LOCUS       NM_000857               3382 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens guanylate cyclase 1 soluble subunit beta 1 (GUCY1B1),
            transcript variant 2, mRNA.
ACCESSION   NM_000857
VERSION     NM_000857.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   Korkmaz,Y., Puladi,B., Galler,K., Kammerer,P.W., Schroder,A.,
            Golz,L., Sparwasser,T., Bloch,W., Friebe,A. and Deschner,J.
  TITLE     Inflammation in the Human Periodontium Induces Downregulation of
            the alpha1- and beta1-Subunits of the sGC in Cementoclasts
  JOURNAL   Int J Mol Sci 22 (2), 539 (2021)
   PUBMED   33430449
  REMARK    GeneRIF: Inflammation in the Human Periodontium Induces
            Downregulation of the alpha1- and beta1-Subunits of the sGC in
            Cementoclasts.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3382)
  AUTHORS   Childers,K.C., Yao,X.Q., Giannakoulias,S., Amason,J., Hamelberg,D.
            and Garcin,E.D.
  TITLE     Synergistic mutations in soluble guanylyl cyclase (sGC) reveal a
            key role for interfacial regions in the sGC activation mechanism
  JOURNAL   J Biol Chem 294 (48), 18451-18464 (2019)
   PUBMED   31645439
  REMARK    GeneRIF: Results suggest mechanistic insights into the molecular
            pathway for soluble guanylyl cyclase (sGC) activation.
REFERENCE   4  (bases 1 to 3382)
  AUTHORS   Kang,Y., Liu,R., Wu,J.X. and Chen,L.
  TITLE     Structural insights into the mechanism of human soluble guanylate
            cyclase
  JOURNAL   Nature 574 (7777), 206-210 (2019)
   PUBMED   31514202
REFERENCE   5  (bases 1 to 3382)
  AUTHORS   Rain,M., Chaudhary,H., Kukreti,R., Thinlas,T., Mohammad,G. and
            Pasha,Q.
  TITLE     Elevated Vasodilatory Cyclases and Shorter Telomere Length
            Contribute to High-Altitude Pulmonary Edema
  JOURNAL   High Alt Med Biol 19 (1), 60-68 (2018)
   PUBMED   29443612
  REMARK    GeneRIF: Our observations revealed that rs7638A/C polymorphism of
            GUCY1B3 and the longer telomere length inclined toward adaptation
            to high altitude.
REFERENCE   6  (bases 1 to 3382)
  AUTHORS   Zabel,U., Weeger,M., La,M. and Schmidt,H.H.
  TITLE     Human soluble guanylate cyclase: functional expression and revised
            isoenzyme family
  JOURNAL   Biochem J 335 (Pt 1) (Pt 1), 51-57 (1998)
   PUBMED   9742212
REFERENCE   7  (bases 1 to 3382)
  AUTHORS   Papapetropoulos,A., Cziraki,A., Rubin,J.W., Stone,C.D. and
            Catravas,J.D.
  TITLE     cGMP accumulation and gene expression of soluble guanylate cyclase
            in human vascular tissue
  JOURNAL   J Cell Physiol 167 (2), 213-221 (1996)
   PUBMED   8613461
REFERENCE   8  (bases 1 to 3382)
  AUTHORS   Giuili,G., Roechel,N., Scholl,U., Mattei,M.G. and Guellaen,G.
  TITLE     Colocalization of the genes coding for the alpha 3 and beta 3
            subunits of soluble guanylyl cyclase to human chromosome 4 at
            q31.3-q33
  JOURNAL   Hum Genet 91 (3), 257-260 (1993)
   PUBMED   8097486
REFERENCE   9  (bases 1 to 3382)
  AUTHORS   Giuili,G., Scholl,U., Bulle,F. and Guellaen,G.
