LOCUS       NM_005477               6922 bp    mRNA    linear   PRI 08-OCT-2024
DEFINITION  Homo sapiens hyperpolarization activated cyclic nucleotide gated
            potassium channel 4 (HCN4), mRNA.
ACCESSION   NM_005477
VERSION     NM_005477.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6922)
  AUTHORS   Fraile,A., Cebrian,J., Thuissard-Vasallo,I., Perez-Martin,S.,
            Casado,R., Gil-Fournier,B., Alonso-Martin,J., Tamargo,J.,
            Caballero,R., Delpon,E. and Cosio,F.G.
  TITLE     Coexistent HCN4 and GATA5 Rare Variants and Atrial Fibrillation in
            a Large Spanish Family
  JOURNAL   Can J Cardiol 40 (7), 1270-1280 (2024)
   PUBMED   38432398
  REMARK    GeneRIF: Coexistent HCN4 and GATA5 Rare Variants and Atrial
            Fibrillation in a Large Spanish Family.
REFERENCE   2  (bases 1 to 6922)
  AUTHORS   Camara-Checa,A., Perin,F., Rubio-Alarcon,M., Dago,M.,
            Crespo-Garcia,T., Rapun,J., Marin,M., Cebrian,J., Gomez,R.,
            Bermudez-Jimenez,F., Monserrat,L., Tamargo,J., Caballero,R.,
            Jimenez-Jaimez,J. and Delpon,E.
  TITLE     A gain-of-function HCN4 mutant in the HCN domain is responsible for
            inappropriate sinus tachycardia in a Spanish family
  JOURNAL   Proc Natl Acad Sci U S A 120 (49), e2305135120 (2023)
   PUBMED   38032931
  REMARK    GeneRIF: A gain-of-function HCN4 mutant in the HCN domain is
            responsible for inappropriate sinus tachycardia in a Spanish
            family.
REFERENCE   3  (bases 1 to 6922)
  AUTHORS   Hsu,Y.H., Pintacuda,G., Liu,R., Nacu,E., Kim,A., Tsafou,K.,
            Petrossian,N., Crotty,W., Suh,J.M., Riseman,J., Martin,J.M.,
            Biagini,J.C., Mena,D., Ching,J.K.T., Malolepsza,E., Li,T.,
            Singh,T., Ge,T., Egri,S.B., Tanenbaum,B., Stanclift,C.R.,
            Apffel,A.M., Carr,S.A., Schenone,M., Jaffe,J., Fornelos,N.,
            Huang,H., Eggan,K.C. and Lage,K.
  CONSRTM   Schizophrenia Working Group of the Psychiatric Genomics Consortium;
            Stanley Global Asia Initiatives
  TITLE     Using brain cell-type-specific protein interactomes to interpret
            neurodevelopmental genetic signals in schizophrenia
  JOURNAL   iScience 26 (5), 106701 (2023)
   PUBMED   37207277
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6922)
  AUTHORS   Wang,H., Wu,T., Huang,Z., Huang,J., Geng,Z., Cui,B., Yan,Y.,
            Zhang,Y. and Wang,Y.
  TITLE     Channel HCN4 mutation R666Q associated with sporadic arrhythmia
            decreases channel electrophysiological function and increases
            protein degradation
  JOURNAL   J Biol Chem 298 (11), 102599 (2022)
   PUBMED   36244448
  REMARK    GeneRIF: Channel HCN4 mutation R666Q associated with sporadic
            arrhythmia decreases channel electrophysiological function and
            increases protein degradation.
REFERENCE   5  (bases 1 to 6922)
  AUTHORS   Qu,J., Altomare,C., Bucchi,A., DiFrancesco,D. and Robinson,R.B.
  TITLE     Functional comparison of HCN isoforms expressed in ventricular and
            HEK 293 cells
  JOURNAL   Pflugers Arch 444 (5), 597-601 (2002)
   PUBMED   12194012
  REMARK    GeneRIF: HCN4 activates substantially slower than HCN2 and with a
            half-maximum activation voltage approximately equal 10 mV less
            negative, both isoforms activate more positively in myocytes
            suggesting cell-type specificity
REFERENCE   6  (bases 1 to 6922)
  AUTHORS   Stevens,D.R., Seifert,R., Bufe,B., Muller,F., Kremmer,E., Gauss,R.,
            Meyerhof,W., Kaupp,U.B. and Lindemann,B.
