LOCUS       NM_021072               9933 bp    mRNA    linear   PRI 01-JUL-2024
DEFINITION  Homo sapiens hyperpolarization activated cyclic nucleotide gated
            potassium channel 1 (HCN1), mRNA.
ACCESSION   NM_021072
VERSION     NM_021072.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9933)
  AUTHORS   Burtscher,V., Mount,J., Huang,J., Cowgill,J., Chang,Y., Bickel,K.,
            Chen,J., Yuan,P. and Chanda,B.
  TITLE     Structural basis for hyperpolarization-dependent opening of human
            HCN1 channel
  JOURNAL   Nat Commun 15 (1), 5216 (2024)
   PUBMED   38890331
  REMARK    GeneRIF: Structural basis for hyperpolarization-dependent opening
            of human HCN1 channel.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9933)
  AUTHORS   Yang,Z., Kuang,Z., Liao,H., Gan,S., Peng,X., Yang,H. and Wu,L.
  TITLE     HCN1 pathogenic variants associated with childhood epilepsy in a
            cohort of Chinese patients
  JOURNAL   Epileptic Disord 26 (1), 90-97 (2024)
   PUBMED   38009841
  REMARK    GeneRIF: HCN1 pathogenic variants associated with childhood
            epilepsy in a cohort of Chinese patients.
REFERENCE   3  (bases 1 to 9933)
  AUTHORS   Hsu,Y.H., Pintacuda,G., Liu,R., Nacu,E., Kim,A., Tsafou,K.,
            Petrossian,N., Crotty,W., Suh,J.M., Riseman,J., Martin,J.M.,
            Biagini,J.C., Mena,D., Ching,J.K.T., Malolepsza,E., Li,T.,
            Singh,T., Ge,T., Egri,S.B., Tanenbaum,B., Stanclift,C.R.,
            Apffel,A.M., Carr,S.A., Schenone,M., Jaffe,J., Fornelos,N.,
            Huang,H., Eggan,K.C. and Lage,K.
  CONSRTM   Schizophrenia Working Group of the Psychiatric Genomics Consortium;
            Stanley Global Asia Initiatives
  TITLE     Using brain cell-type-specific protein interactomes to interpret
            neurodevelopmental genetic signals in schizophrenia
  JOURNAL   iScience 26 (5), 106701 (2023)
   PUBMED   37207277
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 9933)
  AUTHORS   Zhao,K., Li,Y., Yang,X. and Zhou,L.
  TITLE     The Impact of Altered HCN1 Expression on Brain Function and Its
            Relationship with Epileptogenesis
  JOURNAL   Curr Neuropharmacol 21 (10), 2070-2078 (2023)
   PUBMED   37366350
  REMARK    GeneRIF: The Impact of Altered HCN1 Expression on Brain Function
            and Its Relationship with Epileptogenesis.
            Review article
REFERENCE   5  (bases 1 to 9933)
  AUTHORS   Schlusche,A.K., Vay,S.U., Kleinenkuhnen,N., Sandke,S.,
            Campos-Martin,R., Florio,M., Huttner,W., Tresch,A., Roeper,J.,
            Rueger,M.A., Jakovcevski,I., Stockebrand,M. and Isbrandt,D.
  TITLE     Developmental HCN channelopathy results in decreased neural
            progenitor proliferation and microcephaly in mice
  JOURNAL   Proc Natl Acad Sci U S A 118 (35) (2021)
   PUBMED   34429357
  REMARK    GeneRIF: Developmental HCN channelopathy results in decreased
            neural progenitor proliferation and microcephaly in mice.
REFERENCE   6  (bases 1 to 9933)
  AUTHORS   Ulens,C. and Tytgat,J.
  TITLE     Functional heteromerization of HCN1 and HCN2 pacemaker channels
  JOURNAL   J Biol Chem 276 (9), 6069-6072 (2001)
   PUBMED   11133998
REFERENCE   7  (bases 1 to 9933)
  AUTHORS   Kleiderlein,J.J., Nisson,P.E., Jessee,J., Li,W.B., Becker,K.G.,
            Derby,M.L., Ross,C.A. and Margolis,R.L.
