LOCUS       NM_001010915            8277 bp    mRNA    linear   PRI 01-JUN-2024
DEFINITION  Homo sapiens 3-hydroxyacyl-CoA dehydratase 4 (HACD4), transcript
            variant 1, mRNA.
ACCESSION   NM_001010915 XM_376819
VERSION     NM_001010915.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8277)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 8277)
  AUTHORS   Jo,Y.S., Kim,M.S., Yoo,N.J. and Lee,S.H.
  TITLE     Inactivating Frameshift Mutations of HACD4 and TCP10L Tumor
            Suppressor Genes in Colorectal and Gastric Cancers
  JOURNAL   Pathol Oncol Res 26 (1), 583-584 (2020)
   PUBMED   29532408
  REMARK    GeneRIF: Inactivating Frameshift Mutations of HACD4 and TCP10L
            Tumor Suppressor Genes in Colorectal and Gastric Cancers.
REFERENCE   3  (bases 1 to 8277)
  AUTHORS   Zivotic,I., Djuric,T., Stankovic,A., Ivancevic,I., Koncar,I.,
            Milasinovic,D., Stankovic,G., Alavantic,D. and Zivkovic,M.
  TITLE     The HACD4 haplotype as a risk factor for atherosclerosis in males
  JOURNAL   Gene 641, 35-40 (2018)
   PUBMED   29031776
  REMARK    GeneRIF: association of HACD4 haplotypes with atherosclerotic
            phenotypes connotes a further validation and replication in larger
            cohorts as well as functional studies to enlighten the potential
            mechanism of its action in pathophysiology of atherosclerosis.
REFERENCE   4  (bases 1 to 8277)
  AUTHORS   Zhu,S., Wang,Z., Zhang,Z., Wang,J., Li,Y., Yao,L., Mei,Q. and
            Zhang,W.
  TITLE     PTPLAD2 is a tumor suppressor in esophageal squamous cell
            carcinogenesis
  JOURNAL   FEBS Lett 588 (6), 981-989 (2014)
   PUBMED   24530685
  REMARK    GeneRIF: PTPLAD2 is a potential tumor suppressor and prognostic
            indicator that reduces STAT3 phosphorylation.
REFERENCE   5  (bases 1 to 8277)
  AUTHORS   Cheng,C.Y., Schache,M., Ikram,M.K., Young,T.L., Guggenheim,J.A.,
            Vitart,V., MacGregor,S., Verhoeven,V.J., Barathi,V.A., Liao,J.,
            Hysi,P.G., Bailey-Wilson,J.E., St Pourcain,B., Kemp,J.P.,
            McMahon,G., Timpson,N.J., Evans,D.M., Montgomery,G.W., Mishra,A.,
            Wang,Y.X., Wang,J.J., Rochtchina,E., Polasek,O., Wright,A.F.,
            Amin,N., van Leeuwen,E.M., Wilson,J.F., Pennell,C.E., van
            Duijn,C.M., de Jong,P.T., Vingerling,J.R., Zhou,X., Chen,P., Li,R.,
            Tay,W.T., Zheng,Y., Chew,M., Burdon,K.P., Craig,J.E., Iyengar,S.K.,
            Igo,R.P. Jr., Lass,J.H. Jr., Chew,E.Y., Haller,T., Mihailov,E.,
            Metspalu,A., Wedenoja,J., Simpson,C.L., Wojciechowski,R., Hohn,R.,
            Mirshahi,A., Zeller,T., Pfeiffer,N., Lackner,K.J., Bettecken,T.,
            Meitinger,T., Oexle,K., Pirastu,M., Portas,L., Nag,A.,
            Williams,K.M., Yonova-Doing,E., Klein,R., Klein,B.E.,
            Hosseini,S.M., Paterson,A.D., Makela,K.M., Lehtimaki,T.,
            Kahonen,M., Raitakari,O., Yoshimura,N., Matsuda,F., Chen,L.J.,
            Pang,C.P., Yip,S.P., Yap,M.K., Meguro,A., Mizuki,N., Inoko,H.,
            Foster,P.J., Zhao,J.H., Vithana,E., Tai,E.S., Fan,Q., Xu,L.,
            Campbell,H., Fleck,B., Rudan,I., Aung,T., Hofman,A.,
            Uitterlinden,A.G., Bencic,G., Khor,C.C., Forward,H., Parssinen,O.,
            Mitchell,P., Rivadeneira,F., Hewitt,A.W., Williams,C., Oostra,B.A.,
            Teo,Y.Y., Hammond,C.J., Stambolian,D., Mackey,D.A., Klaver,C.C.,
            Wong,T.Y., Saw,S.M. and Baird,P.N.
