LOCUS       NM_018097               3921 bp    mRNA    linear   PRI 05-APR-2024
DEFINITION  Homo sapiens HAUS augmin like complex subunit 2 (HAUS2), transcript
            variant 1, mRNA.
ACCESSION   NM_018097
VERSION     NM_018097.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 3921)
  AUTHORS   Luo,J., Yang,B., Xin,G., Sun,M., Zhang,B., Guo,X., Jiang,Q. and
            Zhang,C.
  TITLE     The microtubule-associated protein EML3 regulates mitotic spindle
            assembly by recruiting the Augmin complex to spindle microtubules
  JOURNAL   J Biol Chem 294 (14), 5643-5656 (2019)
   PUBMED   30723163
REFERENCE   3  (bases 1 to 3921)
  AUTHORS   Cunha-Ferreira,I., Chazeau,A., Buijs,R.R., Stucchi,R., Will,L.,
            Pan,X., Adolfs,Y., van der Meer,C., Wolthuis,J.C., Kahn,O.I.,
            Schatzle,P., Altelaar,M., Pasterkamp,R.J., Kapitein,L.C. and
            Hoogenraad,C.C.
  TITLE     The HAUS Complex Is a Key Regulator of Non-centrosomal Microtubule
            Organization during Neuronal Development
  JOURNAL   Cell Rep 24 (4), 791-800 (2018)
   PUBMED   30044976
REFERENCE   4  (bases 1 to 3921)
  AUTHORS   Hein,M.Y., Hubner,N.C., Poser,I., Cox,J., Nagaraj,N., Toyoda,Y.,
            Gak,I.A., Weisswange,I., Mansfeld,J., Buchholz,F., Hyman,A.A. and
            Mann,M.
  TITLE     A human interactome in three quantitative dimensions organized by
            stoichiometries and abundances
  JOURNAL   Cell 163 (3), 712-723 (2015)
   PUBMED   26496610
REFERENCE   5  (bases 1 to 3921)
  AUTHORS   Hsia,K.C., Wilson-Kubalek,E.M., Dottore,A., Hao,Q., Tsai,K.L.,
            Forth,S., Shimamoto,Y., Milligan,R.A. and Kapoor,T.M.
  TITLE     Reconstitution of the augmin complex provides insights into its
            architecture and function
  JOURNAL   Nat Cell Biol 16 (9), 852-863 (2014)
   PUBMED   25173975
REFERENCE   6  (bases 1 to 3921)
  AUTHORS   Casenghi,M., Meraldi,P., Weinhart,U., Duncan,P.I., Korner,R. and
            Nigg,E.A.
  TITLE     Polo-like kinase 1 regulates Nlp, a centrosome protein involved in
            microtubule nucleation
  JOURNAL   Dev Cell 5 (1), 113-125 (2003)
   PUBMED   12852856
REFERENCE   7  (bases 1 to 3921)
  AUTHORS   Dgany,O., Avidan,N., Delaunay,J., Krasnov,T., Shalmon,L.,
            Shalev,H., Eidelitz-Markus,T., Kapelushnik,J., Cattan,D.,
            Pariente,A., Tulliez,M., Cretien,A., Schischmanoff,P.O.,
            Iolascon,A., Fibach,E., Koren,A., Rossler,J., Le Merrer,M.,
            Yaniv,I., Zaizov,R., Ben-Asher,E., Olender,T., Lancet,D.,
            Beckmann,J.S. and Tamary,H.
  TITLE     Congenital dyserythropoietic anemia type I is caused by mutations
            in codanin-1
  JOURNAL   Am J Hum Genet 71 (6), 1467-1474 (2002)
   PUBMED   12434312
REFERENCE   8  (bases 1 to 3921)
  AUTHORS   Takahashi,M., Yamagiwa,A., Nishimura,T., Mukai,H. and Ono,Y.
  TITLE     Centrosomal proteins CG-NAP and kendrin provide microtubule
            nucleation sites by anchoring gamma-tubulin ring complex
  JOURNAL   Mol Biol Cell 13 (9), 3235-3245 (2002)
   PUBMED   12221128
REFERENCE   9  (bases 1 to 3921)
  AUTHORS   Mayor,T., Stierhof,Y.D., Tanaka,K., Fry,A.M. and Nigg,E.A.
