LOCUS       NM_020771               4575 bp    mRNA    linear   PRI 06-DEC-2024
DEFINITION  Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin
            protein ligase 1 (HACE1), transcript variant 1, mRNA.
ACCESSION   NM_020771
VERSION     NM_020771.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Usmani,M.A., Ghaffar,A., Shahzad,M., Akram,J., Majeed,A.I.,
            Malik,K., Fatima,K., Khan,A.A., Ahmed,Z.M., Riazuddin,S. and
            Riazuddin,S.
  TITLE     A Missense Variant in HACE1 Is Associated with Intellectual
            Disability, Epilepsy, Spasticity, and Psychomotor Impairment in a
            Pakistani Kindred
  JOURNAL   Genes (Basel) 15 (5), 580 (2024)
   PUBMED   38790209
  REMARK    GeneRIF: A Missense Variant in HACE1 Is Associated with
            Intellectual Disability, Epilepsy, Spasticity, and Psychomotor
            Impairment in a Pakistani Kindred.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4575)
  AUTHORS   Zang,C., Liu,H., Ning,J., Chen,Q., Jiang,Y., Shang,M., Yang,Y.,
            Ma,J., Dong,Y., Wang,J., Li,F., Bao,X. and Zhang,D.
  TITLE     Emerging role and mechanism of HACE1 in the pathogenesis of
            neurodegenerative diseases: A promising target
  JOURNAL   Biomed Pharmacother 172, 116204 (2024)
   PUBMED   38364733
  REMARK    GeneRIF: Emerging role and mechanism of HACE1 in the pathogenesis
            of neurodegenerative diseases: A promising target.
            Review article
REFERENCE   3  (bases 1 to 4575)
  AUTHORS   Yin,P., Wu,Y., Long,X., Zhu,S., Chen,S., Lu,F., Lin,K. and Xu,J.
  TITLE     HACE1 expression in heart failure patients might promote
            mitochondrial oxidative stress and ferroptosis by targeting NRF2
  JOURNAL   Aging (Albany NY) 15 (23), 13888-13900 (2023)
   PUBMED   38070140
  REMARK    GeneRIF: HACE1 expression in heart failure patients might promote
            mitochondrial oxidative stress and ferroptosis by targeting NRF2.
REFERENCE   4  (bases 1 to 4575)
  AUTHORS   Singh,S., Machida,S., Tulsian,N.K., Choong,Y.K., Ng,J., Shankar,S.,
            Liu,Y., Chandiramani,K.V., Shi,J. and Sivaraman,J.
  TITLE     Structural Basis for the Enzymatic Activity of the HACE1 HECT-Type
            E3 Ligase Through N-Terminal Helix Dimerization
  JOURNAL   Adv Sci (Weinh) 10 (27), e2207672 (2023)
   PUBMED   37537642
  REMARK    GeneRIF: Structural Basis for the Enzymatic Activity of the HACE1
            HECT-Type E3 Ligase Through N-Terminal Helix Dimerization.
REFERENCE   5  (bases 1 to 4575)
  AUTHORS   Da,C., Pu,J., Liu,Z., Wei,J., Qu,Y., Wu,Y., Shi,B., Yang,J., He,N.
            and Hou,P.
  TITLE     HACE1-mediated NRF2 activation causes enhanced malignant phenotypes
            and decreased radiosensitivity of glioma cells
  JOURNAL   Signal Transduct Target Ther 6 (1), 399 (2021)
   PUBMED   34815381
  REMARK    GeneRIF: HACE1-mediated NRF2 activation causes enhanced malignant
            phenotypes and decreased radiosensitivity of glioma cells.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4575)
  AUTHORS   Sulem,P., Gudbjartsson,D.F., Rafnar,T., Holm,H., Olafsdottir,E.J.,
            Olafsdottir,G.H., Jonsson,T., Alexandersen,P., Feenstra,B.,
            Boyd,H.A., Aben,K.K., Verbeek,A.L., Roeleveld,N., Jonasdottir,A.,
            Styrkarsdottir,U., Steinthorsdottir,V., Karason,A., Stacey,S.N.,
            Gudmundsson,J., Jakobsdottir,M., Thorleifsson,G., Hardarson,G.,
            Gulcher,J., Kong,A., Kiemeney,L.A., Melbye,M., Christiansen,C.,
            Tryggvadottir,L., Thorsteinsdottir,U. and Stefansson,K.
