LOCUS       NM_033417               1407 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens HAUS augmin like complex subunit 8 (HAUS8), transcript
            variant 1, mRNA.
ACCESSION   NM_033417 XM_050564
VERSION     NM_033417.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   2  (bases 1 to 1407)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 1407)
  AUTHORS   Luo,J., Yang,B., Xin,G., Sun,M., Zhang,B., Guo,X., Jiang,Q. and
            Zhang,C.
  TITLE     The microtubule-associated protein EML3 regulates mitotic spindle
            assembly by recruiting the Augmin complex to spindle microtubules
  JOURNAL   J Biol Chem 294 (14), 5643-5656 (2019)
   PUBMED   30723163
REFERENCE   4  (bases 1 to 1407)
  AUTHORS   He,T.S., Chen,T., Wang,D.D. and Xu,L.G.
  TITLE     HAUS8 regulates RLR-VISA antiviral signaling positively by
            targeting VISA
  JOURNAL   Mol Med Rep 18 (2), 2458-2466 (2018)
   PUBMED   29916539
  REMARK    GeneRIF: these results demonstrated that HAUS8 may function as a
            positive regulator of RLRVISA dependent antiviral signaling by
            targeting the VISA complex, providing a novel regulatory mechanism
            of antiviral responses
REFERENCE   5  (bases 1 to 1407)
  AUTHORS   Cunha-Ferreira,I., Chazeau,A., Buijs,R.R., Stucchi,R., Will,L.,
            Pan,X., Adolfs,Y., van der Meer,C., Wolthuis,J.C., Kahn,O.I.,
            Schatzle,P., Altelaar,M., Pasterkamp,R.J., Kapitein,L.C. and
            Hoogenraad,C.C.
  TITLE     The HAUS Complex Is a Key Regulator of Non-centrosomal Microtubule
            Organization during Neuronal Development
  JOURNAL   Cell Rep 24 (4), 791-800 (2018)
   PUBMED   30044976
REFERENCE   6  (bases 1 to 1407)
  AUTHORS   Lawo,S., Bashkurov,M., Mullin,M., Ferreria,M.G., Kittler,R.,
            Habermann,B., Tagliaferro,A., Poser,I., Hutchins,J.R., Hegemann,B.,
            Pinchev,D., Buchholz,F., Peters,J.M., Hyman,A.A., Gingras,A.C. and
            Pelletier,L.
  TITLE     HAUS, the 8-subunit human Augmin complex, regulates centrosome and
            spindle integrity
  JOURNAL   Curr Biol 19 (10), 816-826 (2009)
   PUBMED   19427217
REFERENCE   7  (bases 1 to 1407)
  AUTHORS   Uehara,R., Nozawa,R.S., Tomioka,A., Petry,S., Vale,R.D., Obuse,C.
            and Goshima,G.
  TITLE     The augmin complex plays a critical role in spindle microtubule
            generation for mitotic progression and cytokinesis in human cells
  JOURNAL   Proc Natl Acad Sci U S A 106 (17), 6998-7003 (2009)
   PUBMED   19369198
REFERENCE   8  (bases 1 to 1407)
  AUTHORS   Wu,G., Lin,Y.T., Wei,R., Chen,Y., Shan,Z. and Lee,W.H.
  TITLE     Hice1, a novel microtubule-associated protein required for
            maintenance of spindle integrity and chromosomal stability in human
            cells
  JOURNAL   Mol Cell Biol 28 (11), 3652-3662 (2008)
   PUBMED   18362163
  REMARK    GeneRIF: Hice1 is a novel microtubule-associated protein important
            for maintaining spindle integrity and chromosomal stability, in
            part by virtue of its ability to bind, bundle, and stabilize
            microtubules.
REFERENCE   9  (bases 1 to 1407)
  AUTHORS   Goshima,G., Mayer,M., Zhang,N., Stuurman,N. and Vale,R.D.
  TITLE     Augmin: a protein complex required for centrosome-independent
            microtubule generation within the spindle
  JOURNAL   J Cell Biol 181 (3), 421-429 (2008)
   PUBMED   18443220
REFERENCE   10 (bases 1 to 1407)
  AUTHORS   Lee,S.Y., Obata,Y., Yoshida,M., Stockert,E., Williamson,B.,
            Jungbluth,A.A., Chen,Y.T., Old,L.J. and Scanlan,M.J.
