LOCUS       NM_000855              16150 bp    mRNA    linear   PRI 06-APR-2024
DEFINITION  Homo sapiens guanylate cyclase 1 soluble subunit alpha 2 (GUCY1A2),
            transcript variant 2, mRNA.
ACCESSION   NM_000855
VERSION     NM_000855.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 16150)
  AUTHORS   Zhao,D., Dong,Y., Duan,M., He,D., Xie,Q., Peng,W., Cui,W.,
            Jiang,J., Cheng,Y., Zhang,H., Tang,F., Zhang,C., Gao,Y. and Duan,C.
  TITLE     Circadian gene ARNTL initiates circGUCY1A2 transcription to
            suppress non-small cell lung cancer progression via
            miR-200c-3p/PTEN signaling
  JOURNAL   J Exp Clin Cancer Res 42 (1), 229 (2023)
   PUBMED   37667322
  REMARK    GeneRIF: Circadian gene ARNTL initiates circGUCY1A2 transcription
            to suppress non-small cell lung cancer progression via
            miR-200c-3p/PTEN signaling.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16150)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 16150)
  AUTHORS   Sundell,G.N., Arnold,R., Ali,M., Naksukpaiboon,P., Orts,J.,
            Guntert,P., Chi,C.N. and Ivarsson,Y.
  TITLE     Proteome-wide analysis of phospho-regulated PDZ domain interactions
  JOURNAL   Mol Syst Biol 14 (8), e8129 (2018)
   PUBMED   30126976
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 16150)
  AUTHORS   Otowa,T., Maher,B.S., Aggen,S.H., McClay,J.L., van den Oord,E.J.
            and Hettema,J.M.
  TITLE     Genome-wide and gene-based association studies of anxiety disorders
            in European and African American samples
  JOURNAL   PLoS One 9 (11), e112559 (2014)
   PUBMED   25390645
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 16150)
  AUTHORS   Seeger,F., Quintyn,R., Tanimoto,A., Williams,G.J., Tainer,J.A.,
            Wysocki,V.H. and Garcin,E.D.
  TITLE     Interfacial residues promote an optimal alignment of the catalytic
            center in human soluble guanylate cyclase: heterodimerization is
            required but not sufficient for activity
  JOURNAL   Biochemistry 53 (13), 2153-2165 (2014)
   PUBMED   24669844
  REMARK    GeneRIF: The data support a novel regulatory mechanism whereby sGC
            activity is tuned by distinct domain interactions that either
            promote or inhibit catalytic activity.
REFERENCE   6  (bases 1 to 16150)
  AUTHORS   Bamberger,A.M., Koglin,M., Kempfert,J., Loning,T., Scholz,H. and
            Behrends,S.
  TITLE     Expression and tissue localization of soluble guanylyl cyclase in
            the human placenta using novel antibodies directed against the
            alpha(2) subunit
  JOURNAL   J Clin Endocrinol Metab 86 (2), 909-912 (2001)
   PUBMED   11158065
REFERENCE   7  (bases 1 to 16150)
  AUTHORS   Budworth,J., Meillerais,S., Charles,I. and Powell,K.
  TITLE     Tissue distribution of the human soluble guanylate cyclases
  JOURNAL   Biochem Biophys Res Commun 263 (3), 696-701 (1999)
   PUBMED   10512742
REFERENCE   8  (bases 1 to 16150)
  AUTHORS   Yu,F., Warburton,D., Wellington,S. and Danziger,R.S.
  TITLE     Assignment of GUCIA2, the gene coding for the alpha 2 subunit of
            soluble guanylyl cyclase, to position 11q21-q22 on human chromosome
            11
  JOURNAL   Genomics 33 (2), 334-336 (1996)
   PUBMED   8660992
REFERENCE   9  (bases 1 to 16150)
  AUTHORS   Behrends,S., Harteneck,C., Schultz,G. and Koesling,D.
  TITLE     A variant of the alpha 2 subunit of soluble guanylyl cyclase
            contains an insert homologous to a region within adenylyl cyclases
            and functions as a dominant negative protein
  JOURNAL   J Biol Chem 270 (36), 21109-21113 (1995)
   PUBMED   7673142
REFERENCE   10 (bases 1 to 16150)
  AUTHORS   Harteneck,C., Wedel,B., Koesling,D., Malkewitz,J., Bohme,E. and
            Schultz,G.
  TITLE     Molecular cloning and expression of a new alpha-subunit of soluble
            guanylyl cyclase. Interchangeability of the alpha-subunits of the
            enzyme
  JOURNAL   FEBS Lett 292 (1-2), 217-222 (1991)
   PUBMED   1683630
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AP003078.2, X63282.1,
            AP005014.3 and CA774108.1.
            
            On Jun 2, 2019 this sequence version replaced NM_000855.2.
            
            Summary: Soluble guanylate cyclases are heterodimeric proteins that
            catalyze the conversion of GTP to 3',5'-cyclic GMP and
            pyrophosphate. The protein encoded by this gene is an alpha subunit
            of this complex and it interacts with a beta subunit to form the
            guanylate cyclase enzyme, which is activated by nitric oxide. Two
            transcript variants encoding different isoforms have been found for
            this gene. [provided by RefSeq, Jan 2012].