  TITLE     The centrosomal protein C-Nap1 is required for cell cycle-regulated
            centrosome cohesion
  JOURNAL   J Cell Biol 151 (4), 837-846 (2000)
   PUBMED   11076968
REFERENCE   10 (bases 1 to 3921)
  AUTHORS   Golsteyn,R.M., Mundt,K.E., Fry,A.M. and Nigg,E.A.
  TITLE     Cell cycle regulation of the activity and subcellular localization
            of Plk1, a human protein kinase implicated in mitotic spindle
            function
  JOURNAL   J Cell Biol 129 (6), 1617-1628 (1995)
   PUBMED   7790358
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC010903.1, AC018362.8 and AI368236.1.
            
            On Nov 23, 2018 this sequence version replaced NM_018097.2.
            
            Summary: The protein encoded by this gene is a subunit of the
            augmin complex. The augmin complex plays a role in microtubule
            attachment to the kinetochore and central spindle formation.
            [provided by RefSeq, Apr 2016].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and it encodes the longer protein (isoform 1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            transcript and genomic sequence to make the sequence consistent
            with the reference genome assembly. The genomic coordinates used
            for the transcript record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BP225980.1, SRR1803617.75930.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000260372.8/ ENSP00000260372.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-788               BC010903.1         5-792
            789-3565            AC018362.8         103963-106739
            3566-3921           AI368236.1         8-363               c
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q15.2"
     gene            1..3921
                     /gene="HAUS2"
                     /gene_synonym="C15orf25; CEP27; HsT17025"
                     /note="HAUS augmin like complex subunit 2"
                     /db_xref="GeneID:55142"
                     /db_xref="HGNC:HGNC:25530"
                     /db_xref="MIM:613429"
     exon            1..128
                     /gene="HAUS2"
                     /gene_synonym="C15orf25; CEP27; HsT17025"
                     /inference="alignment:Splign:2.1.0"
     CDS             36..743
                     /gene="HAUS2"
                     /gene_synonym="C15orf25; CEP27; HsT17025"
                     /note="isoform 1 is encoded by transcript variant 1;
                     centrosomal protein 27kDa; centrosomal protein of 27 kDa"
                     /codon_start=1
                     /product="HAUS augmin-like complex subunit 2 isoform 1"
                     /protein_id="NP_060567.1"
                     /db_xref="CCDS:CCDS10090.1"
                     /db_xref="GeneID:55142"
                     /db_xref="HGNC:HGNC:25530"
                     /db_xref="MIM:613429"
                     /translation="MAAANPWDPASAPNGAGLVLGHFIASGMVNQEMLNMSKKTVSCF
                     VNFTRLQQITNIQAEIYQKNLEIELLKLEKDTADVVHPFFLAQKCHTLQSMNNHLEAV
                     LKEKRSLRQRLLKPMCQENLPIEAVYHRYMVHLLELAVTFIERLETHLETIRNIPHLA
                     ANLKKMNQALAKMDILVTETEELAENILKWRKQQNEVSSCIPKILAEESYLYKHDIIM
                     PPLPFTSKVHVQTINAK"
     exon            129..221