  CONSRTM   Consortium for Refractive Error and Myopia; Fuchs' Genetics
            Multi-Center Study Group; Wellcome Trust Case Control Consortium 2;
            Diabetes Control and Complications Trial/Epidemiology of Diabetes
            Interventions, and Complications Research Group
  TITLE     Nine loci for ocular axial length identified through genome-wide
            association studies, including shared loci with refractive error
  JOURNAL   Am J Hum Genet 93 (2), 264-277 (2013)
   PUBMED   24144296
REFERENCE   6  (bases 1 to 8277)
  AUTHORS   Ikeda,M., Kanao,Y., Yamanaka,M., Sakuraba,H., Mizutani,Y.,
            Igarashi,Y. and Kihara,A.
  TITLE     Characterization of four mammalian 3-hydroxyacyl-CoA dehydratases
            involved in very long-chain fatty acid synthesis
  JOURNAL   FEBS Lett 582 (16), 2435-2440 (2008)
   PUBMED   18554506
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            HY118500.1, BX648779.1, BC114215.1 and AL662879.5.
            
            On Nov 22, 2018 this sequence version replaced NM_001010915.4.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803611.149241.1,
                                           SRR1803617.120925.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000495827.3/ ENSP00000419503.1
            RefSeq Select criteria :: based on expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-4                 HY118500.1         36-39
            5-190               BX648779.1         2-187
            191-1420            BC114215.1         160-1389
            1421-8277           AL662879.5         70034-76890
FEATURES             Location/Qualifiers
     source          1..8277
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9p21.3"
     gene            1..8277
                     /gene="HACD4"
                     /gene_synonym="PTPLAD2"
                     /note="3-hydroxyacyl-CoA dehydratase 4"
                     /db_xref="GeneID:401494"
                     /db_xref="HGNC:HGNC:20920"
                     /db_xref="MIM:615941"
     exon            1..88
                     /gene="HACD4"
                     /gene_synonym="PTPLAD2"
                     /inference="alignment:Splign:2.1.0"
     CDS             51..749
                     /gene="HACD4"
                     /gene_synonym="PTPLAD2"
                     /EC_number="4.2.1.134"
                     /note="isoform 1 is encoded by transcript variant 1;
                     protein tyrosine phosphatase-like protein PTPLAD2;
                     very-long-chain (3R)-3-hydroxyacyl-[acyl-carrier protein]
                     dehydratase 4; very-long-chain (3R)-3-hydroxyacyl-CoA
                     dehydratase 4; protein tyrosine phosphatase-like A domain
                     containing 2; protein-tyrosine phosphatase-like A
                     domain-containing protein 2"
                     /codon_start=1
                     /product="very-long-chain (3R)-3-hydroxyacyl-CoA
                     dehydratase 4 isoform 1"
                     /protein_id="NP_001010915.2"
                     /db_xref="CCDS:CCDS43791.1"
                     /db_xref="GeneID:401494"
                     /db_xref="HGNC:HGNC:20920"
                     /db_xref="MIM:615941"