            
            Transcript Variant: This variant (2) lacks an alternate in-frame
            exon compared to variant 1. The resulting isoform (2) has the same
            N- and C-termini but is shorter compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X63282.1, BC130488.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000526355.7/ ENSP00000431245.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-31                AP003078.2         39798-39828         c
            32-2985             X63282.1           1-2954
            2986-16136          AP005014.3         90882-104032        c
            16137-16150         CA774108.1         22-35               c
FEATURES             Location/Qualifiers
     source          1..16150
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q22.3"
     gene            1..16150
                     /gene="GUCY1A2"
                     /gene_synonym="GC-SA2; GUC1A2"
                     /note="guanylate cyclase 1 soluble subunit alpha 2"
                     /db_xref="GeneID:2977"
                     /db_xref="HGNC:HGNC:4684"
                     /db_xref="MIM:601244"
     exon            1..724
                     /gene="GUCY1A2"
                     /gene_synonym="GC-SA2; GUC1A2"
                     /inference="alignment:Splign:2.1.0"
     CDS             422..2620
                     /gene="GUCY1A2"
                     /gene_synonym="GC-SA2; GUC1A2"
                     /EC_number="4.6.1.2"
                     /note="isoform 2 is encoded by transcript variant 2;
                     guanylate cyclase soluble subunit alpha-2; GCS-alpha-2;
                     guanylate cyclase 1, soluble, alpha 2"
                     /codon_start=1
                     /product="guanylate cyclase soluble subunit alpha-2
                     isoform 2"
                     /protein_id="NP_000846.1"
                     /db_xref="CCDS:CCDS8335.1"
                     /db_xref="GeneID:2977"
                     /db_xref="HGNC:HGNC:4684"
                     /db_xref="MIM:601244"
                     /translation="MSRRKISSESFSSLGSDYLETSPEEEGECPLSRLCWNGSRSPPG
                     PLEPSPAAAAAAAAPAPTPAASAAAAAATAGARRVQRRRRVNLDSLGESISRLTAPSP
                     QTIQQTLKRTLQYYEHQVIGYRDAEKNFHNISNRCSYADHSNKEEIEDVSGILQCTAN
                     ILGLKFEEIQKRFGEEFFNICFHENERVLRAVGGTLQDFFNGFDALLEHIRTSFGKQA
                     TLESPSFLCKELPEGTLMLHYFHPHHIVGFAMLGMIKAAGKKIYRLDVEVEQVANEKL
                     CSDVSNPGNCSCLTFLIKECENTNIMKNLPQGTSQVPADLRISINTFCRAFPFHLMFD
                     PSMSVLQLGEGLRKQLRCDTHKVLKFEDCFEIVSPKVNATFERVLLRLSTPFVIRTKP
                     EASGSENKDKVMEVKGQMIHVPESNSILFLGSPCVDKLDELMGRGLHLSDIPIHDATR
                     DVILVGEQAKAQDGLKKRMDKLKATLERTHQALEEEKKKTVDLLYSIFPGDVAQQLWQ
                     GQQVQARKFDDVTMLFSDIVGFTAICAQCTPMQVISMLNELYTRFDHQCGFLDIYKVE
                     TIGDAYCVAAGLHRKSLCHAKPIALMALKMMELSEEVLTPDGRPIQMRIGIHSGSVLA
                     GVVGVRMPRYCLFGNNVTLASKFESGSHPRRINVSPTTYQLLKREESFTFIPRSREEL
                     PDNFPKEIPGICYFLEVRTGPKPPKPSLSSSRIKKVSYNIGTMFLRETSL"
     exon            725..786
                     /gene="GUCY1A2"
                     /gene_synonym="GC-SA2; GUC1A2"
                     /inference="alignment:Splign:2.1.0"
     exon            787..908
                     /gene="GUCY1A2"
                     /gene_synonym="GC-SA2; GUC1A2"
                     /inference="alignment:Splign:2.1.0"
     exon            909..1627
                     /gene="GUCY1A2"
                     /gene_synonym="GC-SA2; GUC1A2"
                     /inference="alignment:Splign:2.1.0"
