LOCUS       NM_003528               2224 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens H2B clustered histone 21 (H2BC21), mRNA.
ACCESSION   NM_003528
VERSION     NM_003528.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Chen,S., Dai,X., Li,H., Gong,Y., Zhao,Y. and Huang,H.
  TITLE     Overexpression of ring finger protein 20 inhibits the progression
            of liver fibrosis via mediation of histone H2B lysine 120
            ubiquitination
  JOURNAL   Hum Cell 34 (3), 759-770 (2021)
   PUBMED   33575967
  REMARK    GeneRIF: Overexpression of ring finger protein 20 inhibits the
            progression of liver fibrosis via mediation of histone H2B lysine
            120 ubiquitination.
REFERENCE   3  (bases 1 to 2224)
  AUTHORS   Zhang,L., Zhang,W., Sun,J., Liu,K.N., Gan,Z.X., Liu,Y.Z.,
            Chang,J.F., Yang,X.M. and Sun,F.
  TITLE     Nucleotide variation in histone H2BL drives crossalk of histone
            modification and promotes tumour cell proliferation by upregulating
            c-Myc
  JOURNAL   Life Sci 271, 119127 (2021)
   PUBMED   33515561
  REMARK    GeneRIF: Nucleotide variation in histone H2BL drives crossalk of
            histone modification and promotes tumour cell proliferation by
            upregulating c-Myc.
REFERENCE   4  (bases 1 to 2224)
  AUTHORS   Baranova,S.V., Dmitrienok,P.S., Buneva,V.N. and Nevinsky,G.A.
  TITLE     HIV-Infected Patients: Cross Site-Specific Hydrolysis of H2a and
            H2b Histones and Myelin Basic Protein with Antibodies against These
            Three Proteins
  JOURNAL   Biomolecules 10 (11), 1501 (2020)
   PUBMED   33143355
  REMARK    GeneRIF: HIV-Infected Patients: Cross Site-Specific Hydrolysis of
            H2a and H2b Histones and Myelin Basic Protein with Antibodies
            against These Three Proteins.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2224)
  AUTHORS   Marzluff,W.F., Gongidi,P., Woods,K.R., Jin,J. and Maltais,L.J.
  TITLE     The human and mouse replication-dependent histone genes
  JOURNAL   Genomics 80 (5), 487-498 (2002)
   PUBMED   12408966
REFERENCE   6  (bases 1 to 2224)
  AUTHORS   Rodriguez,P., Munroe,D., Prawitt,D., Chu,L.L., Bric,E., Kim,J.,
            Reid,L.H., Davies,C., Nakagama,H., Loebbert,R., Winterpacht,A.,
            Petruzzi,M.J., Higgins,M.J., Nowak,N., Evans,G., Shows,T.,
            Weissman,B.E., Zabel,B., Housman,D.E. and Pelletier,J.
  TITLE     Functional characterization of human nucleosome assembly protein-2
            (NAP1L4) suggests a role as a histone chaperone
  JOURNAL   Genomics 44 (3), 253-265 (1997)
   PUBMED   9325046
REFERENCE   7  (bases 1 to 2224)
  AUTHORS   Frohm,M., Gunne,H., Bergman,A.C., Agerberth,B., Bergman,T.,
            Boman,A., Liden,S., Jornvall,H. and Boman,H.G.
  TITLE     Biochemical and antibacterial analysis of human wound and blister
            fluid
  JOURNAL   Eur J Biochem 237 (1), 86-92 (1996)
   PUBMED   8620898
REFERENCE   8  (bases 1 to 2224)
  AUTHORS   Jackson,S., Brooks,W. and Jackson,V.
  TITLE     Dynamics of the interactions of histones H2A,H2B and H3,H4 with
            torsionally stressed DNA
  JOURNAL   Biochemistry 33 (18), 5392-5403 (1994)
   PUBMED   8180162
REFERENCE   9  (bases 1 to 2224)
  AUTHORS   Collart,D., Romain,P.L., Huebner,K., Pockwinse,S., Pilapil,S.,
            Cannizzaro,L.A., Lian,J.B., Croce,C.M., Stein,J.L. and Stein,G.S.