                     /gene="HAUS2"
                     /gene_synonym="C15orf25; CEP27; HsT17025"
                     /inference="alignment:Splign:2.1.0"
     exon            222..291
                     /gene="HAUS2"
                     /gene_synonym="C15orf25; CEP27; HsT17025"
                     /inference="alignment:Splign:2.1.0"
     exon            292..424
                     /gene="HAUS2"
                     /gene_synonym="C15orf25; CEP27; HsT17025"
                     /inference="alignment:Splign:2.1.0"
     exon            425..533
                     /gene="HAUS2"
                     /gene_synonym="C15orf25; CEP27; HsT17025"
                     /inference="alignment:Splign:2.1.0"
     exon            534..3921
                     /gene="HAUS2"
                     /gene_synonym="C15orf25; CEP27; HsT17025"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2834..2839
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HAUS2"
                     /gene_synonym="C15orf25; CEP27; HsT17025"
                     /note="hexamer: AATAAA"
     polyA_site      2856
                     /gene="HAUS2"
                     /gene_synonym="C15orf25; CEP27; HsT17025"
     regulatory      3896..3901
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HAUS2"
                     /gene_synonym="C15orf25; CEP27; HsT17025"
                     /note="hexamer: AATAAA"
     polyA_site      3921
                     /gene="HAUS2"
                     /gene_synonym="C15orf25; CEP27; HsT17025"
                     /note="major polyA site"
ORIGIN      
        1 actcttggcg ccttcgcgga aggtgcgtcc gagccatggc cgctgccaac ccgtgggacc
       61 cggcgtccgc gcctaacggc gctgggctag tgctaggcca cttcatagct tcggggatgg
      121 tcaatcagga gatgttaaac atgtctaaga aaacagtttc ttgttttgtg aacttcacca
      181 gactacagca gatcacaaat attcaagctg aaatctacca gaaaaacctg gaaattgaac
      241 tcctgaaact agaaaaagat acagcagatg ttgttcatcc tttctttttg gctcagaagt
      301 gtcatactct gcaaagcatg aataatcatt tggaagcagt gctgaaagag aagagatccc
      361 ttaggcaaag actgttgaaa cccatgtgcc aggaaaactt acctattgaa gctgtttatc
      421 acagatatat ggtacatttg ctggagttgg ctgtgacttt cattgagaga ttagaaaccc
      481 accttgaaac aattagaaat attcctcatt tagctgcaaa tctaaagaaa atgaaccagg
      541 ctttagcaaa gatggatata ttggtgactg agacagaaga actggcagag aatatactca
      601 agtggcgtaa acaacaaaac gaagtttcgt cttgtatccc caaaatatta gctgaagaaa
      661 gttatcttta taaacatgat attataatgc ctcctttacc ttttacttct aaagttcatg
      721 tccaaactat taatgccaag tagtcatcaa ctttattttt gcttaattat gtgtagtcat
      781 atgaagtcta tttctagttg actgtaacat gggtattaat agtctttgct gctggtaata
      841 ctgaaagaac ctgctttata ttggagtatc aagatctcag gttcattaag accaaactga
      901 cttttccttt gtttttcata tatttttatt ctacctttca gtaaaactag agaagctaaa
      961 aaatagccat gacaatttat taatataagt gaattaacca tttctcaggt gggaaattct
     1021 ctttttttaa aaatgtgtgt ttatcgtctg gtgtggtggc tcacacctgt aatcctaaca
     1081 cttggggagg ctgaggcggg cagatcactt aaacccagga gttcaagacc agcctgggta
     1141 acatggtgaa atcccatatc tacaaaataa acaaaaaatt agccgaccat ggtggtgcat
     1201 gcctgtagtc ccagctattc gggaggctga ggtaagagga tcacctaagc ctgtgaggtc
     1261 atggttgcaa tgagtcatga tcacgccact gcgctacagc ctgggcgaca cagtaagacc
