LOCUS       NM_014266                564 bp    mRNA    linear   PRI 03-MAY-2024
DEFINITION  Homo sapiens hematopoietic cell signal transducer (HCST),
            transcript variant 1, mRNA.
ACCESSION   NM_014266
VERSION     NM_014266.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 564)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   2  (bases 1 to 564)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 564)
  AUTHORS   Qi,X., Qi,C., Wu,T. and Hu,Y.
  TITLE     CSF1R and HCST: Novel Candidate Biomarkers Predicting the Response
            to Immunotherapy in Non-Small Cell Lung Cancer
  JOURNAL   Technol Cancer Res Treat 19, 1533033820970663 (2020)
   PUBMED   33153411
  REMARK    GeneRIF: CSF1R and HCST: Novel Candidate Biomarkers Predicting the
            Response to Immunotherapy in Non-Small Cell Lung Cancer.
REFERENCE   4  (bases 1 to 564)
  AUTHORS   Wilton,K.M., Overlee,B.L. and Billadeau,D.D.
  TITLE     NKG2D-DAP10 signaling recruits EVL to the cytotoxic synapse to
            generate F-actin and promote NK cell cytotoxicity
  JOURNAL   J Cell Sci 133 (5) (2019)
   PUBMED   31235500
  REMARK    GeneRIF: NKG2D-DAP10 signaling recruits EVL to the cytotoxic
            synapse to generate F-actin and promote NK cell cytotoxicity.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 564)
  AUTHORS   Quatrini,L., Molfetta,R., Zitti,B., Peruzzi,G., Fionda,C.,
            Capuano,C., Galandrini,R., Cippitelli,M., Santoni,A. and Paolini,R.
  TITLE     Ubiquitin-dependent endocytosis of NKG2D-DAP10 receptor complexes
            activates signaling and functions in human NK cells
  JOURNAL   Sci Signal 8 (400), ra108 (2015)
   PUBMED   26508790
  REMARK    GeneRIF: These results suggest that NKG2D-DAP10 endocytosis
            represents a means to decrease cell surface receptor abundance, as
            well as to control signaling outcome in cytotoxic lymphocytes.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 564)
  AUTHORS   Yim,D., Jie,H.B., Sotiriadis,J., Kim,Y.S., Kim,K.S.,
            Rothschild,M.F., Lanier,L.L. and Kim,Y.B.
  TITLE     Molecular cloning and characterization of pig immunoreceptor DAP10
            and NKG2D
  JOURNAL   Immunogenetics 53 (3), 243-249 (2001)
   PUBMED   11398969
REFERENCE   7  (bases 1 to 564)
  AUTHORS   Cosman,D., Mullberg,J., Sutherland,C.L., Chin,W., Armitage,R.,
            Fanslow,W., Kubin,M. and Chalupny,N.J.
  TITLE     ULBPs, novel MHC class I-related molecules, bind to CMV
            glycoprotein UL16 and stimulate NK cytotoxicity through the NKG2D
            receptor
  JOURNAL   Immunity 14 (2), 123-133 (2001)
   PUBMED   11239445
REFERENCE   8  (bases 1 to 564)
  AUTHORS   Wu,J., Cherwinski,H., Spies,T., Phillips,J.H. and Lanier,L.L.
  TITLE     DAP10 and DAP12 form distinct, but functionally cooperative,
            receptor complexes in natural killer cells
  JOURNAL   J Exp Med 192 (7), 1059-1068 (2000)
   PUBMED   11015446
REFERENCE   9  (bases 1 to 564)
  AUTHORS   Chang,C., Dietrich,J., Harpur,A.G., Lindquist,J.A., Haude,A.,
            Loke,Y.W., King,A., Colonna,M., Trowsdale,J. and Wilson,M.J.
  TITLE     Cutting edge: KAP10, a novel transmembrane adapter protein
            genetically linked to DAP12 but with unique signaling properties
  JOURNAL   J Immunol 163 (9), 4651-4654 (1999)
   PUBMED   10528161
REFERENCE   10 (bases 1 to 564)
  AUTHORS   Wu,J., Song,Y., Bakker,A.B., Bauer,S., Spies,T., Lanier,L.L. and
            Phillips,J.H.