                     /translation="MGPLALPAWLQPRYRKNAYLFIYYLIQFCGHSWIFTNMTVRFFS
                     FGKDSMVDTFYAIGLVMRLCQSVSLLELLHIYVGIESNHLLPRFLQLTERIIILFVVI
                     TSQEEVQEKYVVCVLFVFWNLLDMVRYTYSMLSVIGISYAVLTWLSQTLWMPIYPLCV
                     LAEAFAIYQSLPYFESFGTYSTKLPFDLSIYFPYVLKIYLMMLFIGMYFTYSHLYSER
                     RDILGIFPIKKKKM"
     exon            89..192
                     /gene="HACD4"
                     /gene_synonym="PTPLAD2"
                     /inference="alignment:Splign:2.1.0"
     exon            193..320
                     /gene="HACD4"
                     /gene_synonym="PTPLAD2"
                     /inference="alignment:Splign:2.1.0"
     exon            321..433
                     /gene="HACD4"
                     /gene_synonym="PTPLAD2"
                     /inference="alignment:Splign:2.1.0"
     exon            434..540
                     /gene="HACD4"
                     /gene_synonym="PTPLAD2"
                     /inference="alignment:Splign:2.1.0"
     exon            541..666
                     /gene="HACD4"
                     /gene_synonym="PTPLAD2"
                     /inference="alignment:Splign:2.1.0"
     exon            667..8277
                     /gene="HACD4"
                     /gene_synonym="PTPLAD2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      805..810
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HACD4"
                     /gene_synonym="PTPLAD2"
                     /note="hexamer: AATAAA"
     polyA_site      836
                     /gene="HACD4"
                     /gene_synonym="PTPLAD2"
                     /note="major polyA site"
     regulatory      1077..1082
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HACD4"
                     /gene_synonym="PTPLAD2"
                     /note="hexamer: ATTAAA"
     polyA_site      1100
                     /gene="HACD4"
                     /gene_synonym="PTPLAD2"
     regulatory      4144..4149
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HACD4"
                     /gene_synonym="PTPLAD2"
                     /note="hexamer: AATAAA"
     polyA_site      4165
                     /gene="HACD4"
                     /gene_synonym="PTPLAD2"
     regulatory      8247..8252
                     /regulatory_class="polyA_signal_sequence"
                     /gene="HACD4"
                     /gene_synonym="PTPLAD2"
                     /note="hexamer: AATAAA"
     polyA_site      8277
                     /gene="HACD4"
                     /gene_synonym="PTPLAD2"
ORIGIN      
        1 ccccggtact ccttcctgga ccgcgctgga agccctggcg gcggcggccc atggggccct
       61 tggcgctgcc cgcctggctg cagcccaggt ataggaagaa tgcgtatctt ttcatctatt
      121 acttaatcca gttctgtggc cactcttgga tatttacaaa tatgacagtc agattctttt
      181 catttggaaa agattcaatg gttgacactt tttatgctat tggacttgtg atgcgacttt
      241 gccaatccgt atctctcctg gaactgctgc acatatatgt tggcattgag tcaaaccatc
      301 ttctcccaag gtttttgcag ctcacagaaa gaataatcat cctttttgtg gtgatcacca
      361 gtcaagagga agtccaagag aaatatgtgg tgtgtgtttt attcgtcttt tggaatctat
      421 tggatatggt taggtacact tatagcatgt tatcagtcat aggaatatcc tatgctgtct
      481 tgacatggct cagtcaaaca ctatggatgc caatttatcc tttgtgtgtt cttgctgaag
