LOCUS       NM_002110               2101 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens HCK proto-oncogene, Src family tyrosine kinase (HCK),
            transcript variant 1, mRNA.
ACCESSION   NM_002110
VERSION     NM_002110.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Khella,C.A., Franciosa,L., Rodirguez-Rodriguez,L., Rajkarnikar,R.,
            Mythreye,K. and Gatza,M.L.
  TITLE     HCK Promotes High-Grade Serous Ovarian Cancer Tumorigenesis through
            CD44 and NOTCH3 Signaling
  JOURNAL   Mol Cancer Res 21 (10), 1037-1049 (2023)
   PUBMED   37342066
  REMARK    GeneRIF: HCK Promotes High-Grade Serous Ovarian Cancer
            Tumorigenesis through CD44 and NOTCH3 Signaling.
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Dhami,K., Chakraborty,A., Gururaja,T.L., Cheung,L.W., Sun,C.,
            DeAnda,F. and Huang,X.
  TITLE     Kinase-deficient BTK mutants confer ibrutinib resistance through
            activation of the kinase HCK
  JOURNAL   Sci Signal 15 (736), eabg5216 (2022)
   PUBMED   35639855
  REMARK    GeneRIF: Kinase-deficient BTK mutants confer ibrutinib resistance
            through activation of the kinase HCK.
REFERENCE   3  (bases 1 to 2101)
  AUTHORS   Cheng,F., Li,Q., Wang,J., Wang,L., Li,W. and Zeng,F.
  TITLE     HCK is a Potential Prognostic Biomarker that Correlates with Immune
            Cell Infiltration in Acute Myeloid Leukemia
  JOURNAL   Dis Markers 2022, 3199589 (2022)
   PUBMED   35280440
  REMARK    GeneRIF: HCK is a Potential Prognostic Biomarker that Correlates
            with Immune Cell Infiltration in Acute Myeloid Leukemia.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2101)
  AUTHORS   Carreno,S., Gouze,M.E., Schaak,S., Emorine,L.J. and
            Maridonneau-Parini,I.
  TITLE     Lack of palmitoylation redirects p59Hck from the plasma membrane to
            p61Hck-positive lysosomes
  JOURNAL   J Biol Chem 275 (46), 36223-36229 (2000)
   PUBMED   10967098
  REMARK    GeneRIF: Alternate use of a non-AUG (CUG), and an in-frame,
            downstream AUG translation initiation codon, results in the
            production of two isoforms with different subcellular localization.
REFERENCE   5  (bases 1 to 2101)
  AUTHORS   Robbins,S.M., Quintrell,N.A. and Bishop,J.M.
  TITLE     Myristoylation and differential palmitoylation of the HCK
            protein-tyrosine kinases govern their attachment to membranes and
            association with caveolae
  JOURNAL   Mol Cell Biol 15 (7), 3507-3515 (1995)
   PUBMED   7791757
REFERENCE   6  (bases 1 to 2101)
  AUTHORS   Lichtenberg,U., Quintrell,N. and Bishop,J.M.
  TITLE     Human protein-tyrosine kinase gene HCK: expression and structural
            analysis of the promoter region
  JOURNAL   Oncogene 7 (5), 849-858 (1992)
   PUBMED   1373873
REFERENCE   7  (bases 1 to 2101)
  AUTHORS   Hradetzky,D., Strebhardt,K. and Rubsamen-Waigmann,H.
  TITLE     The genomic locus of the human hemopoietic-specific cell protein
            tyrosine kinase (PTK)-encoding gene (HCK) confirms conservation of
            exon-intron structure among human PTKs of the src family
  JOURNAL   Gene 113 (2), 275-280 (1992)
   PUBMED   1572549
REFERENCE   8  (bases 1 to 2101)
  AUTHORS   Lock,P., Ralph,S., Stanley,E., Boulet,I., Ramsay,R. and Dunn,A.R.
  TITLE     Two isoforms of murine hck, generated by utilization of alternative
            translational initiation codons, exhibit different patterns of
            subcellular localization
  JOURNAL   Mol Cell Biol 11 (9), 4363-4370 (1991)
   PUBMED   1875927
  REMARK    GeneRIF: Alternate use of a non-AUG (CUG), and an in-frame,
            downstream AUG translation initiation codon, results in the
            production of 2 isoforms in mouse and human.
REFERENCE   9  (bases 1 to 2101)
  AUTHORS   Quintrell,N., Lebo,R., Varmus,H., Bishop,J.M., Pettenati,M.J., Le
            Beau,M.M., Diaz,M.O. and Rowley,J.D.
  TITLE     Identification of a human gene (HCK) that encodes a
            protein-tyrosine kinase and is expressed in hemopoietic cells
  JOURNAL   Mol Cell Biol 7 (6), 2267-2275 (1987)
   PUBMED   3496523
REFERENCE   10 (bases 1 to 2101)
  AUTHORS   Ziegler,S.F., Marth,J.D., Lewis,D.B. and Perlmutter,R.M.