  TITLE     CCG repeats in cDNAs from human brain
  JOURNAL   Hum Genet 103 (6), 666-673 (1998)
   PUBMED   9921901
  REMARK    Erratum:[Hum Genet 1999 Jan;104(1):113]
REFERENCE   8  (bases 1 to 9933)
  AUTHORS   Ludwig,A., Zong,X., Jeglitsch,M., Hofmann,F. and Biel,M.
  TITLE     A family of hyperpolarization-activated mammalian cation channels
  JOURNAL   Nature 393 (6685), 587-591 (1998)
   PUBMED   9634236
REFERENCE   9  (bases 1 to 9933)
  AUTHORS   Santoro,B., Liu,D.T., Yao,H., Bartsch,D., Kandel,E.R.,
            Siegelbaum,S.A. and Tibbs,G.R.
  TITLE     Identification of a gene encoding a hyperpolarization-activated
            pacemaker channel of brain
  JOURNAL   Cell 93 (5), 717-729 (1998)
   PUBMED   9630217
REFERENCE   10 (bases 1 to 9933)
  AUTHORS   Santoro,B., Grant,S.G., Bartsch,D. and Kandel,E.R.
  TITLE     Interactive cloning with the SH3 domain of N-src identifies a new
            brain specific ion channel protein, with homology to eag and cyclic
            nucleotide-gated channels
  JOURNAL   Proc Natl Acad Sci U S A 94 (26), 14815-14820 (1997)
   PUBMED   9405696
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC114975.2, AF488549.1,
            DR003732.1, AF064876.1, AC099514.2 and CK299850.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_021072.3.
            
            Summary: The membrane protein encoded by this gene is a
            hyperpolarization-activated cation channel that contributes to the
            native pacemaker currents in heart and neurons. The encoded protein
            can homodimerize or heterodimerize with other pore-forming subunits
            to form a potassium channel. This channel may act as a receptor for
            sour tastes. [provided by RefSeq, Oct 2011].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803611.278115.1,
                                           SRR1803611.207132.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1966682,
                                           SAMEA1968189 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000303230.6/ ENSP00000307342.4
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-262               AC114975.2         71064-71325
            263-951             AF488549.1         1-689
            952-1219            DR003732.1         313-580
            1220-2642           AF064876.1         570-1992
            2643-9591           AC099514.2         124557-131505
            9592-9931           CK299850.1         17-356              c
            9932-9933           AC099514.2         131846-131847
FEATURES             Location/Qualifiers
     source          1..9933
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5p12"
     gene            1..9933
                     /gene="HCN1"
                     /gene_synonym="BCNG-1; BCNG1; DEE24; EIEE24; GEFSP10;
                     HAC-2"
                     /note="hyperpolarization activated cyclic nucleotide gated
                     potassium channel 1"