  TITLE     Hyperpolarization-activated channels HCN1 and HCN4 mediate
            responses to sour stimuli
  JOURNAL   Nature 413 (6856), 631-635 (2001)
   PUBMED   11675786
  REMARK    GeneRIF: The rat HCN1 and HCN4 were shown to mediate responses to
            sour stimuli, suggesting a potential new function for the related
            human proteins.
REFERENCE   7  (bases 1 to 6922)
  AUTHORS   Seifert,R., Scholten,A., Gauss,R., Mincheva,A., Lichter,P. and
            Kaupp,U.B.
  TITLE     Molecular characterization of a slowly gating human
            hyperpolarization-activated channel predominantly expressed in
            thalamus, heart, and testis
  JOURNAL   Proc Natl Acad Sci U S A 96 (16), 9391-9396 (1999)
   PUBMED   10430953
REFERENCE   8  (bases 1 to 6922)
  AUTHORS   Ludwig,A., Zong,X., Stieber,J., Hullin,R., Hofmann,F. and Biel,M.
  TITLE     Two pacemaker channels from human heart with profoundly different
            activation kinetics
  JOURNAL   EMBO J 18 (9), 2323-2329 (1999)
   PUBMED   10228147
REFERENCE   9  (bases 1 to 6922)
  AUTHORS   Brugada,R., Campuzano,O., Sarquella-Brugada,G., Brugada,P.,
            Brugada,J. and Hong,K.
  TITLE     Brugada Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301690
REFERENCE   10 (bases 1 to 6922)
  AUTHORS   Milewicz,D.M. and Cecchi,A.C.
  TITLE     Heritable Thoracic Aortic Disease Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301299
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AJ238850.1, AJ132429.1,
            AC068397.6, BP291686.1 and BM979827.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_005477.2.
            
            Summary: This gene encodes a member of the
            hyperpolarization-activated cyclic nucleotide-gated potassium
            channels. The encoded protein shows slow kinetics of activation and
            inactivation, and is necessary for the cardiac pacemaking process.
            This channel may also mediate responses to sour stimuli. Mutations
            in this gene have been linked to sick sinus syndrome 2, also known
            as atrial fibrillation with bradyarrhythmia or familial sinus
            bradycardia. Two pseudogenes have been identified on chromosome 15.
            [provided by RefSeq, Oct 2008].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AJ132429.1, AJ238850.1 [ECO:0000332]
            RNAseq introns              :: mixed/partial sample support
                                           SAMEA1968968, SAMEA2142348
                                           [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000261917.4/ ENSP00000261917.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-122               AJ238850.1         311-432
            123-5007            AJ132429.1         1-4885
            5008-5008           AC068397.6         130216-130216       c
            5009-5384           BP291686.1         175-550
            5385-6289           AC068397.6         128935-129839       c
            6290-6922           BM979827.1         18-650              c
FEATURES             Location/Qualifiers
     source          1..6922
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q24.1"
     gene            1..6922
                     /gene="HCN4"