     exon            1628..2113
                     /gene="GUCY1A2"
                     /gene_synonym="GC-SA2; GUC1A2"
                     /inference="alignment:Splign:2.1.0"
     exon            2114..2257
                     /gene="GUCY1A2"
                     /gene_synonym="GC-SA2; GUC1A2"
                     /inference="alignment:Splign:2.1.0"
     exon            2258..2412
                     /gene="GUCY1A2"
                     /gene_synonym="GC-SA2; GUC1A2"
                     /inference="alignment:Splign:2.1.0"
     exon            2413..16150
                     /gene="GUCY1A2"
                     /gene_synonym="GC-SA2; GUC1A2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2792..2797
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GUCY1A2"
                     /gene_synonym="GC-SA2; GUC1A2"
                     /note="hexamer: AATAAA"
     polyA_site      2817
                     /gene="GUCY1A2"
                     /gene_synonym="GC-SA2; GUC1A2"
     regulatory      16130..16135
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GUCY1A2"
                     /gene_synonym="GC-SA2; GUC1A2"
                     /note="hexamer: AATAAA"
     polyA_site      16150
                     /gene="GUCY1A2"
                     /gene_synonym="GC-SA2; GUC1A2"
                     /note="major polyA site"
ORIGIN      
        1 agaagcctct gcctctgcct ccccttctgc ttgggccgca gccctccccg ccccgccgac
       61 cgcggtcaca cactctcgga gcctccccgt gagcgggagc gcggcgcacg gcgatgcgcc
      121 gaggcgggcg ctgaggcggc gccgcgcgag cagcagcaga ggcggcggcg gcccccagcc
      181 cagcccggcg ccgccgccga gcccgggccc caaggtgcgg cggcgcccca agttcccgcc
      241 atgagcagcc ggctcggggg gctccgcggc cccggggact cccgccccgc cgggcgcgac
      301 cgcagcgccc cgcggccccg acgcgcttaa cgttgtcgct tgccggtccc gccaccgccg
      361 cctccgccgc cgctcgcgtc ctcgccgcca ccgcctcggc cgctgcagct ccgccggcag
      421 catgtctcga aggaagattt cgtccgagtc cttcagctcc ctgggctccg actacctgga
      481 gaccagcccg gaggaggagg gggagtgccc cctgtctagg ctctgctgga atggcagccg
      541 gagcccgccc gggccgctgg agcccagccc ggccgcagct gccgctgccg ccgccccggc
      601 cccgaccccg gctgcttctg ccgccgccgc cgctgccact gccggggcca ggagggtgca
      661 gcgccggagg cgggtcaacc tggactcgct gggcgagagc atcagccgcc tgacggcgcc
      721 ctcgcctcag acgatacagc agactctcaa gaggacactg cagtattatg aacatcaagt
      781 tattggttac agggatgcag aaaagaattt ccacaatatc tctaacagat gctcctatgc
      841 agaccactcc aacaaagaag aaattgaaga tgtctcagga attcttcagt gtactgctaa
      901 tatactcggt ttgaagtttg aggaaattca aaaaagattt ggtgaagagt tctttaatat
      961 atgctttcat gagaatgaga gagtccttcg agctgtaggt ggcactttgc aggacttttt
     1021 taacggcttt gatgctttgt tggaacacat tagaacttct tttggaaaac aggccactct
     1081 ggagtcacca tctttcctat gcaaagagct ccctgaaggt actctcatgc tccactactt
     1141 ccaccctcac catattgtgg ggtttgcaat gctggggatg attaaggctg caggaaagaa
     1201 gatctatcgg ctggatgtgg aagtggaaca ggttgcaaat gagaagctat gctctgatgt
     1261 ttcaaaccca ggcaattgta gctgtcttac tttccttatc aaagaatgtg aaaatactaa
     1321 tatcatgaag aaccttccac agggaacctc ccaagttcct gcggacctca gaattagcat
     1381 caacaccttc tgtagagcct tccctttcca cttgatgttt gatcccagca tgtcagtcct
     1441 tcagttgggg gaaggtctaa ggaagcagct tcgatgtgac actcacaaag tgctcaagtt
     1501 tgaggactgc ttcgagattg tatctccaaa ggttaatgcc acctttgaaa gggtcctgct
     1561 gcgactgtct accccgtttg tgattagaac caagcctgag gcttctggct ctgaaaataa
     1621 agacaaggtg atggaagtca aaggacaaat gatccatgtt ccagaatcaa attccatttt
     1681 atttttgggc tctccatgtg tggacaagtt ggatgaactc atgggccgag ggctacatct
     1741 ctcagacatc cctatccatg atgccacccg agatgtcatt ttggttggtg agcaggcaaa
