LOCUS       NM_006620               7087 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens HBS1 like translational GTPase (HBS1L), transcript
            variant 1, mRNA.
ACCESSION   NM_006620
VERSION     NM_006620.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7087)
  AUTHORS   Chumchuen,S., Sripichai,O., Jearawiriyapaisarn,N., Fucharoen,S. and
            Peerapittayamongkol,C.
  TITLE     Induction of fetal hemoglobin: Lentiviral shRNA knockdown of HBS1L
            in beta0-thalassemia/HbE erythroid cells
  JOURNAL   PLoS One 18 (3), e0281059 (2023)
   PUBMED   36888630
  REMARK    GeneRIF: Induction of fetal hemoglobin: Lentiviral shRNA knockdown
            of HBS1L in beta0-thalassemia/HbE erythroid cells.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7087)
  AUTHORS   Ganel,L., Chen,L., Christ,R., Vangipurapu,J., Young,E., Das,I.,
            Kanchi,K., Larson,D., Regier,A., Abel,H., Kang,C.J., Scott,A.,
            Havulinna,A., Chiang,C.W.K., Service,S., Freimer,N., Palotie,A.,
            Ripatti,S., Kuusisto,J., Boehnke,M., Laakso,M., Locke,A.,
            Stitziel,N.O. and Hall,I.M.
  TITLE     Mitochondrial genome copy number measured by DNA sequencing in
            human blood is strongly associated with metabolic traits via
            cell-type composition differences
  JOURNAL   Hum Genomics 15 (1), 34 (2021)
   PUBMED   34099068
  REMARK    GeneRIF: Mitochondrial genome copy number measured by DNA
            sequencing in human blood is strongly associated with metabolic
            traits via cell-type composition differences.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7087)
  AUTHORS   Sales,R.R., Belisario,A.R., Faria,G., Mendes,F., Luizon,M.R. and
            Viana,M.B.
  TITLE     Functional polymorphisms of BCL11A and HBS1L-MYB genes affect both
            fetal hemoglobin level and clinical outcomes in a cohort of
            children with sickle cell anemia
  JOURNAL   Ann Hematol 99 (7), 1453-1463 (2020)
   PUBMED   32447424
  REMARK    GeneRIF: Patients carrying Fetal Hemoglobin-boosting alleles of
            BCL11A and HMIP-2 ( (HBS1L-MYB) were associated with milder
            clinical phenotypes. Higher HbF concentration may underlie this
            effect.
REFERENCE   4  (bases 1 to 7087)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 7087)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 7087)
  AUTHORS   Lettre,G., Sankaran,V.G., Bezerra,M.A., Araujo,A.S., Uda,M.,
            Sanna,S., Cao,A., Schlessinger,D., Costa,F.F., Hirschhorn,J.N. and
            Orkin,S.H.
  TITLE     DNA polymorphisms at the BCL11A, HBS1L-MYB, and beta-globin loci
            associate with fetal hemoglobin levels and pain crises in sickle
            cell disease
  JOURNAL   Proc Natl Acad Sci U S A 105 (33), 11869-11874 (2008)
   PUBMED   18667698
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   7  (bases 1 to 7087)
  AUTHORS   Menzel,S., Jiang,J., Silver,N., Gallagher,J., Cunningham,J.,
            Surdulescu,G., Lathrop,M., Farrall,M., Spector,T.D. and Thein,S.L.
  TITLE     The HBS1L-MYB intergenic region on chromosome 6q23.3 influences
            erythrocyte, platelet, and monocyte counts in humans
  JOURNAL   Blood 110 (10), 3624-3626 (2007)
   PUBMED   17712044
  REMARK    GeneRIF: The HBS1L-MYB intergenic region on chromosome 6q23.3
            influences erythrocyte, platelet, and monocyte counts.
REFERENCE   8  (bases 1 to 7087)
  AUTHORS   Thein,S.L., Menzel,S., Peng,X., Best,S., Jiang,J., Close,J.,
            Silver,N., Gerovasilli,A., Ping,C., Yamaguchi,M., Wahlberg,K.,
            Ulug,P., Spector,T.D., Garner,C., Matsuda,F., Farrall,M. and
            Lathrop,M.