  TITLE     Novel protein-tyrosine kinase gene (hck) preferentially expressed
            in cells of hematopoietic origin
  JOURNAL   Mol Cell Biol 7 (6), 2276-2285 (1987)
   PUBMED   3453117
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA465978.1, BC114463.1,
            AW139272.1 and AL049539.21.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Aug 13, 2020 this sequence version replaced NM_002110.4.
            
            Summary: The protein encoded by this gene is a member of the Src
            family of tyrosine kinases. This protein is primarily hemopoietic,
            particularly in cells of the myeloid and B-lymphoid lineages. It
            may help couple the Fc receptor to the activation of the
            respiratory burst. In addition, it may play a role in neutrophil
            migration and in the degranulation of neutrophils. Multiple
            isoforms with different subcellular distributions are produced due
            to both alternative splicing and the use of alternative translation
            initiation codons, including a non-AUG (CUG) codon. [provided by
            RefSeq, Feb 2010].
            
            Transcript Variant: This variant (1) encodes two isoforms due to
            the use of alternative translation initiation codons, as
            demonstrated in PMIDs 1875927 and 7791757. The longer isoform (a,
            also known as p61HCK) is derived from an upstream non-AUG (CUG)
            start codon, while the shorter isoform (b, also known as p59HCK) is
            derived from a downstream AUG start codon. The longer isoform (a)
            is represented in this RefSeq.
            
            CCDS Note: This CCDS, which is supported by the mRNAs AK026432.1,
            BC108930.1 and others, represents a long human HCK isoform, known
            as p61HCK, as described in PMIDs 1875927 and 7791757. This isoform
            initiates translation from a non-AUG (CUG) start codon that is
            well-conserved and present in a strong Kozak signal context.
            Alternative translation initiation from a downstream AUG start
            codon produces an isoform that is 21 aa shorter at the N-terminus.
            The shorter isoform, which is known as p59HCK, is represented by
            CCDS 54455.1. These isoforms exhibit distinct subcellular
            distributions, as indicated in PMID:7791757.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK026432.1, AK225819.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match       :: ENST00000375852.5/ ENSP00000365012.3
            non-AUG initiation codon :: PMID: 7791757, 1875927
            RefSeq Select criteria   :: based on conservation, expression,
                                        longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-19                DA465978.1         55-73
            20-1889             BC114463.1         1-1870
            1890-2088           AW139272.1         27-225              c
            2089-2101           AL049539.21        26404-26416
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20q11.21"
     gene            1..2101
                     /gene="HCK"
                     /gene_synonym="AIPCV; JTK9; p59Hck; p61Hck"
                     /note="HCK proto-oncogene, Src family tyrosine kinase"
                     /db_xref="GeneID:3055"
                     /db_xref="HGNC:HGNC:4840"
                     /db_xref="MIM:142370"
     exon            1..245
                     /gene="HCK"
                     /gene_synonym="AIPCV; JTK9; p59Hck; p61Hck"
                     /inference="alignment:Splign:2.1.0"
     exon            246..366
                     /gene="HCK"
                     /gene_synonym="AIPCV; JTK9; p59Hck; p61Hck"
                     /inference="alignment:Splign:2.1.0"
     exon            367..409
                     /gene="HCK"
                     /gene_synonym="AIPCV; JTK9; p59Hck; p61Hck"
                     /inference="alignment:Splign:2.1.0"
     exon            410..512
                     /gene="HCK"
                     /gene_synonym="AIPCV; JTK9; p59Hck; p61Hck"
                     /inference="alignment:Splign:2.1.0"
     exon            513..611
                     /gene="HCK"
                     /gene_synonym="AIPCV; JTK9; p59Hck; p61Hck"
                     /inference="alignment:Splign:2.1.0"
     exon            612..715
                     /gene="HCK"
                     /gene_synonym="AIPCV; JTK9; p59Hck; p61Hck"
                     /inference="alignment:Splign:2.1.0"
     exon            716..865
                     /gene="HCK"