     1801 ggcccaagat gggttgaaga aaaggatgga taaattaaag gcaactttag aaagaactca
     1861 ccaggccctg gaagaagaga aaaagaagac agtggatctt ctatattcta ttttccctgg
     1921 tgatgtagcc cagcaattat ggcaagggca gcaagtacag gccagaaagt ttgatgatgt
     1981 caccatgctc ttttcagaca ttgttggctt cacagccata tgtgcccagt gtactcccat
     2041 gcaagtaatc agcatgctga atgaactgta caccagattt gaccaccagt gtggattttt
     2101 ggatatttat aaggtggaaa caataggtga tgcctactgt gttgcagcag ggctccacag
     2161 aaaaagcctc tgccatgcta aacccattgc tctgatggcc ttgaagatga tggaactttc
     2221 agaagaggtg ctgacacctg atggaagacc gattcagatg aggataggaa ttcactcagg
     2281 ctccgtgctg gctggagttg ttggggtgcg aatgccacgt tattgcctgt ttggaaataa
     2341 tgtcacactg gcaagcaaat tcgagtcggg aagtcaccct cggcgcatca atgtcagccc
     2401 aaccacttac caattattaa aacgagaaga aagtttcaca ttcattccgc ggtctcgtga
     2461 agagcttcca gacaactttc caaaggaaat tcctgggatc tgctatttcc tggaggtaag
     2521 gactggtcca aagccaccaa agccttctct ttcttcgtcg agaataaaaa aggtttccta
     2581 caacatcggc accatgttcc tccgggagac aagcctctga gacctgctac agatcaaaga
     2641 ctcctccaaa aagcacaagc ccagaacatg ggtcaccaat ggggggtgga aagagattgt
     2701 gtctctttca ttgctttgtt gagaacaagc agcaaaattt ctgtattatg tcaggcaata
     2761 atcctactaa aaggtggagg tgaccgctgt caataaaaag ccggaggatg agggaaataa
     2821 gatgtgtcca ttcatatgag tggttttggt catatatata cacatatatt ttaattacaa
     2881 gtgtgggtcc cctttcagaa ctaaccaata aatagattcc atgttttctt gtttatcaca
     2941 catacaagta tctttcccta tatatttgta ccacttttga gagccagttt tgattatata
     3001 ttcctacatt tagtgagttg gtgggtgagg aatattttct aagtttcttt ttctgaacag
     3061 acatttcata catgtatcat ggcagtgtgg taaacttccc aactggaaga attgtaactc
     3121 ggcaaaatat tttagggata ttacctattt ttatacatat ctcttctaga taactacatt
     3181 tcgcacaaca ttattagatt ttcagaatct gcttctgtat tgttagtata ttatatataa
     3241 tgctaaccta ttaatctaat atagtttccc ttatttagag agatcagtgc ttcgactgag
     3301 aatacatata accaaggatt tcagatacca agaaagcatt atatgtctgc ttagtaaata
     3361 tatcttttag atttaactaa tgtagcatgg aatccagagg aaatggaatt tcctaacacc
     3421 agatgtatct gtaaccaaga ttaaagaaag gtctttttgc caccttctaa caaatatcag
     3481 ttaaaatgaa atgctttgct acactggatc cttttcaact tagcacttac aaatataggt
     3541 agcttttgca acactaaact gcattctaat aaacagcatt cagctaaatg acatgttagt
     3601 tacccccttc ctcttcccca gttgatgcct cctaagagcc gactgctatt cttgagaaca
     3661 aattcctgga tactggcatt agtgacaaag aagtgtcagg acagtttgcc aagaatgttg
     3721 tttcttcttg tacaacacaa agcaaggaaa cctctgtatt ttatggcttt gaggttttaa
     3781 aaaatgttct gcagtttgat tgatatggct tttaaattca gtgaatcact ttcatgaata
     3841 ctgaagtagg tataatcttc agaactagta atcattatag ttacagatag gattccatcg
     3901 catgtcctgc tcaaatgaag acctgttctc attaatcaat actgcatgaa aatattactg
     3961 gctcacacta aaattaggag catcaggtgt ccagagtttt cctgtttggg ttcagatata
     4021 aaatctaagt ttctattgag gttaataagc tggaagcatt tgtttccttt aagccttgga
     4081 gaggtcattt tcttactcat tgatagctta tgtctactga atgcaatatt cttttctgtt
     4141 cagggatttt ggctgcatta tgatttggtt atgacataac tcagttaagg aatatattta
     4201 ctaatttcca atgtagtcta tgaggttcat ttaatgtttt taaattttct atgtgtgtgg
     4261 ctagaaggca tttgtagata tgcaaatatt tgttataatc ctgatccctt ttataatttg
     4321 agttattttc ttgccctggg ggtttacaag gacacgagtg caacactctt tatatggaga
     4381 agacaggaaa tagaccttga gctctaaacc tttgcctgac cccctcttct gtgggcctgt
     4441 gtttctggac aatggggagg tttacagcca gtatgagcag agcagactgg gagcacctca
     4501 atcctcatgc ttatattaga aaatgcctta gcaatgttga tgggctcctt gtagcactgg
     4561 agcatgggga gggaacaagc tcactgatgt catttatgcc tattgtcttc agtaacttag
     4621 aacttattat ggtatattaa atcaataaaa tgttggtgat gaacaatgtg ttttgtaacc