  TITLE     Intergenic variants of HBS1L-MYB are responsible for a major
            quantitative trait locus on chromosome 6q23 influencing fetal
            hemoglobin levels in adults
  JOURNAL   Proc Natl Acad Sci U S A 104 (27), 11346-11351 (2007)
   PUBMED   17592125
  REMARK    GeneRIF: HBS1L-related genetic variants play a key role in control
            of fetal hemoglobin levels.
REFERENCE   9  (bases 1 to 7087)
  AUTHORS   Mungall,A.J., Palmer,S.A., Sims,S.K., Edwards,C.A., Ashurst,J.L.,
            Wilming,L., Jones,M.C., Horton,R., Hunt,S.E., Scott,C.E.,
            Gilbert,J.G., Clamp,M.E., Bethel,G., Milne,S., Ainscough,R.,
            Almeida,J.P., Ambrose,K.D., Andrews,T.D., Ashwell,R.I.,
            Babbage,A.K., Bagguley,C.L., Bailey,J., Banerjee,R., Barker,D.J.,
            Barlow,K.F., Bates,K., Beare,D.M., Beasley,H., Beasley,O.,
            Bird,C.P., Blakey,S., Bray-Allen,S., Brook,J., Brown,A.J.,
            Brown,J.Y., Burford,D.C., Burrill,W., Burton,J., Carder,C.,
            Carter,N.P., Chapman,J.C., Clark,S.Y., Clark,G., Clee,C.M.,
            Clegg,S., Cobley,V., Collier,R.E., Collins,J.E., Colman,L.K.,
            Corby,N.R., Coville,G.J., Culley,K.M., Dhami,P., Davies,J.,
            Dunn,M., Earthrowl,M.E., Ellington,A.E., Evans,K.A., Faulkner,L.,
            Francis,M.D., Frankish,A., Frankland,J., French,L., Garner,P.,
            Garnett,J., Ghori,M.J., Gilby,L.M., Gillson,C.J., Glithero,R.J.,
            Grafham,D.V., Grant,M., Gribble,S., Griffiths,C., Griffiths,M.,
            Hall,R., Halls,K.S., Hammond,S., Harley,J.L., Hart,E.A.,
            Heath,P.D., Heathcott,R., Holmes,S.J., Howden,P.J., Howe,K.L.,
            Howell,G.R., Huckle,E., Humphray,S.J., Humphries,M.D., Hunt,A.R.,
            Johnson,C.M., Joy,A.A., Kay,M., Keenan,S.J., Kimberley,A.M.,
            King,A., Laird,G.K., Langford,C., Lawlor,S., Leongamornlert,D.A.,
            Leversha,M., Lloyd,C.R., Lloyd,D.M., Loveland,J.E., Lovell,J.,
            Martin,S., Mashreghi-Mohammadi,M., Maslen,G.L., Matthews,L.,
            McCann,O.T., McLaren,S.J., McLay,K., McMurray,A., Moore,M.J.,
            Mullikin,J.C., Niblett,D., Nickerson,T., Novik,K.L., Oliver,K.,
            Overton-Larty,E.K., Parker,A., Patel,R., Pearce,A.V., Peck,A.I.,
            Phillimore,B., Phillips,S., Plumb,R.W., Porter,K.M., Ramsey,Y.,
            Ranby,S.A., Rice,C.M., Ross,M.T., Searle,S.M., Sehra,H.K.,
            Sheridan,E., Skuce,C.D., Smith,S., Smith,M., Spraggon,L.,
            Squares,S.L., Steward,C.A., Sycamore,N., Tamlyn-Hall,G., Tester,J.,
            Theaker,A.J., Thomas,D.W., Thorpe,A., Tracey,A., Tromans,A.,
            Tubby,B., Wall,M., Wallis,J.M., West,A.P., White,S.S.,
            Whitehead,S.L., Whittaker,H., Wild,A., Willey,D.J., Wilmer,T.E.,
            Wood,J.M., Wray,P.W., Wyatt,J.C., Young,L., Younger,R.M.,
            Bentley,D.R., Coulson,A., Durbin,R., Hubbard,T., Sulston,J.E.,
            Dunham,I., Rogers,J. and Beck,S.
