtctag taaggtcaat gtaacatagt aagatgtcag
     4081 aataccctaa tactttaaaa aattcatata ggataaaaat gatatttgaa attggcaagg
     4141 aaagacatta ttttgtaagt ggaattggga caacaactgg taaccaaatg gaaaacccag
     4201 ttttctgccc tccactagaa gatttaaata ggaaaagata aaactaccaa aaacctaaac
     4261 tcttaaagca aatggattga agaaggccta agtgtgacac caaactcaac tataaaagat
     4321 atatttgata acaaaaaaaa aattagttca gtggaccaac aaaaacttag aagacaagtc
     4381 aagaaaaatg acaaaagaca gagtgggagg cagatttgta actcatccag gtcaaaaggc
     4441 tcatatctaa agatagtaga ggaacaaaat gtataaggat gtgaactggg aaacaaatac
     4501 atataaatag tttgtaaata tgaaaagatc tttaacctca gtaaataaaa agctatagag
     4561 agacttattt tttaacttag ttttttaaac ctattatgtt tatttatttt ttcttttttt
     4621 gagacggagt ctcgctgttg cccaggctgg tgtgcaatgg cgcaatctcg gctcactgca
     4681 acctccgcct cccaggttga agccattctc ctgcctcagc ctcctgagta gctgggatta
     4741 caggcgcctg tcaccacgcc cagctaattg ttcgcatttt tagtagagac ggggtttcac
     4801 tatgttggcc aggctggcct cgaactcctg acctcatgat ccacccacct tggccttcca
     4861 aagtgctggg attacaggtg tgagctgccg cacctggctg tttatctctt ttttttagag
     4921 aaaggatctc ggtcacccag gatggagtgt ggtagcctga tcatatctca ctacatctta
     4981 gaacttctag gcttagggta ttctcccacc tcagcctccc aagtagctgg gactacaagt
     5041 gtgcaccatc acacctagct gatttttaca tttttatttt ggagagatgg tgtctccctg
     5101 tgttgcccag gctggtcaca aactcctagg ctcaagcgat tctcctgact caggcatgag
     5161 ccaccgtacc cggcctaaac ctatcatgtt acagacttag aaagcaacta ttgtcaagtg
     5221 tttgaggaaa ctcaggtcag gtttggtaaa ctaagatatt aactcaagta aagctcttta
     5281 attcatttaa tgaaggtgcc acattgtctc agttctctat ggcatgggtg aatgctgttc
     5341 taagtcagca ttggtactct aagctagtta catcatatct aagctttgcc cttctaccag
     5401 agctgctagc attctgtcaa tgggcaatta tttgaagttc ttacattgaa gttagtcacc
     5461 tacatcttct gttttttatg gttttgatgt agtaatactg ctgaagtttt tttgataact
     5521 gcgattcata atatttgtta ttcatattgt gatataaatg ataagggctt ttgaaaacaa
     5581 gtagtaatca tttcaatagc ttaggatctc caccataatc ttaggaaaat tactaacctc
     5641 tgtgcctcag ttgcttcatc atttaaaatg aggaaaataa tagtccctac ttaataggtt
     5701 tgttgtgagg attgagttaa taacatagtt aatgctcagt aagggttagc tgctattttt
     5761 ttttcttttt tttttgaaag agtctcactc tgttgcccag gttggagtgc agtggcatga
     5821 tcttggctca ctgcatcctc tgcttcctag gttcaggtga ttctcatgct tcagcctccc
     5881 aagcagctgg gagtacaggt gtgcaccacc acacctggct aatttttgta tatttagtag
     5941 agacgggttc tcaccatatt gtccaggctg gtcttgaact ccttacctca aatgatccac
     6001 ccgcctgggc ctcccaaagt gctgggatta caagcatgag ccaccgcgcc tggcttgcta
     6061 ttgttatgag gtaaaggtag atagatgggt gagagtggtg ccaggggaag tgttaaattt
     6121 ttgagtgttc ctttagatgc cagatgggtt gtatctgagc cttttattgc agtttgatgc
     6181 ctactagtgt gaagactact aggtcatagt ggatagagaa gcaatctttt ggagacctga
     6241 ttttagcaag gatacgaata atatttgaca actttggggg gatcttgatg cctctgtaat
     6301 ttactcaagg ataatctcaa gaaaaatggc attaagtaga ttacagaaaa aatagaacta
     6361 tcatattgtt attattggct atttacatga gcaatgcgga gaaatgttta ggattacagc
     6421 atttagaagc ttctcaattg ctgcatttcc tcactgtacc acaagatggc agatactgca
     6481 tttaaaattt ttttttctgt gtgttttctc ttatagtcac ttggtggcca tgtaacaagc
     6541 agagcaacat gtattaacag attctttttg aatgcaatat tggattaaaa actttgaatt
     6601 aaactacaat taagtttact gatttaggtt ttcctataag aattataaca ctaatttcta
     6661 agttttacat ctgcaaaaca cagtagagtg tatctggtag tcattgacta attgtgactt
     6721 tgctagtcag acaaaatgga actgaatatt agagccagaa taattttctg ttttgactgg
     6781 tggtaagaat cagttagtat acagaatggg aaagaattga aatttatttc taggaattat
     6841 agttatagtg aagttaatat tcaggggcat tgggtgcttg ttccacgact tttgtttact