     4681 ataaggctga aagggacttc caggaatatc caatctattt ctctcacttt ttataaagat
     4741 ttgtctaagc aactactcta aacacatatt tattatctcc atacttaaga atatctacgg
     4801 gaagcatatt tgaaaagcat cattggaaat ccacctcaat actagtgata ggcctggaga
     4861 gaagtattgt atttatatat ctcaatttat cagaagaatc acaaattctg tattagttta
     4921 tatgatttta cttagagaat aagtatccat ttacattgcc ataaatgtca cgatgaagaa
     4981 cgtgccagag atttcttatt tatcactgac aggatgtcaa ctacattttt catacattat
     5041 aacctaagtg tattttcaca gctatagtcc gagataatgg tccaatttgt tatcattttg
     5101 ctaatctatt atagttgatt attgtagtta caatgttgct atatatgttt atgaggtact
     5161 atataaaatg tctgttcatt caaacttcaa tcacagaagt tagaagatgg agaaaaaatt
     5221 atatttttat ttgtcctaca caatgccatc tacatctctt ctcttaggaa ttgtgattgt
     5281 aacaatgtgg tggcatttgt tatttcatat gacttttatc tatacagtat tctcacttac
     5341 ttacaaacta ttaccaacag catgcatttt agtatggcag atacaagaaa tttgtgtgat
     5401 gttcccttag aaaccaaatg atattaaatt ggggaaatgg ttttaaccca ctctgcaagg
     5461 gatactttaa aatataattt ggcacctata gctcttgcat tttaatattc ttttaagctg
     5521 ctgtttggaa gaaggcacct tataactagc cattagcacc aaagcatcac gttggtatca
     5581 ggattatttt gatggagaga atgtcatcat ttgcaaataa atcagttatc taacaaaatc
     5641 agaatccccc aatatgtact cagaatgtac taccctgtat cttctttgtg gaccttaatc
     5701 attaggtaac attggccata ataaaaaata ataatatgct tcagaaacat agtagttgga
     5761 tctcttgtct atataacgta gagaagggga agaagacata gcttccaggt aacatgggac
     5821 ccaattgcgt acaatctgcc tttatattca tctcttgctc tatattttaa gtaatttcag
     5881 attttccttt taatatctga ttatgtggaa tcaagtgagc tttgatctgg gactctgaga
     5941 cagacattga tgcctgagac ttgagagtgg gtctgtaggt tcagcacttc aaatgttgtt
     6001 tcttacagtc ttgcactcct tcctcttgtc cagtaacaac ggtgtcagga cacagtagtt
     6061 tagtgtgaat atgtgtgtct ttccaggaaa aacaaagcaa aagaggataa taacaactta
     6121 aacaaaatct cattcacaag gggagcaaga cttttaattt tgcaaatgcc tccaaagagt
     6181 tccagcaata aagcaaaatt aaattgatag ataacaaaag gcaaagccat ggagaattct
     6241 gaaaacccca ccaggctctg tgttttgtat agaatcatac tgctgctgaa tgaaattgtg
     6301 tatctaaagt cgtaaggcat caggtaggag ctcaaaatta tgaccataat taaatatatt
     6361 tgttgaaggc tccttgggtc cctgactttc tgagagcagg tgttgtggat ggttaaggca
     6421 aaaagtaaaa cttttacaca caggtttctg tcttgtcatt ttgagaatga ctcagtccat
     6481 tctggaccct ttcttactcc attctgtcac tagaaaccat ctcaccccct cacgcctggc
     6541 cacagcaaac agcattctct cttcacttct gaacccagaa tggctacaga aagggttgct
     6601 ggtgcctcat tcaggcggat aggaaggacc acacacagtc aggcctgagc aacaatgtac
     6661 ttctgaagtg tagtaatgaa ctggatgtgc cggggcattg attatatctg cagcttcacc
     6721 ctctgctctt tcttcaaaac acagtctgac tccaagcact tcatacagct tcactgaatg
     6781 ttcctttgaa attaattata aatgactctg aataggcttt gggtagcacc aggcgtcttt
     6841 cagctgacaa tatctagaaa ctcaagttca agaaccaagc cctatatcgt agcttagttt
     6901 tttctactat tagattatag accttcagct acaaaaatta tgtcatttca gaggcttcta
     6961 taatcatagc ttaataatag atataaccca aaaatcatga tgcatgagac tgaattattg
     7021 aagtttaaca tatttctaaa cgatttatgg gcagctcttg atatgaatgt tcctaaacca
     7081 caaccagctg gtttaacata cacataaatt gtgttcatgt gattgaacaa aatttattgt
     7141 taaatgggta agttgacctc agctatggac tcaatttcaa gatcctcgta gtggtgaatg
     7201 aagagcagac actgatatta catacagatt aacaatcttg tcaggctata ctgagatagt
     7261 gtgcaaaatt ccttcctcac acttgatcca aacaattgaa acctgcgtat gactgttctt
     7321 ccatcccttg ttttcttcct tcctttgttc cttctttcct ttaattaaaa aaggtcactg
     7381 atacatttat aacacaggaa gtatcacagc taatcccata ttctaaaata gttttataaa
     7441 gtgattaata tttttagttt gtgtgtatgc atctgtgttt attgaatgta gatgtttcat
     7501 gattgaaaga agtaagtttc tttggaagga cttgggaatg cagagaagta aagtttgtgt