                     /gene_synonym="BRGDA8; EIG18; SSS2"
                     /note="hyperpolarization activated cyclic nucleotide gated
                     potassium channel 4"
                     /db_xref="GeneID:10021"
                     /db_xref="HGNC:HGNC:16882"
                     /db_xref="MIM:605206"
     exon            1..1473
                     /gene="HCN4"
                     /gene_synonym="BRGDA8; EIG18; SSS2"
                     /inference="alignment:Splign:2.1.0"
     CDS             689..4300
                     /gene="HCN4"
                     /gene_synonym="BRGDA8; EIG18; SSS2"
                     /note="hyperpolarization activated cyclic nucleotide-gated
                     cation channel 4"
                     /codon_start=1
                     /product="potassium/sodium hyperpolarization-activated
                     cyclic nucleotide-gated channel 4"
                     /protein_id="NP_005468.1"
                     /db_xref="CCDS:CCDS10248.1"
                     /db_xref="GeneID:10021"
                     /db_xref="HGNC:HGNC:16882"
                     /db_xref="MIM:605206"
                     /translation="MDKLPPSMRKRLYSLPQQVGAKAWIMDEEEDAEEEGAGGRQDPS
                     RRSIRLRPLPSPSPSAAAGGTESRSSALGAADSEGPARGAGKSSTNGDCRRFRGSLAS
                     LGSRGGGSGGTGSGSSHGHLHDSAEERRLIAEGDASPGEDRTPPGLAAEPERPGASAQ
                     PAASPPPPQQPPQPASASCEQPSVDTAIKVEGGAAAGDQILPEAEVRLGQAGFMQRQF
                     GAMLQPGVNKFSLRMFGSQKAVEREQERVKSAGFWIIHPYSDFRFYWDLTMLLLMVGN
                     LIIIPVGITFFKDENTTPWIVFNVVSDTFFLIDLVLNFRTGIVVEDNTEIILDPQRIK
                     MKYLKSWFMVDFISSIPVDYIFLIVETRIDSEVYKTARALRIVRFTKILSLLRLLRLS
                     RLIRYIHQWEEIFHMTYDLASAVVRIVNLIGMMLLLCHWDGCLQFLVPMLQDFPDDCW
                     VSINNMVNNSWGKQYSYALFKAMSHMLCIGYGRQAPVGMSDVWLTMLSMIVGATCYAM
                     FIGHATALIQSLDSSRRQYQEKYKQVEQYMSFHKLPPDTRQRIHDYYEHRYQGKMFDE
                     ESILGELSEPLREEIINFNCRKLVASMPLFANADPNFVTSMLTKLRFEVFQPGDYIIR
                     EGTIGKKMYFIQHGVVSVLTKGNKETKLADGSYFGEICLLTRGRRTASVRADTYCRLY
                     SLSVDNFNEVLEEYPMMRRAFETVALDRLDRIGKKNSILLHKVQHDLNSGVFNYQENE
                     IIQQIVQHDREMAHCAHRVQAAASATPTPTPVIWTPLIQAPLQAAAATTSVAIALTHH
                     PRLPAAIFRPPPGSGLGNLGAGQTPRHLKRLQSLIPSALGSASPASSPSQVDTPSSSS
                     FHIQQLAGFSAPAGLSPLLPSSSSSPPPGACGSPSAPTPSAGVAATTIAGFGHFHKAL
                     GGSLSSSDSPLLTPLQPGARSPQAAQPSPAPPGARGGLGLPEHFLPPPPSSRSPSSSP
                     GQLGQPPGELSLGLATGPLSTPETPPRQPEPPSLVAGASGGASPVGFTPRGGLSPPGH
                     SPGPPRTFPSAPPRASGSHGSLLLPPASSPPPPQVPQRRGTPPLTPGRLTQDLKLISA
                     SQPALPQDGAQTLRRASPHSSGESMAAFPLFPRAGGGSGGSGSSGGLGPPGRPYGAIP
                     GQHVTLPRKTSSGSLPPPLSLFGARATSSGGPPLTAGPQREPGARPEPVRSKLPSNL"
     exon            1474..1897
                     /gene="HCN4"
                     /gene_synonym="BRGDA8; EIG18; SSS2"
                     /inference="alignment:Splign:2.1.0"
     exon            1898..2059
                     /gene="HCN4"
                     /gene_synonym="BRGDA8; EIG18; SSS2"
                     /inference="alignment:Splign:2.1.0"
     exon            2060..2278
                     /gene="HCN4"
                     /gene_synonym="BRGDA8; EIG18; SSS2"
