LOCUS       NM_001080393            3276 bp    mRNA    linear   PRI 05-APR-2024
DEFINITION  Homo sapiens glucoside xylosyltransferase 2 (GXYLT2), transcript
            variant 1, mRNA.
ACCESSION   NM_001080393 XM_001127141
VERSION     NM_001080393.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Song,Y.B., Bao,W.G., Liu,D.H., Wei,L.Q., Yang,S.T., Miao,X.J.,
            Lin,C.Y., Li,H.J., Lan,D. and He,H.M.
  TITLE     Pan-cancer analysis of the prognostic significance and oncogenic
            role of GXYLT2
  JOURNAL   Medicine (Baltimore) 102 (46), e35664 (2023)
   PUBMED   37986328
  REMARK    GeneRIF: Pan-cancer analysis of the prognostic significance and
            oncogenic role of GXYLT2.
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Wu,Y. and Liao,Q.
  TITLE     MicroRNA-204-5p Hampers the Malignant Progression of Clear Cell
            Renal Cell Carcinoma through GXYLT2 Downregulation
  JOURNAL   Kidney Blood Press Res 47 (11), 654-663 (2022)
   PUBMED   36063796
  REMARK    GeneRIF: MicroRNA-204-5p Hampers the Malignant Progression of Clear
            Cell Renal Cell Carcinoma through GXYLT2 Downregulation.
REFERENCE   3  (bases 1 to 3276)
  AUTHORS   Zhao,Y., Hu,S., Zhang,J., Cai,Z., Wang,S., Liu,M., Dai,J. and
            Gao,Y.
  TITLE     Glucoside xylosyltransferase 2 as a diagnostic and prognostic
            marker in gastric cancer via comprehensive analysis
  JOURNAL   Bioengineered 12 (1), 5641-5654 (2021)
   PUBMED   34506251
  REMARK    GeneRIF: Glucoside xylosyltransferase 2 as a diagnostic and
            prognostic marker in gastric cancer via comprehensive analysis.
REFERENCE   4  (bases 1 to 3276)
  AUTHORS   Cui,Q., Xing,J., Gu,Y., Nan,X., Ma,W., Chen,Y. and Zhao,H.
  TITLE     GXYLT2 accelerates cell growth and migration by regulating the
            Notch pathway in human cancer cells
  JOURNAL   Exp Cell Res 376 (1), 1-10 (2019)
   PUBMED   30716301
  REMARK    GeneRIF: our findings indicated that GXYLT2 plays an important role
            in cell activities via regulation of the Notch signaling
REFERENCE   5  (bases 1 to 3276)
  AUTHORS   Sethi,M.K., Buettner,F.F., Ashikov,A. and Bakker,H.
  TITLE     In vitro assays of orphan glycosyltransferases and their
            application to identify Notch xylosyltransferases
  JOURNAL   Methods Mol Biol 1022, 307-320 (2013)
   PUBMED   23765671
REFERENCE   6  (bases 1 to 3276)
  AUTHORS   Sethi,M.K., Buettner,F.F., Krylov,V.B., Takeuchi,H.,
            Nifantiev,N.E., Haltiwanger,R.S., Gerardy-Schahn,R. and Bakker,H.
  TITLE     Identification of glycosyltransferase 8 family members as
            xylosyltransferases acting on O-glucosylated notch epidermal growth
            factor repeats
  JOURNAL   J Biol Chem 285 (3), 1582-1586 (2010)
   PUBMED   19940119
  REMARK    GeneRIF: We have identified two enzymes of the human
            glycosyltransferase 8 family, now named GXYLT1 and GXYLT2
            (glucoside xylosyltransferase), as UDP-d-xylose:beta-d-glucoside
            alpha1,3-d-xylosyltransferases adding the first xylose.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC114876.2 and AC098481.2.
            
            On May 17, 2019 this sequence version replaced NM_001080393.1.
            
            Summary: The protein encoded by this gene is a xylosyltransferase
            that elongates O-linked glucose bound to epidermal growth factor
            (EGF) repeats. The encoded protein catalyzes the addition of xylose
            to the O-glucose-modified residues of EGF repeats of Notch
            proteins. [provided by RefSeq, Sep 2016].
