LOCUS       NM_001172133            2165 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens HCK proto-oncogene, Src family tyrosine kinase (HCK),
            transcript variant 4, mRNA.
ACCESSION   NM_001172133
VERSION     NM_001172133.3
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Khella,C.A., Franciosa,L., Rodirguez-Rodriguez,L., Rajkarnikar,R.,
            Mythreye,K. and Gatza,M.L.
  TITLE     HCK Promotes High-Grade Serous Ovarian Cancer Tumorigenesis through
            CD44 and NOTCH3 Signaling
  JOURNAL   Mol Cancer Res 21 (10), 1037-1049 (2023)
   PUBMED   37342066
  REMARK    GeneRIF: HCK Promotes High-Grade Serous Ovarian Cancer
            Tumorigenesis through CD44 and NOTCH3 Signaling.
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Dhami,K., Chakraborty,A., Gururaja,T.L., Cheung,L.W., Sun,C.,
            DeAnda,F. and Huang,X.
  TITLE     Kinase-deficient BTK mutants confer ibrutinib resistance through
            activation of the kinase HCK
  JOURNAL   Sci Signal 15 (736), eabg5216 (2022)
   PUBMED   35639855
  REMARK    GeneRIF: Kinase-deficient BTK mutants confer ibrutinib resistance
            through activation of the kinase HCK.
REFERENCE   3  (bases 1 to 2165)
  AUTHORS   Cheng,F., Li,Q., Wang,J., Wang,L., Li,W. and Zeng,F.
  TITLE     HCK is a Potential Prognostic Biomarker that Correlates with Immune
            Cell Infiltration in Acute Myeloid Leukemia
  JOURNAL   Dis Markers 2022, 3199589 (2022)
   PUBMED   35280440
  REMARK    GeneRIF: HCK is a Potential Prognostic Biomarker that Correlates
            with Immune Cell Infiltration in Acute Myeloid Leukemia.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2165)
  AUTHORS   Carreno,S., Gouze,M.E., Schaak,S., Emorine,L.J. and
            Maridonneau-Parini,I.
  TITLE     Lack of palmitoylation redirects p59Hck from the plasma membrane to
            p61Hck-positive lysosomes
  JOURNAL   J Biol Chem 275 (46), 36223-36229 (2000)
   PUBMED   10967098
  REMARK    GeneRIF: Alternate use of a non-AUG (CUG), and an in-frame,
            downstream AUG translation initiation codon, results in the
            production of two isoforms with different subcellular localization.
REFERENCE   5  (bases 1 to 2165)
  AUTHORS   Robbins,S.M., Quintrell,N.A. and Bishop,J.M.
  TITLE     Myristoylation and differential palmitoylation of the HCK
            protein-tyrosine kinases govern their attachment to membranes and
            association with caveolae
  JOURNAL   Mol Cell Biol 15 (7), 3507-3515 (1995)
   PUBMED   7791757
REFERENCE   6  (bases 1 to 2165)
  AUTHORS   Lichtenberg,U., Quintrell,N. and Bishop,J.M.
  TITLE     Human protein-tyrosine kinase gene HCK: expression and structural
            analysis of the promoter region
  JOURNAL   Oncogene 7 (5), 849-858 (1992)
   PUBMED   1373873
REFERENCE   7  (bases 1 to 2165)
  AUTHORS   Hradetzky,D., Strebhardt,K. and Rubsamen-Waigmann,H.
  TITLE     The genomic locus of the human hemopoietic-specific cell protein
            tyrosine kinase (PTK)-encoding gene (HCK) confirms conservation of
            exon-intron structure among human PTKs of the src family
  JOURNAL   Gene 113 (2), 275-280 (1992)
   PUBMED   1572549
REFERENCE   8  (bases 1 to 2165)
  AUTHORS   Lock,P., Ralph,S., Stanley,E., Boulet,I., Ramsay,R. and Dunn,A.R.
  TITLE     Two isoforms of murine hck, generated by utilization of alternative
            translational initiation codons, exhibit different patterns of
            subcellular localization
  JOURNAL   Mol Cell Biol 11 (9), 4363-4370 (1991)
   PUBMED   1875927
  REMARK    GeneRIF: Alternate use of a non-AUG (CUG), and an in-frame,
            downstream AUG translation initiation codon, results in the
            production of 2 isoforms in mouse and human.