                     /db_xref="GeneID:348980"
                     /db_xref="HGNC:HGNC:4845"
                     /db_xref="MIM:602780"
     exon            1..712
                     /gene="HCN1"
                     /gene_synonym="BCNG-1; BCNG1; DEE24; EIEE24; GEFSP10;
                     HAC-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             288..2960
                     /gene="HCN1"
                     /gene_synonym="BCNG-1; BCNG1; DEE24; EIEE24; GEFSP10;
                     HAC-2"
                     /note="brain cyclic nucleotide-gated channel 1"
                     /codon_start=1
                     /product="potassium/sodium hyperpolarization-activated
                     cyclic nucleotide-gated channel 1"
                     /protein_id="NP_066550.2"
                     /db_xref="CCDS:CCDS3952.1"
                     /db_xref="GeneID:348980"
                     /db_xref="HGNC:HGNC:4845"
                     /db_xref="MIM:602780"
                     /translation="MEGGGKPNSSSNSRDDGNSVFPAKASATGAGPAAAEKRLGTPPG
                     GGGAGAKEHGNSVCFKVDGGGGGGGGGGGGEEPAGGFEDAEGPRRQYGFMQRQFTSML
                     QPGVNKFSLRMFGSQKAVEKEQERVKTAGFWIIHPYSDFRFYWDLIMLIMMVGNLVII
                     PVGITFFTEQTTTPWIIFNVASDTVFLLDLIMNFRTGTVNEDSSEIILDPKVIKMNYL
                     KSWFVVDFISSIPVDYIFLIVEKGMDSEVYKTARALRIVRFTKILSLLRLLRLSRLIR
                     YIHQWEEIFHMTYDLASAVVRIFNLIGMMLLLCHWDGCLQFLVPLLQDFPPDCWVSLN
                     EMVNDSWGKQYSYALFKAMSHMLCIGYGAQAPVSMSDLWITMLSMIVGATCYAMFVGH
                     ATALIQSLDSSRRQYQEKYKQVEQYMSFHKLPADMRQKIHDYYEHRYQGKIFDEENIL
                     NELNDPLREEIVNFNCRKLVATMPLFANADPNFVTAMLSKLRFEVFQPGDYIIREGAV
                     GKKMYFIQHGVAGVITKSSKEMKLTDGSYFGEICLLTKGRRTASVRADTYCRLYSLSV
                     DNFNEVLEEYPMMRRAFETVAIDRLDRIGKKNSILLQKFQKDLNTGVFNNQENEILKQ
                     IVKHDREMVQAIAPINYPQMTTLNSTSSTTTPTSRMRTQSPPVYTATSLSHSNLHSPS
                     PSTQTPQPSAILSPCSYTTAVCSPPVQSPLAARTFHYASPTASQLSLMQQQPQQQVQQ
                     SQPPQTQPQQPSPQPQTPGSSTPKNEVHKSTQALHNTNLTREVRPLSASQPSLPHEVS
                     TLISRPHPTVGESLASIPQPVTAVPGTGLQAGGRSTVPQRVTLFRQMSSGAIPPNRGV
                     PPAPPPPAAALPRESSSVLNTDPDAEKPRFASNL"
     exon            713..1136
                     /gene="HCN1"
                     /gene_synonym="BCNG-1; BCNG1; DEE24; EIEE24; GEFSP10;
                     HAC-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1137..1298
                     /gene="HCN1"
                     /gene_synonym="BCNG-1; BCNG1; DEE24; EIEE24; GEFSP10;
                     HAC-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1299..1517
                     /gene="HCN1"
                     /gene_synonym="BCNG-1; BCNG1; DEE24; EIEE24; GEFSP10;
                     HAC-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1518..1664
                     /gene="HCN1"
                     /gene_synonym="BCNG-1; BCNG1; DEE24; EIEE24; GEFSP10;