  TITLE     Molecular cloning of the cDNAs coding for the two subunits of
            soluble guanylyl cyclase from human brain
  JOURNAL   FEBS Lett 304 (1), 83-88 (1992)
   PUBMED   1352257
REFERENCE   10 (bases 1 to 3382)
  AUTHORS   Chhajlani,V., Frandberg,P.A., Ahlner,J., Axelsson,K.L. and
            Wikberg,J.E.
  TITLE     Heterogeneity in human soluble guanylate cyclase due to alternative
            splicing
  JOURNAL   FEBS Lett 290 (1-2), 157-158 (1991)
   PUBMED   1680753
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DC342619.1, X66533.1, BC026332.1, AI189698.1, DB536632.1 and
            AC114761.3.
            
            On Feb 27, 2020 this sequence version replaced NM_000857.4.
            
            Summary: This gene encodes the beta subunit of the soluble
            guanylate cyclase (sGC), which catalyzes the conversion of GTP
            (guanosine triphosphate) to cGMP (cyclic guanosine monophosphate).
            The encoded protein contains an HNOX domain, which serves as a
            receptor for ligands such as nitric oxide, oxygen and
            nitrovasodilator drugs. Alternative splicing results in multiple
            transcript variants. [provided by RefSeq, May 2014].
            
            Transcript Variant: This variant (2) lacks an alternate in-frame
            exon in the 5' coding region, compared to variant 1. It encodes
            isoform 2, which lacks an internal segment and is shorter than
            isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK315199.1, BC047620.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000264424.13/ ENSP00000264424.8
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-42                DC342619.1         50-91
            43-2475             X66533.1           11-2443
            2476-2785           BC026332.1         2362-2671
            2786-3202           AI189698.1         1-417               c
            3203-3213           DB536632.1         397-407
            3214-3382           AC114761.3         57281-57449
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q32.1"
     gene            1..3382
                     /gene="GUCY1B1"
                     /gene_synonym="GC-S-beta-1; GC-SB3; GUC1B3; GUCB3; GUCSB3;
                     GUCY1B3"
                     /note="guanylate cyclase 1 soluble subunit beta 1"
                     /db_xref="GeneID:2983"
                     /db_xref="HGNC:HGNC:4687"
                     /db_xref="MIM:139397"
     exon            1..123
                     /gene="GUCY1B1"
                     /gene_synonym="GC-S-beta-1; GC-SB3; GUC1B3; GUCB3; GUCSB3;
                     GUCY1B3"
                     /inference="alignment:Splign:2.1.0"
     CDS             121..1980
                     /gene="GUCY1B1"
                     /gene_synonym="GC-S-beta-1; GC-SB3; GUC1B3; GUCB3; GUCSB3;
                     GUCY1B3"
                     /EC_number="4.6.1.2"
                     /note="isoform 2 is encoded by transcript variant 2;
                     guanylate cyclase soluble subunit beta-1; GCS-beta-1;
                     GCS-beta-3; soluble guanylate cyclase small subunit;
                     guanylate cyclase soluble subunit beta-3; guanylate
                     cyclase 1, soluble, beta 3"