            
            Transcript Variant: This variant (1) represents the longer,
            protein-coding transcript.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1163655.295093.1,
                                           DRR138528.1045484.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000389617.9/ ENSP00000374268.4
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-188               AC114876.2         57121-57308         c
            189-463             AC114876.2         56846-57120         c
            464-656             AC114876.2         36795-36987         c
            657-788             AC114876.2         23019-23150         c
            789-1040            AC098481.2         133535-133786
            1041-1164           AC098481.2         135666-135789
            1165-1337           AC098481.2         145984-146156
            1338-1735           AC098481.2         153414-153811
            1736-3276           AC098481.2         153812-155352
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p13"
     gene            1..3276
                     /gene="GXYLT2"
                     /gene_synonym="GLT8D4"
                     /note="glucoside xylosyltransferase 2"
                     /db_xref="GeneID:727936"
                     /db_xref="HGNC:HGNC:33383"
                     /db_xref="MIM:613322"
     exon            1..463
                     /gene="GXYLT2"
                     /gene_synonym="GLT8D4"
                     /inference="alignment:Splign:2.1.0"
     CDS             189..1520
                     /gene="GXYLT2"
                     /gene_synonym="GLT8D4"
                     /EC_number="2.4.2.42"
                     /note="glycosyltransferase 8 domain containing 4;
                     glycosyltransferase 8 domain-containing protein 4"
                     /codon_start=1
                     /product="glucoside xylosyltransferase 2 precursor"
                     /protein_id="NP_001073862.1"
                     /db_xref="CCDS:CCDS46870.1"
                     /db_xref="GeneID:727936"
                     /db_xref="HGNC:HGNC:33383"
                     /db_xref="MIM:613322"
                     /translation="MKLRSKAAALLLLALAALLLALLSLRAGRAEPPALPARPASAPQ
                     RHPAPVPARWPGPGALPGASPGVRRRRPPRPRPRAGRRGAARLEKLARRPGEPRSFQA
                     VLPPELWIHLAVVACGNRLEETLVMLKSAVLFSHRKIQFHIFTEDSLKPEFDKQLRQW
                     PDSYTKKFEHRIYPITFSVGNPQEWKKLFKPCAAQRLFLPVILKDVDSLLYVDTDVLF
                     LRPVDDIWKLLRLFNSTQLAAMAPEHEIPKIGWYSRFARHPFYGSAGVNSGVMLMNLT
                     RIRSTQFKNSMIPTGLAWEDMLYPLYQKYKNAITWGDQDLLNIIFYFNPECLYVFPCQ
                     WNYRPDHCMYGSNCREAEHEGVSVLHGNRGVYHDDKQPTFRALYEAIRDFPFQDNLFQ
                     SMYYPLQLKFLETVHTLCGRIPQVFLKQIEKTMKRAYEKHVIIHVGPNQMH"
     exon            464..656
                     /gene="GXYLT2"
                     /gene_synonym="GLT8D4"
                     /inference="alignment:Splign:2.1.0"
     exon            657..788
                     /gene="GXYLT2"
                     /gene_synonym="GLT8D4"
                     /inference="alignment:Splign:2.1.0"
     exon            789..1040
                     /gene="GXYLT2"
                     /gene_synonym="GLT8D4"
                     /inference="alignment:Splign:2.1.0"
     exon            1041..1164
                     /gene="GXYLT2"
                     /gene_synonym="GLT8D4"
                     /inference="alignment:Splign:2.1.0"
     exon            1165..1337
                     /gene="GXYLT2"
                     /gene_synonym="GLT8D4"
                     /inference="alignment:Splign:2.1.0"
     exon            1338..3276
                     /gene="GXYLT2"
                     /gene_synonym="GLT8D4"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      1586
                     /gene="GXYLT2"
                     /gene_synonym="GLT8D4"
                     /note="major polyA site"
     regulatory      3167..3172
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GXYLT2"
                     /gene_synonym="GLT8D4"
                     /note="hexamer: AATAAA"
     regulatory      3180..3185
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GXYLT2"
                     /gene_synonym="GLT8D4"
                     /note="hexamer: AATATA"
     polyA_site      3186
                     /gene="GXYLT2"
                     /gene_synonym="GLT8D4"
                     /note="major polyA site"
     polyA_site      3208
                     /gene="GXYLT2"