  TITLE     Immunomic analysis of human sarcoma
  JOURNAL   Proc Natl Acad Sci U S A 100 (5), 2651-2656 (2003)
   PUBMED   12601173
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BI829542.1, BC040564.1, BX111116.1 and AC020908.7.
            
            On Nov 22, 2018 this sequence version replaced NM_033417.1.
            
            Summary: HAUS8 is 1 of 8 subunits of the 390-kD human augmin
            complex, or HAUS complex. The augmin complex was first identified
            in Drosophila, and its name comes from the Latin verb 'augmentare,'
            meaning 'to increase.' The augmin complex is a microtubule-binding
            complex involved in microtubule generation within the mitotic
            spindle and is vital to mitotic spindle assembly (Goshima et al.,
            2008 [PubMed 18443220]; Uehara et al., 2009 [PubMed
            19369198]).[supplied by OMIM, Jun 2010].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC004398.1, SRR5189658.110625.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000253669.10/ ENSP00000253669.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-243               BI829542.1         82-324
            244-1368            BC040564.1         2050-3174
            1369-1375           BX111116.1         13-19               c
            1376-1407           AC020908.7         120749-120780       c
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19p13.11"
     gene            1..1407
                     /gene="HAUS8"
                     /gene_synonym="DGT4; HICE1; NY-SAR-48"
                     /note="HAUS augmin like complex subunit 8"
                     /db_xref="GeneID:93323"
                     /db_xref="HGNC:HGNC:30532"
                     /db_xref="MIM:613434"
     exon            1..59
                     /gene="HAUS8"
                     /gene_synonym="DGT4; HICE1; NY-SAR-48"
                     /inference="alignment:Splign:2.1.0"
     CDS             31..1263
                     /gene="HAUS8"
                     /gene_synonym="DGT4; HICE1; NY-SAR-48"
                     /note="isoform a is encoded by transcript variant 1;
                     Hec1-interacting and centrosome-associated 1; HEC1/NDC80
                     interacting, centrosome associated 1; sarcoma antigen
                     NY-SAR-48; HEC1/NDC80-interacting centrosome-associated
                     protein 1"
                     /codon_start=1
                     /product="HAUS augmin-like complex subunit 8 isoform a"
                     /protein_id="NP_219485.1"
                     /db_xref="CCDS:CCDS32948.1"
                     /db_xref="GeneID:93323"
                     /db_xref="HGNC:HGNC:30532"
                     /db_xref="MIM:613434"
                     /translation="MADSSGRGAGKPATGPTNSSSAKKKDKRVQGGRVIESRYLQYEK
                     KTTQKAPAGDGSQTRGKMSEGGRKSSLLQKSKADSSGVGKGDLQSTLLEGHGTAPPDL
                     DLSAINDKSIVKKTPQLAKTISKKPESTSFSAPRKKSPDLSEAMEMMESQTLLLTLLS
                     VKMENNLAEFERRAEKNLLIMCKEKEKLQKKAHELKRRLLLSQRKRELADVLDAQIEM
                     LSPFEAVATRFKEQYRTFATALDTTRHELPVRSIHLEGDGQQLLDALQHELVTTQRLL
                     GELDVGDSEENVQVLDLLSELKDVTAKKDLELRRSFAQVLELSAEASKEAALANQEVW
                     EETQGMAPPSRWYFNQDSACRESGGAPKNTPLSEDDNPGASSAPAQATFISPSEDFSS
                     SSQAEVPPSLSRSGRDLS"
     exon            60..121
                     /gene="HAUS8"
                     /gene_synonym="DGT4; HICE1; NY-SAR-48"
                     /inference="alignment:Splign:2.1.0"
     exon            122..177
                     /gene="HAUS8"
                     /gene_synonym="DGT4; HICE1; NY-SAR-48"
                     /inference="alignment:Splign:2.1.0"