                     HAC-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1665..1905
                     /gene="HCN1"
                     /gene_synonym="BCNG-1; BCNG1; DEE24; EIEE24; GEFSP10;
                     HAC-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1906..2070
                     /gene="HCN1"
                     /gene_synonym="BCNG-1; BCNG1; DEE24; EIEE24; GEFSP10;
                     HAC-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2071..9933
                     /gene="HCN1"
                     /gene_synonym="BCNG-1; BCNG1; DEE24; EIEE24; GEFSP10;
                     HAC-2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5607..5612
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HCN1"
                     /gene_synonym="BCNG-1; BCNG1; DEE24; EIEE24; GEFSP10;
                     HAC-2"
                     /note="hexamer: AATAAA"
     regulatory      5616..5621
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HCN1"
                     /gene_synonym="BCNG-1; BCNG1; DEE24; EIEE24; GEFSP10;
                     HAC-2"
                     /note="hexamer: AACAAA"
     polyA_site      5624
                     /gene="HCN1"
                     /gene_synonym="BCNG-1; BCNG1; DEE24; EIEE24; GEFSP10;
                     HAC-2"
                     /note="major polyA site"
     polyA_site      5634
                     /gene="HCN1"
                     /gene_synonym="BCNG-1; BCNG1; DEE24; EIEE24; GEFSP10;
                     HAC-2"
                     /note="major polyA site"
     regulatory      7065..7070
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HCN1"
                     /gene_synonym="BCNG-1; BCNG1; DEE24; EIEE24; GEFSP10;
                     HAC-2"
                     /note="hexamer: AATACA"
     polyA_site      7084
                     /gene="HCN1"
                     /gene_synonym="BCNG-1; BCNG1; DEE24; EIEE24; GEFSP10;
                     HAC-2"
     regulatory      9913..9918
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HCN1"
                     /gene_synonym="BCNG-1; BCNG1; DEE24; EIEE24; GEFSP10;
                     HAC-2"
                     /note="hexamer: AATAAA"
     polyA_site      9933
                     /gene="HCN1"
                     /gene_synonym="BCNG-1; BCNG1; DEE24; EIEE24; GEFSP10;
                     HAC-2"
ORIGIN      
        1 gagccgagct gagcctcgcc tagctccggc agcctcagct tcagcaccag ccgcaccgga
       61 gcccggaccg cagccactaa gggcagcggc ggcggcggga gccaggcgcg cagctagcag
      121 cggcgcggtc gggaagcagc agccgccgcc gccgccgccg ccgccgcgac gggcagctgg
      181 gctcgccggc agccggctcg ggcccctacc ctctcggcta cgtgtctccg gcgccgggcg
      241 gccggcgagt ctggagcccg cgccgtcgcc ggccgcgtcc tccgggcatg gaaggaggcg
      301 gcaagcccaa ctcttcgtct aacagccggg acgatggcaa cagcgtcttc cccgccaagg
      361 cgtccgcgac gggcgcgggg ccggccgcgg ccgagaagcg cctgggcacc ccgccggggg
      421 gcggcggggc cggcgcgaag gagcacggca actccgtgtg cttcaaggtg gacggcggtg