REFERENCE   9  (bases 1 to 2165)
  AUTHORS   Quintrell,N., Lebo,R., Varmus,H., Bishop,J.M., Pettenati,M.J., Le
            Beau,M.M., Diaz,M.O. and Rowley,J.D.
  TITLE     Identification of a human gene (HCK) that encodes a
            protein-tyrosine kinase and is expressed in hemopoietic cells
  JOURNAL   Mol Cell Biol 7 (6), 2267-2275 (1987)
   PUBMED   3496523
REFERENCE   10 (bases 1 to 2165)
  AUTHORS   Ziegler,S.F., Marth,J.D., Lewis,D.B. and Perlmutter,R.M.
  TITLE     Novel protein-tyrosine kinase gene (hck) preferentially expressed
            in cells of hematopoietic origin
  JOURNAL   Mol Cell Biol 7 (6), 2276-2285 (1987)
   PUBMED   3453117
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK298726.1, AW139272.1 and
            AL049539.21.
            
            On Aug 13, 2020 this sequence version replaced NM_001172133.2.
            
            Summary: The protein encoded by this gene is a member of the Src
            family of tyrosine kinases. This protein is primarily hemopoietic,
            particularly in cells of the myeloid and B-lymphoid lineages. It
            may help couple the Fc receptor to the activation of the
            respiratory burst. In addition, it may play a role in neutrophil
            migration and in the degranulation of neutrophils. Multiple
            isoforms with different subcellular distributions are produced due
            to both alternative splicing and the use of alternative translation
            initiation codons, including a non-AUG (CUG) codon. [provided by
            RefSeq, Feb 2010].
            
            Transcript Variant: This variant (4) differs in its 5' UTR and uses
            the downstream AUG start codon, compared to variant 1, resulting in
            an isoform (b) that is shorter at the N-terminus, compared to
            isoform a. Both variants 1 and 4 encode isoform b.
            
            CCDS Note: This CCDS, which is supported by the mRNAs AK026432.1,
            BC108930.1 and others, represents a short human HCK isoform, known
            as p59HCK, as described in PMID:7791757. This isoform initiates
            translation from a downstream AUG start codon. Alternative
            translation initiation from an upstream non-AUG (CUG) start codon,
            which is well-conserved and present in a strong Kozak signal
            context, produces an isoform that is 21 aa longer at the
            N-terminus. The longer isoform, which is known as p61HCK, is
            represented by CCDS 33460.1. These isoforms exhibit distinct
            subcellular localization, as indicated in PMID:7791757.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK298726.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1969              AK298726.1         1-1969
            1970-2152           AW139272.1         27-209              c
            2153-2165           AL049539.21        26404-26416
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20q11.21"
     gene            1..2165
                     /gene="HCK"
                     /gene_synonym="AIPCV; JTK9; p59Hck; p61Hck"
                     /note="HCK proto-oncogene, Src family tyrosine kinase"
                     /db_xref="GeneID:3055"
                     /db_xref="HGNC:HGNC:4840"
                     /db_xref="MIM:142370"
     exon            1..245
                     /gene="HCK"
                     /gene_synonym="AIPCV; JTK9; p59Hck; p61Hck"
                     /inference="alignment:Splign:2.1.0"
     exon            246..309
                     /gene="HCK"
                     /gene_synonym="AIPCV; JTK9; p59Hck; p61Hck"