  TITLE     The DNA sequence and analysis of human chromosome 6
  JOURNAL   Nature 425 (6960), 805-811 (2003)
   PUBMED   14574404
REFERENCE   10 (bases 1 to 7087)
  AUTHORS   Wallrapp,C., Verrier,S.B., Zhouravleva,G., Philippe,H.,
            Philippe,M., Gress,T.M. and Jean-Jean,O.
  TITLE     The product of the mammalian orthologue of the Saccharomyces
            cerevisiae HBS1 gene is phylogenetically related to eukaryotic
            release factor 3 (eRF3) but does not carry eRF3-like activity
  JOURNAL   FEBS Lett 440 (3), 387-392 (1998)
   PUBMED   9872408
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB051030.1, BC001465.2 and
            AL445190.9.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_006620.3.
            
            Summary: This gene encodes a member of the GTP-binding elongation
            factor family. It is expressed in multiple tissues with the highest
            expression in heart and skeletal muscle. The intergenic region of
            this gene and the MYB gene has been identified to be a quantitative
            trait locus (QTL) controlling fetal hemoglobin level, and this
            region influnces erythrocyte, platelet, and monocyte counts as well
            as erythrocyte volume and hemoglobin content. DNA polymorphisms at
            this region associate with fetal hemoglobin levels and pain crises
            in sickle cell disease. A single nucleotide polymorphism in exon 1
            of this gene is significantly associated with severity in
            beta-thalassemia/Hemoglobin E. Multiple alternatively spliced
            transcript variants encoding different protein isoforms have been
            found for this gene. [provided by RefSeq, May 2009].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AJ459826.1, SRR1660803.140634.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000367837.10/ ENSP00000356811.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-15                DB051030.1         77-91
            16-2331             BC001465.2         1-2316
            2332-7087           AL445190.9         21070-25825         c
FEATURES             Location/Qualifiers
     source          1..7087
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q23.3"
     gene            1..7087
                     /gene="HBS1L"
                     /gene_synonym="EF-1a; eRF3c; ERFS; HBS1; HSPC276"
                     /note="HBS1 like translational GTPase"
                     /db_xref="GeneID:10767"
                     /db_xref="HGNC:HGNC:4834"
                     /db_xref="MIM:612450"
     exon            1..174
                     /gene="HBS1L"
                     /gene_synonym="EF-1a; eRF3c; ERFS; HBS1; HSPC276"
                     /inference="alignment:Splign:2.1.0"
     exon            175..240
                     /gene="HBS1L"
                     /gene_synonym="EF-1a; eRF3c; ERFS; HBS1; HSPC276"
                     /inference="alignment:Splign:2.1.0"
     exon            241..366
                     /gene="HBS1L"
                     /gene_synonym="EF-1a; eRF3c; ERFS; HBS1; HSPC276"
                     /inference="alignment:Splign:2.1.0"
     exon            367..561
                     /gene="HBS1L"
                     /gene_synonym="EF-1a; eRF3c; ERFS; HBS1; HSPC276"
