LOCUS       NM_006144                896 bp    mRNA    linear   PRI 08-OCT-2024
DEFINITION  Homo sapiens granzyme A (GZMA), mRNA.
ACCESSION   NM_006144
VERSION     NM_006144.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Rasi,V., Phelps,K.R., Paulson,K.R., Eickhoff,C.S., Chinnaraj,M.,
            Pozzi,N., Di Gioia,M., Zanoni,I., Shakya,S., Carlson,H.L.,
            Ford,D.A., Kolar,G.R. and Hoft,D.F.
  TITLE     Homodimeric Granzyme A Opsonizes Mycobacterium tuberculosis and
            Inhibits Its Intracellular Growth in Human Monocytes via Toll-Like
            Receptor 4 and CD14
  JOURNAL   J Infect Dis 229 (3), 876-887 (2024)
   PUBMED   37671668
  REMARK    GeneRIF: Homodimeric Granzyme A Opsonizes Mycobacterium
            tuberculosis and Inhibits Its Intracellular Growth in Human
            Monocytes via Toll-Like Receptor 4 and CD14.
REFERENCE   2  (bases 1 to 896)
  AUTHORS   Oltra,S.S., Colomo,S., Sin,L., Perez-Lopez,M., Lazaro,S.,
            Molina-Crespo,A., Choi,K.H., Ros-Pardo,D., Martinez,L., Morales,S.,
            Gonzalez-Paramos,C., Orantes,A., Soriano,M., Hernandez,A.,
            Lluch,A., Rojo,F., Albanell,J., Gomez-Puertas,P., Ko,J.K.,
            Sarrio,D. and Moreno-Bueno,G.
  TITLE     Distinct GSDMB protein isoforms and protease cleavage processes
            differentially control pyroptotic cell death and mitochondrial
            damage in cancer cells
  JOURNAL   Cell Death Differ 30 (5), 1366-1381 (2023)
   PUBMED   36899106
REFERENCE   3  (bases 1 to 896)
  AUTHORS   Gao,Y., Xu,Q., Li,X., Guo,Y., Zhang,B., Jin,Y., Zhu,C., Shen,Y.,
            Yang,P., Shi,Y., Jin,R., Liu,D., Ouyang,Y., Liu,X., Wang,W.,
            Chen,D. and Yang,T.
  TITLE     Heterogeneity induced GZMA-F2R communication inefficient impairs
            antitumor immunotherapy of PD-1 mAb through JAK2/STAT1 signal
            suppression in hepatocellular carcinoma
  JOURNAL   Cell Death Dis 13 (3), 213 (2022)
   PUBMED   35256589
  REMARK    GeneRIF: Heterogeneity induced GZMA-F2R communication inefficient
            impairs antitumor immunotherapy of PD-1 mAb through JAK2/STAT1
            signal suppression in hepatocellular carcinoma.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 896)
  AUTHORS   Hwang,J.H., Hwang,J.H. and Lee,C.S.
  TITLE     Elevated Extracellular Levels of Granzymes in Patients with Scrub
            Typhus
  JOURNAL   Am J Trop Med Hyg 105 (6), 1680-1683 (2021)
   PUBMED   34634775
  REMARK    GeneRIF: Elevated Extracellular Levels of Granzymes in Patients
            with Scrub Typhus.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 896)
  AUTHORS   Rasi,V., Wood,D.C., Eickhoff,C.S., Xia,M., Pozzi,N., Edwards,R.L.,
            Walch,M., Bovenschen,N. and Hoft,D.F.
  TITLE     Granzyme A Produced by gamma9delta2 T Cells Activates ER Stress
            Responses and ATP Production, and Protects Against Intracellular
            Mycobacterial Replication Independent of Enzymatic Activity
  JOURNAL   Front Immunol 12, 712678 (2021)
   PUBMED   34413857
  REMARK    GeneRIF: Granzyme A Produced by gamma9delta2 T Cells Activates ER
            Stress Responses and ATP Production, and Protects Against
            Intracellular Mycobacterial Replication Independent of Enzymatic
            Activity.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 896)
  AUTHORS   Pasternack,M.S., Bleier,K.J. and McInerney,T.N.