                     /codon_start=1
                     /product="guanylate cyclase soluble subunit beta-1 isoform
                     2"
                     /protein_id="NP_000848.1"
                     /db_xref="CCDS:CCDS47154.1"
                     /db_xref="GeneID:2983"
                     /db_xref="HGNC:HGNC:4687"
                     /db_xref="MIM:139397"
                     /translation="MYGFVNHALELLVIRNYGPEVWEDIKKEAQLDEEGQFLVRIIYD
                     DSKTYDLVAAASKVLNLNAGEILQMFGKMFFVFCQESGYDTILRVLGSNVREFLQNLD
                     ALHDHLATIYPGMRAPSFRCTDAEKGKGLILHYYSEREGLQDIVIGIIKTVAQQIHGT
                     EIDMKVIQQRNEECDHTQFLIEEKESKEEDFYEDLDRFEENGTQESRISPYTFCKAFP
                     FHIIFDRDLVVTQCGNAIYRVLPQLQPGNCSLLSVFSLVRPHIDISFHGILSHINTVF
                     VLRSKEGLLDVEKLECEDELTGTEISCLRLKGQMIYLPEADSILFLCSPSVMNLDDLT
                     RRGLYLSDIPLHDATRDLVLLGEQFREEYKLTQELEILTDRLQLTLRALEDEKKKTDT
                     LLYSVLPPSVANELRHKRPVPAKRYDNVTILFSGIVGFNAFCSKHASGEGAMKIVNLL
                     NDLYTRFDTLTDSRKNPFVYKVETVGDKYMTVSGLPEPCIHHARSICHLALDMMEIAG
                     QVQVDGESVQITIGIHTGEVVTGVIGQRMPRYCLFGNTVNLTSRTETTGEKGKINVSE
                     YTYRCLMSPENSDPQFHLEHRGPVSMKGKKEPMQVWFLSRKNTGTEETKQDDD"
     exon            124..197
                     /gene="GUCY1B1"
                     /gene_synonym="GC-S-beta-1; GC-SB3; GUC1B3; GUCB3; GUCSB3;
                     GUCY1B3"
                     /inference="alignment:Splign:2.1.0"
     exon            198..298
                     /gene="GUCY1B1"
                     /gene_synonym="GC-S-beta-1; GC-SB3; GUC1B3; GUCB3; GUCSB3;
                     GUCY1B3"
                     /inference="alignment:Splign:2.1.0"
     exon            299..417
                     /gene="GUCY1B1"
                     /gene_synonym="GC-S-beta-1; GC-SB3; GUC1B3; GUCB3; GUCSB3;
                     GUCY1B3"
                     /inference="alignment:Splign:2.1.0"
     exon            418..615
                     /gene="GUCY1B1"
                     /gene_synonym="GC-S-beta-1; GC-SB3; GUC1B3; GUCB3; GUCSB3;
                     GUCY1B3"
                     /inference="alignment:Splign:2.1.0"
     exon            616..846
                     /gene="GUCY1B1"
                     /gene_synonym="GC-S-beta-1; GC-SB3; GUC1B3; GUCB3; GUCSB3;
                     GUCY1B3"
                     /inference="alignment:Splign:2.1.0"
     exon            847..963
                     /gene="GUCY1B1"
                     /gene_synonym="GC-S-beta-1; GC-SB3; GUC1B3; GUCB3; GUCSB3;
                     GUCY1B3"
                     /inference="alignment:Splign:2.1.0"
     exon            964..1097
                     /gene="GUCY1B1"
                     /gene_synonym="GC-S-beta-1; GC-SB3; GUC1B3; GUCB3; GUCSB3;
                     GUCY1B3"
                     /inference="alignment:Splign:2.1.0"
     exon            1098..1295
                     /gene="GUCY1B1"
                     /gene_synonym="GC-S-beta-1; GC-SB3; GUC1B3; GUCB3; GUCSB3;
                     GUCY1B3"
                     /inference="alignment:Splign:2.1.0"
     exon            1296..1533
                     /gene="GUCY1B1"
                     /gene_synonym="GC-S-beta-1; GC-SB3; GUC1B3; GUCB3; GUCSB3;
                     GUCY1B3"