     7561 ctttatttct aatatgttat aaccagatca cggtttcagt taagtacctc attgccatcc
     7621 catccttaag aaatgaccta gttcagtggt tcagctgatc tgcttggaag tatctggctg
     7681 gcttcataag aattccgtag ggagttttag gaatacagaa gactagtctt taccttcaga
     7741 gatttggatt cagtaggtct caggtagggc ctaggaatct gtgtttagaa aatcactcta
     7801 agtaatgcta atatacagcc agacttggga atcattgaaa agtattcaaa taatcccaat
     7861 tactaggaat aaatacaagt tgactgggat agtgatgtta tttcatttgt agttattttg
     7921 aaaagttaaa tactctaggc ctcataccat tatttctttc acaaatttta attttagctt
     7981 tgatttccaa tttgtggtct tctttctgag catgaatctt caaacttaga gacagacgtc
     8041 aacactgata agcatgaggg atgaatattg ctcactatag ggctattagg catactagat
     8101 acagtaccat tttcttagtt actgccagag ctgtgcagta tattacaaca tttgctaatc
     8161 ctcaccaccg aggtaaaaac tatgaccttt gggacacaga agcaaatatc aaagtgacag
     8221 ccttacttct gaatgctttg atttttatgt catgaaaaga catcaactta atacttagtc
     8281 tcactatgta cttttcatat tgaattgtac attatctgaa aactgatttc caagcaatac
     8341 cgtgtaacaa cctttcaagc aataatttaa gccatggtga tgatgccata tgaagcttgc
     8401 acttctttgc atttcataga gcctttgtga aaacagaaac atgttttata caagcaataa
     8461 tatagctggt gtggactttt taaagatact cattattctg tagcaaaatg agcagcaagt
     8521 aatgactcag attaaaaata ttcgggcaaa tgacatgttt aatctatctc agaagttatt
     8581 catttgcaaa catgctgtaa agtgattaat atacatcaga ttatttctgg tgcataattt
     8641 taaacatggc tataaaaata tgatttctat tcctgtcaaa gagatttgtg tattatttat
     8701 aatgttgccc aatttttgga tctttgtagt atataatagt aaggaagaag ttaaggatgg
     8761 ataaatgagt ggaagtagag cataggttgg aagaggtgat tgagaaagta ggctttgcaa
     8821 aaatatagat gggtcttgcg gcagatttat tcaaatgttg gtttgatatg gtcttggaag
     8881 atgaaagagt aaagataaaa gagctcatta ttactgtttt caatgatagc tacacaagtt
     8941 tttggtaagt atgatagtat tttgacttca gaatatgatt gactgaaagt cacctgtagc
     9001 acaataaata aaagtctgaa aatgattatt cagtaatact gaaaaaacac taaagctttc
     9061 agatttcgaa atgaaaaaat catgttttta gtctacttaa aatacaattt taatagaatg
     9121 tggcatagtc aatgataatc agttctttag tagttaaatt tacttcatca tttagaataa
     9181 tgtataagga ttacagctaa aatcaactag taaatttatg tttatactga acagtatcta
     9241 aacatcagat tatgatttag catataagct taggattcat tcctaagcat ctaaataaga
     9301 tgacagctcc tattattgcc ctgcaaggta aacaatcccc taaacatgaa tatgaaccca
     9361 tttgaatggt gggtgtgttc gatattttgt ctttaccttt ctattactca tagcaaaact
     9421 taattttagc attctcctgc ccacagtttg gaaaatttcc ctatcaaaat caaaccggat
     9481 tgttgattta ctgaggcttt gacaattatc ctcttgtata tcactggatc cttaaatcct
     9541 tagtaagaat ggttttgggt taaataaata atggaaaata tttaatttga atctattaaa
     9601 ttacactgaa gttatccttc cagttaattc agctagttgc tgatcagatc attctttata
     9661 ttagacttaa gtattgttgc ttattattta aaagcagaaa agaaaaacca aatggcaggt
     9721 ctgagtaata gttacttttt gattcttttg atacatattt ttaaactgaa gaaatcactt
     9781 tatattcagt cttcttctgt ggactttttg ctatttgcag ataaagaagg aattctactt
     9841 aatatatttt tactgtggtc tattaaatgt cattaaacct atatctaaga ggaaagtaat
     9901 aactgaaata aaatcaggaa tgtgcaaaat tgtttcttca tacctcactt tctgaagaca
     9961 cttctttgca ttctgctgcc tggcatttac acagcagact caggacaaag ggacaaatgg
    10021 agtgagtgag gtgggcagga gctttagaag agatgaagca aagagagtta gttttttatt
    10081 caggaagatg tagagactct taaaggagag gcaatttccg taacaaacac ctctggttta
    10141 atggaagcca catgatttat ttgactactg gtcactcatt tttaaaaaga aaagagaaag
    10201 acaaaaaaag agaaaccctt cacaagccat ttttagtttt cctaggaggt agtgccaacc
    10261 tctcggcagc tgcacatctc agtgaagctg ggatagaagc ttacagagct acaaatagat
    10321 gctttctgaa ggtggcagat agaactagtc ttggttacaa tgattaaggt aaatgaaagt
    10381 gtcagaacat ctgggttgaa ggaacaaaga aggcccaata ccagctctgc catatgccac