  TITLE     Granzyme A binding to target cell proteins. Granzyme A binds to and
            cleaves nucleolin in vitro
  JOURNAL   J Biol Chem 266 (22), 14703-14708 (1991)
   PUBMED   1860869
REFERENCE   7  (bases 1 to 896)
  AUTHORS   Hameed,A., Lowrey,D.M., Lichtenheld,M. and Podack,E.R.
  TITLE     Characterization of three serine esterases isolated from human IL-2
            activated killer cells
  JOURNAL   J Immunol 141 (9), 3142-3147 (1988)
   PUBMED   3262682
REFERENCE   8  (bases 1 to 896)
  AUTHORS   Poe,M., Bennett,C.D., Biddison,W.E., Blake,J.T., Norton,G.P.,
            Rodkey,J.A., Sigal,N.H., Turner,R.V., Wu,J.K. and Zweerink,H.J.
  TITLE     Human cytotoxic lymphocyte tryptase. Its purification from granules
            and the characterization of inhibitor and substrate specificity
  JOURNAL   J Biol Chem 263 (26), 13215-13222 (1988)
   PUBMED   3047119
REFERENCE   9  (bases 1 to 896)
  AUTHORS   Gershenfeld,H.K., Hershberger,R.J., Shows,T.B. and Weissman,I.L.
  TITLE     Cloning and chromosomal assignment of a human cDNA encoding a T
            cell- and natural killer cell-specific trypsin-like serine protease
  JOURNAL   Proc Natl Acad Sci U S A 85 (4), 1184-1188 (1988)
   PUBMED   3257574
REFERENCE   10 (bases 1 to 896)
  AUTHORS   Murphy,M.E., Moult,J., Bleackley,R.C., Gershenfeld,H.,
            Weissman,I.L. and James,M.N.
  TITLE     Comparative molecular model building of two serine proteinases from
            cytotoxic T lymphocytes
  JOURNAL   Proteins 4 (3), 190-204 (1988)
   PUBMED   3237717
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC091977.3.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_006144.3.
            
            Summary: Cytolytic T lymphocytes (CTL) and natural killer (NK)
            cells share the remarkable ability to recognize, bind, and lyse
            specific target cells. They are thought to protect their host by
            lysing cells bearing on their surface 'nonself' antigens, usually
            peptides or proteins resulting from infection by intracellular
            pathogens. The protein described here is a T cell- and natural
            killer cell-specific serine protease that may function as a common
            component necessary for lysis of target cells by cytotoxic T
            lymphocytes and natural killer cells. [provided by RefSeq, Jul
            2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC015739.1, SRR5189667.281315.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000274306.7/ ENSP00000274306.6
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-107               AC091977.3         53243-53349
            108-252             AC091977.3         56071-56215
            253-394             AC091977.3         58391-58532
            395-664             AC091977.3         58722-58991
            665-896             AC091977.3         60618-60849
FEATURES             Location/Qualifiers
     source          1..896
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q11.2"
     gene            1..896
                     /gene="GZMA"
                     /gene_synonym="CTLA3; HFSP"
                     /note="granzyme A"
                     /db_xref="GeneID:3001"
                     /db_xref="HGNC:HGNC:4708"
                     /db_xref="MIM:140050"
     exon            1..107
                     /gene="GZMA"
                     /gene_synonym="CTLA3; HFSP"