                     /gene_synonym="AIPCV; JTK9; p59Hck; p61Hck"
                     /inference="alignment:Splign:2.1.0"
     exon            866..1018
                     /gene="HCK"
                     /gene_synonym="AIPCV; JTK9; p59Hck; p61Hck"
                     /inference="alignment:Splign:2.1.0"
     exon            1019..1198
                     /gene="HCK"
                     /gene_synonym="AIPCV; JTK9; p59Hck; p61Hck"
                     /inference="alignment:Splign:2.1.0"
     exon            1199..1275
                     /gene="HCK"
                     /gene_synonym="AIPCV; JTK9; p59Hck; p61Hck"
                     /inference="alignment:Splign:2.1.0"
     exon            1276..1429
                     /gene="HCK"
                     /gene_synonym="AIPCV; JTK9; p59Hck; p61Hck"
                     /inference="alignment:Splign:2.1.0"
     exon            1430..1561
                     /gene="HCK"
                     /gene_synonym="AIPCV; JTK9; p59Hck; p61Hck"
                     /inference="alignment:Splign:2.1.0"
     exon            1562..2101
                     /gene="HCK"
                     /gene_synonym="AIPCV; JTK9; p59Hck; p61Hck"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2067..2072
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HCK"
                     /gene_synonym="AIPCV; JTK9; p59Hck; p61Hck"
                     /note="hexamer: AATAAA"
     regulatory      2076..2081
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HCK"
                     /gene_synonym="AIPCV; JTK9; p59Hck; p61Hck"
                     /note="hexamer: TATAAA"
     polyA_site      2088
                     /gene="HCK"
                     /gene_synonym="AIPCV; JTK9; p59Hck; p61Hck"
                     /note="major polyA site"
     polyA_site      2101
                     /gene="HCK"
                     /gene_synonym="AIPCV; JTK9; p59Hck; p61Hck"
                     /note="major polyA site"
ORIGIN      
        1 accacgtccc tggtcccagc tcgggagcac atcagaggct tagaggcgag tgggaaggga
       61 ctcagacagt gcaggacgag aaacgcccgc ggcaccaaag cccctcagag cgtcgccccc
      121 gcctctagtt ctagaaagtc agtttcccgg cactggcacc ccggaacctc aggggctgcc
      181 gagctggggg ggcgctcaag ctgcgaggat ccgggctgcc cgcgagacga ggagcgggcg
      241 cccaggatgg ggtgcatgaa gtccaagttc ctccaggtcg gaggcaatac attctcaaaa
      301 actgaaacca gcgccagccc acactgtcct gtgtacgtgc cggatcccac atccaccatc
      361 aagccggggc ctaatagcca caacagcaac acaccaggaa tcagggaggc aggctctgag
      421 gacatcatcg tggttgccct gtatgattac gaggccattc accacgaaga cctcagcttc
      481 cagaaggggg accagatggt ggtcctagag gaatccgggg agtggtggaa ggctcgatcc
      541 ctggccaccc ggaaggaggg ctacatccca agcaactatg tcgcccgcgt tgactctctg
      601 gagacagagg agtggttttt caagggcatc agccggaagg acgcagagcg ccaactgctg
      661 gctcccggca acatgctggg ctccttcatg atccgggata gcgagaccac taaaggaagc
      721 tactctttgt ccgtgcgaga ctacgaccct cggcagggag ataccgtgaa acattacaag
      781 atccggaccc tggacaacgg gggcttctac atatcccccc gaagcacctt cagcactctg
      841 caggagctgg tggaccacta caagaagggg aacgacgggc tctgccagaa actgtcggtg
      901 ccctgcatgt cttccaagcc ccagaagcct tgggagaaag atgcctggga gatccctcgg
      961 gaatccctca agctggagaa gaaacttgga gctgggcagt ttggggaagt ctggatggcc
     1021 acctacaaca agcacaccaa ggtggcagtg aagacgatga agccagggag catgtcggtg
     1081 gaggccttcc tggcagaggc caacgtgatg aaaactctgc agcatgacaa gctggtcaaa
     1141 cttcatgcgg tggtcaccaa ggagcccatc tacatcatca cggagttcat ggccaaagga
     1201 agcttgctgg actttctgaa aagtgatgag ggcagcaagc agccattgcc aaaactcatt
     1261 gacttctcag cccagattgc agaaggcatg gccttcatcg agcagaggaa ctacatccac
     1321 cgagacctcc gagctgccaa catcttggtc tctgcatccc tggtgtgtaa gattgctgac
     1381 tttggcctgg cccgggtcat tgaggacaac gagtacacgg ctcgggaagg ggccaagttc
     1441 cccatcaagt ggacagctcc tgaagccatc aactttggct ccttcaccat caagtcagac
     1501 gtctggtcct ttggtatcct gctgatggag atcgtcacct acggccggat cccttaccca
     1561 gggatgtcaa accctgaagt gatccgagct ctggagcgtg gataccggat gcctcgccca
     1621 gagaactgcc cagaggagct ctacaacatc atgatgcgct gctggaaaaa ccgtccggag
     1681 gagcggccga ccttcgaata catccagagt gtgctggatg acttctacac ggccacagag
     1741 agccagtacc aacagcagcc atgataggga ggaccagggc agggccaggg ggtgcccagg
     1801 tggtggctgc aaggtggctc cagcaccatc cgccagggcc cacaccccct tcctactccc
     1861 agacacccac cctcgcttca gccacagttt cctcatctgt ccagtgggta ggttggactg
     1921 gaaaatctct ttttgactct tgcaatccac aatctgacat tctcaggaag cccccaagtt
     1981 gatatttcta tttcctggaa tggttggatt ttagttacag ctgtgatttg gaagggaaac
     2041 tttcaaaata gtgaaatgaa tatttaaata aaagatataa atgccaaagt ctttaccaaa
     2101 a
//