      481 gcggcggtgg cggcggcggc ggcggcggcg aggagccggc ggggggcttc gaagacgccg
      541 aggggccccg gcggcagtac ggcttcatgc agaggcagtt cacctccatg ctgcagcccg
      601 gggtcaacaa attctccctc cgcatgtttg ggagccagaa ggcggtggaa aaggagcagg
      661 aaagggttaa aactgcaggc ttctggatta tccaccctta cagtgatttc aggttttact
      721 gggatttaat aatgcttata atgatggttg gaaatctagt catcatacca gttggaatca
      781 cattctttac agagcaaaca acaacaccat ggattatttt caatgtggca tcagatacag
      841 ttttcctatt ggacctgatc atgaatttta ggactgggac tgtcaatgaa gacagttctg
      901 aaatcatcct ggaccccaaa gtgatcaaga tgaattattt aaaaagctgg tttgtggttg
      961 acttcatctc atccatccca gtggattata tctttcttat tgtagaaaaa ggaatggatt
     1021 ctgaagttta caagacagcc agggcacttc gcattgtgag gtttacaaaa attctcagtc
     1081 tcttgcgttt attacgactt tcaaggttaa ttagatacat acatcaatgg gaagagatat
     1141 tccacatgac atatgatctc gccagtgcag tggtgagaat ttttaatctc atcggcatga
     1201 tgctgctcct gtgccactgg gatggttgtc ttcagttctt agtaccacta ctgcaggact
     1261 tcccaccaga ttgctgggtg tctttaaatg aaatggttaa tgattcttgg ggaaagcagt
     1321 attcatacgc actcttcaaa gctatgagtc acatgctgtg cattgggtat ggagcccaag
     1381 ccccagtcag catgtctgac ctctggatta ccatgctgag catgatcgtc ggggccacct
     1441 gctatgccat gtttgtcggc catgccaccg ctttaatcca gtctctggat tcttcgaggc
     1501 ggcagtatca agagaagtat aagcaagtgg aacaatacat gtcattccat aagttaccag
     1561 ctgatatgcg tcagaagata catgattact atgaacacag ataccaaggc aaaatctttg
     1621 atgaggaaaa tattctcaat gaactcaatg atcctctgag agaggagata gtcaacttca
     1681 actgtcggaa actggtggct acaatgcctt tatttgctaa tgcggatcct aattttgtga
     1741 ctgccatgct gagcaagttg agatttgagg tgtttcaacc tggagattat atcatacgag
     1801 aaggagccgt gggtaaaaaa atgtatttca ttcaacacgg tgttgctggt gtcattacaa
     1861 aatccagtaa agaaatgaag ctgacagatg gctcttactt tggagagatt tgcctgctga
     1921 ccaaaggacg tcgtactgcc agtgttcgag ctgatacata ttgtcgtctt tactcacttt
     1981 ccgtggacaa tttcaacgag gtcctggagg aatatccaat gatgaggaga gcctttgaga
     2041 cagttgccat tgaccgacta gatcgaatag gaaagaaaaa ttcaattctt ctgcaaaagt
     2101 tccagaagga tctgaacact ggtgttttca acaatcagga gaacgaaatc ctcaagcaga
     2161 ttgtgaaaca tgacagggag atggtgcagg caatcgctcc catcaattat cctcaaatga
     2221 caaccctgaa ttccacatcg tctactacga ccccgacctc ccgcatgagg acacaatctc
     2281 caccggtgta cacagcgacc agcctgtctc acagcaacct gcactccccc agtcccagca
     2341 cacagacccc ccagccatca gccatcctgt caccctgctc ctacaccacc gcggtctgca
     2401 gccctcctgt acagagccct ctggccgctc gaactttcca ctatgcctcc cccaccgcct
     2461 cccagctgtc actcatgcaa cagcagccgc agcagcaggt acagcagtcc cagccgccgc
     2521 agactcagcc acagcagccg tccccgcagc cacagacacc tggcagctcc acgccgaaaa
     2581 atgaagtgca caagagcacg caggcgcttc acaacaccaa cctgacccgg gaagtcaggc
     2641 cactctccgc ctcgcagccc tcgctgcccc atgaggtgtc cactctgatt tccagacctc
     2701 atcccactgt gggcgagtcc ctggcctcca tccctcaacc cgtgacggcg gtccccggaa
     2761 cgggccttca ggcagggggc aggagcactg tcccgcagcg cgtcaccctc ttccgacaga
     2821 tgtcgtcggg agccatcccc ccgaaccgag gagtccctcc agcaccccct ccaccagcag