                     /inference="alignment:Splign:2.1.0"
     exon            2279..2425
                     /gene="HCN4"
                     /gene_synonym="BRGDA8; EIG18; SSS2"
                     /inference="alignment:Splign:2.1.0"
     exon            2426..2666
                     /gene="HCN4"
                     /gene_synonym="BRGDA8; EIG18; SSS2"
                     /inference="alignment:Splign:2.1.0"
     exon            2667..2831
                     /gene="HCN4"
                     /gene_synonym="BRGDA8; EIG18; SSS2"
                     /inference="alignment:Splign:2.1.0"
     exon            2832..6922
                     /gene="HCN4"
                     /gene_synonym="BRGDA8; EIG18; SSS2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6904..6909
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HCN4"
                     /gene_synonym="BRGDA8; EIG18; SSS2"
                     /note="hexamer: AATAAA"
     polyA_site      6922
                     /gene="HCN4"
                     /gene_synonym="BRGDA8; EIG18; SSS2"
                     /note="major polyA site"
ORIGIN      
        1 gctggagccg ctcctatggc aacccgcgag ctgcggcggc ttcatgaata ttccggggcg
       61 cgggagcccg agcgctgccg gagggcgctt cgggggaggc ggccgctgat gtaagcccgg
      121 cgggtcgctg ggctccgctc ggttgcggcg ggagccccgg gacgggccgg acgggccggg
      181 gcagaggagg cgaggcgagc tcgcgggtgg ccagccacaa agcccgggcg gcgagacaga
      241 cggacagcca gccctcccgc gggacgcacg cccgggaccc gcgcgggccg tgcgctctgc
      301 actccggagc ggttccctga gcgccgcggc cgcagagcct ctccggccgg cgcccattgt
      361 tccccgcggg ggcggggcgc ctggagccgg gcggcgcgcc gcgcccctga acgccagagg
      421 gagggaggga ggcaagaagg gagcgcgggg tccccgcgcc cagccgggcc cgggaggagg
      481 tgtagcgcgg cgagcccggg gactcggagc gggactagga tcctccccgc ggcgcgcagc
      541 ctgcccaagc atgggcgcct gaggctgccc ccacgccggc ggcaaaggac gcgtccccac
      601 gggcggactg accggcgggc ggacctggag cccgtccgcg gcgccgcgct cctgcccccg
      661 gcccggtccg accccggccc ctggcgccat ggacaagctg ccgccgtcca tgcgcaagcg
      721 gctctacagc ctcccgcagc aggtgggggc caaggcgtgg atcatggacg aggaagagga
      781 cgccgaggag gagggggccg ggggccgcca agaccccagc cgcaggagca tccggctgcg
      841 gccactgccc tcgccctccc cctcggcggc cgcgggtggc acggagtccc ggagctcggc
      901 cctcggggca gcggacagcg aagggccggc ccgcggcgcg ggcaagtcca gcacgaacgg
      961 cgactgcagg cgcttccgcg ggagcctggc ctcgctgggc agccggggcg gcggcagcgg
     1021 cggcacgggg agcggcagca gtcacggaca cctgcatgac tccgcggagg agcggcggct
     1081 catcgccgag ggcgacgcgt cccccggcga ggacaggacg cccccaggcc tggcggccga
     1141 gcccgagcgc cccggcgcct cggcgcagcc cgcagcctcg ccgccgccgc cccagcagcc
     1201 accgcagccg gcctccgcct cctgcgagca gccctcggtg gacaccgcta tcaaagtgga
     1261 gggaggcgcg gctgccggcg accagatcct cccggaggcc gaggtgcgcc tgggccaggc
     1321 cggcttcatg cagcgccagt tcggggccat gctccaaccc ggggtcaaca aattctccct
     1381 aaggatgttc ggcagccaga aagccgtgga gcgcgaacag gagagggtca agtcggccgg
     1441 attttggatt atccacccct acagtgactt cagattttac tgggacctga ccatgctgct
     1501 gctgatggtg ggaaacctga ttatcattcc tgtgggcatc accttcttca aggatgagaa
     1561 caccacaccc tggattgtct tcaatgtggt gtcagacaca ttcttcctca tcgacttggt
     1621 cctcaacttc cgcacaggga tcgtggtgga ggacaacaca gagatcatcc tggacccgca
     1681 gcggattaaa atgaagtacc tgaaaagctg gttcatggta gatttcattt cctccatccc