    10441 agaaaaccct gctaaaatta gcatagaaaa acagactaac actatgcaag tgatattaaa
    10501 acaaactata ttttttcctt ttataacatg cctggctgag ctagcattca gtgagaagaa
    10561 tatacttttg tcttatgaat catttaaaaa catttgttgt tgttctgaca ttaaataaat
    10621 tacatattcc cttagttgaa aaatttaatc acaaataata ggaaatattt attttgtgtc
    10681 ctgaaatttt taaacttttg gcggctgtac tctacgccat attcttcttc acattcttct
    10741 caaattgggt ttggctgcta gcaaaacaag tgtggaactt tagagcagaa aaatgtccat
    10801 ctgtctctta cagaatgtgc cagagtattt gagctggaga gagaatcacc ccatagcatt
    10861 taatggtatt ctgtgagaca cgaagactct agtcttctaa acttaaaagt ggttaggtag
    10921 taccttaatc atcaaagatt tttcacttac atgtaactgg ttaattcata tagagtttgt
    10981 ttagaacaga aactttaaaa ggagggaaca aacctgagca atttttaatc aaaattatca
    11041 gttagaaaaa gaatttaaga caaagattta ttgtcctgaa agagtcaact cactcatatc
    11101 caaaacttag aatgtcagag acttttgaaa gttttctgaa tgtgtcaact tcttcagcaa
    11161 tgagagattc aattaatgtt gtttctaggt tccttagagt tttatcctct gatgataaca
    11221 cttaattgtg aattacattt aagtgatttt tactttatat tttccatttg ttacagaaaa
    11281 aaagtcataa atatgacaga cttaaaaatt gtggcattga gccagggttt gcaaatttgg
    11341 caatattatt ttacttaaaa tattacactt tttaaaataa cataaacatt aaggataata
    11401 gtcaaataat actgtatttt cagcttgttc acattagaat atagaacaac gcattgtaaa
    11461 cttctcggaa taaaatctaa gtcaaaacca atttatgttc aaatcatttg aacaacaaat
    11521 atgaatattg ttacatcacc tctgactctc tgactagtaa gaaactatgc atttccatac
    11581 ccaatgaaac ctcaattcct acagcaattc tacttttatt gcctcccaag aactatttct
    11641 tcatggtcaa tatcactctt taaaagctag caaaatatga ctttcttctg aggtttgggc
    11701 aatctggatc aatatttatg tatctaacca cttgaaaggc tgtctccttt tttggccttt
    11761 taaattaaaa cttccataaa atgaagcaaa atatgaaaac agctagttga tttatcaata
    11821 atgtgaaacc ctccttctga atttttttgg taaaaattac cacactgaaa ccaaaactta
    11881 accttggctt ggactctcag ataaattgct tttgtattct ttgactacaa agtattctca
    11941 aaaagaagaa attatttctg atggtagatc aaactctagc caagaaggtc tagataaact
    12001 agaggactgt ataattatct aaagtaattc agggggactt ttagaaaaat tctttaattt
    12061 ttgttcctgt gtaaaaatta ttattaatgg atggcagccc aaattactat tcttctttta
    12121 aaatttgttt caagtgtgtc accaggcact cataaaattc atttatttgt tatataactc
    12181 aatgacctga aataataggt gctcattgct ttttcatttg atccttaaaa acaattttat
    12241 tctgtctaaa aaaaattctc ctgctaaaat ccaaatgatt acctggctat aggaggagtc
    12301 ccattttatg aacatgtatg aaatatttag ttggattcag atgaaatgca acttagagaa
    12361 aggaatggat accagtagag aagatggaaa gagacacaaa aatcagactt tgcttacatc
    12421 aaaattaggt ctgttgtcta atcctgttaa cctagaggca agctgaaaat agatggtaag
    12481 gataaataga tttttaaaca cagtatttat atttaacaga atgtcataaa aatgacagaa
    12541 tgccatgaaa ttcagcatgt caaaactttg ataaaacaaa caagaaattt aataaatatg
    12601 ctaattagag tgaatattta aagggacaaa aataacttgt cacatcatag atgtctcccc
    12661 agtgcagaat ttattatctg ctgggaaaat ttggttttat gtcttcaatc aatatttgct
    12721 gcattatcta attaaacatt agtatatttt ttgtgctaat gagaaaccat gcaagagtcc
    12781 aaatatcaag tgtggtgaca gcttgaatca gttgagtttg gtgttcatga ataaaattct
    12841 tgatgaatgt gaaatgaatg gtttatatac ataagtttta tgtgcagtaa attaattagg
    12901 ctttaaaact tgttagcatg cttagctcct cttttttcca ttgaattatc ttcctatctt
    12961 ccttccttcc ttcctttcct tcctttcctt ccctccctct ttgctttatt tctttctagc
    13021 tcttataaga taaatgacaa tagtacttta ctatttgggc tggagcctcc ctttttcact
    13081 ttcacatgat tgaacacttt gtggatttgt gagaaaaggg gaaagaagat gagcacgtag
    13141 atgcagatca gttgaggata gaagtgactg agagaattgg gaccttgact tccagagact
    13201 gtcagtcaag agaaatgtgg gtctataaaa atctatgtgt gttcattttc attgaattta