     2881 ctgctcttcc aagagaatct tcctcagtct taaacacaga cccagacgca gaaaagccac
     2941 gatttgcttc aaatttatga tccctgctga ttgtcaaagc agaaagaaat actctcataa
     3001 actgagacta tactcagatc ttattttatt ctatctcctg atagatccct ctagcctact
     3061 atgaagagat attttagaca gctgtggcct acacgtgaaa tgtaaaaata tatatacata
     3121 tactataaaa tatatatcta aattcccaag agagggtcaa aagacctgtt tagcattcag
     3181 tgttatatgt cttcctttct ttaaatcatt aaaggattta aaatgtcgtt gtaagattat
     3241 ttatttctaa cctactttta cttaagtcct ttgatatgta tatttctcta ttttatgaag
     3301 agttcttgga ttcaatggaa acaaaactga ttttaaaaag gcaactcaaa tgaactagta
     3361 aatagcacca atcaaaactt tctttcatta gctgtgtctc tgcatctaaa ttgttaatca
     3421 ttaatggtgg agaattaaat aacaaatccc attttataga tctaaattgt atttcggtgc
     3481 tttcaatttc aaattaggtt aaagaatgca ctacttgctt ggccaccgta ggagactagc
     3541 attgccactg tttgttaaga atatcactaa cctcaaacat gttcattgat ctttcagaaa
     3601 gctgagggaa aattaatatt tgtcttcatg tgttatcgga cttttaccaa gactcgatca
     3661 atgttagttg taaataactt tttcaaccca aataaaaata gctattctgt gttgtatgaa
     3721 ggtaaaagtc attgtttaag aatttagttt tattgcttca cttcaaaact tagagtttta
     3781 aattttacaa aacctgattg tgagaattat tcaattgtaa tgcaatggct tgaaacctac
     3841 aatattattt taacctgcaa tgttttatgc aaaattgtat gctcgaatct acaaattgct
     3901 tgtattacac caaaaatcgt tacttttctt tcttcttgcc ataatcaagc atctgaaaat
     3961 agtccctgca tgcttctggg gaaaaaaaaa taggttcaaa tcagtcattg tagagaaagg
     4021 cttacagtat ttctcgtttc tagaaaagca taacaattca ttaattgcct atcttataat
     4081 tcctataagg ctgacttgga tctctgactt aagtctagaa gtgaggtttt cacttttatt
     4141 taatattatt gctattatta tttaaataat tatttgtaaa ccacagcttg atttggagtt
     4201 aagtgttgtc tgtgtcttca ctgtagctgg taatttacta tggtattaat ttaaagaaat
     4261 aaattcaata cagcatcacg tctgtttggg aacacacatt ttgctctatg tatattgtaa
     4321 ctaaatggtt agtaaacttt aaggcatgtg gtgactaacg tagcccttag ataggaggtt
     4381 tgtcattttt aatgtgttga aaatagtaca gacatggtga cagaaagcag gggtaaagtt
     4441 gaacgtctat gagcattgtc tgtctgtttt cttaactctt tacatgatct gtgagcacct
     4501 gcttcttctc aaagaactcc cttagtaatc atttgtgcct aatgcatccc acacatgtgg
     4561 agtatgactg gaacacaatt gaaagattcc taacaactgg ctgcggtaaa taacccaagt
     4621 taagagcatg acttaaagtg gaggtcttca gtatctctgg cactggcaat acatttaaga
     4681 acatacagag atcacacatt atgtgggaca gggcctgcag atagacttga ggacagaaaa
     4741 caaaaacaaa agcagaaact ctaaagtgga tccttgctgc ctgttatgcc ttttgtggat
     4801 ataataccta taataccaaa atgagttgtt atgtttaatt tcaaacaaat cccaactggt
     4861 gtggaagtaa ttaactgcta gtcgatgtaa tgtaatcatc aatatgctgg tgacatatga
     4921 tggcatgtca ggaatactgt tgtaagaaag ggattgttca gtctacctag aacctatggg
     4981 aaagctgcaa gaccatttac atatcagtac aaaactcctt tattttatta aaatatgaga
     5041 attaacttca atttattaag attacgtatt ggtcctttaa attattaaag gtttacattt
     5101 gataggatta atggatttgg tagtgaaata tttttatata tataaatttt tggtttttta
     5161 ttttgagcac tattgggaaa cataagcaaa attgaaatgc atggtctttc agtgcaacca