                     /inference="alignment:Splign:2.1.0"
     CDS             38..826
                     /gene="GZMA"
                     /gene_synonym="CTLA3; HFSP"
                     /EC_number="3.4.21.78"
                     /note="CTL tryptase; Granzyme A (Cytotoxic
                     T-lymphocyte-associated serine esterase-3; Hanukah factor
                     serine protease); granzyme A (granzyme 1, cytotoxic
                     T-lymphocyte-associated serine esterase 3); HF; h factor;
                     fragmentin-1; cytotoxic T-lymphocyte proteinase 1"
                     /codon_start=1
                     /product="granzyme A precursor"
                     /protein_id="NP_006135.2"
                     /db_xref="CCDS:CCDS3965.1"
                     /db_xref="GeneID:3001"
                     /db_xref="HGNC:HGNC:4708"
                     /db_xref="MIM:140050"
                     /translation="MRNSYRFLASSLSVVVSLLLIPEDVCEKIIGGNEVTPHSRPYMV
                     LLSLDRKTICAGALIAKDWVLTAAHCNLNKRSQVILGAHSITREEPTKQIMLVKKEFP
                     YPCYDPATREGDLKLLQLMEKAKINKYVTILHLPKKGDDVKPGTMCQVAGWGRTHNSA
                     SWSDTLREVNITIIDRKVCNDRNHYNFNPVIGMNMVCAGSLRGGRDSCNGDSGSPLLC
                     EGVFRGVTSFGLENKCGDPRGPGVYILLSKKHLNWIIMTIKGAV"
     exon            108..252
                     /gene="GZMA"
                     /gene_synonym="CTLA3; HFSP"
                     /inference="alignment:Splign:2.1.0"
     exon            253..394
                     /gene="GZMA"
                     /gene_synonym="CTLA3; HFSP"
                     /inference="alignment:Splign:2.1.0"
     exon            395..664
                     /gene="GZMA"
                     /gene_synonym="CTLA3; HFSP"
                     /inference="alignment:Splign:2.1.0"
     exon            665..896
                     /gene="GZMA"
                     /gene_synonym="CTLA3; HFSP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      872..877
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GZMA"
                     /gene_synonym="CTLA3; HFSP"
                     /note="hexamer: AATAAA"
     polyA_site      896
                     /gene="GZMA"
                     /gene_synonym="CTLA3; HFSP"
                     /note="major polyA site"
ORIGIN      
        1 agattttcag gttgattgat gtgggacagc agccacaatg aggaactcct atagatttct
       61 ggcatcctct ctctcagttg tcgtttctct cctgctaatt cctgaagatg tctgtgaaaa
      121 aattattgga ggaaatgaag taactcctca ttcaagaccc tacatggtcc tacttagtct
      181 tgacagaaaa accatctgtg ctggggcttt gattgcaaaa gactgggtgt tgactgcagc
      241 tcactgtaac ttgaacaaaa ggtcccaggt cattcttggg gctcactcaa taaccaggga
      301 agagccaaca aaacagataa tgcttgttaa gaaagagttt ccctatccat gctatgaccc
      361 agccacacgc gaaggtgacc ttaaactttt acagctgatg gaaaaagcaa aaattaacaa
      421 atatgtgact atccttcatc tacctaaaaa gggggacgat gtgaaaccag gaaccatgtg
      481 ccaagttgca gggtggggca ggactcacaa tagtgcatct tggtccgata ctctgagaga
      541 agtcaatatc accatcatag acagaaaagt ctgcaatgat cgaaatcact ataattttaa
      601 ccctgtgatt ggaatgaata tggtttgtgc tggaagcctc cgaggtggaa gagactcgtg
      661 caatggagat tctggaagcc ctttgttgtg cgagggtgtt ttccgagggg tcacttcctt
      721 tggccttgaa aataaatgcg gagaccctcg tgggcctggt gtctatattc ttctctcaaa
      781 gaaacacctc aactggataa ttatgactat caagggagca gtttaaataa ccgtttcctt
      841 tcatttactg tggcttctta atcttttcac aaataaaatc aatttgcatg actgta
//