  TITLE     Genome-wide association study identifies sequence variants on 6q21
            associated with age at menarche
  JOURNAL   Nat Genet 41 (6), 734-738 (2009)
   PUBMED   19448622
REFERENCE   7  (bases 1 to 4575)
  AUTHORS   Hibi,K., Sakata,M., Sakuraba,K., Shirahata,A., Goto,T.,
            Mizukami,H., Saito,M., Ishibashi,K., Kigawa,G., Nemoto,H. and
            Sanada,Y.
  TITLE     Aberrant methylation of the HACE1 gene is frequently detected in
            advanced colorectal cancer
  JOURNAL   Anticancer Res 28 (3A), 1581-1584 (2008)
   PUBMED   18630515
  REMARK    GeneRIF: Aberrant methylation of the HACE1 gene was detected in 9
            out of the 32 (28%) primary colon carcinomas.
REFERENCE   8  (bases 1 to 4575)
  AUTHORS   Hwang,S.J., Yang,Q., Meigs,J.B., Pearce,E.N. and Fox,C.S.
  TITLE     A genome-wide association for kidney function and endocrine-related
            traits in the NHLBI's Framingham Heart Study
  JOURNAL   BMC Med Genet 8 Suppl 1 (Suppl 1), S10 (2007)
   PUBMED   17903292
  REMARK    Publication Status: Online-Only
REFERENCE   9  (bases 1 to 4575)
  AUTHORS   Zhang,L., Anglesio,M.S., O'Sullivan,M., Zhang,F., Yang,G.,
            Sarao,R., Mai,P.N., Cronin,S., Hara,H., Melnyk,N., Li,L., Wada,T.,
            Liu,P.P., Farrar,J., Arceci,R.J., Sorensen,P.H. and Penninger,J.M.
  TITLE     The E3 ligase HACE1 is a critical chromosome 6q21 tumor suppressor
            involved in multiple cancers
  JOURNAL   Nat Med 13 (9), 1060-1069 (2007)
   PUBMED   17694067
  REMARK    GeneRIF: HACE1 is a candidate chromosome 6q21 tumor-suppressor gene
            involved in multiple cancers.
REFERENCE   10 (bases 1 to 4575)
  AUTHORS   Anglesio,M.S., Evdokimova,V., Melnyk,N., Zhang,L., Fernandez,C.V.,
            Grundy,P.E., Leach,S., Marra,M.A., Brooks-Wilson,A.R., Penninger,J.
            and Sorensen,P.H.
  TITLE     Differential expression of a novel ankyrin containing E3
            ubiquitin-protein ligase, Hace1, in sporadic Wilms' tumor versus
            normal kidney
  JOURNAL   Hum Mol Genet 13 (18), 2061-2074 (2004)
   PUBMED   15254018
  REMARK    GeneRIF: Hace1 as a novel ubiquitin-protein ligase with very low
            expression Wilms' tumors; hypermethylation of two upstream CpG
            islands correlates with low HACE1 expression in tumor samples.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK131207.1, BC034982.2,
            BC038119.1 and AJ420474.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_020771.3.
            
            Summary: This gene encodes a HECT domain and ankyrin
            repeat-containing ubiquitin ligase. The encoded protein is involved
            in specific tagging of target proteins, leading to their
            subcellular localization or proteasomal degradation. The protein is
            a potential tumor suppressor and is involved in the pathophysiology
            of several tumors, including Wilm's tumor. [provided by RefSeq, Mar
            2016].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC034982.2, SRR11853564.23766.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000262903.9/ ENSP00000262903.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-327               AK131207.1         1-327
            328-1397            BC034982.2         292-1361
            1398-1843           BC038119.1         1451-1896
            1844-3340           BC034982.2         1808-3304
            3341-4575           AJ420474.1         518-1752
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q16.3"
     gene            1..4575
                     /gene="HACE1"
                     /gene_synonym="SPPRS"
                     /note="HECT domain and ankyrin repeat containing E3
                     ubiquitin protein ligase 1"
                     /db_xref="GeneID:57531"
                     /db_xref="HGNC:HGNC:21033"
                     /db_xref="MIM:610876"