                     /gene_synonym="GLT8D4"
                     /note="major polyA site"
     regulatory      3259..3264
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GXYLT2"
                     /gene_synonym="GLT8D4"
                     /note="hexamer: ATTAAA"
     polyA_site      3276
                     /gene="GXYLT2"
                     /gene_synonym="GLT8D4"
ORIGIN      
        1 ctctcctctc tctcctcctc cttcgccgtc gccgccgccg ccggccgccc gccggccgcc
       61 acgaccccag tccccggcgg gcgggcggag gaggcgaccg ccgcgcgctg ctgcactcat
      121 cctgccgccg ccgcgatgcg cagaggggcc gagccgcctg ggggccgccg ccgccgccgc
      181 gccgcaccat gaagctccgc agcaaggcgg cggcgctgct cttgctcgcg ctggccgcgc
      241 tgctgctggc gctgctgtcc ctgcgcgctg gccgcgctga gcccccagcg ctgcccgcgc
      301 gccccgcgtc cgccccgcag cgccaccccg cgcctgtccc cgcgcgctgg ccggggccgg
      361 gcgccctccc cggggccagc ccgggagttc ggaggcgccg gcccccgcgt ccgcgccccc
      421 gagcgggccg ccggggcgct gcgagactgg agaagttggc gaggaggcct ggagaaccca
      481 ggagtttcca agctgtgctg ccacccgagc tctggatcca cctggctgtg gtggcctgtg
      541 gcaatcggct ggaggagacg ctggtcatgc tcaaatcagc tgtgcttttt agccacagga
      601 agatccaatt ccacatcttc actgaagact ctctgaagcc cgagtttgat aagcagttac
      661 gccagtggcc tgactcatat acaaagaagt ttgagcacag aatctacccc atcacatttt
      721 ctgttggaaa ccctcaggag tggaagaaat tgttcaaacc ctgtgctgcc cagagactct
      781 ttcttccggt gattttaaag gatgtggact cacttctcta cgtggacacc gatgtcctct
      841 ttctgagacc tgttgatgac atctggaagc ttctgaggct gtttaattcc acccagcttg
      901 cagccatggc ccctgagcac gaaatcccca agattggctg gtacagccgc tttgctaggc
      961 atcctttcta tggctctgca ggagttaatt caggagtcat gttaatgaat ttaactcgga
     1021 taagaagtac ccagttcaag aacagcatga ttccaacagg cctggcttgg gaggacatgt
     1081 tgtaccctct gtaccagaag tacaagaatg ccatcacgtg gggagaccag gatttattaa
     1141 atattatttt ttatttcaac ccagagtgtc tctatgtatt cccctgccag tggaactacc
     1201 gtcccgatca ctgcatgtac ggaagcaact gcagagaggc tgagcatgaa ggtgtgtctg
     1261 ttctgcatgg aaaccgaggc gtctaccatg acgataagca accaacgttc agagcactct
     1321 atgaagcaat acgggatttt ccctttcaag acaatctctt tcaatccatg tattaccccc
     1381 ttcagctgaa gtttttggag actgtgcaca ctttatgtgg acgaatcccg caagtttttc
     1441 tgaagcaaat tgagaaaaca atgaaaaggg cttatgagaa acacgtcatc atccatgttg
     1501 gccccaacca gatgcactga atattttgtc ttgttgcaag tcaattaggt gtcttgtgac
     1561 caaggaaata ctaatctcta agctgcctgg gtctttttgt gtgaatattt aatggtgctc
     1621 catgactgtt gagttttaaa aacctcgtta aattttgcca aatcagttgc ccccaaaagg
     1681 gaatatgctt ttccttattt ttttttctaa aatgctattt atctctaagg aaaaaaaaaa
     1741 aagactatta ctcatttaac attgtttaag caggttgagc tagctgtgaa aatagctttt
     1801 gtgagccttc taattcctaa acgtctgaga ccatttcagt ggcacctgag gtgttatatt
     1861 gatctagcat tcctgagact ctttctatgc aactgactcc cccacacccg tgttataaaa
     1921 gtaatacaca cttttgaaac agagagacac acttgtagag catatgggtc aagggctcaa
     1981 catcaagatt agattagcca taatcctatt gaccagtgga aaagattgtt tccattttaa
     2041 agcagttcct tacaaccttt tttctctact tttgcatttt ccactctaac acacatctaa
     2101 aacaggtaat ctcagtaaaa gctggccttt ttgctgagat ttttatgtgg gagactaaaa
     2161 tattagatga gatgattatg tgtacagaat tttatttaaa atacatctga gagaatcatt
     2221 cagatacttg ggtagtgtta aaggatgaag catgtatctg ggggtatttc ttctttttct
     2281 ttctttcttt tttttttttt tttttttttt gagacggaat ctcactctgt agcccaggct
     2341 agaatgcagt ggcaggatct cggctcactg cagcttccac ctcctgggtt caagtgattc
     2401 tcatgcctca gcctccccag tagctgggat tacaagtgtg tgccaccaca cccggctaat
     2461 ttttgtattt ttagtagaga cacggtttta ccatgttggc caggctggtc ttgatctcca
     2521 gacctcaagt gatctgcctg cctcagcctc ccaaagtgct gggattacaa gtgtgagcca
     2581 ctgcactcag ccaatctggg agtatttcta aagaaaaaaa ttactttgat ttgtggagga
     2641 ctaaatcata atacatttct atgcaacaat agcttctgag aggaaattca ggaatgtttg
     2701 tgtttaccac agacaaaaac atttgatctg ttaatcatca gtagctgtgg tcatctctct
     2761 ttcttaaact cttgggcaat gggtggaaat cagagaccaa cttcaaattt aatgtgtaaa
     2821 aaacaaatag ttcagctgct tctattggta agaaaattca acttcctacc agccttagaa
     2881 aggtctcttt aaacaatttc tttcccccta gggttaccta gctggtccat acaggtttta
     2941 tcacctcact gcaaggagga agttgaatat tctgttcccc acggggatct ggcttaatcc
     3001 ctagttgctc atatagttgg tatcagcata gcaatatttt gcataatgct ttatttttct
     3061 caaatgacta ttgcatagat gatcttgata tttgcaacag cctagtggat ttggtagggt
     3121 cctgaatcat ctctataagg caaacaagga aattgtaaca caaagaaata aacatattga
     3181 atataattgc tattctgtaa gacatacagt ctgtgtaaga tgtatcttat ttacagagac
     3241 atttttgaaa attaaaatat taaatacttt ttgtta
//