                     /inference="alignment:Splign:2.1.0"
     exon            1534..1674
                     /gene="GUCY1B1"
                     /gene_synonym="GC-S-beta-1; GC-SB3; GUC1B3; GUCB3; GUCSB3;
                     GUCY1B3"
                     /inference="alignment:Splign:2.1.0"
     exon            1675..1829
                     /gene="GUCY1B1"
                     /gene_synonym="GC-S-beta-1; GC-SB3; GUC1B3; GUCB3; GUCSB3;
                     GUCY1B3"
                     /inference="alignment:Splign:2.1.0"
     exon            1830..1956
                     /gene="GUCY1B1"
                     /gene_synonym="GC-S-beta-1; GC-SB3; GUC1B3; GUCB3; GUCSB3;
                     GUCY1B3"
                     /inference="alignment:Splign:2.1.0"
     exon            1957..3382
                     /gene="GUCY1B1"
                     /gene_synonym="GC-S-beta-1; GC-SB3; GUC1B3; GUCB3; GUCSB3;
                     GUCY1B3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3141..3146
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GUCY1B1"
                     /gene_synonym="GC-S-beta-1; GC-SB3; GUC1B3; GUCB3; GUCSB3;
                     GUCY1B3"
                     /note="hexamer: AATAAA"
     polyA_site      3162
                     /gene="GUCY1B1"
                     /gene_synonym="GC-S-beta-1; GC-SB3; GUC1B3; GUCB3; GUCSB3;
                     GUCY1B3"
                     /note="major polyA site"
     regulatory      3184..3189
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GUCY1B1"
                     /gene_synonym="GC-S-beta-1; GC-SB3; GUC1B3; GUCB3; GUCSB3;
                     GUCY1B3"
                     /note="hexamer: AATAAA"
     polyA_site      3202
                     /gene="GUCY1B1"
                     /gene_synonym="GC-S-beta-1; GC-SB3; GUC1B3; GUCB3; GUCSB3;
                     GUCY1B3"
     regulatory      3347..3352
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GUCY1B1"
                     /gene_synonym="GC-S-beta-1; GC-SB3; GUC1B3; GUCB3; GUCSB3;
                     GUCY1B3"
                     /note="hexamer: AATAAA"
     polyA_site      3382
                     /gene="GUCY1B1"
                     /gene_synonym="GC-S-beta-1; GC-SB3; GUC1B3; GUCB3; GUCSB3;
                     GUCY1B3"
ORIGIN      
        1 gctccagctc gatgctgcct ccccggcccg gttgcgctgt agccgctgcc gcctctgcct
       61 gggtcccttc ggccgtacct ctgcgtgggg gctgcctccc cggctcccgg tgcagacacc
      121 atgtacggat ttgtgaatca cgccctggag ttgctggtga tccgcaatta cggccccgag
      181 gtgtgggaag acatcaaaaa agaggcacag ttagatgaag aaggacagtt tcttgtcaga
      241 ataatatatg atgactccaa aacttatgat ttggttgctg ctgcaagcaa agtcctcaat
      301 ctcaatgctg gagaaatcct ccaaatgttt gggaagatgt ttttcgtctt ttgccaagaa
      361 tctggttatg atacaatctt gcgtgtcctg ggctctaatg tcagagaatt tctacagaac
      421 cttgatgctc tgcacgacca ccttgctacc atctacccag gaatgcgtgc accttccttt
      481 aggtgcactg atgcagaaaa gggcaaagga ctcattttgc actactactc agagagagaa
      541 ggacttcagg atattgtcat tggaatcatc aaaacagtgg cacaacaaat ccatggcact
      601 gaaatagaca tgaaggttat tcagcaaaga aatgaagaat gtgatcatac tcaattttta
      661 attgaagaaa aagagtcaaa agaagaggat ttttatgaag atcttgacag atttgaagaa
      721 aatggtaccc aggaatcacg catcagccca tatacattct gcaaagcttt tccttttcat