  TITLE     A human histone H2B.1 variant gene, located on chromosome 1,
            utilizes alternative 3' end processing
  JOURNAL   J Cell Biochem 50 (4), 374-385 (1992)
   PUBMED   1469070
REFERENCE   10 (bases 1 to 2224)
  AUTHORS   Collart,D., Ramsey-Ewing,A., Bortell,R., Lian,J., Stein,J. and
            Stein,G.
  TITLE     Isolation and characterization of a cDNA from a human histone H2B
            gene which is reciprocally expressed in relation to
            replication-dependent H2B histone genes during HL60 cell
            differentiation
  JOURNAL   Biochemistry 30 (6), 1610-1617 (1991)
   PUBMED   1993178
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC239868.2.
            
            On Dec 3, 2019 this sequence version replaced NM_003528.2.
            
            Summary: Histones are basic nuclear proteins that are responsible
            for the nucleosome structure of the chromosomal fiber in
            eukaryotes. Two molecules of each of the four core histones (H2A,
            H2B, H3, and H4) form an octamer, around which approximately 146 bp
            of DNA is wrapped in repeating units, called nucleosomes. The
            linker histone, H1, interacts with linker DNA between nucleosomes
            and functions in the compaction of chromatin into higher order
            structures. This gene encodes a replication-dependent histone that
            is a member of the histone H2B family, and generates two
            transcripts through the use of the conserved stem-loop termination
            motif, and the polyA addition motif. The protein has antibacterial
            and antifungal antimicrobial activity. [provided by RefSeq, Aug
            2015].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript is intronless :: BC069193.1, DA559488.1 [ECO:0000345]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match                 :: ENST00000369155.4/
                                                  ENSP00000358151.2
            Protein has antimicrobial activity :: PMID: 12860195
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            replication-dependent histone      :: PMID: 12408966
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2224              AC239868.2         169029-171252       c
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q21.2"
     gene            1..2224
                     /gene="H2BC21"
                     /gene_synonym="GL105; H2B; H2B-GL105; H2B.1; H2BE; H2BFQ;
                     H2BGL105; H2BQ; HIST2H2BE"
                     /note="H2B clustered histone 21"
                     /db_xref="GeneID:8349"
                     /db_xref="HGNC:HGNC:4760"
                     /db_xref="MIM:601831"
     exon            1..2224
                     /gene="H2BC21"
                     /gene_synonym="GL105; H2B; H2B-GL105; H2B.1; H2BE; H2BFQ;
                     H2BGL105; H2BQ; HIST2H2BE"
                     /inference="alignment:Splign:2.1.0"
     CDS             43..423
                     /gene="H2BC21"
                     /gene_synonym="GL105; H2B; H2B-GL105; H2B.1; H2BE; H2BFQ;
                     H2BGL105; H2BQ; HIST2H2BE"
                     /note="histone 2, H2be; H2B histone family, member Q;
                     histone H2B.q; histone cluster 2 H2B family member e;
                     histone H2B-GL105; histone cluster 2, H2be"
                     /codon_start=1
                     /product="histone H2B type 2-E"
                     /protein_id="NP_003519.1"
                     /db_xref="CCDS:CCDS936.1"
                     /db_xref="GeneID:8349"