  TITLE     An activating immunoreceptor complex formed by NKG2D and DAP10
  JOURNAL   Science 285 (5428), 730-732 (1999)
   PUBMED   10426994
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL050163.1 and AD000864.1.
            
            On May 17, 2019 this sequence version replaced NM_014266.3.
            
            Summary: This gene encodes a transmembrane signaling adaptor that
            contains a YxxM motif in its cytoplasmic domain. The encoded
            protein may form part of the immune recognition receptor complex
            with the C-type lectin-like receptor NKG2D. As part of this
            receptor complex, this protein may activate phosphatidylinositol
            3-kinase dependent signaling pathways through its intracytoplasmic
            YxxM motif. This receptor complex may have a role in cell survival
            and proliferation by activation of NK and T cell responses.
            Alternative splicing results in two transcript variants encoding
            different isoforms. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BP226120.1, BC035931.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000246551.9/ ENSP00000246551.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-458               AL050163.1         50-507
            459-564             AD000864.1         38884-38989
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.12"
     gene            1..564
                     /gene="HCST"
                     /gene_synonym="DAP10; KAP10; PIK3AP"
                     /note="hematopoietic cell signal transducer"
                     /db_xref="GeneID:10870"
                     /db_xref="HGNC:HGNC:16977"
                     /db_xref="MIM:604089"
     exon            1..108
                     /gene="HCST"
                     /gene_synonym="DAP10; KAP10; PIK3AP"
                     /inference="alignment:Splign:2.1.0"
     CDS             66..347
                     /gene="HCST"
                     /gene_synonym="DAP10; KAP10; PIK3AP"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; DNAX-activation protein 10;
                     phosphoinositide-3-kinase adaptor protein; kinase assoc
                     pro of ~10kDa; kinase assoc protein; membrane protein
                     DAP10; transmembrane adapter protein KAP10"
                     /codon_start=1
                     /product="hematopoietic cell signal transducer isoform 1
                     precursor"
                     /protein_id="NP_055081.1"
                     /db_xref="CCDS:CCDS32998.1"
                     /db_xref="GeneID:10870"
                     /db_xref="HGNC:HGNC:16977"
                     /db_xref="MIM:604089"
                     /translation="MIHLGHILFLLLLPVAAAQTTPGERSSLPAFYPGTSGSCSGCGS
                     LSLPLLAGLVAADAVASLLIVGAVFLCARPRRSPAQEDGKVYINMPGRG"
     exon            109..174
                     /gene="HCST"
                     /gene_synonym="DAP10; KAP10; PIK3AP"
                     /inference="alignment:Splign:2.1.0"
     exon            175..306
                     /gene="HCST"
                     /gene_synonym="DAP10; KAP10; PIK3AP"
                     /inference="alignment:Splign:2.1.0"
     exon            307..564
                     /gene="HCST"
                     /gene_synonym="DAP10; KAP10; PIK3AP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      439..444
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HCST"
                     /gene_synonym="DAP10; KAP10; PIK3AP"
                     /note="hexamer: AATAAA"
     polyA_site      458
                     /gene="HCST"
                     /gene_synonym="DAP10; KAP10; PIK3AP"
                     /note="major polyA site"
ORIGIN      
        1 acagttcctc tggacttctc tggaccacag tcctctgcca gacccctgcc agaccccagt
       61 ccaccatgat ccatctgggt cacatcctct tcctgctttt gctcccagtg gctgcagctc
      121 agacgactcc aggagagaga tcatcactcc ctgcctttta ccctggcact tcaggctctt
      181 gttccggatg tgggtccctc tctctgccgc tcctggcagg cctcgtggct gctgatgcgg
      241 tggcatcgct gctcatcgtg ggggcggtgt tcctgtgcgc acgcccacgc cgcagccccg
      301 cccaagaaga tggcaaagtc tacatcaaca tgccaggcag gggctgaccc tcctgcagct
      361 tggacctttg acttctgacc ctctcatcct ggatggtgtg tggtggcaca ggaacccccg
      421 ccccaacttt tggattgtaa taaaacaatt gaaacacctg tagtcgtatt ctttctcaaa
      481 gaaccccaga gttcccaaag cctccctccc atgaactgtt tctggatcca aggccccctc
      541 agaaccccca catgtcccca tccc
//