     1741 cgtggactac atcttcctca ttgtggagac acgcatcgac tcggaggtct acaagactgc
     1801 ccgggccctg cgcattgtcc gcttcacgaa gatcctcagc ctcttacgcc tgttacgcct
     1861 ctcccgcctc attcgatata ttcaccagtg ggaagagatc ttccacatga cctacgacct
     1921 ggccagcgcc gtggtgcgca tcgtgaacct catcggcatg atgctcctgc tctgccactg
     1981 ggacggctgc ctgcagttcc tggtacccat gctacaggac ttccctgacg actgctgggt
     2041 gtccatcaac aacatggtga acaactcctg ggggaagcag tactcctacg cgctcttcaa
     2101 ggccatgagc cacatgctgt gcatcggcta cgggcggcag gcgcccgtgg gcatgtccga
     2161 cgtctggctc accatgctca gcatgatcgt gggtgccacc tgctacgcca tgttcattgg
     2221 ccacgccact gccctcatcc agtccctgga ctcctcccgg cgccagtacc aggaaaagta
     2281 caagcaggtg gagcagtaca tgtcctttca caagctcccg cccgacaccc ggcagcgcat
     2341 ccacgactac tacgagcacc gctaccaggg caagatgttc gacgaggaga gcatcctggg
     2401 cgagctaagc gagcccctgc gggaggagat catcaacttt aactgtcgga agctggtggc
     2461 ctccatgcca ctgtttgcca atgcggaccc caacttcgtg acgtccatgc tgaccaagct
     2521 gcgtttcgag gtcttccagc ctggggacta catcatccgg gaaggcacca ttggcaagaa
     2581 gatgtacttc atccagcatg gcgtggtcag cgtgctcacc aagggcaaca aggagaccaa
     2641 gctggccgac ggctcctact ttggagagat ctgcctgctg acccggggcc ggcgcacagc
     2701 cagcgtgagg gccgacacct actgccgcct ctactcgctg agcgtggaca acttcaatga
     2761 ggtgctggag gagtacccca tgatgcgaag ggccttcgag accgtggcgc tggaccgcct
     2821 ggaccgcatt ggcaagaaga actccatcct cctccacaaa gtccagcacg acctcaactc
     2881 cggcgtcttc aactaccagg agaatgagat catccagcag attgtgcagc atgaccggga
     2941 gatggcccac tgcgcgcacc gcgtccaggc tgctgcctct gccaccccaa cccccacgcc
     3001 cgtcatctgg accccgctga tccaggcacc actgcaggct gccgctgcca ccacttctgt
     3061 ggccatagcc ctcacccacc accctcgcct gcctgctgcc atcttccgcc ctcccccagg
     3121 atctgggctg ggcaacctcg gtgccgggca gacgccaagg cacctgaaac ggctgcagtc
     3181 cctgatccct tctgcgctgg gctccgcctc gcccgccagc agcccgtccc aggtggacac
     3241 accgtcttca tcctccttcc acatccaaca gctggctgga ttctctgccc ccgctggact
     3301 gagcccactc ctgccctcat ccagctcctc cccacccccc ggggcctgtg gctccccctc
     3361 ggctcccaca ccatcagctg gcgtagccgc caccaccata gccgggtttg gccacttcca
     3421 caaggcgctg ggtggctccc tgtcctcctc cgactctccc ctgctcaccc cgctgcagcc
     3481 aggcgcccgc tccccgcagg ctgcccagcc atctcccgcg ccacccgggg cccggggagg
     3541 cctgggactc ccggagcact tcctgccacc cccaccctca tccagatccc cgtcatctag
     3601 ccccgggcag ctgggccagc ctcccgggga gttgtcccta ggtctggcca ctggcccact
     3661 gagcacgcca gagacacccc cacggcagcc tgagccgccg tcccttgtgg caggggcctc
     3721 tgggggggct tcccctgtag gctttactcc ccgaggaggt ctcagccccc ctggccacag
     3781 cccaggcccc ccaagaacct tcccgagtgc cccgccccgg gcctctggct cccacggatc
     3841 cttgctcctg ccacctgcat ccagcccccc accaccccag gtcccccagc gccggggcac
     3901 acccccgctc acccccggcc gcctcaccca ggacctcaag ctcatctccg cgtctcagcc
     3961 agccctgcct caggacgggg cgcagactct ccgcagagcc tccccgcact cctcagggga
     4021 gtccatggct gccttcccgc tcttccccag ggctgggggt ggcagcgggg gcagtgggag
     4081 cagcgggggc ctcggtcccc ctgggaggcc ctatggtgcc atccccggcc agcacgtcac
     4141 tctgcctcgg aagacatcct caggttcttt gccaccccct ctgtctttgt ttggggcaag
     4201 agccacctct tctggggggc cccctctgac tgctggaccc cagagggaac ctggggccag
     4261 gcctgagcca gtgcgctcca aactgccatc caatctatga gctgggccct tccttccctc