                     /db_xref="HGNC:HGNC:4760"
                     /db_xref="MIM:601831"
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSIYVYK
                     VLKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLL
                     LPGELAKHAVSEGTKAVTKYTSSK"
     regulatory      2201..2206
                     /regulatory_class="polyA_signal_sequence"
                     /gene="H2BC21"
                     /gene_synonym="GL105; H2B; H2B-GL105; H2B.1; H2BE; H2BFQ;
                     H2BGL105; H2BQ; HIST2H2BE"
                     /note="hexamer: AATAAA"
     polyA_site      2224
                     /gene="H2BC21"
                     /gene_synonym="GL105; H2B; H2B-GL105; H2B.1; H2BE; H2BFQ;
                     H2BGL105; H2BQ; HIST2H2BE"
                     /note="major polyA site"
ORIGIN      
        1 acttcttttc ttggctaagc cgcgtttgta ctgtgtctta ccatgcctga accggcaaaa
       61 tccgctccgg cccctaaaaa gggctccaag aaagccgtca ccaaagccca gaagaaagac
      121 ggcaagaagc gcaagcgcag ccgcaaagag agctactcca tctacgtgta caaggtgctg
      181 aagcaggtcc accccgacac cggcatctcg tccaaggcca tgggcatcat gaactccttc
      241 gtcaacgaca tcttcgagcg catcgcggga gaggcttccc gcctggcgca ctacaacaag
      301 cgctccacca tcacatcccg cgagatccag acggccgtgc gcctgctgct gcccggcgag
      361 ctggccaagc acgccgtgtc cgagggcacc aaggcggtca ccaagtacac cagctccaag
      421 tgagtccctg ccgggacctg gcgctcgctc gctcgagtcg ccggctgctt gactccaaag
      481 gctcttttca gagccaccca cctaatcact agaaaagagc ttgttcactt attcccttag
      541 tttcttttca taaagtaagt tattttagtg tgaaggtcat gggaaatggc atacgtagct
      601 ttttaactat ttggaactcg aggtccccag tgcgtcattg gatttgcttt tgaatctaga
      661 gcgtgtcttt actcattgtg ctgcttagcc ttcccaggag tcggttctca attaggctgt
      721 tgggaatccg cctctttacc cgcccccact cccgccccac acgcgccctg gtggctcctt
      781 gggtctgttt cattctaaaa cgaagtggct gagttcggct gtcatttaag agaactccag
      841 gacacaattc agcccgggtt ccgcaaacac tgcgtgacag ctctgtatga ctgacgcttg
      901 gcagcagctt ttgtgtccgg tcaccagttc tgccgtgcga tggggcctcc tgtggatacc
      961 agccgttctg tgtattttgg acgaaggccg cgcagccggg tcccagcctt gtcctgattg
     1021 ggcgacaaga atattcaaaa ttctgcgcct ttttctaatt tgtagatttc agtttccgtc
     1081 gttcactttg agactttgaa attcctattt ctcattttgt tgataatttc tgcatttaat
     1141 ggtctgtgct ttaaatggta acgctacggc cccaggtcac tgcgaggcac ttaccatgta
     1201 gatacgggct caaaagtcac ctctcagaga cctacgtcat ccactcagga attcgcgcct
     1261 ctcatacttg cctgtctcat tttatcttcc ttctagcagc tgtctgaaat tggttcgtct
     1321 gttttcttgt ttatggtatt ctcaagccct tgacagaccg gctagtgtgg ttttcccgtg
     1381 catcttcagc ctggcacatt atggacactt aaatactacg tattgatcta atattgttgg
     1441 gttaattttt ccatcccacc cttttcttaa tcgcttccgt ggatggatga agggtgctgt
     1501 tcatttccat tagatgtatg tgaaggcaca gtgaaaatgg aaatgttctt ggagctactt
     1561 cctcaaaatg tatccttagt cacctcagtg caacagctgg gagggggccg tgttaagatt
     1621 ttttttgcta caaagaggag gtggcaatgg tagatccacc cttatgcttc tcagtttagc
     1681 ataacctctt atggattttc atcaaattca gcgtgttggt cactggaaag agccttttcc
     1741 ttctcctttt cttactctcc cctcatggtg ttcccctctt aaaggagagg agcttttaat
     1801 ttacacttac cacctcattt gcttttctgg aggccatgca atataggcgg gactacagag
     1861 ttaatctcct ttttacaaat gaggccaaga gaagcctcat tggttcacag tcatgcagct
     1921 catactgtcc acccttgtat tctcagatgc aggacaattg cattttagtt ttattttgtg
     1981 gaggtgcaga atatttactc tttctgtcca acccttgatt ctgccgagga agacactgat
     2041 ggtttgatga gtgattcagc tgtttttggc taagggcttt tggagctgat ggcaggggtt
     2101 tgatgaatcc aaatgagctc tagacattat cacagactga atagatctta actgtctcct
     2161 acatgtgtgt tttcaaatgt gtatagatgc tattgttatt aataaagtta ccaattaatt
     2221 taaa
//