     exon            1..353
                     /gene="HACE1"
                     /gene_synonym="SPPRS"
                     /inference="alignment:Splign:2.1.0"
     CDS             278..3007
                     /gene="HACE1"
                     /gene_synonym="SPPRS"
                     /EC_number="2.3.2.26"
                     /note="isoform a is encoded by transcript variant 1;
                     HECT-type E3 ubiquitin transferase HACE1"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase HACE1 isoform a"
                     /protein_id="NP_065822.2"
                     /db_xref="CCDS:CCDS5050.1"
                     /db_xref="GeneID:57531"
                     /db_xref="HGNC:HGNC:21033"
                     /db_xref="MIM:610876"
                     /translation="MERAMEQLNRLTRSLRRARTVELPEDNETAVYTLMPMVMADQHR
                     SVSELLSNSKFDVNYAFGRVKRSLLHIAANCGSVECLVLLLKKGANPNYQDISGCTPL
                     HLAARNGQKKCMSKLLEYSADVNICNNEGLTAIHWLAVNGRTELLHDLVQHVSDVDVE
                     DAMGQTALHVACQNGHKTTVQCLLDSGADINRPNVSGATPLYFACSHGQRDTAQILLL
                     RGAKYLPDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQV
                     LEHLSQQSESQYLKILTSLAEVATTNGHKLLSLSSNYDAQMKSLLRIVRMFCHVFRIG
                     PSSPSNGIDMGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKRDSTEITSIL
                     LKQKGQDQDAASIPPFEPPGPGSYENLSTGTRESKPDALAGRQEASADCQDVISMTAN
                     RLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLEC
                     PELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSS
                     CEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTT
                     FQPNSNSYVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVAS
                     IDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEY
                     VQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIK
                     NTEYTSGYEREDPVIQWFWEVVEDITQEERVLLLQFVTGSSRVPHGGFANIMGGSGLQ
                     NFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA"
     exon            354..408
                     /gene="HACE1"
                     /gene_synonym="SPPRS"
                     /inference="alignment:Splign:2.1.0"
     exon            409..498
                     /gene="HACE1"
                     /gene_synonym="SPPRS"
                     /inference="alignment:Splign:2.1.0"
     exon            499..603
                     /gene="HACE1"
                     /gene_synonym="SPPRS"
                     /inference="alignment:Splign:2.1.0"
     exon            604..679
                     /gene="HACE1"
                     /gene_synonym="SPPRS"
                     /inference="alignment:Splign:2.1.0"
     exon            680..811
                     /gene="HACE1"
                     /gene_synonym="SPPRS"
                     /inference="alignment:Splign:2.1.0"
     exon            812..894
                     /gene="HACE1"
                     /gene_synonym="SPPRS"
                     /inference="alignment:Splign:2.1.0"
     exon            895..991
                     /gene="HACE1"
                     /gene_synonym="SPPRS"
                     /inference="alignment:Splign:2.1.0"
     exon            992..1093
                     /gene="HACE1"
                     /gene_synonym="SPPRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1094..1200
                     /gene="HACE1"
                     /gene_synonym="SPPRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1201..1351
                     /gene="HACE1"
                     /gene_synonym="SPPRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1352..1686
                     /gene="HACE1"
                     /gene_synonym="SPPRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1687..1755
                     /gene="HACE1"
                     /gene_synonym="SPPRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1756..1843