     1321 ctgtctcaaa aaaaagaagt gtgtttctgg ccaggcacgg tggctcacgc ctgtaatccc
     1381 agcactttgg gaggcctagg tgggcagatc atgaggtcag gagttcgaga ccagcctggc
     1441 caacatggcg aaacacctgt ctctactaaa aatacaaaaa ttaggccggg cgcagtggct
     1501 cacacctgta atcccaacac tttgggaggc cgacccagat gggtggatca cctgaggtca
     1561 ggagtttgag accagccttg acaacatggt gaaaccccat ctctggtaga aatataaaaa
     1621 attaaccggg catggtggtg gacgcctgta atcccagcta cttgggaggc tgaggcagaa
     1681 taatcgcttg aacccaggag gcagaggttg cagtaagcca agatcgtgcc tctgcactcc
     1741 aacctgggtg atggagcaag actccatctc caaaaaaata aaaaatacaa aaattagcca
     1801 ggcatggtag catgtgcctg taatcccagc tactcaggag gctgaggcag gagaatcact
     1861 tgaacccagg aggcagaggt tgcagtgagc ccagatcgtg ccaccgcact ctagcctggg
     1921 caacagagca agactctgtc ttaaaaaaaa aaaaaaaagg tgtttctgaa ttagaagggt
     1981 cttcttgaat ctgaaaatgt ctatgatgat tattcctgtg ggaatgtcac taagtatgca
     2041 gaaaagagta acacagccag cctgacttat cctttgaaag gcctgattat aaggtttgtc
     2101 ctcagctgtt gcatctggga acttagatat cagaaggtat cttagtctgt tctgctgtca
     2161 taacagaatt acacaaacta agtattttat aatgaacaga agtttatttg gttgacaggt
     2221 gtggaggcta ggcagtccaa gaacatggtg ccagcatcta gtgagggtct tgccatggca
     2281 gaaggcacca catggcaagt gagcatgaga aagatatatg ggtctaaact tagcctttta
     2341 tcatgagccc actcccaaga tagcagcact aattcattaa tgagggcaaa gccctcctta
     2401 cctaattact ttttaaaggt ctcataacac ttttacaatg tcaattaaat ttccaacact
     2461 tgaactttgg gggacatatt taaaccttaa caggagggct ccactcactg aaaagagtcg
     2521 ttcatggtag ctaacctgtg gtttgtgctg aaaacctgct ttcttcctgg gagtctagaa
     2581 ttttggtaca tggtaggtag aaggtaccta cgtgaccagc ccccagtaaa aaccctgggc
     2641 actgtatctc taacaagttt ccctggtaaa caacatttct cacatgttgt cacaattcag
     2701 ttgctggtgg aatgaaacaa cagatatgtg tgactccact gttagaggac tctggaagct
     2761 tatacctggt ttcccccaga ctttgcctct gtaccttttc tctttgctgt tttgcttcat
     2821 atgcttttgc tgtaataaat catagctgtg tgatgatatg ctgagtcttg tgaattctcc
     2881 tagcaaatca tggaacctgg aggtagtctt ggagaccctg gcatacaaag cagttcctaa
     2941 gtgtttactg gtagctgtag gaataggcaa agaagaaaca gcaaagaaaa aggcacactg
     3001 tggtataact ttggaggttt accttgcaaa atgctcagat attttaccta tcagaggtta
     3061 ttgtgcatat aatagagagt gatatactaa caactgcctc aggatatact ctttttaatc
     3121 agtattgtaa ctaaccttgg cttattttac ttttagactt ggggttctat tttgctttaa
     3181 aacatgtaca tcagttttgt tttttgtttt gttcttttct ttcctttttt tttttttttt
     3241 tttaaagaca ggatctcact ctgtcaccca ggttgctgag agtgtggtgt ggcccaatct
     3301 tgacctcact gcagccttga cctctcaggc tcaagtgatc ctcccacctc agcctcccaa
     3361 gtagctggga ctataggcac gcgccaccac gcctggctaa tttttgtatt ttctgtagag
     3421 acagagtttt gccatgttgt tgaggctggt cttgaactcc tgggttcaaa cgaccctccc
     3481 gcctcagcct ccgaaagtgc tgggagtaca ggtgttagcc actgcgcctg gcctcattgt
     3541 actccttaac acaagaagac ttcaacaatg ataagtagtt gtttataagg aagcaggatc
     3601 attaccaaaa taaatcctgc taaaacaaca ggaatcatgt tttaaagcct agtttgctaa
     3661 tttttgctag taggataaga gtgatcgtaa tatctcgaac attacataga cacttaaaac
     3721 ctttagttgt atttcatcaa aaatctgttc ataccccacg ttggtttcaa aacatactat
     3781 gctttttctt cgtgttattt cctatattca tttttgtgtg tatgtgtatg tcacaaatat
     3841 tgatatgcct ggttgtttat ttttgttttc tattatgcct ttttcaaaat ataaaaataa
     3901 acttgtaatt tctaactaaa a
//