     5221 tacaattttt attcactttg taatagaatg gacaaaaaaa ctaaggttta tatattaaac
     5281 taataaaaag gtacagggta aattatatac atatatgtat gaatgtatat atacttagat
     5341 taagaaaaaa aaatgactca cattcctgta atataaaggt ccattgctag ttgaaaagtg
     5401 acctcttttc tctgtacata gtgaagcccg tatgtatgga gttttaatct gtacagaaag
     5461 aaatgtatta ttcattggtc tttctgctgc tggaaagata atagaatagc tgattcttac
     5521 aacaactgta tgatcaggga tgagttagag tatttctttt gtctttgctc aaagccatac
     5581 atatataaat atatataaag attattaata aattcaacaa aaaataaact acaatcatga
     5641 atattttatt ttatcaatat atgctaatca aatgttttat tttattaatg agtacagctt
     5701 tgcctctatt tttacatcat aaaatttcca agagcttata cttgataatc acataaaatc
     5761 aattatattc tataattctg gaatgagagg actttaaata gaacatggat ttttcaacca
     5821 cttctttgtt tacctttttc gtatagtaaa atatgagctt attttctttt ttgctctgtg
     5881 cctttttaga atgtggttgt tctccaactt caaaacacaa ccattttgtg taattattgg
     5941 atatacagat gagaaataat tttcctgtaa taatggtaaa taattgtcct gtaatatatt
     6001 tatacaaacc tattttgtta taatagttct atgacatacc tattttgtta taatagtttt
     6061 ataacatacc tattttgtta taatagggcc ttgggctcta ttataacaaa acaggtttat
     6121 ataaatacat tcatatttat agccttaaac aaaataggtt tgtatagtag gcccaaggcc
     6181 ctattataac aaaataggtt tgtataaata tattcatatt tatagccttg ggccctgcaa
     6241 agttatttgg agagtgatag aacctcccca cacaagtggg ttttaaatga tacctaaaag
     6301 cctgagatag agtttatcat aatgagaata agtagtgaag cattcctgaa aacaaggagg
     6361 ataaatagta gcaatggcaa tatattctat tcaccaaaaa agtagcttgt ctccaggtct
     6421 ggatgacctc ttcataagtg aacaattccc tgatgaatgc tgttccactt taaagagatg
     6481 gttattttgc aatttataaa cttaactgag catactattt tcaggacact acaaagtaca
     6541 tagtcaagtt attaccgtca ttcctatagg acagagaatg atttaaacaa tatgacatgt
     6601 ttaaaatttt agatgattta tttcaaatcc tgaattatgt ctacttcaaa ttatgagcaa
     6661 tctctattat ctttcaggac tatttaaaaa atctccatac ctcctctttc agcttaggtt
     6721 tttatatttg ttaattttac tatgttactt tgcttctatg gccctcactt gcactgttta
     6781 taatgttaac ctgtttgaat gagatatctc tccattatat ctttatcttt ttagaacatg
     6841 tgccataaaa cccatgtggt tattttgttc atatgcacta caactagcaa acttttattt
     6901 tatgttcacc tactttaaaa agtactcata ctgtaccatg ggaaagaaac atgggcctta
     6961 tagaatggaa tgctgagcta ttttgagtcc tgaaaacaat ggccaaaaat ttaaggaact
     7021 gtggttgctg catgcaccaa attgtagcac atacaaataa ataaaataca gttctataac
     7081 tgtattcaat atggttattt tcaaggaaaa ataggataga aattttcttt gaaagatttg
     7141 ctattgtact tagggaagta cacctgctat tacccctcaa aagcaatgac tttcttaagc
     7201 aatttcaatt tttttccttt ttaacaaccc tgcacttgca caaccctgca tctcttgtct
     7261 caccctaatg ctgaccctgc ttccaattca ggctttgaca gggacaaatg gagattttaa
     7321 ggttttttaa tgagagaagc cttgctgaca ctccccgcat cccaccttga aaattattta
     7381 aagatgatct taaaagaacc tacagataat gctctgaatt tgtttacaag gtccttgatg
     7441 tggagagaat gagtaataca ttaaatgctg gggaaataat tatcatttag tgaaaaatta
     7501 agcatgcttt tctaaaatgt ggatgtttaa cagagcacag ctgattggga acactggcag