                     /inference="alignment:Splign:2.1.0"
     exon            310..430
                     /gene="HCK"
                     /gene_synonym="AIPCV; JTK9; p59Hck; p61Hck"
                     /inference="alignment:Splign:2.1.0"
     CDS             311..1828
                     /gene="HCK"
                     /gene_synonym="AIPCV; JTK9; p59Hck; p61Hck"
                     /EC_number="2.7.10.2"
                     /note="isoform b is encoded by transcript variant 4;
                     p59-HCK/p60-HCK; tyrosine-protein kinase HCK;
                     hematopoietic cell kinase; hemopoietic cell kinase"
                     /codon_start=1
                     /product="tyrosine-protein kinase HCK isoform b"
                     /protein_id="NP_001165604.1"
                     /db_xref="CCDS:CCDS54455.1"
                     /db_xref="GeneID:3055"
                     /db_xref="HGNC:HGNC:4840"
                     /db_xref="MIM:142370"
                     /translation="MGCMKSKFLQVGGNTFSKTETSASPHCPVYVPDPTSTIKPGPNS
                     HNSNTPGIREAGSEDIIVVALYDYEAIHHEDLSFQKGDQMVVLEESGEWWKARSLATR
                     KEGYIPSNYVARVDSLETEEWFFKGISRKDAERQLLAPGNMLGSFMIRDSETTKGSYS
                     LSVRDYDPRQGDTVKHYKIRTLDNGGFYISPRSTFSTLQELVDHYKKGNDGLCQKLSV
                     PCMSSKPQKPWEKDAWEIPRESLKLEKKLGAGQFGEVWMATYNKHTKVAVKTMKPGSM
                     SVEAFLAEANVMKTLQHDKLVKLHAVVTKEPIYIITEFMAKGSLLDFLKSDEGSKQPL
                     PKLIDFSAQIAEGMAFIEQRNYIHRDLRAANILVSASLVCKIADFGLARVIEDNEYTA
                     REGAKFPIKWTAPEAINFGSFTIKSDVWSFGILLMEIVTYGRIPYPGMSNPEVIRALE
                     RGYRMPRPENCPEELYNIMMRCWKNRPEERPTFEYIQSVLDDFYTATESQYQQQP"
     exon            431..473
                     /gene="HCK"
                     /gene_synonym="AIPCV; JTK9; p59Hck; p61Hck"
                     /inference="alignment:Splign:2.1.0"
     exon            474..576
                     /gene="HCK"
                     /gene_synonym="AIPCV; JTK9; p59Hck; p61Hck"
                     /inference="alignment:Splign:2.1.0"
     exon            577..675
                     /gene="HCK"
                     /gene_synonym="AIPCV; JTK9; p59Hck; p61Hck"
                     /inference="alignment:Splign:2.1.0"
     exon            676..779
                     /gene="HCK"
                     /gene_synonym="AIPCV; JTK9; p59Hck; p61Hck"
                     /inference="alignment:Splign:2.1.0"
     exon            780..929
                     /gene="HCK"
                     /gene_synonym="AIPCV; JTK9; p59Hck; p61Hck"
                     /inference="alignment:Splign:2.1.0"
     exon            930..1082
                     /gene="HCK"
                     /gene_synonym="AIPCV; JTK9; p59Hck; p61Hck"
                     /inference="alignment:Splign:2.1.0"
     exon            1083..1262
                     /gene="HCK"
                     /gene_synonym="AIPCV; JTK9; p59Hck; p61Hck"
                     /inference="alignment:Splign:2.1.0"
     exon            1263..1339
                     /gene="HCK"
                     /gene_synonym="AIPCV; JTK9; p59Hck; p61Hck"
                     /inference="alignment:Splign:2.1.0"
     exon            1340..1493
                     /gene="HCK"
                     /gene_synonym="AIPCV; JTK9; p59Hck; p61Hck"
                     /inference="alignment:Splign:2.1.0"
     exon            1494..1625
                     /gene="HCK"
                     /gene_synonym="AIPCV; JTK9; p59Hck; p61Hck"
                     /inference="alignment:Splign:2.1.0"
     exon            1626..2165