     exon            178..259
                     /gene="HAUS8"
                     /gene_synonym="DGT4; HICE1; NY-SAR-48"
                     /inference="alignment:Splign:2.1.0"
     exon            260..355
                     /gene="HAUS8"
                     /gene_synonym="DGT4; HICE1; NY-SAR-48"
                     /inference="alignment:Splign:2.1.0"
     exon            356..450
                     /gene="HAUS8"
                     /gene_synonym="DGT4; HICE1; NY-SAR-48"
                     /inference="alignment:Splign:2.1.0"
     exon            451..516
                     /gene="HAUS8"
                     /gene_synonym="DGT4; HICE1; NY-SAR-48"
                     /inference="alignment:Splign:2.1.0"
     exon            517..675
                     /gene="HAUS8"
                     /gene_synonym="DGT4; HICE1; NY-SAR-48"
                     /inference="alignment:Splign:2.1.0"
     exon            676..817
                     /gene="HAUS8"
                     /gene_synonym="DGT4; HICE1; NY-SAR-48"
                     /inference="alignment:Splign:2.1.0"
     exon            818..959
                     /gene="HAUS8"
                     /gene_synonym="DGT4; HICE1; NY-SAR-48"
                     /inference="alignment:Splign:2.1.0"
     exon            960..1407
                     /gene="HAUS8"
                     /gene_synonym="DGT4; HICE1; NY-SAR-48"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1353..1358
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HAUS8"
                     /gene_synonym="DGT4; HICE1; NY-SAR-48"
                     /note="hexamer: AGTAAA"
     polyA_site      1375
                     /gene="HAUS8"
                     /gene_synonym="DGT4; HICE1; NY-SAR-48"
                     /note="major polyA site"
     polyA_site      1407
                     /gene="HAUS8"
                     /gene_synonym="DGT4; HICE1; NY-SAR-48"
ORIGIN      
        1 gggtcgggcc ttgaggtgga aggcgggaaa atggcggatt cctcggggcg aggcgctggg
       61 aagcctgcaa ccggccccac aaattctagc agtgccaaga agaaggataa aagagttcaa
      121 ggtggaagag tgattgagtc ccggtatctg cagtatgaaa agaagacaac ccaaaaggct
      181 cctgcaggag atgggtcaca gacccgaggg aagatgtctg aaggtggaag gaaatccagc
      241 ctgctccaga aaagcaaagc agatagcagt ggggtcggaa agggtgacct gcagtccacg
      301 ttgctggaag ggcatggcac agctccacct gacctggatc tctctgctat taatgacaaa
      361 agcatcgtca aaaagacgcc acagttagca aaaacaatat caaagaaacc tgagtcaaca
      421 tcattttctg cccctcggaa aaagagcccg gatttatctg aagcaatgga aatgatggag
      481 tctcagacac tactgctgac gctactatcc gtaaagatgg agaacaatct tgctgagttt
      541 gaaagaaggg cagaaaagaa tttattaata atgtgtaagg agaaggagaa gctacagaaa
      601 aaggcccacg agctgaagcg caggcttctc ctctctcaga ggaagcggga gctggcagat
      661 gtcctggatg cccagatcga gatgctcagc cccttcgagg cagtggccac acgcttcaag
      721 gagcaataca ggacattcgc cacggccctg gacactacca ggcacgagct gcccgtgagg
      781 tccatccacc tggagggaga tgggcagcag ctcttagacg ccctgcagca tgaactggtg
      841 accactcagc gcctcctggg agaacttgat gttggtgatt cggaagaaaa tgtgcaggtg
      901 ctggacttac tgagcgaact caaggacgtg acggcgaaaa aggaccttga gctccgaagg
      961 agctttgccc aggtgctgga actctccgca gaggcaagca aagaggcagc cttggcaaac
     1021 caggaagtct gggaagagac ccagggcatg gcgcccccca gccggtggta tttcaatcaa
     1081 gacagtgcct gcagagaatc tgggggagca cccaagaaca cgcccctgtc tgaggacgac
     1141 aacccgggtg cctcgtcagc ccccgctcag gccacgttca tcagcccaag cgaagatttt
     1201 tcttcaagca gccaggcaga agtcccgccc tctctctctc gttcagggag ggacttgtca
     1261 tgactcatgg ttacattcag gatacttgag cactttatat actaccgtag cactgtagct
     1321 attttttatg tgataatctt gtttgataaa aaagtaaacc tgttttgcaa ttgaagcctc
     1381 cattttctat gaaggggcca gttggaa
//