                     /gene="HACE1"
                     /gene_synonym="SPPRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1844..1955
                     /gene="HACE1"
                     /gene_synonym="SPPRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1956..2053
                     /gene="HACE1"
                     /gene_synonym="SPPRS"
                     /inference="alignment:Splign:2.1.0"
     exon            2054..2141
                     /gene="HACE1"
                     /gene_synonym="SPPRS"
                     /inference="alignment:Splign:2.1.0"
     exon            2142..2291
                     /gene="HACE1"
                     /gene_synonym="SPPRS"
                     /inference="alignment:Splign:2.1.0"
     exon            2292..2488
                     /gene="HACE1"
                     /gene_synonym="SPPRS"
                     /inference="alignment:Splign:2.1.0"
     exon            2489..2620
                     /gene="HACE1"
                     /gene_synonym="SPPRS"
                     /inference="alignment:Splign:2.1.0"
     exon            2621..2719
                     /gene="HACE1"
                     /gene_synonym="SPPRS"
                     /inference="alignment:Splign:2.1.0"
     exon            2720..2790
                     /gene="HACE1"
                     /gene_synonym="SPPRS"
                     /inference="alignment:Splign:2.1.0"
     exon            2791..2904
                     /gene="HACE1"
                     /gene_synonym="SPPRS"
                     /inference="alignment:Splign:2.1.0"
     exon            2905..4575
                     /gene="HACE1"
                     /gene_synonym="SPPRS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4555..4560
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HACE1"
                     /gene_synonym="SPPRS"
                     /note="hexamer: AATAAA"
     polyA_site      4575
                     /gene="HACE1"
                     /gene_synonym="SPPRS"
                     /note="major polyA site"
ORIGIN      
        1 agaaggccac gcgagcccgg gagggacgcg gcggcggggg ctgcaggaaa ggcgcgagca
       61 gaggcggcgg cgggtgtact gtaggtggtc ggtccggcag cagcccggcc cccggacgca
      121 ggacgtggcc ccaggcagcc ctcgcagctc agtgctctag ccggggcaag cccgcgtctc
      181 cgcctgctgg acgggcccag gcgagatgta gggctctggg cgcggaggcc gccggtgggg
      241 cggctgatcg cggaggatcg cggagggcgc gccgaggatg gagagagcga tggagcaact
      301 caaccgcctg acgcgctcgc tgcgccgcgc gcgcaccgtg gagttgcccg aggataatga
      361 aactgctgtt tatacattaa tgccaatggt tatggctgat caacacaggt ctgtttctga
      421 actactatca aattcaaaat ttgatgtcaa ttatgcattc ggacgtgtga aaagaagctt
      481 gcttcacatt gcagcaaatt gtggatcggt ggaatgcttg gttttgctgt taaagaaagg
      541 agcaaatcct aactatcaag atatttcagg ctgtacaccc cttcatttgg cagcaagaaa
      601 tgggcagaag aaatgtatga gtaaattatt agaatatagc gctgatgtca acatttgtaa
      661 taatgaaggc cttacagcaa tacattggct ggctgtgaat gggcggacag aactactcca
      721 tgaccttgtg cagcatgtca gtgatgttga tgttgaggat gccatggggc agacagcact
      781 gcatgttgcc tgccagaacg gtcacaagac gacagtgcag tgcttgctag acagtggtgc
      841 tgatattaac aggccaaatg tatcaggagc aactccattg tactttgctt gcagtcatgg
      901 tcagagagat acagcacaga tcctactatt acgaggagcc aaatatctgc cagataaaaa
      961 tggagtaact cctctggatt tatgtgtaca gggtggatat ggagagactt gtgaagtatt
     1021 aattcaatat cacccgaggc tttttcagac tattattcaa atgacacaga atgaagacct
     1081 ccgagaaaac atgttacggc aagttctgga gcatttgtct cagcaaagtg aaagccagta
     1141 cctaaagatt ctaacaagcc ttgctgaagt tgctacaaca aatggtcata aactgcttag
     1201 cctctctagc aattatgatg ctcaaatgaa gagcctttta aggattgtga gaatgttttg
     1261 tcacgtcttt cgaattggtc catcctcccc cagtaatgga attgatatgg gctacaatgg
     1321 gaataaaact ccaagaagcc aggtgttcaa gcctctggaa ttgctttggc actcgttaga
     1381 tgaatggcta gttttaatag ccacagaatt gatgaaaaac aaaagagact caacagagat
     1441 cacttctatt ttactgaaac aaaaaggcca agatcaagat gctgcttcca ttcctccatt