                     /inference="alignment:Splign:2.1.0"
     exon            562..670
                     /gene="HBS1L"
                     /gene_synonym="EF-1a; eRF3c; ERFS; HBS1; HSPC276"
                     /inference="alignment:Splign:2.1.0"
     exon            671..930
                     /gene="HBS1L"
                     /gene_synonym="EF-1a; eRF3c; ERFS; HBS1; HSPC276"
                     /inference="alignment:Splign:2.1.0"
     exon            931..1096
                     /gene="HBS1L"
                     /gene_synonym="EF-1a; eRF3c; ERFS; HBS1; HSPC276"
                     /inference="alignment:Splign:2.1.0"
     exon            1097..1214
                     /gene="HBS1L"
                     /gene_synonym="EF-1a; eRF3c; ERFS; HBS1; HSPC276"
                     /inference="alignment:Splign:2.1.0"
     exon            1215..1361
                     /gene="HBS1L"
                     /gene_synonym="EF-1a; eRF3c; ERFS; HBS1; HSPC276"
                     /inference="alignment:Splign:2.1.0"
     exon            1362..1436
                     /gene="HBS1L"
                     /gene_synonym="EF-1a; eRF3c; ERFS; HBS1; HSPC276"
                     /inference="alignment:Splign:2.1.0"
     exon            1437..1554
                     /gene="HBS1L"
                     /gene_synonym="EF-1a; eRF3c; ERFS; HBS1; HSPC276"
                     /inference="alignment:Splign:2.1.0"
     exon            1555..1623
                     /gene="HBS1L"
                     /gene_synonym="EF-1a; eRF3c; ERFS; HBS1; HSPC276"
                     /inference="alignment:Splign:2.1.0"
     exon            1624..1728
                     /gene="HBS1L"
                     /gene_synonym="EF-1a; eRF3c; ERFS; HBS1; HSPC276"
                     /inference="alignment:Splign:2.1.0"
     exon            1729..1819
                     /gene="HBS1L"
                     /gene_synonym="EF-1a; eRF3c; ERFS; HBS1; HSPC276"
                     /inference="alignment:Splign:2.1.0"
     exon            1820..1928
                     /gene="HBS1L"
                     /gene_synonym="EF-1a; eRF3c; ERFS; HBS1; HSPC276"
                     /inference="alignment:Splign:2.1.0"
     exon            1929..2029
                     /gene="HBS1L"
                     /gene_synonym="EF-1a; eRF3c; ERFS; HBS1; HSPC276"
                     /inference="alignment:Splign:2.1.0"
     exon            2030..2174
                     /gene="HBS1L"
                     /gene_synonym="EF-1a; eRF3c; ERFS; HBS1; HSPC276"
                     /inference="alignment:Splign:2.1.0"
     exon            2175..7087
                     /gene="HBS1L"
                     /gene_synonym="EF-1a; eRF3c; ERFS; HBS1; HSPC276"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2809..2814
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HBS1L"
                     /gene_synonym="EF-1a; eRF3c; ERFS; HBS1; HSPC276"
                     /note="hexamer: AATAAA"
     polyA_site      2834
                     /gene="HBS1L"
                     /gene_synonym="EF-1a; eRF3c; ERFS; HBS1; HSPC276"
                     /note="major polyA site"
     regulatory      3465..3470
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HBS1L"
                     /gene_synonym="EF-1a; eRF3c; ERFS; HBS1; HSPC276"
