LOCUS       NM_005335               1992 bp    mRNA    linear   PRI 12-NOV-2024
DEFINITION  Homo sapiens hematopoietic cell-specific Lyn substrate 1 (HCLS1),
            transcript variant 1, mRNA.
ACCESSION   NM_005335
VERSION     NM_005335.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Li,C., Zhang,L., Zhang,L. and Zhang,G.
  TITLE     Correlation between elevated HCLS1 levels and heart failure: A
            diagnostic biomarker
  JOURNAL   Medicine (Baltimore) 103 (23), e38484 (2024)
   PUBMED   38847679
  REMARK    GeneRIF: Correlation between elevated HCLS1 levels and heart
            failure: A diagnostic biomarker.
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 1992)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1992)
  AUTHORS   Castro-Ochoa,K.F., Guerrero-Fonseca,I.M. and Schnoor,M.
  TITLE     Hematopoietic cell-specific lyn substrate (HCLS1 or HS1): A
            versatile actin-binding protein in leukocytes
  JOURNAL   J Leukoc Biol 105 (5), 881-890 (2019)
   PUBMED   30537294
  REMARK    GeneRIF: Hematopoietic cell-specific Lyn substrate, HCLS1, is a
            versatile actin-binding protein in leukocytes. (Review)
            Review article
REFERENCE   5  (bases 1 to 1992)
  AUTHORS   Hasan,M.K., Yu,J., Chen,L., Cui,B., Widhopf Ii,G.F., Rassenti,L.,
            Shen,Z., Briggs,S.P. and Kipps,T.J.
  TITLE     Wnt5a induces ROR1 to complex with HS1 to enhance migration of
            chronic lymphocytic leukemia cells
  JOURNAL   Leukemia 31 (12), 2615-2622 (2017)
   PUBMED   28465529
  REMARK    GeneRIF: these studies indicate HS1 plays an important role in
            ROR1-dependent Wnt5a-enhanced chemokine-directed leukemia-cell
            migration.
REFERENCE   6  (bases 1 to 1992)
  AUTHORS   Egerton,M., Moritz,R.L., Druker,B., Kelso,A. and Simpson,R.J.
  TITLE     Identification of the 70kD heat shock cognate protein (Hsc70) and
            alpha-actinin-1 as novel phosphotyrosine-containing proteins in T
            lymphocytes
  JOURNAL   Biochem Biophys Res Commun 224 (3), 666-674 (1996)
   PUBMED   8713105
REFERENCE   7  (bases 1 to 1992)
  AUTHORS   Egashira,M., Kitamura,D., Watanabe,T. and Niikawa,N.
  TITLE     The human HCLS1 gene maps to chromosome 3q13 by fluorescence in
            situ hybridization
  JOURNAL   Cytogenet Cell Genet 72 (2-3), 175-176 (1996)
   PUBMED   8978766
REFERENCE   8  (bases 1 to 1992)
  AUTHORS   Hata,D., Nakamura,T., Kawakami,T., Kawakami,Y., Herren,B. and
            Mayumi,M.
  TITLE     Tyrosine phosphorylation of MB-1, B29, and HS1 proteins in human B
            cells following receptor crosslinking
  JOURNAL   Immunol Lett 40 (1), 65-71 (1994)
   PUBMED   7927516
REFERENCE   9  (bases 1 to 1992)
  AUTHORS   Yamanashi,Y., Okada,M., Semba,T., Yamori,T., Umemori,H.,
            Tsunasawa,S., Toyoshima,K., Kitamura,D., Watanabe,T. and
            Yamamoto,T.
  TITLE     Identification of HS1 protein as a major substrate of
            protein-tyrosine kinase(s) upon B-cell antigen receptor-mediated
            signaling
  JOURNAL   Proc Natl Acad Sci U S A 90 (8), 3631-3635 (1993)
   PUBMED   7682714
REFERENCE   10 (bases 1 to 1992)
  AUTHORS   Kitamura,D., Kaneko,H., Miyagoe,Y., Ariyasu,T. and Watanabe,T.
  TITLE     Isolation and characterization of a novel human gene expressed
            specifically in the cells of hematopoietic lineage
  JOURNAL   Nucleic Acids Res 17 (22), 9367-9379 (1989)
   PUBMED   2587259
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC133750.2.