     6901 gaactaagta cctaacccct ttggtctttg aacactgcag agttatgatt aggattatta
     6961 atgaaaaatt cttttttagt agaacaaaca gttaaggaca gaagacacct tgattagatt
     7021 ctgttatttg ctctttttat taagattgtt tttactctaa atgttaaaga aatgtataat
     7081 tatttctaag gtaaaatgag tagcaggatt ctgaatagga gatttttttt ggccagaccc
     7141 attttgcagc cagtggtttg tatttaggag tgagttgaac aaagaagact ctaatctgtt
     7201 tttgtgttgt ggatttttta ttctattgat aaatcagtag cttccagctt ctcttgtatc
     7261 ctctactagt catctttttt gtttcttcag aaataaacat ccagggtcac ttccctgccc
     7321 ttgccatata cttactagga tgatgtacag gtaaaacttt taaaggaaag tacggacagg
     7381 caattcctaa agtaaaagag agcacattat caaaactggc tgtacttaac aaataccaac
     7441 aaaaagtcat tacacttctg gcaacaaata atgtttgatt taaaaagaca agaatttccc
     7501 cttttgctct tattttttaa atatgctatg tccttaagtg agaaatgcac ttcaaaaatt
     7561 ttcttattct gaagtctagc cggaaagttt gaacttgtgt tgcctttggc tagactaggt
     7621 gaaattaagg ggcttaagag ggcactgttg tgtggccctg tggtagataa tagccctcca
     7681 agcatgagga tgggagatct accattcacc agtatttcca aagttatatt cttaaaaagg
     7741 ctagatttct aatttccgta gaatctaggt atgttctttt caactcagca tctgataaga
     7801 aagtttgggc aagtaggtgc gaaaaattta ctctagatac cattttccag gaaaaaatat
     7861 aattcaagtc caacgtatta ctagctgtca ccttccccaa caatcaaata agcattgtta
     7921 ttgtcaaaca catttacttg tatagttcag tgttatatgt aatggactta ggtataattc
     7981 ctgtgctatt cccttttgat gccaaacacc cttttgtaag ctgttttgtt tgaaatccaa
     8041 atttatatgt cttaaagtaa atggcagcat tgggctttct taatgtaatg actcttctgc
     8101 catcctattt ctagagttag gcagaaaatt ctgtaaatct atcttatttc cagtctgttt
     8161 gtgactatgg tgggcaataa atctgatttc ccagaaaaac acatctggac ccctataggt
     8221 aaggtaaaca agcctcatct taaattgttt gtacttccga cctgacctca aaacaccatc
     8281 atcatctaga gcagcaacaa gtaaagaaca atgcactgga aatcacatat atgggtctag
     8341 caccagcctt tacattacac aactttgctt tgggcaagtc atttaaattt ctgtgcttca
     8401 gtttactaaa tctatgaaac tgactaggta acagctgaca tgcttttcca gcactccgtc
     8461 ttttacctgt ctgatgtcac aaattgatca ttcctttctg gccttgagat gcgtatcaac
     8521 ccagatagtc attaacccag atagttggca ttcaggataa aatgtttttg cctttcctgt
     8581 cctgcaagct ctttaaagtc caaaatatgc ctttatatct ttatgtgtaa taggtatgaa
     8641 atctatttct tggttgttga ataggatagg cttattagac attctaaagt caactttttt
     8701 ctcatattta aacatttacc cgtagaaatt cagtttttta aagttttaag tcagttgatt
     8761 gtaccaaaca ttactattaa aaaaaaatca aagtcagaga ccagagtttc cctgcatcag
     8821 cgttcagtca ggagacagga actatactaa tttgaacatg taaagttaaa tataatcctt
     8881 aataataaca ggattggaat aagggataaa agaaagagaa ttccttctat atatatgtat
     8941 acacacacat atatatatat acatgtgtat atatgtgtgt gtatgtatat tatataatat
     9001 acatacacac acacaaagaa tgtcagacaa ggagcagcca ccattctgct tactttcact
     9061 ggattaagag tttcataagg gcaagggttt tgtttgtctt acactccact ggggattaat
     9121 ctccagtgtc tagaacagtg cctaacacat aaataaagcc tcaattatta tttgtggaat
     9181 ggaatggaat ttgagtgaaa tggaatggga atagaatgga ctggaatgga aatagaagtg
     9241 ggaaggggaa tggaagggga tagaagagga ttgaagagga cagaaggaga tgggataaga
     9301 tgggatggga taggatcgaa tctcaggtcc aggagtcaga ataactgaat cctattccag
     9361 ttctgccatc aacaagtcct gtgaaatttg gaaagtcact taaccctctg gccttgtaga
     9421 atatctcagt gggcatgtct gatgtttctt catggttaga tataggttgc atgttttggg
     9481 caagaatatc acaggaatga tgctgtgttc tcagtgcacc cagtcaggta atgcatgatt
     9541 tcaatttctc tcattactga tgatgttaac tttaatcact tagtgaatga gaacatgagc
     9601 atcattttga aagcaaactg caaagttttg atgtcaacat tttagcaatt acgtataatt
     9661 tcccccaagg atactggctt tgagataatg taaatataca tgggaaagac aaagctcaaa
     9721 taaaggaaaa agaccca
//