     4321 ttctttcttc ttttctctcc cttccttctt ccttcaggtt taactgtgat taggagatat
     4381 accaataaca gtaataatta tttaaaaaac cacacacacc agaaaaacaa aagacagcag
     4441 aaaataacca ggtattctta gagctataga tttttggtca cttgctttta tagactattt
     4501 taatactcag cactagaggg agggaggggg agggaggagg gagcaggcag gtcccaaatg
     4561 caaaagccag agaaaggcag atggggtctc cggggctggg caggggtggg agtggccagt
     4621 gttggcggtt cttagagcag atgtgtcatt gtgttcattt agagaaacag ctgccatcag
     4681 cccgttagct gtaacttgga gctccactct gcccccagaa aggggctgcc ctggggtgtg
     4741 ccctggggag cctcagaagc ctgcgacctt gggagaaaag ggccagggcc ctgagggcct
     4801 agcatttttt ctactgtaaa cgtagcaaga tctgtatatg aatatgtata tgtatatgta
     4861 tgtaagatgt gtatatgtat agctatgtag cgctctgtag agccatgtag atagccactc
     4921 acatgtgcgc acacgtgtgc ggtctagttt aatcccatgt tgacaggatg cccaggtcac
     4981 cttacaccca gcaacccgcc ttggcccaca ggctgtgcac tgcatggtct agggacgttc
     5041 tctctccagt cctcagggaa gaggacccca ggacttcgca gcaggccccc tctctcccca
     5101 tctctggtct caaagccagt cccagcctga cctctcacca cacggaagtg gaagactccc
     5161 ctttcctagg gcctcaagca cacaccgcca cctctggggc cgtcagtttg cccatctgta
     5221 cagtgggagg tgagcggaac ttctgtttat tgagtctgct ctgtgccaag cactggtttc
     5281 gcactttaca cacattaact ccttcagttt cacaaagacc atggggtggg tactttgatt
     5341 ctccccattt agcagaggaa gaaacagttt tgggtaattt ttccagaatc atgtaactag
     5401 gagtggcaga gtggggactg atttgaggtt cgagtccacg cctccttgag gcccaagtct
     5461 gtgttccttc catcagaaaa ctgtgttgag gggggctgag gtagatggtc cccaagcatg
     5521 gtacagaagg aagacaccag attttggcag cagtcaggcc tgggtttgaa tcccagccct
     5581 gccacttctt agctgtatga tcttgggcaa gttatctgac ctttctgtca cctcatttgt
     5641 aaaatgggaa taattatggt actgcctcac aaggacctat gaggaccaga tgagaaaaat
     5701 ctatatgtga aatgcccagc ccagcgcctg gcacatacca tggtaggtgc tcaataaaaa
     5761 atcacatttc ttctgcccct catatgccca gcctattgct ccagcaaact atgtgagagc
     5821 ccagggagct ttggctgagg gctccaagac ttaaaatctc aggactcagg aggtggctgg
     5881 gcctccctaa gggcccaagg aaggtgtgtg gccagaggtg ggtgggagcc aggccttgag
     5941 aagtgggaag acttcaacag ggagagaggg agggaatggt gggtgggatg gagtgtatgg
     6001 tggggagatt cctgaggtgg atgtggagtg gtggatcagg gctttgggag gggatcccca
     6061 ggctgagggg tcagagggac ggccttgggt gatagggtaa gggattgtct gggcttagtc
     6121 ctggcaacta ggagccataa gcaggttcca gattgcggga acgagaaagc agctcagatg
     6181 cctttggagg caccatcctc cctcctccca gatgggatct tgccagagcc aaggtcaggg
     6241 gtctgcccct gcctataggg ccagagcagg tatggctgca atccccaagt aatgagaagg
     6301 gctggtccca cattatccat ccagaacctt ccatgctcca agccagaatg ttggcaagat
     6361 cgggttttgc cttgagctat cctgggatgt gagacaaacc gatttctcca tagatgggct
     6421 gcagggagtg ggaggcagta ctccaggaga gaagtgggtg aaggttcctg ggatcttagg
     6481 taaagactag acgccgccta gtactggtct ctactgtgct ggctcaggag ttctgagaac
     6541 tggaaggact tagcctcaac ctgagttctg cacacacccc ttccccttaa ggaaggcagc
     6601 tctgagaggc agcaggactt gatccaaacc cacagtcttg tcctggaggc agcaggggtg
     6661 aaggtggagg gtccagggcc atgaggagcc cccttgccat cagagcctgg cctaaccacc
     6721 ctcttctcta cttacacaca catgcatttt ataatagctc tgacccaacc tggccactct
     6781 gcagagactg ggacagacag gtgcaggcaa tgggccctcc cacacccagt cacctacaag
     6841 gaattttcaa atccactttt aaaacagaaa ccggtaaatg cgccgtattg tatattttat
     6901 ttaaataaaa aaaattccag ca
//