            
            On Nov 23, 2018 this sequence version replaced NM_005335.5.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer protein (isoform 1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1163657.362337.1,
                                           SRR3476690.455893.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000314583.8/ ENSP00000320176.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-84                AC133750.2         32228-32311         c
            85-168              AC133750.2         29672-29755         c
            169-242             AC133750.2         28687-28760         c
            243-372             AC133750.2         18727-18856         c
            373-483             AC133750.2         16226-16336         c
            484-538             AC133750.2         14335-14389         c
            539-649             AC133750.2         8554-8664           c
            650-705             AC133750.2         7842-7897           c
            706-775             AC133750.2         7143-7212           c
            776-987             AC133750.2         5615-5826           c
            988-1092            AC133750.2         4475-4579           c
            1093-1324           AC133750.2         3740-3971           c
            1325-1408           AC133750.2         3509-3592           c
            1409-1992           AC133750.2         2807-3390           c
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q13.33"
     gene            1..1992
                     /gene="HCLS1"
                     /gene_synonym="CTTNL; HS1; lckBP1; p75"
                     /note="hematopoietic cell-specific Lyn substrate 1"
                     /db_xref="GeneID:3059"
                     /db_xref="HGNC:HGNC:4844"
                     /db_xref="MIM:601306"
     exon            1..84
                     /gene="HCLS1"
                     /gene_synonym="CTTNL; HS1; lckBP1; p75"
                     /inference="alignment:Splign:2.1.0"
     CDS             85..1545
                     /gene="HCLS1"
                     /gene_synonym="CTTNL; HS1; lckBP1; p75"
                     /note="isoform 1 is encoded by transcript variant 1;
                     hematopoietic lineage cell-specific protein"
                     /codon_start=1
                     /product="hematopoietic lineage cell-specific protein
                     isoform 1"
                     /protein_id="NP_005326.3"
                     /db_xref="CCDS:CCDS3003.1"
                     /db_xref="GeneID:3059"
                     /db_xref="HGNC:HGNC:4844"
                     /db_xref="MIM:601306"
                     /translation="MWKSVVGHDVSVSVETQGDDWDTDPDFVNDISEKEQRWGAKTIE
                     GSGRTEHINIHQLRNKVSEEHDVLRKKEMESGPKASHGYGGRFGVERDRMDKSAVGHE
                     YVAEVEKHSSQTDAAKGFGGKYGVERDRADKSAVGFDYKGEVEKHTSQKDYSRGFGGR
                     YGVEKDKWDKAALGYDYKGETEKHESQRDYAKGFGGQYGIQKDRVDKSAVGFNEMEAP
                     TTAYKKTTPIEAASSGTRGLKAKFESMAEEKRKREEEEKAQQVARRQQERKAVTKRSP
                     EAPQPVIAMEEPAVPAPLPKKISSEAWPPVGTPPSSESEPVRTSREHPVPLLPIRQTL
                     PEDNEEPPALPPRTLEGLQVEEEPVYEAEPEPEPEPEPEPENDYEDVEEMDRHEQEDE
                     PEGDYEEVLEPEDSSFSSALAGSSGCPAGAGAGAVALGISAVAVYDYQGEGSDELSFD
                     PDDVITDIEMVDEGWWRGRCHGHFGLFPANYVKLLE"
     exon            85..168
                     /gene="HCLS1"
                     /gene_synonym="CTTNL; HS1; lckBP1; p75"
                     /inference="alignment:Splign:2.1.0"
     exon            169..242
                     /gene="HCLS1"
                     /gene_synonym="CTTNL; HS1; lckBP1; p75"
                     /inference="alignment:Splign:2.1.0"
     exon            243..372
                     /gene="HCLS1"
                     /gene_synonym="CTTNL; HS1; lckBP1; p75"
                     /inference="alignment:Splign:2.1.0"
     exon            373..483
                     /gene="HCLS1"
                     /gene_synonym="CTTNL; HS1; lckBP1; p75"
                     /inference="alignment:Splign:2.1.0"
     exon            484..538
                     /gene="HCLS1"
                     /gene_synonym="CTTNL; HS1; lckBP1; p75"
                     /inference="alignment:Splign:2.1.0"
     exon            539..649
                     /gene="HCLS1"