      541 catttgccat ctatcaatcg ctgccttatt ttgaatcatt tggcacttat tccaccaagc
      601 tgccctttga cttatccatc tatttcccat atgtgctgaa aatatatctc atgatgctct
      661 ttataggtat gtattttacc tacagtcatc tatactcaga aagaagagac atcctcggaa
      721 tctttcccat taaaaaaaag aagatgtgaa gtacagcatt ccagtgtgac acgagaaaag
      781 acaggctgtg gattcagtgc agtaaataaa acacaggaag tattctggtg gaaaaatacc
      841 ttgtatttga taaaaatcca ttacatgaaa taactgcaca tacccccaaa actctagaca
      901 taacaggcta tgtttttcac acattgtatt tttaagacat atatattctc cataccctgc
      961 ttcaccaaaa ttcctgcttc atttggattt tatacaagtt atacttacat tgtatgaagt
     1021 ctgattattt tcctgtaagt ttaatgagaa ataggagatt agctgcaata gattcaatta
     1081 aattaagaaa ccaaacttga taacctaaat tacatgtatt ctttttcact tccaagttcc
     1141 caaatagatc tgtaggagaa gagggatgga aaatatattc cacagttgcc tagaataaca
     1201 tatttcatag acagatgtct gtcttcagat ctaataatac tcaccctaaa cattagatat
     1261 gtttagaaaa ttaaatatct ttccaagccc tatgtcttag ctcaggttgc tataatagaa
     1321 caccataaac cgaatggctt aaacaacagc aaacgcttct cacagttctg gaggctggga
     1381 attccgctca ggatcaaggc atcagctaat gcagtttctg gtgagggccc tcttcccagt
     1441 ttgcagatag atgtccttct ttgtcctcac atgagagaga gatcaactct cttatgtctc
     1501 ttataatggc actaacccca ttcatgaggg ctctaccctc atgatctaat tacctcccaa
     1561 tggccccacc tccaaatacc atcatattgg aggttagggc ttaacacatg aattttgggg
     1621 gaacacaaag attcactcca tagcattctg tacctggtcc tacaaaattc atgtcctcct
     1681 cacatgcaaa atacatacat tccatctcga cagcctcaaa agtcttaact cattcctgca
     1741 tcaactctaa agtccaaagt ctcgtctaaa tcagatgtgg gtgagattca aggtagaatt
     1801 catcctgagg taaaattcct ttccagcttt gaacttgtga aatgaaacaa gttatgtgct
     1861 tccacagtac aatggtggga cacacaaagg gtggacattc ctattcccaa agcaagaaac
     1921 aggaaaaaag gaaggtgtaa tgggtcccca gcaagtccaa aacctagcaa ggcaaacacc
     1981 atgagatctt cagtcttagt aataatcctc tttggcctga tactctggct tcaggaccca
     2041 gtgaggcaga ggtattgccc ccaggactat gttggaggag gatgaccccc atatcaccag
     2101 gcggaagctg tcaggcctgt tgaaaccaag gcaatgactg ccccttttga aactgagaag
     2161 gtaccactga tgttctctga atcattgttc cgggtcagtt tctccttttc ttgaagaata
     2221 gtgtacattc acagctgaat agctctatgg tcccaaattc aagaagtcca acagtcttcc
     2281 ctcattctgt cccatctcca ttcctttcaa tacaaactag cagtgttcct gctggggtgg
     2341 ctgattaggt ctgtggttca cacccacata aatctaattg gatgacttgt ctgctacact
     2401 cttagtgttc tctaccaaac gtgttttctt atctttttgt aatgcagaca gactgataat
     2461 ttcccacatc ttttaagttc tggttccgtt tttgttaaga attctgtctt cattttttgt
     2521 cacattttac tttaagcagt caagataaac cgaggcactc cttcaacact ggttggagat
     2581 ctcttcagtt caatatctaa tttcatggct cacaatttta ctttccacaa aacactagaa
     2641 catgaacaca atcatccaag ttctttggca tattataaca aagatcacct ttcttccagt
     2701 ttcccataac aggttcctca ttttcatttg agacttcatc agaatggcct ttattgtcca
     2761 tgtctaccaa cattatcttc atggtgattt acgtgttctc tatgaagatg gaggctttct
     2821 ctcccaccct cttctctttg agccctcacc aaaatcacct ctatcaatcc cttcatggca
     2881 atactggctt tttctagcaa aaaaactagt acctcaaaac tcttccagta ctcttccagg
     2941 tactctagta cctcaaaact cttccagctt ctatctgtta cctaacgtca aagctgcttc
     3001 cacatcttta tatattggtt atagcatcac cacacttctt ggtactaatt gctgtcttag
     3061 cttggctgct ataacaaaat accataaact gggtggctta gacaacaaat atatttctca