                     /note="hexamer: AATACA"
     polyA_site      3488
                     /gene="HBS1L"
                     /gene_synonym="EF-1a; eRF3c; ERFS; HBS1; HSPC276"
     regulatory      5049..5054
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HBS1L"
                     /gene_synonym="EF-1a; eRF3c; ERFS; HBS1; HSPC276"
                     /note="hexamer: AATAAA"
     polyA_site      5080
                     /gene="HBS1L"
                     /gene_synonym="EF-1a; eRF3c; ERFS; HBS1; HSPC276"
     regulatory      6378..6383
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HBS1L"
                     /gene_synonym="EF-1a; eRF3c; ERFS; HBS1; HSPC276"
                     /note="hexamer: AAGAAA"
     polyA_site      6402
                     /gene="HBS1L"
                     /gene_synonym="EF-1a; eRF3c; ERFS; HBS1; HSPC276"
ORIGIN      
        1 ggcgcagagg cctgcgggaa gccaagatgg cgcatagggg ttctccaggc tgcagttggc
       61 gccttatcag tatctaagcg gagtgttttg gaaggagtta aggggctgtg gcaaacgccc
      121 tctccgccgt catggcccgg catcggaatg ttcgaggcta taactacgat gaagattttg
      181 aagatgatga tctctacggc cagtctgtag aggatgatta ttgtatttcg ccgtcaacag
      241 ctgctcagtt tatttattca cggcgtgaca aaccttccgt tgagcctgtg gaagaatatg
      301 attatgaaga tctgaaagaa tcttccaatt ctgtttcaaa ccatcagctc agtggatttg
      361 atcaagctcg tctttattca tgccttgatc acatgagaga ggtacttgga gatgctgtgc
      421 cagatgaaat attaattgaa gcagttctga agaacaagtt tgatgtgcag aaggctttgt
      481 caggggttct ggaacaagat agagtgcaga gtttgaagga caagaatgag gcaacagtat
      541 ctacaggaaa gatagcaaaa ggaaaaccag tagattccca gacatcgcga agtgaatctg
      601 aaattgtgcc aaaagttgct aaaatgactg tatctggaaa gaagcaaact atgggatttg
      661 aagtgcctgg agtatcttct gaagaaaatg gtcatagttt ccacacacct caaaaaggac
      721 cgcccattga agatgccatt gcttcttccg atgttcttga gactgcttct aaatctgcta
      781 atccacccca cacgattcaa gcatcagaag agcagagttc aaccccagca ccggtgaaaa
      841 agtctggcaa gctgaggcag caaatagatg tgaaggcgga actggagaag cggcaaggag
      901 ggaagcagct actcaactta gtggtcattg gtcatgttga tgctgggaaa agtactctga
      961 tgggccatat gctttatctt ctgggtaata taaacaaaag aactatgcat aagtatgaac
     1021 aggagtctaa aaaggctggc aaagcttcgt ttgcatatgc atgggtcttg gatgaaactg
     1081 gcgaagaaag ggaaagggga gtaaccatgg atgttggtat gacaaagttt gaaaccacaa
     1141 ccaaagttat tacattaatg gatgctccag gccataagga cttcattcca aatatgatta
     1201 caggagcagc ccaggcggat gtagctgttt tagttgtaga tgccagcagg ggagagtttg
     1261 aagctggatt tgagactgga ggacaaacac gagagcatgg actcttggtc cgttctctgg
     1321 gagtgacgca gcttgcagtt gcagttaata aaatggatca ggttaattgg caacaagaaa
     1381 ggtttcaaga gattactgga aaacttgggc actttcttaa gcaagcaggt tttaaggaga
     1441 gtgatgtagg ttttattcct acaagtggtc tcagtggtga aaatctaatc acaagatctc
     1501 agtcaagtga actcacaaaa tggtataaag gactatgttt attagaacaa attgattcct
     1561 ttaagcctcc ccagcgatct attgacaaac cttttagatt atgtgtgtcc gatgttttca
     1621 aagatcaagg atctggattt tgcataactg gtaaaataga agctggttat atccaaactg
     1681 gtgaccgact actggcaatg cctcctaatg aaacttgtac cgtgaaagga atcactctgc
     1741 atgatgaacc tgtcgactgg gcggcagcag gcgatcatgt tagtcttact ttggttggga
     1801 tggatatcat caaaatcaat gttggctgca tattttgtgg ccccaaagta cccattaaag
     1861 cttgcactcg tttcagagcc cgaatcctca tctttaatat tgaaattcct atcactaaag