                     /gene_synonym="CTTNL; HS1; lckBP1; p75"
                     /inference="alignment:Splign:2.1.0"
     exon            650..705
                     /gene="HCLS1"
                     /gene_synonym="CTTNL; HS1; lckBP1; p75"
                     /inference="alignment:Splign:2.1.0"
     exon            706..775
                     /gene="HCLS1"
                     /gene_synonym="CTTNL; HS1; lckBP1; p75"
                     /inference="alignment:Splign:2.1.0"
     exon            776..987
                     /gene="HCLS1"
                     /gene_synonym="CTTNL; HS1; lckBP1; p75"
                     /inference="alignment:Splign:2.1.0"
     exon            988..1092
                     /gene="HCLS1"
                     /gene_synonym="CTTNL; HS1; lckBP1; p75"
                     /inference="alignment:Splign:2.1.0"
     exon            1093..1324
                     /gene="HCLS1"
                     /gene_synonym="CTTNL; HS1; lckBP1; p75"
                     /inference="alignment:Splign:2.1.0"
     exon            1325..1408
                     /gene="HCLS1"
                     /gene_synonym="CTTNL; HS1; lckBP1; p75"
                     /inference="alignment:Splign:2.1.0"
     exon            1409..1992
                     /gene="HCLS1"
                     /gene_synonym="CTTNL; HS1; lckBP1; p75"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1965..1970
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HCLS1"
                     /gene_synonym="CTTNL; HS1; lckBP1; p75"
                     /note="hexamer: AATAAA"
     polyA_site      1992
                     /gene="HCLS1"
                     /gene_synonym="CTTNL; HS1; lckBP1; p75"
                     /note="major polyA site"
ORIGIN      
        1 actccgaagt ggcagcagcc agagagggag tcggtgtgga cgcgaggagc cgggcgctta
       61 gaacagaggc ttgcacaggt ggagatgtgg aagtctgtag tgggccatga tgtgtctgtt
      121 tccgtggaga cccagggtga tgattgggac acagatcctg actttgtgaa tgacatctct
      181 gaaaaggagc aacgatgggg agccaagacc atcgaggggt ctggacgcac agaacacatc
      241 aacatccacc agctgaggaa caaagtatca gaggagcatg atgttctcag gaagaaagag
      301 atggagtcag ggcccaaagc atcccatggc tatggaggtc ggtttggagt agaaagagac
      361 cgaatggaca agagtgcagt gggccatgag tatgttgccg aggtggagaa gcactcttct
      421 cagacggatg ctgccaaagg ctttgggggc aagtacggag ttgagaggga cagggcagac
      481 aagtcagcag tcggctttga ttataaagga gaagtggaga agcacacatc tcagaaagat
      541 tactctcgtg gctttggtgg ccggtacggg gtggagaagg ataaatggga caaagcagct
      601 ctgggatatg actacaaggg agagacggag aaacacgagt cccagagaga ttatgccaag
      661 ggctttggtg gccagtatgg aatccagaag gaccgagtgg ataagagcgc tgtcggcttc
      721 aatgaaatgg aggccccgac cacagcttat aagaagacga cgcccataga agccgcttct
      781 agtggtaccc gtgggctgaa ggcgaaattt gagtccatgg ctgaggagaa gaggaagcga
      841 gaggaagagg agaaggcaca gcaggtggcc aggaggcaac aggagcgaaa ggctgtgaca
      901 aagaggagcc ctgaggctcc acagccagtg atagctatgg aagagccagc agtaccggcc
      961 ccactgccca agaaaatctc ctcagaggcc tggcctccag ttgggactcc tccatcatca
     1021 gagtctgagc ctgtgagaac cagcagggaa cacccagtgc ccttgctgcc cattaggcag
     1081 actctcccgg aggacaatga ggagccccca gctctgcccc ctaggactct ggaaggcctc
     1141 caggtggagg aagagccagt gtacgaagca gagcctgagc ctgagcccga gcctgagccc
     1201 gagcctgaga atgactatga ggacgttgag gagatggaca ggcatgagca ggaggatgaa
     1261 ccagaggggg actatgagga ggtgctcgag cctgaagatt cttctttttc ttctgctctg
     1321 gctggatcat caggctgccc ggctggggct ggggctgggg ctgtggctct ggggatctca
     1381 gctgtggctg tatatgatta ccaaggagag ggaagtgatg agctttcctt tgatccggac
     1441 gacgtaatca ctgacattga gatggtggac gagggctggt ggcggggacg ttgccatggc
     1501 cactttggac tcttccctgc aaattatgtc aagcttctgg agtgactaga gctcactgtc
     1561 tactgcaact gtgatttccc atgtccaaag tggctctgcc tccaccccct ccctattcct
     1621 gctgcaaatg tctaaccaga tgaggttctg gacagacttc cctctcctgc ttcattaagg
     1681 gcttggggca gagacagcat ggggaaggag gtccccttcc ccaagagtcc tctctatcct
     1741 ggatgagctc atgaacattt ctcttgtgtt cctgactcct tcccaatgaa cacctctctg
     1801 ccaccccaag ctctgctctc ctcctctgtg agctctgggc ttcccagttt gtttacccgg
     1861 gaaagtacgt ctagattgtg tggtttgcct cattgtgcta tttgcccact ttccttccct
     1921 gaagaaatat ctgaaccttc tttctgttca gtcctaaaat tcgaaataaa gtgagactat
     1981 ggttcacctg ta
//