     3121 cagttctgga ggcagggaag tccaagataa aggtgctaga caattaagtt cctgatgagg
     3181 ttccaaggtg ctagctgatt cttcttcatg gtttgcagat gaatgccttc ttgctatatg
     3241 ttcacatggt ggagggagaa accatctctc ccttatttct tattgtaaag gtactaatcc
     3301 catttatgaa ggttccagct tcaaatgcca tcacactgga gattagattt tcaacatgtg
     3361 aatttaatgg gggacaccag cattcagtcc ctagtgctct gatagagtat tcattaatgt
     3421 catgtatttg ttaacatctt gtaagttatt tgggctttga tgccttaaaa gaagaccctg
     3481 gccaggcatg ctggctcata cctgtaatcc tagcactatg agaggctgag gtgggcagat
     3541 cagttgaggt caggagttca agaccagcct ggccaacatg gtgaaacccc atttctacta
     3601 aaaatacaaa aattagccag gcatggtggc gcatgcctgt aatcccagct acacgggagg
     3661 ctgggggcat gagaatcgct tgagcccagg agatggaggt tgcagtgagc agagattgag
     3721 ccactgcact ccagcctggg tgacagagtg agactctatc tcaaaagaga agaccctaat
     3781 gaaaatagta aacctttagc aaatacacaa atgatgcccc attctgtatt ctttttaatt
     3841 ttaaaaataa tgacacacac atacaggaag ataaaaggta taaaaatata ctaagtactt
     3901 tttaaaaagg aaaattcatg ttttggatta ttactgaatt gttgctaatt actgtaatgt
     3961 aagtcatatg tccttacata gttttgaaaa atgctttctt tgaaaatcta aaaaaagtgt
     4021 gaataactga tagtcattgc tgtatcattg atgtatcact caatttttgg taaagaatta
     4081 taaagatctt acctagaaac tatgtaaaaa ggtattaaat atattgtgat gtaagaatag
     4141 gataataaaa atgtaatttt aaaaatgggt aacttatttc cactaaagaa aacatttgga
     4201 aagaaacaat tgagtattag gctacttgaa atctttataa atatatttgg attttaaaaa
     4261 attatccttt taaaaagcta cattttttct gcttttatag ttatcttcta aatttcagaa
     4321 aaattatgaa aagctttagt gtgcaaggag aacataaacc ctttgcaaat tttaggtgag
     4381 ttaatcttaa tcactccaaa taaagatggc tccttacact gccgaagtac atgtacaatc
     4441 aatcactaaa acaaagagaa aattagagaa actacacttt actgggtcat tataataaat
     4501 taaaatttca ttattaaata atgctttaat tttttaaaac cagattaact taaaaacttc
     4561 ttaaaattga aaaccaaaag aaacacaagt gaatttataa ttattttaaa acagaaatga
     4621 ggattgacag taatttgaga tatctgcaac aattgtaaca taaaaagatc ttacatttcc
     4681 tttggtgaca gacacaattg ttaatactac tgtggttggt tgcctgtatt cacaatggaa
     4741 aggaatacta aatttcatta tcaattagtg aaaataaagg tgtaatttct ttttctactt
     4801 aagtacatgt tccccaggtt aagaaaccca gacatagtga agtcaagaca gtaactatct
     4861 ggttaatcgg atgttaccaa aatcgcaaag ttgatgtctt tgactatctg tcttaactgt
     4921 atgttcacca aatccctatt tcattttgta cagtgtagac aaaggcttac tttctcaaat
     4981 ctaagtaaca tagatgaaat aggaaccatt agatattttg aaaacatatc taagacaact
     5041 tttttcagta atttttttat tgtggtaaaa tgtaaaatat actatcttat tcctttttaa
     5101 gtgtgcagtt cagtagtagt aagtaattta tattgtgcaa ccatcaccac tgtccatctc
     5161 cagaactttt tttaccttgc aaaacttata ccctatactc attaagcaat ggttccctat
     5221 tctcccatct gccctgtccc tggcaaccac cagtctactt tttgtctcta tgattttgat
     5281 tgctctaagt acctcatata aatgtaatca tacagtatct gtctttcgta attggcttat
     5341 ttcacttagc ataatgtctg caagtttcct ccattttgta tatgtcagaa ttttcttcct
     5401 ttttgaggat gactgataag gagggactta ctcctgtcat tttgctattt gttttctaca
     5461 tagcttagct tttttgtccc tcatttcctg cattacgtct tttgtgtgta gtttactttt
     5521 ttatagtgaa gcatttaagt atctttctca gttcttttgt gtatattctg tagctgtttt
     5581 ctttgtggtt accatagggt gacatttaat atccggaagg taatttggac ttatgccagc
     5641 tgaacttcaa aaatatacaa aaattctgct ctttatatct ctgtctatca ctcctttaag