    13261 aacacttttt caagaagtat cagggtcaat ctgtttggac aatctcctat ttttaccccc
    13321 ttacaaatgt cttaagttag aaaaacctct tattttcaag tagtcatttt tttttatgtg
    13381 agtaccacac tgctaaagac tgaaatagaa aaaaaaaatg ctttttctgt ttgtagggaa
    13441 attgctttct gaaaactact cccaataaga tgtatacatc aaatgcaact tgattttact
    13501 ggctctttat ctagagataa ctgatgcact gtcttctttc ttagataccc ttctgtaatt
    13561 cagcttggca tagtatgatg ttttgagttc ttattgaggg tttggaaaca tttgtcatca
    13621 attttatgtc tactctattg aaaaagcaat atacaatgta ttccatattt cttagctctg
    13681 tgtctttgag gttttaaacc tatattcaga tagctgtttt tagctacaat tttgagtatt
    13741 caccagaatt tacaaattgc cattttatta aatacaaaaa aaaaaaaagt acagcttgga
    13801 tcaagagatc aaacatttac aaatctcaat ccaaagccat tatttttcag ggaaaattaa
    13861 atcatttcta gcagagtatt tgactcctaa gggaaaataa gtcaaatatc ttctttaaga
    13921 gtaagggcat tcttccattg taggtatttc taattgactt tataataatt tgaacaaatc
    13981 ttgaacaaga tttaaagaat ttataatctc ccaaacttaa aattgtattc tttctggttt
    14041 tttattttta tgtattaaga ttatattcaa accacttaat tatcttcatg acataagttt
    14101 gtgttaatag aaaattatac agaaaaaaac atgatttgtt cctctgtgga aagatatgct
    14161 tcaatgagtt aaataccctt gaattttggt tctcatttct tatttggact tttctgttat
    14221 gtacctcagg ggtcttaaat tctaacagat gaagtagtca aaggggtata aaatacttga
    14281 aaaaaattaa aacttcaaaa gggacgaatt tctctgttaa cttttgttta tcctatactg
    14341 acttattttg aaattgtgaa aaaacaggca gctaccatat aaattggtat ctatgaaaat
    14401 actgattact ttcttcctgt gattttcact gttaagaaaa cctttgtctt gaaatgatgg
    14461 acaatcttaa tacttgttca gataaagaaa tgtgactttc aataccttta ccatttgtat
    14521 ttctttaggg aaatatttta ataatgattg taaaaataga agaaatatgg ttgagaaaaa
    14581 tagaagaaat taagatgaaa tttgaacagt tgtaatccac agtcccattt ttccctctat
    14641 tttattgcct tctactaagt tattgaaggc ccagatctgc aaaatttaca agctactaat
    14701 tcattgtctc ctttaagaat ttggagcaaa aattattccg actatgcgga aatgaattca
    14761 catcatcatg gcaggttttg actatgaggg aattcaggtt aggttcaaat agaataatgg
    14821 tccatggaga aaaatacttt ctgatttttc ttcaagttgg atggaaaggt ttttctttat
    14881 ttaaaaaaaa aaaaaaaaag tcctcctgag aattttgcct tcgaaaaaga agttttataa
    14941 cagctgcctg tagctacatt ataacttcgg taattaagtg acaaaactgt ctcattatgc
    15001 ttttaaaata ctgattaagt gcattccaga aataatacat atcattcatt tcaaaatcaa
    15061 cctacctgaa ttctgactct tcaatcatga gtagccattt tacaaaacca aagggtctca
    15121 tttcccaaac agtactttaa atatcatcct aatagtaata tcctaatagt aataatccta
    15181 ttaatactca cttcaatgca taaaataaac agccaatgca cttccatcaa gtctgcaggc
    15241 actatataat aataaatagg tttccaaaat cacttaataa aaacacacag ttataatgtg
    15301 ctcggccttc attagcatcc tctgcatggg gtggttttca tttaacctga attattttaa
    15361 cattttggct aatttgtttg ctgcaatttc atgggtctaa atttttggtt tggtttgcaa
    15421 tgtaaatcat attgtatatc tgctcctttg ttattagatt tactttgtga ataatatctt
    15481 ttaaaaattg agttatcaaa aacttaaatt ataatgtcat caactaatac caggtaaata
    15541 tactaatata tatgtagggc atgattaaat tgcatatatt ttgaaatgtc aaactatttt
    15601 ttgttttttt aaaaaaatga atgcatctct taaacctgta ccattaattt taaaaaagtt
    15661 tcatttggta tttcatatta tattttaaaa tatttttctt tatctagtag tacaggttta
    15721 aagaaacttt ttctgattga aatgataaaa tatgcctcat ttcactagaa ttttaagaat
    15781 aatacatgta aaagtataac tcatataaga ttataaatat ccacaatatc ttctttgatt
    15841 ttcatgtaac accacttttt actcattctt ttatttgtta ttattcgaaa tcattcacaa
    15901 tgtaaatagc atgtttcatg atgtaaaatg tctttgcatt tacagactgc agaacaaggt
    15961 gaatttgtat atgaaaaata agtgatggaa acgtgggatt cttataaaac aaagtggaaa
    16021 tgatagttct gtgttatatt tacataattc attacaaaat tgtctagtta agtcatgcat
    16081 ttatttacct taaaagtgtc caaactgtca cttataaaat atttaaatca ataaataata
    16141 ccaaaaagga
//