      781 ataatatttg accgggacct agtggtcact cagtgtggca atgctatata cagagttctc
      841 ccccagctcc agcctgggaa ttgcagcctt ctgtctgtct tctcgctggt tcgtcctcat
      901 attgatatta gtttccatgg gatcctttct cacatcaata ctgtttttgt attgagaagc
      961 aaggaaggat tgttggatgt ggagaaatta gaatgtgagg atgaactgac tgggactgag
     1021 atcagctgct tacgtctcaa gggtcaaatg atctacttac ctgaagcaga tagcatactt
     1081 tttctatgtt caccaagtgt catgaacctg gacgatttga caaggagagg gctgtatcta
     1141 agtgacatcc ctctgcatga tgccacgcgc gatcttgttc ttttgggaga acaatttaga
     1201 gaggaataca aactcaccca agaactggaa atcctcactg acaggctaca gctcacgtta
     1261 agagccctgg aagatgaaaa gaaaaagaca gacacattgc tgtattctgt ccttcctccg
     1321 tctgttgcca atgagctgcg gcacaagcgt ccagtgcctg ccaaaagata tgacaatgtg
     1381 accatcctct ttagtggcat tgtgggcttc aatgctttct gtagcaagca tgcatctgga
     1441 gaaggagcca tgaagatcgt caacctcctc aacgacctct acaccagatt tgacacactg
     1501 actgattccc ggaaaaaccc atttgtttat aaggtggaga ctgttggtga caagtatatg
     1561 acagtgagtg gtttaccaga gccatgcatt caccatgcac gatccatctg ccacctggcc
     1621 ttggacatga tggaaattgc tggccaggtt caagtagatg gtgaatctgt tcagataaca
     1681 atagggatac acactggaga ggtagttaca ggtgtcatag gacagcggat gcctcgatac
     1741 tgtctttttg ggaatactgt caacctcaca agccgaacag aaaccacagg agaaaaggga
     1801 aaaataaatg tgtctgaata tacatacaga tgtcttatgt ctccagaaaa ttcagatcca
     1861 caattccact tggagcacag aggcccagtg tccatgaagg gcaaaaaaga accaatgcaa
     1921 gtttggtttc tatccagaaa aaatacagga acagaggaaa caaagcagga tgatgactga
     1981 atcttggatt atggggtgaa gaggagtaca gactaggttc cagttttctc ctaacacgtg
     2041 ccaagcccag gagcagttct tccctatgga tacagatttt cttttgtcct tgtccattac
     2101 cccaagactt tcttctagat atatctctca ctatccgtta ttcaacctta gctctgcttt
     2161 ctattacttt ttaggcttta gtatattatc taaagtttgg cttttgatgt ggatgatgtg
     2221 agcttcatgt gtcttaaaat ctactacaag cattacctaa catggtgatc tgcaagtagt
     2281 aggcacccaa taaatatttg ttgaatttag ttaaatgaaa ctgaacagtg tttggccatg
     2341 tgtatattta tatcatgttt accaaatctg tttagtgttc cacatatatg tatatgtata
     2401 ttttaatgac tataatgtaa taaagtttat atcatgttgg tgtatatcat tatagaaatc
     2461 attttctaaa ggagtgaatt ctaagtttta ggggaaaaaa tgcaatttat tttcagactc
     2521 ccaaagtaag aattaacata tcatgctaag aaaatagtga ctattttgaa gtatgctact
     2581 tccctttcag aaatatagaa tacacgtttc tgttattaaa gtatttgatt actaattcaa
     2641 atcatatggc aattataatt cttctaaaat gctatcattt gtaactgtat cccctgtatt
     2701 aaatctcatt aaccacaggc agctgttaca gaaagctgca ttgtttcaca ttgagctgtt
     2761 acattagttc aggctaaatg ttgggagctc caaccacatc caagaataaa tctggaaaca
     2821 cactgctggg atactgctgt tagagccctt cttggccttg tattcccaga aatgagctcc
     2881 ctttccttag cttagaagaa tgtgattata tccaggacat catgttcaga aaacttagtt
     2941 tactttcagc atagaatgca ttactgttgg aataattggc ctctagctct taaatgtctc
     3001 tgataactta ttaatatcta tctttataaa atagagtgca actacttttg tgtaaaaatg
     3061 tttgccttta aatttagtat ttcatatcag cacatcgata tatgtataaa tgttccatgt
     3121 taatgtgtaa aagagtctgt aataaattat ttttttcacg tgtctctata cagtttttat
     3181 ttcaataaaa atattaacat tatttttcat tttattaatg ccattatttt gtaaatacat
     3241 tttacaattt tgcctatttg ctaccattat aaattttcac tgactcctca tagacagcat
     3301 attcttaaat gctatatttc tttttaatac caacagagtg acaggaaata aagactgggc
     3361 atggaaaggg gaaacagtta aa
//