     5701 tttcacaaaa ttatatacac tgtgtgtcca aaatcaattt ttaaaaatgc agtagtctct
     5761 atgtagaaaa caaaaagtgg agttacaaac cattgttaca aaagtactag attttataat
     5821 tgcccatgta tttttcttta ctgagatcct tatttcttca tgtggctttg agttgctgtt
     5881 tagtgtcttt ccatttcaac ttgcaggacc cctattagca tttctggcag gggcaggtct
     5941 agtggtaaca tactttctca gcttttgttt atctggggat gtcttaattt ctctctcacg
     6001 tttgaagaac agttttgtca aatacaggaa atgtggttga tgatatattt ttttcctaac
     6061 actttgaata tgtgagctca ctgcattctg gcctccaaag tttctgatga gaaatctgat
     6121 aatcttatta aggatcactt atatgtgatg aattgatttt ttcatgctac tttcaagatt
     6181 ctctttctgt ctttggcttt tgatagttgt gtggatctct tggaattcat tctacttgga
     6241 gtttgttggt cattatctgg ctgttcttca aatattctct ctgctccttt ctttctctct
     6301 tttccttctg ggatcctcac aatgtgtatg ttggtctgct tgatgggtcc cacagatcct
     6361 tataccctgt tcactttttt ccaatttttt tctattgctc agactaataa tttccattgt
     6421 cctattttca tgtatactga ttcttctgct gcccaaatct gcctttgaat acctctagtg
     6481 agtttttcat ttcaattatc gtactttcca gctcttgaat ttcttttttg tatcgtctta
     6541 gattttctct atattgatat taccattttg ttcatccatt ggttttttga ctttttccgc
     6601 atgctctttg gttctttaaa aaacatcctt aaggcagtcg ttgtagagcc tttagtggat
     6661 ccaccatcaa gtctttttca gagatagttt ttgtttttgt tttcatttga aagggacata
     6721 ctttcctgtt tctttgtatg ccttgttatt tttttgctga aaactgaacg tttcaatcta
     6781 ataatatggt aactacatct cactaaggtc cctaagttaa tgtttttttt taaactattc
     6841 cttactttgc ctgccttgag caagttgtca aatgtgagaa actgtattta tttcagaagt
     6901 acaaatagat acaatctcaa tgtccaaaat atttccccaa agaaagaatc ttacaattat
     6961 ttaagtttac ttatggagaa aacattttat ggtactaaag tgttaaagtg atttaaaatt
     7021 aaggttaaaa tatgctaaaa attatcttcc tttacccagt atctatcttc cacttgttta
     7081 ttactgcttt ttaataatta taatgtttcc cgttggttcg gataatttta atattcttct
     7141 cggtctgagt tatatgctaa tgttgatatg acatggttct ccagggtagt gttcagattg
     7201 tatgttcagg gagcaacagc cacaattaac aagttcacct gtttagaatg cagattccag
     7261 ggctccacta aagatagttg aatcagaagc tctgggaata agctcaggat cctacctttt
     7321 gatagactcc tcaaatgagc ctcatgcaca gaaaagtttg agaaccactg cttttgagtc
     7381 taaattattt cagctttgga cagaaagaaa actgctaaaa agaactactt atataaaagc
     7441 caccaatgtt ttcagaacag ggatgatttt ttatggcttt aaaaatcctt aaatagtagt
     7501 tctgaacatg tttttaacat gccaagtaag aattactaac tagtttattt ttgctaaggg
     7561 tagctggagt tttaaatctc atctaaaata aggttagtta attccatatg aaagtcaatt
     7621 agtaaaagta tgccttaatt catcacatac atgtaggtgt ctattgttac ttcatggtat
     7681 tcagctgaat gatggggttc ttgtgaaaga taatgtggtc tggaattaag agcactaggt
     7741 ttgatttgag atcaagctcc attatttact aactgaatga ctctttgagt tccagtttcc
     7801 ttaaccactt aaagttatta tgaagactaa atgacatatg gtaaattaaa cttttgcaaa
     7861 ttataaaggg ctttgcagaa gtaggacatt tataacatgt tctgttctta cttcatgatg
     7921 gtttagtgat ttatattctc tgactgtaag ttccatgaga gcagggatga cacgttttgt
     7981 tcagattgta ccccagaaac ttgtatagtg gcacataata gacacccata attgtgtgtg
     8041 caactctatc tttaaagaac gccactacta catctagaaa tatatttcaa gaaatctatc
     8101 ggcttaatta ggtaatgaat ttcaattgta tctcaaagcg ttccttatat ttagaagaca
     8161 cataagtttt gtttttttct ttcaacttat ataagtatct taacatgcta cttctagaat
     8221 attttaattg ttaagaacaa tttcttaata aacttccact gtcttcctga gtcccca
//