     7561 ttcaatgcag ctggagtgtc tcacagggtg aagaggaacc cagatcaagt gggaaatagg
     7621 gatttcaaaa accttctgaa aggcttttaa catcagggca aaggttttac agtgttgtac
     7681 tttatattct taatttaagc ttatcaacgg ggaggattca cattgaaatg tctgttttgc
     7741 taatgtaaca aggaataaaa taatttatga cacagcaaaa actgggggct atttacaacc
     7801 ccaccctgcc ttgtgaggat gctgggtcct aaggaaggtc ctaggtttgc taaatagagt
     7861 aatgggaata ggaaactgaa agtttacttg ctttgcgtaa gataatatat tgcggcaggg
     7921 aaggggtaca agaagataag agctagaacc tgtgagaagg gaaagaagtg ataaaataat
     7981 tttggctaaa ataaagcaag ttctcaggcc aggcgtggtg gctcatgcct gtaatcctag
     8041 cactttggga ggccaaagtg ggaggaatgc ttgaggccag gaattggaga ccaaccaggg
     8101 cagcatgtca agacctggtc tctacaagga aaaaaaattt ttttaattga ccagtatata
     8161 cttacatacc tgcagtgttt agaaaacccg actataggtg gtgtattcct gtagtcctag
     8221 ttactcagaa agttaaggca ggaggatcgc ttgaacttag gaggtcaagg ctgcagtgag
     8281 ccctgttcgt tccactgcac tccagcttgg gggacagagc aaaacccttt ctcaagaaaa
     8341 acccaaacaa acaaaaaagt gaagccagtt ctttaatttc tattggagat atcacacacg
     8401 ctccaaagag tggcaaaggt gaacattctt tggagctatt atatttctaa tgtaacatca
     8461 atggccattt actcccaaag ggaaacatct tttttttttt ttttttttaa gatggagtct
     8521 ggctctgtca cccaggtggg agtgcaatgg caggatcttg gcccactgca acatcagcct
     8581 cccaggttta agcaattctc ctgcatcagc ctccagagta gctgggatta caggtgcccg
     8641 ccaccatggc tagctaaatt tttgtaattt ttagtagaga cagggtttta ccatgttggc
     8701 caggccggtc tcgaacccct aacctcaagt gatccgcccg cctcaccctc cagaagtgct
     8761 aggattacag gcctgagcca ccgcacccgg ccctgctatt tttttctatg tagttgtttc
     8821 agtaatacca tatgagcaga gaatactatt gattcattca attgaagaaa tctgaataaa
     8881 ttagtaaata taataattct tcaaaagagg accactttat gtttccaata gattatataa
     8941 ttgaattcag gtaacgtaga gttattcaga cttttttttt taaataacgg aattttgcaa
     9001 aaaaacaggt tgcatcgttt tctacagaga atattcagat gtgtagaggc aaagagactt
     9061 tcttgaaagg tttttatttc ctgttaaatt tgtgaggaaa actcctgaga aaggaaagat
     9121 ttacttttga aggaagaaag aacataaccc aaagaaattt gaaagattca aaaagagttt
     9181 tccaaatgta ttctcatttc tttctcatat ttgttcaagg tcttagtttc caagcattca
     9241 aaattaaatc ttgtttgcct aactcttcta acatctatta ccaacctaac atattacaat
     9301 tctgtttaaa atgtgcagtt ctaaatgttc ttgcgtgcct cactgtggaa agtgtggtgc
     9361 atggaactgc agcgtcagta tcacccagaa gtataattac aagaagtgca gagtccttgg
     9421 ccttaccctg tacctacaga agtccgcttt tgggcaagat ccccagatga ttcatatgtg
     9481 tattaatatt gaaaaaattg gaactagtaa gtcctagagg aggtaaattt taagggcttt
     9541 ttaagggaac ttccaagtgt gaagacattt ctggagatga caagtcaaca tagcatggga
     9601 tcttggctag acaaggaaag cgagagaaca ttaaataaac acaagcaacg acaaaccacc
     9661 tatgttataa ttttatcctg gaccatccaa agtttactct tggtcacgct gtctaaccca
     9721 gcctgcctcc tggcacttgc tagttagaac cagggaagag aaaactaata tgtgttaggt
     9781 attataattt ccttatgtca cataaatttt ggtgccatag ctagcaatgt ttcttaatat
     9841 cacatcagta caatgtgtcc attgccttct gtaacatttt taatgctatc aataatacat
     9901 tgacattata aaaataaatg aatgtggtca ata
//