                     /gene="HCK"
                     /gene_synonym="AIPCV; JTK9; p59Hck; p61Hck"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2131..2136
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HCK"
                     /gene_synonym="AIPCV; JTK9; p59Hck; p61Hck"
                     /note="hexamer: AATAAA"
     regulatory      2140..2145
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HCK"
                     /gene_synonym="AIPCV; JTK9; p59Hck; p61Hck"
                     /note="hexamer: TATAAA"
     polyA_site      2152
                     /gene="HCK"
                     /gene_synonym="AIPCV; JTK9; p59Hck; p61Hck"
                     /note="major polyA site"
     polyA_site      2165
                     /gene="HCK"
                     /gene_synonym="AIPCV; JTK9; p59Hck; p61Hck"
                     /note="major polyA site"
ORIGIN      
        1 accacgtccc tggtcccagc tcgggagcac atcagaggct tagaggcgag tgggaaggga
       61 ctcagacagt gcaggacgag aaacgcccgc ggcaccaaag cccctcagag cgtcgccccc
      121 gcctctagtt ctagaaagtc agtttcccgg cactggcacc ccggaacctc aggggctgcc
      181 gagctggggg ggcgctcaag ctgcgaggat ccgggctgcc cgcgagacga ggagcgggcg
      241 cccagacaaa caaagcaaaa ccgctcaagt cttgaggtgg caaatctttc ccagttccca
      301 ccagaagagg atggggtgca tgaagtccaa gttcctccag gtcggaggca atacattctc
      361 aaaaactgaa accagcgcca gcccacactg tcctgtgtac gtgccggatc ccacatccac
      421 catcaagccg gggcctaata gccacaacag caacacacca ggaatcaggg aggcaggctc
      481 tgaggacatc atcgtggttg ccctgtatga ttacgaggcc attcaccacg aagacctcag
      541 cttccagaag ggggaccaga tggtggtcct agaggaatcc ggggagtggt ggaaggctcg
      601 atccctggcc acccggaagg agggctacat cccaagcaac tatgtcgccc gcgttgactc
      661 tctggagaca gaggagtggt ttttcaaggg catcagccgg aaggacgcag agcgccaact
      721 gctggctccc ggcaacatgc tgggctcctt catgatccgg gatagcgaga ccactaaagg
      781 aagctactct ttgtccgtgc gagactacga ccctcggcag ggagataccg tgaaacatta
      841 caagatccgg accctggaca acgggggctt ctacatatcc ccccgaagca ccttcagcac
      901 tctgcaggag ctggtggacc actacaagaa ggggaacgac gggctctgcc agaaactgtc
      961 ggtgccctgc atgtcttcca agccccagaa gccttgggag aaagatgcct gggagatccc
     1021 tcgggaatcc ctcaagctgg agaagaaact tggagctggg cagtttgggg aagtctggat
     1081 ggccacctac aacaagcaca ccaaggtggc agtgaagacg atgaagccag ggagcatgtc
     1141 ggtggaggcc ttcctggcag aggccaacgt gatgaaaact ctgcagcatg acaagctggt
     1201 caaacttcat gcggtggtca ccaaggagcc catctacatc atcacggagt tcatggccaa
     1261 aggaagcttg ctggactttc tgaaaagtga tgagggcagc aagcagccat tgccaaaact
     1321 cattgacttc tcagcccaga ttgcagaagg catggccttc atcgagcaga ggaactacat
     1381 ccaccgagac ctccgagctg ccaacatctt ggtctctgca tccctggtgt gtaagattgc
     1441 tgactttggc ctggcccggg tcattgagga caacgagtac acggctcggg aaggggccaa
     1501 gttccccatc aagtggacag ctcctgaagc catcaacttt ggctccttca ccatcaagtc
     1561 agacgtctgg tcctttggta tcctgctgat ggagatcgtc acctacggcc ggatccctta
     1621 cccagggatg tcaaaccctg aagtgatccg agctctggag cgtggatacc ggatgcctcg
     1681 cccagagaac tgcccagagg agctctacaa catcatgatg cgctgctgga aaaaccgtcc
     1741 ggaggagcgg ccgaccttcg aatacatcca gagtgtgctg gatgacttct acacggccac
     1801 agagagccag taccaacagc agccatgata gggaggacca gggcagggcc agggggtgcc
     1861 caggtggtgg ctgcaaggtg gctccagcac catccgccag ggcccacacc cccttcctac
     1921 tcccagacac ccaccctcgc ttcagccaca gtttcctcat ctgtccagtg ggtaggttgg
     1981 actggaaaat ctctttttga ctcttgcaat ccacaatctg acattctcag gaagccccca
     2041 agttgatatt tctatttcct ggaatggttg gattttagtt acagctgtga tttggaaggg
     2101 aaactttcaa aatagtgaaa tgaatattta aataaaagat ataaatgcca aagtctttac
     2161 caaaa
//