     1501 tgaacctcca ggacctggga gctatgaaaa tctgtccact ggcacaaggg aatctaaacc
     1561 agatgctctt gcagggagac aggaagccag tgcagattgt caggatgtta tttctatgac
     1621 agctaaccgg ctaagtgctg tcattcaagc tttttacatg tgctgttctt gtcagatgcc
     1681 tccgggaatg acttcacctc gtttcattga atttgtctgc aaacatgatg aagttttaaa
     1741 atgctttgtt aatagaaatc ccaaaattat atttgaccac tttcactttc tccttgaatg
     1801 tcctgagttg atgtcaagat tcatgcatat cataaaagca cagcctttta aagatcgctg
     1861 tgaatggttc tatgaacatt tgcattcagg acagccagat tcagatatgg tgcacaggcc
     1921 agtgaatgaa aatgatatcc tgctggttca cagagattct atttttagga gtagctgtga
     1981 agttgtgtca aaagcaaatt gtgcaaagct aaagcaaggg attgctgtac ggttccatgg
     2041 agaagaaggc atgggtcaag gtgttgtgcg tgagtggttt gatattctgt ccaatgagat
     2101 agtcaatcct gattatgcat tgtttaccca gtcagctgat ggaacaactt ttcagcctaa
     2161 tagcaactct tatgtaaatc ctgatcactt gaactatttt cggtttgctg ggcagatctt
     2221 gggattagcg ttgaaccaca ggcagctggt caatatttac ttcacacgat ccttctacaa
     2281 gcacattctt ggtattcctg taaattacca agatgtggca tccattgatc cagaatatgc
     2341 gaaaaatttg caatggattt tagataatga tataagtgat ctgggtctag aactaacttt
     2401 ttctgttgag actgatgtgt ttggagcaat ggaagaggtg cctttgaaac ctgggggtgg
     2461 gagtattctt gtgacacaaa ataataaagc ggagtacgtc cagcttgtta ctgaacttcg
     2521 aatgacaaga gccattcagc ctcagatcaa tgctttttta cagggctttc atatgttcat
     2581 tccaccctcc ctcatacagc tttttgatga atatgaattg gagctactgc tttctggcat
     2641 gccagaaatt gatgtgagtg attggataaa aaatacagaa tacacaagtg gctatgaaag
     2701 agaagatcca gttattcagt ggttctggga agttgtagaa gacattactc aagaggagag
     2761 agttcttctc ttacagtttg ttacgggcag ttccagggtc ccacatggtg ggtttgctaa
     2821 tatcatgggt ggaagtggat tgcaaaactt tacaatcgct gctgtgccat atactccaaa
     2881 tcttttacca acttcaagca catgcatcaa catgctcaag ttacctgaat acccaagtaa
     2941 agaaatactc aaggacagac ttcttgtggc actacattgt ggcagctatg gttacacaat
     3001 ggcataatga agtctggaaa actcctctga ctactgatgc acaattcaga atggcagaag
     3061 taatttggga aaatgtcaac aaaaaagcag cctaaatgca acccataggc agggctgatg
     3121 cttccaattt ataaaggatc atcaggtttt ctgtttctct cttttccctt ttatgttttc
     3181 tctgtttgtg atacaattag aaaatataaa atcacagtag attttatttt ttaaaatgct
     3241 aactgaaagt aatagagact gtcctttttc ataattaatt ttatccaaga ttgtattaag
     3301 gcaaaatctg attctacatt ccacctctgc tatgtaactg tcttgttaaa agggtgtttt
     3361 ctcctaattt ctgatatatt atatgaggtc atccagctgg tgtgttcttt tgcatgtaaa
     3421 ctgccattta tattttagaa aactattgta tagaatggat ttagattgtc tataaagcca
     3481 caaatacgta ttttgccaca gtgtattcta tattgcaatg atttttttag cattttaata
     3541 ttttaatata tattgtaaaa tttagactga tgatactaac agttgatgaa atgacatata
     3601 atttatatat gaaagcttac gctatattgt atgaattatt tgcatctttc agtggccagt
     3661 tttccatatg tatatattat ggtctcaatg tttttcttac gcctcatttt aatttataat
     3721 gaaggtaaaa ttaaaatgta ttttaccacg tttcttttca ttacttttat ctgtgagctc
     3781 tgacacatct gaaaaagtaa tctgatgtgc aaattataat ttaaatatgt taattttttt
     3841 gcttcttaaa tttgcttttc atcattaaaa tgtcaagttc aagtgatatg tgcctaatat
     3901 cacttggatg ttggtgggtt tttgaatttt tgggtggtta atcagtttta ttttgaaaag
     3961 acgtacttga atagttacag catatgtttg aacaggaagt aggaacatgc atacacgaag
     4021 aaatgctaac ggaaggattt gttatgttta ggatcttccc ttggaaacta aaaatagaat
     4081 attaatgaca ttactgtttg tagaatgaca tatgcagatt ttctcataag cagtcattgt
     4141 gtttgccagt aatgtttgag agacatgtaa gttgaaagtt ttgctaaatt ataaagctcc
     4201 tttaattcgt tggttttgat tctcttattc tcttgtcttt tctaaatgtt aacaaaatat
     4261 atcttaacag attacatgaa atttaggaat tatttaaaag ttaccattag ctctaaaatt
     4321 aagattcgga tgctttattt atagtaactg aagctaataa tgttttatgt tttgattttt
     4381 tgaaatttaa ttgtagaagt cactgccttc tgagttttca aatagataac cacctttaat
     4441 attacactgc ttataatact aatgtttaca gatatgtttc tgtttataac catataatac
     4501 attggctttg tcatattagt tttttttgca agtagttatg taaaagagat agataataaa
     4561 atattaaata actga
//