     1921 gatttcctgt gctgttacac taccaaactg tcagtgaacc cgccgttatt aaacgattga
     1981 ttagtgtctt aaacaaaagc acgggtgaag tcacaaagaa aaagcctaag tttttgacta
     2041 aaggccagaa tgcattggta gagctacaga cacaaagacc aatagctctt gagctatata
     2101 aagactttaa agagctgggg aggttcatgc tacgttacgg tggttctaca atagctgctg
     2161 gtgttgtcac tgagataaaa gaatgatggg tcagaatttc taccacgttt ctggatacag
     2221 tgaaatagct aacctctgtt tcaagaatgc agttattaag tcaaaggaac aatgtgcaat
     2281 tgatatgttt ttagatgaga gagaaaaatt aaagctaaaa ttagctgcaa agaagtatta
     2341 ataatcacct ctgcaaaaat tctaagttgc caactggcaa agaaagtcta atgttaaaaa
     2401 caactttgcc tttgaaacgt taataaatgg atttactttg ctaagattta tggcaagtgt
     2461 caaaaatagt atctgaagat actgaatcat catgaaatga actctacttc tggccaaagc
     2521 acaatgtatt tgcagttttc tcttttgatt caattatact gcacatgttt taaggaaaag
     2581 taacttaatt gggtttttca ggcagttgat atttgaccta agcttttttt tttttttttt
     2641 ttccagttaa tgctaagaaa agatttgggg aaggttataa taaaagtatt ttgtggtgac
     2701 cataagaatg tccctcccca aacaagtaaa cttgtgaaag tttaatttgg aattagtgga
     2761 agctgttcct ttgaaagcca agatattatt taagttgtaa agccagctaa taaaatgcct
     2821 tagtttgagc ataatacaaa ctgtgttttg ttctttagac atttatgaga ttctctgcca
     2881 ctaacagtaa atatgattta ggggttttgt gggatctttt tcacctaaga gtttcctagc
     2941 tctgtcacat gaattctttt ttatcctaat ggcagatcca atttcatctc attcattttt
     3001 ttaaccataa cttgcttttc ccattctcac ttttagaatt atacctatga tggccctttc
     3061 tttatcttgc ttaccttata gattataagt cccctgtgag taagtaagag tcatgttttt
     3121 ctttaaagtt tcaacaacaa accccaaggt ttatttattt aacaacaggt attcgttgag
     3181 gtgccttctg ggtgcaagga gctttagagt agattttgac ctcagctgaa cctggaactc
     3241 tgatctgata gactgtttat gataagcttt tttttttatt agcagcgtga tttgtatatc
     3301 gatggcttaa catatacaac agcatttctt ctccctgcac cttttcgtga acctgacatt
     3361 ttgaacataa gcaataacat ctgtatggta aagtaggcag attatacatg tgttgcttag
     3421 tgaatgatta ccaagtgaac acacttggta tattgcttta ccaaaataca tgtgtagttg
     3481 tcacttcaga ttcactgagt taagaaagtt ttatttaaat attaatatta atcaagaatt
     3541 taacagatat atgtgacaga aatctaactt caagtgactg aagcagaaag aaaaattttt
     3601 gaatgacata actgagaaaa ttagaggtag ctctagcttt gattccattt agatcgagca
     3661 gctcacacaa tgttagcaag gactcatctc cactctgcct tccctggtgc tggttttgct
     3721 ctcaaactct atatagaact ctcggccggg cgcggtggct cacacctgta atcaaaacac
     3781 tttgggaggc tgagtcggga ggatagcgtg agcccaggag tttgagacca gctaaggcaa
     3841 tatagtgaga ccatgtctct acagaaaaaa tttaaaaaca gatcagctgg gtgtggtgcc
     3901 acatgcctgt agtcccagct tctcaggagg ctggggtggg aagatcactt gagcccagga
     3961 ggtcgaggct gcagtgagcc atgatcacat cactgcagtc cagcctgggt gacagagcaa
     4021 gacctgtcac cctgccaccc ataaaaaacc tcttctgtaa tgccaggttc ttgacaagat
     4081 gtgataattc cacacttaca tcctcagatc tcccatccag ggcaaggaca gactaaaaga
     4141 gcatatcttc tccagaagtc ctagaaacat ctcatcattg actatgattg aattttattt
     4201 attaatactt ccctaggata tcctgtgttt gagtgtgaag ttaatatcac acaaagcatg
     4261 tagctaacag ttgtgtgagg gttggaaatt cagggtgctg ttaggaaggg gaaatggatt
     4321 ggggtttaag gtagggggag taaaaacaag aaaccatcca ctctgtatat tatataagac
     4381 tgtgatcgaa ttaagaattt tagtttacag tttagcataa acaaatagta gtagaaatga
     4441 atgcatttat tcattccttc cttctccaat agtgtttaag tacaatcttt ttatttgcat
     4501 tttattgatg attgattgtg gttgaaagga gtggtgatat tatttcgagg atccactgtg
     4561 taagaagact gttggcagtg gaaggctcga gttgattaag tgacaaagag gtaaagcaga
     4621 catgcccaag cttggttatg ctctgatttc ctaccctgtt actttcatcc ataatttata
     4681 gtacatccat tttgtgtcat tttgcttcag gtatttcttg tcctgactgt cttctttttt
     4741 atgattttac tgtttcttca cactaatggc tttgtcctct gactttgact ctttgtgaga
     4801 ttcttctgag cagttaacct ctaggataat gtggcttgaa cacattacag aactatgttt
     4861 tgacttgtta gtattattca cacattcatg acaattgagc ctctctactg tgagaaatgt
     4921 tttagaggcc agtttctagg catagctgtt tttctcccat gagtatgcaa atcaatagtt
     4981 ttaaccattg ttaaaatgca atcatagtga cttcagaaaa tttggaaaat acagaaaata
     5041 tagggaagaa taaaattaac ccacagttct gtcacctaaa ggtaactgct gccaatgttt
     5101 tcatgtattt cttttctgtc tctccaatgc atttttttac atagttgtga gcgttagcca
     5161 agttattttc atgttgttat gtaatcaata tttttcaata gaagtattag aggttttttt
     5221 tattgatata aaaataacaa ttacagatcc tgatatatag aagttattca aaattataca
     5281 gttttcaaaa aatcaagaca agtaggccca atacaaacta ctgaatcatc ttctaatttc
     5341 cctctaaaat atttatagaa atatgtaagt agaaaaacat tcatcctttc ctcgtctaat
     5401 tatgatcctg ccatattcca ggcacaagag aaagctctgg ggcttgagtc ttaatagggc
     5461 tgatagtcca accaggggac agggtatcat aaagagataa ttcaaaactt taagattgga
     5521 gggtaggtga tggtagaaaa ttctgcggca aacatttgtt gatgctcatc atttgttgat
     5581 gtcatcaaag atcaccaggg cataattata atcaaaatta gttttattga tgcttgctgc
     5641 agcaagagag actgcacacc actggggtct atgggtgctt ctcagtggga aggtgtaagg
     5701 aggggcttgc taagaatttg agcacatgta gctaatttta aggagggctc aagtgagcaa
     5761 gggtttcttc tggattgagt gctgtcagaa agtggattga gtgctgtcag aaagtgggag
     5821 tgattttgca actgggtatc ttaattttta tgttgtgggt gggaggaaag gaaagaagca
     5881 acagtcaccc atgttagcca caagaagagg ttggctgttt atggtttgca cagtgacttt
     5941 gtttttatct gtgctcaggc atgattacag tatctcattt tatccggatt acagtgacct
     6001 catctgatgt tagagatttg agaattgtta aatgttcagc aggagaacgc caccgcctag
     6061 tgtgaatgct aggtcagaag ctgctttttc acactgaaac catttaagta tgtacaaata
     6121 agttaatagt gtaaatgtag gtgtaaaata caatgcaaaa caaaataatg aagtaagtga
     6181 attaaattat tgcaccaaat atggggatat gaaataggga aagaaaagca ttaagttact
     6241 tatagtccat aaagggacta cattattttg ttactctgat aatcccataa aacaaagttt
     6301 taagaatgtt tacagtaacc atgaatggaa ttagtgaaac tattcatatc aatgcaaaaa
     6361 aaaaaaaaaa aaaagcaaag aaactaagtc tgtacagcaa aagatggaaa ggaaacaaga
     6421 aaattaaatg aagacaaagg ttgtgattat tacagtaaat gaaaataggt ttttatcttt
     6481 aaaatgtaga ctcaaactgc aaagctaatg atatgctatc tgcaaaatat tcctattttg
     6541 taaataaata aaaccataga tttaaagaag aaagggcaca gatgtactgg ggagacaaaa
     6601 ataactgatc cgttgggtgt ataccatgtg ccaagcaatt ttcatatact catttaacat
     6661 gacatatcta taattatatg aggcagatat tattaacctc agtctataga tgagaagact
     6721 aaagctcaga gggattgcta aaaactagta aataaattat ggaaccatga tttgaattta
     6781 gatttaagtc tgactgaggc atgttcagag acactacact gcctctccag aaatcaggag
     6841 tagcaatatt aacactggac aaagaattta aagcaagtaa aaaaaaatta agagaatttc
     6901 atattaataa caagtataat gcacaataaa actgtaccaa atgacataaa aatgcataat
     6961 gaaatttatt agaaattaaa aattaaaggg catagttaca attattgagg aattctcata
     7021 gctctgcctt atagatcatg taaacaaaca aggtcataga ggacttaaat agcaaagcag
     7081 aaaaaaa
//
